[go: up one dir, main page]

KR850001287A - 폴리펩타이드의 제조방법 - Google Patents

폴리펩타이드의 제조방법 Download PDF

Info

Publication number
KR850001287A
KR850001287A KR1019840004448A KR840004448A KR850001287A KR 850001287 A KR850001287 A KR 850001287A KR 1019840004448 A KR1019840004448 A KR 1019840004448A KR 840004448 A KR840004448 A KR 840004448A KR 850001287 A KR850001287 A KR 850001287A
Authority
KR
South Korea
Prior art keywords
polypeptide
residue
formula
genetic engineering
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1019840004448A
Other languages
English (en)
Inventor
엔겔스 오아힘 (외 4)
Original Assignee
하인리히 벡커,베른하르트 벡크
훽스트 아크티엔 게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 하인리히 벡커,베른하르트 벡크, 훽스트 아크티엔 게젤샤프트 filed Critical 하인리히 벡커,베른하르트 벡크
Publication of KR850001287A publication Critical patent/KR850001287A/ko
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

내용 없음

Description

폴리펩타이드의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 유전자부분 I을 함유하는 잡종 플라스미드에 관한 도이다.
제2도는 유전자부분 II을 함유하는 잡종 플라스미드에 관한 도이다.
제3도는 발현플라스미드 PWH 1에 관한 도이다.

Claims (4)

  1. 일반식(II)의 폴리펩타이드를 유전공학적 방법으로 생성시키고 이 생성물을 효소적으로 전환시켜 일반식(I)의 폴리펩타이드를 제조하는 방법.
    Y-R-NH2(I)
    Y-R-NH-CH2-COOH (II)
    상기 식에서, Y는 틸티오닌잔기 또는 메티오닌을 통해 결합된 세균성 단백질의 잔기를 나타내고;
    R은 유전적으로 코오드화할 수 있는 아미노산의 펩타이드 서열을 나타낸다.
  2. 제1항에 있어서, R이 메티오닌 잔기를 함유하지 않는 일반식(II)의 폴리펩타이드를 유전공학적 방법으로 생성시킨 다음, 잔기 Y를 시아노겐브로마이드를 사용하여 분리, 제거하는 방법.
  3. 제1항 또는 2항에 있어서, R이 아미노산서열(GRF 1 내지 n)(여기서, GRF는 성장호르몬 방출인자 또는 이 서열의 변형을 나타내고, n은 16 내지 44를 나타낸다)인 방법.
  4. 제1항 내지 3항중 어느 하나에 있어서, 유전공학적 방법으로 일반식(II)의 폴리펩타이드를 생성할 때 2개의 중지코돈을, 글리신을 코드화하는 트리플레트 뒤에다 위치시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840004448A 1983-07-27 1984-07-26 폴리펩타이드의 제조방법 Ceased KR850001287A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19833327007 DE3327007A1 (de) 1983-07-27 1983-07-27 Herstellung von polypeptiden mit einem saeureamid-carboxyterminus
DEP3327007.4 1983-07-27

Publications (1)

Publication Number Publication Date
KR850001287A true KR850001287A (ko) 1985-03-18

Family

ID=6205008

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840004448A Ceased KR850001287A (ko) 1983-07-27 1984-07-26 폴리펩타이드의 제조방법

Country Status (15)

Country Link
US (1) US5457033A (ko)
EP (1) EP0133282B1 (ko)
JP (1) JPH07121229B2 (ko)
KR (1) KR850001287A (ko)
AT (1) ATE68827T1 (ko)
AU (1) AU572171B2 (ko)
CA (1) CA1224166A (ko)
DE (2) DE3327007A1 (ko)
DK (1) DK171940B1 (ko)
ES (1) ES8601303A1 (ko)
GR (1) GR82436B (ko)
IE (1) IE57664B1 (ko)
IL (1) IL72489A0 (ko)
PT (1) PT78979A (ko)
ZA (1) ZA845779B (ko)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61257187A (ja) * 1985-05-11 1986-11-14 Sagami Chem Res Center サケカルシトニン誘導体遺伝子
JPS61291598A (ja) * 1985-06-18 1986-12-22 Sagami Chem Res Center 魚類カルシトニン誘導体及びその製造方法
DE3436819A1 (de) * 1984-10-06 1986-04-17 Hoechst Ag, 6230 Frankfurt Arzneimittel mit grf-wirkung
IT1234977B (it) * 1985-03-22 1992-06-09 Serono Ist Farm Espressione in e. coli polipeptidi ibridi contenenti la sequenza del fattore di rilascio dell'ormone della crescita
FR2587359B1 (fr) * 1985-05-28 1987-12-24 Sanofi Sa Derives non amides de la somatocrinine et procede de preparation par genie genetique
EP0212531A1 (en) * 1985-08-12 1987-03-04 Syntex (U.S.A.) Inc. Bovine growth hormone releasing factor derivative
GB8523156D0 (en) * 1985-09-19 1985-10-23 Celltech Ltd Polypeptide production
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
DE3632037A1 (de) * 1986-09-20 1988-04-07 Hoechst Ag Gentechnisches verfahren zur herstellung von salm-calcitonin sowie mittel zur durchfuehrung dieses verfahrens
US5789234A (en) * 1987-08-14 1998-08-04 Unigene Laboratories, Inc. Expression systems for amidating enzyme
JPH01144981A (ja) * 1987-12-02 1989-06-07 Wakunaga Pharmaceut Co Ltd 蛋白質の製造
EP0490249B1 (en) * 1990-12-10 1995-03-15 F. Hoffmann-La Roche Ag Process for the enzymatic preparation of GRF(1-44)NH2
GB0512610D0 (en) 2005-06-18 2005-07-27 Hexcel Composites Ltd Composite material

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108387B1 (en) * 1982-11-04 1990-05-16 F. Hoffmann-La Roche Ag Preparation of recombinant growth releasing factors
US4581168A (en) * 1983-02-21 1986-04-08 Sanofi Synthesis of hpGRF (Somatocrinin) in liquid phase and intermediate peptides
DE3482387D1 (en) * 1983-03-07 1990-07-05 Hoffmann La Roche Polypeptide.
ZA844380B (en) * 1983-07-05 1985-01-30 Salk Inst For Biological Studi Dna encoding a grf precursor
EP0191869A4 (en) * 1984-08-17 1988-06-27 Sagami Chem Res FISH-CALCITONINE COMBAT AND ITS PRODUCTION AND GENE SYSTEM FOR THIS.

Also Published As

Publication number Publication date
ATE68827T1 (de) 1991-11-15
DK171940B1 (da) 1997-08-18
IE57664B1 (en) 1993-02-24
ZA845779B (en) 1985-02-27
EP0133282A1 (de) 1985-02-20
PT78979A (de) 1984-08-01
EP0133282B1 (de) 1991-10-23
DE3327007A1 (de) 1985-02-07
DK365484A (da) 1985-01-28
ES534589A0 (es) 1985-10-16
JPS6049798A (ja) 1985-03-19
IL72489A0 (en) 1984-11-30
US5457033A (en) 1995-10-10
JPH07121229B2 (ja) 1995-12-25
DE3485199D1 (ko) 1991-11-28
AU3121784A (en) 1985-01-31
AU572171B2 (en) 1988-05-05
DK365484D0 (da) 1984-07-26
CA1224166A (en) 1987-07-14
GR82436B (ko) 1984-12-13
IE841930L (en) 1985-01-27
ES8601303A1 (es) 1985-10-16

Similar Documents

Publication Publication Date Title
KR850001287A (ko) 폴리펩타이드의 제조방법
KR850004273A (ko) 안정성이 증대된 재조합 감마 인터페론 및 그의 제조방법
EP0722461A4 (en) EXPRESSION OF FUSION POLYPEPTIDES TRANSPORTED OUT OF CYTOPLASM WITHOUT HEAD SEQUENCE
KR870005098A (ko) 융합 단백질의 제조방법
AU2656695A (en) Purified scytalidium laccases and nucleic acids encoding same
ATE81779T1 (de) Peptidanalogedes insulinaehnlichen wachstumsfaktors-1 bei saeugetieren.
AU2827895A (en) Purified polyporus laccases and nucleic acids encoding same
DE59611434D1 (de) Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
DK583388D0 (da) Termoplastiske, transparente polyamider og fremgangsmaade til fremstilling deraf
EP0489711A3 (en) Method for producing human growth hormone
KR890000517A (ko) 하이브리드 단백질 c 및 이의 제조방법
DK390689D0 (da) Fremgangsmaade til fremstilling af des-b30-insuliner og des-b30-insulinderivater
KR840009069A (ko) 향정신성 펩티드류의 제조 방법
KR860003832A (ko) 메티오닐 결합에 있어서의 펩티드 및 단백질의 분해방법
ES8504255A1 (es) Procedimiento para la produccion de una hormona de crecimiento de porcino.
DE3482982D1 (de) Herstellung von l-aminosaeuren durch transaminierung.
KR850001775A (ko) 인슐린 유도체의 제조방법
GB1375811A (ko)
KR910016767A (ko) 신규한 펩티드
AU4611589A (en) Enzyme-inhibiting amino acid derivatives, a process for the preparation thereof, agents containing these, and the use thereof.
KR870004139A (ko) 인체 λ-인터페론의 생물학적 활성 유도체를 제조하는 방법
GB8414354D0 (en) Purifying protein
KR850004791A (ko) 프로테아제 억제제의 제조방법
ES8506092A1 (es) Un metodo de producir un precursor del factor de liberacion de la hormona del crecimiento humana
KR890008166A (ko) 융합 단백질의 선택적 분해 방법

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19840726

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19890726

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19840726

Comment text: Patent Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19910701

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 19910919

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 19910701

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I