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KR960037838A - Search method for dechlorinated bacteria by plate medium method - Google Patents

Search method for dechlorinated bacteria by plate medium method Download PDF

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Publication number
KR960037838A
KR960037838A KR1019950009507A KR19950009507A KR960037838A KR 960037838 A KR960037838 A KR 960037838A KR 1019950009507 A KR1019950009507 A KR 1019950009507A KR 19950009507 A KR19950009507 A KR 19950009507A KR 960037838 A KR960037838 A KR 960037838A
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South Korea
Prior art keywords
chlorobenzoate
strain
medium
ions
substrate
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KR1019950009507A
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Korean (ko)
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KR0139466B1 (en
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김치경
채종찬
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김치경
하영칠
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Priority to KR1019950009507A priority Critical patent/KR0139466B1/en
Publication of KR960037838A publication Critical patent/KR960037838A/en
Application granted granted Critical
Publication of KR0139466B1 publication Critical patent/KR0139466B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 할로겐하 방향족 화합물을 탈할로겐화하는 기능을 갖는 균주들을 보다 간편하고 효율적으로 검색하기 위하여, 4-클로로벤조에이트를 첨가한 Cl-이온이 배제된 고체 배지에 균주를 접조하고 배양시킨 후, 질산은 용액을 배양 배지위에 분무하여 균 집락 주위에 염화은 침전물이 형성되는 유무를 관찰하는 것으로 행하여지는 방법을 제공한다.In order to more easily and efficiently search for strains having a function of dehalogenating an aromatic compound under halogen, the present invention is prepared by culturing the strain in a solid medium excluding Cl − ions added with 4-chlorobenzoate, A method is provided by spraying a silver nitrate solution on a culture medium to observe the presence of silver chloride precipitates around the colonies.

Description

평판 배지법에 의한 탈염소화 세균의 검색 방법Search method for dechlorinated bacteria by plate medium method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

4-클로로벤조에이트를 참가한 Cl-이온이 배제된 고체 배지에 균주를 접조하고 배양시킨 후, 질산은 용액을 배양 배지위에 분무하여 균 집락 주위에 염화은 침전물이 형성되는 유무를 관찬하는 것으로 행하여지는 할로겐하 방향족 화합물을 탈할로겐화하는 기능을 갖는 균주의 검색 방법.After straining and incubating the strain in a solid medium without Cl − ions participating in 4-chlorobenzoate, the silver nitrate solution was sprayed onto the culture medium to monitor the presence of silver chloride precipitate around the colony. A method for searching for a strain having a function of dehalogenating an aromatic compound. 제1항에 있어서, 기질로 사용되어 지는 4-클로로벤조에이트의 배지중의 함량이 0.5 내지 1.5mM 되도록함을 특징으로 하는 방법.The method according to claim 1, wherein the content of 4-chlorobenzoate to be used as a substrate is 0.5 to 1.5 mM. 제1항에 있어서, 4-클로로벤조에이트를 첨가한 Cl-이온이 배제된 고체 배지에서의 균주의 배양 시간은 2일 이상으로 하는 것을 특징으로 하는 방법.The method of claim 1, wherein the incubation time of the strain in solid medium without Cl − ions to which 4-chlorobenzoate is added is 2 days or more. 제1항에 있어서, 균 집락 부위에 형성되는 염화은 침전물의 직경(㎝)으로 탈할로겐화 활성도를 정의하고, 탈활로겐화 활성도가, 기질의 농도를 0.5 내지 1.5mM으로 하고 배양 시간을 2일로 하였을 때, 1.0 이하를 탈활로겐화 기능이 네카티브(negative)하다고 판단하고, 1.0 이상되는 것만 포지티브(positive)한 것으로 선택하는 것을 특징으로 하는 방법.The dehalogenation activity is defined by the diameter (cm) of silver precipitate formed in the bacterial colonization site, and the dehalogenation activity is defined as the substrate concentration of 0.5 to 1.5 mM and the culture time is 2 days. , 1.0 or less, the dehalogenation function is determined to be negative (negative), and characterized in that it is selected to only positive (1.0) or more. 제1항에 있어서, Cl-이온이 배제된 배지는 K2HOP44g/ℓ, NaH2PO4·2H2O 0.4g/ℓ, (NH4)2SO40.5g/ℓ, MgSO4·7H2O 0.15g/ℓ, CaSO4·2H2O 0.01g/ℓ, 트립톤 0.2g/ℓ, 이스트 익스트랙트 0.1g/ℓ, 아가 1.5％, 로 구성하고 기질로서 4-클로로벤조에이트가 1mM가 첨가되도록 하는 것으로 구성됨을 특징으로 하는 방법.The medium of claim 1, wherein the medium free of Cl − ions is K 2 HOP 4 4 g / l, NaH 2 PO 4 2H 2 O 0.4 g / l, (NH 4 ) 2 SO 4 0.5 g / l, MgSO 4 7H 2 O 0.15g / l, CaSO 4 2H 2 O 0.01g / l, tryptone 0.2g / l, yeast extract 0.1g / l, agar 1.5%, 4-chlorobenzoate as a substrate 1mM Characterized in that it is adapted to be added. ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.※ Note: This is to be disclosed by the original application.
KR1019950009507A 1995-04-22 1995-04-22 Search method for dechlorinated bacteria by plate medium method KR0139466B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950009507A KR0139466B1 (en) 1995-04-22 1995-04-22 Search method for dechlorinated bacteria by plate medium method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950009507A KR0139466B1 (en) 1995-04-22 1995-04-22 Search method for dechlorinated bacteria by plate medium method

Publications (2)

Publication Number Publication Date
KR960037838A true KR960037838A (en) 1996-11-19
KR0139466B1 KR0139466B1 (en) 1998-06-15

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Application Number Title Priority Date Filing Date
KR1019950009507A KR0139466B1 (en) 1995-04-22 1995-04-22 Search method for dechlorinated bacteria by plate medium method

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KR0139466B1 (en) 1998-06-15

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