KR880003135Y1 - Multi-distribution device - Google Patents
Multi-distribution device Download PDFInfo
- Publication number
- KR880003135Y1 KR880003135Y1 KR2019850011932U KR850011932U KR880003135Y1 KR 880003135 Y1 KR880003135 Y1 KR 880003135Y1 KR 2019850011932 U KR2019850011932 U KR 2019850011932U KR 850011932 U KR850011932 U KR 850011932U KR 880003135 Y1 KR880003135 Y1 KR 880003135Y1
- Authority
- KR
- South Korea
- Prior art keywords
- dispenser
- culture
- plate
- wells
- hose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000004113 cell culture Methods 0.000 description 6
- 239000012531 culture fluid Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
내용 없음.No content.
Description
제1도는 본 고안의 전체 사시도.1 is an overall perspective view of the present invention.
제2도는 본 고안의 배양액 흡입사시도.2 is a perspective view of the culture fluid inhalation of the present invention.
제3도는 본 고안의 배양액 주입사시도.3 is a perspective view of a culture solution injection of the present invention.
제4(a)도는 제2도의 A-A선 단면도, 제4(b)도는 제3도의 B-B선 단면도이다.4 (a) is a cross-sectional view taken along the line A-A of FIG. 2, and FIG. 4 (b) is a cross-sectional view taken along the line B-B of FIG.
* 도면의 주요부분에 대한 부호의 설명* Explanation of symbols for main parts of the drawings
1 : 분주기판 2 : 디스펜서1: dispenser 2: dispenser
2' : 모세관 3 : 연결구2 ': capillary tube 3: connector
4 : 호오스 5 : 분배구4: hose 5: distribution port
6 : 호오스 7 : 공기펌프6: hose 7: air pump
11 : 배양판 11' : 웰11: culture plate 11 ': well
본 고안은 세포 배양이나 각종 모노클 항체의 생산, 실험 등에 있어서 웰의 배양액에 손상이나 감염을 주지 않고 조작할 수 있는 다중 배분장치(Multi Dispenser)에 관한 것이다.The present invention relates to a multi-dispenser that can be operated without damaging or infecting a culture medium of a well in cell culture, production of various monoclonal antibodies, and experiments.
종래에는 파이팻이나 다공 파이팻 등을 사용하였으나 공기의 힘을 빌어 흡입하거나 토출하게 되어 있어서 힘을 주는 정도에 따라서 흡입량이 다르고 여러번 조작함에 따른 세균감염 등의 문제가 있어 왔다.Conventionally, pipettes and porous pipettes are used, but inhalation or discharge is performed by the force of air, and thus the amount of inhalation varies depending on the degree of force, and there have been problems such as bacterial infection due to multiple manipulations.
본 고안은 상기와 같은 난점을 보완하기 위하여 모세관 현상에 의해 일정량이 흡입되도록 한 다수의 디스펜서를 일정간격으로 배열하여 분주기판에 삽입부착하여 배양액 용기로부터 흡입하여 바둑판처럼 다수의 웰이 형성된 세포 배양판에 주입시켜 주도록 안출한 것으로 인공적 힘을 전혀 가하지 않음으로서 세포에 불필요한 자극을 주지 않고 극히 안정되게 배양액을 수거할 수 있어 배양되는 세포가 분비하는 매량의 각종 인자를 확인 실험할 수 있으며, 동시에 잡균오염 기회를 줄일 수 있는 잇점이 있다. 이를 첨부도면에 의거 상세히 설명하면 다음과 같다.The present invention is a cell culture plate formed with a plurality of wells, such as a checker board by integrating a plurality of dispensers arranged at a predetermined interval to a certain interval by a capillary phenomenon in order to supplement the difficulty as described above, attached to the dispenser and sucked from the culture vessel It is designed to be injected into the body, and by applying no artificial force at all, the culture medium can be collected very stably without unnecessary stimulation to the cells, so that various factors secreted by the cultured cells can be identified and tested. There are advantages to reducing opportunities. This will be described in detail based on the accompanying drawings.
분주기판(1)에 일열로 동일간격으로 다수의 구멍(1')을 형성하고 그 하부에 연결구(3)를 삽입고정하고 그 연결구(3) 하부에는 디스펜서(2)를 삽입부착하고 디스펜서(2)의 내부중심에 모세관(2')을 형성하며 연결구(3) 상부에는 호오스(4)로서 연결한다. 또 분주기판(1)의 후면에 턱(1'')을 형성하여 손잡이 역할을 하도록 한다.A plurality of holes 1 'are formed in the dispenser board 1 at equal intervals, and the connector 3 is inserted into the lower part of the dispenser plate 1, and the dispenser 2 is inserted into and attached to the lower part of the connector 3, and the dispenser 2 ) Form a capillary tube (2 ') in the inner center of the connector (3) is connected to the upper portion as a hose (4). In addition, by forming a jaw (1 '') on the rear of the divider board (1) to serve as a handle.
분주기판(1)에서 나온 다수의 호오스(4)는 분배구(5)에 연결시켜 호오스(6)으로 단일화 시킨후 공기펌프(7)의 토출구와 연결시킨다.A plurality of hoses (4) from the distributor plate (1) is connected to the distribution port (5) to be unified into the hose (6) and then connected to the outlet of the air pump (7).
실험대(8)는 이단으로 형성하고 낮은 하단(8'')에는 공기펌프(7)를 고정구(9)로서 고정시키고 분배구(5)를 놓으며 상단(8')에는 가이드 샤시(10)를 결합시켜 수개의 층을 형성토록 하여 각 가이드 샤시(10)의 서로 내항되는 가장자리(10')에 다수의 웰(11')이 바둑판처럼 형성된 배양판(11)을 놓을 수 있도록 한다. 미설명부호 7'는 공기펌프(7)를 작동시키는 푸쉬버튼이며, 12는 세포배양용기이다.The bench 8 is formed in two stages, and the air pump 7 is fixed as the fixture 9 at the lower bottom 8 ″, the distributor 5 is placed, and the guide chassis 10 is coupled to the top 8 ′. In order to form several layers, a plurality of wells 11 'may be placed on the edges 10' of each guide chassis 10 to place the culture plate 11 formed like a checkerboard. Reference numeral 7 'is a push button for operating the air pump 7, 12 is a cell culture vessel.
이와같이 고안된 본 고안은 다수의 디스펜서(2)가 일열로 배열 부착된 분주기판(1)의 후면 턱(1'')을 잡고 배양액 용기(12)에 살짝 넣고 세포 배양액과 접촉시키면 디스펜서(2)의 내부 중심에 형성된 모세관(2')에 의하여 세포 배양액이 일정량 흡입되어진다.The present invention devised in this manner is to grasp the rear jaw (1 '') of the dispenser plate (1) in which a plurality of dispensers (2) are arranged in a row, gently put it in the culture vessel (12) and contact with the cell culture medium of the dispenser (2) A certain amount of cell culture fluid is aspirated by the capillary 2 'formed at the inner center.
다음 분주판(1)의 후면턱(1'')을 잡고 실험대(8)의 상단(8')에 놓여진 배양판(11)에 다수 형성된 웰(11')에 주입시킨다. 이때 디스펜서(2)의 모세관(2')에 흡입된 배양액은 공기펌프(7)를 푸쉬버튼(7')에 의해 작동시켜 호오스(6), 분배구(5)를 통해서 각 호오스(4)로 공기를 송출하여 디스펜서(2)의 모세관(2')에 도달되어 그 압으로 배양액을 웰(11')에 토출 주입하게 된다. 배양판(11)에 형성된 웰(11')의 일열의 개수와 분주기판(1)의 디스펜서(2)의 일열의 개수와는 동일하게 하여 분주기판(1)의 일회 이동시 일열의 웰(11')에 동시에 주입작업을 시행토록 하여 배양판(11)의 열수와 동일회수 만큼 작업하면 끝나게 되어 있어 세포 배양 실험에 있어서 한꺼번에 다량의 별개군락을 배양해야 하는 경우에 간편하게 이용할 수 있고 특히 한 실험조작에 있어서 배양판(11)을 3장 이상 사용되는데 각 개별 웰(11')에서 배양되는 세포의 배지를 자주 주입할 필요가 있을 시에 본 고안을 이용함으로서 시간적인 단축 및 공기내 잡균의 오염기회를 줄일 수 있어, 세포 배양 특히 세포융합을 행하는 실험에 있어서 세포 배양액의 주입 및 흡입을 용이하게 하여 유전공학, 면역할 분야에 있어서 항체 생산, 실험에 훨씬 간편하고 그밖에 세포 클로닝, 각종 혈청 임상병리 실험 등 광범위하게 효과적으로 사용할 수 있는 특징을 가진다.Next, the rear jaw 1 ″ of the distribution plate 1 is grasped and injected into the wells 11 ′ formed on the culture plate 11 placed on the upper end 8 ′ of the lab table 8. At this time, the culture fluid sucked into the capillary 2 'of the dispenser 2 is operated by the push button 7' by operating the air pump 7 to the hoses 6 and through the distribution port 5 to each hose 4. Air is sent out to reach the capillary 2 'of the dispenser 2, and the pressure is discharged and injected into the well 11' by the pressure. The number of wells 11 'of the wells 11' formed in the culture plate 11 and the number of rows of the dispenser 2 of the distributor plate 1 are equal to each other so that the one row of wells 11 'may be moved once. When the same number of times as the number of hot water of the culture plate (11) to be carried out at the same time to be carried out at the same time to be carried out at the same time can be easily used in the case of culturing a large number of separate colonies in a cell culture experiment, especially in one experimental operation In this case, at least three culture plates 11 are used. When the medium of cells cultured in each individual well 11 'needs to be frequently injected, the present invention can be used to reduce the time and the chance of contaminants in the air. It is possible to reduce and to facilitate the injection and inhalation of cell culture fluids in cell culture experiments, especially cell fusion experiments, which is much more convenient for antibody production and experiments in genetic engineering and immunization fields. , Has a characteristic that can be widely used effectively, various serum clinical pathology tests.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR2019850011932U KR880003135Y1 (en) | 1985-09-18 | 1985-09-18 | Multi-distribution device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR2019850011932U KR880003135Y1 (en) | 1985-09-18 | 1985-09-18 | Multi-distribution device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR870005263U KR870005263U (en) | 1987-04-22 |
| KR880003135Y1 true KR880003135Y1 (en) | 1988-08-31 |
Family
ID=19245313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR2019850011932U Expired KR880003135Y1 (en) | 1985-09-18 | 1985-09-18 | Multi-distribution device |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR880003135Y1 (en) |
-
1985
- 1985-09-18 KR KR2019850011932U patent/KR880003135Y1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| KR870005263U (en) | 1987-04-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| UA0108 | Application for utility model registration |
Comment text: Application for Utility Model Registration Patent event code: UA01011R08D Patent event date: 19850918 |
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| UA0201 | Request for examination |
Patent event date: 19850918 Patent event code: UA02012R01D Comment text: Request for Examination of Application |
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| UG1501 | Laying open of application | ||
| UG1604 | Publication of application |
Patent event code: UG16041S01I Comment text: Decision on Publication of Application Patent event date: 19880726 |
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| E701 | Decision to grant or registration of patent right | ||
| UE0701 | Decision of registration |
Patent event date: 19881111 Comment text: Decision to Grant Registration Patent event code: UE07011S01D |
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| REGI | Registration of establishment | ||
| UR0701 | Registration of establishment |
Patent event date: 19881208 Patent event code: UR07011E01D Comment text: Registration of Establishment |
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| UR1002 | Payment of registration fee |
Start annual number: 1 End annual number: 3 Payment date: 19881208 |
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| LAPS | Lapse due to unpaid annual fee | ||
| UC1903 | Unpaid annual fee |
Termination date: 19930724 Termination category: Default of registration fee |