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KR960041371A - 오르니틴의 제조방법 - Google Patents

오르니틴의 제조방법 Download PDF

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Publication number
KR960041371A
KR960041371A KR1019950011643A KR19950011643A KR960041371A KR 960041371 A KR960041371 A KR 960041371A KR 1019950011643 A KR1019950011643 A KR 1019950011643A KR 19950011643 A KR19950011643 A KR 19950011643A KR 960041371 A KR960041371 A KR 960041371A
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South Korea
Prior art keywords
arginine
production
ornithine
microorganism
medium
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Application number
KR1019950011643A
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English (en)
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KR0161147B1 (ko
Inventor
박영훈
정봉현
최대건
류욱상
장형욱
김남학
Original Assignee
김은영
한국과학기술연구원
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Priority to KR1019950011643A priority Critical patent/KR0161147B1/ko
Publication of KR960041371A publication Critical patent/KR960041371A/ko
Application granted granted Critical
Publication of KR0161147B1 publication Critical patent/KR0161147B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 아르기닌 영양요구성분인 브레비박테리움(Brevibacterium)속의 미생물을 이용한 유가식 발효에 의해 오르니틴을 제조하는 방법에 있어서, 아르기닌을 요구량 이상 첨가한 성장 배지에서 상기 미생물을 배양하여 오르니틴의 생산을 유도한 후, 아르기닌과 인산염 농도를 상기 균주의 아르기닌 영양요구성의 상실을 억제시키는 범위내로 조절되도록 생산용 배지를 공급하여 더욱 배양하는 단계를 포함하는, 생산성이 향상된 오르니틴의 제조 방법에 관한 것으로, 본 발명의 방법에 따르면 65시간의 발효에서 70g/ℓ이상의 오르니틴을 얻을 수 있다.

Description

오르니틴의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (6)

  1. 아르기닌 영양요구성분인 브레비박테리움(Brevibacterium)속의 미생물을 이용한 유가식 발효에 의해 오르니틴을 제조하는 방법에 있어서, 아르기닌을 충분히 첨가한 성장 배지에서 상기 미생물을 배양하여 오르기틴의 생산을 유도한 후, 아르기닌과 인산염 농도를 상기 균주의 아르기닌 영양요구성의 상실을 억제시키는 범위내로 조절되도록 생산용 배지를 공급하여 더욱 배양하는 단계를 포함하는, 생산성이 향상된 오르니틴의 제조 방법.
  2. 제1항에 있어서, 상기 미생물이 브레비박테리움 케토글루타미쿰(Brevibacterium ketoglutamicum) 52(KCTC 0141BP)인 방법.
  3. 제1항에 있어서, 상기 성장 배지에서 상기 미생물을 14 내지 24시간 동안 배양하여 오르니틴의 생산을 유도하는 것을 특징으로 하는 방법.
  4. 제1항에 있어서, 상기 생산용 배지 공급시 배양액중의 아르기닌 농도를 10 내지 40㎎/ℓ로 유지하도록 조절하는 것을 특징으로 하는 방법.
  5. 제1항에 있어서, 상기 생산용 배지 공급시 배양액중의 인산염 농도를 5 내지 60㎎/ℓ로 유지하도록 조절하는 것을 특징으로 하는 방법.
  6. 제1항에 있어서, 상기 생산용 배지중에서의 배양시간이 40 내지 50시간인 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019950011643A 1995-05-12 1995-05-12 오르니틴의 제조방법 KR0161147B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950011643A KR0161147B1 (ko) 1995-05-12 1995-05-12 오르니틴의 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950011643A KR0161147B1 (ko) 1995-05-12 1995-05-12 오르니틴의 제조방법

Publications (2)

Publication Number Publication Date
KR960041371A true KR960041371A (ko) 1996-12-19
KR0161147B1 KR0161147B1 (ko) 1998-11-16

Family

ID=19414267

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950011643A KR0161147B1 (ko) 1995-05-12 1995-05-12 오르니틴의 제조방법

Country Status (1)

Country Link
KR (1) KR0161147B1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100955325B1 (ko) * 2008-01-24 2010-04-29 한국생명공학연구원 신규한 브레비박테리움속 미생물 및 이를 이용한ｌ-오르니친의 제조방법

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100955325B1 (ko) * 2008-01-24 2010-04-29 한국생명공학연구원 신규한 브레비박테리움속 미생물 및 이를 이용한ｌ-오르니친의 제조방법

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Publication number Publication date
KR0161147B1 (ko) 1998-11-16

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