KR880004738A - 배형성에 의한 해바라기의 재생방법 - Google Patents
배형성에 의한 해바라기의 재생방법 Download PDFInfo
- Publication number
- KR880004738A KR880004738A KR870012059A KR870012059A KR880004738A KR 880004738 A KR880004738 A KR 880004738A KR 870012059 A KR870012059 A KR 870012059A KR 870012059 A KR870012059 A KR 870012059A KR 880004738 A KR880004738 A KR 880004738A
- Authority
- KR
- South Korea
- Prior art keywords
- medium
- regenerating
- sunflower
- plant
- sunflower according
- Prior art date
Links
- 241000208818 Helianthus Species 0.000 title claims abstract 19
- 235000003222 Helianthus annuus Nutrition 0.000 title claims abstract 18
- 230000008929 regeneration Effects 0.000 title abstract 2
- 238000011069 regeneration method Methods 0.000 title abstract 2
- 230000013020 embryo development Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 17
- 239000005556 hormone Substances 0.000 claims abstract 6
- 229940088597 hormone Drugs 0.000 claims abstract 6
- 238000012258 culturing Methods 0.000 claims abstract 4
- 210000002257 embryonic structure Anatomy 0.000 claims abstract 3
- 230000015572 biosynthetic process Effects 0.000 claims abstract 2
- 230000000408 embryogenic effect Effects 0.000 claims abstract 2
- 239000002609 medium Substances 0.000 claims 16
- 230000001172 regenerating effect Effects 0.000 claims 16
- 241000196324 Embryophyta Species 0.000 claims 5
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims 3
- 210000001161 mammalian embryo Anatomy 0.000 claims 3
- 239000011782 vitamin Substances 0.000 claims 3
- 229940088594 vitamin Drugs 0.000 claims 3
- 229930003231 vitamin Natural products 0.000 claims 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 230000030118 somatic embryogenesis Effects 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 235000013343 vitamin Nutrition 0.000 claims 2
- VDRACTHWBNITQP-UHFFFAOYSA-N 2-(3-methylbutyl)-7h-purin-6-amine Chemical compound CC(C)CCC1=NC(N)=C2NC=NC2=N1 VDRACTHWBNITQP-UHFFFAOYSA-N 0.000 claims 1
- 244000020551 Helianthus annuus Species 0.000 claims 1
- 206010020649 Hyperkeratosis Diseases 0.000 claims 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims 1
- 235000005550 amino acid supplement Nutrition 0.000 claims 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims 1
- 239000004062 cytokinin Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000012010 growth Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims 1
- 229960001669 kinetin Drugs 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 239000012882 rooting medium Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 230000009576 somatic growth Effects 0.000 claims 1
- 230000001502 supplementing effect Effects 0.000 claims 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 235000019195 vitamin supplement Nutrition 0.000 claims 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
- A01H6/1464—Helianthus annuus [sunflower]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- General Induction Heating (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Claims (17)
- 이하의 단계로 연속적으로 구성되어 있으며, 세포 또는 조직으로부터의 체세포 배형성에 의해서 헬리안 투스 안누우스 해바라기 재배품종을 재생시키는 방법 : -배형성 캘러스 및 체세포 배형성 및 상기 배의 성장을 위한, 호르몬 함유 영양유도 배지내에서 해바라기 조직을 배양하는 제1단계 : -소위 모종 성장 배지상에서, 상기 배의 발아 및 아마도 뿌리가 내린다면, 식물로 발육되도록 배양하는 제2단계 : -적당하다면, 소위 발근 배지상에서, 상기 식물뿌리의 성장 또는 형성을 위한 제3단계 : 상기 단계들 중, 첫번째의 두 단계는 동일한 성질의 배지상에 수행되며 상기의 배지에는 옥신게 호르몬이 없는 시토키닌계 호르몬의 유효량이 함유되어 있다.
- 제1항에 있어서, 상기 조직이 미성숙 배인 해바라기의 재생방법.
- 제1 및 제2항에 있어서, 상기 호르몬이 키네틴, 6-벤질아미노푸린, 2-이소펜틸아데닌 및 제아틴으로 구성된 군으로 부터 선택된 해바라기의 재생방법.
- 제3항에 있어서, 상기 호르몬이 제1 및 제2 배지 내에서 0.1 내지 8μM 농도의 6-벤질아미노푸린인 해바라기의 재생방법.
- 제1항에 있어서, 상기 6-벤질아미노푸린의 농도가 제1배지에서는 2.2~4.4μM이고, 제2배지에서는 0.44~4.4μM인 해바라기의 재생방법.
- 제1항에 있어서, 상기 첫번째의 두 배지에 있어 제1배지에서는 슈크로오스의 농도가 8%~12%이고, 제2배지에서는 2%~4%인 해바라기의 재생방법.
- 제6항에 있어서, 상기 슈크로오스의 농도가 제1배지에서는 2%~4%인 해바라기의 재생방법.
- 제1항에 있어서, 상기 제1배지로서 무라시지 및 스쿠그(MS)계 배지를 사용하는 해바라기의 재생방법.
- 제1항에 있어서, 상기 제1배지로서 비타민 및 아미노산 보충물을 함유한 무라시지 및 스쿠그(MS)계 배지를 사용하는 해바라기의 재생방법.
- 제1항에 있어서, 상기 제2배지에 비타민 및 아미노산류가 보충되지 않은 제1배지에서와 동일한 양으로 무기염류, 비타민류 및 아미노산이 함유되어 있는 해바라기의 재생방법.
- 제1항에 있어서, 크기가 0.1과 5㎜ 사이이고, 4 내지 21일된 미성숙 배를 배양하는 방법으로서, 상기 배를 재생시킬 수 있는 여러 단편으로 절단할 수 있는 해바라기의 재생방법.
- 제1항에 있어서, 재배품종 T 76, RT 26, HA 89, HA 290, HA 291, HA 300, HA 303 또는 자이언트 그래이 스트라이프를 사용하는 해바라기의 재생방법.
- 제1항에 있어서, 상기의 제2단계 후, 수득된 식물을 뿌리유도를 위해 배양하는 해바라기의 재생방법.
- 제1항에 있어서, 상기의 제2단계 후, 뿌리를 갖는 식물을 토양으로 옮기고 6,000룩스의 광도와 1일당 15h의 광주기를 갖는 15℃의 기후 챔버 내에서 배양하는 해바라기의 재생방법.
- 제1항에 있어서, 상기의 제2단계 후, 수득된 식물을 접목시키는 해바라기의 재생방법.
- 제1항의 방법에 의해서 생산된 해바라기의 식물.
- 제16항의 해바라기의 식물을 의해서 생산된 종자류.※참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8615299 | 1986-10-30 | ||
FR8615299A FR2605839B1 (fr) | 1986-10-30 | 1986-10-30 | Procede de regeneration de tournesol par embryogenese |
Publications (1)
Publication Number | Publication Date |
---|---|
KR880004738A true KR880004738A (ko) | 1988-06-27 |
Family
ID=9340473
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR870012059A KR880004738A (ko) | 1986-10-30 | 1987-10-30 | 배형성에 의한 해바라기의 재생방법 |
Country Status (26)
Country | Link |
---|---|
US (1) | US5017491A (ko) |
EP (1) | EP0266287B1 (ko) |
JP (1) | JPS63129983A (ko) |
KR (1) | KR880004738A (ko) |
CN (1) | CN1019165B (ko) |
AT (1) | ATE68317T1 (ko) |
AU (1) | AU601774B2 (ko) |
BG (1) | BG48561A3 (ko) |
CA (1) | CA1308255C (ko) |
CS (1) | CS276396B6 (ko) |
DE (1) | DE3773836D1 (ko) |
DK (1) | DK566787A (ko) |
ES (1) | ES2025200B3 (ko) |
FR (1) | FR2605839B1 (ko) |
GR (1) | GR3002902T3 (ko) |
HU (1) | HU200070B (ko) |
IL (1) | IL84231A (ko) |
MA (1) | MA21093A1 (ko) |
NZ (1) | NZ222330A (ko) |
PT (1) | PT86050B (ko) |
RO (1) | RO100219B1 (ko) |
SU (1) | SU1727514A3 (ko) |
TN (1) | TNSN87120A1 (ko) |
TR (1) | TR23032A (ko) |
YU (1) | YU198487A (ko) |
ZA (1) | ZA878095B (ko) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0328424A3 (en) * | 1988-02-12 | 1992-04-29 | Kyowa Hakko Kogyo Kabushiki Kaisha | Process for the production of somatic embryos |
AU646116B2 (en) * | 1990-12-06 | 1994-02-10 | Pioneer Hi-Bred International, Inc. | Sunflower plants having a high frequency of regenerability of fertile plants from isolated protoplasts and method of using same |
JP2000517188A (ja) * | 1996-08-30 | 2000-12-26 | ライフ テクノロジーズ,インコーポレイテッド | 無血清哺乳動物細胞培養培地およびその使用 |
US6034307A (en) * | 1997-12-18 | 2000-03-07 | Pannar Seed Limited | Hybrid sunflower plant and seed pan 9612 |
US6066787A (en) * | 1997-12-18 | 2000-05-23 | Pannar Seed Limited | Hybrid sunflower plant and seed PAN 9501 |
CN101258806B (zh) * | 2007-03-08 | 2010-09-22 | 谭宾 | 一种全年栽培向日葵技术 |
US8901377B2 (en) | 2010-12-03 | 2014-12-02 | E. I. Du Pont De Nemours And Company | Method of sunflower regeneration and transformation using radicle free embryonic axis |
CN102301960A (zh) * | 2011-08-18 | 2012-01-04 | 新疆康地种业科技股份有限公司 | 一种建立康地6号油葵再生体系的方法 |
CN103404439B (zh) * | 2013-08-12 | 2015-11-25 | 南京农业大学 | 一种菊芋不定芽诱导及植株再生的方法 |
KR102282265B1 (ko) * | 2014-01-23 | 2021-07-26 | 닛산 가가쿠 가부시키가이샤 | 배양 배지 조성물의 제조 방법 |
CN107182782B (zh) * | 2017-05-09 | 2019-06-18 | 三瑞农业科技股份有限公司 | 一种加速向日葵世代进程的方法 |
CN113508753A (zh) * | 2021-07-26 | 2021-10-19 | 浙江省农业科学院 | 观赏向日葵快繁与组织培养植株再生方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4038778A (en) * | 1976-09-27 | 1977-08-02 | Gte Laboratories Incorporated | Tissue culture technique for asexual plant reproduction and a medium for use therewith |
US4354327A (en) * | 1979-04-27 | 1982-10-19 | International Paper Company | Tissue culture method for asexual propagation of pine trees and medium for use therewith |
US4552844A (en) * | 1983-06-15 | 1985-11-12 | Stauffer Chemical Company | Plant growth medium |
EP0160390A3 (en) * | 1984-04-16 | 1987-04-08 | Sandoz Ltd. | Embryogenic callus and cell suspension of inbred corn |
US4670391A (en) * | 1984-07-27 | 1987-06-02 | Sungene Technologies Corporation | Sunflower regeneration through embryogenesis and organogenesis |
US4673648A (en) * | 1984-07-27 | 1987-06-16 | Sungene Technologies Corporation | Sunflower regeneration through organogenesis |
US4670392A (en) * | 1984-07-27 | 1987-06-02 | Sungene Technologies Corporation | Sunflower regeneration through embryogenesis |
-
1986
- 1986-10-30 FR FR8615299A patent/FR2605839B1/fr not_active Expired
-
1987
- 1987-10-20 IL IL84231A patent/IL84231A/xx not_active IP Right Cessation
- 1987-10-26 MA MA21334A patent/MA21093A1/fr unknown
- 1987-10-26 TR TR734/87A patent/TR23032A/xx unknown
- 1987-10-28 EP EP87420290A patent/EP0266287B1/fr not_active Expired - Lifetime
- 1987-10-28 AU AU80405/87A patent/AU601774B2/en not_active Ceased
- 1987-10-28 ZA ZA878095A patent/ZA878095B/xx unknown
- 1987-10-28 DE DE8787420290T patent/DE3773836D1/de not_active Expired - Fee Related
- 1987-10-28 CA CA000550484A patent/CA1308255C/en not_active Expired - Lifetime
- 1987-10-28 RO RO13021287A patent/RO100219B1/ro unknown
- 1987-10-28 ES ES87420290T patent/ES2025200B3/es not_active Expired - Lifetime
- 1987-10-28 AT AT87420290T patent/ATE68317T1/de not_active IP Right Cessation
- 1987-10-28 NZ NZ222330A patent/NZ222330A/xx unknown
- 1987-10-29 CS CS877763A patent/CS276396B6/cs unknown
- 1987-10-29 JP JP62274585A patent/JPS63129983A/ja active Pending
- 1987-10-29 SU SU874203619A patent/SU1727514A3/ru active
- 1987-10-29 TN TNTNSN87120A patent/TNSN87120A1/fr unknown
- 1987-10-29 HU HU874878A patent/HU200070B/hu not_active IP Right Cessation
- 1987-10-29 DK DK566787A patent/DK566787A/da not_active Application Discontinuation
- 1987-10-30 CN CN87107542A patent/CN1019165B/zh not_active Expired
- 1987-10-30 KR KR870012059A patent/KR880004738A/ko not_active Application Discontinuation
- 1987-10-30 US US07/115,055 patent/US5017491A/en not_active Expired - Fee Related
- 1987-10-30 BG BG081606A patent/BG48561A3/xx unknown
- 1987-10-30 PT PT86050A patent/PT86050B/pt not_active IP Right Cessation
- 1987-10-31 YU YU01984/87A patent/YU198487A/xx unknown
-
1991
- 1991-10-17 GR GR91400652T patent/GR3002902T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
JPS63129983A (ja) | 1988-06-02 |
IL84231A0 (en) | 1988-03-31 |
DK566787D0 (da) | 1987-10-29 |
US5017491A (en) | 1991-05-21 |
SU1727514A3 (ru) | 1992-04-15 |
TNSN87120A1 (fr) | 1990-01-01 |
MA21093A1 (fr) | 1988-07-01 |
EP0266287B1 (fr) | 1991-10-16 |
CA1308255C (en) | 1992-10-06 |
ATE68317T1 (de) | 1991-11-15 |
IL84231A (en) | 1992-01-15 |
PT86050B (pt) | 1990-08-31 |
DK566787A (da) | 1988-05-01 |
AU8040587A (en) | 1988-05-05 |
GR3002902T3 (en) | 1993-01-25 |
HU200070B (en) | 1990-04-28 |
BG48561A3 (en) | 1991-03-15 |
RO100219B1 (en) | 1992-02-24 |
HUT49034A (en) | 1989-08-28 |
PT86050A (fr) | 1987-11-01 |
EP0266287A1 (fr) | 1988-05-04 |
NZ222330A (en) | 1990-08-28 |
ES2025200B3 (es) | 1992-03-16 |
CN1019165B (zh) | 1992-11-25 |
ZA878095B (en) | 1988-04-26 |
FR2605839B1 (fr) | 1989-02-24 |
YU198487A (en) | 1991-10-31 |
CN87107542A (zh) | 1988-06-29 |
DE3773836D1 (de) | 1991-11-21 |
AU601774B2 (en) | 1990-09-20 |
FR2605839A1 (fr) | 1988-05-06 |
CS276396B6 (en) | 1992-05-13 |
TR23032A (tr) | 1989-02-10 |
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