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KR950003443A - High concentration culture method of photosynthetic bacteria - Google Patents

High concentration culture method of photosynthetic bacteria Download PDF

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Publication number
KR950003443A
KR950003443A KR1019930014242A KR930014242A KR950003443A KR 950003443 A KR950003443 A KR 950003443A KR 1019930014242 A KR1019930014242 A KR 1019930014242A KR 930014242 A KR930014242 A KR 930014242A KR 950003443 A KR950003443 A KR 950003443A
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South Korea
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photosynthetic bacteria
high concentration
culturing
nutrients
basal medium
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KR1019930014242A
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Korean (ko)
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지해성
조정섭
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문승영
두산개발 주식회사
성우경
두산기술원연구조합
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Priority to KR1019930014242A priority Critical patent/KR950003443A/en
Publication of KR950003443A publication Critical patent/KR950003443A/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 호기암의 배양조건에서 고농도 광합성세균을 반연속식으로 배양하는 방법에 관한 것으로, 좀더 구체적으로는 배양초기에는 일정량의 기질을 첨가한 배지를 사용하여 광합성세균을 배양하고, 광합성 세균의 증식에 따라 고농도 기질 및 영양원을 공급하여 적정 pH를 유지하면서 배양을 행함으로써 고농도로 광합성세균의 균체를 증식시킬 수 있는 반연속식 배양방법에 관한 것이다.The present invention relates to a method of culturing semi-continuously high concentration photosynthetic bacteria in culture conditions of aerobic cancer, and more specifically, to culture the photosynthetic bacteria using a medium to which a certain amount of substrate is added in the early stage of culture, The present invention relates to a semi-continuous culture method capable of growing cells of photosynthetic bacteria at high concentration by culturing while maintaining a proper pH by supplying a high concentration substrate and nutrient source according to the growth.

Description

광합성세균의 고농도 배양방법High concentration culture method of photosynthetic bacteria

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 방법에 따라 배양되는 광합성세균의 배양주기를 나타낸 그래프이고, 제2도는 본 발명에 따른 광합성세균의 배양주기와 종래의 광합성세균의 배양주기를 비교도시한 그래프이며, 제3도는 본 발명의 방법과 비교실시예 3의 방법에 따라 배양된 광합성 세균의 균체생산량을 배양시간에 따라 비교도시한 그래프이다.1 is a graph showing the culture cycle of photosynthetic bacteria cultured according to the method of the present invention, Figure 2 is a graph showing the culture cycle of the photosynthetic bacteria according to the present invention and the conventional culture of photosynthetic bacteria, Figure is a graph showing the cell production of the photosynthetic bacteria cultured according to the method of the present invention and Comparative Example 3 according to the culture time.

Claims (6)

광합성세균을 배양하는 방법에 있어서, 휘발성 저급지방산을 배양기의 pH조절기 연등에 의해 기초배지에 반연속적으로 공급하고, 영양소로 질소원, 비타민, 무기물 및 미량원소를 광합성 세균의 건조균체량이 약 2~4g/l 및 15~19g/l일때 공급하면서 배양한 다음, 배양된 광합성세균이 약 40g/l일때 상기 기초배지의 약 2/3를 반연속적으로 회수하고 이와 동시에 별도의 기초배지와 상기 영양소를 공급하여 다시 배양함을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.In the method of culturing photosynthetic bacteria, volatile lower fatty acids are supplied semi-continuously to the basal medium by the pH regulator of the incubator, and nitrogen sources, vitamins, minerals and trace elements as nutrients, and dry cell weight of the photosynthetic bacteria are about 2-4 g. / l and 15-19g / l while culturing while feeding, when the cultured photosynthetic bacteria is about 40g / l to recover about two-thirds of the basal medium semi-continuously and at the same time supply a separate basal medium and the nutrients Method of culturing photosynthetic bacteria at high concentration, characterized in that by culturing again. 제1항에 있어서, 상기 광합성세균을 고농도로 배양후 일부를 회수하고 기질 및 영양소를 첨가한 후, 계속해서 고농도 배양을 반복적으로 행하는 것을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.The method of culturing the photosynthetic bacterium at a high concentration according to claim 1, wherein after the incubation of the photosynthetic bacteria at a high concentration, a part is recovered, the substrate and the nutrients are added, and then the high concentration culture is repeatedly performed. 제1항에 있어서, 상기 질소원이 효모추출물 15~25g 및 트립톤 7~15g을 증류수 75㎖에 용해시킨 용액 및 (NH4)2HPO26.2~11.2g 및 글루탐산소다 28~40g을 증류수 150㎖에 용해시킨 용액으로 구성됨을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.The method according to claim 1, wherein the nitrogen source is a solution in which 15 to 25 g of yeast extract and 7 to 15 g of tryptone are dissolved in 75 ml of distilled water, and 6.2 to 11.2 g of (NH 4 ) 2 HPO 2 and 28 to 40 g of sodium glutamate are 150 ml of distilled water. Method for culturing photosynthetic bacteria at a high concentration, characterized in that consisting of a solution dissolved in. 제1항에 있어서, 상기 비타민이 니코틴산 0.5~2.5g/l, 염산티아민 0.5~2.5g/l 및 비오틴 3~6g/l로 구성됨을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.[Claim 2] The method of claim 1, wherein the vitamin is composed of 0.5-2.5 g / l nicotinic acid, 0.5-2.5 g / l thiamine hydrochloride and 3-6 g / l biotin. 제1항에 있어서, 상기 무기물이 황산마그네슘 1.5~3.6g/l 및 염화칼슘 0.4~0.83g/l로 구성됨을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.The method of claim 1, wherein the inorganic material is cultured at high concentrations photosynthetic bacteria, characterized in that consisting of magnesium sulfate 1.5 ~ 3.6g / l and calcium chloride 0.4 ~ 0.83g / l. 제1항에 있어서, 상기 미량원소가 황산망간 0.8~2.4g/l, 구연산철 2~4g/l 및 염화코발트 0.8~1.8g/l로 구성됨을 특징으로 하는 광합성세균을 고농도로 배양하는 방법.The method of culturing photosynthetic bacteria at high concentration according to claim 1, wherein the trace element is composed of manganese sulfate 0.8-2.4g / l, iron citrate 2-4g / l and cobalt chloride 0.8-1.8g / l. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930014242A 1993-07-26 1993-07-26 High concentration culture method of photosynthetic bacteria KR950003443A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030021577A (en) * 2001-09-06 2003-03-15 이석원 Method patent on photosynthetic bacteria culture

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030021577A (en) * 2001-09-06 2003-03-15 이석원 Method patent on photosynthetic bacteria culture

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