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KR970007350A - 과량의 항원이 없는 단백질의 비탁 및 혼탁 측정법 - Google Patents

과량의 항원이 없는 단백질의 비탁 및 혼탁 측정법 Download PDF

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Publication number
KR970007350A
KR970007350A KR1019960027174A KR19960027174A KR970007350A KR 970007350 A KR970007350 A KR 970007350A KR 1019960027174 A KR1019960027174 A KR 1019960027174A KR 19960027174 A KR19960027174 A KR 19960027174A KR 970007350 A KR970007350 A KR 970007350A
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South Korea
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analyte
reaction
response
sample
sedimentation
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KR1019960027174A
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English (en)
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투엔글러 페터
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부그, 파일
베링베르케 아크티엔게젤샤프트
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Publication of KR970007350A publication Critical patent/KR970007350A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25625—Dilution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
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  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

본 발명은 항체 또는 항체로 피복된 라텍스 물질을 사용하는 항원-매개된 침강 반응후 침강 반응물을 비탁 또는 혼탁 측정에 의해 광학 측정하여 균질 용액으로 단백질을 측정하는 분야에 관한 것이다.

Description

과량의 항원이 없는 단백질의 비탁 및 혼탁 측정법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (15)

  1. a) i) 제1반응에서 샘플의 분획, 바람직하게는 1/5 내지 1/100의 분획을 시약과 항온 처리하여 분석물-의존적 침강 반응을 측정하고, ii) 제2반응에서 나머지 샘플을 단계 i)의 반응 혼합물과 항온 처리하여 분석물-의존적 침강 반응을 측정하는 2단계로 가해지는 샘플을 하나 이상의 분석물-특이적 결합 파트너를 함유하고 입자에 결합될 수 있는 시약과 항온 처리하고, b) 분석물-의존적 침강 반응을 측정하며, c) 측정치를 분석하여 분석물 농도를 측정하는 단계를 포함하여, 분석물-특이적 결합 파트너를 사용하는 분석물-매개된 침강 반응에 의해 생물학적 물질의 샘플중에 존재하는 분석물을 측정하는 면역화학방법.
  2. 제1항에 있어서, 분석물-특이적 결합 파트너가 항체인 방법.
  3. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 혼탁 측정에 의해 수행되는 방법.
  4. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 비탁 측정에 의해 수행되는 방법.
  5. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 역학 측정에 의해 수행되는 방법.
  6. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 고정 시간의 역학 측정에 의해 수행되는 방법.
  7. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 고정치의 측정에 의해 수행되는 방법.
  8. 제1항에 있어서, 분석물-의존적 침강 반응의 측정이 종점 측정에 의해 수행되는 방법.
  9. 제1항에 있어서, 제1반응으로부터 측정된 시그날이 규정된 한계치 이하인 경우, 분석물 농도가 제2반응으로부터 측정된 시그날로부터 계산되는 방법.
  10. 제1항에 있어서, 최초 혼합물에서 제1반응으로부터 측정된 시그날이 규정된 한계치 이상인 경우, 분석물 농도가 샘플 함량이 감소된 새롭게 설정된 분석의 제2반응으로부터 측정된 시그날로부터 계산되는 방법.
  11. 제1항에 있어서, 제1반응으로부터 측정된 시그날이 한계치 이상인 경우, 분석물 농도가 제1반응으로부터 측정된 시그날로부터 계산되는 방법.
  12. 제1항에 있어서, 샘플이 회석되지 않고 사용되는 방법.
  13. 제1항에 있어서, 샘플이 예비회석되는 방법.
  14. 제1항에 있어서, 실측치를 교정 플롯과 비교하여 분석하는 방법.
  15. 제1항에 있어서, 하나는 제1반응 조건하에서 기록되고 다른 하나는 제2반응 조건하에서 기록되는 2개의 교정 플롯을 비교하여 분석하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960027174A 1995-07-05 1996-07-05 과량의 항원이 없는 단백질의 비탁 및 혼탁 측정법 KR970007350A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19524414.1 1995-07-05
DE19524414A DE19524414A1 (de) 1995-07-05 1995-07-05 Antigenüberschußfreie nephelometrische und turbidimetrische Proteinbestimmungen

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KR970007350A true KR970007350A (ko) 1997-02-21

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KR1019960027174A KR970007350A (ko) 1995-07-05 1996-07-05 과량의 항원이 없는 단백질의 비탁 및 혼탁 측정법

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Country Link
US (1) US5789262A (ko)
EP (1) EP0752585B1 (ko)
JP (1) JP3828614B2 (ko)
KR (1) KR970007350A (ko)
AT (1) ATE239229T1 (ko)
AU (1) AU701226B2 (ko)
CA (1) CA2180521C (ko)
DE (2) DE19524414A1 (ko)
ES (1) ES2196104T3 (ko)
MX (1) MX9602623A (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007048536A1 (de) * 2007-10-10 2009-04-16 Zf Friedrichshafen Ag Verfahren zum Betreiben eines Automatgetriebes eines Antriebsstranges
CA2804843C (en) * 2012-02-06 2021-02-02 Ortho-Clinical Diagnostics, Inc. Multiple time windows for extending the range of an assay
EP2790019A1 (de) * 2013-04-10 2014-10-15 Siemens Healthcare Diagnostics Products GmbH High Dose Hook Erkennung

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3861877A (en) * 1974-01-21 1975-01-21 Clinical Technology Inc Optical analysis of fluids
US4157871A (en) * 1976-06-02 1979-06-12 Beckman Instruments, Inc. System for rate immunonephelometric analysis
US4174952A (en) * 1978-01-23 1979-11-20 Massachusetts Institute Of Technology Immunoassay by light scattering intensity anisotropy measurements
FI56905C (fi) * 1978-02-28 1980-04-10 Osmo A Suovaniemi Foerfarande och anordning foer automatisk maetning av agglutinationsprov t ex i spektrofotometer adsoptionsfotometer fluorometer eller nefelometer
US4204837A (en) * 1978-03-14 1980-05-27 Beckman Instruments, Inc. Method of rate immunonephelometric analysis
US4213764A (en) * 1978-08-09 1980-07-22 Akzona Incorporated Method for determining immunochemical substances
JPS5639465A (en) * 1979-09-10 1981-04-15 Olympus Optical Co Ltd Detecting method of immunological agglutination
US4434236A (en) * 1982-10-20 1984-02-28 E. I. Du Pont De Nemours & Co. Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue
US4636479A (en) * 1983-04-20 1987-01-13 Cooper-Lipotech, Inc. Enhanced agglutination method and kit
US4551426A (en) * 1983-10-03 1985-11-05 E. I. Du Pont De Nemours And Company Heterogeneous immunoassay for digoxin using ouabain as a separation means
DE3347162A1 (de) * 1983-12-27 1985-07-04 Behringwerke Ag, 3550 Marburg Photometrisches verfahren zur konzentrationsbestimmung bei reaktionen, die unter bildung oder verbrauch von streuzentren verlaufen
US4847199A (en) * 1987-02-27 1989-07-11 Eastman Kodak Company Agglutination immunoassay and kit for determination of a multivalent immune species using a buffered salt wash solution
US5445936A (en) * 1993-09-15 1995-08-29 Ciba Corning Diagnostics Corp. Method for non-competitive binding assays

Also Published As

Publication number Publication date
EP0752585A3 (de) 1998-07-08
EP0752585B1 (de) 2003-05-02
US5789262A (en) 1998-08-04
JPH0921808A (ja) 1997-01-21
EP0752585A2 (de) 1997-01-08
AU701226B2 (en) 1999-01-21
DE59610394D1 (de) 2003-06-05
ATE239229T1 (de) 2003-05-15
JP3828614B2 (ja) 2006-10-04
AU5832396A (en) 1997-01-16
CA2180521A1 (en) 1997-01-06
DE19524414A1 (de) 1997-01-09
ES2196104T3 (es) 2003-12-16
MX9602623A (es) 1997-06-28
CA2180521C (en) 2009-02-24

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