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KR950018453A - Recombinant Human Growth Hormone with Collagenase Cognitive Site - Google Patents

Recombinant Human Growth Hormone with Collagenase Cognitive Site Download PDF

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Publication number
KR950018453A
KR950018453A KR1019930030318A KR930030318A KR950018453A KR 950018453 A KR950018453 A KR 950018453A KR 1019930030318 A KR1019930030318 A KR 1019930030318A KR 930030318 A KR930030318 A KR 930030318A KR 950018453 A KR950018453 A KR 950018453A
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South Korea
Prior art keywords
growth hormone
human growth
collagenase
recombinant human
expression vector
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KR1019930030318A
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Korean (ko)
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KR100289691B1 (en
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유정권
송영호
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최근선
주식회사 럭키
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Priority to KR1019930030318A priority Critical patent/KR100289691B1/en
Publication of KR950018453A publication Critical patent/KR950018453A/en
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Publication of KR100289691B1 publication Critical patent/KR100289691B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 콜라게네입즈 인지부위를 포함하는 재조합 인간 성장 호르몬 및 이를 코딩하는 유전자를 제공하며, 상기 유전자를 포함하는 발현벡터 및 이에 의해 형질 전환된 미생물을 제공한다. 상기 미생물을 배양하여 콜라게네이즈 인지부위를 포함하는 재조합 인 성장 호르몬을 제조할 수 있으며, 이를 콜라게네이즈로 절단하여 인간 성장 호르몬만을 분리할 수 있다.The present invention provides a recombinant human growth hormone comprising a collagenase recognition site and a gene encoding the same, and provides an expression vector comprising the gene and a microorganism transformed thereby. By culturing the microorganisms can be produced a recombinant phosphorus growth hormone comprising a collagenase recognition site, it can be separated by collagenase can be separated only human growth hormone.

Description

콜라게네이즈 인지부위를 가진 재조합 인간 성장 호르몬Recombinant Human Growth Hormone with Collagenase Cognitive Site

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따른 발현벡터중 콜라게네이즈 인지부위(collagenase recognition site)와 연결된 재조합 인간 성장호르몬의 아미노산 서열 및 이를 코딩하는 재조합 DNA의 염기서열을 나타낸 것이다.Figure 1 shows the amino acid sequence of the recombinant human growth hormone linked to the collagenase recognition site (collagenase recognition site) of the expression vector according to the present invention and the base sequence of the recombinant DNA encoding the same.

제2도는 상기 재조합 DNA의 효모 발현 벡터로의 클로닝 과정을 나타낸 것이다.2 shows the cloning of the recombinant DNA into a yeast expression vector.

제3도는 콜라게네이즈 인지부위를 함유한 재조합 인간 성장 호르몬 발현정도를 15% SDS-PAGE로 나타낸 것이다.3 shows the expression level of recombinant human growth hormone containing collagenase recognition site by 15% SDS-PAGE.

Claims (7)

A-Pro-Gly-Pro-Y-HGH(여기에서 A는 2개 이상의 아미노산 잔기, X및 Y는 각각 1개의 아미노산 잔기, 및 HGH는 인간 성장 호르몬의 아미노산 서열임)로 표시되는, 콜라게네이즈 인지부위를 포함하는 재조합 인간 성장 호르몬.Collagenase, represented by A-Pro-Gly-Pro-Y-HGH, where A is at least two amino acid residues, X and Y are each one amino acid residue, and HGH is the amino acid sequence of human growth hormone Recombinant human growth hormone comprising a cognitive site. 제1항의 재조합 인간 성장 호르몬을 코딩하는 유전자.The gene encoding the recombinant human growth hormone of claim 1. 제2항의 유전자를 포함하는 발현벡터.An expression vector comprising the gene of claim 2. 제3항에 있어서 발현벡터 pYLBC-Alg-UB-HGH.The expression vector pYLBC-Alg-UB-HGH according to claim 3. 제3항의 발현벡터로 형질전환된 미생물.Microorganism transformed with the expression vector of claim 3. 제5항에 있어서, 발현벡터 pYLBC-Alg-UB-HGH로 형질전환된 효모(KCTC 0097BP).The yeast of claim 5 transformed with the expression vector pYLBC-Alg-UB-HGH (KCTC 0097BP). 제5항 또는 제6항 미생물을 배양하여 콜라게네이즈 인지부위를 포함하는 재조합 인간 성장 호르몬을 분리한 후 콜라게네이즈로 절단함을 포함하는, 인간 성장 호르몬의 제조방법.The method for preparing human growth hormone comprising culturing the microorganisms of claim 5 or 6 to separate the recombinant human growth hormone including the collagenase recognition site, and then cutting the collagen into collagenase. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930030318A 1993-12-28 1993-12-28 Recombinant Human Growth Hormone with Collagenase Cognitive Site KR100289691B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930030318A KR100289691B1 (en) 1993-12-28 1993-12-28 Recombinant Human Growth Hormone with Collagenase Cognitive Site

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930030318A KR100289691B1 (en) 1993-12-28 1993-12-28 Recombinant Human Growth Hormone with Collagenase Cognitive Site

Publications (2)

Publication Number Publication Date
KR950018453A true KR950018453A (en) 1995-07-22
KR100289691B1 KR100289691B1 (en) 2001-05-15

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KR1019930030318A KR100289691B1 (en) 1993-12-28 1993-12-28 Recombinant Human Growth Hormone with Collagenase Cognitive Site

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