[go: up one dir, main page]

KR960029343A - 아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법 - Google Patents

아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법 Download PDF

Info

Publication number
KR960029343A
KR960029343A KR1019960002120A KR19960002120A KR960029343A KR 960029343 A KR960029343 A KR 960029343A KR 1019960002120 A KR1019960002120 A KR 1019960002120A KR 19960002120 A KR19960002120 A KR 19960002120A KR 960029343 A KR960029343 A KR 960029343A
Authority
KR
South Korea
Prior art keywords
arginine
protein
interest
extracting
peripheral
Prior art date
Application number
KR1019960002120A
Other languages
English (en)
Other versions
KR100401028B1 (ko
Inventor
르구 리샤르
말도나도 뽈
쌀롬 마르끄
Original Assignee
쥘르 모뒤
사노피
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 쥘르 모뒤, 사노피 filed Critical 쥘르 모뒤
Publication of KR960029343A publication Critical patent/KR960029343A/ko
Application granted granted Critical
Publication of KR100401028B1 publication Critical patent/KR100401028B1/ko
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5437—IL-13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 주변 세포질형 재조합 단백질의 추출제로서 아르기닌을 사용하는 관한 것으로, 특히 관심있는 주변세포질형 단백질의 추출이: (ⅰ) 아르기닌을 포함하는 버퍼 용액 내에서, 원핵 미생물의 주변 세포질에서의 벡터 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양을 통해 생겨나는 세포로부터의 세포데브리 펠럿이나 세포 펠럿을 현탁시키는 단계; 및 (ⅱ) 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어진 주변 세포질형 단백질의 추출방법에 관한 것이다.

Description

아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (7)

  1. 주변세포질형 제조합 단백질의 추출제용으로 사용되는 아르기닌.
  2. 관심있는 주변 세포질형 단백질의 추출방법으로서: -아르기닌을 포함하는 버퍼 용액내에서, 원핵 미생물의 주변세포질에서의 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양물로부터 생겨나는 페포펠럿을 현탁시키는 단계; 및 - 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어지는 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
  3. 관심있는 주변세포질형 단백질의 추출방법으로서: -아르기닌을 포함하는 버퍼 용액내에서, 원핵 미생물의 주변 세포질에서의 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양물로부터 생겨나는 세포로 부터의 세포데브리 펠럿을 현탁시키는 단계; 및 - 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어지는 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
  4. 제2항에 있어서, 아르기닌을 포함하는 버퍼용액이, 0.4M 이상과 동등한 아르기닌 농도를 갖는 알칼리 수용액인 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
  5. 제3항에 있어서, 아르기닌을 포함하는 버퍼용액이, 0.4M 이상과 동등한 아르기닌 농도를 갖는 알칼리 수용액인 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
  6. 관심있는 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양에 있어서, 2g/1 이상과 동등한 농도의 아르기닌 존재하에서 배양이 수행되는 것을 특징으로 하는 원핵 미생물의 배양방법.
  7. 제6항에 있어서, 2g/1와 10g/1 사이 농도의 아르기닌 존재하에서 배양이 수행되는 것을 특징으로 하는 원핵 미생물의 배양방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960002120A 1995-01-31 1996-01-30 아르기닌존재하의원핵미생물로부터주변세포질형단백질의추출방법 Expired - Fee Related KR100401028B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR95-01083 1995-01-31
FR9501083 1995-01-31
FR9501083A FR2729972B1 (fr) 1995-01-31 1995-01-31 Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine

Publications (2)

Publication Number Publication Date
KR960029343A true KR960029343A (ko) 1996-08-17
KR100401028B1 KR100401028B1 (ko) 2004-03-31

Family

ID=9475667

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960002120A Expired - Fee Related KR100401028B1 (ko) 1995-01-31 1996-01-30 아르기닌존재하의원핵미생물로부터주변세포질형단백질의추출방법

Country Status (25)

Country Link
US (2) US5700665A (ko)
EP (1) EP0725140B1 (ko)
JP (1) JP3005464B2 (ko)
KR (1) KR100401028B1 (ko)
CN (1) CN1132842C (ko)
AR (1) AR000831A1 (ko)
AT (1) ATE250132T1 (ko)
AU (1) AU700509B2 (ko)
BR (1) BR9600270A (ko)
CA (1) CA2168382C (ko)
CZ (1) CZ286509B6 (ko)
DE (1) DE69629965D1 (ko)
EA (1) EA000017B1 (ko)
FI (1) FI960427L (ko)
FR (1) FR2729972B1 (ko)
HU (1) HU222598B1 (ko)
IL (1) IL116973A0 (ko)
MX (1) MX9600380A (ko)
NO (1) NO316519B1 (ko)
NZ (1) NZ280919A (ko)
PL (1) PL183598B1 (ko)
SK (1) SK281946B6 (ko)
TW (1) TW403760B (ko)
UA (1) UA27997C2 (ko)
ZA (1) ZA96734B (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1048732A1 (de) 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
KR20010056451A (ko) * 1999-12-15 2001-07-04 윤재승 아르기닌이 강화된 동물세포 배양용 배지 조성물
US20040215139A1 (en) * 2002-12-20 2004-10-28 Todd Cohen Apparatus and method for implanting left ventricular pacing leads within the coronary sinus
CA2633554C (en) 2005-12-22 2012-11-13 Genetech, Inc. Recombinant production of heparin binding proteins
AR062069A1 (es) 2006-07-14 2008-10-15 Genentech Inc Replegado de proteinas recombinantes
GB201107737D0 (en) 2011-05-09 2011-06-22 Univ Birmingham Extraction from cells
WO2022191223A1 (ja) * 2021-03-10 2022-09-15 株式会社カネカ 原核生物細胞から標的タンパク質を抽出する方法
EP4317169A1 (en) 2021-03-22 2024-02-07 Kaneka Corporation Production method for foreign protein using escherichia coli
CN113249391A (zh) * 2021-05-10 2021-08-13 江苏坤力生物制药有限责任公司 一种编码rPD蛋白的核酸、其制备方法和用途

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6244198A (ja) * 1985-08-23 1987-02-26 Toray Ind Inc 有用タンパク質の生産方法
JPS6251983A (ja) * 1985-09-02 1987-03-06 Hagiwara Yoshihide ヒト／ヒト・ハイブリド−マ培養用無血清培地
JPH0672105B2 (ja) * 1985-10-02 1994-09-14 持田製薬株式会社 血栓溶解剤及びその製法
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
JP2769541B2 (ja) * 1987-06-24 1998-06-25 ジェネンテク，インコーポレイテッド 平衡型構成誘導性転写系
FR2636644B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids

Also Published As

Publication number Publication date
ZA96734B (en) 1996-08-16
HU222598B1 (hu) 2003-08-28
AU700509B2 (en) 1999-01-07
US5700665A (en) 1997-12-23
NZ280919A (en) 1997-07-27
DE69629965D1 (de) 2003-10-23
NO960396D0 (no) 1996-01-30
CN1142502A (zh) 1997-02-12
FR2729972B1 (fr) 1997-04-18
SK281946B6 (sk) 2001-09-11
ATE250132T1 (de) 2003-10-15
SK10696A3 (en) 1996-09-04
PL312543A1 (en) 1996-08-05
FI960427A0 (fi) 1996-01-30
EA199600001A3 (ru) 1996-10-01
NO960396L (no) 1996-08-01
KR100401028B1 (ko) 2004-03-31
EP0725140B1 (fr) 2003-09-17
HUP9600209A3 (en) 2000-08-28
HUP9600209A2 (en) 1997-05-28
CZ286509B6 (en) 2000-04-12
JPH08242879A (ja) 1996-09-24
NO316519B1 (no) 2004-02-02
PL183598B1 (pl) 2002-06-28
UA27997C2 (uk) 2000-10-16
EA000017B1 (ru) 1997-12-30
JP3005464B2 (ja) 2000-01-31
TW403760B (en) 2000-09-01
EP0725140A1 (fr) 1996-08-07
HU9600209D0 (en) 1996-03-28
IL116973A0 (en) 1996-05-14
AR000831A1 (es) 1997-08-06
AU4224496A (en) 1996-08-08
EA199600001A2 (ru) 1996-07-01
BR9600270A (pt) 1997-12-23
FR2729972A1 (fr) 1996-08-02
CN1132842C (zh) 2003-12-31
CZ29096A3 (en) 1996-08-14
CA2168382A1 (en) 1996-08-01
MX9600380A (es) 1997-01-31
US5856142A (en) 1999-01-05
FI960427L (fi) 1996-08-01
CA2168382C (en) 2003-09-30

Similar Documents

Publication Publication Date Title
CA2220515C (en) Process for producing erythropoietin containing no animal proteins
KR960029343A (ko) 아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법
NZ330926A (en) Expression augmenting sequence elements (ease) for eukaryotic expression systems
RU94030817A (ru) Днк, белок,вектор, клетка, способ получения продукта
RU94042389A (ru) Промотор, вектор, система экспрессии
KR920701443A (ko) 우레이트 산화효소 활성 단백질, 이 단백질에 대한 재조합 유전자, 발현 벡타, 미생물 및 형질전환세포
ATE356881T1 (de) Expression von alkalischer phosphatase in hefe
KR960010863A (ko) 재조합 머사시딘 및 이의 제조방법
Lee et al. Isolation of a rat mitochondrial release factor. Accommodation of the changed genetic code for termination.
Maia et al. DNA-dependent RNA polymerase from vegetative cells and from spores of Bacillus subtilis: IV. Subunit composition
CA2301044A1 (en) Antimicrobial peptide and dna construct and expression system thereof
DK0953644T3 (da) Systemer til masseproduktion af proteiner eller peptider ved mikroorganismer af slægten Humicola
FR2445338A1 (fr) Preparation de glycoproteine isolee d'enveloppes d'onco-rna virus et vaccins qui la contiennent
DE69504808D1 (de) Corticotropin freisetzende rezeptoren des faktor 2
KR960037830A (ko) 락토-n-바이오시데이즈 유전자
IN187721B (ko)
MX9706290A (es) Secuencias amplificadoras, vectores que portan estas secuencias y sus utilizaciones en composiciones para la expresion de secuencias nucleotidicas en celulas transfectadas, aplicaciones terapeuticas y vacunales.
Yamada et al. Effects of tunicamycin on cell adhesion and biosynthesis of glycoproteins in aggregation-competent cells of Dictyostelium discoideum
US5914390A (en) Methods for increasing yields of recombinant proteins
EP0163573B1 (en) Site-specific proteolysis by renin
KR860002571A (ko) 재조합 폴리펩티드의 분리 방법
FR2634495B1 (fr) Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes
DK0396436T3 (da) Gærstammer til fremstilling af modne heterologe proteiner, især hirudin
Hisano et al. On the self-processing of bacterial alginate lyase
Sledge et al. Costae of Tritrichomonas foetus: purification and chemical composition

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19960130

PG1501 Laying open of application
N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20000811

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20001111

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19960130

Comment text: Patent Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20021127

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20030715

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20030926

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20030926

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
LAPS Lapse due to unpaid annual fee
PC1903 Unpaid annual fee

Termination category: Default of registration fee

Termination date: 20070810