KR20240133267A - Human Biological Material extracting device for Liquid Biopsy and extracting method - Google Patents

Abstract

The present invention relates to an automated device for extracting human-derived materials from patient samples in a single device, extracting ctDNA from plasma in the patient's blood and CTC from PBMC in a single device, thereby reducing the burden on the patient, increasing reproducibility compared to conventional manual work, and simplifying extraction in an automated manner, and to a human-derived material extraction method using the same for liquid biopsy.
Accordingly, the present invention comprises: a container storage member (100) for storing a working container (10), a reagent container (20), and a disposable tip (30); a dispensing module (220) equipped with a plurality of syringes (221) for aspirating and dispensing a target material, and a fluid transport member (200) for transporting the dispensing module (220) in the X, Y, and Z directions; a container transport member (300) having a gripper (320) for transporting the working container (10) and transporting the gripper (320) in the X, Y, and Z directions; and an extraction member (400) for mixing a sample and a reagent within the settled working container (10) and extracting a human-derived material from the mixed sample and reagent when the working container (10) into which a reagent has been dispensed onto a sample through the fluid transport member (200) is settled through the container transport member (300).

Description

{Human Biological Material extracting device for Liquid Biopsy and extracting method using the same}

The present invention relates to an automated device for extracting human-derived materials from patient samples in a single device, extracting ctDNA from plasma in the patient's blood and CTC from PBMC in a single device, thereby reducing the burden on the patient, increasing reproducibility compared to conventional manual work, and simplifying extraction in an automated manner, and to a human-derived material extraction method using the same for liquid biopsy.

In general, for devices that extract ctDNA and CTC materials from patient samples, DNA extraction and CTC separation from patient samples are performed manually, or extraction or separation is performed separately in equipment with each function.

Meanwhile, cfDNA is cell free DNA derived from cells, and ctDNA is Circulating Tumor DNA derived from tumor cells and falls within the scope of cfDNA.

Extraction of these ctDNA and CTC materials involves extracting ctDNA from plasma and CTC from PBMC in the blood of the same patient, and the extracted ctDNA can be used for early cancer diagnosis by analyzing the concentration and degree of CpG methylation.

However, in the past, ctDNA was extracted manually, but the concentration was not constant depending on the extraction environment (personnel, reagents, equipment, etc.), so there was a problem of low reproducibility, which limited molecular genetic analysis (PCR or NGS, etc.).

Korean Patent Publication No. 10-2441735 (registered on September 5, 2022)

The present invention is intended to solve the problems of the related art, and the purpose of the present invention is to provide an automated device that enables extraction of ctDNA and CTC materials in a fully automated manner by using a container storage member having a rack in which a working container, a reagent container, a disposable tip, etc. are stored, a fluid transfer member that transfers fluid using a plurality of syringes, a container transfer member having a gripper for transferring the working container, and an extraction member for mixing a sample and a reagent in the working container and extracting a human-derived material, and an extraction method using the same.

In addition, the present invention aims to provide an automated device that enables molecular genetic analysis (such as PCR or NGS) while overcoming the limitation of lower reproducibility compared to existing manual work according to an automated method, so that the developed device can be used as an in vitro diagnostic medical device, allowing users (such as clinical pathologists) to easily extract samples from patients in the field (such as hospitals and examination centers).

To solve this purpose, the present invention;

A container storage member for storing work containers, reagent containers, and disposable tips;

A dispensing module equipped with a plurality of syringes for suction and dispensing a target material is provided, and a fluid transport member for transporting the dispensing module in the X, Y, and Z directions;

A container transfer member having a gripper for transferring a work container and allowing the gripper to be transferred in the X, Y, and Z directions;

The present invention provides an automated device for extracting human-derived materials, characterized in that it comprises an extraction member that mixes the sample and the reagent within the settled working vessel when the working vessel into which the reagent is dispensed is settled through the fluid transfer member, and extracts human-derived materials from the mixed sample and reagent.

In addition, the present invention;

(S1) A step of using a dispensing module of a fluid transport member to suck up a reagent from a corresponding reagent container stored in a container storage member and then dispensing it to a working container corresponding to the order of the first rack section;

(S2) A step of moving the working vessel into which the reagent is dispensed to the first working table of the extraction vessel using a vessel transfer member;

(S3) A step of lowering the extraction tip into the work container of the first work table and then mixing the sample and reagent by moving it up and down for a set time;

(S4) A step of moving a work container corresponding to the order of the first rack section of the container storage member to the second work table of the extraction member using a container transfer member;

(S5) A step of lowering the extraction tip to the bottom of the working container of the first working table;

(S6) A step of lowering the magnet bar so that it is inserted into the extraction tip and then applying a magnetic field to attach the human-derived material to the outside of the extraction tip;

(S7) A step of simultaneously raising the extraction tip and the magnet bar to extract human-derived material from the working container of the first working table;

(S8) A step of moving the work container of the second work table to the location of the extraction tip and the magnet bar from which the human-derived material is extracted;

(S9) A step of lowering the extraction tip and magnet bar to the bottom of the working container of the second working table;

(S10) A step of raising the magnet bar so that it is separated from the extraction tip;

(S11) A step of moving the extraction tip up and down to cause the human-derived material attached to the extraction tip to fall into the working container;

(S12) A step of completing extraction by lifting the extraction tip away from the working container;

(S13) A method for extracting human-derived substances is provided, characterized by comprising a step of sequentially moving the work containers of the first worktable and the work containers of the second worktable in the order corresponding to the first rack section of the container storage member.

According to the present invention, by providing an automated ctDNA and CTC extraction device, extraction of ctDNA and CTC materials is easy, places less burden on the patient, and overcomes the limitation of low reproducibility compared to existing manual work, thereby enabling molecular genetic analysis (PCR or NGS, etc.), so that the developed device can be used as an in vitro diagnostic medical device, allowing the user to easily extract ctDNA and CTC materials using a patient's specimen in the field.

Figure 1 is a diagram showing the overall configuration of an automated device for extracting human-derived materials according to one embodiment of the present invention.
Figure 2 is a configuration diagram of a container storage member in the automated human-derived material extraction device of Figure 1.
Figure 3 is a configuration diagram of the working container and reagent container in the automated human-derived material extraction device of Figure 1.
Figure 4 is a configuration diagram of a disposable tip in the automated device for extracting human-derived materials of Figure 1.
Figure 5 is a configuration diagram of the cooling unit of the automated human-derived material extraction device of Figure 1.
Figure 6 is a configuration diagram of the heating unit of the automated device for extracting human-derived materials of Figure 1.
Figure 7 is a configuration diagram of the fluid transfer member and container transfer member of the automated human-derived material extraction device of Figure 1.
Figure 8 is a configuration diagram of an extraction member in the automated human-derived material extraction device of Figure 1.
Fig. 9 is a configuration diagram of the extraction tip and magnet bar in the absence of the extraction device of Fig. 8.
Figure 10 is a flow chart of a method for extracting human-derived materials according to one embodiment of the present invention.

The features of the automated human-derived material extraction device according to the present invention and the human-derived material extraction method using the same will be understood through the following detailed description of embodiments with reference to the drawings.

Meanwhile, embodiments of the present invention may be implemented in various embodiments within the scope of the technical field to which the invention belongs, and are not specifically limited to the embodiments described herein.

In addition, in order to clearly explain the present invention with respect to the drawings, parts irrelevant to the description are omitted from the drawings, and the same or similar drawing symbols are used for the same or similar components throughout the specification, and when describing common components, the drawing symbols for these are omitted.

Hereinafter, the present invention will be described with reference to the attached drawings.

FIGS. 1 to 9 are drawings for explaining an automated device for extracting human-derived materials according to a basic embodiment of the present invention.

The automated device (1) for extracting human-derived substances according to the present invention comprises: a container storage member (100) for storing a working container (10), a reagent container (20), and a disposable tip (30); a fluid transport member (200) provided with a dispensing module (220) equipped with a plurality of syringes (221) for aspirating and dispensing a target substance, and transporting the dispensing module (220) in the X, Y, and Z directions; a container transport member (300) having a gripper (320) for transporting the working container (10), and transporting the gripper (320) in the X, Y, and Z directions; and an extraction member (400) for mixing the sample and the reagent within the settled working container (10) and extracting the human-derived substance from the mixed sample and reagent when the working container (10) into which the reagent is dispensed onto the sample through the fluid transport member (200) is settled through the container transport member (300).

First, the container storage member (100) is provided on the lower inner side of the main body of the automatic human-derived material extraction device (1), as illustrated in FIG. 1, and a plurality of working containers (10), a plurality of reagent containers (20), and a plurality of disposable tips (30) are stored on the upper side of the container storage member (100).

At this time, some of the plurality of work containers (10) accommodate patient samples, a plurality of reagent containers (20) are equipped with reagents (including magnetic beads) used to extract ctDNA and CTC materials, which are human-derived materials, and a plurality of disposable tips (30) are installed in a replaceable manner in a syringe (221) equipped in a dispensing module (220) of a fluid transfer member (200).

Meanwhile, the container storage member (100) further includes a first rack section (110) in which a plurality of working containers (10) are stored in sequence as shown in FIG. 2; a second rack section (120) in which a plurality of disposable tips (30) according to capacity are stored; and a third rack section (130) in which a plurality of reagent containers (20) are stored for storage at room temperature.

The first rack section (110) is configured to store a total of six work containers (10), and as shown in the configuration of Fig. 2, the first rack section (110) has racks 1, 2, and 3 from the inner center, and racks 4, 5, and 6 from the outer center, and the work containers (10) are stored by being stored on the tops of these racks.

At this time, the work container (10) is formed in the form of a tray with receiving spaces arranged at regular intervals in the horizontal and vertical directions as shown in Fig. 3.

The second rack section (120) is equipped with two racks on the outside as shown in Fig. 2 so that a number of disposable tips (30) can be stored according to capacity, and disposable tips (30) of different capacities are placed and stored on trays at regular intervals in the horizontal and vertical directions on the top of these racks.

At this time, the disposable tip (30) may be provided in different shapes according to capacity as shown in Fig. 4, but is not particularly limited thereto.

The third rack section (130) is provided with one rack between the first rack section (110) and the second rack section (120), and reagent containers (20) filled with reagents that can be stored at room temperature are placed and stored on trays at regular intervals in the horizontal and vertical directions on the top of the rack of the third rack section (130).

In addition, the container storage member (100) further includes a cooling unit (140) that performs cooling of the working container (10) and the reagent container (20).

The cooling unit (140) further includes a first cooling module (141) in which a plurality of reagent containers (20) according to capacity are respectively installed, and which performs cooling of the reagent containers (20), as shown in FIG. 5; and a second cooling module (142) in which a work container (10) is installed, and which performs cooling of the work container (10).

The first cooling module (141) is installed adjacent to the third rack section (130) between the first rack section (110) and the second rack section (120), and as shown in the configuration of FIG. 5, a first cooling plate (141a) is provided on the upper portion, on which a plurality of reagent containers (20) according to capacity are respectively installed, and a thermoelectric element (41), a temperature sensor (42), a heat sink (43), and a heat sink fan (44) are installed below the first cooling plate (141a) to perform cooling of the reagent containers (20) installed on the first cooling plate (141a).

At this time, the reagent container (20) may be provided in different shapes according to capacity as shown in Fig. 3, but is not particularly limited thereto.

In this case, depending on the human-derived target material, the reagent contained in the reagent container (20) in the third rack section (130) that is stored at room temperature can be used, or the reagent contained in the reagent container (20) in the first cooling module (141) can be selectively used, and the reagent includes magnetic beads.

The second cooling module (142) is installed on the side adjacent to rack 1 of the first rack section (110), and as shown in the configuration of FIG. 5, a second cooling plate (142a) on which a work container (10) is installed is provided at the upper portion, and a thermoelectric element (41), a temperature sensor (42), a heat sink (43), and a heat sink fan (44) are installed at the lower portion of the second cooling plate (142a) to perform cooling of the work container (10) installed on the second cooling plate (142a).

In addition, the container storage member (100) further includes a heating member (150) for heating the working container (10).

The heating unit (150) is provided on the adjacent side of the second cooling module (142), and as in the configuration of FIG. 6, a heating plate (151) is provided on which a working container (10) is mounted, and a cartridge heater (51) and a temperature sensor (52) are provided at the bottom of the heating plate (151) to perform cooling of the working container (10) mounted on the heating plate (151).

And, as illustrated in FIG. 1, the fluid transfer member (200) is provided with a dispensing module (220) equipped with a plurality of syringes (221) on the upper side of the container storage member (100) inside the main body of the human-derived material extraction automation device (1) so as to be movable in the X, Y, and Z directions, and the dispensing module (220) is used to suck up a reagent (and magnetic beads included) from a reagent container (20) containing the corresponding reagent for human-derived material extraction, and then dispense the reagent into the corresponding working container (10) for human-derived material extraction among the plurality of working containers (10) mounted on the first rack section (110) of the container storage member (100).

Meanwhile, the fluid transport member (200) further includes a first transport unit (210) that transports the dispensing module (220) in the X, Y, and Z directions as illustrated in FIG. 7; and a dispensing module (220) equipped with a plurality of syringes (221) each having a disposable tip (30) for each capacity at the bottom, which are transported in the X, Y, and Z directions through the first transport unit (210).

The first transfer unit (210) is equipped with a linear actuator that can move in the X-axis direction, and a Y-axis linear actuator is connected to the lower portion of the X-axis linear actuator. A dispensing module (220) is connected to the lower portion of the Y-axis linear actuator to enable movement of the dispensing module (220) in the X and Y-axis directions.

At this time, the dispensing module (220) is arranged vertically in a row so that a plurality of syringes (221) can be equipped with disposable tips (30) according to capacity on both sides as in the configuration of Fig. 7, and a Z-axis linear actuator is provided on the side where the plurality of syringes (221) are arranged so that the plurality of syringes (221) can move in the Z-axis direction.

In addition, the fluid transfer member (200) is provided at the bottom of a plurality of syringes (221) as shown in FIG. 7, and is capable of horizontally reciprocating toward the side where disposable tips (30) for each capacity are provided, and further includes a drop catcher (230) that receives a substance flowing out from the disposable tips (30).

The drop catcher (230) is provided with an X-axis linear actuator at the bottom of the dispensing module (220), and a drop catcher (230) corresponding to the spacing between a plurality of syringes (221) provided on both sides of the dispensing module (220) in a roughly ‘ㄷ’ shape is connected to the X-axis linear actuator, so that the drop catcher (230) moves back and forth between the plurality of syringes (221) on both sides.

At this time, the drop catcher (230) moves to the bottom of the syringe (221) on the side where the reagent is sucked and receives the sucked reagent falling downward through the disposable tip (30) to prevent leakage.

And, as illustrated in FIG. 1, the container transfer member (300) is provided so that the gripper (320) can move in the X, Y, and Z directions on the side adjacent to the fluid transfer member (200) inside the main body of the human-derived material extraction automation device (1), and the work container (10) is moved to the target position during the human-derived material extraction process using the gripper (320).

Meanwhile, the container transport member (300) further includes a second transport member (310) that transports the gripper (320) in the X, Y, and Z directions as illustrated in FIG. 7; and a gripper (320) that is transported in the X, Y, and Z directions through the second transport member (310) and is capable of selectively gripping the work container (10).

The second transport unit (310) is equipped with a linear actuator that can move in the X-axis direction, and a Y-axis linear actuator is connected to the X-axis linear actuator. A gripper (320) is connected to the lower portion of the Y-axis linear actuator to enable movement of the gripper (320) in the X and Y-axis directions.

The gripper (320) is provided at the bottom of a linear actuator that can move in the Z-axis direction and can move up and down, and when the gripper (320) is provided on a bidirectional actuator, it can hold the corresponding work container (10) with a selective gripping action and move it to a target position.

And the extraction member (400) is provided at the lower part of the fluid transfer member (200) and the container transfer member (300) inside the main body of the human-derived material extraction automation device (1), as illustrated in FIG. 1, and the working container (10) stored in the container storage member (100) is transferred and settled through the container transfer member (300), and the sample and reagent (including magnetic beads) are mixed within the settled working container (10), and the human-derived material is extracted from the mixed sample and reagent.

Meanwhile, the extraction member (400) further includes first and second work platforms (410, 420) on which work containers (10) for different purposes are respectively installed, as shown in FIG. 8; and a third transport unit (430) that moves the first and second work platforms (410, 420) to a target location.

The first and second work platforms (410, 420) are each provided to be movable in the Y-axis direction on top of the third transfer unit (430), and the first work platform (410) and the second work platform (420) operate independently by the third transfer unit (430), and the work container (10) is installed on the top of the first and second work platforms (410, 420).

The third transfer unit (430) is configured in such a way that linear actuators that operate independently in the Y-axis direction are respectively provided at the positions of the first worktable (410) and the second worktable (420), and the third transfer unit (430) moves the first worktable (410) and the second worktable (420) to the appropriate positions during the process of extracting human-derived materials.

In this case, the sample and reagent (including magnetic beads) are mixed in the work container (10) of the first work table (410), the human-derived material is extracted from the mixed sample and reagent, and the extracted human-derived material is transferred to the work container (10) of the second work table (420), thereby extracting the human-derived material.

In addition, the extraction member (400) further includes an extraction tip (440) that mixes a sample and a reagent by operating up and down for a set time while being inserted into the interior of a working container (10) as shown in FIGS. 8 and 9; a magnet bar (450) that is vertically positioned on the upper portion of the extraction tip (440), can be inserted into the interior of the extraction tip (440) through an up and down operation, and extracts a human-derived substance according to the application of a magnetic field;

The extraction tip (440) is connected to a linear actuator in the Z-axis direction and operates up and down. As shown in the configuration of FIG. 9, a plurality of extraction tips (440) are vertically arranged at regular intervals in the horizontal and vertical directions. The extraction tip (440) is formed in the form of a container with an open top and has a head (441) that protrudes in the shape of a plate so that the sample and reagent (including magnetic beads) can be easily mixed according to the up and down operation of the lower portion of the extraction tip (440).

The magnet bar (450) is connected to a linear actuator in the Z-axis direction at the top of the extraction tip (440) and operates up and down. As shown in the configuration of FIG. 9, a plurality of magnet bars (450) are vertically arranged at a certain interval in the horizontal and vertical directions corresponding to the arrangement of a plurality of extraction tips (440), and depending on the up and down operation, the magnet bar (450) can be inserted into the inside of the extraction tip (440) through the upper opening of the extraction tip (440).

In this case, the extraction tip (440) moves up and down within the working vessel (10) for a set period of time to mix the sample and the reagent (including magnetic beads), and the magnet bar (450) is inserted into the interior of the extraction tip (440) after the mixing in the working vessel (10) is completed, and then a magnetic field is applied to attach human-derived material from the mixture of the sample and reagent within the working vessel (10) to the outside of the extraction tip (440) to perform extraction.

At this time, the process of attaching human-derived materials is briefly explained. As the sample and reagent are mixed, human-derived materials are attached to the surface of the magnetic beads, and the magnetic beads with human-derived materials attached are attached to the outside of the extraction tip (440) by magnetization of the magnet bar (450), and the human-derived materials can be obtained by extracting the magnetic beads.

Meanwhile, the automated device (1) for extracting human-derived substances of the aforementioned structure controls the operation of the container storage member (100), the fluid transport member (200), the container transport member (300), and the extraction member (400) through a control module. To this end, the control module may be equipped with a monitor, input device, etc. that allows the user to input and modify a protocol.

At this time, the control module can utilize position values provided through various sensors, temperature measurement values provided through temperature sensors of the cooling unit (140) and the heating unit (150), etc., and enables cooling and heating for a preset period of time, and automates the overall process of extracting human-derived substances through operation control of the fluid transport member (200), container transport member (300), and extraction member (400).

Hereinafter, a method for extracting human-derived materials according to an embodiment of the present invention will be described with reference to FIG. 10.

According to this, the method for extracting human-derived materials using the aforementioned automated human-derived material extraction device (1) is as follows:

(S1) A step of sucking the reagent (including magnetic beads) from the corresponding reagent container (20) stored in the container storage member (100) using the dispensing module (220) of the fluid transfer member (200) and dispensing it to the working container (10) corresponding to the order of the first rack section (110);

(S2) A step of moving the working container (10) in which the reagent is dispensed to the first working table (410) of the extraction member (400) using the container transfer member (300);

(S3) A step of lowering the extraction tip (440) into the work container (10) of the first work table (410) and then mixing the sample and reagent by moving it up and down for a set time;

(S4) A step of moving a work container (10) corresponding to the order of the first rack section (110) of the container storage member (100) to the second work table (420) of the extraction member (400) using the container transfer member (300);

(S5) A step of lowering the extraction tip (440) to the bottom of the work container (10) of the first work table (410);

(S6) A step of lowering the magnet bar (450) so that it is inserted into the extraction tip (440) and then applying a magnetic field to attach the human-derived material to the outside of the extraction tip (440);

(S7) A step of simultaneously raising the extraction tip (440) and the magnet bar (450) to extract human-derived material from the work container (10) of the first work table (410);

(S8) A step of moving the work container (10) of the second work table (420) to the position of the extraction tip (440) and the magnet bar (450) from which the human-derived material is extracted;

(S9) A step of lowering the extraction tip (440) and the magnet bar (450) to the bottom of the working container (10) of the second working table (420);

(S10) A step of raising the magnet bar (450) so as to separate it from the extraction tip (440);

(S11) A step of moving the extraction tip (440) up and down to cause the human-derived material attached to the extraction tip (440) to fall into the working container (10);

(S12) A step of completing extraction by lifting the extraction tip (440) away from the working container (10);

(S13) It comprises a step of sequentially moving the work container (10) of the first work platform (410) and the work container (10) of the second work platform (420) in the order corresponding to the first rack section (110) of the container storage member (100).

In addition, the above step (S3)

(S3-1) Optionally, a step is further included of cooling the working container (10) in which the mixing of the sample and the reagent is completed using the cooling unit (140) of the container storage unit (100) or heating it using the heating unit (150) using the container transfer unit (300).

In addition, the method for extracting human-derived materials further includes optionally repeating steps (S1) to (S13) an arbitrary number of times according to the order of the working containers (10) stored in the first rack section (110) of the container storage member (100).

For example, in the case of extracting human-derived material from a cfDNA target, the protein removal process (process of removing proteins present in plasma) is performed through the aforementioned steps (S1) to (S13) and (S3-1), and after this process is completed, the aforementioned steps (S1) to (S13) are sequentially repeated for each of the Washing 1, Washing 2, Washing 3, and Elution processes.

In this case, the Protein removal process is performed by extracting a human-derived material from the first working container (10) in the order of the first rack section (110) of the container storage member (100) and then transferring the human-derived material to the second working container (10), and Washing 1 is performed by extracting a human-derived material from the second working container (10) and then transferring the human-derived material to the third working container (10), and the remaining Washing 2, Washing 3, and Elution processes are performed sequentially according to the order of the working containers (10) of the first rack section (110) of the container storage member (100).

In addition, when extracting human-derived material for the CTC target, the steps (S1) to (S13) described above are performed, and the steps (S1) to (S13) described above can be repeated a specific number of times according to the order of the working container (10) of the first rack section (110).

At this time, when steps (S1) to (S13) described above are repeatedly performed while extracting human-derived material of the CTC target, the reagent dispensing step of step (S1) may be omitted.

Meanwhile, depending on the target to be extracted, such as ctDNA or CTC material, the reagent contained in the reagent container (20) in the third rack section (130) that is stored at room temperature can be used, or the reagent contained in the reagent container (20) in the first cooling module (141) can be selectively used.

In this case, as a preferred embodiment using the steps of the method for extracting human-derived materials described above, examples of extracting ctDNA or CTC materials have been described, but the steps of the method for extracting human-derived materials of the present invention are not particularly limited to these examples, and the steps described above can be utilized in various ways depending on the human-derived target material, extraction environment, etc.

As described above, the present invention has been described with reference to specific preferred embodiments and drawings, but the terms used herein are intended to facilitate the description of the present invention and are not intended to limit the meaning of these terms or the scope described in the patent claims.

Anyone can easily understand that the present invention can be variously changed, modified, and revised to facilitate implementation of the invention at the level of a person having ordinary skill in the art, within the scope that does not depart from the spirit and scope of the invention as indicated by the claims according to the above-described embodiments.

1; Automated human-derived material extraction device 10; Working container
20; Reagent container 30; Disposable tip
100; Container storage part 110; 1st rack part
120; 2nd rack section 130; 3rd rack section
140; Cooling unit 141; First cooling module
142; Second cooling module 150; Heating unit
200; Fluid transfer member 210; First transfer section
220; Dispensing module 221; Syringe
230; Drop catcher 300; Container transfer member
310; 2nd transfer unit 320; gripper
400; Extraction Absence 410; 1st Working Table
420; 2nd work platform 430; 3rd transfer unit
440; Extraction tip 450; Magnet bar

Claims (13)

A container storage member (100) for storing a work container (10), a reagent container (20), and a disposable tip (30);
A dispensing module (220) equipped with a plurality of syringes (221) for suction and dispensing a target material is provided, and a fluid transport member (200) for transporting the dispensing module (220) in the X, Y, and Z directions;
A container transfer member (300) having a gripper (320) for transferring a work container (10) and allowing the gripper (320) to be transferred in the X, Y, and Z directions;
An automated device for extracting human-derived substances, characterized in that it comprises an extraction member (400) that mixes the sample and the reagent within the settled working vessel (10) and extracts human-derived substances from the mixed sample and reagent when a working vessel (10) into which a reagent is dispensed to a sample through the fluid transfer member (200) is settled through the vessel transfer member (300). In the first paragraph,
The above container storage member (100) comprises a first rack section (110) in which a plurality of work containers (10) are stored in sequence;
A second rack section (120) in which a number of disposable tips (30) according to capacity are stored; and
An automated device for extracting human-derived substances, further comprising a third rack section (130) in which a number of reagent containers (20) are stored for storage at room temperature. In the first paragraph,
The above container storage member (100) is an automated device for extracting human-derived substances, which further includes a cooling unit (140) that cools the working container (10) and the reagent container (20). In the third paragraph,
The above cooling unit (140) has a first cooling module (141) in which a plurality of reagent containers (20) according to capacity are respectively installed and performs cooling of the reagent containers (20); and
An automated device for extracting human-derived substances, wherein a work vessel (10) is installed and further includes a second cooling module (142) that performs cooling of the work vessel (10). In the first paragraph,
The above container storage member (100) is an automated device for extracting human-derived substances, which further includes a heating unit (150) for heating the working container (10). In the first paragraph,
The above fluid transport member (200) comprises a first transport section (210) that transports the dispensing module (220) in the X, Y, and Z directions;
An automated device for extracting human-derived substances, further comprising a dispensing module (220) equipped with a plurality of syringes (221), each equipped with a disposable tip (30) for each capacity at the bottom, which are transported in the X, Y, and Z directions through the first transport section (210). In Article 6,
The above fluid transfer member (200) is provided at the bottom of a plurality of syringes (221), and is capable of horizontally reciprocating toward the side where the disposable tips (30) for each capacity are respectively provided, and an automatic device for extracting human-derived substances further includes a drop catcher (230) that receives substances flowing out from the disposable tips (30). In the first paragraph,
The above container transport member (300) comprises a second transport member (310) that transports the gripper (320) in the X, Y, and Z directions;
An automated device for extracting human-derived materials, which is transported in the X, Y, and Z directions through the second transport unit (310) and further includes a gripper (320) capable of selectively gripping a work container (10). In the first paragraph,
The above extraction member (400) is composed of first and second work platforms (410, 420) on which work containers (10) for different purposes are respectively installed;
It further includes a third transfer unit (430) that moves the first and second work platforms (410, 420) to the target location;
An automated device for extracting human-derived materials, characterized in that a sample and a reagent are mixed in a work container (10) of the first work table (410), a human-derived material is extracted from the mixed sample and reagent, and the extracted human-derived material is transferred to a work container (10) of the second work table (420). In Article 9,
The above extraction member (400) is an extraction tip (440) that mixes a sample and a reagent by moving up and down for a set time while inserted into the inside of a working container (10);
An automated device for extracting human-derived materials, further comprising a magnet bar (450) which is vertically positioned on top of the extraction tip (440), can be inserted into the interior of the extraction tip (440) through an up-and-down operation, and extracts human-derived materials according to the application of a magnetic field. In a method for extracting human-derived materials using an automated human-derived material extraction device according to any one of claims 1 to 10,
(S1) A step of sucking the reagent from the corresponding reagent container (20) stored in the container storage member (100) using the dispensing module (220) of the fluid transfer member (200) and dispensing it to the working container (10) corresponding to the order of the first rack section (110);
(S2) A step of moving the working container (10) in which the reagent is dispensed to the first working table (410) of the extraction member (400) using the container transfer member (300);
(S3) A step of lowering the extraction tip (440) into the work container (10) of the first work table (410) and then mixing the sample and reagent by moving it up and down for a set time;
(S4) A step of moving a work container (10) corresponding to the order of the first rack section (110) of the container storage member (100) to the second work table (420) of the extraction member (400) using the container transfer member (300);
(S5) A step of lowering the extraction tip (440) to the bottom of the work container (10) of the first work table (410);
(S6) A step of lowering the magnet bar (450) so that it is inserted into the extraction tip (440) and then applying a magnetic field to attach the human-derived material to the outside of the extraction tip (440);
(S7) A step of simultaneously raising the extraction tip (440) and the magnet bar (450) to extract human-derived material from the work container (10) of the first work table (410);
(S8) A step of moving the work container (10) of the second work table (420) to the position of the extraction tip (440) and the magnet bar (450) from which the human-derived material is extracted;
(S9) A step of lowering the extraction tip (440) and the magnet bar (450) to the bottom of the working container (10) of the second working table (420);
(S10) A step of raising the magnet bar (450) so as to separate it from the extraction tip (440);
(S11) A step of moving the extraction tip (440) up and down to cause the human-derived material attached to the extraction tip (440) to fall into the working container (10);
(S12) A step of completing extraction by lifting the extraction tip (440) away from the working container (10);
(S13) A method for extracting human-derived substances, characterized by comprising the step of sequentially moving the work container (10) of the first work table (410) and the work container (10) of the second work table (420) in the order corresponding to the first rack section (110) of the container storage member (100). In Article 11,
The above step (S3) is:
(S3-1) A method for extracting human-derived substances further comprising a step of cooling a working container (10) in which mixing of a sample and a reagent is completed using a container transport member (300) using a cooling unit (140) of a container storage member (100), or heating it using a heating unit (150). In Article 11,
The above human-derived material extraction method further includes optionally repeating steps (S1) to (S13) an arbitrary number of times according to the order of the working containers (10) stored in the first rack section (110) of the container storage member (100).