KR920002161B1 - Method for preparing dietary fiber derived from diabetes treatment and herbal medicine - Google Patents

Abstract

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Description

Method for preparing dietary fiber derived from diabetes treatment and herbal medicine

FIG. 1 is a change chart of the measured values of blood glucose levels of patients on fasting and 2 hours after eating orally administered dietary fiber (CSP) prepared according to the method of the present invention for 12 weeks.

The present invention relates to an anti-diabetic drug (hypoglycemic agent), and more particularly, to a method for producing dietary fiber derived from 63 herbal drugs and a method for producing an anti-diabetic drug containing the same.

It has been known that aqueous extracts of Anemarrhea asphodeloides Bunge belonging to the family Liliaceae have a hypoglycemic action by oral administration (Japanese clinical trial, vol. 25, p. 2841, 1967 and Japanese Patent Publication No. 1--1). Of course, several synthetic oral hypoglycemic agents and insulin have been used for some time, but these synthetic oral medications have strong side effects such as abnormal hypoglycemia or lactic acidosis. There is a problem in that it is invalid, insulin is best in terms of efficacy, but the administration by injection is inconvenient and accompanied by side effects such as hypoglycemia or allergy, it was preferable to administer under careful management by a doctor.

Even in the situation where there are only drugs with disadvantages and side effects of the administration of such preparations, the number of patients with diabetes increases every year, and the emergence of anti-diabetic medicines with fewer side effects and easier administration methods has been required. In view of the above, the present inventors came to develop a novel antidiabetic medicine of the present invention while studying the development of an excellent antidiabetic medicine.

The present invention is widely used in traditional Chinese medicine, Sosam, Gueun, Tang, etc., widely used for Sogal, Gueun Nutrition, Saengbaekpitang, Mundongumja, Hwanggitang, Hwangyeonjihwangtang, bamboo leaf stone tang, Tanggeoksan, Unlimyunshinhwan, Cheonjiwon, etc. 63 kinds of herbal medicines such as brown root, which is used as a traditional medicine by applying the prescription, such as brown root, hyangbuja, baekbaek-dong, mackmun-dong, astronomical dong, yellow lotus, gardenia, golden, peppermint, bamboo leaf, talc, gypsum, rhubarb, yellow white, halved, white ginseng, licorice , Broiler, Glutinous, Dermis, Jimo, Hansuseok, Sansa, Singok, Jisil, Angelica, Count, Baekryok, Sugiji, Baekchul, Astragalus, Baekbokryeong, Yeonjak, Sanctuary, Breeding, Schisandra chinensis, Forage, Gigolpi, Yeongwa, Hakja, Wooseol, Rosewood incense, Casualties, comparison, Goji, bokbunja, malt, cornus, neck, skin, nutmeg, small white skin, ripe, neck, white porcelain, Seokgok, Cheongung, pedestrians, neck and skin, cheongpi, gilding, royal, earthenware, Seokpo, Yongan Dietary fiber (named CSP) It is to provide a method and an antidiabetic drug containing the same. More specifically, in the present invention, dietary fiber (CSP) is a herbaceous ingredient and composition, which is 0.935-1.265% brown root, 0.935-1.265% hyangbuja, 0.935-1.265% sangbaekpi, 0.935-1.265%, cheonmundong 0.935-1.265 %, Yellow flower 0.935-1.265%, gardenia 0.935-1.265%, golden 0.935-1.265%, mint 0.935-1.265%, bamboo leaf 0.935-1.265%, talc 1.87-2.53%, gypsum 1.87-2.53%, rhubarb 4.25-5.75%, Yellow white 0.935-1.265%, half 0.935-1.265%, white ginseng 0.935-1.265%, licorice 0.935-1.265%, broiler 0.935-1.265%, kalugin 4.25-5.75%, dermis 0.935-1.265%, jimo 0.935-1.265%, Hanseok 0.935-1.265%, Sansa 0.935-1.265%, New Song 0.935-1.265%, Fruits 0.935-1.265%, Angelica 4.25-5.75%, Earl of 4.25-5.75%, Sukji-hwang 1.87-2.53%, Baekchul 0.935-1.265%, Astragalus 0.935- 1.265%, Baekbokryeong 0.935-1.265%, Yeonjak 0.935-1.265%, Poisonous 2.635-3.565%, Breeding 0.935-1.265%, Schisandra chinensis 0.935-1.265%, Forget-me-not 0.935-1.265%, Phalanx 0.935-1.265%, Yeongyo 0.935-1.265% , Thickening 0.935-1.265%, right side 0.935-1.265%, rosewood 2.635-3.565%, Box 0.935-1.265%, compared with 0.935-1.265%, wolfberry 0.935-1.265%, Bokbunja 0.935-1.265%, malt 0.935-1.265%, Cornus 0.935-1.265%, Bark skin 0.935-1.265%, Nutmeg 0.935-1.265%, Sobaekpi 0.935-1.265%, ripe 0.935-1.265%, neck 0.935-1.265%, white porcelain 0.935-1.265%, Seokgok 0.935-1.265%, Cheongung 4.25-5.75%, pedestrian 0.935-1.265%, throat and 0.935-1.265%, cheongju 0.935 -1.265%, gold and silver coins 0.935-1.265%, yellow gold 4.25-5.75%, earthenware 0.935-1.265%, Seokchangpo 0.935-1.265%, dragon meat 0.935-1.265% Extracted with an organic solvent, the residue was centrifuged, dried and pulverized.

In other words, the crude drug was extracted with reflux with ethanol and centrifuged to remove the residue. After adding calcium chloride solution and α-amylase and warming, the pH was adjusted to 3.8-4.8, after which glucoamylase was added and centrifuged. And a method of producing a herbal medicine-derived dietary fiber, characterized in that the residue is centrifuged by drying with purified water and then subjected to purified water, and a method of formulating the dietary fiber so prepared for use as an anti-diabetic drug. do.

The herbal fiber-derived dietary fiber prepared according to the present invention has a hyperglycemic inhibitory effect as shown in FIG.

The dietary fiber proposed in the present invention is composed of polysaccharides, cellulose, hemicellulose and lignin, which are structural polymers claimed by Eastwood and Mitchell. When taken, the volume effect claimed by Jenkis, Shima, etc. gives a feeling of fullness, making it easy to observe meal therapy, and delaying the absorption of nutrients, especially sugars, thus preventing the appearance of hyperglycemia. It is a herbal fiber derived dietary fiber.

Hereinafter, the Example of this invention is described.

Example 1

The herbal medicine roots 11g, Hyangbuja 11g, lettuce white 11g, Mcmundong 11g, astronomical 11g, yellow ginseng 11g, gardenia 11g, golden 11g, mint 11g, bamboo leaf 11g, talc 22g, gypsum 22g, rhubarb 50g, yellow white 11g, half g 11g, white ginseng 11g, Licorice 11g, broiler 11g, glutinous 50g, dermis 11g, gimo 11g, Hansuseok 11g, hawthorn 11g, new song 11g, fruit room 11g, Angelica 50g, Baekjak 50g, Sukjiwang 22g, Baekchul 11g, Astragalus 11g, Baekbokyeong 11g, Yeonjak 11g 31g, 11g for breeding, 11g for Schisandra chinensis, 11g forget-me-not, 11g, Golgolpig 11g, Yeongyo 11g, Thick 11g, Walnut 11g, Rosewood fragrance 31g, Casuall 11g, 11g, Wolfberry 11g, Bokbunja 11g, Malt 11g, Cornus 11g, Bark 11g, Nutmeg 11g, 11g bovine skin, 11g raw, 11g barrel, 11g white porcelain, 11g, Seokgok 11g, Cheongung 50g, almond 11g, neck and 11g, Cheongpi 11g, gold and silver 11g, yellow gold 50g, earthenware 11g, 11g Seokchangpo, 11g longan meat at 55 ℃ After adjusting the water content to about 7% or less, cut it with a control, and weigh 1000g of it, and add 85% of ethanol 6 Add liters, install reflux condenser on the water, extract for 2 hours, centrifuge and wash the residue with purified water three times.

To this, add 3 times of 0.002M calcium chloride (CaCl ₂ ) solution and 20 g of α-amylase and warm it at 90 ° C. for 40 minutes, adjust acetic acid to pH 4.5 and add 40,000 units of glucoamylase to 40 After maintaining at constant temperature for 70 hours at 70 ℃ centrifugation (repeat 3 times or more), and again adjusted to pH 7 with sodium hydroxide (NaOH) and added 3 times the pH 8 phosphate buffer solution to the residue for 6 hours at 35 ℃ The solution is kept at a constant temperature and then centrifuged (repeated two times or more with purified water), and the residue is dried at 85 DEG C (water content of 7% or less) to obtain semal (yield 77%).

Example 2-3

As a result of changing the composition and treatment method for each embodiment was able to prepare a CPS dry powder as follows.

The dietary fiber (CSP) end obtained in accordance with the present invention is administered in a formulation containing at least one pharmaceutically acceptable carrier, the composition of which depends on the solubility, chemical properties, route of administration, dosing schedule, etc. of the compound.

For example, the present CSP is in the form of granules, tablets, capsules, pills, liquids or suspensions containing one or more suitable excipients, stabilizers, sweeteners, pH adjusters, suspending agents, fragrances, such as starch, lactose, and sucrose. Administered orally. The dosage and method of administration of C.S.P is determined by the condition of the patient. The therapeutic amount is usually 50-1500 mg / kg / day by oral administration.

In the acute toxicity experiment, male mice (average body weight 20 g) were orally administered to 7 mice at 5,000 mg / kg in 5% suspension of C.S.P. Thus, it can be seen that the acute toxicity of the product according to the invention is negligible.

Hereinafter, the preparation examples and clinical trial results of the present invention will be described.

[Example 1]

Granules

20 g of methyl cellulose was added to 200 g of the dietary fiber (CSP) powder obtained in Example 1, mixed well, and then compressed and ground in a Laura compactor to form granules. Dosage should be taken after meals 4.6-9.2g three times a day for adults and once a symptom.

[Example 2]

refine

200 g of dietary fiber (CSP) obtained in Example 1 was added 20 g of methyl cellulose and 5 g of magnesium stearate, which were mixed well into a Laura compactor. The granules obtained by compression and crushing were directly compressed to make 760 mg tablets. The dose should be taken 6-12 tablets three times a day for adults and once a symptom.

[Example 3]

Capsule

10 g of calcium carboxymethyl cellulose (calcium CMC) was added to 200 g of the dietary fiber (CSP) powder obtained in Example 1, and the powdery granules, which were compressed and pulverized in a Laura compactor, separated into 20 meshes, were placed into hard capsules. Filling 500mg each to prepare a hard capsule. Dosage should be taken 9-18 capsules 3 times a day for adults and once depending on symptoms.

[Example 4]

Pill

To 200 g of dietary fiber (CSP) horse obtained in Example 1, 5 g of corn starch and 60 ml of water were added and refilled according to a conventional method, followed by drying in a 70 ° C. drying chamber to form pills. The weight of one ring is 90 mg. Dosage should be taken after meals 4.6-9.2g three times a day for adults and once a symptom.

[Example 5]

Liquid

To 200 g of the dietary fiber (CSP) powder obtained in Example 1, 65 g of carboxymethyl cellulose (CMC), sweetener, and fragrance are added in an appropriate amount to make 3,260 ml of water, followed by stirring well in a stirrer to prepare a suspended solution. Dosage should be taken after meals 75ml 3 times a day for adults.

[Example 6]

Suspension

To 200 g of the dietary fiber (CSP) powder obtained in Example 1, 65 g of carboxymethyl cellulose (CMC), sweetener, and fragrance are added in an appropriate amount, and water is added to make 860 ml, followed by stirring in a stirrer to make a suspension. Dosage should be taken 20-40ml three times a day for adults and once a day depending on symptoms.

[Clinical test]

Ten patients with diabetes were administered 4.6 g of CSP granules prepared according to Formulation Example 1 three times a day for 12 weeks, and blood samples were taken on an empty stomach and 2 hours after a meal, and then V-glucose for measuring Glucose (Japan, The results of measuring the amount of glucose in serum before and after administration using the kit were described in Table 1 below.

TABLE 1

Given that the normal blood glucose level is 100 mg / dl or less on an empty stomach and 120 mg / dl or less 2 hours after a meal, the present invention provides blood glucose levels in almost all patients when 8 weeks are administered as shown in Table 1 above. It can be seen that the lowering to the close to normal, and 12 weeks of administration showed that it maintains the normal blood sugar level, the antidiabetic drug of the present invention proved to be a very good drug.

For reference, it is easy to confirm that the average value in the graph shown in Table 1 is shown in FIG. 1 as FIG. 1 and maintained in a very normal blood glucose level after 8 weeks of administration.

Therefore, it can be seen that the present invention is an industrially very useful invention.

Claims (3)

Brown root, hyangbuja, baekbaekpi, makmundong, astronomical dong, yellow lotus, gardenia, golden, peppermint, bamboo leaf, talc, gypsum, rhubarb, yellow baek, half, white ginseng, licorice, broiler, gwalyeoin, dermis, jimo, hansuseok, sansa, new song, jisil , Angelica, Earl of Peony, Sukjihwang, Baekchul, Astragalus, Baekbokryeong, Yeonjuk, Poisonous medicine, Breeding, Schisandra chinensis, Forget-me-not, Peel, Peel, Yeonjak, Habakja, Rosewood incense, Casualties, Compared to wolfberry, Bokbunja, Malt, Cornus, Barley skin, Nutmeg, Sobaek Blood, ripe, neck, white porcelain, Seokgok, Cheongung, passersby, throat, green skin, silver coin, yellow flower, earth and sand, Seokchangpo, longan meat herbal reflux extract and centrifuged the calcium chloride solution and α-amylase Method of producing a herbal medicine-derived dietary fiber, characterized in that after adding and warming to adjust the pH to 3.8-4.8, and then add glucoamylase, centrifugation, drying the residue and washing. According to claim 1, wherein the composition ratio of each herbal medicine is 0.935-1.265%, Hyangbuja 0.935-1.265%, Sangbaekpi 0.935-1.265%, Mcmun-dong 0.935-1.265%, Cheonmun-dong 0.935-1.265%, Hwangnyeon 0.935-1.265%, Gardenia 0.935 -1.265%, gold 0.935-1.265%, mint 0.935-1.265%, bamboo leaf 0.935-1.265%, talc 1.87-2.53%, gypsum 1.87-2.53%, rhubarb 4.25-5.75%, yellow white 0.953-1.265%, halves 0.935-1.265 %, White Ginseng 0.935-1.265%, Licorice 0.935-1.265%, Broiler 0.935-1.265%, Gwalior 4.25-5.75%, Dermis 0.935-1.265%, Jim 0.935-1.265%, Hanseok 0.935-1.265%, Sansa 0.935-1.265% , New Song 0.935-1.265%, New Song 0.935-1.265%, Fruit 0.935-1.265%, Angelica 4.25-5.75%, Baekjak 4.25-5.75%, Sukji Hwang 1.87-2.53%, Baekchul 0.935-1.265%, Astragalus 0.935-1.265%, Baekbokyeong 0.935-1.265%, lotus root 0.935-1.265%, mountain medicinal 2.635-3.565%, breeding 0.935-1.265%, Schisandra chinensis 0.935-1.265%, forget-me-not 0.935-1.265%, phalanges hull 0.935-1.265%, edible 0.935-1.265%, thick pepper 0.935- 1.265%, right side 0.935-1.265%, rosewood 2.635-3.565%, casualties 0.935-1.265%, compared with 0.935-1 .265%, Wolfberry 0.935-1.265%, Bokbunja 0.935-1.265%, Malt 0.935-1.265%, Cornus 0.935-1.265%, Bark skin 0.935-1.265%, Nutmeg 0.935-1.265%, Soybean skin 0.935-1.265%, Ripe 0.935 -1.265%, keg 0.935-1.265%, white porcelain 0.935-1.265%, Seokgok 0.935-1.265%, Cheongung 4.25-5.75%, passercage 0.935-1.265%, neck and 0.935-1.265%, buffy 0.935-1.265%, gold silver 0.935-1.265 %, Hwangjeong 4.25-5.75%, earthenware 0.935-1.265%, Seokchangpo 0.935-1.265%, longan meat 0.935-1.265% extraction method. A therapeutic agent for diabetes, comprising at least one pharmaceutically acceptable carrier such as dietary fiber semals, excipients, stabilizers, sweeteners, pH adjusters, suspending agents, and fragrances obtained from the method of claim 1.

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