KR20250006404A - A water-in-oil cosmetic composition comprising cell culture medium and albumin and use thereof - Google Patents

Abstract

The present invention relates to a water-in-oil type cosmetic composition for skin improvement containing a cell culture medium and albumin. The cosmetic composition of the present invention provides stability of the formulation while containing a cell culture medium and albumin, and thus can be usefully used as a water-in-oil type cosmetic composition for skin improvement that can be smoothly spread and provides a feeling of nutrition without being sticky after absorption.

Description

{A water-in-oil cosmetic composition comprising cell culture medium and albumin and use thereof}

The present invention relates to a water-in-oil type cosmetic composition containing a cell culture medium and albumin and its use.

The cells that make up skin cells are a type of animal cell, and representative examples are keratinocytes and fibroblasts. The general composition of the medium for culturing the above cells includes DMEM (Dulbecco's Modified Eagle's Medium) and fetal bovine serum (FBS).

Serum is known to play a role in promoting growth, providing nutrients, and protecting cells from oxidation and toxins. When serum is excluded from cell culture, cell culture itself is hardly performed, so serum is recognized as an essential component for cell growth and proliferation. However, it has been recently reported that FBS can cause the outbreak of human mad cow disease. This can threaten the safety of the tester, and since serum is collected directly from fetal bovine fetuses, it can also be a problem in terms of animal protection. In addition, FBS is expensive, so it has the disadvantage of increasing the price of the medium.

Accordingly, the prior art (Korean Patent No. 10-2368465) discloses a composition that does not contain FBS. However, since the composition mainly consists of salts and amino acids, there is a problem that it affects the stability of the formulation and affects the interface of the water-in-oil type formulation, causing particle breakage or lowering of viscosity.

Under these circumstances, a technique capable of improving the stability of the formulation of the composition was developed, thereby completing the present invention.

Republic of Korea Patent No. 10-2368465

One aspect provides a water-in-oil type cosmetic composition containing a cell culture medium and albumin as active ingredients while ensuring stability of the formulation.

Another aspect provides a water-in-oil type composition for external skin application containing a cell culture medium and albumin as active ingredients while ensuring stability of the formulation.

One aspect is to provide a water-in-oil (W/O) cosmetic composition comprising a cell culture medium and albumin.

The term “cell culture medium” in this specification contains components required by cells for cell growth and survival in vitro, or components that help cell growth and survival. Specifically, the components may include vitamins, essential or non-essential amino acids, and trace elements. The medium may be a medium used for culturing cells, preferably eukaryotic cells, and more preferably animal cells.

Examples of the above cell culture media include, but are not limited to, DMEM/F-12 (Dulbecco's Modified Eagle’s Medium/Nutrient Mixture F-12), DMEM, MEMα (Minimum Essential Mediumα), BME (Basal Medium Eagle), GMEM (Glasgow Minimum Essential Medium), RPMI-1640 Medium, etc.

In addition, the cell culture medium of the present invention is a serum-free medium. A serum-free medium means a culture medium that does not contain serum derived from animals including humans (e.g., animal-derived serum). Animal-derived serum provides general nutrients for the growth of cells to be cultured, but contains unidentified trace components, making analysis difficult, making it difficult to establish reproducible testing and production processes, and having problems in that safety to the human body cannot be guaranteed. However, the cell culture medium according to the present invention is a serum-free medium, so safety to the human body is guaranteed, and reproducible testing and production processes can be established.

In addition, the above cell culture medium refers to a cell culture medium before culturing cells. Since it is a culture medium before culturing cells, there is no change in the components of the cell culture medium, so it can be used commercially with a uniform quality.

In one embodiment, the cell culture medium may include at least one selected from the group consisting of amino acid components, vitamin components, mineral salt components, and other components, and may additionally include purified water.

The above amino acid component comprises at least one amino acid selected from the group consisting of glycine, L-alanine, L-valine, L-leucine, L-isoleucine, L-threonine, L-serine, L-cysteine, L-methionine, L-aspartic acid, L-asparagine, L-glutamic acid, L-glutamine, L-lysine, L-arginine, L-histidine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, β-alanine, γ-aminobutyric acid, ornithine, citrulline, homoserine, triiodotyrosine, thyroxine and deoxyphenylalanine, and preferably, glycine, L-alanine, L-arginine, L-cysteine, L-glutamine, L-histidine, L-lysine, L-methionine, L-proline, It may comprise one or more amino acids selected from the group consisting of L-serine, L-threonine and L-valine.

The above vitamin component may include at least one vitamin selected from the group consisting of biotin, D-calcium pantothenate, folic acid, niacinamide, pyridoxine hydrochloride, riboflavin, thiamine hydrochloride, vitamin B12, choline chloride, i-inositol and ascorbic acid, and preferably, at least one vitamin selected from the group consisting of i-inositol, thiamine hydrochloride, niacinamide and pyridoxine hydrochloride.

The above inorganic salt component includes at least one inorganic salt selected from the group consisting of calcium chloride (CaCl ₂ ) (anhydrous), copper sulfate pentahydrate (CuSO ₄ -5H 2 O), ferric sulfate heptahydrate (FeSO 4 -7H ₂ O), magnesium chloride (anhydrous), magnesium sulfate (MgSO ₄ ) (anhydrous), potassium chloride (KCl), sodium chloride (NaCl), disodium hydrogen phosphate (Na ₂ HPO ₄ ), sodium hydrogen phosphate monohydrate (NaH ₂ PO ₄ -H ₂ O), zinc sulfate heptahydrate (ZnSO ₄ -7H ₂ O), ferric nitrate nonahydrate (Fe(NO ₃ ) ₃ 9H ₂ O), and sodium bicarbonate (NaHCO ₃ ), preferably, sodium chloride (NaCl), sodium bicarbonate (NaHCO ₃ ), potassium chloride (KCl), It may contain one or more inorganic salts selected from the group consisting of calcium chloride (CaCl ₂ ) (anhydrous) and sodium hydrogen phosphate monohydrate (NaH ₂ PO ₄ -H ₂ O).

The above other components may include at least one other component selected from the group consisting of D-glucose (dextrose), sodium pyruvate, hypoxanthine Na, thymidine, linoleic acid, lipoic acid, adenosine, cytidine, guanosine, uridine, 2'-deoxyadenosine, 2'-deoxycytidine HCl and 2'-deoxyguanosine, and preferably, may include sodium pyruvate.

The purified water is used to dissolve the amino acids, vitamins, mineral salts and other ingredients, and may be water obtained through one or more distillations or purified through a filter.

In one embodiment, the cell culture medium may include glycine, L-alanine, L-arginine hydrochloride, L-cysteine hydrochloride-monohydrate, L-glutamine, L-histidine hydrochloride-monohydrate, L-lysine hydrochloride, L-methionine, L-proline, L-serine, L-threonine, L-valine, i-inositol, thiamine hydrochloride, niacinamide, pyridoxine hydrochloride, sodium chloride (NaCl), sodium bicarbonate (NaHCO ₃ ), potassium chloride (KCl), calcium chloride (CaCl ₂ ) (anhydrous), sodium bicarbonate monohydrate (NaH ₂ PO ₄ -H ₂ O), and sodium pyruvate, and may be specifically composed of the above components.

The above amino acid component is used for cell growth as a raw material for protein synthesis, and it includes 0.2 to 1.2 parts by weight of glycine, 0.08 to 0.3 parts by weight of L-alanine, 2 to 7 parts by weight of L-arginine hydrochloride, 0.2 to 0.9 parts by weight of L-cysteine hydrochloride-monohydrate, 10 to 18 parts by weight of L-glutamine, 0.8 to 2.0 parts by weight of L-histidine hydrochloride-monohydrate, 1.0 to 5.0 parts by weight of L-lysine hydrochloride, 0.2 to 1.2 parts by weight of L-methionine, 0.2 to 1.2 parts by weight of L-proline, 1.0 to 5.0 parts by weight of L-threonine, and 1.0 to 5.0 parts by weight of L-valine, based on 1 part by weight of serine, an amino acid contained in a cell culture medium. Can be.

In one embodiment, the amino acid component and content included in the cell culture medium may be 0.5 to 1.0 parts by weight of glycine, 0.10 to 0.02 parts by weight of L-alanine, 5.0 to 6.0 parts by weight of L-arginine hydrochloride, 0.4 to 0.7 parts by weight of L-cysteine hydrochloride-monohydrate, 12 to 15 parts by weight of L-glutamine, 1.0 to 1.5 parts by weight of L-histidine hydrochloride-monohydrate, 2.0 to 4.0 parts by weight of L-lysine hydrochloride, 0.4 to 0.8 parts by weight of L-methionine, 0.4 to 0.8 parts by weight of L-proline, 1.5 to 2.5 parts by weight of L-threonine, and 1.5 to 2.5 parts by weight of L-valine. By including amino acid components in a content within the above range, it is possible to help cell growth and maintenance and improve formulation stability.

In the above cell culture medium, the vitamin component plays a role in maintaining cell activity and may include 0.2 to 0.9 parts by weight of i-inositol, 0.04 to 0.8 parts by weight of thiamine hydrochloride, 0.04 to 0.8 parts by weight of niacinamide, and 0.04 to 0.8 parts by weight of pyridoxine hydrochloride based on 1 part by weight of amino acid serine.

In one embodiment, the vitamin component and content included in the cell culture medium may be 0.4 to 0.7 parts by weight of i-inositol, 0.06 to 0.2 parts by weight of thiamine hydrochloride, 0.06 to 0.2 parts by weight of niacinamide, and 0.06 to 0.2 parts by weight of pyridoxine hydrochloride based on 1 part by weight of amino acid serine. By including the vitamin component in an amount within the above range, it may help maintain cell activity.

The above-mentioned inorganic salt component plays a role in regulating the expression of cell function, and may include 50 to 200 parts by weight of sodium chloride (NaCl), 50 to 120 parts by weight of sodium bicarbonate (NaHCO ₃ ), 2.0 to 20 parts by weight of potassium chloride (KCl), 1.0 to 10 parts by weight of calcium chloride (CaCl ₂ ) (anhydrous), and 0.5 to 5.0 parts by weight of sodium biphosphate monohydrate (NaH ₂ PO ₄ -H ₂ O) based on 1 part by weight of amino acid serine.

In one embodiment, the inorganic salt component and content included in the cell culture medium may be 100 to 150 parts by weight of sodium chloride (NaCl), 80 to 100 parts by weight of sodium bicarbonate (NaHCO ₃ ), 8 to 15 parts by weight of potassium chloride (KCl), 2.0 to 8.0 parts by weight of calcium chloride (CaCl ₂ ) (anhydrous), and 1.0 to 4.0 parts by weight of sodium biphosphate monohydrate (NaH ₂ PO ₄ -H ₂ O) based on 1 part by weight of amino acid serine.

The above other components may include components that can improve the stability of the cell culture medium, etc., and may include 0.5 to 5.0 parts by weight of sodium pyruvate based on 1 part by weight of amino acid serine.

In one embodiment, the other components and content included in the cell culture medium may be 1.0 to 4.0 parts by weight of sodium pyruvate based on 1 part by weight of amino acid serine. Other components included in the content within the above range may help improve formulation stability, etc.

The albumin included in the above composition may be human serum albumin or bovine serum albumin.

The above human serum albumin is serum albumin found in human blood and is the most abundant protein present in human plasma. In the present invention, the human serum albumin may specifically be recombinant human serum albumin. In addition, the above bovine serum albumin is serum albumin found in cattle and is often used as a protein concentration standard in experiments.

The above albumin content may be contained in a concentration of 10 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 300 μg/ml, 500 μg/ml, 1000 μg/ml. Alternatively, the albumin content may be contained in a concentration of 10 to 1000 μg/ml, 50 to 500 μg/ml, 100 to 250 μg/ml.

The above cell culture medium and albumin may be present in an amount of 0.0001 to 10 wt% based on the total weight of the composition, and specifically, 0.0001 to 10 wt%, 0.0001 to 5 wt%, 0.0001 to 3 wt%, 0.0001 to 2 wt%, 0.0001 to 1 wt%, 0.001 to 10 wt%, 0.001 to 5 wt%, 0.001 to 3 wt%, 0.001 to 2 wt%, 0.001 to 1 wt%, 0.01 to 10 wt%, 0.01 to 5 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.1 to It may be present in an amount of 3 wt%, 0.1 to 2 wt%, 0.1 to 1 wt%, 0.25 to 10 wt%, 0.25 to 5 wt%, 0.25 to 3 wt%, 0.25 to 2 wt%, 0.25 to 1 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, 0.5 to 1 wt%, 0.75 to 10 wt%, 0.75 to 5 wt%, 0.75 to 3 wt%, 0.75 to 2 wt%, or 0.75 to 1 wt%.

The above cosmetic composition may be for improving skin condition.

The above skin condition improvement may include at least one selected from the group consisting of skin moisturizing, skin aging improvement, skin barrier strengthening, skin wrinkle improvement, skin elasticity enhancement, skin texture improvement, skin transparency enhancement, skin blemish and freckle removal, skin whitening, and skin regeneration.

The term "skin moisturizing" as used herein may mean any action that maintains skin moisture or prevents moisture loss.

The term "skin aging" as used herein refers to a general term for tangible and intangible changes that occur in the skin with age, such as a thinning of the epidermis, a decrease in the number of cells or blood vessels in the dermis, DNA damage repair ability, cell turnover cycle, wound healing, skin barrier function, moisture retention in the epidermis, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical removal ability, immune response, sensory function, and temperature regulation.

The above skin aging improvement may mean skin aging improvement caused by exogenous factors or endogenous factors. The exogenous factors refer to various external factors, such as ultraviolet rays (light), and the endogenous factors are also referred to as chronological factors and mainly refer to factors that occur due to the passage of time. That is, the skin aging includes not only premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, and chemicals, but also natural aging phenomena that occur as skin cell proliferation decreases with age, and is a concept that includes wrinkles, loss of elasticity, sagging skin, and dryness. In addition, wrinkles include stimuli caused by changes in internal and external factors that change the components that make up skin tissue, thereby causing wrinkles.

The above aging may be photoaging. The above term "photoaging" is a phenomenon caused by external environmental factors, the most representative of which is ultraviolet rays. Ultraviolet rays cause damage to biological components such as activation of proteolytic enzymes, chain cleavage of substrate proteins, and abnormal cross-linking, and repetition of this mechanism causes skin aging that is evident in appearance.

The term "strengthening skin barrier" in this specification may mean any action that enhances the function of the skin barrier, which is located at the outermost part of the skin and prevents moisture and nutrient loss.

The term "wrinkle" in this specification means a state in which skin loses elasticity and becomes loose, and may be, for example, skin folding. The above "prevention or improvement of skin wrinkles" may mean any action that prevents or improves wrinkles by suppressing the expression of factors related to wrinkles, or increases the total amount of collagen.

The term "skin transparency" in this specification means the ratio of transmitted light to incident light at the skin surface. After some of the incident light is scattered and dispersed at the skin surface, the remainder is transmitted into the skin, and the transmitted light (transmitted light) is reflected back out from inside the skin. As the amount of light reflected from inside the skin (diffuse reflection) increases, skin transparency increases.

The term "improving skin elasticity" as used herein means alleviating the degree of sagging or loosening of the skin.

The term "skin microrelief" in this specification refers to a three-dimensional microstructure formed by fine lines on the skin surface. It is an indicator of skin condition separately from elasticity, wrinkles, and moisture, and more specifically, skin texture is a reliable indicator of aging that can detect aging before aging begins. In addition, skin texture is not simply a rough and smooth skin surface condition, but rather reflects the condition inside the skin, and the skin texture of the visible skin surface is only a part of the entire skin structure that is revealed outside, and damage to the skin texture reflects changes inside the skin.

The term "skin regeneration" in this specification may mean any action that replenishes a portion of the skin that has been lost or promotes the proliferation of skin cells. Specifically, skin regeneration may mean the regeneration of skin tissue at a site of a skin wound.

The term "skin whitening" in this specification may mean not only brightening the skin tone by inhibiting the synthesis of melanin pigment, but also improving skin hyperpigmentation such as blemishes or freckles caused by ultraviolet rays, hormones, or genetics.

The term "freckle" in this specification is thought to be caused by a complex interplay of various factors, such as cloudiness or shade due to thickening of the stratum corneum accompanying aging, decreased redness of the skin, deposition of pigments, decreased elasticity of the skin, yellowing of the skin, and contamination with sweat or sebum.

The above cosmetic composition may have enhanced skin condition improvement efficiency, and specifically, may have enhanced skin condition improvement efficiency compared to a composition that does not contain a cell culture medium and/or albumin as active ingredients.

Specifically, the skin condition improvement efficiency of the cosmetic composition may be enhanced by 10% or more, 30% or more, 50% or more, 70% or more, 100% or more, 150% or more, 200% or more, or 300% or more, compared to a composition that does not contain a cell culture medium and/or albumin as an active ingredient, and specifically, by 10% to 300%, 10% to 200%, 10% to 150%, 10% to 100%, 10% to 70%, 10% to 50%, 30% to 300%, 30% to 200%, 30% to 150%, 30% to 100%, 30% to 70%, 30% to 50%, 50% to 300%, 50% to 200%, It can be enhanced by 50% to 150%, 50% to 100%, or 50% to 70%.

The above-mentioned water-in-oil composition may additionally contain at least one selected from the group consisting of a surfactant, a wax, a butter, a thickener (stabilizer), a higher alcohol, and a higher fatty acid.

In one embodiment, the water-in-oil composition may include a cell culture medium, albumin, a surfactant, a wax, and a thickening agent (stabilizer).

The above surfactants are PEG-8 dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone, PEG-10 dimethicone, PEG-12 dimethicone, PEG-11 methyl ether dimethicone, cetyl PEG/PPG dimethicone, cetyl PEG/PPG-10/1 dimethicone, bis-PEG/PPG-14/14 dimethicone, bis-PEG/PPG-20/20 dimethicone, PEG/PPG-18/18 dimethicone, PEG/PPG-19/19 dimethicone, PEG/PPG-20/20 dimethicone, polyglyceryl-3 polydimethylsiloxyethyl dimethicone, lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone, and lauryl PEG-9. It may include at least one selected from the group consisting of, but is not limited to, polydimethylsiloxyethyl dimethicone.

The surfactant may be present in an amount of 0.01 to 10 wt% based on the total weight of the composition, and specifically, 0.01 to 10 wt%, 0.01 to 5 wt%, 0.01 to 4 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.1 to 4 wt%, 0.1 to 3 wt%, 0.1 to 2 wt%, 0.1 to 1 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 0.5 to 4 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, 0.5 to 1 wt%, 1 to 10 wt%, 1 to 5 wt%, 1 to 4 wt%, 1 to It may be comprised in an amount of 3 wt%, 1 to 2 wt%, 2 to 10 wt%, 2 to 5 wt%, 2 to 4 wt%, 2 to 3 wt%, 3 to 10 wt%, 3 to 5 wt% or 3 to 4 wt%.

The above wax may include at least one selected from the group consisting of beeswax, candelilla wax, carnauba wax, multiwax, paraffin wax, microcrystal wax and ceresin wax, but is not limited thereto.

The wax may be present in an amount of 0.01 to 10 wt% based on the total weight of the composition, and specifically, 0.01 to 10 wt%, 0.01 to 5 wt%, 0.01 to 4 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1.5 wt%, 0.01 to 1 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.1 to 4 wt%, 0.1 to 3 wt%, 0.1 to 2 wt%, 0.1 to 1.5 wt%, 0.1 to 1 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 0.5 to 4 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, 0.5 to 1.5 wt%, 0.5 It may be included at 1 wt%, 1 to 10 wt%, 1 to 5 wt%, 1 to 4 wt%, 1 to 3 wt%, 1 to 2 wt% or 1 to 1.5 wt%.

The above thickener may include at least one selected from the group consisting of hectorite, quaternium-18 hectorite, stearalkonium hectorite, and disteardimonium hectorite, as a viscosity control agent and stabilizer of the continuous phase, but is not limited thereto.

The thickener (stabilizer) may be present in an amount of 0.01 to 10 wt% based on the total weight of the composition, and specifically, 0.01 to 10 wt%, 0.01 to 5 wt%, 0.01 to 4 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1.5 wt%, 0.01 to 1 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.1 to 4 wt%, 0.1 to 3 wt%, 0.1 to 2 wt%, 0.1 to 1.5 wt%, 0.1 to 1 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 0.5 to 4 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, 0.5 to It may be present in an amount of 1.5 wt%, 0.5 to 1 wt%, 1 to 10 wt%, 1 to 5 wt%, 1 to 4 wt%, 1 to 3 wt%, 1 to 2 wt% or 1 to 1.5 wt%.

The above butter may include at least one selected from the group consisting of shea butter, mango seed butter, cacao seed butter, cupuacu seed butter, palm seed butter, triciriaimetica seed butter, kokum seed butter, borneo tallow seed butter and sala seed butter, but is not limited thereto.

The above higher alcohol may include at least one selected from the group consisting of cetyl alcohol, stearyl alcohol, behenyl alcohol, and batyl alcohol, but is not limited thereto.

The above-mentioned higher fatty acids may include at least one selected from the group consisting of stearic acid, isostearic acid, myristic acid and palmitic acid, but are not limited thereto.

The above cosmetic composition may further contain oil and/or silicone-based elastomer in addition to the ingredients described above.

The above oil is not limited to a type as long as it is an oil commonly used in cosmetics. For example, the above oil may be at least one selected from the group consisting of ester oil, hydrocarbon oil, silicone oil, vegetable oil, and triglyceride.

The above ester oil may be an ester compound of a fatty acid and a fatty alcohol. For example, the ester oil may include isopropyl myristate, isopropyl palmitate, ethylhexyl palmitate, cetyl octanoate, cetyl 2-ethyl hexanoate, 2-octyl dodecyl myristate, di-isostearyl malate, and the like.

The above hydrocarbon oil may include petroleum oils such as liquid paraffin, petrolatum, and isoparaffin; synthetic oils such as hydrogenated polydecene, synthetic squalane, and polybutene; and plant oils such as vegetable squalane and hydrogenated squalane.

The silicone oil may be an oil having a siloxane bond (Si-O-Si). For example, the silicone oil may include dimethicone, dimethiconol, phenyl trimethicone, disiloxane, cyclomethicone, cyclopentasiloxane, cyclohexasiloxane, and the like.

The above vegetable oil may be an oil obtained from a plant existing in nature. For example, the above vegetable oil may include olive oil, meadowfoam seed oil, rosehip fruit oil, avocado oil, camellia oil, apple seed oil, grape seed oil, sunflower seed oil, macadamia nut oil, coconut oil, castor oil, jojoba seed oil, caprylic/capric triglyceride, etc.

The above silicone elastomer may refer to a material in which a silicone cross-linked product is swelled in silicone oil and various oils. The silicone elastomer may include a silicone crosspolymer. The silicone crosspolymer may include, but is not limited to, a dimethicone crosspolymer, a vinyl dimethicone crosspolymer, and the like. The silicone elastomer may include a dimethicone/vinyl dimethicone crosspolymer.

The above cosmetic composition may further include purified water, a moisturizer, a pH regulator, a metal ion sequestering agent, and/or a preservative in addition to the ingredients described above.

The above preservative may include, but is not limited to, one or more selected from the group consisting of propanediol, ethylhexylglycerin, 1,2-hexanediol, and pentylene glycol.

The above moisturizer may include, but is not limited to, glycerin.

The above metal ion sequestering agent may include, but is not limited to, disodium EDTA.

In one embodiment, the cosmetic composition may be a water-in-oil type cosmetic composition comprising 1) an aqueous portion including a cell culture medium and albumin; and 2) an oily portion including at least one selected from the group consisting of a thickener (stabilizer), a surfactant, and a wax.

The aqueous portion of the above cosmetic composition may additionally include at least one selected from the group consisting of purified water, a metal ion sequestering agent, a moisturizer, and a preservative.

The oily portion of the above cosmetic composition may additionally include at least one selected from the group consisting of oil and silicone-based elastomer.

The above cosmetic composition may be manufactured in any formulation commonly manufactured in the art. For example, it may have any formulation selected from among skin products, lotions, essences, creams, packs, foundations, patches, hair tonics, micro needle patches, and makeup bases, but may not be limited thereto.

As an example of the above cosmetic composition formulation, skin care products including a softening toner, an astringent toner, a nourishing toner, a lotion, a gel, a cream, an essence, an eye essence, an eye cream, a nourishing cream, a massage cream, a clay pack, and a mask pack, makeup products including a lipstick, a lip tint, a lip gloss, a lip pencil, an eye shadow, a foundation, a powder, a concealer, an eyeliner, an eye shadow, and a mascara, eye removers, makeup removers, cleansing foams, cleansing creams, cleansing oils, cleansing water, hand sanitizers, hand wash, hand scrubs, body wash, body scrubs, shaving lotions, soaps, and cleaning products including wet tissues, body care products including a body lotion, a body oil, a body mist, a body essence, a hand cream, a foot cream, and a wax-type hair removal agent, infant cosmetics including a baby lotion, a baby cream, and a diaper rash cream, sunscreens, sun sticks, Sun care products including sun sprays, artificial tanning products, hair products including shampoos, rinses, hair conditioner creams, hair styling gels, foams, hair mousses, hair sprays, styling lotions, styling creams, hair essences, hair dyes, hair bleaching creams, curl activator gels, nail care products including nail polishes, cuticle removers, cuticle creams, toothpaste, mouthwashes, mouth rinses, mouth films, personal care products including gums, perfumes, plant extracts, deodorants, and antiperspirant formulations.

In one embodiment, the cosmetic composition may be an O/W (water-in-oil) emulsion formulation. The cosmetic composition of the present invention may be formulated as, for example, a liquid product, a lotion, an essence, a cream, a sun lotion, a sunscreen, a makeup base, a foundation, a BB cream, a stick product, or a balm-type product, but is not limited thereto. In addition, depending on the formulation to be manufactured, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a preservative, a fragrance, etc. may be additionally selected and mixed and added. In addition, a UV blocker, a light scattering agent, etc. may be included, and the formulation and added ingredients are not limited to the above contents. In addition, the ingredients may be introduced in an amount generally used in the field of dermatology.

The above cosmetic composition may contain additional ingredients commonly used in cosmetics, such as conventional adjuvants such as stabilizers, solvators, thickeners, dispersants, fragrances, fillers, preservatives, neutralizers, sweeteners, vitamins, antioxidants and metal ion sequestering agents. A person skilled in the art can select any additional ingredient and/or its amount such that the advantageous properties of the composition according to the present disclosure are not or are substantially not adversely affected by the expected addition.

Another aspect is to provide a water-in-oil type external skin composition comprising a cell culture medium and albumin. The same parts as described above are also applied to the composition.

The above skin external composition may be for improving skin condition.

The above skin condition improvement may include at least one selected from the group consisting of skin moisturizing, skin aging improvement, skin barrier strengthening, skin wrinkle improvement, skin elasticity enhancement, skin texture improvement, skin transparency enhancement, skin blemish removal, skin whitening, and skin regeneration.

The above skin external composition may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal delivery patch, a drug-containing bandage, a lotion, or a combination thereof. The skin external composition according to each formulation can be appropriately selected and mixed by a person skilled in the art according to the formulation or purpose of use of other skin external compositions, etc., in addition to the composition of the present invention, without difficulty, and in this case, a synergistic effect may occur when applied simultaneously with other raw materials.

The above skin external preparation may be appropriately mixed with ingredients commonly used in skin external preparations such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof, as needed.

The above skin external preparation may also appropriately contain metal sequestering agents such as sodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, licorice extract, glablidine, hot water extract of the fruit of Kalin, various crude drugs, tocopheryl acetate, glycyrrhizic acid, tranexamic acid and derivatives or salts thereof; and drugs such as vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, and trehalose.

The present invention relates to a water-in-oil type skin improvement cosmetic composition containing a cell culture medium and albumin. The cosmetic composition of the present invention provides stability of the formulation while containing a cell culture medium and albumin, and thus can be usefully used as a skin improvement cosmetic composition that can be smoothly spread and provides a feeling of nutrition without being sticky after absorption.

The present invention will be described in more detail through the following examples. However, these examples are provided for illustrative purposes only and the scope of the present invention is not limited to these examples.

Comparative Example 1: Preparation of a composition containing cell culture medium and albumin

The components of the cell culture medium and albumin described in Table 1 below were sufficiently dissolved in a solvent (e.g., purified water, etc.) to confirm that all components were dissolved, and then filtered through a 0.2 ㎛ filter to prepare a mixed composition containing the following 23 components. Each component described in Table 1 below was purchased from Sigma-Aldrich (Sigma-Aldrich, USA), and the combination of the 23 components described in Table 1 below is referred to as SPM hereinafter.

Meanwhile, the concentrations listed in Table 1 refer to the final concentrations in the solution when the above components are completely dissolved in the solvent.

ingredient Concentration (g/L) Glycine 0.01875 L-alanine 0.00445 L-arginine hydrochloride 0.1475 L-cysteine hydrochloride-monohydrate-H ₂ O 0.01756 L-glutamine 0.365 L-histidine hydrochloride-monohydrate 0.03148 L-lysine hydrochloride 0.09125 L-methionine 0.01724 L-proline 0.01725 L-serine 0.02625 L-threonine 0.05345 L-valine 0.05285 i-inositol 0.0126 Thiamine hydrochloride
(thiamine hydrochloride) 0.00217 Niacinamide
(Niacinamide) 0.00202 Pyridoxine hydrochloride
(pyridoxine hydrochloride) 0.002 Sodium chloride (NaCl) 3.49775 Sodium bicarbonate (NaHCO ₃ ) 2.438 Potassium chloride (KCl) 0.3118 Calcium chloride (CaCl ₂ )(anhydrous) 0.1166 Sodium phosphate monobasic monohydrate (NaH ₂ PO ₄ -H ₂ O) 0.0625 sodium pyruvate 0.055 albumin 0.1

Manufacturing Example 2: Manufacturing of a water-in-oil type cosmetic composition containing cell culture medium and albumin

To prepare a water-in-oil type cosmetic composition containing a cell culture medium and albumin, the following experiments were performed.

Specifically, the oil phase was added to a beaker according to the content ratios shown in Tables 2 to 4 below, and then stirred at 2,500 rpm at 75°C for 5 minutes using a homomixer to confirm complete dissolution. Next, the dissolved water phase was slowly added to the oil phase while stirring at 6,500 rpm for 5 minutes, and then cooled to 30°C to prepare a cosmetic composition.

Meanwhile, Table 2 below shows cosmetic compositions prepared by changing the surfactant, Table 3 below shows cosmetic compositions prepared by changing the thickener (stabilizer), and Table 4 below shows cosmetic compositions prepared by changing the wax.

Raw material name Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Paid part Cyclopentasiloxane 7.00 7.00 7.00 7.00 7.00 Phenyl trimethicone 5.00 5.00 5.00 5.00 5.00 PEG-10 Dimethicone - 3.00 - - - PEG/PPG-18/18 Dimethicone - - 3.00 - - Cetyl PEG/PPG-10/1 Dimethicone - - - 3.00 - Bis-PEG/PPG-14/14 Dimethicone - - - - 3.00 Hectorite 1.00 1.00 1.00 1.00 1.00 Beeswax 1.00 1.00 1.00 1.00 1.00 Dimethicone/Vinyl Dimethicone Crosspolymer 0.50 0.50 0.50 0.50 0.50 Awards Purified water to 100 to 100 to 100 to 100 to 100 Cell culture medium and albumin (SPM) 0.78 0.78 0.78 0.78 0.78 Disodium EDTA 0.02 0.02 0.02 0.02 0.02 glycerin 5.00 5.00 5.00 5.00 5.00 Propanediol 5.00 5.00 5.00 5.00 5.00 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 1,2-Hexanediol 1.00 1.00 1.00 1.00 1.00 Pentylene glycol 1.50 1.50 1.50 1.50 1.50

Raw material name Comparative Example 2 Example 5 Example 6 Example 7 Example 8 Paid part Cyclopentasiloxane 7.00 7.00 7.00 7.00 7.00 Phenyl trimethicone 5.00 5.00 5.00 5.00 5.00 PEG-10 Dimethicone 3.00 3.00 3.00 3.00 3.00 Hectorite - 1.00 - - - Quaternium-18 hectorite - - 1.00 - - Stearalkonium hectorite - - - 1.00 - Disteardimonium hectorite - - - - 1.00 Beeswax 1.00 1.00 1.00 1.00 1.00 Dimethicone/Vinyl Dimethicone Crosspolymer 0.50 0.50 0.50 0.50 0.50 Awards Purified water to 100 to 100 to 100 to 100 to 100 Cell culture medium and albumin 0.78 0.78 0.78 0.78 0.78 Disodium EDTA 0.02 0.02 0.02 0.02 0.02 glycerin 5.00 5.00 5.00 5.00 5.00 Propanediol 5.00 5.00 5.00 5.00 5.00 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 1.2 Hexanediol 1.00 1.00 1.00 1.00 1.00 Pentylene glycol 1.50 1.50 1.50 1.50 1.50

Raw material name Comparative Example 3 Example 9 Example 10 Example 11 Example 12 Paid part Cyclopentasiloxane 7.00 7.00 7.00 7.00 7.00 Phenyl trimethicone 5.00 5.00 5.00 5.00 5.00 PEG-10 Dimethicone 3.00 3.00 3.00 3.00 3.00 Hectorite 1.00 1.00 1.00 1.00 1.00 Beeswax - 1.00 - - - Candelilla wax - - 1.00 - - Carnauba wax - - - 1.00 - Ceresin wax - - - - 1.00 Dimethicone/Vinyl Dimethicone Crosspolymer 0.50 0.50 0.50 0.50 0.50 Awards Purified water to 100 to 100 to 100 to 100 to 100 Cell culture medium and albumin 0.78 0.78 0.78 0.78 0.78 Disodium EDTA 0.02 0.02 0.02 0.02 0.02 glycerin 5.00 5.00 5.00 5.00 5.00 Propanediol 5.00 5.00 5.00 5.00 5.00 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 1.2 Hexanediol 1.00 1.00 1.00 1.00 1.00 Pentylene glycol 1.50 1.50 1.50 1.50 1.50

Experimental Example 1: Stability Test of Cosmetic Formulation According to Temperature Change

In order to confirm the stability of the water-in-oil type cosmetic formulation of the present invention according to temperature change, the following experiment was conducted.

Specifically, the long-term storage stability of the cosmetic formulations of Comparative Examples 1 to 3 and Examples 1 to 12 was evaluated based on the evaluation criteria below in a constant temperature bath of 4°C, 45°C, and 50°C, in a cycle (-15°C to 45°C), and at room temperature for 1 week, 1 month, and 6 months, and the results are shown in Tables 5 to 7 below (evaluation criteria, stable: ○, unstable: △, separated: ×).

temperature period Comparative Example 1 Example 1 Example 2 Example 3 Example 4 4℃ 1 week X ○ ○ ○ ○ 1 month X ○ ○ ○ ○ 6 months X ○ ○ ○ ○ Room temperature 1 week X ○ ○ ○ ○ 1 month X ○ ○ ○ ○ 6 months X ○ ○ ○ ○ 45 ℃ 1 week X ○ ○ ○ ○ 1 month X ○ ○ ○ ○ 6 months X ○ ○ ○ ○ 50℃ 1 week X ○ ○ ○ ○ 1 month X ○ ○ ○ ○ 6 months - - - - - Cycle
(-15~45℃) 1 week X ○ ○ ○ ○ 1 month X △ △ △ △ 6 months - - - - -

temperature period Comparative Example 2 Example 5 Example 6 Example 7 Example 8 4℃ 1 week ○ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ Room temperature 1 week ○ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ 45 ℃ 1 week ○ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ 50℃ 1 week △ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months - - - - - Cycle
(-15~45℃) 1 week △ ○ ○ ○ ○ 1 month X △ △ △ △ 6 months - - - - -

temperature period Comparative Example 3 Example 9 Example 10 Example 11 Example 12 4 ℃ 1 week ○ ○ ○ ○ ○ 1 month ○ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ Room temperature 1 week ○ ○ ○ ○ ○ 1 month ○ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ 45 ℃ 1 week ○ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months △ ○ ○ ○ ○ 50℃ 1 week △ ○ ○ ○ ○ 1 month △ ○ ○ ○ ○ 6 months - - - - - Cycle
(-15~45℃) 1 week △ ○ ○ ○ ○ 1 month X △ △ △ △ 6 months - - - - -

As shown in Tables 5 to 7 above, the formulations of Comparative Examples 1 to 3 that did not use a surfactant, thickener (stabilizer), or wax were unstable, but those of Examples 1 to 12 were found to have excellent stability. Based on the above results, it can be seen that there is no problem with the stability of the water-in-oil formulation containing the cell culture medium and albumin of the present invention due to the effective ingredient of the formulation.

The above description of the present invention is for illustrative purposes only, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential characteristics of the present invention. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive.

Claims (18)

A water-in-oil (W/O) cosmetic composition comprising a cell culture medium and albumin. In claim 1, the cell culture medium contains amino acid components, vitamin components, mineral salt components, and other components.
The amino acid component comprises, based on 1 part by weight of the amino acid serine, 0.2 to 1.2 parts by weight of glycine, 0.08 to 0.3 parts by weight of L-alanine, 2 to 7 parts by weight of L-arginine hydrochloride, 0.2 to 0.9 parts by weight of L-cysteine hydrochloride-monohydrate, 10 to 18 parts by weight of L-glutamine, 0.8 to 2.0 parts by weight of L-histidine hydrochloride-monohydrate, 1.0 to 5.0 parts by weight of L-lysine hydrochloride, 0.2 to 1.2 parts by weight of L-methionine, 0.2 to 1.2 parts by weight of L-proline, 1 part by weight of L-serine, 1.0 to 5.0 parts by weight of L-threonine, and 1.0 to 5.0 parts by weight of L-valine,
The above vitamin ingredients include i-inositol, thiamine hydrochloride, niacinamide and pyridoxine hydrochloride.
The above inorganic salt components include sodium chloride (NaCl), sodium bicarbonate (NaHCO ₃ ), potassium chloride (KCl), calcium chloride (CaCl ₂ ) (anhydrous), and sodium biphosphate monohydrate (NaH ₂ PO ₄ -H ₂ O).
A composition wherein the other ingredients include sodium pyruvate. A composition according to claim 2, wherein the vitamin component comprises 0.2 to 0.9 parts by weight of i-inositol, 0.04 to 0.8 parts by weight of thiamine hydrochloride, 0.04 to 0.8 parts by weight of niacinamide, and 0.04 to 0.8 parts by weight of pyridoxine hydrochloride based on 1 part by weight of amino acid serine. A composition according to claim 2, wherein the inorganic salt component comprises 50 to 200 parts by weight of sodium chloride (NaCl), 50 to 120 parts by weight of sodium bicarbonate (NaHCO ₃ ), 2.0 to 20 parts by weight of potassium chloride (KCl), 1.0 to 10 parts by weight of calcium chloride (CaCl ₂ ) (anhydrous), and 0.5 to 5.0 parts by weight of sodium biphosphate monohydrate (NaH ₂ PO _4- H ₂ O) based on 1 part by weight of the amino acid serine. A composition according to claim 2, wherein the other component comprises 0.5 to 5.0 parts by weight of sodium pyruvate based on 1 part by weight of amino acid serine. A composition according to claim 1, wherein the albumin is human serum albumin or bovine serum albumin. A composition according to claim 1, wherein the albumin is 10 to 1000 μg/㎖. A cosmetic composition according to claim 1, characterized in that the cell culture medium and albumin are in an amount of 0.0001 to 10 wt% based on the total weight of the composition. A composition according to claim 1, wherein the composition is for improving skin condition. A composition according to claim 9, wherein the improvement in skin condition includes at least one selected from the group consisting of skin moisturizing, skin aging improvement, skin barrier strengthening, skin wrinkle improvement, skin elasticity enhancement, skin texture improvement, skin transparency enhancement, skin blemish removal, skin whitening, and skin regeneration. A composition according to claim 1, wherein the composition further comprises at least one selected from the group consisting of a surfactant, a wax, a butter, a thickener, a higher alcohol, and a higher fatty acid. In claim 11, the surfactant is selected from the group consisting of PEG-8 dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone, PEG-10 dimethicone, PEG-12 dimethicone, PEG-11 methyl ether dimethicone, cetyl PEG/PPG dimethicone, cetyl PEG/PPG-10/1 dimethicone, bis-PEG/PPG-14/14 dimethicone, bis-PEG/PPG-20/20 dimethicone, PEG/PPG-18/18 dimethicone, PEG/PPG-19/19 dimethicone, PEG/PPG-20/20 dimethicone, polyglyceryl-3 polydimethylsiloxyethyl dimethicone, lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone, and lauryl PEG-9. A composition comprising at least one selected from the group consisting of polydimethylsiloxyethyl dimethicone. A composition according to claim 11, wherein the wax comprises at least one selected from the group consisting of beeswax, candelilla wax, carnauba wax, multiwax, paraffin wax, microcrystal wax, and ceresin wax. A composition according to claim 11, wherein the thickener comprises at least one selected from the group consisting of hectorite, quaternium-18 hectorite, stearalkonium hectorite, and disteardimonium hectorite. A composition according to claim 11, wherein the butter comprises at least one selected from the group consisting of shea butter, mango seed butter, cacao seed butter, cupuacu seed butter, palm seed butter, triciriaimetica seed butter, kokum seed butter, borneo tallow seed butter, and salicornia seed butter. A composition according to claim 11, wherein the higher alcohol comprises at least one selected from the group consisting of cetyl alcohol, stearyl alcohol, behenyl alcohol, and batyl alcohol. A composition according to claim 11, wherein the higher fatty acid comprises at least one selected from the group consisting of stearic acid, isostearic acid, myristic acid, and palmitic acid. A water-in-oil type external skin preparation composition comprising a cell culture medium and albumin.