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KR930702680A - Amplified Heterogeneous Chemiluminescent Immunoassay - Google Patents

Amplified Heterogeneous Chemiluminescent Immunoassay

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Publication number
KR930702680A
KR930702680A KR1019930701366A KR930701366A KR930702680A KR 930702680 A KR930702680 A KR 930702680A KR 1019930701366 A KR1019930701366 A KR 1019930701366A KR 930701366 A KR930701366 A KR 930701366A KR 930702680 A KR930702680 A KR 930702680A
Authority
KR
South Korea
Prior art keywords
analyte
specific binding
compound
binding element
mixture
Prior art date
Application number
KR1019930701366A
Other languages
Korean (ko)
Inventor
시.후 로저
에프. 로버트슨 유젠
엠. 몬트고메리 데보라
시. 피터슨 브리안
알리 아크타
피. 마초윅 제임스
엘. 가이딩거 페기
에스. 칼릴 오마
Original Assignee
찰스 엠.브룩
애버트 래보러토리즈
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 찰스 엠.브룩, 애버트 래보러토리즈 filed Critical 찰스 엠.브룩
Publication of KR930702680A publication Critical patent/KR930702680A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

본 발명은 합텐과 같은 증진제 화합물을 포함한 프로브시약을 사용하고 화학발광 시그날 발생화합물을 포함한 결합제를 사용하여 목적하는 반응 시그날을 증폭시키는 화학발광 시그날 증폭방법에 관한 것이다. 또한 이러한 증폭된 화학발광 검정을 수행하기 위한 키트도 제공된다.The present invention relates to a chemiluminescent signal amplification method using a probe reagent containing an enhancer compound such as hapten and amplifying a desired reaction signal using a binder containing a chemiluminescent signal generating compound. Also provided are kits for performing such amplified chemiluminescent assays.

Description

증폭된 불균질 화학발광 면역 검정법Amplified Heterogeneous Chemiluminescent Immunoassay

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (11)

(a)분석물을 포함하는 시험 샘플을 분석물-특이적 결합 쌍요소와 인큐베이팅하여 제1혼합물을 형성시키고, (b)제1혼합물을 분석물/분석물 특이적 결합 요소 쌍컴플렉스를 형성시키기에 충분한 시간 및 조건하에 인큐베이팅 하며, (c)분석물/분석물 특이적 결합 요소 쌍 컴플렉스를 분석물-특이적 결합요소에 결합되는 증진제 화합물을 포함하는 프로브와 접촉시켜 제2혼합물을 형성하고, (d)제2혼합물을 분석물/분석물 특이적 결합요소 쌍/프로브 컴플렉스를 형성시키기에 충분한 시간 및 조건하에 인큐베이팅하며, (e)분석물/분석물 특이적 결합 요소 쌍/프로브 컴플렉서를 증진제-특이적 결합요소에 결합되는 화학발광 시그날 발생화합물을 포함하는 결합체와 접촉시켜 제3혼합물을 형성하고, (f)제3혼합물을 분석물/분석물 특이적 결합 요소 쌍/프로브/결합체 컴플렉서를 형성시키기에 충분한 시간 및 조건하에 인큐베이팅하며, (g)검출 가능한 시그날을 측정하여 시험 샘플 중의 분석물의 존재를 결정함을 특징으로하여, 불균질 면역검정으로부터 발생하는 화학발광 시그날의 특이적 증폭에 의해 시험 샘플중의 분석물의 존재를 결정하는 방법.(a) incubating a test sample comprising the analyte with the analyte-specific binding diurea to form a first mixture, and (b) forming the first mixture with the analyte / analyte specific binding element paircomplex. Incubate under sufficient time and conditions for (c) contacting the analyte / analyte specific binding element pair complex with a probe comprising an enhancer compound bound to the analyte-specific binding element to form a second mixture, (d) incubating the second mixture under time and conditions sufficient to form an analyte / analyte specific binding element pair / probe complex, and (e) analyte / analyte specific binding element pair / probe complexer Contacting a binder comprising a chemiluminescent signal generating compound bound to an enhancer-specific binding element to form a third mixture, and (f) the third mixture is analyte / analyte specific binding element pair / probe / grain Incubating under sufficient time and conditions to form a sieve complex, and (g) measuring the detectable signal to determine the presence of the analyte in the test sample, thereby characterizing the chemiluminescent signal resulting from heterogeneous immunoassay. A method of determining the presence of an analyte in a test sample by enemy amplification. 제1항에 있어서, 분석물이 항체 또는 항원인 방법.The method of claim 1, wherein the analyte is an antibody or antigen. 제1항에 있어서, 증진제 화합물이 합텐, 형광 화합물 및 디-니트로페놀로 이루어진 그룹 중에서 선택되는 방법.The method of claim 1, wherein the enhancer compound is selected from the group consisting of hapten, fluorescent compound, and di-nitrophenol. 제1항에 있어서, 증진제 화합물이 바이오틴인 방법.The method of claim 1, wherein the enhancer compound is biotin. 제1항에 있어서, 화학발광 시그날 발생 화합물이 아크리디늄 에스테르, 아크리디늄 설폰아미드, 1,2-디옥세탄 및 루미놀로 이루어진 그룹중에서 선택되는 방법.The method of claim 1, wherein the chemiluminescent signal generating compound is selected from the group consisting of acridinium esters, acridinium sulfonamides, 1,2-dioxetane and luminol. 제1항에 있어서, 화학발광 시그날 발생 화합물이 아크리디늄 설폰아미드인 방법.The method of claim 1, wherein the chemiluminescent signal generating compound is acridinium sulfonamide. 제1항에 있어서, 분석물-특이적 결합요소를 단계(a)를 수행하기 전에 고상에 결합시키는 방법.The method of claim 1, wherein the analyte-specific binding element is bound to a solid phase prior to performing step (a). 증진제 화합물을 포함한 프로브 시약, 화학발광 시그날 발생 화합물을 포함하는 결합체를 포함하는 결합체를 포함하는 증폭된 화학발광 검정 수행용 키트.A kit for performing an amplified chemiluminescence assay comprising a probe reagent comprising an enhancer compound, a conjugate comprising a conjugate comprising a chemiluminescent signal generating compound. 제8항에 있어서, 증진제 화합물이 합텐, 형광화합물 및 디-니트로페놀로 이루어진 그룹중에서 선택되는 키트.The kit of claim 8 wherein the enhancer compound is selected from the group consisting of hapten, fluorescent compound and di-nitrophenol. 제8항에 있어서, 화학발광 시그날 발생 화합물이 아크리디늄 에스테르, 아크리디늄 설폰아미드, 1,2-디옥세탄 및 루미놀로 이루어진 그룹 중에서 선택되는 키트.The kit of claim 8 wherein the chemiluminescent signal generating compound is selected from the group consisting of acridinium esters, acridinium sulfonamides, 1,2-dioxetane and luminol. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930701366A 1990-11-09 1991-11-08 Amplified Heterogeneous Chemiluminescent Immunoassay KR930702680A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61123590A 1990-11-09 1990-11-09
US07/611,235 1990-11-09
PCT/US1991/008360 WO1992008979A1 (en) 1990-11-09 1991-11-08 Amplified heterogeneous chemiluminescent immunoassay

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KR930702680A true KR930702680A (en) 1993-09-09

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Country Link
EP (1) EP0556331A4 (en)
JP (1) JPH06501559A (en)
KR (1) KR930702680A (en)
AU (1) AU650503B2 (en)
CA (1) CA2095825A1 (en)
TW (1) TW205095B (en)
WO (1) WO1992008979A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0774121A1 (en) * 1994-07-15 1997-05-21 Abbott Laboratories Chemiluminescent signal enhancement
US5705330A (en) * 1995-04-14 1998-01-06 Abbott Laboratories Chemiluminescent immunoassay for antibody detection
US5858665A (en) * 1996-07-25 1999-01-12 Navix, Inc. Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification
ATE417275T1 (en) 1997-09-22 2008-12-15 Novartis Vaccines & Diagnostic BUFFERS FOR STABILIZING HCV ANTIGENS
US6391540B1 (en) 1997-09-22 2002-05-21 Chiron Corporation Method for detecting antibodies in a sample
US6203974B1 (en) 1998-09-03 2001-03-20 Abbott Laboratories Chemiluminescent immunoassay for detection of antibodies to various viruses
EP1412538B1 (en) * 2001-06-26 2013-03-27 Abbott Laboratories Methods for the simultaneous detection of hcv antigens and hcv antibodies
US7101683B2 (en) * 2001-06-26 2006-09-05 Abbott Laboratories Methods for the simultaneous detection of HCV antigens and HCV antibodies
EP2424435B1 (en) 2009-04-30 2021-06-02 Dexcom, Inc. Performance reports associated with continuous sensor data from multiple analysis time periods
WO2012145270A2 (en) 2011-04-20 2012-10-26 Access Medical Systems, Ltd. Luminescent polymer cyclic amplification
CN111781345A (en) * 2020-06-30 2020-10-16 上海透景生命科技股份有限公司 Chemiluminescent marker labeled antigen stabilizer and application thereof

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US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
FR2523311B1 (en) * 1982-03-12 1985-12-27 Pasteur Institut COUPLING PRODUCT BETWEEN AN ALBUMIN AND A SPECIFIC LIGAND, OBTAINING AND APPLICATIONS IN THE BIOLOGICAL FIELD
IL75020A (en) * 1984-05-10 1988-10-31 Abbott Lab Biotin-antibiotin immunoassay for the detection of ligands
US4935339A (en) * 1985-05-07 1990-06-19 Nichols Institute Diagnostics Delayed solid phase immunologic assay
US4927769A (en) * 1987-07-08 1990-05-22 Ciba Corning Diagnostics Corp. Method for enhancement of chemiluminescence

Also Published As

Publication number Publication date
TW205095B (en) 1993-05-01
EP0556331A1 (en) 1993-08-25
JPH06501559A (en) 1994-02-17
CA2095825A1 (en) 1992-05-10
AU9104991A (en) 1992-06-11
AU650503B2 (en) 1994-06-23
EP0556331A4 (en) 1994-10-12
WO1992008979A1 (en) 1992-05-29

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PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19930507

PG1501 Laying open of application
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WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid