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KR920001194A - Enzyme electrode activity maintenance method and apparatus - Google Patents

Enzyme electrode activity maintenance method and apparatus Download PDF

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Publication number
KR920001194A
KR920001194A KR1019910009613A KR910009613A KR920001194A KR 920001194 A KR920001194 A KR 920001194A KR 1019910009613 A KR1019910009613 A KR 1019910009613A KR 910009613 A KR910009613 A KR 910009613A KR 920001194 A KR920001194 A KR 920001194A
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enzyme electrode
regeneration
voltage
regeneration voltage
application
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KR1019910009613A
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Korean (ko)
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다까유끼 이또
히데오 가따야마
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야마다 미노루
다이킨 고오교 가부시끼가이샤
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Publication of KR920001194A publication Critical patent/KR920001194A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

내용 없음No content

Description

효소전극 활성 유지방법 및 그 장치Enzyme electrode activity maintenance method and apparatus

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는, 본 발명의 효소 전극 활성 유지방법의 1실시예를 나타내는 흐름도,1 is a flow chart showing one embodiment of the method for maintaining enzyme electrode activity of the present invention;

제2도는, 효소전극의 활성의 변동을 나타내는 도면,2 is a diagram showing variation in activity of an enzyme electrode;

제3도는, 재생시의 인가전압 파형을 나타내는 도면.3 is a diagram showing an applied voltage waveform at the time of reproduction.

Claims (8)

효소전극(1)을 사용해서 측정 대상 물질의 산화반응 또는 환원 반응을 행하게 하고, 반응의 결과 효소 전극으로부터 출력되는 전기 신호에 기초해서 측정 대상 물질의 농도 측정을 행하는 농도측정 장치에 있어서 소정의 시간마다에 효소전극(1)을 활성화시키기 위한 재생 전압을 인가하고, 더구나 측정 동작을 행하기 전에도 재생 전압을 인가하는 것을 특징으로 하는 효소전극 활성 유지방법.Predetermined time in the concentration measuring apparatus which uses the enzyme electrode 1 to perform the oxidation reaction or the reduction reaction of a substance to be measured, and measures the concentration of the substance to be measured based on the electrical signal output from the enzyme electrode as a result of the reaction. A regeneration voltage for activating the enzyme electrode (1) is applied to each time, and further, a regeneration voltage is applied even before the measurement operation is performed. 제 1항에 있어서, 소정의 시간 마다에 재생 전압 인가가 농도 측정전의 재생 전압 인가를 고려해서 행해지는 것을 특징으로 하는 효소전극 활성 유지방법.The method according to claim 1, wherein the regeneration voltage is applied every predetermined time in consideration of the regeneration voltage applied before the concentration measurement. 제 1항에 있어서, 소정의 시간마다의 재생 전압 인가가 농도 측정전의 재생전압 인가를 고려하는 일이 없이 행해지는 것을 특징으로 하는 효소전극 활성 유지방법.2. The method according to claim 1, wherein the application of the regeneration voltage for each predetermined time is performed without considering the application of the regeneration voltage before the concentration measurement. 제 1항에 있어서, 소정의 시간 마다의 재생 전압 인가를 행한후 효소전극(1)로부터 출력되는 전기 신호와 소정의 역치( 値)와의 대소를 판별해서 효소전극(1)에 이상이 발생하고 있는가 아닌가를 식별하는 것을 특징으로 하는 효소전극 활성 유지방법.2. The abnormality of the enzyme electrode 1 according to claim 1, wherein the magnitude of the electrical signal output from the enzyme electrode 1 and the predetermined threshold value is determined after application of the regenerative voltage at predetermined time intervals. Enzyme electrode activity maintenance method, characterized in that identifying whether or not. 효소전극(1)을 사용해서 측정 대상 물질의 산화반응 또는 환원 반응을 행하게 하고, 반응의 결과 효소 전극(1)로부터 출력되는 전기 신호에 기초해서 측정 대상 물질의 농도 측정을 행하는 농도 측정 장치에 있어서 효소전극(1)을 활성화 시키기 위한 재생전압을 인가하는 재생전원(2)와 소정의 시간마다에 재생전압을 효소전극(1)에 인가하도록 재생전원(2)와 효소전극(1)을 접속하는 제1의 접속수단과 측정 동작을 행하기 전에 재생 전압을 효소전극(1)에 인가하도록 재생전원(2)와 효소전극(1)을 접속하는 제2의 접속 수단을 포함하는 것을 특징으로 하는 효소전극 활성 유지장치.In the concentration measuring apparatus which performs an oxidation reaction or a reduction reaction of a measurement target substance using the enzyme electrode 1, and measures the density | concentration of a measurement target substance based on the electrical signal output from the enzyme electrode 1 as a result of reaction. A regeneration power supply 2 for applying a regeneration voltage for activating the enzyme electrode 1, and a regeneration power supply 2 for connecting the regeneration power supply 2 and the enzyme electrode 1 to apply the regeneration voltage to the enzyme electrode 1 every predetermined time. And a second connecting means for connecting the regeneration power supply 2 and the enzyme electrode 1 to apply a regeneration voltage to the enzyme electrode 1 before performing the measurement operation with the first connecting means. Electrode active retainer. 제 5항에 있어서, 제 1의 접속 수단이 제 1의 접속 수단에 의한 재생전압의 효소 전극(1)에의 인가 또는 제 2의 접속 수단에 의한 재생전압의 효소전극(1)에의 인가를 기준으로 해서 소정의 시간 경과후에 재생 전압을 인가하는 것을 특징으로 하는 효소전극 활성 유지장치.6. The method according to claim 5, wherein the first connection means is based on the application of the regeneration voltage to the enzyme electrode 1 by the first connection means or the application of the regeneration voltage to the enzyme electrode 1 by the second connection means. And a regeneration voltage is applied after a predetermined time has elapsed. 제 5항에 있어서, 제 1의 접속 수단이 제 1의 접속 수단에 의한 재생 전압의 효소전극(1)에의 인가만을 기준으로 해서 소정의 시간 경과후에 재생 전압을 인가하는 것을 특징으로 하는 효소 전극 활성 유지장치.6. The enzyme electrode activity according to claim 5, wherein the first connection means applies the regeneration voltage after a predetermined time period based only on the application of the regeneration voltage to the enzyme electrode 1 by the first connection means. Retaining device. 제 5항에 있어서, 제 1의 접속 수단에 의해 효소전극(1)에의 재생 전압 인가를 행한것에 응답하여 효소전극(1)로부터 출력되는 전기 신호와 소정의 역치와의 대소를 판별해서 효소전극(1)에 이상이 발생했는가 아닌가를 식별하는 효소전극 상태 식별 수단을 다시 또 포함하고 있는 것을 특징으로 하는 효소 전극 활성 유지장치.The method according to claim 5, wherein the magnitude of the electrical signal output from the enzyme electrode 1 and a predetermined threshold value is determined in response to the application of the regenerative voltage to the enzyme electrode 1 by the first connecting means. An enzyme electrode activity maintaining apparatus, further comprising an enzyme electrode state identification means for identifying whether an abnormality has occurred in 1). ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910009613A 1990-06-12 1991-06-12 Enzyme electrode activity maintenance method and apparatus KR920001194A (en)

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JP90-154527 1990-06-12
JP15452790 1990-06-12

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EP0461601A3 (en) 1993-05-12
DE69115360T2 (en) 1996-06-20

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