KR930021782A - 당근모상근을 이용한 과산화효소의 제조방법 - Google Patents
당근모상근을 이용한 과산화효소의 제조방법 Download PDFInfo
- Publication number
- KR930021782A KR930021782A KR1019920005582A KR920005582A KR930021782A KR 930021782 A KR930021782 A KR 930021782A KR 1019920005582 A KR1019920005582 A KR 1019920005582A KR 920005582 A KR920005582 A KR 920005582A KR 930021782 A KR930021782 A KR 930021782A
- Authority
- KR
- South Korea
- Prior art keywords
- peroxidase
- sucrose
- hairy roots
- medium
- production method
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract 4
- 244000000626 Daucus carota Species 0.000 title claims abstract 3
- 235000002767 Daucus carota Nutrition 0.000 title claims abstract 3
- 102000003992 Peroxidases Human genes 0.000 title claims abstract 3
- 108040007629 peroxidase activity proteins Proteins 0.000 title claims abstract 3
- 229930006000 Sucrose Natural products 0.000 claims abstract 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract 4
- 239000001963 growth medium Substances 0.000 claims abstract 4
- 239000006870 ms-medium Substances 0.000 claims abstract 4
- 239000005720 sucrose Substances 0.000 claims abstract 4
- 150000001413 amino acids Chemical class 0.000 claims abstract 3
- LHGVFZTZFXWLCP-WBJZHHNVSA-N 2-methoxyphenol Chemical class CO[13C]1=[13CH][13CH]=[13CH][13CH]=[13C]1O LHGVFZTZFXWLCP-WBJZHHNVSA-N 0.000 claims abstract 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000004472 Lysine Substances 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims 3
- CDAWCLOXVUBKRW-UHFFFAOYSA-N 2-aminophenol Chemical compound NC1=CC=CC=C1O CDAWCLOXVUBKRW-UHFFFAOYSA-N 0.000 claims 2
- 235000001014 amino acid Nutrition 0.000 claims 2
- 229940024606 amino acid Drugs 0.000 claims 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 claims 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 235000013922 glutamic acid Nutrition 0.000 claims 1
- 239000004220 glutamic acid Substances 0.000 claims 1
- 235000004554 glutamine Nutrition 0.000 claims 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
- 229960001867 guaiacol Drugs 0.000 claims 1
- 235000005772 leucine Nutrition 0.000 claims 1
- 235000018977 lysine Nutrition 0.000 claims 1
- LHGVFZTZFXWLCP-UHFFFAOYSA-N pyrocatechol monomethyl ether Natural products COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 claims 1
- -1 pyrogitol Natural products 0.000 claims 1
- 230000003213 activating effect Effects 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 당근모상근을 이용하여 과산화효소를 생산하는 방법으로, MS배지에 자당 2~5%(W/V)및 리진등의 아미노산 40~70mM을 첨가조성한 1차 성장배양액에서 모상근을 배양한후, 성장생체를 MS 배지에 자당 12~15%(Ｗ/V)및 2- 메톡시페놀등의 유도체 0.2~0.5mM을 첨가조성한 2차 생산배양액에 옮겨 배양 활성화시킴으로서 짧은시간에 대량으로 생산하는 잇점을 제공한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (3)
- 당근모상근을 MS 배지에 자당 2~5%(W/V) 및 아미노산 40~70mM을 첨가하여 조성한 성장 배양액에서 9{12일간 배양한후, 성장 생체를 MS 배지에 자당 12~15%(W/V) 및 유도체 0.2~0.5mM을 첨가하여 조성한 생산 배양액에 옮겨 3~15일간 배양활성화시킴을 특징으로한 과산화효소의 제조방법.
- 제1항에 있어서, 유도체가 2-메톡시페놀, 피로기톨, 카데콜, 2- 아미노페놀 및 2.4-디클로로페놀에서 선택되는 것임을 특징으로한 방법.
- 제1항에 있어서, 아미노산이 리진, 루우신, 글루타민, 글루타민산 및 아스파라진증에서 선택되는 것임을 특징으로한 방법.※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019920005582A KR950012802B1 (ko) | 1992-04-03 | 1992-04-03 | 당근모상근을 이용한 과산화효소의 제조방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019920005582A KR950012802B1 (ko) | 1992-04-03 | 1992-04-03 | 당근모상근을 이용한 과산화효소의 제조방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR930021782A true KR930021782A (ko) | 1993-11-23 |
| KR950012802B1 KR950012802B1 (ko) | 1995-10-21 |
Family
ID=19331343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019920005582A KR950012802B1 (ko) | 1992-04-03 | 1992-04-03 | 당근모상근을 이용한 과산화효소의 제조방법 |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR950012802B1 (ko) |
-
1992
- 1992-04-03 KR KR1019920005582A patent/KR950012802B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR950012802B1 (ko) | 1995-10-21 |
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