KR20240120405A - Cosmetic composition comprising extract of above-ground part of ginseng - Google Patents

Abstract

The cosmetic composition for preventing skin aging and whitening the skin of the present invention contains extract of ginseng aerial parts as an active ingredient.

Description

Cosmetic composition comprising extract of above-ground part of ginseng}

The present invention relates to a cosmetic composition containing an extract of the aerial parts of ginseng. Specifically, it is a natural cosmetic composition using the underused aerial parts of ginseng, and is a cosmetic composition that can provide excellent functionality for the skin, such as preventing skin aging and whitening the skin. It's about.

Ginseng is an herbal medicine ingredient well known for its excellent pharmacological effects, and is known for its effects such as fatigue recovery, blood flow improvement, immunity strengthening, antioxidant, and memory improvement. However, since the root part of ginseng is mainly used, the remaining part, that is, the above-ground part, is mostly discarded. Since the amount of waste disposed of in this way is also significant, environmental pollution problems may occur as a result. Therefore, in order to solve these problems and increase the income generation of farmers, it is necessary to develop technology that utilizes the aerial parts of ginseng, a ginseng by-product.

Meanwhile, as consumers' interest in well-being increases, the number of customers looking for products using natural ingredients is increasing not only in Korea but also in the global cosmetics market. In particular, this craze for consumption of natural cosmetics is increasing with the implementation of the labeling system for all cosmetic ingredients and the announcement of research results showing that chemical ingredients such as parabens, mineral oil, artificial colors, and formaldehyde are harmful to the skin. In response to these consumer demands, various natural cosmetics are being developed based on research results on the physiological activity of natural products. However, their effects have not yet met consumers' expectations, so the development of new natural cosmetics is necessary.

Accordingly, the present inventors attempted to develop natural cosmetics that could provide excellent functionality to the skin by actively utilizing ginseng aerial parts, which are discarded ginseng by-products.

Korean Patent No. 10-0361433

The main purpose of the present invention is to provide a cosmetic composition that can provide excellent functionality to the skin by utilizing the aerial part, which is a by-product of ginseng, which is mostly discarded.

In one aspect, the present invention provides a cosmetic composition for preventing skin aging and whitening the skin containing an extract of aerial parts of ginseng as an active ingredient.

The cosmetic composition of the present invention is a natural cosmetic composition using ginseng aerial parts, which are mostly discarded ginseng by-products, and can provide excellent functionality for the skin, such as preventing skin aging and whitening the skin. As a result, it is expected that it will be able to meet the demands of modern consumers for cosmetics, improve environmental pollution problems caused by the disposal of ginseng by-products, and provide opportunities for additional profit generation for growers.

Figure 1 shows an HPLC chromatogram for ginsenosides Re, Rg1, and Rb1 of a ginseng aerial part extract (ginseng aerial part hot water extract) according to an embodiment of the present invention.
Figure 2 shows an HPLC chromatogram for ginsenosides Re, Rg1, and Rb1 of a ginseng aerial part extract (70% ethanol extract of ginseng aerial part) according to an embodiment of the present invention.
Figure 3 shows the DPPH test results of an extract of aerial parts of ginseng according to an embodiment of the present invention. Hot water extraction, hot water extract of ginseng aerial parts; 70%EtOH extraction, 70% ethanol extract of ginseng aerial parts; Vitamin C, Vitamin C (positive control).
Figure 4 shows the results of a tyrosinase activity inhibition test of an extract of aerial parts of ginseng according to an embodiment of the present invention. Hot water extraction, hot water extract of ginseng aerial parts; 70%EtOH extraction, 70% ethanol extract of ginseng aerial parts; Arbutin, arbutin (positive control); Vitamin C, Vitamin C (positive control).
Figures 5 and 6 show a survey form on the feeling of using a cosmetic composition.
Figures 7 and 8 show the results of a survey on the feeling of use of the cosmetic composition.

The present invention relates to a cosmetic composition for preventing skin aging and whitening the skin containing an extract of aerial parts of ginseng as an active ingredient.

In the present invention, the above-ground part of ginseng refers to the part exposed to the ground other than the roots of plants of the genus ginseng, for example, ginseng ( Panax ginseng ), American ginseng ( Panax quinquefolium ), Panax notoginseng ( Panax notoginseng ), and bamboo shoots ( Panax japonicum ). ), ginseng leaves, stems, or a mixture thereof selected from the group consisting of trifolium ginseng ( Panax trifolium ) and Himalayan ginseng ( Panax pseudoginseng ).

In the present invention, the extract may be an extract obtained by extracting the extraction raw material with a solvent, and may be obtained using a conventional extraction method using a solvent or an extraction device.

In the present invention, various solvents known in the art can be used as the extraction solvent. Solvents usable in the present invention may be water, alcohols having 1 to 4 carbon atoms, acetone, hexane, ethyl acetate, carbon dioxide, or a mixture of two or more of these. However, for the effect aimed at in the present invention, the solvent is preferred. Water, ethanol, or a mixed solution thereof is used, more preferably 50 to 90% (v/v) ethanol aqueous solution, more preferably 60 to 80% (v/v) ethanol aqueous solution, more preferably uses a 65 to 75% (v/v) ethanol aqueous solution.

The aerial part of ginseng used as an extraction raw material in the present invention can be applied in various forms. For example, it can be applied in the form of pulverized material, and the pulverized product can be obtained through a conventional method of pulverizing raw materials. For example, it can be used by grinding it to a size of 10 mesh or less, 30 mesh or less, or 45 mesh or less.

In the present invention, the extraction method may use various methods known in the art, for example, adding an extraction solvent to the extraction raw material and performing extraction by standing, shaking, or ultrasonic extraction. For the effect aimed at in the present invention, preferably, 1 to 100 times (w/v) of extraction solvent is added based on the weight of the aerial part of ginseng, which is the extraction raw material, and more preferably, based on the weight of the aerial part of ginseng. 5 to 50 times (w/v) of the extraction solvent is added, more preferably, 10 to 30 times (w/v) of the extraction solvent is added based on the weight of the above-ground part of ginseng, and more preferably, the amount of extraction solvent is added based on the weight of the above-ground part of ginseng. Add 15 to 25 times (w/v) of extraction solvent by weight. Also, at this time, the extraction temperature is preferably 0 to 100°C, more preferably 10 to 80°C, more preferably 20 to 60°C, and more preferably 20 to 60°C for the effect aimed at in the present invention. It is set at 30 to 50℃. At this time, the extraction time is preferably 10 minutes to 24 hours, more preferably 30 minutes to 10 hours, and more preferably 40 minutes to 2 hours for the effect targeted by the present invention. In addition, extraction is repeated several times, that is, first extraction is performed, followed by filtration to obtain an extract, adding extraction solvent again to the extraction residue, and performing a second extraction, and collecting the extracts obtained in each order. You can use it. At this time, the 2nd to 4th repeated extraction would be appropriate. For the effect targeted by the present invention, ultrasonic extraction is preferably used. At this time, the ultrasonic wave is preferably 10 to 100 kHz, more preferably 20 to 90 kHz, more preferably 30 to 80 kHz, more preferably 40 to 70 kHz, and even more preferably 50 to 60 kHz.

After extraction using the above solvent, the extract is preferably filtered before use. Additionally, it can be used by filtration, concentration under reduced pressure, and freeze-drying after concentration under reduced pressure.

The cosmetic composition of the present invention may be composed solely of the ginseng aerial part extract of the present invention, and may further include a cosmetically acceptable carrier or additive. At this time, the carrier also includes pharmaceutically acceptable carriers. Additionally, the cosmetic composition of the present invention can be formulated into various forms, such as body lotion, moisturizing cream, lotion, nutritional lotion, nutritional cream, massage cream, pack, and nutritional essence.

In a preferred embodiment for the effect targeted by the present invention, the cosmetic composition of the present invention contains 0.5% by weight or more of the ginseng aerial part extract, more preferably 1% by weight or more.

In a preferred embodiment for the effect targeted by the present invention, the cosmetic composition of the present invention contains an extract of ginseng aerial parts together with purified water; glycerin; sodium hyaluronate; 1,3-butylene glycol; titanium dioxide; squalene; shea butter; Montawax (e.g. Montanov-L); methyl-p-hydroxybenzoate (eg, danisol-M); propyl-p-hydroxybenzoate (eg, danisol-P); glyceryl stearate and PEG-100 stearate mixed surfactant (eg, Arlacel 165); butylene glycol dicaprylate/dicaprate (e.g., dermofeel BGC); triethanolamine; carbopol; and cyclopentasiloxane (eg, DC-245); and is formulated as a body lotion.

The above additional ingredients are well known ingredients used in cosmetics.

For the effect aimed at in the present invention, the cosmetic composition of the present invention preferably contains 0.5 to 10% by weight of ginseng aerial part extract, 65 to 90% by weight of purified water, and 0.5 to 10% by weight of glycerin. % by weight, sodium hyaluronate at 0.0005 to 0.05% by weight, 1,3-butylene glycol at 0.1 to 8% by weight, titanium dioxide at 0.1 to 8% by weight, and squalene at 0.5% by weight. to 10% by weight, shea butter at 0.01 to 4% by weight, Montawax at 0.05 to 5% by weight, methyl-p-hydroxybenzoate at 0.005 to 0.5% by weight, It contains 0.001 to 0.4% by weight of propyl-p-hydroxybenzoate, 0.01 to 4% by weight of a mixed surfactant of glyceryl stearate and PEG-100 stearate, and butylene glycol dicaprylate/ It contains 0.1 to 8% by weight of dicaprate, 0.05 to 5% by weight of triethanolamine, 0.005 to 3% by weight of carbopol, and 0.5 to 10% by weight of cyclopentasiloxane. More preferably, it contains 1 to 5% by weight of ginseng aerial part extract, 70 to 85% by weight of purified water, 1 to 5% by weight of glycerin, and 0.001 to 0.03% by weight of sodium hyaluronate. Contains 1,3-butylene glycol at 0.5 to 4% by weight, titanium dioxide at 0.5 to 4% by weight, squalene at 1 to 5% by weight, and shea butter at 0.05 to 2% by weight. %, Montawax is included at 0.1 to 3% by weight, methyl-p-hydroxybenzoate is included at 0.01 to 0.3% by weight, and propyl-p-hydroxybenzoate is included at 0.005 to 0.2% by weight. It contains 0.05 to 2% by weight of a mixed surfactant of glyceryl stearate and PEG-100 stearate, 0.5 to 4% by weight of butylene glycol dicaprylate/dicaprate, and triethanolamine. It contains 0.1 to 3% by weight, carbopol at 0.02 to 2% by weight, and cyclopentasiloxane at 1 to 5% by weight.

The cosmetic composition of the present invention may further include other functional substances in addition to the ginseng aerial part extract of the present invention.

Hereinafter, the present invention will be described in more detail through examples. Since these examples are merely for illustrating the present invention, the scope of the present invention is not to be construed as limited by these examples.

[Example]

Example 1. Preparation of cosmetic composition

1-1. Manufacture of ginseng aerial part extract

Except for the roots of ginseng, the remaining aerial parts (leaves and stems) were used as extraction raw materials, and distilled water or 70% (v/v) ethanol solution (mixed solution of water and ethanol) was used as the extraction solvent.

First, the extracted raw materials were freeze-dried for 3 days and pulverized in a blender. 20 times (w/v) of extraction solvent was added to 20 g of the freeze-dried pulverized material for extraction and ultrasonic extraction was performed. Ultrasonic extraction was performed at 40°C and 53 kHz for 60 minutes. Afterwards, the extract was filtered first using a cotton cloth, second filtered with filter paper (150 mm) to obtain an extract, and concentrated under reduced pressure.

1-2. Cosmetic composition manufacturing

Using the ginseng aerial part extract of 1-1 above, body lotion was prepared with the composition shown in Table 1 below.

ingredient weight% awards department Purified water 64.85 glycerin 3 Sodium Hyaluronate (1%) One 1,3-butylene glycol 2 paid part titanium dioxide 2 Squalane 3 shea butter 0.5 Montawax L One Danisol-M 0.1 Danisol-P 0.05 Aracel 165 0.5 Dermofil BGC 2 thickener Triethanolamine One Carbopol (2%) 13 DC-245 3 Ginseng aerial part extract 3 Sum 100

* ‘Sodium hyaluronate (1%)’ refers to a 1% (w/v) aqueous solution of sodium hyaluronate, and ‘Carbopol (2%)’ refers to a 2% (w/v) aqueous solution of Carbopol.

Specifically, the aqueous phase of Table 1 was mixed, heated and dissolved to 75°C, and then cooled to 50°C. The water phase was mixed at 5,000 rpm for 3 minutes using a homomixer. The oil phase in Table 1 was mixed and dissolved by heating to 75°C and then cooled to 50°C. The oil phase was added to the water phase while emulsifying with a homomixer. Afterwards, the thickener was mixed and added. It was emulsified for 2 minutes at 5,000 rpm using a homomixer. Afterwards, the ginseng aerial part extract of 1-1 was added and emulsified at 2,000 rpm for 2 minutes, then cooled to 30°C and stored.

Experimental Example 1. Ingredient analysis

Ginsenoside Re, Rg1, and Rb1 components of the ginseng aerial part extract of 1-1 were analyzed. The analysis was conducted by requesting the Jeonju Agricultural and Life Materials Research Institute, a foundation, and the sample pretreatment and analysis conditions were as follows.

○ Pretreatment method

50 mg of the concentrate sample was taken and diluted with 50% methanol aqueous solution, vortexed and centrifuged, and the supernatant was taken and used as an HPLC sample solution.

○ Analysis conditions

- HPLC: Agilent 1200 HPLC

- Detection: Multiwavelength detector 210nm

- Column: Aegispak-L C18 (4.6x200mm ID, 3 micro pore size)

- Column temp. : 35dg.

- Flow rate: 0.38 ml/min

- Injection: 15㎕

- Elution: water / acetonitrile as the gradient

As a result of the analysis, in the case of the hot water extract of the aerial part of ginseng, the content of ginsenoside Re was 44.04 mg/g, the content of Rg1 was 24.31 mg/g, and the content of Rb1 was 1.60 mg/g, and in the case of the 70% ethanol extract of the aerial part of ginseng, The content of ginsenoside Re was found to be 44.61 mg/g, the content of Rg1 was 24.36 mg/g, and the content of Rb1 was 7.22 mg/g (Figures 1 and 2).

These results show that the extract of the aerial part of ginseng, which is a by-product of ginseng, also contains a large amount of ginsenoside compounds, and in particular, the 70% ethanol extract can contain ginsenoside Rb1 at a higher concentration.

Experimental Example 2. DPPH test

The antioxidant effect of ginseng aerial part extract was investigated through the DPPH test. This test measures antioxidant activity based on the fact that the purple DPPH radical changes to light yellow when it reacts with an antioxidant sample. At an absorbance wavelength of 517 nm, purple is detected and yellow is not detected, so the absorbance value can be obtained.

The ginseng aerial part extract of Example 1-1 above was used as a test sample.

Test samples with concentrations of 5 mg/ml, 2.5 mg/ml, and 1.25 mg/ml were investigated, and vitamin C (100 μg/ml, 50 μg/ml, 10 μg/ml) was used as a positive control group. used. DPPH was mixed with samples and positive controls at each concentration, reacted at 37°C for 30 minutes, then measured absorbance at 517 nm, and DPPH radical scavenging ability was calculated according to the formula below.

[formula]

DPPH radical scavenging ability (%) = 100 - (absorbance of group with sample added/absorbance of group without sample) * 100

As a result of the test, the hot water extract of the aerial part of ginseng showed a DPPH radical scavenging activity of 62.9592%, 55.1880%, and 49.3488% at concentrations of 5 mg/ml, 2.5 mg/ml, and 1.25 mg/ml, respectively, and the DPPH radical scavenging ability of the 70% ethanol extract of the aerial part of ginseng was In this case, the DPPH radical scavenging ability was 69.4075%, 62.6454%, and 58.0614% at concentrations of 5 mg/ml, 2.5 mg/ml, and 1.25 mg/ml, respectively (Figure 3).

These results show that the cosmetic composition of the present invention has an excellent antioxidant effect and, based on this, can exhibit an excellent skin aging prevention (anti-skin aging) effect.

Experimental Example 3. Tyrosinase activity inhibition test

The skin whitening effect of ginseng aerial part extract was investigated through a tyrosinase activity inhibition test. This test is a method to evaluate the skin whitening effect by measuring the activity inhibition of the DOPA oxidation reaction of tyrosinase, which is involved in the rate-determining step of the melanin synthesis process. L-DOPA (L-3,4-dihydroxyphenylalanine) is used as a substrate. do. A sample with good effect has a small absorbance value, and a sample with a poor effect has a large absorbance value.

The ginseng aerial part extract of Example 1-1 was used as a test sample.

The case where the test sample had a concentration of 1.25㎎/㎖ was investigated, and arbutin (100㎍/㎖) and vitamin C (100㎍/㎖) were used as positive controls. Phosphate buffer solution (pH 7.0), sample solution, and tyrosinase solution (1000 units) were sequentially added to the test tube, reacted in a constant temperature water bath at 37°C for 20 minutes, and absorbance was measured at 475 nm.

As a result of the test, in the case of a hot water extract of ginseng aerial parts, the absorbance was measured at 0.1759 at a concentration of 1.25 mg/ml, and in the case of a 70% ethanol extract of ginseng aerial parts, the absorbance was measured at 0.1105 at a concentration of 1.25 mg/ml (Figure 4).

These results show that the cosmetic composition of the present invention has an excellent tyrosinase activity inhibition effect and, based on this, can exhibit an excellent skin whitening effect.

Experimental Example 4. Cosmetic composition usage survey

A survey was conducted on the feeling of use of the cosmetic composition (body lotion formulation) of Example 1. At this time, the survey form used is the same as Figures 5 and 6.

As a result of the survey, subjects with skin-related conditions as shown in Figure 7 responded to the survey, and as shown in Figure 8, both body lotions using hot water extract and 70% ethanol extract were comfortable to use, and the skin was soft and moist after use. There were many opinions that there was a feeling, and many said that the skin did not feel itchy, tight, or stinging when using it. There were many opinions that body lotion using hot water extract of ginseng aerial parts had a better scent than body lotion using 70% ethanol extract.

Claims (3)

A cosmetic composition for preventing skin aging and whitening the skin containing extract of the aerial parts of ginseng as an active ingredient. According to paragraph 1,
A cosmetic composition for preventing skin aging and whitening the skin, wherein the ginseng aerial part extract is an extract of a solvent selected from the group consisting of water, alcohol, and a mixed solution thereof. According to paragraph 1,
Purified water, glycerin, sodium hyaluronate, 1,3-butylene glycol, titanium dioxide, squalane, shea butter, montawax, methyl-p-hydroxybenzoate, propyl-p-hydroxybenzoate, glyceryl stearate and Cosmetic for anti-aging and skin whitening formulated as a body lotion, further comprising a mixed surfactant of PEG-100 stearate, butylene glycol dicaprylate/dicaprate, triethanolamine, carbopol, and cyclopentasiloxane. Composition.