KR960023051A - Method for preparing maltotetraose producing amylase - Google Patents
Method for preparing maltotetraose producing amylase Download PDFInfo
- Publication number
- KR960023051A KR960023051A KR1019940037687A KR19940037687A KR960023051A KR 960023051 A KR960023051 A KR 960023051A KR 1019940037687 A KR1019940037687 A KR 1019940037687A KR 19940037687 A KR19940037687 A KR 19940037687A KR 960023051 A KR960023051 A KR 960023051A
- Authority
- KR
- South Korea
- Prior art keywords
- producing
- starch
- amylase
- maltotetraose
- present
- Prior art date
Links
- 108010065511 Amylases Proteins 0.000 title claims abstract 9
- 102000013142 Amylases Human genes 0.000 title claims abstract 9
- 239000004382 Amylase Substances 0.000 title claims abstract 8
- 235000019418 amylase Nutrition 0.000 title claims abstract 8
- LUEWUZLMQUOBSB-UHFFFAOYSA-N UNPD55895 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(O)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O LUEWUZLMQUOBSB-UHFFFAOYSA-N 0.000 title claims abstract 7
- UYQJCPNSAVWAFU-UHFFFAOYSA-N malto-tetraose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(CO)O1 UYQJCPNSAVWAFU-UHFFFAOYSA-N 0.000 title claims abstract 7
- LUEWUZLMQUOBSB-OUBHKODOSA-N maltotetraose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O[C@@H]3[C@@H](O[C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-OUBHKODOSA-N 0.000 title claims abstract 7
- 229920002472 Starch Polymers 0.000 claims abstract 8
- 235000019698 starch Nutrition 0.000 claims abstract 8
- 239000008107 starch Substances 0.000 claims abstract 8
- 238000004519 manufacturing process Methods 0.000 claims abstract 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract 2
- 229910052799 carbon Inorganic materials 0.000 claims abstract 2
- 239000002361 compost Substances 0.000 claims abstract 2
- 239000002075 main ingredient Substances 0.000 claims abstract 2
- 244000005700 microbiome Species 0.000 claims abstract 2
- FYGDTMLNYKFZSV-DZOUCCHMSA-N alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-D-Glcp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-DZOUCCHMSA-N 0.000 claims 2
- 101710118165 Glucan 1,4-alpha-maltotetraohydrolase Proteins 0.000 claims 1
- 241000187747 Streptomyces Species 0.000 claims 1
- 238000009825 accumulation Methods 0.000 claims 1
- 125000002091 cationic group Chemical group 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 abstract 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 abstract 1
- 229920000856 Amylose Polymers 0.000 abstract 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 abstract 1
- 241000187180 Streptomyces sp. Species 0.000 abstract 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 abstract 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 abstract 1
- 239000001254 oxidized starch Substances 0.000 abstract 1
- 235000013808 oxidized starch Nutrition 0.000 abstract 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/0106—Glucan 1,4-alpha-maltotetraohydrolase (3.2.1.60)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 퇴비로 부터 분리한 말토테트라오즈 생산 아밀라제 효소를 생산하는 새로운 균주인 스트렙토마이세스 속(Streptomyces sp.KSM35)MFCC-10846을 분리하고 전분류를 주원료로 하는 배지에서 호기적으로 배양하여 말토테트라오즈 생산 아밀라제 제조하는 방법에 관한 것으로 특히, 본 발명의 균주는 액화시킨 전분에 일정한 조건에서 작용하여 말토테트라오즈를 주생산물로 생산하며 그외 말토트리오즈 및 말토즈를 생산하며, 탄소원으로 다른 G4-아밀라제 생산 미생물이 용성전분을 주로 이용하는 것에 비해 용성전분 뿐만 아니라 가격이 저렴하고 구입이 용이한 양성전분 및 산화전분을 이용할수 있으며 이들을 주탄소원으로 이용할 때 효소역가가 크게 증가하는 특성이 있어 공업화에 매우 유용한 방법이다.The present invention isolates a novel strain Streptomyces sp.KSM35 MFCC-10846, which produces maltotetraose-producing amylase enzyme isolated from compost, and cultures aerobically in a medium containing starch as a main ingredient. In particular, the present invention relates to a method for producing tetraoz amylase, wherein the strain of the present invention acts on liquefied starch under certain conditions to produce maltotetraose as a main product, and to produce maltotriose and maltose, and other G4 as a carbon source. Compared to amylose producing microorganisms mainly using soluble starch, not only soluble starch, but also cheap starch and easy-to-purchase amphoteric and oxidized starch can be used. This is a very useful way.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019940037687A KR0129471B1 (en) | 1994-12-28 | 1994-12-28 | Method for preparing maltotetraose producing amylase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019940037687A KR0129471B1 (en) | 1994-12-28 | 1994-12-28 | Method for preparing maltotetraose producing amylase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR960023051A true KR960023051A (en) | 1996-07-18 |
| KR0129471B1 KR0129471B1 (en) | 1998-04-04 |
Family
ID=19404110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019940037687A KR0129471B1 (en) | 1994-12-28 | 1994-12-28 | Method for preparing maltotetraose producing amylase |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR0129471B1 (en) |
-
1994
- 1994-12-28 KR KR1019940037687A patent/KR0129471B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR0129471B1 (en) | 1998-04-04 |
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Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 19941228 |
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Patent event code: PA02012R01D Patent event date: 19941228 Comment text: Request for Examination of Application |
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Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 19970930 |
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| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 19971110 Patent event code: PR07011E01D |
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