KR950018483A - 핵산증폭용 프라이머를 이용한 바이러스 진단방법 - Google Patents
핵산증폭용 프라이머를 이용한 바이러스 진단방법 Download PDFInfo
- Publication number
- KR950018483A KR950018483A KR1019930028158A KR930028158A KR950018483A KR 950018483 A KR950018483 A KR 950018483A KR 1019930028158 A KR1019930028158 A KR 1019930028158A KR 930028158 A KR930028158 A KR 930028158A KR 950018483 A KR950018483 A KR 950018483A
- Authority
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- South Korea
- Prior art keywords
- nucleic acid
- acid amplification
- potato
- virus
- crops
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 10
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 8
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 8
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 8
- 230000003321 amplification Effects 0.000 title claims description 6
- 238000003199 nucleic acid amplification method Methods 0.000 title claims description 6
- 238000003745 diagnosis Methods 0.000 title claims description 5
- 238000000034 method Methods 0.000 title claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract 7
- 244000061456 Solanum tuberosum Species 0.000 claims abstract 7
- 206010003694 Atrophy Diseases 0.000 claims abstract 3
- 230000037444 atrophy Effects 0.000 claims abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 2
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 230000009385 viral infection Effects 0.000 claims 4
- 208000036142 Viral infection Diseases 0.000 claims 1
- 230000003612 virological effect Effects 0.000 abstract description 2
- 238000002405 diagnostic procedure Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 특정 DNA서열을 증폭할 수 있는 특성을 이용하여 감자엽권, 감자X, Y 바이러스 및 흑조위축병 바이러스를 특이적으로 검정해낼 수 있는 핵산증식용 프라이며 염기서열 및 이를 이용한 작물의 바이러스 이병 여부의 진단 방법을 제공한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 바이러스 핵산의 분리과정을 도식적으로 나타낸 그림이다.
제2도는 작물에서 추출한 즙액을 이용한 바이러스 정밀 측정농도를 결정하기 위한 전기영등 사진이다.
제3도는 본 발명의 바이러스 진단 방법을 도식적으로 나타낸 그림이다.
제4도는 핵산 대량 증폭용 프라이머를 이용한 바이러스 진단결과를 나타내는 전기영동 사진이다.
Claims (8)
- 하기와 같은 감자엽권바이러스 핵산증폭용 프라이머 염기서열 및 그와 기능적 등가물:
- 하기와 같은 감자 X바이러스 핵산증폭용 프라이머 염기서열 및 그와 기능적 등가물:
- 하기와 같은 감자 Y바이러스 핵산증폭용 프라이머 염기서열 및 그와 기능적 등가물:
- 하기와 같은 흑조위축병 바이러스 핵산증폭용 프라이머 염기서열 및 그와 기능적 등가물:
- 1항의 염기서열을 이용한 작물의 감자엽권과 바이러스감염의 진단방법.
- 2항의 염기서열을 이용한 작물의 감자 X 바이러스감염의 진단방법.
- 3항의 염기서열을 이용한 작물의 감자 Y 바이러스감염의 진단방법.
- 4항의 염기서열을 이용한 작물의 흑조위축병 바이러스감염의 진단방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930028158A KR970011310B1 (ko) | 1993-12-17 | 1993-12-17 | 핵산증폭용 프라이머를 이용한 바이러스 진단방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930028158A KR970011310B1 (ko) | 1993-12-17 | 1993-12-17 | 핵산증폭용 프라이머를 이용한 바이러스 진단방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR950018483A true KR950018483A (ko) | 1995-07-22 |
| KR970011310B1 KR970011310B1 (ko) | 1997-07-09 |
Family
ID=19371380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930028158A Expired - Lifetime KR970011310B1 (ko) | 1993-12-17 | 1993-12-17 | 핵산증폭용 프라이머를 이용한 바이러스 진단방법 |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR970011310B1 (ko) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100846174B1 (ko) * | 2006-12-19 | 2008-07-16 | 대한민국 | 감자에서 발생하는 4종 바이러스의 동시 진단용 프라이머 세트 및 이를 이용한 진단 방법 |
-
1993
- 1993-12-17 KR KR1019930028158A patent/KR970011310B1/ko not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100846174B1 (ko) * | 2006-12-19 | 2008-07-16 | 대한민국 | 감자에서 발생하는 4종 바이러스의 동시 진단용 프라이머 세트 및 이를 이용한 진단 방법 |
Also Published As
| Publication number | Publication date |
|---|---|
| KR970011310B1 (ko) | 1997-07-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 19931217 |
|
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 19931217 Comment text: Request for Examination of Application |
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| PG1501 | Laying open of application | ||
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 19970107 Patent event code: PE09021S01D |
|
| G160 | Decision to publish patent application | ||
| PG1601 | Publication of registration | ||
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 19970922 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 19971208 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 19971208 End annual number: 15 Start annual number: 1 |
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| EXPY | Expiration of term | ||
| PC1801 | Expiration of term |
Termination date: 20140617 Termination category: Expiration of duration |