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KR830000273A - A method of making a delivery medium for cyclic deoxyribonucleic acid (DNA) and a method of transferring genetic factors of higher organisms to microorganisms - Google Patents

A method of making a delivery medium for cyclic deoxyribonucleic acid (DNA) and a method of transferring genetic factors of higher organisms to microorganisms Download PDF

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Publication number
KR830000273A
KR830000273A KR1019780001672A KR780001672A KR830000273A KR 830000273 A KR830000273 A KR 830000273A KR 1019780001672 A KR1019780001672 A KR 1019780001672A KR 780001672 A KR780001672 A KR 780001672A KR 830000273 A KR830000273 A KR 830000273A
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KR
South Korea
Prior art keywords
dna
specific protein
mrna
making
nucleotide
Prior art date
Application number
KR1019780001672A
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Korean (ko)
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KR840001441B1 (en
Inventor
제이. 룻터 윌리암
미차엘 굿만 호워드
울리히 악셀
샤인 죤
미첼 칠그윈 죤
루이스 픽테트 레이몬드
호스트 시버그 페터
Original Assignee
오. 한센
더 리젠스 오브 더 유니버시티 오브 칼리포니아
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Priority to KR7801672A priority Critical patent/KR840001441B1/en
Publication of KR830000273A publication Critical patent/KR830000273A/en
Application granted granted Critical
Publication of KR840001441B1 publication Critical patent/KR840001441B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

조환형(組換型) 디옥시리보핵산(DNA) 전달매개체를 만드는 방법 및 고등생물의 유전인자를 미생물에 전달시키는 방법A method of making a delivery medium for cyclic deoxyribonucleic acid (DNA) and a method of transferring genetic factors of higher organisms to microorganisms

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음This content is subject to disclosure, so the full text is not included.

도면 1은 본 발명의 과정을 도식화한 그림이다.1 is a diagram schematically illustrating the process of the present invention.

Claims (1)

도시하고 본문에 상술한 바와 같이, 특정한 단백질을 생산할 수 있는 고등생물로부터 특정한 단백질에 대한 mRNA 유전부호로 포함하고 있는 세포를 분리하고, mRNA의 RN 아제에 의한 감소를 막기 위하여 RN 아제 억제제 존재하에 분리된 세포로부터 mRNA를 추출하고, 단백질, DNA 및 기타 DNA가 거의 없는 mRNA를 분리하고, 한가닥이 mRNA에 대응하는 뉴클레오티드 결합체를 가진 이중가닥 cDNA를 합성하여 특정한 단백질에 대한 뉴클레오티드 결합체를 가진 cDNA를 생성하고, 이중가닥 cDNA와 연결할 수 있는 활성잔기를 가진 DNA 변형매개체를 가하고, 특정한 단백질에 대한 뉴클레오티드 유전부호 결합체를 가진 이중가닥 cDNA와 DNA 변형 매개체를 연결시켜 특정한 단백질에 대한 뉴클레오티드 유전부호 결합체를 가진 DNA 변형매개체를 형성함으로써 고등생물의 특정한 단백질에 대한 뉴클레오티드 유전부호 결합체를 함유하는 DNA 변형매개체를 만드는 방법.As shown and described above in the text, cells containing the mRNA genetic code for a specific protein are isolated from higher organisms capable of producing a specific protein, and isolated in the presence of an RNase inhibitor to prevent the reduction of mRNA by RNase. MRNA is extracted from the cells, protein, DNA, and other DNA-free mRNA is isolated, and double-stranded cDNA having a nucleotide conjugate corresponding to the mRNA is synthesized to generate cDNA having a nucleotide conjugate for a specific protein. , DNA modification with a nucleotide genetic code conjugate for a specific protein by adding a DNA modification medium having an active residue capable of linking with a double-stranded cDNA and linking the double-stranded cDNA with a nucleotide genetic code conjugate for a specific protein and the DNA modification medium A method of making a DNA modification medium containing a nucleotide genetic code conjugate for a specific protein of a higher organism by forming a medium. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed according to the contents of the initial application.
KR7801672A 1978-06-01 1978-06-01 Recombinant dna transfer vector containing a gene from a higher organism KR840001441B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR7801672A KR840001441B1 (en) 1978-06-01 1978-06-01 Recombinant dna transfer vector containing a gene from a higher organism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR7801672A KR840001441B1 (en) 1978-06-01 1978-06-01 Recombinant dna transfer vector containing a gene from a higher organism

Publications (2)

Publication Number Publication Date
KR830000273A true KR830000273A (en) 1983-03-30
KR840001441B1 KR840001441B1 (en) 1984-09-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR7801672A KR840001441B1 (en) 1978-06-01 1978-06-01 Recombinant dna transfer vector containing a gene from a higher organism

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Publication number Publication date
KR840001441B1 (en) 1984-09-27

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