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KR890701632A - 사람 인터루킨-4에 대한 모노클로날 항체 및 이를 생성하는 하이브리도마 - Google Patents

사람 인터루킨-4에 대한 모노클로날 항체 및 이를 생성하는 하이브리도마

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KR890701632A
KR890701632A KR1019890701139A KR890701139A KR890701632A KR 890701632 A KR890701632 A KR 890701632A KR 1019890701139 A KR1019890701139 A KR 1019890701139A KR 890701139 A KR890701139 A KR 890701139A KR 890701632 A KR890701632 A KR 890701632A
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antibody
human interleukin
monoclonal antibody
specific
hybridoma
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KR930008112B1 (ko
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에스.애브램스 죤
크르티엥 이자벨르
디.리 프랭크
케이.피어스 마이클
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스테이너 브이.칸스태드
쉐링 바이오텍 코포레이션
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Abstract

내용 없음

Description

사람 인터루킨-4에 대한 모노클로날 항체 및 이를 생성하는 하이브리도마
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 클리코실화되지 않은 사람 IL-4를 세균숙주내에서 발현시키기에 적합한 발현벡터를 도식적으로 표시한 것이다. 제2도는 사람 IL-4의 최종 정제단계시 215nm에서의 흡과양상을 나타낸 것이다. 제3도(a 및 b)는 다우디 세포에 결합하는 125I-CHO HuIL-4의 중화정도를 나타낸 것이다.

Claims (26)

  1. 사람 인터루킨-4에 특이적인 모노클로날 항체.
  2. 제1항에 있어서, 비글리코실화된 사람 인터루킨-4에 특이적인 모노클로날 항체.
  3. 제1항에 있어서, 글리코실화된 사람 인터루킨-4에 특이적인 모노클로날 항체.
  4. 제3항에 있어서, 하이브리도마 IC1. 11B4. 6에 의해 생성된 모노클로날 항체.
  5. 제1항에 있어서, 사람 인터루킨-4의 생물학적 활성을 차단할 수 있는 모노클로날 항체.
  6. 제5항에 있어서, 하이브리도마 MP4. 25D2. 11에 의해 생성된 모노클로날 항체.
  7. 사람 인터루킨-4에 특이적인 모노클로날 항체를 분비할 수 있는 하이브리도마.
  8. 제7항에 있어서, 비글리코실화된 사람 인터루킨-4에 특이적인 모노클로날 항체를 분비할 수 있는 하이브리도마.
  9. 제7항에 있어서, 글리코실화된 사람 인터루킨-4에 특이적인 모노클로날 항체를 분비할 수 있는 하이브리도마.
  10. 제9항에 있어서, IC1. 11B4. 6 또는 MP4. 25D2. 11로 명명된 하이브리도마.
  11. 사람 인터루킨-4에 특이적인 모노클로날 항체의 경쇄 변화영역(light chain variable region)을 함유하는 폴리펩타이드.
  12. 제11항에 있어서, 모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성되는 폴리펩타이드.
  13. 사람 인터루킨-4에 특이적인 모노클로날 항체의 중쇄 변화영역(heavy chain variable region)을 함유하는 폴리펩타이드.
  14. 제13항에 있어서, 모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성되는 폴리펩타이드.
  15. 사람 인터루킨-4에 특이적인 모노클로날 항체로부터 유도된 중쇄 변화영역 및 경쇄 사람 인터루킨-4에 특이적인 조성물.
  16. 제15항에 있어서, 모노클로날 항체가 하이브리도마 IC. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성되는 폴리펩타이드.
  17. 사람 인터루킨-4에 대한 제1항원 결정요소(determinant)에 특이적인 제1모노클로날 항체를 제공하고, 사람 인터루킨-4에 특이적인 폴리클로날 항체 조성물 및 사람 인터루킨-4에 대한 제2항원 결정요소(이것은 제1항원 결정요소와는 다르다)에 특이적인 제2모노클로날 항체로 이루어진 그룹에서 선택되는 제2항체를 제공한 후, 제1모노클로날 항체 또는 제2항체와 기능적으로 결합하여 제1모노클로날 항체 또는 제2항체 각각의 양에 대해 일정한 강도의 시그날을 생성할 수 있는 시그날-생성 물질을 제공하고, 제1모노클로날 항체 또는 제2항체를 지지물질에 결합시켜 항체-지지체 연결체를 형성토록 한 후, 시료를 항체-지지체 연결체와 접촉시켜 시료중의 사람 인터루킨-4가 항체-지지체 연결체와 결합하도록 하고, 항체-지지체 연결체가 제2항체를 함유하는 경우, 시그날 생성물질과 기능적으로 결합된 제1모노클로날 항체를, 항체-지지체 연결체와 결합된 사람 인터루킨-4와 접촉시키거나, 항체-지지체 연결체가 제1모노클로날 항체를 함유하는 경우, 시그날 생성물질이 기능적으로 결합된 제2항체를, 항체-지지체 연결체와 결합된 사람 인터루킨-4와 접촉시키고, 시그날 생성물질에 의해 생선된 시그날을 측정하는 단계로 이루어짐을 특징으로 하는, 사람 인터루킨-4를 함유하는 것으로 예측되는 시료중의 사람 인터루킨-4의 존재 판정방법.
  18. 제17항에 있어서, 항체-지지체 연결체가 제1모노클로날 항체를 함유하고, 제2항체가 사람 인터루킨-4에 특이적인 폴리클로날 항체 조성물인 방법.
  19. 제18항에 있어서, 제1모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성되는 모노클로날 항체인 방법.
  20. 제17항에 있어서, 항체-지지체 연결체가 제2항체를 함유하고, 제2항체가 사람 인터루킨-4에 특이적인 폴리클로날 항체 조성물인 방법.
  21. 제20항에 있어서, 제1모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 하이브리도마 MP4. 25D2. 11에 의해 생성되는 모노클로날 항체인 방법.
  22. 사람 인터루킨-4에 대한 제1항원 결정요소에 특이적인 제1모노클로날 항체; 사람 인터루킨-4에 대해 특이적인 폴리클로날 항체 조성물 및 사람 인터루킨-4에 대한 제2항원 결정요소(이것은 제1항원 결정 요소와는 다르다)에 특이적인 제2모노클로날 항체로 이루어진 그룹에서 선택되는 제2항체; 지지체 물질; 및 시그날 생성물질로 이루어짐을 특징으로 하는, 사람 인터루킨-4를 함유하는 것으로 예측되는 시료중의 사람 인터루킨-4의 존재 판정용 키트.
  23. 제22항에 있어서, 제1모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성돈 모노클로날 항체이고, 제2항체가 사람 인터루킨 4에 특이적인 폴리크로날 항체 조성물인 키트.
  24. 제23항에 있어서, 시그날 생성물질이 제1모노클로날 항체와 기능적으로 결합되 효소를 함유하며, 이 효소가 vdhr시다제, 베타-갈락토시다제, 및 알카리성 포스파타제로 구성된 그룹에서 선택되는 효소인 키트.
  25. 사람 인터루킨-4에 특이적인 모노클로날 항체를 함유하는 면역흡착 컬럼에 통과시킨, 사람 인터루킨-4 함유시료로부터 사람 인터루킨-4를 추출하기 위한 면역 정제방법.
  26. 제25항에 있어서, 모노클로날 항체가 하이브리도마 IC1. 11B4. 6 또는 MP4. 25D2. 11에 의해 생성되는 면역 정제방법.
    ※ 참고방법: 최초출원 내용에 의하여 공개하는 것임.
KR1019890701139A 1987-10-26 1988-10-21 사람 인터루킨-4에 대한 모노클로날 항체 및 이를 생성하는 하이브리도마 KR930008112B1 (ko)

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Application Number Priority Date Filing Date Title
US07/113,623 US5041381A (en) 1986-07-03 1987-10-26 Monoclonal antibodies against human interleukin-4 and hybridomas producing the same
US?113,623? 1987-10-26
US113623 1987-10-26
PCT/US1988/003631 WO1989003846A2 (en) 1987-10-26 1988-10-21 Monoclonal antibodies against human interleukin-4 and hybridomas producing the same

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KR890701632A true KR890701632A (ko) 1989-12-21
KR930008112B1 KR930008112B1 (ko) 1993-08-26

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CN (2) CN1032816A (ko)
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Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5700915A (en) * 1985-11-19 1997-12-23 Schering Corporations Human interleukin immunopurification processes
WO1989006975A1 (en) * 1988-02-02 1989-08-10 Schering Biotech Corporation Method of reducing immunoglobulin e responses
CA2071872A1 (en) * 1989-12-15 1991-06-16 Abla A. Creasey Cytokine antibody for the treatment of sepsis
GB8928501D0 (en) * 1989-12-18 1990-02-21 Unilever Plc Reagents
HU215245B (hu) * 1989-12-20 1998-11-30 Schering Corp. Eljárás a humán interleukin-4 (IL-4) polipeptidek, ezekkel termelt antitestek és az antitesteket tartalmazó gyógyszerkészítmények előállítására
US5132109A (en) * 1990-10-05 1992-07-21 Ludwig Institute For Cancer Research Method for inhibiting production of ige and method for enhancing production of igg using interleukin 9 and inhibitors thereof
DE4137333A1 (de) * 1991-11-13 1993-05-19 W Prof Dr Sebald Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung
CA2130436C (en) * 1992-02-19 2001-04-10 John S. Abrams Cloning and expression of humanized monoclonal antibodies against human interleukin-4
US5714146A (en) * 1992-08-26 1998-02-03 Board Of Regents Of The University Of Washington IL-4 bone therapy
US5928904A (en) * 1993-09-07 1999-07-27 Smithkline Beecham Corporation DNA encoding recombinant IL4 antibodies useful in treatment of IL4 mediated disorders
CN1473854A (zh) * 1993-09-07 2004-02-11 ʷ��˿�������ȳ�ķ���޹�˾ 用于治疗il4介导疾病的重组il4抗体
US20020193575A1 (en) * 1993-09-07 2002-12-19 Smithkline Beecham P.L.C. Recombinant IL4 antibodies useful in treatment of IL4 mediated disorders
US5914110A (en) * 1993-09-07 1999-06-22 Smithkline Beecham Corporation Recombinant IL4 antibodies useful in treatment of IL4 mediated disorders
US5959085A (en) * 1993-11-23 1999-09-28 Schering Corporation Human monoclonal antibodies against human cytokines and methods of making and using such antibodies
EP0659766A1 (en) * 1993-11-23 1995-06-28 Schering-Plough Human monoclonal antibodies against human cytokines and methods of making and using such antibodies
WO1995021942A1 (en) * 1994-02-15 1995-08-17 T Cell Diagnostics, Inc. Immunoassay kit comprising universal reagents
US5597710A (en) * 1994-03-10 1997-01-28 Schering Corporation Humanized monoclonal antibodies against human interleukin-4
US5705154A (en) * 1995-03-08 1998-01-06 Schering Corporation Humanized monoclonal antibodies against human interleukin-4
AUPN481295A0 (en) * 1995-08-16 1995-09-07 Medvet Science Pty. Ltd. Agonists of haemopoietic growth factors
US20020090682A1 (en) * 1995-10-23 2002-07-11 Tracy Willson Novel haemopoietin receptor and genetic sequences encoding same
DK2990420T3 (en) 2000-05-26 2017-04-03 Immunex Corp USE OF INTERLEUKIN-4 RECEPTOR ANTIBODIES AND COMPOSITIONS THEREOF
GB0210535D0 (en) * 2002-05-08 2002-06-19 Novartis Ag Organic compounds
JP2004045384A (ja) * 2002-05-22 2004-02-12 Matsushita Electric Ind Co Ltd 免疫学的測定方法、免疫学的測定装置、生体成分測定用トイレ、抗アルブミンモノクローナル抗体、それを産生する細胞株、およびアルブミン検出キット
US20070104710A1 (en) * 2002-06-28 2007-05-10 Domants Limited Ligand that has binding specificity for IL-4 and/or IL-13
US20110223168A1 (en) * 2002-12-27 2011-09-15 Greg Winter Ligand that has binding specificity for il-4 and/or il-13
PE20060560A1 (es) * 2004-08-03 2006-06-27 Novartis Ag Anticuerpos de interleucina-4 humana
US7608693B2 (en) * 2006-10-02 2009-10-27 Regeneron Pharmaceuticals, Inc. High affinity human antibodies to human IL-4 receptor
GB0807214D0 (en) * 2008-04-21 2008-05-28 Belfast Health And Social Care Biomarkers
GB201210571D0 (en) * 2012-06-14 2012-08-01 Belfast Health And Social Care Trust Predictive biomarker
GB201210587D0 (en) * 2012-06-14 2012-08-01 Belfast Health And Social Care Trust Predictive biomarker
CN103421747B (zh) * 2013-09-09 2015-01-07 扬州大学 分泌牛il-4单克隆抗体的杂交瘤细胞株、其单克隆抗体及其应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4255329A (en) 1973-10-02 1981-03-10 Syva Company Double receptor fluorescent immunoassay
US4342832A (en) 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4431739A (en) * 1979-11-05 1984-02-14 Genentech, Inc. Transformant bacterial culture capable of expressing heterologous protein
US4423147A (en) 1980-04-11 1983-12-27 Secher David S Monoclonal antibody to interferon-α
ATE5541T1 (de) 1980-06-20 1983-12-15 Battelle Memorial Institute Verfahren zur delignifizierung von holz und anderen lignocellulosematerialien.
US4486530A (en) 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4355023A (en) 1980-09-30 1982-10-19 The Massachusetts General Hospital Antibody fragment compositions and process
US4599308A (en) 1981-10-06 1986-07-08 Hamer Dean H Protein from SV40 recombinants
US4470925A (en) 1982-05-05 1984-09-11 E. I. Du Pont De Nemours And Company Immunoglobulin half-molecules and process for producing hybrid antibodies
US4474493A (en) 1982-09-09 1984-10-02 Modular Systems, Inc. Dowel fastener and joints including same
EP0189405B1 (en) 1983-09-15 1989-02-22 University Of Bath Disinfection of contact lenses
US4731237A (en) 1983-11-07 1988-03-15 The Wistar Institute Immune response to virus induced by anti-idiotype antibodies
EP0190205B1 (en) 1984-07-24 1992-10-21 Coselco Mimotopes Pty. Ltd. Method for determining mimotopes
US4562003A (en) 1984-10-26 1985-12-31 California Biotechnology, Inc. Monoclonal antibodies against alveolar surfactant protein
EP0190711B1 (en) 1985-02-07 1989-04-26 Kowa Company, Ltd. Monoclonal antibodies to tissue plasminogen activator derived from human normal cells
WO1986006487A1 (en) 1985-04-22 1986-11-06 Commonwealth Serum Laboratories Commission Method for determining mimotopes
IL80678A (en) * 1985-11-19 1999-05-09 Schering Biotech Corp Human interleukin-4

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HK105996A (en) 1996-06-28
US5041381A (en) 1991-08-20
WO1989003846A2 (en) 1989-05-05
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CN1176391A (zh) 1998-03-18
AU611750B2 (en) 1991-06-20
NZ226692A (en) 1991-06-25
ES2061736T3 (es) 1994-12-16
EP0375743B1 (en) 1993-12-29
IE62491B1 (en) 1995-02-08
IE883213L (en) 1989-04-26
PT88847B (pt) 1993-01-29
CN1032816A (zh) 1989-05-10
DK101890A (da) 1990-04-25
CA1335653C (en) 1995-05-23
JPH03503118A (ja) 1991-07-18
WO1989003846A3 (en) 1989-05-18
DK101890D0 (da) 1990-04-25
IL88145A0 (en) 1989-06-30
MY108526A (en) 1996-10-31
EP0314402A2 (en) 1989-05-03
DE3886760T2 (de) 1994-09-08
JPH0698020B2 (ja) 1994-12-07
KR930008112B1 (ko) 1993-08-26
EP0375743A1 (en) 1990-07-04
AU2782789A (en) 1989-05-23
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