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KR900004934A - Method for preparing improved heat resistant alpha-amylase - Google Patents

Method for preparing improved heat resistant alpha-amylase Download PDF

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Publication number
KR900004934A
KR900004934A KR1019880011914A KR880011914A KR900004934A KR 900004934 A KR900004934 A KR 900004934A KR 1019880011914 A KR1019880011914 A KR 1019880011914A KR 880011914 A KR880011914 A KR 880011914A KR 900004934 A KR900004934 A KR 900004934A
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KR
South Korea
Prior art keywords
amylase
heat
resistant alpha
producing
resistant
Prior art date
Application number
KR1019880011914A
Other languages
Korean (ko)
Inventor
박관화
김인철
장소영
차재호
노현모
고영환
Original Assignee
고재군
서울대학교 농과대학 농업개발연구소
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 고재군, 서울대학교 농과대학 농업개발연구소 filed Critical 고재군
Priority to KR1019880011914A priority Critical patent/KR900004934A/en
Publication of KR900004934A publication Critical patent/KR900004934A/en

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Abstract

내용 없음No content

Description

개량된 내열성 라파-아밀라아제의 제조방법Method for preparing improved heat resistant rapa-amylase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

내열성 아밀라아제를 생산하는 대장균을 배양하고 배양물로 부터 내열성 알파 아미라아제를 채취하여 내열성 아밀라아제를 제조하는 방법에 있어서, "바실러스 리케니포미스 ATCC 27811"라는 균주로부터 내열성 알파-아밀라아제를 암호하는 유전자를 대장균 및 고초균등에 형질 전환시키는 것을 특징으로 하는 내열성 알파 아밀라아제의 제조방법.In a method for producing heat-resistant amylase by culturing E. coli producing heat-resistant amylase and extracting heat-resistant alpha amylase from the culture, a gene encoding heat-resistant alpha-amylase from a strain called "Bacillus rickenomyces ATCC 27811" A method for producing a heat resistant alpha amylase, characterized by transforming E. coli and Bacillus subtilis. 제1항에 있어서, 대장균을 이용하여 배양기간 약 1일, 및 배양돈도 30-35℃의 조건하에 제조함을 특징으로 하는 내열성 알파 아밀라아제의 제조방법.[Claim 2] The method for preparing heat-resistant alpha amylase according to claim 1, wherein the Escherichia coli is cultured for about 1 day, and the cultured pig is also prepared under the conditions of 30-35 ° C. 제1항에 있어서, pH 7.5 70℃에서 최적 활성을 보이는 성질을 나타내는 것임을 특징으로 하는 내열성 알파-아밀라아제의 제조방법.According to claim 1, pH 7.5 is a method for producing a heat-resistant alpha-amylase, characterized in that exhibiting properties exhibiting optimum activity at 70 ℃. 제1항에 있어서, 25mM Ca++이온 존재하에서 최적 활성온도가 90℃가 되도록 하는 조건하에서 행함을 특징으로 하는 내열성 알파-아밀라아제의 제조방법.The method for producing a heat resistant alpha-amylase according to claim 1, which is carried out under conditions such that the optimum activity temperature is 90 ° C in the presence of 25mM Ca ++ ions. 제1항에 있어서, pH 6에서 pH 10까지의 사이에서는 안정성의 변화가 없는 조건하에서 행함을 특징으로 하는 내열성 알파-아밀라아제의 제조방법.The method for producing a heat resistant alpha-amylase according to claim 1, which is carried out under a condition in which there is no change in stability between pH 6 and pH 10. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880011914A 1988-09-15 1988-09-15 Method for preparing improved heat resistant alpha-amylase KR900004934A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019880011914A KR900004934A (en) 1988-09-15 1988-09-15 Method for preparing improved heat resistant alpha-amylase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019880011914A KR900004934A (en) 1988-09-15 1988-09-15 Method for preparing improved heat resistant alpha-amylase

Publications (1)

Publication Number Publication Date
KR900004934A true KR900004934A (en) 1990-04-13

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KR1019880011914A KR900004934A (en) 1988-09-15 1988-09-15 Method for preparing improved heat resistant alpha-amylase

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100373172B1 (en) * 1996-10-16 2003-08-19 주식회사 코오롱 Glass fiber reinforced polyamide resin composition

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100373172B1 (en) * 1996-10-16 2003-08-19 주식회사 코오롱 Glass fiber reinforced polyamide resin composition

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Legal Events

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PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19880915

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
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