KR20220027785A - 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스감염증 예방 또는 치료용 백신 - Google Patents

Abstract

본 발명은 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신에 관한 것이다. 본 발명의 코로나바이러스 재조합 스파이크 단백질은 안정하여 세포 내에서 잘 분해가 되지 않고, 면역세포를 효과적으로 활성화시켜 항체 생성량 및 T 세포 반응성이 높고, 본 발명의 벡터는 항원 발현도가 높게 나타남에 따라, 항체 생성량 및 T 세포 반응성이 높고, 항체 생성 발현 기간이 길었으며, 간 독성을 보이지 않음이 확인되었으므로, 코로나바이러스 감염증 예방 또는 치료용 백신으로 유용하게 사용될 수 있다.

Description

신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스감염증 예방 또는 치료용 백신{Novel coronavirus recombinant spike protein, polynucleotide encoding the protein, vector comprising the polynucleotide, and vaccine for preventing or treating coronavirus infection comprising the vector}

본 발명은 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스감염증 예방 또는 치료용 백신에 관한 것이다.

심각한 호흡기 질환을 유발하여 사망을 초래하는 코로나바이러스감염증은 Coronaviridae에 속하는 RNA 바이러스로 분류되며, 코로나바이러스 감염에 의한 호흡기 증후군으로 정의된다. Coronaviridae에 속하는 바이러스 중 사람에게 감염을 일으키다고 알려진 바이러스는 총 7종으로 감기를 일으키는 4종(229E, OC43, NL63, HKU1), 중증 폐렴을 일으키는 2종(SARS-CoV, MERS-CoV) 및 이번 팬데믹 사태의 원인 바이러스인 SARS-CoV-2가 있다. 중증 폐렴을 일으키는 3종의 바이러스(SARS-CoV, MERS-CoV 및 SARS-CoV-2)는 2002년 SARS-CoV를 시작으로 2012년 MERS-CoV에 이어 SARS-CoV와 유전자 서열의 상동성이 높은 현재의 SARS-CoV-2까지 전세계적 대유행이 진행되고 있다.

코로나 바이러스(Coronaviridae)는 불안정한 RNA를 유전체로 가지고 있어 돌연변이가 쉽게 발생하고, 이러한 특성으로 인해 동물과 사람 모두에서 전염될 가능성이 있다. 현재까지 연구에 따르면, SARS-CoV는 사향 고양이에서 인간으로, MERS-CoV는 단봉 낙타에서 인간으로 전염되었으며, 이러한 사례를 미루어 보아 동물을 숙주로 하는 다른 종의 코로나바이러스는 인간에게 감염을 일으키는 변종 바이러스로 진화할 수 있는 잠재성을 가지고 있다.

SARS-CoV-2는 2019년 후반에 최초 보고되었다. 2002년 중국에서 발생한 SARS-CoV와 비교했을 때 중증도는 SARS-CoV가 높지만 전파력은 SARS-CoV-2가 월등히 높은데, 이는 SARS-CoV-2의 세포 수용체 결합부위인 스파이크 단백질의 돌연변이에 의한 것으로 분석하고 있다. 이러한 결과로 전세계적인 대유행을 초래하게 되었다. 감염의 일반적인 징후로는 호흡기 증상, 발열, 기침, 호흡 곤란 및 호흡 곤란이 있다. 더 심한 경우 감염은 폐렴, 심각한 급성 호흡기 증후군, 신부전 및 심지어 사망을 유발할 수 있다.

현재 수많은 국가와 기관에서 COVID-19 팬데믹 사태를 근절하기 위한 백신 개발에 진력하고 있다. 예방백신의 종류는 제작 방법에 따라 사백신(inactivated vaccine), 약독화 생백신(attenuated vaccine), 단백질 서브유닛 백신(subunit vaccine), 바이러스 벡터 기반 백신(viral vector based vaccine), DNA 백신, mRNA 백신과 같이 분류된다. 전통적으로 백신 제형으로 많이 이용되어 왔던 사백신(inactivated vaccine)과 약독화 생백신(attenuated live vaccine)은 제작과정이 간단한 장점이 있지만, 사백신의 경우 높은 수준의 면역반응을 유도하지 못하고, 약독화 생백신은 돌연변이를 통해 병원성을 다시 획득할 수 있는 위험성이 존재한다. 단백질 서브유닛 백신(Subunit vaccine)은 면역 반응을 잘 유도하지 못하기 때문에 면역증강제를 함께 사용해야 하는 문제점이 있다.

생명공학 기술의 발전에 따라 새로운 플랫폼의 백신 제형이 개발되기 시작했는데, 그 중 하나가 바이러스 벡터 기반 백신(viral vector based vaccine)이다. 벡터 기반 백신은 인체 세포에 백신 항원 유전자를 높은 효율로 전달하여 항원 단백질을 체내에서 스스로 생성하게 하여 면역반응을 활성화한다. 바이러스 벡터 기반 백신은 안전하며, 높은 수준의 면역반응을 유도하기 때문에 반복 투여가 필요없이 단회 투여만으로 효율적인 면역화가 가능하다. 또한, 구조적으로 바이러스에 기반 하여 항체 생성뿐 아니라 세포독성 T 세포 면역을 효율적으로 유도할 수 있는 장점이 있다. 많은 바이러스가 유전자 벡터로서 연구가 되고 있으며, 그 중 아데노바이러스(Adenovirus)는 많은 연구가 이루어져 조작이 용이하고 안전성이 검증되어 유전자 치료분야에서 많이 사용되고 있으며, 비교적 크기가 큰 항원(9~35kb)을 전달할 수 있기 때문에 다른 바이러스 벡터와 비교하여 높은 경쟁력을 가지고 있다.

아데노바이러스는 선형, 이중 가닥의 DNA 바이러스로, 직경 70-90nm이며, 외피가 없고, 이십면체형 캡시드를 가지며, 이는 각 20면체의 꼭짓점에서 연장되는 240 헥손, 12 펜톤 및 섬유에 의해 형성된다. 이들 헥손, 펜톤 및 섬유는 주류 아데노바이러스 항원 및 이의 혈청형을 결정한다. 아데노바이러스 게놈은 크기가 약 30-45kb이며 4개의 초기 영역 (E1, E2, E3 및 E4) 및 5개의 후기 영역 (L1-L5)을 갖는다. 아데노바이러스는 감염성이 높고 그들 중 다수는 병원성이 없기 때문에 백신 생산을 위한 재조합 벡터로서 좋은 후보이다. 또한, 아데노바이러스 벡터는 대형 유전자를 효율적으로 번역할 수 있으며, 동물에서 면역 반응을 연장 시킬 수 있다. 아데노바이러스 벡터는 자가복제를 담당하는 E1 유전자를 제거한 형태로 주로 이용되며, E1 유전자가 제거된 아데노바이러스 벡터는 인체 세포에서는 자가 복제가 불가능하기 때문에 체내에서 병원성을 유발하지 않아 안전하다.

Ad5/35 벡터는 복제불능 아데노바이러스 혈청형 5(Ad5)의 돌기(knob) 유전자가 아데노바이러스 혈청형 35(Ad35)의 돌기 유전자로 치환된 형태이다. 이러한 변형을 통해 Ad5/35 벡터는 기존 Ad5의 세포 수용체가 아닌 인간 면역세포에 높게 발현하는 CD46 수용체를 통해 세포 내로 도입될 수 있게 된다. 즉, Ad5/35는 인간의 항원제시세포로 효율적으로 도입되어, 항원의 생산을 극대화할 수 있는 효율적인 백신 플랫폼이 될 수 있다. 또한, Ad5/35 벡터는 Ad5 벡터와 비교했을 때, 간 독성을 유발할 위험성이 현저히 낮다는 것이 보고된 바 있다. 실제로 본 발명자들은 아데노바이러스 벡터를 이용해 암 환자에게 암 항원 유전자를 전달하면 높은 수준의 면역반응이 유도됨을 전임상 및 임상시험에서 확인한 바 있으며, 임상 시험에서 다수의 환자들에게 투여되었을 때 안전성 및 독성과 관련한 문제도 발생하지 않음을 확인하였다.

백신 후보물질을 제작할 때의 고려사항은, 발현되는 항원의 안정성 (stability)이다. 백신 항원은 다량 분비되는 것도 중요하지만, 발현되더라도 초기에 빠르게 분해되면, 면역세포를 효과적으로 활성화 시키지 못할 우려가 있다. 이에 항원을 장기간 안정적으로 유지시키는 것이 중요하다.

이에, 본 발명자들은 코로나바이러스감염증-19(COVID-19) 예방 또는 치료용 백신을 개발하기 위하여, SARS-CoV-2의 세포 감염에 핵심적인 역할을 하는 스파이크 단백질(Spike protein)을 코딩하는 S1 유전자와 S2 사이에 위치한 절단 인지 서열(Cleavage site)을 링커 서열로 치환한 폴리뉴클레오티드를 벡터에 도입하여 예방 또는 치료용 백신을 제작하였고, 상기 백신이 SARS-CoV-2에 대한 항체 생성량 및 T 세포 반응성이 높음을 확인하여 본 발명을 완성하였다.

본 발명의 목적은 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스감염증 예방 또는 치료용 백신을 제공하는 것이다.

상기 목적을 달성하기 위해, 본 발명은 코로나바이러스 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 포함하는 벡터를 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 포함하는 벡터를 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 아데노바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자의 폴리뉴클레오티드를 포함하는 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자의 폴리뉴클레오티드를 포함하는 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자의 폴리뉴클레오티드를 아데노바이러스에 도입한 것을 특징으로 하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자의 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 벡터를 포함하는 코로나바이러스 감염증-19(COVID-19) 예방 또는 치료용 백신을 제공한다.

본 발명은 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신에 관한 것이다. 본 발명의 코로나바이러스 재조합 스파이크 단백질은 안정하여 세포 내에서 잘 분해가 되지 않고, 면역세포를 효과적으로 활성화시켜 항체 생성량 및 T 세포 반응성이 높고, 본 발명의 벡터는 항원 발현도가 높게 나타남에 따라, 항체 생성량 및 T 세포 반응성이 높고, 항체 생성 발현 기간이 길었으며, 간 독성을 보이지 않음이 확인되었으므로, 코로나바이러스 감염증 예방 또는 치료용 백신으로 유용하게 사용될 수 있다.

도 1은 아데노바이러스 혈청형 5(Adenovirus serotype 5)를 기반으로, 수용체 결합부위인 E3 유전자 영역을 아데노바이러스 혈청형 35(Adenovirus serotype 35)의 파이버(fiber) 유전자로 치환하고, 여기에 SARS-CoV-2의 세포 감염에 핵심적인 역할을 하는 스파이크 단백질을 항원으로 탑재한 본 발명에 따른 코로나바이러스감염증-19 예방 또는 치료용 백신을 나타낸 모식도이다.
도 2는 본 발명의 코로나바이러스감염증-19 예방 또는 치료 백신이 항원으로서 스파이크 단백질을 코딩하는 유전자의 절단 인지 서열을 링커 서열로 치환한 재조합 스파이크 단백질이 구조의 안정성 및 항원 발현도가 높고, 항체 생성 B세포에 중화 항체를 생산하는 능력이 뛰어남을 나타낸 모식도이다.
도 3은 본 발명의 코로나바이러스감염증-19 예방 또는 치료용 백신의 작용 기전을 나타낸 모식도이다.
도 4는 아데노바이러스 혈청형 5(Adenovirus type 5, Ad5)의 유전체를 사용하여 제작한 대조군 발현 벡터를 나타난 모식도이다.
도 5는 항원 발현 유전자인 스파이크 단백질 항원을 탑재한 아데노바이러스 #1 내지 # 4 벡터를 나타낸 모식도이다.
도 6은 항원 발현 유전자인 스파이크 단백질 항원을 탑재한 아데노바이러스 #1 내지 # 4 벡터를 배양 세포주에 도입하였을 때 항원 발현도를 나타낸 도이다.
도 7은 항원 발현 유전자인 스파이크 단백질 항원을 탑재한 아데노바이러스 #1 내지 # 4 벡터를 배양 세포주에 도입하였을 때 세포 밖으로 배출되는 항원 발현도를 나타낸 도이다.
도 8은 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 #1 내지 # 4 벡터를 마우스에 주입 후 항체 생성량을 나타낸 도이다.
도 9는 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 3 벡터를 마우스에 주입 후 혈액 내에 존재하는 항원 특이 항체의 양을 나타낸 도이다.
도 10은 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 3 벡터를 원숭이에 주입 후 항체 생성량을 나타낸 도이다.
도 11은 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 3 벡터를 원숭이에 주입 후 중화 항체 농도를 나타낸 도이다.
도 12는 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 1 내지 # 4 벡터를 원숭이 주입 후 혈중 간 독성 수치를 나타낸 도이다.
도 13은 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 1 내지 # 3 벡터를 마우스에 주입 후 비장 세포 및 말초혈액단핵세포(PBMC)에서 T 세포 반응성을 나타낸 도이다.
도 14는 항원 발현 유전자인 스파이크 항원을 탑재한 아데노바이러스 # 3 벡터를 영장류에 주입 후 말초혈액단핵세포(PBMC)에서 T 세포 반응성을 나타낸 도이다.
도 15는 마우스에 실시예 3에서 제작한 스파이크 항원을 발현하는 #3 벡터 또는 #3 벡터의 아데노바이러스 유전자 중 E4 유전자를 바이러스 유전체의 E1 영역으로 재배치한 벡터를 주입하였을 때, 유사한 수준의 중화 항체를 생산한 것을 확인한 도이다.
도 16은 원숭이에 실시예 3에서 제작한 스파이크 항원을 발현하는 #3 벡터 또는 #3 벡터의 아데노바이러스 유전자 중 E4 유전자를 바이러스 유전체의 E1 영역으로 재배치한 벡터를 주입하였을 때, 유사한 수준의 중화 항체를 생산한 것을 확인한 도이다.

이하, 본 발명을 상세히 설명한다.

본 발명은 코로나바이러스 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 제공한다.

상기 코로나바이러스는 베타 코로나바이러스 속에 속하는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 한다.

상기 SARS-CoV-2와 SARS-CoV의 유전체 상동성은 79.6%, SARS-CoV-2와 MERS-CoV의 유전체 상동성은 50%로 높은 것이 특징이다. 특히, SARS-CoV-2와 MERS-CoV는 상기 발명의 표적인 스파이크 단백질의 상동성이 35%로 유사한 반면, SARS-CoV-2와 SARS-CoV의 스파이크 단백질 상동성은 76%로 매우 높은 것을 특징으로 한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 제공한다.

백신에서 항원은 다량 분비되는 것도 중요하지만, 다량으로 발현되더라도 초기에 분해되면 면역세포를 자극하여 면역반응을 충분히 유도하지 못하기 때문에, 발현된 항원의 안정성이 높은 것이 매우 중요하다. 본 발명의 S1 및 S2 사이에 링커 서열이 연결된 재조합 스파이크 단백질은 항원 발현도가 높을 뿐만 아니라 안정성이 향상되어 중화 항체 생성 능력 및 T 세포 반응성을 유도하는 능력이 뛰어나다.

용어 "면역 반응"은, 이로 한정되는 것은 아니지만, 체액성 면역 반응 및 세포성 면역 반응, 예를 들어, CD4+ 또는 CD8+ 세포 활성화를 포함할 수 있다.

상기 링커 서열로 연결된 스파이크 단백질 항원은 항원 발현도가 증가하는 것을 특징으로 한다.

상기 스파이크 단백질(Spike protein, S protein)은 코로나바이러스 입자의 표면을 덮고 있고, 코로나바이러스가 인체 세포에 침입할 때 이용하는 돌기 모양의 단백질로 S1와 S2의 서브유닛으로 구성되고 인체 세포의 안지오텐신변환효소-2(Angiotensin Converting Enzyme 2, ACE-2) 수용체와 결합한 후 세포 속으로 침투해 유전 물질(RNA)를 밀어 넣어 자신을 복제하는 방식으로 인체를 감염시킨다.

상기 재조합 단백질은 스파이크 단백질의 서브유닛인 S1 및 S2 유전자 사이의 절단 인지 서열(cleavage site)이 제거된 것을 특징으로 한다.

상기 단백질은 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열로 연결되는 것을 특징으로 한다.

상기 링커는 (GGGGS)n으로 구성되는 것을 특징으로 하고, 상기 n은 1 내지 5의 정수이다.

각 단백질을 구성하는 도메인들은 고유의 특성에 따라 상호 작용에 최적화된 거리(링커 서열의 길이)를 가지며, 링커 서열의 길이에 따라 단백질의 구조적 안정성이 발현된다. 따라서, 상기 단백질은 바람직하게는 n은 1 내지 3일 수 있고, 가장 바람직하게는 n은 1이다.

n이 4를 초과하는 경우 반복적인 서열이 다수 발생하게 되어 의도하지 않은 상동 재조합에 의해 링커 서열이 삭제될 수 있을 뿐만 아니라, 단백질의 안정성이 감소 되는 결과를 초래한다.

상기 링커 서열로 연결된 스파이크 단백질 항원은 안정성(stability)이 증가된 것을 특징으로 한다.

상기 링커 서열은 서열번호 19 또는 서열번호 20으로 구성되는 것을 특징으로 한다.

코로나바이러스는 모두 공통적으로 스파이크 단백질을 가지고 있으며, 스파이크 단백질은 S1 및 S2의 서브유닛으로 구성되어 있으므로 코로나바이러스의 모든 스파이크 단백질이 사용 가능하다.

이들 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커로 연결된 스파이크 단백질은 모두 이 발명에 속한다.

코로나 바이러스에는 감기를 일으키는 229E, OC43, NL63, HKU1, 중증 폐렴을 일으키는 SARS-CoV, MERS-CoV, SARS-CoV-2가 있으며, 이들의 스파이크 단백질이 모두 사용 가능하다.

바람직하게는 SARS-CoV-2의 스파이크 단백질의 S1, S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질이다.

상기 재조합 스파이크 단백질은 면역원성을 향상시키기 위하여 어쥬번트(adjuvant)를 추가적으로 포함할 수 있고, 상기 어쥬번트는 GM-CSF, IL-17, IFNNg, IL-15, IL-5, JNK, NFkB, NKKKKLIGAND, PD1/2, NKKKKG2B, NKG2C, NKKKKG2E, NKKKK2F, TAPAPAP2, 및 이의 기능적 단편, E-selectin, IL-α, IL-6, INF-γ, Lympmpmphotoxin α, hGH-1, MIPPP1, IL-7, IL-8, APP, IRARAK, IkB, KILILUX, TRAIL-1, IL-1, AIR, and ICAM-1, ICAM-1, TAP2, CD40의 기능적 단편, TAP1, TRAILrecDRC5, CD34, CD40L, DR3, GlyCAM1, p55, CD2, Ox40, TRAF6, DR4, ICAM3, DR5, MyD88, p65Rel, pl5095, p38, CPG 및 TLR로부터 선택되는 어느 하나 이상일 수 있다.

상기 어쥬번트는 재조합 스파이크 단백질의 C-말단에 연결될 수 있다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 제공한다.

상기 코로나바이러스는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 제공한다.

상기 링커는 9 내지 45개의 염기로 구성되는 것을 특징으로 하고, 바람직하게는 9 내지 21개의 염기로 구성되며, 더 바람직하게는 15개의 염기로 구성된다.

상기 링커 서열로 연결된 스파이크 단백질을 코딩하는 폴리뉴클레오티드는 안정성(stability)이 증가된 것을 특징으로 한다.

상기 링커 서열로 연결된 스파이크 단백질을 코딩하는 폴리뉴클레오티드는 항원 발현도가 증가하는 것을 특징으로 한다.

상기 링커 서열은 서열번호 11 또는 서열번호 15로 구성되는 것을 특징으로 한다.

상기 폴리뉴클레오티드는 서열번호 12 또는 16일 수 있다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 포함하는 벡터를 제공한다.

상기 코로나바이러스는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 포함하는 벡터를 제공한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 아데노바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 아데노바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

상기 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 아데노바이러스에 도입한 벡터는 서열번호 27 또는 29일 수 있다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 벡터를 제공한다.

본 발명에 있어서, "벡터"는 클론 유전자(또는 클론 DNA의 다른 조각)를 탑재하여 표적 세포로 운반하는 전달체를 의미한다.

상기 벡터는 플라스미드는 및 바이러스로 이루어진 군으로부터 선택되는 어느 1종일 수 있다.

플라스미드 DNA의 구체적인 예로는 pCMV3, pET28a 및 pET 같은 상업적인 플라스미드를 포함한다. 본 발명에 사용될 수 있는 플라스미드의 다른 예로는 대장균 유래 플라스미드(pCMV3, pET28a, pET, pGEX, pQE, pDEST 및 pCOLD), 바실러스 서브틸리스(Bacillus subtilis)-유래 플라스미드(pUB110 및 pTP5) 및 효모-유래 플라스미드(YEp13, YEp24 및 YCp50)가 있다. 이러한 플라스미드는 숙주 세포에 따라서 단백질의 발현량과 수식 등이 다르게 나타나므로, 목적에 가장 적합한 숙주 세포를 선택하여 사용하면 된다.

상기 바이러스는 벡터로 사용될 수 있는 공지의 바이러스는 모두 사용할 수 있으며, 아데노바이러스, 레트로바이러스, 렌티바이러스, 아데노 연관 바이러스(Adeno-associated virus, AAV), modified vaccinia virus ankara(MVA), Herpes simplex virus, Baculovirus 중 어느 1종 일 수 있으나, 이에 한정되는 것은 아니다. 본 발명의 구체적인 실시예에 따르면 상기 바이러스는 아데노바이러스인 것이 바람직하다.

본원에서 사용된 용어 "아데노바이러스 벡터"는 아데노바이러스 게놈이 아데노바이러스 게놈과 관련하여 고유하지 않은(non-native) 핵산 서열을 지니도록 조작된 아데노바이러스를 나타낸다. 따라서, 본원에서 사용된 "재조합 아데노바이러스 벡터"는 전형적으로는 아데노바이러스 게놈 및 필요한 단백질(예, SARS-CoV-2의 스파이크단백질 또는 S1 및 S2 사이의 절단 인지 서열을 링커 서열로 치환한 재조합 스파이크 단백질)를 인코딩하는 적어도 하나의 외래 핵산 서열이 포함되어 있는 발현 카세트를 포함한다.

아데노바이러스 벡터는 바람직하게는 바이러스 DNA의 복제를 지지하기에 요구되는 각각의 말단 반복서열의 적어도 일부, 바람직하게는, 적어도 약 90%의 완전 역전된 말단 반복(inverted terminal repeat: ITR) 서열, 및 게놈을 바이러스 캡시드 내로 인캡시드화(encapsidate)하기에 요구되는 DNA를 함유한다. 다양한 기원으로부터의 아데노바이러스가 아데노바이러스 벡터를 위한 바이러스 게놈의 공급원으로서 사용될 수 있다. 인간 아데노바이러스, 예를 들어, 서브그룹 A(예, 혈청형 12, 18 및 31), 서브그룹 B(예, 혈청형 3, 7, 11, 14, 등), 서브그룹 C(예, 혈청형 1, 2, 5, 및 6), 서브그룹 D(예, 혈청형 8, 9, 10, 13, 15, 17, 19, 20, 등), 서브그룹 E(예, 혈청형 4), 서브그룹 F(예, 혈청형 40 및 41) 및 그 밖의 것들이 바람직하다. 바람직하게는, 아데노바이러스 벡터는 인간 서브그룹 C, 특히, 혈청형 2, 더욱더 바람직하게는 혈청형 5의 벡터이다.

아데노바이러스 벡터는 복제 적격 벡터일 수 있다. 전형적으로는, 아데노바이러스 벡터는 숙주 세포에서 복제-결손 벡터이다. 용어 "복제-결손(replication-defective)"은 아데노바이러스 벡터가 복제를 위한 아데노바이러스 게놈의 하나 이상의 유전자 기능 또는 영역(예, E1, E3 또는 E4 영역)에서 결핍을 지녀서 벡터가 어떠한 낮은 수준의 복제를 유지하거나 정상 숙주 세포, 특히, 아데노바이러스 벡터에 의해서 감염되는 인간에서의 세포들에서 복제하지 않게 함을 의미한다. 복제-결손 아데노바이러스 벡터는 백신의 안전성을 보장한다. 한 가지 구체예에서, 아데노바이러스 벡터는 E1 또는 E3 또는 이들 둘 모두를 제거한 벡터이다. 유전자에서의 결핍은 유전자의 기능을 완전히 제거하거나 손상시켜서, 예를 들어, 유전자 생성물의 기능이 천연 유전자에 비해서 적어도 약 2-배, 5-배, 10-배, 20-배 또는 그 초과까지 감소되게 하기 위한 돌연변이 또는 결실로서 정의된다. 생성되는 복제-결손 아데노바이러스 벡터는, 하나 이상의 요망되는 단백질의 발현을 위해서, 아데노바이러스 게놈 내의 적절한 부위에 하나 이상의 외인성 핵산 서열을 수용할 수 있으면서, 아데노바이러스 캡시드내로 포장되는 능력을 유지한다. 원액을 위한 바이러스 벡터의 높은 역가를 생성시키기 위한 목적으로, 복제-결손 아데노바이러스 벡터는 전형적으로는 복제-결손 아데노바이러스 벡터에 존재하지 않는 유전자 기능을 제공하는 보완 세포주, 예컨대, HEK293 또는 HEK293R 세포에서 생산된다.

상기 아데노바이러스는 아데노바이러스 혈청형 2(Adenovirus serotype 2, Ad2), 아데노바이러스 혈청형 4(Adenovirus serotype 4, Ad4), 아데노바이러스 혈청형 5(Adenovirus serotype 5, Ad5), 아데노바이러스 혈청형 11(Adenovirus serotype 11, Ad11), 아데노바이러스 혈청형 26(Adenovirus serotype 26, Ad26), 아데노바이러스 혈청형 35(Adenovirus serotype 35, Ad35), 침팬지 아데노바이러스 혈청형 68(Chimpanzee adenovirus serotype 68, ChAd68), 조류 아데노바이러스 혈청형 9(Fowl adenovirus serotype 9, FAd9) 또는 돼지 아데노바이러스 혈청형 3(Porcine Adenovirus serotype 3, PAd3) 일 수 있다.

상기 아데노바이러스는 Ad5를 기본으로 하여 변형된 형태일 수 있다.

상기 아데노바이러스는 아데노바이러스 혈청형 5(Adenovirus serotype 5, Ad5)의 knob 유전자가 아데노바이러스 혈청형 35(Adenovirus seroitype 35, Ad35)의 knob 유전자로 치환된 아데노바이러스(Ad5/35)일 수 있다.

상기 아데노바이러스는 E1 유전자 및 E3 유전자가 결실된 것을 특징으로 한다.

상기 아데노바이러스는 E1 유전자 및 E3 유전자 결실에 더하여 E4 유전자의 일부 또는 전부가 결실되고 E4 유전자가 E1 영역으로 재배치한 형태일 수 있다.

상기 아데노바이러스는 인간 면역 세포에서 높게 발현하는 CD46 수용체를 통하여 세포 내로 도입되는 것을 특징으로 한다.

상기 아데노바이러스는 간 독성 유발 효과가 낮은 것을 특징으로 한다.

상기 아데노바이러스는 항원 발현도가 높은 것을 특징으로 한다.

또한, 본 발명은 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자의 폴리뉴클레오티드를 포함하는 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

상기 코로나바이러스는 SARS-CoV, MERS-CoV 및 SARS-CoV-2로 이루어진 군으로부터 선택되는 어느 1종일 수 있다.

상기 벡터는 벡터로 사용될 수 있는 공지의 바이러스는 모두 사용할 수 있으며, 아데노바이러스, 레트로바이러스, 렌티바이러스, 아데노 연관 바이러스(Adeno-associated virus, AAV), modified vaccinia virus ankara(MVA)중 어느 1종 일 수 있으나, 이에 한정되는 것은 아니다. 본 발명의 구체적인 실시예에 따르면 상기 바이러스는 아데노바이러스인 것이 바람직하다.

상기 아데노바이러스는 아데노바이러스 혈청형 2(Adenovirus serotype 2, Ad2), 아데노바이러스 혈청형 4(Adenovirus serotype 4, Ad4), 아데노바이러스 혈청형 5(Adenovirus serotype 5, Ad5), 아데노바이러스 혈청형 11(Adenovirus serotype 11, Ad11), 아데노바이러스 혈청형 26(Adenovirus serotype 26, Ad26), 아데노바이러스 혈청형 35(Adenovirus serotype 35, Ad35), 침팬지 아데노바이러스 혈청형 68(Chimpanzee adenovirus serotype 68, ChAd68), 조류 아데노바이러스 혈청형 9(Fowl adenovirus serotype 9, FAd9) 또는 돼지 아데노바이러스 혈청형 3(Porcine Adenovirus serotype 3, PAd3)일 수 있다.

상기 아데노바이러스는 Ad5를 기본으로 하여 변형된 형태일 수 있다.

상기 아데노바이러스는 아데노바이러스 혈청형 5(Adenovirus serotype 5, Ad5)의 knob 유전자가 아데노바이러스 혈청형 35(Adenovirus seroitype 35, Ad35)의 knob 유전자로 치환된 아데노바이러스(Ad5/35)일 수 있다.

상기 아데노바이러스는 E1 유전자 및 E3 유전자가 결실된 것을 특징으로 한다.

상기 아데노바이러스는 E1 유전자 및 E3 유전자 결실에 더하여 E4 유전자의 일부 또는 전부가 결실되고 E4 유전자가 E1 영역으로 재배치한 형태일 수 있다.

상기 아데노바이러스는 인간 면역 세포에서 높게 발현하는 CD46 수용체를 통하여 세포 내로 도입되는 것을 특징으로 한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질의 S1 및 S2 유전자의 폴리뉴클레오티드를 포함하는 벡터를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자의 폴리뉴클레오티드를 아데노바이러스에 도입한 것을 특징으로 하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명은 SARS-CoV-2 스파이크 단백질(Spike protein)의 S1 및 S2 유전자의 폴리뉴클레오티드를 Ad5/35 바이러스에 도입한 것을 특징으로 하는 코로나바이러스 감염증 예방 또는 치료용 백신을 제공한다.

또한, 본 발명의 백신은 경구형 또는 비경구형 제제로 제조할 수 있으며, 진피내, 근육내, 비강 경로로 투여할 수 있고, 바람직하게는 근육 내 경로로 투여될 수 있다.

본 발명의 백신은 상기 S1 및 S2 단백질 사이의 절단 인지 서열(cleavage site)이 제거된 것을 특징으로 한다.

상기 백신은 스파이크 단백질의 S1 및 S2 사이의 절단 인지 서열이 제거되고 링커 서열로 연결되는 것을 특징으로 한다.

상기 링커는 9 내지 45개의 염기로 구성되는 것을 특징으로 하고, 바람직하게는 9 내지 21개의 염기로 구성되며, 더 바람직하게는 15개의 염기로 구성된다.

또한, 본 발명은 상기 백신을 개체에 투여하여 코로나바이러스감염증-19, 중증급성호흡기증후군(SARS 또는 중동호흡기증후군(MERS)를 포함하는 코로나바이러스 감염증을 예방 또는 치료하는 방법을 제공한다.

본 발명에 있어서, "개체"란 코로나바이러스에 감염될 수 있는 인간을 포함한 모든 동물을 의미한다. 본 발명의 백신을 개체에 투여함으로써, 상기 질환을 효율적으로 예방할 수 있다. 예를 들어, 본 발명의 백신으로 인간을 코로나바이러스로부터 예방할 수 있다.

본 발명에 있어서, "예방"이란 백신의 투여에 의해 코로나바이러스 감염을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.

본 발명에 있어서, "치료"란 백신의 투여에 의해 코로나바이러스를 제거하거나 코로나바이러스로 인한 증상을 경감시키는 모든 행위를 의미한다.

본 발명의 백신은 약제학적으로 유효한 양으로 투여한다. 용어 "약제학적으로 유효한 양"은 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분한 양을 의미하며, 유효 용량 수준은 개체의 종류 및 중증도, 연령, 성별, 감염된 바이러스 종류, 약물의 활성, 약물에 대한 민감도, 투여 시간, 투여 경로, 배출 비율, 치료 기간, 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다. 본 발명의 백신은 개별 치료제로 투여하거나 다른 치료제와 병용하여 투여될 수 있고 종래의 치료제와는 순차적 또는 동시에 투여될 수 있다. 그리고 단일 또는 다중 투여될 수 있다. 상기 요소를 모두 고려하여 부작용 없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하며, 당업자에 의해 용이하게 결정될 수 있다.

용어 "유효한 양"은 치료되는 대상자에게 요망되는 치료 효과를 제공하기에 충분한, 예를 들어, 이의 수용체에서의 병원체 또는 항원에 대항하는 면역 반응을 생성시키거나 유도하기에 충분한 용량을 나타낸다. 유효량은 다양한 이유, 예컨대, 투여 경로 및 빈도, 상기 약제를 수용하는 개개의 체중 및 종, 및 투여 목적에 따라서 변화될 수 있다. 당업자는 본원에서의 개시내용, 확립된 방법 및 그들 자신의 경험을 기초로 하여 각각의 경우에서의 투여량을 결정할 수 있다. 예를 들어, 특정의 구체예에서, 본 발명의 재조합 아데노바이러스 벡터는 1×10⁹ 내지 1×10¹² 바이러스 입자(VP), 예를 들어, 1×10⁹, 1×10¹⁰, 1×10¹¹, 또는 1×10¹² VP의 용량으로 투여될 수 있다.

본 발명의 구체적인 실시예에서, 본 발명자들은 코로나바이러스에 속하는 SARS-CoV-2의 스파이크 단백질을 코딩하는 폴리뉴클레오티드의 S1 및 S2 사이의 절단 인지 서열을 링커 서열로 치환한 폴리뉴클레오티드를 아데노바이러스의 E1와 E3 유전자가 결실되어 있으며, 아데노바이러스 혈청형 5의 세포수용체 결합부위인 파이버(Fiber)를 혈청형 35의 파이버로 치환한 아데노바이러스에 도입한 벡터를 제조하고(도 4 참조), 상기 벡터가 항원 발현도, 항체 생성량 및 T 세포 반응성이 높고, 항체 생성 발현 기간이 길며, 간 독성을 보이지 않음을 확인하였다(도 6, 도 7, 도 9, 도 12, 도 13 및 도 14). 또한, Ad5/35 벡터 뿐만 아니라 E1 유전자 및 E3 유전자 결실에 더하여 E4 유전자의 일부 또는 전부가 결실되고 E4 유전자가 E1 영역으로 재배치된 벡터에 항원을 탑재하여 제조한 백신을 마우스 및 원숭이에 투여한 결과 중화항체 생성량이 Ad5/35 벡터에 항원이 탑재된 백신과 유사한 것을 확인하였다(도 15 및 도 16).

따라서, 본 발명의 코로나바이러스 재조합 스파이크 단백질은 안정하여 세포 내에서 잘 분해가 되지 않고, 면역세포를 효과적으로 활성화시켜 항체 생성량 및 T 세포 반응성이 높고, 본 발명의 벡터는 항원 발현도가 높게 나타남에 따라, 항체 생성량 및 T 세포 반응성이 높고, 항체 생성 발현 기간이 길었으며, 간 독성을 보이지 않음이 확인되었으므로, 코로나바이러스 감염증 예방 또는 치료용 백신으로 유용하게 사용될 수 있다.

본 발명을 하기 실시예 및 실험예에 의해 상세히 설명한다.

단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 의해 한정되는 것은 아니다.

< 실시예 1> 대조군 발현 벡터 제작 (플라스미드 #1 벡터)

스파이크 단백질 항원이 포함되지 않은 아데노바이러스 서열(서열번호 21)을 포함한 플라스미드를 pAdk35F(서열번호 1, 34,030 bp)로 명명하고 아데노바이러스 혈청형 5(Adenovirus type 5, Ad5)의 유전체를 사용하여 제작하였다. pAdk35F는 아데노바이러스의 E1와 E3 유전자가 결실되어 있으며, 아데노바이러스 혈청형 5의 세포수용체 결합부위인 파이버(Fiber)를 혈청형 35의 파이버로 치환한 형태의 아데노바이러스를 포함한다. pAdk35F의 E1 유전자 결실 부위에 CMV(Cytomegalovirus) 프로모터와 테트라싸이클린 오퍼레이터(Tetracycline Operator, Tet O), 제한효소 SwaI 부위, SV40 poly A 서열이 탑재되어 있다.

도 4에 나타난 바와 같이 항원 발현 유전자인 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 탑재한 pCMV3-SARS-CoV-2(Spike ORF) 벡터(Sino Biological, Cat. #: VG40589-UT, 서열번호 2, 9,858bp)를 주형으로 스파이크 단백질 항원 유전자의 세포 외 도메인 부위를 하기 표 1의 스파이크 단백질 항원 증폭용 프라이머 세트를 이용하여 증폭시킨 다음, 제한효소 SwaI으로 절단한 pAdk35F 벡터와 인퓨전 클로닝 라이게이션(in-fusion cloning ligation, Clontech, Cat. #: 639648)하여 대조군 발현 벡터인 플라스미드 #1 벡터(서열번호 22)를 제작하였다. 상기 플라스미드 #1 벡터에서 스파이크 단백질을 코딩하는 부분은 서열번호 3에, 스파이크 단백질이 도입된 아데노바이러스의 염기서열은 서열번호 23에 나타나 있다.

	서열(5'→3')	서열번호
정방향 프라이머	TCC AGC CTC CGA TTT GCC ACC ATG TTT GTG TTC CTG G	4
역방향 프라이머	GAT TAT GAT CAA TTT TCA TGG CCA CTT GAT GTA TTG TTC	5

< 실시예 2> 개량형 스파이크 항원 발현 벡터 제작

<2-1> 개량형 스파이크 항원 발현 벡터의 제작 (플라스미드 #2 벡터)

상기 실시예 1에서 중합효소 연쇄반응의 주형으로 사용한 pCMV3-SARS-CoV-2(Spike ORF) 벡터의 스파이크 단백질 항원의 도메인 S1과 S2의 절단 인지 서열(서열번호 6, 아미노산 서열: TNSPRRAR)을 부위 지향 돌연변이 유발 기법(Site Directed Mutagenesis)을 이용하여 하기 표 2의 프라이머 세트로 TILR(Thr-Ile-Arg-Leu) 서열(서열번호 7)로 치환하여 pCMV3-SARS-CoV-2(Spike IL) 도 5와 같이 벡터를 제작하였다.

이후 TILR 서열로 치환된 벡터를 주형으로 플라스미드 #1 벡터와 동일한 상기 표 1의 스파이크 항원 증폭용 프라이머 세트를 사용하여 증폭한 다음, 제한효소 SwaI으로 절단한 pAdk35F 벡터와 인퓨전 클로닝 라이게이션(in-fusion cloning ligation, Clontech, Cat. #: 639648)하여 개량형 스파이크 항원 발현 벡터인 플라스미드 #2 벡터(서열번호 24)를 제작하였다. 상기 플라스미드 #2 벡터에서 스파이크 단백질을 코딩하는 부분은 서열번호 8에, 스파이크 단백질이 도입된 아데노바이러스의 염기서열은 서열번호 25에 나타나 있다.

	서열(5'→3')	서열번호
정방향 프라이머	AGA CCA TCC TCA GGT CTG TGG CAA GCC AGA G	9
역방향 프라이머	CCT CCT ACC AGA CCC AGA CCA TCC TCA GGT	10

<2-2> 개량형 스파이크 항원 발현 벡터의 제작 (플라스미드 #3 벡터; pAdCLD-CoV19 벡터)

상기 실시예 1에서 중합효소 연쇄반응의 주형으로 사용한 pCMV3-SARS-CoV-2(Spike ORF) 벡터의 스파이크 항원의 도메인 S1과 S2의 절단 인지 서열(서열번호 6, 아미노산 서열: TNSPRRAR)을 부위지향 돌연변이 유발 기법(Site Directed Mutagenesis)을 이용하여 하기 표 3의 프라이머를 이용하여 TGGGGSR 링커 서열(서열번호 11)로 치환하여 pCMV3-SARS-CoV-2(Spike GGGGS) 벡터를 제작하였다. 이후 TGGGGSR 서열로 치환된 벡터를 주형으로 #1 벡터와 동일한 상기 표 1의 스파이크 항원 증폭용 프라이머 세트를 사용하여 증폭한 다음, 제한효소 SwaI으로 절단한 pAdk35F 벡터와 인퓨전 클로닝 라이게이션(in-fusion cloning ligation, Clontech, Cat. #: 639648)하여 개량형 스파이크 항원 발현 벡터인 플라스미드 #3 벡터(pAdCLD-CoV19 벡터)(서열번호 26)를 제작하였다. 상기 플라스미드 #3 벡터에서 스파이크 단백질을 코딩하는 부분은 서열번호 12에, 스파이크 단백질이 도입된 아데노바이러스의 염기서열은 서열번호 27에 나타나 있다.

	서열(5'→3')	서열번호
정방향 프라이머	CGG TGG CGG TGG GTC GAG GTC TGT GGC AAG CCA GAG	13
역방향 프라이머	GAC CCA CCG CCA CCG GTC TGG GTC TGG TAG GAG G	14

<2-3> 개량형 스파이크 항원 발현 벡터의 제작 (플라스미드 #4 벡터)

상기 실시예 1에서 중합효소 연쇄반응의 주형으로 사용한 pCMV3-SARS-CoV-2(Spike ORF) 벡터의 스파이크 항원의 도메인 S1과 S2의 절단 인지 서열 (아미노산 서열: TNSPRRAR)을 부위지향 돌연변이 유발 기법(Site Directed Mutagenesis)을 이용하여 하기 표 4의 프라이머를 이용하여 TGGGGSGGGGSGGGGSR 서열(서열번호 15)로 치환하였다(pCMV3-SARS-CoV-2(Spike GGGGSx3)). 이후 TGGGGSGGGGSGGGGSR 서열로 치환된 벡터를 주형으로 플라스미드 #1 벡터와 동일한 스파이크 항원 증폭용 프라이머 세트를 사용하여 증폭한 다음, 제한효소 SwaI으로 절단한 pAdk35F 벡터와 인퓨전 클로닝 라이게이션(in-fusion cloning ligation, Clontech, Cat. #: 639648)하여 개량형 스파이크 항원 발현 벡터인 플라스미드 #4 벡터(서열번호 28)를 제작했다. 상기 플라스미드 #4 벡터에서 스파이크 단백질을 코딩하는 부분은 서열번호 16에, 스파이크 단백질이 도입된 아데노바이러스의 염기서열은 서열번호 29에 나타나 있다.

	서열(5'→3')	서열번호
정방향 프라이머	GGG CGG TGG TGG GTC GGG TGG CGG CGG TTC CAG GTC TGT GGC AAG CCA GAG	17
역방향 프라이머	GAC CCA CCA CCG CCC GAC CCA CCG CCA CCG GTC TGG GTC TGG TAG GAG G	18

< 실시예 3> 스파이크 항원 탑재 아데노바이러스 벡터의 제작 (#1 내지 #4 벡터)

상기 실시예 1 및 2의 벡터(플라스미드 #1~#4 벡터)는 HEK293R 세포주를 이용하여 아데노바이러스 벡터(#1~#4 벡터)를 생산했다. 아데노바이러스는 T25 플레이트에 약 80% 차 있는 HEK293R 세포주에 25㎕의 리포펙타민 2000(ThermoFisher, Cat. #: 11668027)과 함께 각 12.5㎍의 벡터를 형질 전환 시켜 초기 아데노바이러스 벡터를 생산했다. 초기 생산된 각 아데노바이러스는 4×10⁷개의 HEK293R 세포주가 있는 T175 플라스크 20장에 감염시켜 증폭시켰다.

이후 증폭한 아데노바이러스 #1~#4 벡터는 세슘클로라이드(CsCl) 밀도 구배 원심분리법(cesium chloride density gradient centrifugation)을 1차(1.2g/㎖ CsCl+1.4 g/mL CsCl, 32,000 RPM, 90분)와 2차(1.35g/㎖ CsCl, 32,000 RPM, 18시간)에 걸쳐 정제하였고, 투석(20mM Tris-HCl, 25mM 염화나트륨, 2.5% 글리세롤)을 통해 완제 아데노바이러스를 생산하였다.

< 실시예 4> 시험관 내(in vitro)에서 항원 발현 벡터의 항원 발현도 비교

<4-1> 항원 발현 벡터 주입에 따른 세포 내 항원 발현도 비교

상기 실시예 3에서 제작한 #1 내지 #4 벡터의 세포 내 항원 발현도를 비교하기 위해 단핵 세포 유래의 THP-1 세포주에서의 항원 발현도를 유세포 분석법(FACS)으로 측정하였다.

구체적으로, 유세포 분석법은 96-웰 배양 접시에 THP-1 세포가 각 웰 당 2.5 × 10⁵ 세포가 되도록 분주한 후, 각 벡터를 10 MOI (Multiplicity of infection)가 되도록 세포에 접종한 다음, 24시간 동안 배양하였다. 이후 각 웰의 세포를 각각 1.5 ㎖ 튜브로 옮기고 FACS 버퍼 500㎕를 넣고 원심분리(5000rpm, 3분, 4℃) 시켰다. 상층액을 제거한 후, 1:1000으로 희석한 생존도 지시염료 (eFluor™450, ebioscience, cat #:65-0863-14) 를 각 50㎕씩 넣고 4℃에서 30분 동안 표면염색을 수행하였다.

세포 내부에서 발현하는 항원 검출을 위해, 세포 고정/투과 농축액(ebioscience, Cat. #: 00-5123-43)을 세포 고정/투과 희석액(ebioscience, Cat. #: 00-5223-56)에 1:4로 희석하여 각 100㎕씩 넣고 4℃에서 30분 동안 세포 고정/투과를 진행했다. 스파이크 항원 염색은 SARS-CoV-2 스파이크 단백질 항체(GeneTex, Cat. #: GTX632604)를 투과 버퍼(ebioscience, Cat. #: 00-8333-56)에 1:2500으로 희석한 용액(항체 농도: 0.4㎍/㎖)으로 4℃에서 1시간 동안 진행 후 워싱한 다음, 2차 항체인 APC Goat anti-mouse IgG antibody(Biolegend, Cat: 405308)를 1:100으로 희석한 용액(항체 농도: 2㎍/㎖)으로 항원 염색(4℃, 30분)했다. 염색 반응 후, 시료당 300㎕ 버퍼를 넣고 유세포 분석기(BD bioscience, LSRFORESSA)로 항원 발현 정도를 측정하였다.

그 결과, 도 6에 나타난 바와 같이, 실시예 3에서 제작한 아데노바이러스 #3 벡터가 단백질을 생성하는 능력인 항원 발현도가 가장 높은 것을 확인하였다.

<4-2> 항원 발현 벡터 주입에 따른 세포 밖 항원 발현도 비교

상기 실시예 3에서 제작한 #1 내지 #4 벡터의 세포 밖 항원 발현도를 비교하기 위해 단핵 세포 유래의 THP-1와 근육 세포 유래의 RD 세포주에서의 항원 발현도를 웨스턴 블랏 분석법으로 측정하였다.

구체적으로, 세포 밖으로 배출되는 스파이크 단백질 항원 발현도 측정을 위한 웨스턴 블랏 분석법은 다음과 같다. 6-웰 배양 접시에 THP-1 및 RD 세포가 각 웰 당 5 × 10⁵ 세포가 되도록 분주한 후, 각 벡터를 50 MOI가 되도록 세포에 접종한 다음 24시간 동안 배양하였다. 이후 원심분리를 통해 배양 배지를 수확한 다음 Microcon (Millipore, 50,000 MWCO, Cat. #: UFC805024)를 이용하여 배양 배지를 농축시켰으며, 배양한 세포는 RIPA 버퍼(ThermoFisher, Cat. #: 89901)를 이용하여 단백질 단위로 분해시켰다. 농축한 배양 배지의 단백질 총량은 BCA 단백질 어세이 키트(Thermo Fisher, Cat. #: 23225)를 이용하여 측정하였다.

농축된 배양 배지 시료에 스파이크 단백질(삼량체, 약 250 kDa 이상)을 확인하기 위해 BoltTM Bis-Tris 4-12% plus, 젤(ThermoFisher, Cat. #: NW04120BOX)을 이용하여 60㎍/웰의 시료를 180V에서 전기 영동하였다.

전기영동한 젤은 iBlot 2 Dry blotting system (Invitrogen, Cat. #: IB21001)을 이용하여 20V에서 10분간 PVDF 막에 단백질을 이동시켰다. 이후 Rabbit SARS-CoV-2 spike 항체(GeneTex, Cat. #: GTX135360)과 Goat anti-Rabbit IgG H&L (HRP)에 각각 반응시킨 후 ECL prime western blotting detection reagent (Amersham, Cat. #: RPN2232)로 SARS-CoV-2가 가지고 있는 삼량체 스파이크 단백질을 검출하였다.

그 결과, 도 7에 나타난 바와 같이, 실시예 3에서 제작한 아데노바이러스 #3 벡터가 단백질을 생성하고 배출하는 항원 발현도가 가장 높은 것을 확인하였다.

< 실시예 5> 항원 발현 벡터 주입에 따른 마우스에서 항체 생성량의 비교

<5-1> #1 내지 #4 항원 발현 벡터 주입에 따른 마우스 항체 생성량의 비교

상기 실시예 3에서 제작한 #1 내지 #4 벡터를 마우스에 주입 후 항체 생성량을 비교하였다.

구체적으로, 상기 실시예 3에서 제작한 스파이크 항원 발현 벡터(#1 벡터~ #4 벡터)를 6~7주령 BALB/c 마우스에 각 그룹당 6마리씩 2×10⁸ IFU/마우스로 근육주사를 수행했다. 투여 후 마우스에서 2-3주차 시기에 안와 채혈로 약 300㎕의 혈액 시료를 채취했다. 이후 원심분리(8000rpm, 10분, 20℃)를 통해 혈장을 분리하여, 혈액 내에 존재하는 중화 항체의 양을 효소결합면역흡착검사(ELISA)를 통해 측정했다.

효소결합면역흡착검사는 96-웰 플레이트에 스파이크 단백질(Acro Biosystems, Cat. #: SPN-C52H84)을 PBSN(PBS 1 L + Sodium Azide 0.01 g)에 녹여 100 ng/웰로 코팅해준 다음, 16시간 동안 4℃에서 냉장 반응시켰다. 96-웰 플레이트 코팅 16시간 후 코팅 단백질은 제거하고 PBS로 3회 세척한 뒤, 150㎕ 차단 버퍼(Blocking buffer, PBSN+BSA 1%)를 각 웰에 넣고 반응(37℃, 90분)시켰다. 반응시간 동안 희석 버퍼(Dilution buffer. PBSN+0.1% BSA+0.05% Tween-20)에 혈장을 6400배 희석하고, 차단 반응이 끝난 후 플레이트를 PBS로 3회 세척 후 희석한 혈장 샘플을 50㎕씩 웰에 넣고 반응(37℃, 3시간)시켰다. 반응 이후, PBS 3회 세척 및 2차 항체인 GAM-IgG-HRP(southernbiotech, Cat. #: 1030-05), GAM-IgM-HRP(southernbiotech, Cat. #: 1020-05)을 희석 버퍼에 1000배 희석하여 50㎕씩 웰에 넣고 반응(37℃, 2시간)하였다. 이후 2차 항체를 제거하고 PBS 5회 세척 후, 발색 시약(TMB Peroxidase Substrate buffer, ROCKLAND, Cat. #: TMBE-1000)을 50 ㎕씩 넣고 15분간 발색시키고 0.25N HCl을 50㎕ 넣어 발색을 멈추고, 시료를 마이크로리더기로 450nm 파장에서 분석하였다.

그 결과, 도 8에 나타난 바와 같이, 실시예 3에서 제작한 아데노바이러스 #3 벡터의 항체 생성량이 가장 높은 것으로 나타났다.

<5-2> 항원 발현 벡터 주입에 따른 마우스 항체 생성 발현 기간 비교

또한, 상기 실시예 3에서 제작한 아데노바이러스(#1 내지 #3 벡터)는 8주령 BALB/c 마우스에 각 그룹당 6마리씩 1×10⁹ VP/Mouse 또는 2×10⁸ VP/Mouse로 근육주사를 수행했다. 투여 후 마우스에서 2 내지 4주차 시기에 안와 채혈을 통해 약 300㎕의 혈액 시료를 채취했다. 이후 원심분리(8000rpm, 10분, 20℃)를 통해 혈장을 분리하여, 혈액 내에 존재하는 항원 특이 항체의 양을 실시예 5-1에 따라 효소결합면역흡착검사(ELISA)를 통해 측정하였다.

그 결과, 도 9에 나타난 바와 같이 실시예 3에서 제작한 아데노바이러스 #3 벡터의 혈액 내에 존재하는 항원 특이 항체의 양이 #2 벡터보다 4주 이상 높은 수준을 유지하는 것으로 나타났다.

< 실시예 6> 항원 발현 벡터 주입에 따른 원숭이에서 항체 생성량 및 간독성 의 비교

<6-1> 항원 발현 벡터 주입에 따른 원숭이에서 항체 생성량의 비교

상기 실시예 3에서 제작한 아데노바이러스(#1 및 #3 벡터) 주입에 따른 원숭이에서 항체 생성량을 비교하였다.

구체적으로, 상기 실시예 3에서 제작한 아데노바이러스 #1 및 #3 벡터를 6~7주령 원숭이(cynomolgus monkey)에 각 그룹당 2마리씩 1×10¹¹ VP/Monkey로 근육주사를 수행했다. 면역화 후 2, 3주차에 원숭이의 정맥채혈을 통해 혈액시료를 채취하였으며, 혈구를 분리(8000rpm, 10분, 20℃)한 혈장을 사용하여 혈액 내에 존재하는 항체의 양을 효소결합면역흡착검사(ELISA) 및 슈도바이러스 중화 분석(Pseudovirus Neutralization Assay)를 통해 측정하였다.

효소결합면역흡착검사는 96-웰 플레이트에 스파이크 단백질(Acro Biosystems, Cat. #: SPN-C52H84)을 PBSN에 녹여 100 ng/웰로 코팅해준 다음, 16시간동안 4℃에서 냉장 반응시켰다. 96-웰 플레이트 코팅 16시간 후 코팅 단백질은 제거하고 PBS로 3회 세척한 뒤, 150㎕의 차단 버퍼를 각 웰에 넣고 반응(37℃, 90분)했다. 반응시간 동안 희석 버퍼에 혈장을 1600배 희석하고, 차단 반응이 끝난 후 플레이트를 PBS로 3회 세척 후 희석한 혈장 샘플을 50㎕씩 웰에 넣고 반응(37℃, 3시간)시켰다. 반응 이후, PBS 3회 세척 및 2차 항체인 Human total Ig-HRP(southernbiotech, Cat. #: 1010-05)을 희석 버퍼에 1000배 희석하여 50㎕씩 웰에 넣고 반응(37℃, 2시간)하였다. 이후 2차 항체를 제거하고 PBS 5회 세척 후, 발색 시약(TMB Peroxidase Substrate buffer, ROCKLAND, Cat. #: TMBE-1000)을 50㎕ 씩 넣고 15분간 발색시키고 0.25N HCl을 50㎕ 넣어 발색을 멈춘 후 시료를 마이크로리더기의 450nm 파장에서 분석하였다.

슈도바이러스 중화 분석은 1×10⁴ 세포/웰의 HEK293T-hACE2(인간 안지오텐신 전환효소 2; hACE2) 세포주를 96-웰 플레이트의 각 웰에 넣고 10시간 배양(37℃, 5% CO₂) 했다. 면역화 후 2, 3주차에 원숭이의 정맥채혈을 통해 채취한 혈장은 100~6400배 4배수 희석하여 7×10⁵ TU/㎖의 슈도바이러스(스파이크 단백질과 루시퍼레이즈를 발현하는 렌티바이러스)와 함께 1시간 반응(37℃)시킨 후, 배양 중인 HEK293T-hACE2 96-웰 플레이트에 2㎍/웰(200㎕) 폴리브렌(Merck, Cat. #: TR-1003-G)과 함께 접종하고 2일간 배양했다. 이후 스파이크 단백질에 결합하는 혈장 내 중화 항체 존재 여부는 슈도바이러스가 HEK293T-hACE2 세포주에 감염되어 루시퍼레이즈 단백질을 발현하는 정도를 측정하여 확인하였다. 2일간 배양한 세포는 DPBS 워싱 후 25㎍ 세포 용해 시약(Promega, Cat. #: E153A)으로 용해시켰다. 용해된 시료는 불투명 96-웰 플레이트에 옮긴 후 100㎍ 루시퍼레이즈 검출 지시약 (Promega, Cat. #: E151A)을 넣고 루미노미터(Luminometer Centro XS3 LB960, Berthold Technologies, Cat. #: LB960, software: Mikro 2000 program)를 이용하여 발광 정도를 측정(검출 지시약 주입-5초 섞임-2초 지연-10초 측정)하여 분석하였다.

그 결과, 도 10에 나타난 바와 같이, #3 벡터를 주입한 경우 항체 생성량이 우수하였고, 벡터 주입 후 5주가 지나도 항체 역가가 유지되는 것을 확인하였다. 또한, 도 11에 나타난 바와 같이, #3 벡터를 주입한 경우 중화 항체 농도가 높은 것을 확인하였다. 상기 결과는 백신 투여 2주 후부터 다량의 중화 항체가 생성되기 시작하여 바이러스의 세포 감염을 효과적으로 차단할 수 있음을 제시한다.

<6-2> 항원 발현 벡터 주입에 따른 원숭이에서 간 독성 비교

아데노바이러스 특성상 대부분 간으로 이동하여 간 독성을 나타낼 우려가 있고, 간 독성을 나타낼 경우 인체에 치명적이므로 고용량의 바이러스 입자를 투여해도 간 독성을 나타내지 않는 백신을 제조하는 것이 매우 중요하다. 따라서 상기 실시예 3에서 제작한 아데노바이러스(#1 및 #3 벡터) 주입에 따른 원숭이에서 간 독성을 비교하였다.

상기 실시예 3에서 제작한 아데노바이러스 #1 및 #3 벡터를 6~7주령 원숭이(cynomolgus monkey)에 각 그룹당 2마리씩 1×10¹¹ VP/Monkey로 근육주사를 수행했다. 모든 동물에서 투여 전(pre), 9, 22, 36일 간격으로 혈액을 채취하였다. 모든 채혈은 절식(음수는 자유섭취) 상태에서 실시하며, 일회용 주사기(3㎖, 23G, 한국백신, KOR)를 이용하여 대퇴정맥(Femoral vein)에서 약 3㎖을 채혈하였다.

혈액생화학적 검사를 위해 채혈한 혈액에서 약 0.6 ㎖을 SSTTM tube(Vacutainer®, BD, USA)에 분주하고 13,000 rpm에서 5분 동안 원심분리(5424R, Eppendorf, USA)하여 혈청을 분리한 다음, 생화학분석기(7180, HITACHI, JPN)를 이용하여 간 독성 수치인 아스파테이트아미노전이효소(Aspartate Aminotransferase, AST), 알라닌아미노전이효소(Alanine Aminotransferase, ALT) 항목을 측정하였다.

그 결과, 도 12에 나타난 바와 같이, 동일한 아데노바이러스 벡터 플랫폼을 사용한 #1 및 #3 벡터의 원숭이에서 고용량의 바이러스 입자 투여에도 불구하고 심각한 간 독성을 보이지 않았음을 확인하였다.

< 실시예 7> 항원 발현 벡터를 마우스 및 원숭이에 주입하였을 때의 T 세포 반응성 측정

<7-1> 마우스의 비장 세포 및 말초 혈액 단핵 세포( PBMC )에서 T 세포 반응성 측정

상기 실시예 3에서 제작한 스파이크 항원 발현 벡터(#1 벡터, #3 벡터) 주입에 따른 마우스에서 T 세포 반응성을 하기와 같이 측정하였다.

구체적으로, 상기 실시예 5의 백신 (#1, #3 벡터) 투여 마우스를 투여 10주차에 각 1마리씩 희생하여 혈액을 안와 채혈을 통해 채취하고 비장을 분리했다. 비장세포는 70㎛ cell strainer (BD Bioscience, Cat. #: 352350)을 이용하여 분쇄하여 단일 세포 상태로 만든 후, ACK lysis buffer (Gibco, Cat. #: A1049201)를 이용하여 적혈구를 제거했다. 혈액 샘플은 Histopaque®-1077 (Sigma-Aldrich, Cat. #: 10771)을 이용한 밀도 구배 원심분리법을 이용하여 말초 혈액 단핵 세포(PBMC)를 분리했다. 분리한 비장 세포 및 PBMC는 세포 수를 측정한 후 2×10⁶ cells/㎖로 RF10 배양 배지(RPMI1640 + 10% FBS + 1% penicillin/streptomycin)에 현탁 시켰다.

마우스 IFN-γ ELISPOT kit (CTL, Cat. #: MIFNgp-2M/10)에 코팅된 플레이트를 예열된 RF10 배양배지에 Peptivator SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat. #: 130-126-701)을 50㎍/㎖의 농도로 첨가하여 100㎕씩 웰을 채워 20분간 37℃ CO₂ 배양기에 보관했다 (각 그룹당 웰은 duplicate로 진행했다). 그 후, 재현탁되어 있는 마우스 비장 세포와 PBMC 함유 배양액을 100㎕씩 웰에 첨가하고 37℃, CO₂ 배양기에서 배양했다. 양성 대조군 웰에는 항-CD3 mAb(1㎍/㎖, Biolegend, Cat. #: 100331)을 첨가하였고, 음성 대조군 웰에는 세포를 넣어주지 않고 RF10만 넣었다. 24시간 동안 배양 후, Mouse IFN-γ ELISPOT kit에 포함되어 있는 제조사의 프로토콜과 같은 방식으로 ELISPOT development를 진행했다. 발색 후 차광하여 24시간 동안 상온에서 플레이트를 건조시킨 후 Immunospot S6 micro analyzer (CTL)를 이용하여 분석했다. 결과는 1×10⁶ PBMC 단위로 환산하였다.

그 결과, 도 13에 나타난 바와 같이, #3 벡터를 마우스에 주입한 경우 마우스의 비장 세포 및 말초 혈액 단핵 세포(PBMC)에서 벡터를 주입하지 않은 군 및 #1 벡터를 주입한 군에 비해 T 세포 반응성이 유의하게 증가한 것을 확인할 수 있었다. 상기 결과는 본 발명의 백신 조성물은 SARS-CoV-2에 재감염되었을 때, 항체 생성뿐만 아니라 T 세포에 의한 기억 면역 반응도 활발하게 유도할 수 있음을 제시한다.

<7-2> 원숭이 말초혈액단핵세포(PBMC)에서 T 세포 반응성 측정

상기 실시예 3에서 제작한 아데노바이러스(#1 벡터, #3 벡터) 주입에 따른 원숭이에서 T 세포 반응성을 하기와 같이 측정하였다.

상기 실시예 6의 백신 (#3 벡터) 투여 원숭이를 투여 4주차에 정맥채혈을 통해 혈액샘플을 5㎖ 채취했다. 혈액 샘플은 Histopaque®-1077 (Sigma-Aldrich, Cat. #: 10771)을 이용한 밀도 구배 원심분리법을 이용하여 PBMC를 분리했고, 잔존하는 적혈구는 ACK lysis buffer (Gibco, Cat. #: A1049201)를 이용하여 제거했다. 분리한 PBMC는 세포수를 측정한 후 2E+06 cells/㎖로 RF10 media (RPMI1640 + 10% FBS + 1% penicillin/streptomycin)에 현탁했다.

Human IFN-γ ELISPOT kit (CTL, Cat. #: HIFNgp-2M/10)에 코팅된 플레이트를 예열된 RF10 배양 배지에 Peptivator SARS-CoV-2 Prot_S(Miltenyi Biotec, Cat. #: 130-126-701)을 100㎍/㎖의 농도로 첨가하여 100㎕씩 웰을 채워 20분간 37℃ CO₂ 배양기에 보관했다 (각 그룹당 웰은 2배수로 진행하였다). 그 후, 현탁된 PBMC 함유 배양배지를 100㎕씩 웰에 첨가하고 37℃, CO₂ 배양기에서 배양했다. (양성 대조군 웰에는 PMA(10ng/㎖)와 이오노마이신(1㎍/㎖)을 첨가하였고 음성 대조군 웰에는 세포를 넣어주지 않고 RF10만 넣었다. 24시간 동안 배양 후, Human IFN-γ ELISPOT kit에 포함되어 있는 제조사의 프로토콜과 같은 방식으로 ELISPOT development를 진행했다. 발색 후 차광하여 24시간 동안 상온에서 플레이트를 보관했다. 플레이트 건조가 끝나면 Immunospot S6 micro analyzer (CTL)를 이용하여 분석하여 결과를 도출하고 1× 10⁶ PBMC 단위로 환산하였다.

그 결과, 도 14에 나타난 바와 같이, #3 벡터를 원숭이에 주입한 경우 말초 혈액 단핵 세포(PBMC)에서 벡터를 주입하지 않은 군에 비해 T 세포 반응성이 유의하게 증가한 것을 확인할 수 있었다. 상기 결과는 본 발명의 백신 조성물은 SARS-CoV-2에 재감염되었을 때, 항체 생성뿐만 아니라 T 세포에 의한 기억 면역 반응도 활발하게 유도할 수 있음을 제시한다.

<실시예 8> #3 항원 발현 벡터 내지 #3 항원 이형 벡터 주입에 따른 마우스 및 원숭이에서의 항원 특이적 중화 항체 생성량 동등성 비교

<8-1> #3 항원 발현 벡터 내지 #3 항원 이형 벡터 주입에 따른 마우스에서의 항원 특이적 중화 항체 생성량 동등성 비교

상기 실시예 3에서 제작한 #3 벡터와, 동등성 비교군으로 제작한 #3 이형 벡터(서열번호 30)를 마우스에 주입 후 항체 생성량을 비교했다. #3 이형 벡터는 실시예 3에서 사용한 #3 벡터의 아데노바이러스 유전자 중 E4 유전자를 바이러스 유전체의 E1 영역으로 재배치한 형태이며, E1/E3 결손된 Ad5에서 E3의 knob 유전자가 Ad35로 치환된 Ad5/35 벡터에서, E4orf6의 711bp가 결실되고, 재조합 스파이크 단백질(SARS-CoV-2 S1 도메인 및 S2 도메인이 GGGGS 링커로 연결된 융합 단백질) 및 E4orf6 유전자가 정방향으로 E1 결실부위에 삽입된 형태로, 제작 방법은 실시예 3의 방식과 동일하다.

구체적으로, 스파이크 항원 발현 벡터(#3 벡터 및 #3 이형 벡터)를 6~7주령 BALB/c 마우스에 각 그룹당 5마리씩 1×10⁹ VP/마우스로 근육주사를 수행했다. 투여 후 마우스에서 9주차 시기에 안와 채혈로 약 300㎕의 혈액 시료를 채취했다. 이후 원심분리(8000rpm, 10분, 20℃)를 통해 혈장을 분리하여, 혈액 내에 존재하는 중화 항체의 양을 슈도바이러스 중화 분석(Pseudovirus Neutralization Assay)를 통해 측정했다.

슈도바이러스 중화 분석은 1×10⁴ 세포/웰의 HEK293T-hACE2(인간 안지오텐신 전환효소 2; hACE2) 세포주를 96-웰 플레이트의 각 웰에 넣고 10시간 배양(37℃, 5% CO₂) 했다. 면역화 후 8주차에 마우스의 정맥채혈을 통해 채취한 혈장은 100~6400배 4배수 희석하여 7×10⁵ TU/㎖의 슈도바이러스(스파이크 단백질과 루시퍼레이즈를 발현하는 렌티바이러스)와 함께 1시간 반응(37℃)시킨 후, 배양 중인 HEK293T-hACE2 96-웰 플레이트에 3일간 배양했다. 이후 스파이크 단백질에 결합하는 혈장 내 중화 항체 존재 여부는 슈도바이러스가 HEK293T-hACE2 세포주에 감염되어 루시퍼레이즈 단백질을 발현하는 정도를 측정하여 확인하였다. 3일간 배양한 세포는 DPBS 워싱 후 25㎍ 세포 용해 시약(Promega, Cat. #: E153A)으로 용해시켰다. 용해된 시료는 불투명 96-웰 플레이트에 옮긴 후 100㎍ 루시퍼레이즈 검출 지시약 (Promega, Cat. #: E151A)을 넣고 루미노미터(Luminometer Centro XS3 LB960, Berthold Technologies, Cat. #: LB960, software: Mikro 2000 program)를 이용하여 발광 정도를 측정(검출 지시약 주입-5초 섞임-2초 지연-10초 측정)하여 분석하였다.

그 결과, 도 15에 나타난 바와 같이, #3 항원 벡터와 #3 항원 이형 벡터는 우수한 항체 생셩량을 보였고, 두 그룹 간의 차이는 없었다. 상기 결과는 서로 다른 플랫폼 벡터에 탑재하더라도 #3 항원은 동등한 수준의 중화 항체 활성이 있음을 시사한다.

<8-2> #3 항원 발현 벡터 내지 #3 항원 이형 벡터 주입에 따른 원숭이에서의 항원 특이적 중화 항체 생성량 동등성 비교

상기 실시예 8-1의 #3 벡터 내지 동등성 비교군으로 제작한 #3 이형 벡터를 원숭이(cynomolgus monkey)에 주입 후 항원 특이적 중화 항체 생성량을 비교했다.

구체적으로, 스파이크 항원 발현 벡터(#3 벡터 및 #3 이형 벡터)를 6~7주령 원숭이에 각 그룹당 2마리씩 2×10¹⁰ VP/Monkey로 근육주사를 수행했다. 면역화 후 9주차에 원숭이의 정맥채혈을 통해 혈액시료를 채취하였으며, 혈구를 분리(8000rpm, 10분, 20℃)한 혈장을 사용하여 혈액 내에 존재하는 항체의 양을 슈도바이러스 중화 분석(Pseudovirus Neutralization Assay)를 통해 측정하였다.

슈도바이러스 중화 분석은 1×10⁴ 세포/웰의 HEK293T-hACE2(인간 안지오텐신 전환효소 2; hACE2) 세포주를 96-웰 플레이트의 각 웰에 넣고 10시간 배양(37℃, 5% CO₂) 했다. 면역화 후 9주차에 원숭이의 정맥채혈을 통해 채취한 혈장은 100~6400배 4배수 희석하여 7×10⁵ TU/㎖의 슈도바이러스(스파이크 단백질과 루시퍼레이즈를 발현하는 렌티바이러스)와 함께 1시간 반응(37℃)시킨 후, 배양 중인 HEK293T-hACE2 96-웰 플레이트에 3일간 배양했다. 이후 스파이크 단백질에 결합하는 혈장 내 중화 항체 존재 여부는 슈도바이러스가 HEK293T-hACE2 세포주에 감염되어 루시퍼레이즈 단백질을 발현하는 정도를 측정하여 확인하였다. 3일간 배양한 세포는 DPBS 워싱 후 25㎍ 세포 용해 시약(Promega, Cat. #: E153A)으로 용해시켰다. 용해된 시료는 불투명 96-웰 플레이트에 옮긴 후 100㎍ 루시퍼레이즈 검출 지시약 (Promega, Cat. #: E151A)을 넣고 루미노미터(Luminometer Centro XS3 LB960, Berthold Technologies, Cat. #: LB960, software: Mikro 2000 program)를 이용하여 발광 정도를 측정(검출 지시약 주입-5초 섞임-2초 지연-10초 측정)하여 분석하였다.

그 결과, 도 16에 나타난 바와 같이, #3 벡터 및 #3 이형 벡터를 주입한 경우 두 그룹 모두 항체 생성량이 우수하였고, 발현량에 차이를 보이지 않았다.

<실시예 9> 재조합 스파이크 단백질 주입에 따른 마우스에서 항체 생성량의 비교

<9-1> 재조합 스파이크 단백질 주입에 따른 마우스 항체 생성량의 비교

상기 실시예 3에서 제작한 #1 내지 #3 벡터로부터 유래한 재조합 스파이크 단백질(#2 재조합 단백질 및 #3 재조합 단백질)을 마우스에 주입 후 항체 생성량을 비교하였다.

구체적으로, 6~7주령 BALB/c 마우스에 각 그룹당 6마리씩 #1 내지 #3 벡터에서 유래한 재조합 스파이크 단백질을 주입하였다. #1 내지 #3 재조합 스파이크 단백질 10μg을 각 150㎕의 PBS용액(pH 7.4)에 희석하여 근육주사로 투여하였다. 투여 후 마우스에서 2 내지 4주차 시기에 안와 채혈로 약 300㎕의 혈액 시료를 채취하고, 원심분리(8000rpm, 10분, 20℃)를 통해 혈장을 분리하여, 혈액 내에 존재하는 중화 항체의 양을 효소결합면역흡착검사(ELISA) 및 슈도바이러스 중화 분석(Pseudovirus Neutralization Assay)를 통해 측정하였다.

효소결합면역흡착검사는 96-웰 플레이트에 스파이크 단백질(Acro Biosystems, Cat. #: SPN-C52H84)을 PBSN(PBS 1L + Sodium Azide 0.01g)에 녹여 100 ng/웰로 코팅해준 다음, 16시간 동안 4℃에서 냉장 반응시켰다. 96-웰 플레이트 코팅 16시간 후 코팅 단백질은 제거하고 PBS로 3회 세척한 뒤, 150㎕ 차단 버퍼(Blocking buffer, PBSN+BSA 1%)를 각 웰에 넣고 반응(37℃, 90분)시키고, 반응시간 동안 희석 버퍼(Dilution buffer. PBSN+0.1% BSA+0.05% Tween-20)에 혈장을 6400배 희석하고, 차단 반응이 끝난 후 플레이트를 PBS로 3회 세척 후 희석한 혈장 샘플을 50㎕씩 웰에 넣고 반응(37℃, 3시간)시켰다. 반응 이후, PBS 3회 세척 및 2차 항체인 GAM-IgG-HRP(southernbiotech, Cat. #: 1030-05), GAM-IgM-HRP(southernbiotech, Cat. #: 1020-05)을 희석 버퍼에 1000배 희석하여 50㎕씩 웰에 넣고 반응(37℃, 2시간) 시켰다. 이후 2차 항체를 제거하고 PBS 5회 세척 후, 발색 시약(TMB Peroxidase Substrate buffer, ROCKLAND, Cat. #: TMBE-1000)을 50 ㎕씩 넣고 15분간 발색 시키고 0.25N HCl을 50㎕ 넣어 발색을 멈추고, 시료를 마이크로리더기로 450nm 파장에서 분석하였다.

슈도바이러스 중화 분석은 1×10⁴ 세포/웰의 HEK293T-hACE2(인간 안지오텐신 전환효소 2; hACE2) 세포주를 96-웰 플레이트의 각 웰에 넣고 10시간 배양(37℃, 5% CO₂) 하였다. 면역화 후 2 내지 4주차에 마우스의 정맥채혈을 통해 채취한 혈장은 100~6400배(4 배수) 희석하여 7×10⁵ TU/㎖의 슈도바이러스(스파이크 단백질과 루시퍼레이즈를 발현하는 렌티바이러스)와 함께 1시간 반응(37℃)시킨 후, 배양 중인 HEK293T-hACE2 96-웰 플레이트에 3일간 배양하였다. 이후 스파이크 단백질에 결합하는 혈장 내 중화 항체 존재 여부는 슈도바이러스가 HEK293T-hACE2 세포주에 감염되어 루시퍼레이즈 단백질을 발현하는 정도를 측정하여 확인하였다. 3일간 배양한 세포는 DPBS 워싱 후 25㎍ 세포 용해 시약(Promega, Cat. #: E53A)으로 용해시킨 후 용해된 시료는 불투명 96-웰 플레이트에 옮긴 후 100㎍ 루시퍼레이즈 검출 지시약 (Promega, Cat. #: E151A)을 넣고 루미노미터(Luminometer Centro XS3 LB960, Berthold Technologies, Cat. #: LB960, software: Mikro 2000 program)를 이용하여 발광 정도를 측정(검출 지시약 주입-5초 섞임-2초 지연-10초 측정)하여 분석하였다.

<실시예 10> 스파이크 항원 플라스미드 주입에 따른 마우스에서 항체 생성량의 비교

<10-1> 스파이크 항원 플라스미드 주입에 따른 마우스 항체 생성량의 비교

상기 실시예 1, 2에서 제작한 #1 내지 #3 벡터를 플라스미드 형태(플라스미드 #1 벡터 및 플라스미드 #3 벡터)로 마우스에 주입 후 항체 생성량을 비교하였다.

구체적으로, 상기 실시예 1, 2에서 제작한 플라스미드 #2 내지 플라스미드 #3 벡터를 대장균에 도입하고 200 mL LB 배양배지에서 하루동안 배양하였다. 배양한 플라스미드는 QIAGEN Endofree Plasmid Maxi kit(Cat.# 12362)를 이용하여 수확한 후 6~7주령 BALB/c 마우스에 각 그룹당 6마리씩 투여하였다. #2 내지 p#3 벡터 100μg을 각 50㎕의 PBS용액(pH 7.4)에 희석하여 근육주사로 투여하였다. 투여 후 마우스에서 2 내지 4주차 시기에 안와 채혈로 약 300㎕의 혈액 시료를 채취한 후 원심분리(8000rpm, 10분, 20℃)를 통해 혈장을 분리하여, 혈액 내에 존재하는 중화 항체의 양을 효소결합면역흡착검사(ELISA) 및 슈도바이러스 중화 분석(Pseudovirus Neutralization Assay)를 통해 측정하였다.

효소결합면역흡착검사는 96-웰 플레이트에 스파이크 단백질(Acro Biosystems, Cat. #: SPN-C52H84)을 PBSN(PBS 1L + Sodium Azide 0.01 g)에 녹여 100 ng/웰로 코팅해준 다음, 16시간 동안 4℃에서 냉장 반응시켰다. 96-웰 플레이트 코팅 16시간 후 코팅 단백질은 제거하고 PBS로 3회 세척한 뒤, 150㎕ 차단 버퍼(Blocking buffer, PBSN+BSA 1%)를 각 웰에 넣고 반응(37℃, 90분)시키고, 반응시간 동안 희석 버퍼(Dilution buffer. PBSN+0.1% BSA+0.05% Tween-20)에 혈장을 6400배 희석하고, 차단 반응이 끝난 후 플레이트를 PBS로 3회 세척 후 희석한 혈장 샘플을 50㎕씩 웰에 넣고 반응(37℃, 3시간)시켰다. 반응 이후, PBS 3회 세척 및 2차 항체인 GAM-IgG-HRP(southernbiotech, Cat. #: 1030-05), GAM-IgM-HRP(southernbiotech, Cat. #: 1020-05)을 희석 버퍼에 1000배 희석하여 50㎕씩 웰에 넣고 반응(37℃, 2시간)한다. 이후 2차 항체를 제거하고 PBS 5회 세척 후, 발색 시약(TMB Peroxidase Substrate buffer, ROCKLAND, Cat. #: TMBE-1000)을 50 ㎕씩 넣고 15분간 발색 시키고 0.25N HCl을 50㎕ 넣어 발색을 멈추고, 시료를 마이크로리더기로 450nm 파장에서 분석하였다.

슈도바이러스 중화 분석은 1×10⁴ 세포/웰의 HEK293T-hACE2(인간 안지오텐신 전환효소 2; hACE2) 세포주를 96-웰 플레이트의 각 웰에 넣고 10시간 배양(37℃, 5% CO₂)하였다. 면역화 후 2 내지 4주차에 마우스의 정맥채혈을 통해 채취한 혈장은 100~6400배(4 배수) 희석하여 7×10⁵ TU/㎖의 슈도바이러스(스파이크 단백질과 루시퍼레이즈를 발현하는 렌티바이러스)와 함께 1시간 반응(37℃)시킨 후, 배양 중인 HEK293T-hACE2 96-웰 플레이트에 3일간 배양하였다. 이후 스파이크 단백질에 결합하는 혈장 내 중화 항체 존재 여부는 슈도바이러스가 HEK293T-hACE2 세포주에 감염되어 루시퍼레이즈 단백질을 발현하는 정도를 측정하여 확인하였다. 3일간 배양한 세포는 DPBS 워싱 후 25㎍ 세포 용해 시약(Promega, Cat. #: E153A)으로 용해시킨다. 용해된 시료는 불투명 96-웰 플레이트에 옮긴 후 100㎍ 루시퍼레이즈 검출 지시약 (Promega, Cat. #: E151A)을 넣고 루미노미터(Luminometer Centro XS3 LB960, Berthold Technologies, Cat. #: LB960, software: Mikro 2000 program)를 이용하여 발광 정도를 측정(검출 지시약 주입-5초 섞임-2초 지연-10초 측정)하여 분석하였다.

<110> Cellid Co.,Ltd. <120> A vector comprising polynucleotide encoding SARS-CoV2 recombinant spike protein and vaccine composition for preventing COVID-19 comprising the same <130> 2021P-08-021 <150> KR 10-2020-0108276 <151> 2020-08-27 <160> 30 <170> KoPatentIn 3.0 <210> 1 <211> 34030 <212> DNA <213> Artificial Sequence <220> <223> pAdk35F <400> 1 ttaattaaca tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 60 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 120 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 180 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 240 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 300 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 360 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 420 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 480 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 540 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 600 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 660 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 720 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 780 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 840 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 900 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 960 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1020 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1080 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1140 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1200 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1260 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1320 ttgttgccat tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca 1380 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 1440 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 1500 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 1560 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 1620 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 1680 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1740 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1800 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1860 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1920 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1980 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2040 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2100 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2160 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 2220 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2280 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2340 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2400 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2460 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 2520 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2580 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 2640 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 2700 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 2760 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 2820 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2880 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 2940 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 3000 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 3060 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 3120 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 3180 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 3240 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta 3300 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 3360 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 3420 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 3480 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 3540 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 3600 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 3660 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 3720 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3780 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3840 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 3900 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 3960 gacaccggga ccgatccagc ctccgattta aattgatcat aatcagccat accacatttg 4020 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 4080 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 4140 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4200 ccaaactcat caatgtatct taacgcggat ctgggcgtgg ttaagggtgg gaaagaatat 4260 ataaggtggg ggtcttatgt agttttgtat ctgttttgca gcagccgccg ccgccatgag 4320 caccaactcg tttgatggaa gcattgtgag ctcatatttg acaacgcgca tgcccccatg 4380 ggccggggtg cgtcagaatg tgatgggctc cagcattgat ggtcgccccg tcctgcccgc 4440 aaactctact accttgacct acgagaccgt gtctggaacg ccgttggaga ctgcagcctc 4500 cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact ttgctttcct 4560 gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca agttgacggc 4620 tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc agcagctgtt 4680 ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa 4740 cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt gctgtcttta 4800 tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct 4860 gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca tgggcataag 4920 cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg tggtgttgta 4980 gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa 5040 gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa gctgggatgg 5100 gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg ctatgttccc 5160 agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt atccggtgca 5220 cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg agacgccctt 5280 gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc cacgggcggc 5340 ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca ggatgagatc 5400 gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta taatggttcc 5460 atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt tgagttcaga 5520 tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg taggggagat 5580 cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg tgggcccgta 5640 aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc cgtcatccct 5700 gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc tgaccaaatc 5760 cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa agtttttcaa 5820 cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca gttccaggcg 5880 gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc ctcgtttcgc 5940 gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg ggccagggtc 6000 atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc 6060 gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc 6120 tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc atagtccagc 6180 ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg 6240 cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc cggggagtag 6300 gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt gagctctggc 6360 cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt acctctggtt 6420 tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac 6480 ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa ctcggaccac 6540 tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga ggggtagcgg 6600 tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc gccctcttcg 6660 gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt tcctgaaggg 6720 gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc gctgtctgcg 6780 agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc tgcgctaaga 6840 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg 6900 agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag cttggtggca 6960 aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt ttggtttttg 7020 tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg cgcaacgcac 7080 cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg ccaaccgcgg 7140 ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg ctcgttggtc 7200 cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag ctgcgtctcg 7260 tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc gaagtagtct 7320 atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag cgcgcgctcg 7380 tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc gtacatgccg 7440 caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg gtagcatctt 7500 ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc gaggaggtcg 7560 ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct gaagatggca 7620 tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc tgtgagacct 7680 accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag ctcggcggtg 7740 acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata cttatcctgt 7800 cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt ccagtactct 7860 tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa ctggttgacg 7920 gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc ggccttccgg 7980 agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga ctttgaggta ctggtatttg 8040 aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa agtccgtgcg ctttttggaa 8100 cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta tctttcccgc gcgaggcata 8160 aagttgcgtg tgatgcggaa gggtcccggc acctcggaac ggttgttaat tacctgggcg 8220 gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca caatgtaaag ttccaagaag 8280 cgcgggatgc ccttgatgga aggcaatttt ttaagttcct cgtaggtgag ctcttcaggg 8340 gagctgagcc cgtgctctga aagggcccag tctgcaagat gagggttgga agcgacgaat 8400 gagctccaca ggtcacgggc cattagcatt tgcaggtggt cgcgaaaggt cctaaactgg 8460 cgacctatgg ccattttttc tggggtgatg cagtagaagg taagcgggtc ttgttcccag 8520 cggtcccatc caaggttcgc ggctaggtct cgcgcggcag tcactagagg ctcatctccg 8580 ccgaacttca tgaccagcat gaagggcacg agctgcttcc caaaggcccc catccaagta 8640 taggtctcta catcgtaggt gacaaagaga cgctcggtgc gaggatgcga gccgatcggg 8700 aagaactgga tctcccgcca ccaattggag gagtggctat tgatgtggtg aaagtagaag 8760 tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa aacgtgcgca gtactggcag 8820 cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac gaccgcgcac aaggaagcag 8880 agtgggaatt tgagcccctc gcctggcggg tttggctggt ggtcttctac ttcggctgct 8940 tgtccttgac cgtctggctg ctcgagggga gttacggtgg atcggaccac cacgccgcgc 9000 gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct tgatgacaac atcgcgcaga 9060 tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt caggcgggag ctcctgcagg 9120 tttacctcgc atagacgggt cagggcgcgg gctagatcca ggtgatacct aatttccagg 9180 ggctggttgg tggcggcgtc gatggcttgc aagaggccgc atccccgcgg cgcgactacg 9240 gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg atgatgcatc taaaagcggt 9300 gacgcgggcg agcccccgga ggtagggggg gctccggacc cgccgggaga gggggcaggg 9360 gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc gcgtaggttg ctggcgaacg 9420 cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg cgtgaagacg acgggcccgg 9480 tgagcttgaa cctgaaagag agttcgacag aatcaatttc ggtgtcgttg acggcggcct 9540 ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata ggcgatctcg gccatgaact 9600 gctcgatctc ttcctcctgg agatctccgc gtccggctcg ctccacggtg gcggcgaggt 9660 cgttggaaat gcgggccatg agctgcgaga aggcgttgag gcctccctcg ttccagacgc 9720 ggctgtagac cacgccccct tcggcatcgc gggcgcgcat gaccacctgc gcgagattga 9780 gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg ctgaaagagg tagttgaggg 9840 tggtggcggt gtgttctgcc acgaagaagt acataaccca gcgtcgcaac gtggattcgt 9900 tgatatcccc caaggcctca aggcgctcca tggcctcgta gaagtccacg gcgaagttga 9960 aaaactggga gttgcgcgcc gacacggtta actcctcctc cagaagacgg atgagctcgg 10020 cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc ctcttcttct tcttcaatct 10080 cctcttccat aagggcctcc ccttcttctt cttctggcgg cggtggggga ggggggacac 10140 ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg ctcgatcatc tccccgcggc 10200 gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg ggggcgcagt tggaagacgc 10260 cgcccgtcat gtcccggtta tgggttggcg gggggctgcc atgcggcagg gatacggcgc 10320 taacgatgca tctcaacaat tgttgtgtag gtactccgcc gccgagggac ctgagcgagt 10380 ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca cagtcgcaag 10440 gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc ggggttgttt ctggcggagg 10500 tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc gacagaagca 10560 ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc ggccatgccc caggcttcgt 10620 tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag cctttctacc ggcacttctt 10680 cttctccttc ctcttgtcct gcatctcttg catctatcgc tgcggcggcg gcggagtttg 10740 gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc gaagcccctc atcggctgaa 10800 gcagggctag gtcggcgaca acgcgctcgg ctaatatggc ctgctgcacc tgcgtgaggg 10860 tagactggaa gtcatccatg tccacaaagc ggtggtatgc gcccgtgttg atggtgtaag 10920 tgcagttggc cataacggac cagttaacgg tctggtgacc cggctgcgag agctcggtgt 10980 acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc gttgcaagtc cgcaccaggt 11040 actggtatcc caccaaaaag tgcggcggcg gctggcggta gaggggccag cgtagggtgg 11100 ccggggctcc gggggcgaga tcttccaaca taaggcgatg atatccgtag atgtacctgg 11160 acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg acgcggttcc 11220 agatgttgcg cagcggcaaa aagtgctcca tggtcgggac gctctggccg gtcaggcgcg 11280 cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg 11340 tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgag ccccgtatcc 11400 ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc 11460 agacaacggg ggagtgctcc ttttggcttc cttccaggcg cggcggctgc tgcgctagct 11520 tttttggcca ctggccgcgc gcagcgtaag cggttaggct ggaaagcgaa agcattaagt 11580 ggctcgctcc ctgtagccgg agggttattt tccaagggtt gagtcgcggg acccccggtt 11640 cgagtctcgg accggccgga ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc 11700 ccgcttgcaa attcctccgg aaacagggac gagccccttt tttgcttttc ccagatgcat 11760 ccggtgctgc ggcagatgcg cccccctcct cagcagcggc aagagcaaga gcagcggcag 11820 acatgcaggg caccctcccc tcctcctacc gcgtcaggag gggcgacatc cgcggttgac 11880 gcggcagcag atggtgatta cgaacccccg cggcgccggg cccggcacta cctggacttg 11940 gaggagggcg agggcctggc gcggctagga gcgccctctc ctgagcggca cccaagggtg 12000 cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc 12060 gagggagagg agcccgagga gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg 12120 catggcctga atcgcgagcg gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc 12180 gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc tggtaaccgc atacgagcag 12240 acggtgaacc aggagattaa ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg 12300 gcgcgcgagg aggtggctat aggactgatg catctgtggg actttgtaag cgcgctggag 12360 caaaacccaa atagcaagcc gctcatggcg cagctgttcc ttatagtgca gcacagcagg 12420 gacaacgagg cattcaggga tgcgctgcta aacatagtag agcccgaggg ccgctggctg 12480 ctcgatttga taaacatcct gcagagcata gtggtgcagg agcgcagctt gagcctggct 12540 gacaaggtgg ccgccatcaa ctattccatg cttagcctgg gcaagtttta cgcccgcaag 12600 atataccata ccccttacgt tcccatagac aaggaggtaa agatcgaggg gttctacatg 12660 cgcatggcgc tgaaggtgct taccttgagc gacgacctgg gcgtttatcg caacgagcgc 12720 atccacaagg ccgtgagcgt gagccggcgg cgcgagctca gcgaccgcga gctgatgcac 12780 agcctgcaaa gggccctggc tggcacgggc agcggcgata gagaggccga gtcctacttt 12840 gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg ccctggaggc agctggggcc 12900 ggacctgggc tggcggtggc acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat 12960 gacgaggacg atgagtacga gccagaggac ggcgagtact aagcggtgat gtttctgatc 13020 agatgatgca agacgcaacg gacccggcgg tgcgggcggc gctgcagagc cagccgtccg 13080 gccttaactc cacggacgac tggcgccagg tcatggaccg catcatgtcg ctgactgcgc 13140 gcaatcctga cgcgttccgg cagcagccgc aggccaaccg gctctccgca attctggaag 13200 cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc 13260 tggccgaaaa cagggccatc cggcccgacg aggccggcct ggtctacgac gcgctgcttc 13320 agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa cctggaccgg ctggtggggg 13380 atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca 13440 tggttgcact aaacgccttc ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg 13500 actacaccaa ctttgtgagc gcactgcggc taatggtgac tgagacaccg caaagtgagg 13560 tgtaccagtc tgggccagac tattttttcc agaccagtag acaaggcctg cagaccgtaa 13620 acctgagcca ggctttcaaa aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg 13680 accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag 13740 cgcccttcac ggacagtggc agcgtgtccc gggacacata cctaggtcac ttgctgacac 13800 tgtaccgcga ggccataggt caggcgcatg tggacgagca tactttccag gagattacaa 13860 gtgtcagccg cgcgctgggg caggaggaca cgggcagcct ggaggcaacc ctaaactacc 13920 tgctgaccaa ccggcggcag aagatcccct cgttgcacag tttaaacagc gaggaggagc 13980 gcattttgcg ctacgtgcag cagagcgtga gccttaacct gatgcgcgac ggggtaacgc 14040 ccagcgtggc gctggacatg accgcgcgca acatggaacc gggcatgtat gcctcaaacc 14100 ggccgtttat caaccgccta atggactact tgcatcgcgc ggccgccgtg aaccccgagt 14160 atttcaccaa tgccatcttg aacccgcact ggctaccgcc ccctggtttc tacaccgggg 14220 gattcgaggt gcccgagggt aacgatggat tcctctggga cgacatagac gacagcgtgt 14280 tttccccgca accgcagacc ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc 14340 tgcgaaagga aagcttccgc aggccaagca gcttgtccga tctaggcgct gcggccccgc 14400 ggtcagatgc tagtagccca tttccaagct tgatagggtc tcttaccagc actcgcacca 14460 cccgcccgcg cctgctgggc gaggaggagt acctaaacaa ctcgctgctg cagccgcagc 14520 gcgaaaaaaa cctgcctccg gcatttccca acaacgggat agagagccta gtggacaaga 14580 tgagtagatg gaagacgtac gcgcaggagc acagggacgt gccaggcccg cgcccgccca 14640 cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg ggaggacgat gactcggcag 14700 acgacagcag cgtcctggat ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca 14760 ggctggggag aatgttttaa aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg 14820 ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg cggcgatgta 14880 tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag tggcggcggc 14940 gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg 15000 gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat tcgacaccac 15060 ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact accagaacga 15120 ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg gggaggcaag 15180 cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga aaaccatcct 15240 gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta aggcgcgggt 15300 gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga 15360 gttcacgctg cccgagggca actactccga gaccatgacc atagacctta tgaacaacgc 15420 gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa gcgacatcgg 15480 ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg gtcttgtcat 15540 gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc caggatgcgg 15600 ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt 15660 ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc ccgcactgtt 15720 ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg ggggtggcgc 15780 aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc 15840 aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct ttgccacacg 15900 ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc ccgctgcgca 15960 acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag aggacagcaa 16020 gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc gcagctggta 16080 ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc tgctttgcac 16140 tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca tgatgcaaga 16200 ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg gcgccgagct 16260 gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc aactcatccg 16320 ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg 16380 cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg 16440 gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta ctgacgccag 16500 acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc gcgtcctatc 16560 gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata acacaggctg 16620 gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg accaacaccc 16680 agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg 16740 gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca actacacgcc 16800 cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc gcggagcccg 16860 gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc gccgccgacc 16920 cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg 16980 acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg tgccccccag 17040 gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga ctcagggtcg 17100 caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac 17160 ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact gttgtatgta 17220 tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag aagagatgct 17280 ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg attacaagcc 17340 ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga 17400 ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt 17460 aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc gctccacccg 17520 cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg agcaggccaa 17580 cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg cgttgccgct 17640 ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc 17700 gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact tggcacccac 17760 cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa aaatgaccgt 17820 ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg cgccgggact 17880 gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta ttgccaccgc 17940 cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt 18000 gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg acccgtggat 18060 gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg ccgccagcgc 18120 gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct atcgtggcta 18180 cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg gaacccgccg 18240 ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga 18300 aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg tttaaaagcc 18360 ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc cggtgccggg 18420 attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga cgggcggcat 18480 gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct 18540 gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt 18600 ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa atcaaaataa 18660 aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg gaagacatca 18720 actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac tggcaagata 18780 tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta 18840 aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc agcacaggcc 18900 agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta gatggcctgg 18960 cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat aagattaaca 19020 gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag acagtgtctc 19080 cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa 19140 tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc acccgtccca 19200 tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg gacctgcctc 19260 cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc 19320 gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag 19380 ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg caatccctga 19440 agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat gcgtccatgt 19500 cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc taccccttcg 19560 atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga gtacctgagc 19620 cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa taacaagttt 19680 agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca gcgtttgacg 19740 ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc gcggttcacc 19800 ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga catccgcggc 19860 gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa cgccctggct 19920 cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct tgaaataaac 19980 ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga gcagcaaaaa 20040 actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga gggtattcaa 20100 ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc tgaacctcaa 20160 ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag agtcctaaaa 20220 aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa tgaaaatgga 20280 gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt ggaaatgcaa 20340 tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc taaagtggta 20400 ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta catgcccact 20460 attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa caggcctaat 20520 tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac gggtaatatg 20580 ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca agacagaaac 20640 acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag gtacttttct 20700 atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga aaatcatgga 20760 actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa tacagagact 20820 cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga tgctacagaa 20880 ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat caatctaaat 20940 gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc cgacaagcta 21000 aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga ctacatgaac 21060 aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc acgctggtcc 21120 cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg cctgcgctac 21180 cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt gcctcagaag 21240 ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga gtggaacttc 21300 aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag ggttgacgga 21360 gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat ggcccacaac 21420 accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc ctttaacgac 21480 tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa cgtgcccata 21540 tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg ccttaagact 21600 aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc tggctctata 21660 ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc cattaccttt 21720 gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga gtttgaaatt 21780 aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac caaagactgg 21840 ttcctggtac aaatgctagc taactataac attggctacc agggcttcta tatcccagag 21900 agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag ccgtcaggtg 21960 gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca acacaacaac 22020 tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta ccctgctaac 22080 ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa aaagtttctt 22140 tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat gggcgcactc 22200 acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga catgactttt 22260 gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt ctttgacgtg 22320 gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg cacgcccttc 22380 tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct gccgccatgg 22440 gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg ccatattttt 22500 tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc gcctgcgcca 22560 tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt gcctggaacc 22620 cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag cgactcaagc 22680 aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct tcttcccccg 22740 accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac tcggccgcct 22800 gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa actcccatgg 22860 atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc aacagtcccc 22920 aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg gagcgccact 22980 cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt tgtcacttga 23040 aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc ttttatttgt 23100 acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa aaatcaaagg 23160 ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac tggtgtttag 23220 tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt tcactccaca 23280 ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg aagtcgcagt 23340 tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac tggaacacta 23400 tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga tccgcgtcca 23460 ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt cccaaaaagg 23520 gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg tgaccgtgcc 23580 cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta aaagccacct 23640 gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac tgattggccg 23700 gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc accacatttc 23760 ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc gcgcgctgcc 23820 cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata atgcttccgt 23880 gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac gcgcagcccg 23940 tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc tgcaggaatc 24000 gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac ccgcggtgct 24060 cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca ggcagtagtt 24120 tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg cgcgcagcct 24180 ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc accgtaattt 24240 cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca cgcgccactg 24300 ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc ttgattagca 24360 ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct tcctcgctgt 24420 ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc ttctttttct 24480 tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg ggtgtgcgcg 24540 gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc cgcctcatcc 24600 gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac acgtcctcca 24660 tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg cgctgctcct 24720 cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag tcagtcgaga 24780 agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc gatgccgcca 24840 acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa gtgattatcg 24900 agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca acagaggata 24960 aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg ggggacgaaa 25020 ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg cagcgccagt 25080 gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc atagcggatg 25140 tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa cgccaagaaa 25200 acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc gtgccagagg 25260 tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc tgccgtgcca 25320 accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata cctgatatcg 25380 cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag aagcgcgcgg 25440 caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg ttggtggaac 25500 tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc acccactttg 25560 cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt gagctgatcg 25620 tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca gaggagggcc 25680 tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag cctgccgact 25740 tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag cttgagtgca 25800 tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca ttgcactaca 25860 cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag ctctgcaacc 25920 tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg cttcattcca 25980 cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta tttctatgct 26040 acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc aacctcaagg 26100 agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc aacgagcgct 26160 ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa accctgcaac 26220 agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac tttatcctag 26280 agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt gtgcccatta 26340 agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag ctagccaact 26400 accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta ctggagtgtc 26460 actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg cagctgctta 26520 acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac gaaaagtccg 26580 cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt cgcaaatttg 26640 tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc cgcccgccta 26700 atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa ttgcaagcca 26760 tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac ttggaccccc 26820 agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag cagcagccgc 26880 gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc gccacccacg 26940 gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga ggaggaggac 27000 atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga ggtgtcagac 27060 gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc ggcaaccggt 27120 tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt tcgccgaccc 27180 aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc gccgccgtta 27240 gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa gaacgccata 27300 gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt tcttctctac 27360 catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct ctacagccca 27420 tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga 27480 tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc 27540 gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc 27600 cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa 27660 caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg 27720 gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga 27780 ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca 27840 cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca cgccctacat 27900 gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact actcaacccg 27960 aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa tacgcgccca 28020 ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta ataaccttaa 28080 tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cccgctccca ccactgtggt 28140 acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc agcttgcggg 28200 cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga caatcagagg 28260 gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc gtccggacgg 28320 gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg caatcctaac 28380 tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc aatttattga 28440 ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc actatccgga 28500 tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg actgaatgtt 28560 aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc gccacaagtg 28620 ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc atatcgaggg 28680 cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc tgattcggga 28740 gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc tcactgtgat 28800 ttgcaactgt cctaaccctg gattacatca agatcctcta gttaatgtca ggtcgcctaa 28860 gtcgattaac tagagtaccc ggggatctta ttccctttaa ctaataaaaa aaaataataa 28920 agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag cagcacctcc 28980 ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt tctccacaat 29040 ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat cttcatgttg 29100 ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta tccatatgac 29160 acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc ccccaatggg 29220 tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct agttacctcc 29280 aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc cggcaacctt 29340 acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc aaacataaac 29400 ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc tgccgccgca 29460 cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct aaccgtgcac 29520 gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg aaagctagcc 29580 ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat cactgcctca 29640 ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc catttataca 29700 caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga cgacctaaac 29760 actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt gcaaactaaa 29820 gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt agcaggagga 29880 ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc gtttgatgct 29940 caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc agcccacaac 30000 ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa ttccaaaaag 30060 cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc catagccatt 30120 aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa tcccctcaaa 30180 acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc taaactagga 30240 actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa taatgataag 30300 ctaactttgt ggaccggaat aaaccctcca cctaactgtc aaattgtgga aaacactaat 30360 acaaatgatg gcaaacttac tttagtatta gtaaaaaatg gagggcttgt taatggctac 30420 gtgtctctag ttggtgtatc agacactgtg aaccaaatgt tcacacaaaa gacagcaaac 30480 atccaattaa gattatattt tgactcttct ggaaatctat taactgagga atcagactta 30540 aaaattccac ttaaaaataa atcttctaca gcgaccagtg aaactgtagc cagcagcaaa 30600 gcctttatgc caagtactac agcttatccc ttcaacacca ctactaggga tagtgaaaac 30660 tacattcatg gaatatgtta ctacatgact agttatgata gaagtctatt tcccttgaac 30720 atttctataa tgctaaacag ccgtatgatt tcttccaatg ttgcctatgc catacaattt 30780 gaatggaatc taaatgcaag tgaatctcca gaaagcaaca tagctacgct gaccacatcc 30840 ccctttttct tttcttacat tacagaagac gacaactaaa gaatcgtttg tgttatgttt 30900 caacgtgttt atttttcaat tgcagaaaat ttcaagtcat ttttcattca gtagtatagc 30960 cccaccacca catagcttat acagatcacc gtaccttaat caaactcaca gaaccctagt 31020 attcaacctg ccacctccct cccaacacac agagtacaca gtcctttctc cccggctggc 31080 cttaaaaagc atcatatcat gggtaacaga catattctta ggtgttatat tccacacggt 31140 ttcctgtcga gccaaacgct catcagtgat attaataaac tccccgggca gctcacttaa 31200 gttcatgtcg ctgtccagct gctgagccac aggctgctgt ccaacttgcg gttgcttaac 31260 gggcggcgaa ggagaagtcc acgcctacat gggggtagag tcataatcgt gcatcaggat 31320 agggcggtgg tgctgcagca gcgcgcgaat aaactgctgc cgccgccgct ccgtcctgca 31380 ggaatacaac atggcagtgg tctcctcagc gatgattcgc accgcccgca gcataaggcg 31440 ccttgtcctc cgggcacagc agcgcaccct gatctcactt aaatcagcac agtaactgca 31500 gcacagcacc acaatattgt tcaaaatccc acagtgcaag gcgctgtatc caaagctcat 31560 ggcggggacc acagaaccca cgtggccatc ataccacaag cgcaggtaga ttaagtggcg 31620 acccctcata aacacgctgg acataaacat tacctctttt ggcatgttgt aattcaccac 31680 ctcccggtac catataaacc tctgattaaa catggcgcca tccaccacca tcctaaacca 31740 gctggccaaa acctgcccgc cggctataca ctgcagggaa ccgggactgg aacaatgaca 31800 gtggagagcc caggactcgt aaccatggat catcatgctc gtcatgatat caatgttggc 31860 acaacacagg cacacgtgca tacacttcct caggattaca agctcctccc gcgttagaac 31920 catatcccag ggaacaaccc attcctgaat cagcgtaaat cccacactgc agggaagacc 31980 tcgcacgtaa ctcacgttgt gcattgtcaa agtgttacat tcgggcagca gcggatgatc 32040 ctccagtatg gtagcgcggg tttctgtctc aaaaggaggt agacgatccc tactgtacgg 32100 agtgcgccga gacaaccgag atcgtgttgg tcgtagtgtc atgccaaatg gaacgccgga 32160 cgtagtcata tttcctgaag caaaaccagg tgcgggcgtg acaaacagat ctgcgtctcc 32220 ggtctcgccg cttagatcgc tctgtgtagt agttgtagta tatccactct ctcaaagcat 32280 ccaggcgccc cctggcttcg ggttctatgt aaactccttc atgcgccgct gccctgataa 32340 catccaccac cgcagaataa gccacaccca gccaacctac acattcgttc tgcgagtcac 32400 acacgggagg agcgggaaga gctggaagaa ccatgttttt ttttttattc caaaagatta 32460 tccaaaacct caaaatgaag atctattaag tgaacgcgct cccctccggt ggcgtggtca 32520 aactctacag ccaaagaaca gataatggca tttgtaagat gttgcacaat ggcttccaaa 32580 aggcaaacgg ccctcacgtc caagtggacg taaaggctaa acccttcagg gtgaatctcc 32640 tctataaaca ttccagcacc ttcaaccatg cccaaataat tctcatctcg ccaccttctc 32700 aatatatctc taagcaaatc ccgaatatta agtccggcca ttgtaaaaat ctgctccaga 32760 gcgccctcca ccttcagcct caagcagcga atcatgattg caaaaattca ggttcctcac 32820 agacctgtat aagattcaaa agcggaacat taacaaaaat accgcgatcc cgtaggtccc 32880 ttcgcagggc cagctgaaca taatcgtgca ggtctgcacg gaccagcgcg gccacttccc 32940 cgccaggaac catgacaaaa gaacccacac tgattatgac acgcatactc ggagctatgc 33000 taaccagcgt agccccgatg taagcttgtt gcatgggcgg cgatataaaa tgcaaggtgc 33060 tgctcaaaaa atcaggcaaa gcctcgcgca aaaaagaaag cacatcgtag tcatgctcat 33120 gcagataaag gcaggtaagc tccggaacca ccacagaaaa agacaccatt tttctctcaa 33180 acatgtctgc gggtttctgc ataaacacaa aataaaataa caaaaaaaca tttaaacatt 33240 agaagcctgt cttacaacag gaaaaacaac ccttataagc ataagacgga ctacggccat 33300 gccggcgtga ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg acagctcctc 33360 ggtcatgtcc ggagtcataa tgtaagactc ggtaaacaca tcaggttgat tcacatcggt 33420 cagtgctaaa aagcgaccga aatagcccgg gggaatacat acccgcaggc gtagagacaa 33480 cattacagcc cccataggag gtataacaaa attaatagga gagaaaaaca cataaacacc 33540 tgaaaaaccc tcctgcctag gcaaaatagc accctcccgc tccagaacaa catacagcgc 33600 ttccacagcg gcagccataa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa 33660 acaccactcg acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga 33720 gcgagtatat ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga 33780 aaaccgcacg cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat 33840 cgtcacttcc gttttcccac gttacgtcac ttcccatttt aagaaaacta caattcccaa 33900 cacatacaag ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc 33960 cacgtcacaa actccacccc ctcattatca tattggcttc aatccaaaat aaggtatatt 34020 attgatgatg 34030 <210> 2 <211> 9858 <212> DNA <213> Artificial Sequence <220> <223> pCMV3-SARS-CoV2(Spike ORF) <400> 2 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg agtacattta tattggctca tgtccaatat 240 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 300 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 360 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 420 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 480 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta 540 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt 600 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg 660 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 720 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc 780 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt 840 tagtgaaccg tcagatcctc actctcttcc gcatcgctgt ctgcgagggc cagctgttgg 900 gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc ggaaacccgt 960 cggcctccga acggtactcc gccaccgagg gacctgagcg agtccgcatc gaccggatcg 1020 gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg 1080 gcgggcggca gcgggtggcg gtcggggttg tttctggcgg aggtgctgct gatgatgtaa 1140 ttaaagtagg cggtcttgag acggcggatg gtcgaggtga ggtgtggcag gcttgagatc 1200 cagctgttgg ggtgagtact ccctctcaaa agcgggcatt acttctgcgc taagattgtc 1260 agtttccaaa aacgaggagg atttgatatt cacctggccc gatctggcca tacacttgag 1320 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaagttta 1380 aactttaata cgactcacta taggggccgc caccaagctt ggtaccatgt ttgtgttcct 1440 ggtgctgctg ccactggtgt ccagccagtg tgtgaacctg accaccagga cccaacttcc 1500 tcctgcctac accaactcct tcaccagggg agtctactac cctgacaagg tgttcaggtc 1560 ctctgtgctg cacagcaccc aggacctgtt cctgccattc ttcagcaatg tgacctggtt 1620 ccatgccatc catgtgtctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc 1680 attcaatgat ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat 1740 ttttggcacc accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa 1800 tgtggtgatt aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg gagtctacta 1860 ccacaagaac aacaagtcct ggatggagtc tgagttcagg gtctactcct ctgccaacaa 1920 ctgtaccttt gaatatgtga gccaaccatt cctgatggac ttggagggca agcagggcaa 1980 cttcaagaac ctgagggagt ttgtgttcaa gaacattgat ggctacttca agatttacag 2040 caaacacaca ccaatcaacc tggtgaggga cctgccacag ggcttctctg ccttggaacc 2100 actggtggac ctgccaattg gcatcaacat caccaggttc cagaccctgc tggctctgca 2160 caggtcctac ctgacacctg gagactcctc ctctggctgg acagcaggag cagcagccta 2220 ctatgtgggc tacctccaac caaggacctt cctgctgaaa tacaatgaga atggcaccat 2280 cacagatgct gtggactgtg ccctggaccc actgtctgag accaagtgta ccctgaaatc 2340 cttcacagtg gagaagggca tctaccagac cagcaacttc agggtccaac caacagagag 2400 cattgtgagg tttccaaaca tcaccaacct gtgtccattt ggagaggtgt tcaatgccac 2460 caggtttgcc tctgtctatg cctggaacag gaagaggatt agcaactgtg tggctgacta 2520 ctctgtgctc tacaactctg cctccttcag caccttcaag tgttatggag tgagcccaac 2580 caaactgaat gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga ttaggggaga 2640 tgaggtgaga cagattgccc ctggacaaac aggcaagatt gctgactaca actacaaact 2700 gcctgatgac ttcacaggct gtgtgattgc ctggaacagc aacaacctgg acagcaaggt 2760 gggaggcaac tacaactacc tctacagact gttcaggaag agcaacctga aaccatttga 2820 gagggacatc agcacagaga tttaccaggc tggcagcaca ccatgtaatg gagtggaggg 2880 cttcaactgt tactttccac tccaatccta tggcttccaa ccaaccaatg gagtgggcta 2940 ccaaccatac agggtggtgg tgctgtcctt tgaactgctc catgcccctg ccacagtgtg 3000 tggaccaaag aagagcacca acctggtgaa gaacaagtgt gtgaacttca acttcaatgg 3060 actgacaggc acaggagtgc tgacagagag caacaagaag ttcctgccat tccaacagtt 3120 tggcagggac attgctgaca ccacagatgc tgtgagggac ccacagacct tggagattct 3180 ggacatcaca ccatgttcct ttggaggagt gtctgtgatt acacctggca ccaacaccag 3240 caaccaggtg gctgtgctct accaggatgt gaactgtact gaggtgcctg tggctatcca 3300 tgctgaccaa cttacaccaa cctggagggt ctacagcaca ggcagcaatg tgttccagac 3360 cagggctggc tgtctgattg gagcagagca tgtgaacaac tcctatgagt gtgacatccc 3420 aattggagca ggcatctgtg cctcctacca gacccagacc atcctcaggt ctgtggcaag 3480 ccagagcatc attgcctaca caatgagtct gggagcagag aactctgtgg cttacagcaa 3540 caacagcatt gccatcccaa ccaacttcac catctctgtg accacagaga ttctgcctgt 3600 gagtatgacc aagacctctg tggactgtac aatgtatatc tgtggagaca gcacagagtg 3660 tagcaacctg ctgctccaat atggctcctt ctgtacccaa cttaacaggg ctctgacagg 3720 cattgctgtg gaacaggaca agaacaccca ggaggtgttt gcccaggtga agcagattta 3780 caagacacct ccaatcaagg actttggagg cttcaacttc agccagattc tgcctgaccc 3840 aagcaagcca agcaagaggt ccttcattga ggacctgctg ttcaacaagg tgaccctggc 3900 tgatgctggc ttcatcaagc aatatggaga ctgtctggga gacattgctg ccagggacct 3960 gatttgtgcc cagaagttca atggactgac agtgctgcct ccactgctga cagatgagat 4020 gattgcccaa tacacctctg ccctgctggc tggcaccatc acctctggct ggacctttgg 4080 agcaggagca gccctccaaa tcccatttgc tatgcagatg gcttacaggt tcaatggcat 4140 tggagtgacc cagaatgtgc tctatgagaa ccagaaactg attgccaacc agttcaactc 4200 tgccattggc aagattcagg actccctgtc cagcacagcc tctgccctgg gcaaactcca 4260 agatgtggtg aaccagaatg cccaggctct gaacaccctg gtgaagcaac tttccagcaa 4320 ctttggagcc atctcctctg tgctgaatga catcctgagc agactggaca aggtggaggc 4380 tgaggtccag attgacagac tgattacagg cagactccaa tccctccaaa cctatgtgac 4440 ccaacaactt atcagggctg ctgagattag ggcatctgcc aacctggctg ccaccaagat 4500 gagtgagtgt gtgctgggac aaagcaagag ggtggacttc tgtggcaagg gctaccacct 4560 gatgagtttt ccacagtctg cccctcatgg agtggtgttc ctgcatgtga cctatgtgcc 4620 tgcccaggag aagaacttca ccacagcccc tgccatctgc catgatggca aggctcactt 4680 tccaagggag ggagtgtttg tgagcaatgg cacccactgg tttgtgaccc agaggaactt 4740 ctatgaacca cagattatca ccacagacaa cacctttgtg tctggcaact gtgatgtggt 4800 gattggcatt gtgaacaaca cagtctatga cccactccaa cctgaactgg actccttcaa 4860 ggaggaactg gacaaatact tcaagaacca caccagccct gatgtggacc tgggagacat 4920 ctctggcatc aatgcctctg tggtgaacat ccagaaggag attgacagac tgaatgaggt 4980 ggctaagaac ctgaatgagt ccctgattga cctccaagaa ctgggcaaat atgaacaata 5040 catcaagtgg ccatggtaca tctggctggg cttcattgct ggactgattg ccattgtgat 5100 ggtgaccata atgctgtgtt gtatgacctc ctgttgttcc tgtctgaaag gctgttgttc 5160 ctgtggctcc tgttgtaagt ttgatgagga tgactctgaa cctgtgctga aaggagtgaa 5220 actgcactac acctgatcta gagcggccgc cgaattcggg cccgtttaaa cccgctgatc 5280 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 5340 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 5400 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 5460 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 5520 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 5580 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 5640 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 5700 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 5760 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 5820 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 5880 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 5940 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 6000 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 6060 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 6120 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 6180 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6240 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 6300 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 6360 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt 6420 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct 6480 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc 6540 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt 6600 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt 6660 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg 6720 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc 6780 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt 6840 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc 6900 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc 6960 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc 7020 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc 7080 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg 7140 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc 7200 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac 7260 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg 7320 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc 7380 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc 7440 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 7500 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 7560 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 7620 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 7680 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7740 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7800 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7860 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7920 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7980 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 8040 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8100 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8160 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8220 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8280 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8340 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8400 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8460 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8520 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8580 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8640 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8700 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 8760 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 8820 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 8880 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 8940 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9000 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9060 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9120 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9180 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9240 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9300 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9360 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9420 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9480 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9540 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9600 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9660 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9720 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 9780 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 9840 aaagtgccac ctgacgtc 9858 <210> 3 <211> 3642 <212> DNA <213> Artificial Sequence <220> <223> spike protein coding sequence in #1 vector <400> 3 atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc 60 aggacccaac ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac 120 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc 180 aatgtgacct ggttccatgc catccatgtg tctggcacca atggcaccaa gaggtttgac 240 aaccctgtgc tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc 300 atcaggggct ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg 360 aacaatgcca ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc 420 ctgggagtct actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac 480 tcctctgcca acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag 540 ggcaagcagg gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac 600 ttcaagattt acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc 660 tctgccttgg aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc 720 ctgctggctc tgcacaggtc ctacctgaca cctggagact cctcctctgg ctggacagca 780 ggagcagcag cctactatgt gggctacctc caaccaagga ccttcctgct gaaatacaat 840 gagaatggca ccatcacaga tgctgtggac tgtgccctgg acccactgtc tgagaccaag 900 tgtaccctga aatccttcac agtggagaag ggcatctacc agaccagcaa cttcagggtc 960 caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag 1020 gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac 1080 tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat 1140 ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt 1200 gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac 1260 tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac 1320 ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac 1380 ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt 1440 aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc 1500 aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc 1560 cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac 1620 ttcaacttca atggactgac aggcacagga gtgctgacag agagcaacaa gaagttcctg 1680 ccattccaac agtttggcag ggacattgct gacaccacag atgctgtgag ggacccacag 1740 accttggaga ttctggacat cacaccatgt tcctttggag gagtgtctgt gattacacct 1800 ggcaccaaca ccagcaacca ggtggctgtg ctctaccagg atgtgaactg tactgaggtg 1860 cctgtggcta tccatgctga ccaacttaca ccaacctgga gggtctacag cacaggcagc 1920 aatgtgttcc agaccagggc tggctgtctg attggagcag agcatgtgaa caactcctat 1980 gagtgtgaca tcccaattgg agcaggcatc tgtgcctcct accagaccca gaccaacagc 2040 ccaaggaggg caaggtctgt ggcaagccag agcatcattg cctacacaat gagtctggga 2100 gcagagaact ctgtggctta cagcaacaac agcattgcca tcccaaccaa cttcaccatc 2160 tctgtgacca cagagattct gcctgtgagt atgaccaaga cctctgtgga ctgtacaatg 2220 tatatctgtg gagacagcac agagtgtagc aacctgctgc tccaatatgg ctccttctgt 2280 acccaactta acagggctct gacaggcatt gctgtggaac aggacaagaa cacccaggag 2340 gtgtttgccc aggtgaagca gatttacaag acacctccaa tcaaggactt tggaggcttc 2400 aacttcagcc agattctgcc tgacccaagc aagccaagca agaggtcctt cattgaggac 2460 ctgctgttca acaaggtgac cctggctgat gctggcttca tcaagcaata tggagactgt 2520 ctgggagaca ttgctgccag ggacctgatt tgtgcccaga agttcaatgg actgacagtg 2580 ctgcctccac tgctgacaga tgagatgatt gcccaataca cctctgccct gctggctggc 2640 accatcacct ctggctggac ctttggagca ggagcagccc tccaaatccc atttgctatg 2700 cagatggctt acaggttcaa tggcattgga gtgacccaga atgtgctcta tgagaaccag 2760 aaactgattg ccaaccagtt caactctgcc attggcaaga ttcaggactc cctgtccagc 2820 acagcctctg ccctgggcaa actccaagat gtggtgaacc agaatgccca ggctctgaac 2880 accctggtga agcaactttc cagcaacttt ggagccatct cctctgtgct gaatgacatc 2940 ctgagcagac tggacaaggt ggaggctgag gtccagattg acagactgat tacaggcaga 3000 ctccaatccc tccaaaccta tgtgacccaa caacttatca gggctgctga gattagggca 3060 tctgccaacc tggctgccac caagatgagt gagtgtgtgc tgggacaaag caagagggtg 3120 gacttctgtg gcaagggcta ccacctgatg agttttccac agtctgcccc tcatggagtg 3180 gtgttcctgc atgtgaccta tgtgcctgcc caggagaaga acttcaccac agcccctgcc 3240 atctgccatg atggcaaggc tcactttcca agggagggag tgtttgtgag caatggcacc 3300 cactggtttg tgacccagag gaacttctat gaaccacaga ttatcaccac agacaacacc 3360 tttgtgtctg gcaactgtga tgtggtgatt ggcattgtga acaacacagt ctatgaccca 3420 ctccaacctg aactggactc cttcaaggag gaactggaca aatacttcaa gaaccacacc 3480 agccctgatg tggacctggg agacatctct ggcatcaatg cctctgtggt gaacatccag 3540 aaggagattg acagactgaa tgaggtggct aagaacctga atgagtccct gattgacctc 3600 caagaactgg gcaaatatga acaatacatc aagtggccat ga 3642 <210> 4 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> spike protein ending gene forward primer <400> 4 tccagcctcc gatttgccac catgtttgtg ttcctgg 37 <210> 5 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> spike protein ending gene reverse primer <400> 5 gattatgatc aattttcatg gccacttgat gtattgttc 39 <210> 6 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> S1/S2 cleavage site <400> 6 acgaattctc ctcggcgggc acgt 24 <210> 7 <211> 12 <212> DNA <213> Artificial Sequence <220> <223> linker <400> 7 acgatcctcc gt 12 <210> 8 <211> 3630 <212> DNA <213> Artificial Sequence <220> <223> spike protein coding sequence in #2 vector <400> 8 atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc 60 aggacccaac ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac 120 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc 180 aatgtgacct ggttccatgc catccatgtg tctggcacca atggcaccaa gaggtttgac 240 aaccctgtgc tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc 300 atcaggggct ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg 360 aacaatgcca ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc 420 ctgggagtct actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac 480 tcctctgcca acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag 540 ggcaagcagg gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac 600 ttcaagattt acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc 660 tctgccttgg aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc 720 ctgctggctc tgcacaggtc ctacctgaca cctggagact cctcctctgg ctggacagca 780 ggagcagcag cctactatgt gggctacctc caaccaagga ccttcctgct gaaatacaat 840 gagaatggca ccatcacaga tgctgtggac tgtgccctgg acccactgtc tgagaccaag 900 tgtaccctga aatccttcac agtggagaag ggcatctacc agaccagcaa cttcagggtc 960 caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag 1020 gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac 1080 tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat 1140 ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt 1200 gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac 1260 tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac 1320 ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac 1380 ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt 1440 aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc 1500 aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc 1560 cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac 1620 ttcaacttca atggactgac aggcacagga gtgctgacag agagcaacaa gaagttcctg 1680 ccattccaac agtttggcag ggacattgct gacaccacag atgctgtgag ggacccacag 1740 accttggaga ttctggacat cacaccatgt tcctttggag gagtgtctgt gattacacct 1800 ggcaccaaca ccagcaacca ggtggctgtg ctctaccagg atgtgaactg tactgaggtg 1860 cctgtggcta tccatgctga ccaacttaca ccaacctgga gggtctacag cacaggcagc 1920 aatgtgttcc agaccagggc tggctgtctg attggagcag agcatgtgaa caactcctat 1980 gagtgtgaca tcccaattgg agcaggcatc tgtgcctcct accagaccca gaccatcctc 2040 aggtctgtgg caagccagag catcattgcc tacacaatga gtctgggagc agagaactct 2100 gtggcttaca gcaacaacag cattgccatc ccaaccaact tcaccatctc tgtgaccaca 2160 gagattctgc ctgtgagtat gaccaagacc tctgtggact gtacaatgta tatctgtgga 2220 gacagcacag agtgtagcaa cctgctgctc caatatggct ccttctgtac ccaacttaac 2280 agggctctga caggcattgc tgtggaacag gacaagaaca cccaggaggt gtttgcccag 2340 gtgaagcaga tttacaagac acctccaatc aaggactttg gaggcttcaa cttcagccag 2400 attctgcctg acccaagcaa gccaagcaag aggtccttca ttgaggacct gctgttcaac 2460 aaggtgaccc tggctgatgc tggcttcatc aagcaatatg gagactgtct gggagacatt 2520 gctgccaggg acctgatttg tgcccagaag ttcaatggac tgacagtgct gcctccactg 2580 ctgacagatg agatgattgc ccaatacacc tctgccctgc tggctggcac catcacctct 2640 ggctggacct ttggagcagg agcagccctc caaatcccat ttgctatgca gatggcttac 2700 aggttcaatg gcattggagt gacccagaat gtgctctatg agaaccagaa actgattgcc 2760 aaccagttca actctgccat tggcaagatt caggactccc tgtccagcac agcctctgcc 2820 ctgggcaaac tccaagatgt ggtgaaccag aatgcccagg ctctgaacac cctggtgaag 2880 caactttcca gcaactttgg agccatctcc tctgtgctga atgacatcct gagcagactg 2940 gacaaggtgg aggctgaggt ccagattgac agactgatta caggcagact ccaatccctc 3000 caaacctatg tgacccaaca acttatcagg gctgctgaga ttagggcatc tgccaacctg 3060 gctgccacca agatgagtga gtgtgtgctg ggacaaagca agagggtgga cttctgtggc 3120 aagggctacc acctgatgag ttttccacag tctgcccctc atggagtggt gttcctgcat 3180 gtgacctatg tgcctgccca ggagaagaac ttcaccacag cccctgccat ctgccatgat 3240 ggcaaggctc actttccaag ggagggagtg tttgtgagca atggcaccca ctggtttgtg 3300 acccagagga acttctatga accacagatt atcaccacag acaacacctt tgtgtctggc 3360 aactgtgatg tggtgattgg cattgtgaac aacacagtct atgacccact ccaacctgaa 3420 ctggactcct tcaaggagga actggacaaa tacttcaaga accacaccag ccctgatgtg 3480 gacctgggag acatctctgg catcaatgcc tctgtggtga acatccagaa ggagattgac 3540 agactgaatg aggtggctaa gaacctgaat gagtccctga ttgacctcca agaactgggc 3600 aaatatgaac aatacatcaa gtggccatga 3630 <210> 9 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 9 agaccatcct caggtctgtg gcaagccaga g 31 <210> 10 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 10 cctcctacca gacccagacc atcctcaggt 30 <210> 11 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> linker <400> 11 accggtggcg gtgggtcgag gtctgtg 27 <210> 12 <211> 3639 <212> DNA <213> Artificial Sequence <220> <223> spike protein coding sequence in #3 vector <400> 12 atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc 60 aggacccaac ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac 120 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc 180 aatgtgacct ggttccatgc catccatgtg tctggcacca atggcaccaa gaggtttgac 240 aaccctgtgc tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc 300 atcaggggct ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg 360 aacaatgcca ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc 420 ctgggagtct actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac 480 tcctctgcca acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag 540 ggcaagcagg gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac 600 ttcaagattt acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc 660 tctgccttgg aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc 720 ctgctggctc tgcacaggtc ctacctgaca cctggagact cctcctctgg ctggacagca 780 ggagcagcag cctactatgt gggctacctc caaccaagga ccttcctgct gaaatacaat 840 gagaatggca ccatcacaga tgctgtggac tgtgccctgg acccactgtc tgagaccaag 900 tgtaccctga aatccttcac agtggagaag ggcatctacc agaccagcaa cttcagggtc 960 caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag 1020 gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac 1080 tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat 1140 ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt 1200 gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac 1260 tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac 1320 ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac 1380 ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt 1440 aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc 1500 aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc 1560 cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac 1620 ttcaacttca atggactgac aggcacagga gtgctgacag agagcaacaa gaagttcctg 1680 ccattccaac agtttggcag ggacattgct gacaccacag atgctgtgag ggacccacag 1740 accttggaga ttctggacat cacaccatgt tcctttggag gagtgtctgt gattacacct 1800 ggcaccaaca ccagcaacca ggtggctgtg ctctaccagg atgtgaactg tactgaggtg 1860 cctgtggcta tccatgctga ccaacttaca ccaacctgga gggtctacag cacaggcagc 1920 aatgtgttcc agaccagggc tggctgtctg attggagcag agcatgtgaa caactcctat 1980 gagtgtgaca tcccaattgg agcaggcatc tgtgcctcct accagaccca gaccggtggc 2040 ggtgggtcga ggtctgtggc aagccagagc atcattgcct acacaatgag tctgggagca 2100 gagaactctg tggcttacag caacaacagc attgccatcc caaccaactt caccatctct 2160 gtgaccacag agattctgcc tgtgagtatg accaagacct ctgtggactg tacaatgtat 2220 atctgtggag acagcacaga gtgtagcaac ctgctgctcc aatatggctc cttctgtacc 2280 caacttaaca gggctctgac aggcattgct gtggaacagg acaagaacac ccaggaggtg 2340 tttgcccagg tgaagcagat ttacaagaca cctccaatca aggactttgg aggcttcaac 2400 ttcagccaga ttctgcctga cccaagcaag ccaagcaaga ggtccttcat tgaggacctg 2460 ctgttcaaca aggtgaccct ggctgatgct ggcttcatca agcaatatgg agactgtctg 2520 ggagacattg ctgccaggga cctgatttgt gcccagaagt tcaatggact gacagtgctg 2580 cctccactgc tgacagatga gatgattgcc caatacacct ctgccctgct ggctggcacc 2640 atcacctctg gctggacctt tggagcagga gcagccctcc aaatcccatt tgctatgcag 2700 atggcttaca ggttcaatgg cattggagtg acccagaatg tgctctatga gaaccagaaa 2760 ctgattgcca accagttcaa ctctgccatt ggcaagattc aggactccct gtccagcaca 2820 gcctctgccc tgggcaaact ccaagatgtg gtgaaccaga atgcccaggc tctgaacacc 2880 ctggtgaagc aactttccag caactttgga gccatctcct ctgtgctgaa tgacatcctg 2940 agcagactgg acaaggtgga ggctgaggtc cagattgaca gactgattac aggcagactc 3000 caatccctcc aaacctatgt gacccaacaa cttatcaggg ctgctgagat tagggcatct 3060 gccaacctgg ctgccaccaa gatgagtgag tgtgtgctgg gacaaagcaa gagggtggac 3120 ttctgtggca agggctacca cctgatgagt tttccacagt ctgcccctca tggagtggtg 3180 ttcctgcatg tgacctatgt gcctgcccag gagaagaact tcaccacagc ccctgccatc 3240 tgccatgatg gcaaggctca ctttccaagg gagggagtgt ttgtgagcaa tggcacccac 3300 tggtttgtga cccagaggaa cttctatgaa ccacagatta tcaccacaga caacaccttt 3360 gtgtctggca actgtgatgt ggtgattggc attgtgaaca acacagtcta tgacccactc 3420 caacctgaac tggactcctt caaggaggaa ctggacaaat acttcaagaa ccacaccagc 3480 cctgatgtgg acctgggaga catctctggc atcaatgcct ctgtggtgaa catccagaag 3540 gagattgaca gactgaatga ggtggctaag aacctgaatg agtccctgat tgacctccaa 3600 gaactgggca aatatgaaca atacatcaag tggccatga 3639 <210> 13 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 13 cggtggcggt gggtcgaggt ctgtggcaag ccagag 36 <210> 14 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 14 gacccaccgc caccggtctg ggtctggtag gagg 34 <210> 15 <211> 57 <212> DNA <213> Artificial Sequence <220> <223> linker <400> 15 accggtggcg gtgggtcggg cggtggtggg tcgggtggcg gcggttccag gtctgtg 57 <210> 16 <211> 3669 <212> DNA <213> Artificial Sequence <220> <223> spike protein coding sequence in #4 vector <400> 16 atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc 60 aggacccaac ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac 120 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc 180 aatgtgacct ggttccatgc catccatgtg tctggcacca atggcaccaa gaggtttgac 240 aaccctgtgc tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc 300 atcaggggct ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg 360 aacaatgcca ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc 420 ctgggagtct actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac 480 tcctctgcca acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag 540 ggcaagcagg gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac 600 ttcaagattt acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc 660 tctgccttgg aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc 720 ctgctggctc tgcacaggtc ctacctgaca cctggagact cctcctctgg ctggacagca 780 ggagcagcag cctactatgt gggctacctc caaccaagga ccttcctgct gaaatacaat 840 gagaatggca ccatcacaga tgctgtggac tgtgccctgg acccactgtc tgagaccaag 900 tgtaccctga aatccttcac agtggagaag ggcatctacc agaccagcaa cttcagggtc 960 caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag 1020 gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac 1080 tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat 1140 ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt 1200 gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac 1260 tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac 1320 ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac 1380 ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt 1440 aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc 1500 aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc 1560 cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac 1620 ttcaacttca atggactgac aggcacagga gtgctgacag agagcaacaa gaagttcctg 1680 ccattccaac agtttggcag ggacattgct gacaccacag atgctgtgag ggacccacag 1740 accttggaga ttctggacat cacaccatgt tcctttggag gagtgtctgt gattacacct 1800 ggcaccaaca ccagcaacca ggtggctgtg ctctaccagg atgtgaactg tactgaggtg 1860 cctgtggcta tccatgctga ccaacttaca ccaacctgga gggtctacag cacaggcagc 1920 aatgtgttcc agaccagggc tggctgtctg attggagcag agcatgtgaa caactcctat 1980 gagtgtgaca tcccaattgg agcaggcatc tgtgcctcct accagaccca gaccggtggc 2040 ggtgggtcgg gcggtggtgg gtcgggtggc ggcggttcca ggtctgtggc aagccagagc 2100 atcattgcct acacaatgag tctgggagca gagaactctg tggcttacag caacaacagc 2160 attgccatcc caaccaactt caccatctct gtgaccacag agattctgcc tgtgagtatg 2220 accaagacct ctgtggactg tacaatgtat atctgtggag acagcacaga gtgtagcaac 2280 ctgctgctcc aatatggctc cttctgtacc caacttaaca gggctctgac aggcattgct 2340 gtggaacagg acaagaacac ccaggaggtg tttgcccagg tgaagcagat ttacaagaca 2400 cctccaatca aggactttgg aggcttcaac ttcagccaga ttctgcctga cccaagcaag 2460 ccaagcaaga ggtccttcat tgaggacctg ctgttcaaca aggtgaccct ggctgatgct 2520 ggcttcatca agcaatatgg agactgtctg ggagacattg ctgccaggga cctgatttgt 2580 gcccagaagt tcaatggact gacagtgctg cctccactgc tgacagatga gatgattgcc 2640 caatacacct ctgccctgct ggctggcacc atcacctctg gctggacctt tggagcagga 2700 gcagccctcc aaatcccatt tgctatgcag atggcttaca ggttcaatgg cattggagtg 2760 acccagaatg tgctctatga gaaccagaaa ctgattgcca accagttcaa ctctgccatt 2820 ggcaagattc aggactccct gtccagcaca gcctctgccc tgggcaaact ccaagatgtg 2880 gtgaaccaga atgcccaggc tctgaacacc ctggtgaagc aactttccag caactttgga 2940 gccatctcct ctgtgctgaa tgacatcctg agcagactgg acaaggtgga ggctgaggtc 3000 cagattgaca gactgattac aggcagactc caatccctcc aaacctatgt gacccaacaa 3060 cttatcaggg ctgctgagat tagggcatct gccaacctgg ctgccaccaa gatgagtgag 3120 tgtgtgctgg gacaaagcaa gagggtggac ttctgtggca agggctacca cctgatgagt 3180 tttccacagt ctgcccctca tggagtggtg ttcctgcatg tgacctatgt gcctgcccag 3240 gagaagaact tcaccacagc ccctgccatc tgccatgatg gcaaggctca ctttccaagg 3300 gagggagtgt ttgtgagcaa tggcacccac tggtttgtga cccagaggaa cttctatgaa 3360 ccacagatta tcaccacaga caacaccttt gtgtctggca actgtgatgt ggtgattggc 3420 attgtgaaca acacagtcta tgacccactc caacctgaac tggactcctt caaggaggaa 3480 ctggacaaat acttcaagaa ccacaccagc cctgatgtgg acctgggaga catctctggc 3540 atcaatgcct ctgtggtgaa catccagaag gagattgaca gactgaatga ggtggctaag 3600 aacctgaatg agtccctgat tgacctccaa gaactgggca aatatgaaca atacatcaag 3660 tggccatga 3669 <210> 17 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 17 gggcggtggt gggtcgggtg gcggcggttc caggtctgtg gcaagccaga g 51 <210> 18 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 18 gacccaccac cgcccgaccc accgccaccg gtctgggtct ggtaggagg 49 <210> 19 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> #3 linker <400> 19 Thr Gly Gly Gly Gly Ser Arg 1 5 <210> 20 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> #4 linker <400> 20 Thr Gly Gly Gly Gly Ser Arg Arg Arg 1 5 <210> 21 <211> 31091 <212> DNA <213> Artificial Sequence <220> <223> Adk35F <400> 21 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct 960 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 1020 agacaccggg accgatccag cctccgattt aaattgatca taatcagcca taccacattt 1080 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 1140 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 1200 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 1260 tccaaactca tcaatgtatc ttaacgcgga tctgggcgtg gttaagggtg ggaaagaata 1320 tataaggtgg gggtcttatg tagttttgta tctgttttgc agcagccgcc gccgccatga 1380 gcaccaactc gtttgatgga agcattgtga gctcatattt gacaacgcgc atgcccccat 1440 gggccggggt gcgtcagaat gtgatgggct ccagcattga tggtcgcccc gtcctgcccg 1500 caaactctac taccttgacc tacgagaccg tgtctggaac gccgttggag actgcagcct 1560 ccgccgccgc ttcagccgct gcagccaccg cccgcgggat tgtgactgac tttgctttcc 1620 tgagcccgct tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac aagttgacgg 1680 ctcttttggc acaattggat tctttgaccc gggaacttaa tgtcgtttct cagcagctgt 1740 tggatctgcg ccagcaggtt tctgccctga aggcttcctc ccctcccaat gcggtttaaa 1800 acataaataa aaaaccagac tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt 1860 atttaggggt tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc 1920 tgtgtatttt ttccaggacg tggtaaaggt gactctggat gttcagatac atgggcataa 1980 gcccgtctct ggggtggagg tagcaccact gcagagcttc atgctgcggg gtggtgttgt 2040 agatgatcca gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca 2100 agctgattgc caggggcagg cccttggtgt aagtgtttac aaagcggtta agctgggatg 2160 ggtgcatacg tggggatatg agatgcatct tggactgtat ttttaggttg gctatgttcc 2220 cagccatatc cctccgggga ttcatgttgt gcagaaccac cagcacagtg tatccggtgc 2280 acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg gagacgccct 2340 tgtgacctcc aagattttcc atgcattcgt ccataatgat ggcaatgggc ccacgggcgg 2400 cggcctgggc gaagatattt ctgggatcac taacgtcata gttgtgttcc aggatgagat 2460 cgtcataggc catttttaca aagcgcgggc ggagggtgcc agactgcggt ataatggttc 2520 catccggccc aggggcgtag ttaccctcac agatttgcat ttcccacgct ttgagttcag 2580 atggggggat catgtctacc tgcggggcga tgaagaaaac ggtttccggg gtaggggaga 2640 tcagctggga agaaagcagg ttcctgagca gctgcgactt accgcagccg gtgggcccgt 2700 aaatcacacc tattaccggc tgcaactggt agttaagaga gctgcagctg ccgtcatccc 2760 tgagcagggg ggccacttcg ttaagcatgt ccctgactcg catgttttcc ctgaccaaat 2820 ccgccagaag gcgctcgccg cccagcgata gcagttcttg caaggaagca aagtttttca 2880 acggtttgag accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc 2940 ggtcccacag ctcggtcacc tgctctacgg catctcgatc cagcatatct cctcgtttcg 3000 cgggttgggg cggctttcgc tgtacggcag tagtcggtgc tcgtccagac gggccagggt 3060 catgtctttc cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg 3120 cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg 3180 ctgccggtct tcgccctgcg cgtcggccag gtagcatttg accatggtgt catagtccag 3240 cccctccgcg gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg 3300 gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga aataccgatt ccggggagta 3360 ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc acgagccagg tgagctctgg 3420 ccgttcgggg tcaaaaacca ggtttccccc atgctttttg atgcgtttct tacctctggt 3480 ttccatgagc cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga 3540 cttgagaggc ctgtcctcga gcggtgttcc gcggtcctcc tcgtatagaa actcggacca 3600 ctctgagaca aaggctcgcg tccaggccag cacgaaggag gctaagtggg aggggtagcg 3660 gtcgttgtcc actagggggt ccactcgctc cagggtgtga agacacatgt cgccctcttc 3720 ggcatcaagg aaggtgattg gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg 3780 ggggctataa aagggggtgg gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc 3840 gagggccagc tgttggggtg agtactccct ctgaaaagcg ggcatgactt ctgcgctaag 3900 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt 3960 gagggtggcc gcatccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc 4020 aaacgacccg tagagggcgt tggacagcaa cttggcgatg gagcgcaggg tttggttttt 4080 gtcgcgatcg gcgcgctcct tggccgcgat gtttagctgc acgtattcgc gcgcaacgca 4140 ccgccattcg ggaaagacgg tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg 4200 gttgtgcagg gtgacaaggt caacgctggt ggctacctct ccgcgtaggc gctcgttggt 4260 ccagcagagg cggccgccct tgcgcgagca gaatggcggt agggggtcta gctgcgtctc 4320 gtccgggggg tctgcgtcca cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc 4380 tatcttgcat ccttgcaagt ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc 4440 gtatgggttg agtgggggac cccatggcat ggggtgggtg agcgcggagg cgtacatgcc 4500 gcaaatgtcg taaacgtaga ggggctctct gagtattcca agatatgtag ggtagcatct 4560 tccaccgcgg atgctggcgc gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc 4620 gggaccgagg ttgctacggg cgggctgctc tgctcggaag actatctgcc tgaagatggc 4680 atgtgagttg gatgatatgg ttggacgctg gaagacgttg aagctggcgt ctgtgagacc 4740 taccgcgtca cgcacgaagg aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt 4800 gacctgcacg tctagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg 4860 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtactc 4920 ttggatcgga aacccgtcgg cctccgaacg gtaagagcct agcatgtaga actggttgac 4980 ggcctggtag gcgcagcatc ccttttctac gggtagcgcg tatgcctgcg cggccttccg 5040 gagcgaggtg tgggtgagcg caaaggtgtc cctgaccatg actttgaggt actggtattt 5100 gaagtcagtg tcgtcgcatc cgccctgctc ccagagcaaa aagtccgtgc gctttttgga 5160 acgcggattt ggcagggcga aggtgacatc gttgaagagt atctttcccg cgcgaggcat 5220 aaagttgcgt gtgatgcgga agggtcccgg cacctcggaa cggttgttaa ttacctgggc 5280 ggcgagcacg atctcgtcaa agccgttgat gttgtggccc acaatgtaaa gttccaagaa 5340 gcgcgggatg cccttgatgg aaggcaattt tttaagttcc tcgtaggtga gctcttcagg 5400 ggagctgagc ccgtgctctg aaagggccca gtctgcaaga tgagggttgg aagcgacgaa 5460 tgagctccac aggtcacggg ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg 5520 gcgacctatg gccatttttt ctggggtgat gcagtagaag gtaagcgggt cttgttccca 5580 gcggtcccat ccaaggttcg cggctaggtc tcgcgcggca gtcactagag gctcatctcc 5640 gccgaacttc atgaccagca tgaagggcac gagctgcttc ccaaaggccc ccatccaagt 5700 ataggtctct acatcgtagg tgacaaagag acgctcggtg cgaggatgcg agccgatcgg 5760 gaagaactgg atctcccgcc accaattgga ggagtggcta ttgatgtggt gaaagtagaa 5820 gtccctgcga cgggccgaac actcgtgctg gcttttgtaa aaacgtgcgc agtactggca 5880 gcggtgcacg ggctgtacat cctgcacgag gttgacctga cgaccgcgca caaggaagca 5940 gagtgggaat ttgagcccct cgcctggcgg gtttggctgg tggtcttcta cttcggctgc 6000 ttgtccttga ccgtctggct gctcgagggg agttacggtg gatcggacca ccacgccgcg 6060 cgagcccaaa gtccagatgt ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag 6120 atgggagctg tccatggtct ggagctcccg cggcgtcagg tcaggcggga gctcctgcag 6180 gtttacctcg catagacggg tcagggcgcg ggctagatcc aggtgatacc taatttccag 6240 gggctggttg gtggcggcgt cgatggcttg caagaggccg catccccgcg gcgcgactac 6300 ggtaccgcgc ggcgggcggt gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg 6360 tgacgcgggc gagcccccgg aggtaggggg ggctccggac ccgccgggag agggggcagg 6420 ggcacgtcgg cgccgcgcgc gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac 6480 gcgacgacgc ggcggttgat ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg 6540 gtgagcttga acctgaaaga gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc 6600 tggcgcaaaa tctcctgcac gtctcctgag ttgtcttgat aggcgatctc ggccatgaac 6660 tgctcgatct cttcctcctg gagatctccg cgtccggctc gctccacggt ggcggcgagg 6720 tcgttggaaa tgcgggccat gagctgcgag aaggcgttga ggcctccctc gttccagacg 6780 cggctgtaga ccacgccccc ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg 6840 agctccacgt gccgggcgaa gacggcgtag tttcgcaggc gctgaaagag gtagttgagg 6900 gtggtggcgg tgtgttctgc cacgaagaag tacataaccc agcgtcgcaa cgtggattcg 6960 ttgatatccc ccaaggcctc aaggcgctcc atggcctcgt agaagtccac ggcgaagttg 7020 aaaaactggg agttgcgcgc cgacacggtt aactcctcct ccagaagacg gatgagctcg 7080 gcgacagtgt cgcgcacctc gcgctcaaag gctacagggg cctcttcttc ttcttcaatc 7140 tcctcttcca taagggcctc cccttcttct tcttctggcg gcggtggggg aggggggaca 7200 cggcggcgac gacggcgcac cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg 7260 cgacggcgca tggtctcggt gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg 7320 ccgcccgtca tgtcccggtt atgggttggc ggggggctgc catgcggcag ggatacggcg 7380 ctaacgatgc atctcaacaa ttgttgtgta ggtactccgc cgccgaggga cctgagcgag 7440 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa 7500 ggtaggctga gcaccgtggc gggcggcagc gggcggcggt cggggttgtt tctggcggag 7560 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgacagaagc 7620 accatgtcct tgggtccggc ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg 7680 ttttgacatc ggcgcaggtc tttgtagtag tcttgcatga gcctttctac cggcacttct 7740 tcttctcctt cctcttgtcc tgcatctctt gcatctatcg ctgcggcggc ggcggagttt 7800 ggccgtaggt ggcgccctct tcctcccatg cgtgtgaccc cgaagcccct catcggctga 7860 agcagggcta ggtcggcgac aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg 7920 gtagactgga agtcatccat gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa 7980 gtgcagttgg ccataacgga ccagttaacg gtctggtgac ccggctgcga gagctcggtg 8040 tacctgagac gcgagtaagc cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg 8100 tactggtatc ccaccaaaaa gtgcggcggc ggctggcggt agaggggcca gcgtagggtg 8160 gccggggctc cgggggcgag atcttccaac ataaggcgat gatatccgta gatgtacctg 8220 gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc 8280 cagatgttgc gcagcggcaa aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc 8340 gcgcaatcgt tgacgctcta gcgtgcaaaa ggagagcctg taagcgggca ctcttccgtg 8400 gtctggtgga taaattcgca agggtatcat ggcggacgac cggggttcga gccccgtatc 8460 cggccgtccg ccgtgatcca tgcggttacc gcccgcgtgt cgaacccagg tgtgcgacgt 8520 cagacaacgg gggagtgctc cttttggctt ccttccaggc gcggcggctg ctgcgctagc 8580 ttttttggcc actggccgcg cgcagcgtaa gcggttaggc tggaaagcga aagcattaag 8640 tggctcgctc cctgtagccg gagggttatt ttccaagggt tgagtcgcgg gacccccggt 8700 tcgagtctcg gaccggccgg actgcggcga acgggggttt gcctccccgt catgcaagac 8760 cccgcttgca aattcctccg gaaacaggga cgagcccctt ttttgctttt cccagatgca 8820 tccggtgctg cggcagatgc gcccccctcc tcagcagcgg caagagcaag agcagcggca 8880 gacatgcagg gcaccctccc ctcctcctac cgcgtcagga ggggcgacat ccgcggttga 8940 cgcggcagca gatggtgatt acgaaccccc gcggcgccgg gcccggcact acctggactt 9000 ggaggagggc gagggcctgg cgcggctagg agcgccctct cctgagcggc acccaagggt 9060 gcagctgaag cgtgatacgc gtgaggcgta cgtgccgcgg cagaacctgt ttcgcgaccg 9120 cgagggagag gagcccgagg agatgcggga tcgaaagttc cacgcagggc gcgagctgcg 9180 gcatggcctg aatcgcgagc ggttgctgcg cgaggaggac tttgagcccg acgcgcgaac 9240 cgggattagt cccgcgcgcg cacacgtggc ggccgccgac ctggtaaccg catacgagca 9300 gacggtgaac caggagatta actttcaaaa aagctttaac aaccacgtgc gtacgcttgt 9360 ggcgcgcgag gaggtggcta taggactgat gcatctgtgg gactttgtaa gcgcgctgga 9420 gcaaaaccca aatagcaagc cgctcatggc gcagctgttc cttatagtgc agcacagcag 9480 ggacaacgag gcattcaggg atgcgctgct aaacatagta gagcccgagg gccgctggct 9540 gctcgatttg ataaacatcc tgcagagcat agtggtgcag gagcgcagct tgagcctggc 9600 tgacaaggtg gccgccatca actattccat gcttagcctg ggcaagtttt acgcccgcaa 9660 gatataccat accccttacg ttcccataga caaggaggta aagatcgagg ggttctacat 9720 gcgcatggcg ctgaaggtgc ttaccttgag cgacgacctg ggcgtttatc gcaacgagcg 9780 catccacaag gccgtgagcg tgagccggcg gcgcgagctc agcgaccgcg agctgatgca 9840 cagcctgcaa agggccctgg ctggcacggg cagcggcgat agagaggccg agtcctactt 9900 tgacgcgggc gctgacctgc gctgggcccc aagccgacgc gccctggagg cagctggggc 9960 cggacctggg ctggcggtgg cacccgcgcg cgctggcaac gtcggcggcg tggaggaata 10020 tgacgaggac gatgagtacg agccagagga cggcgagtac taagcggtga tgtttctgat 10080 cagatgatgc aagacgcaac ggacccggcg gtgcgggcgg cgctgcagag ccagccgtcc 10140 ggccttaact ccacggacga ctggcgccag gtcatggacc gcatcatgtc gctgactgcg 10200 cgcaatcctg acgcgttccg gcagcagccg caggccaacc ggctctccgc aattctggaa 10260 gcggtggtcc cggcgcgcgc aaaccccacg cacgagaagg tgctggcgat cgtaaacgcg 10320 ctggccgaaa acagggccat ccggcccgac gaggccggcc tggtctacga cgcgctgctt 10380 cagcgcgtgg ctcgttacaa cagcggcaac gtgcagacca acctggaccg gctggtgggg 10440 gatgtgcgcg aggccgtggc gcagcgtgag cgcgcgcagc agcagggcaa cctgggctcc 10500 atggttgcac taaacgcctt cctgagtaca cagcccgcca acgtgccgcg gggacaggag 10560 gactacacca actttgtgag cgcactgcgg ctaatggtga ctgagacacc gcaaagtgag 10620 gtgtaccagt ctgggccaga ctattttttc cagaccagta gacaaggcct gcagaccgta 10680 aacctgagcc aggctttcaa aaacttgcag gggctgtggg gggtgcgggc tcccacaggc 10740 gaccgcgcga ccgtgtctag cttgctgacg cccaactcgc gcctgttgct gctgctaata 10800 gcgcccttca cggacagtgg cagcgtgtcc cgggacacat acctaggtca cttgctgaca 10860 ctgtaccgcg aggccatagg tcaggcgcat gtggacgagc atactttcca ggagattaca 10920 agtgtcagcc gcgcgctggg gcaggaggac acgggcagcc tggaggcaac cctaaactac 10980 ctgctgacca accggcggca gaagatcccc tcgttgcaca gtttaaacag cgaggaggag 11040 cgcattttgc gctacgtgca gcagagcgtg agccttaacc tgatgcgcga cggggtaacg 11100 cccagcgtgg cgctggacat gaccgcgcgc aacatggaac cgggcatgta tgcctcaaac 11160 cggccgttta tcaaccgcct aatggactac ttgcatcgcg cggccgccgt gaaccccgag 11220 tatttcacca atgccatctt gaacccgcac tggctaccgc cccctggttt ctacaccggg 11280 ggattcgagg tgcccgaggg taacgatgga ttcctctggg acgacataga cgacagcgtg 11340 ttttccccgc aaccgcagac cctgctagag ttgcaacagc gcgagcaggc agaggcggcg 11400 ctgcgaaagg aaagcttccg caggccaagc agcttgtccg atctaggcgc tgcggccccg 11460 cggtcagatg ctagtagccc atttccaagc ttgatagggt ctcttaccag cactcgcacc 11520 acccgcccgc gcctgctggg cgaggaggag tacctaaaca actcgctgct gcagccgcag 11580 cgcgaaaaaa acctgcctcc ggcatttccc aacaacggga tagagagcct agtggacaag 11640 atgagtagat ggaagacgta cgcgcaggag cacagggacg tgccaggccc gcgcccgccc 11700 acccgtcgtc aaaggcacga ccgtcagcgg ggtctggtgt gggaggacga tgactcggca 11760 gacgacagca gcgtcctgga tttgggaggg agtggcaacc cgtttgcgca ccttcgcccc 11820 aggctgggga gaatgtttta aaaaaaaaaa agcatgatgc aaaataaaaa actcaccaag 11880 gccatggcac cgagcgttgg ttttcttgta ttccccttag tatgcggcgc gcggcgatgt 11940 atgaggaagg tcctcctccc tcctacgaga gtgtggtgag cgcggcgcca gtggcggcgg 12000 cgctgggttc tcccttcgat gctcccctgg acccgccgtt tgtgcctccg cggtacctgc 12060 ggcctaccgg ggggagaaac agcatccgtt actctgagtt ggcaccccta ttcgacacca 12120 cccgtgtgta cctggtggac aacaagtcaa cggatgtggc atccctgaac taccagaacg 12180 accacagcaa ctttctgacc acggtcattc aaaacaatga ctacagcccg ggggaggcaa 12240 gcacacagac catcaatctt gacgaccggt cgcactgggg cggcgacctg aaaaccatcc 12300 tgcataccaa catgccaaat gtgaacgagt tcatgtttac caataagttt aaggcgcggg 12360 tgatggtgtc gcgcttgcct actaaggaca atcaggtgga gctgaaatac gagtgggtgg 12420 agttcacgct gcccgagggc aactactccg agaccatgac catagacctt atgaacaacg 12480 cgatcgtgga gcactacttg aaagtgggca gacagaacgg ggttctggaa agcgacatcg 12540 gggtaaagtt tgacacccgc aacttcagac tggggtttga ccccgtcact ggtcttgtca 12600 tgcctggggt atatacaaac gaagccttcc atccagacat cattttgctg ccaggatgcg 12660 gggtggactt cacccacagc cgcctgagca acttgttggg catccgcaag cggcaaccct 12720 tccaggaggg ctttaggatc acctacgatg atctggaggg tggtaacatt cccgcactgt 12780 tggatgtgga cgcctaccag gcgagcttga aagatgacac cgaacagggc gggggtggcg 12840 caggcggcag caacagcagt ggcagcggcg cggaagagaa ctccaacgcg gcagccgcgg 12900 caatgcagcc ggtggaggac atgaacgatc atgccattcg cggcgacacc tttgccacac 12960 gggctgagga gaagcgcgct gaggccgaag cagcggccga agctgccgcc cccgctgcgc 13020 aacccgaggt cgagaagcct cagaagaaac cggtgatcaa acccctgaca gaggacagca 13080 agaaacgcag ttacaaccta ataagcaatg acagcacctt cacccagtac cgcagctggt 13140 accttgcata caactacggc gaccctcaga ccggaatccg ctcatggacc ctgctttgca 13200 ctcctgacgt aacctgcggc tcggagcagg tctactggtc gttgccagac atgatgcaag 13260 accccgtgac cttccgctcc acgcgccaga tcagcaactt tccggtggtg ggcgccgagc 13320 tgttgcccgt gcactccaag agcttctaca acgaccaggc cgtctactcc caactcatcc 13380 gccagtttac ctctctgacc cacgtgttca atcgctttcc cgagaaccag attttggcgc 13440 gcccgccagc ccccaccatc accaccgtca gtgaaaacgt tcctgctctc acagatcacg 13500 ggacgctacc gctgcgcaac agcatcggag gagtccagcg agtgaccatt actgacgcca 13560 gacgccgcac ctgcccctac gtttacaagg ccctgggcat agtctcgccg cgcgtcctat 13620 cgagccgcac tttttgagca agcatgtcca tccttatatc gcccagcaat aacacaggct 13680 ggggcctgcg cttcccaagc aagatgtttg gcggggccaa gaagcgctcc gaccaacacc 13740 cagtgcgcgt gcgcgggcac taccgcgcgc cctggggcgc gcacaaacgc ggccgcactg 13800 ggcgcaccac cgtcgatgac gccatcgacg cggtggtgga ggaggcgcgc aactacacgc 13860 ccacgccgcc accagtgtcc acagtggacg cggccattca gaccgtggtg cgcggagccc 13920 ggcgctatgc taaaatgaag agacggcgga ggcgcgtagc acgtcgccac cgccgccgac 13980 ccggcactgc cgcccaacgc gcggcggcgg ccctgcttaa ccgcgcacgt cgcaccggcc 14040 gacgggcggc catgcgggcc gctcgaaggc tggccgcggg tattgtcact gtgcccccca 14100 ggtccaggcg acgagcggcc gccgcagcag ccgcggccat tagtgctatg actcagggtc 14160 gcaggggcaa cgtgtattgg gtgcgcgact cggttagcgg cctgcgcgtg cccgtgcgca 14220 cccgcccccc gcgcaactag attgcaagaa aaaactactt agactcgtac tgttgtatgt 14280 atccagcggc ggcggcgcgc aacgaagcta tgtccaagcg caaaatcaaa gaagagatgc 14340 tccaggtcat cgcgccggag atctatggcc ccccgaagaa ggaagagcag gattacaagc 14400 cccgaaagct aaagcgggtc aaaaagaaaa agaaagatga tgatgatgaa cttgacgacg 14460 aggtggaact gctgcacgct accgcgccca ggcgacgggt acagtggaaa ggtcgacgcg 14520 taaaacgtgt tttgcgaccc ggcaccaccg tagtctttac gcccggtgag cgctccaccc 14580 gcacctacaa gcgcgtgtat gatgaggtgt acggcgacga ggacctgctt gagcaggcca 14640 acgagcgcct cggggagttt gcctacggaa agcggcataa ggacatgctg gcgttgccgc 14700 tggacgaggg caacccaaca cctagcctaa agcccgtaac actgcagcag gtgctgcccg 14760 cgcttgcacc gtccgaagaa aagcgcggcc taaagcgcga gtctggtgac ttggcaccca 14820 ccgtgcagct gatggtaccc aagcgccagc gactggaaga tgtcttggaa aaaatgaccg 14880 tggaacctgg gctggagccc gaggtccgcg tgcggccaat caagcaggtg gcgccgggac 14940 tgggcgtgca gaccgtggac gttcagatac ccactaccag tagcaccagt attgccaccg 15000 ccacagaggg catggagaca caaacgtccc cggttgcctc agcggtggcg gatgccgcgg 15060 tgcaggcggt cgctgcggcc gcgtccaaga cctctacgga ggtgcaaacg gacccgtgga 15120 tgtttcgcgt ttcagccccc cggcgcccgc gccgttcgag gaagtacggc gccgccagcg 15180 cgctactgcc cgaatatgcc ctacatcctt ccattgcgcc tacccccggc tatcgtggct 15240 acacctaccg ccccagaaga cgagcaacta cccgacgccg aaccaccact ggaacccgcc 15300 gccgccgtcg ccgtcgccag cccgtgctgg ccccgatttc cgtgcgcagg gtggctcgcg 15360 aaggaggcag gaccctggtg ctgccaacag cgcgctacca ccccagcatc gtttaaaagc 15420 cggtctttgt ggttcttgca gatatggccc tcacctgccg cctccgtttc ccggtgccgg 15480 gattccgagg aagaatgcac cgtaggaggg gcatggccgg ccacggcctg acgggcggca 15540 tgcgtcgtgc gcaccaccgg cggcggcgcg cgtcgcaccg tcgcatgcgc ggcggtatcc 15600 tgcccctcct tattccactg atcgccgcgg cgattggcgc cgtgcccgga attgcatccg 15660 tggccttgca ggcgcagaga cactgattaa aaacaagttg catgtggaaa aatcaaaata 15720 aaaagtctgg actctcacgc tcgcttggtc ctgtaactat tttgtagaat ggaagacatc 15780 aactttgcgt ctctggcccc gcgacacggc tcgcgcccgt tcatgggaaa ctggcaagat 15840 atcggcacca gcaatatgag cggtggcgcc ttcagctggg gctcgctgtg gagcggcatt 15900 aaaaatttcg gttccaccgt taagaactat ggcagcaagg cctggaacag cagcacaggc 15960 cagatgctga gggataagtt gaaagagcaa aatttccaac aaaaggtggt agatggcctg 16020 gcctctggca ttagcggggt ggtggacctg gccaaccagg cagtgcaaaa taagattaac 16080 agtaagcttg atccccgccc tcccgtagag gagcctccac cggccgtgga gacagtgtct 16140 ccagaggggc gtggcgaaaa gcgtccgcgc cccgacaggg aagaaactct ggtgacgcaa 16200 atagacgagc ctccctcgta cgaggaggca ctaaagcaag gcctgcccac cacccgtccc 16260 atcgcgccca tggctaccgg agtgctgggc cagcacacac ccgtaacgct ggacctgcct 16320 ccccccgccg acacccagca gaaacctgtg ctgccaggcc cgaccgccgt tgttgtaacc 16380 cgtcctagcc gcgcgtccct gcgccgcgcc gccagcggtc cgcgatcgtt gcggcccgta 16440 gccagtggca actggcaaag cacactgaac agcatcgtgg gtctgggggt gcaatccctg 16500 aagcgccgac gatgcttctg atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg 16560 tcgccgccag aggagctgct gagccgccgc gcgcccgctt tccaagatgg ctaccccttc 16620 gatgatgccg cagtggtctt acatgcacat ctcgggccag gacgcctcgg agtacctgag 16680 ccccgggctg gtgcagtttg cccgcgccac cgagacgtac ttcagcctga ataacaagtt 16740 tagaaacccc acggtggcgc ctacgcacga cgtgaccaca gaccggtccc agcgtttgac 16800 gctgcggttc atccctgtgg accgtgagga tactgcgtac tcgtacaagg cgcggttcac 16860 cctagctgtg ggtgataacc gtgtgctgga catggcttcc acgtactttg acatccgcgg 16920 cgtgctggac aggggcccta cttttaagcc ctactctggc actgcctaca acgccctggc 16980 tcccaagggt gccccaaatc cttgcgaatg ggatgaagct gctactgctc ttgaaataaa 17040 cctagaagaa gaggacgatg acaacgaaga cgaagtagac gagcaagctg agcagcaaaa 17100 aactcacgta tttgggcagg cgccttattc tggtataaat attacaaagg agggtattca 17160 aataggtgtc gaaggtcaaa cacctaaata tgccgataaa acatttcaac ctgaacctca 17220 aataggagaa tctcagtggt acgaaacaga aattaatcat gcagctggga gagtcctaaa 17280 aaagactacc ccaatgaaac catgttacgg ttcatatgca aaacccacaa atgaaaatgg 17340 agggcaaggc attcttgtaa agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca 17400 atttttctca actactgagg cagccgcagg caatggtgat aacttgactc ctaaagtggt 17460 attgtacagt gaagatgtag atatagaaac cccagacact catatttctt acatgcccac 17520 tattaaggaa ggtaactcac gagaactaat gggccaacaa tctatgccca acaggcctaa 17580 ttacattgct tttagggaca attttattgg tctaatgtat tacaacagca cgggtaatat 17640 gggtgttctg gcgggccaag catcgcagtt gaatgctgtt gtagatttgc aagacagaaa 17700 cacagagctt tcataccagc ttttgcttga ttccattggt gatagaacca ggtacttttc 17760 tatgtggaat caggctgttg acagctatga tccagatgtt agaattattg aaaatcatgg 17820 aactgaagat gaacttccaa attactgctt tccactggga ggtgtgatta atacagagac 17880 tcttaccaag gtaaaaccta aaacaggtca ggaaaatgga tgggaaaaag atgctacaga 17940 attttcagat aaaaatgaaa taagagttgg aaataatttt gccatggaaa tcaatctaaa 18000 tgccaacctg tggagaaatt tcctgtactc caacatagcg ctgtatttgc ccgacaagct 18060 aaagtacagt ccttccaacg taaaaatttc tgataaccca aacacctacg actacatgaa 18120 caagcgagtg gtggctcccg ggctagtgga ctgctacatt aaccttggag cacgctggtc 18180 ccttgactat atggacaacg tcaacccatt taaccaccac cgcaatgctg gcctgcgcta 18240 ccgctcaatg ttgctgggca atggtcgcta tgtgcccttc cacatccagg tgcctcagaa 18300 gttctttgcc attaaaaacc tccttctcct gccgggctca tacacctacg agtggaactt 18360 caggaaggat gttaacatgg ttctgcagag ctccctagga aatgacctaa gggttgacgg 18420 agccagcatt aagtttgata gcatttgcct ttacgccacc ttcttcccca tggcccacaa 18480 caccgcctcc acgcttgagg ccatgcttag aaacgacacc aacgaccagt cctttaacga 18540 ctatctctcc gccgccaaca tgctctaccc tatacccgcc aacgctacca acgtgcccat 18600 atccatcccc tcccgcaact gggcggcttt ccgcggctgg gccttcacgc gccttaagac 18660 taaggaaacc ccatcactgg gctcgggcta cgacccttat tacacctact ctggctctat 18720 accctaccta gatggaacct tttacctcaa ccacaccttt aagaaggtgg ccattacctt 18780 tgactcttct gtcagctggc ctggcaatga ccgcctgctt acccccaacg agtttgaaat 18840 taagcgctca gttgacgggg agggttacaa cgttgcccag tgtaacatga ccaaagactg 18900 gttcctggta caaatgctag ctaactataa cattggctac cagggcttct atatcccaga 18960 gagctacaag gaccgcatgt actccttctt tagaaacttc cagcccatga gccgtcaggt 19020 ggtggatgat actaaataca aggactacca acaggtgggc atcctacacc aacacaacaa 19080 ctctggattt gttggctacc ttgcccccac catgcgcgaa ggacaggcct accctgctaa 19140 cttcccctat ccgcttatag gcaagaccgc agttgacagc attacccaga aaaagtttct 19200 ttgcgatcgc accctttggc gcatcccatt ctccagtaac tttatgtcca tgggcgcact 19260 cacagacctg ggccaaaacc ttctctacgc caactccgcc cacgcgctag acatgacttt 19320 tgaggtggat cccatggacg agcccaccct tctttatgtt ttgtttgaag tctttgacgt 19380 ggtccgtgtg caccagccgc accgcggcgt catcgaaacc gtgtacctgc gcacgccctt 19440 ctcggccggc aacgccacaa cataaagaag caagcaacat caacaacagc tgccgccatg 19500 ggctccagtg agcaggaact gaaagccatt gtcaaagatc ttggttgtgg gccatatttt 19560 ttgggcacct atgacaagcg ctttccaggc tttgtttctc cacacaagct cgcctgcgcc 19620 atagtcaata cggccggtcg cgagactggg ggcgtacact ggatggcctt tgcctggaac 19680 ccgcactcaa aaacatgcta cctctttgag ccctttggct tttctgacca gcgactcaag 19740 caggtttacc agtttgagta cgagtcactc ctgcgccgta gcgccattgc ttcttccccc 19800 gaccgctgta taacgctgga aaagtccacc caaagcgtac aggggcccaa ctcggccgcc 19860 tgtggactat tctgctgcat gtttctccac gcctttgcca actggcccca aactcccatg 19920 gatcacaacc ccaccatgaa ccttattacc ggggtaccca actccatgct caacagtccc 19980 caggtacagc ccaccctgcg tcgcaaccag gaacagctct acagcttcct ggagcgccac 20040 tcgccctact tccgcagcca cagtgcgcag attaggagcg ccacttcttt ttgtcacttg 20100 aaaaacatgt aaaaataatg tactagagac actttcaata aaggcaaatg cttttatttg 20160 tacactctcg ggtgattatt tacccccacc cttgccgtct gcgccgttta aaaatcaaag 20220 gggttctgcc gcgcatcgct atgcgccact ggcagggaca cgttgcgata ctggtgttta 20280 gtgctccact taaactcagg cacaaccatc cgcggcagct cggtgaagtt ttcactccac 20340 aggctgcgca ccatcaccaa cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag 20400 ttggggcctc cgccctgcgc gcgcgagttg cgatacacag ggttgcagca ctggaacact 20460 atcagcgccg ggtggtgcac gctggccagc acgctcttgt cggagatcag atccgcgtcc 20520 aggtcctccg cgttgctcag ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag 20580 ggcgcgtgcc caggctttga gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc 20640 ccggtctggg cgttaggata cagcgcctgc ataaaagcct tgatctgctt aaaagccacc 20700 tgagcctttg cgccttcaga gaagaacatg ccgcaagact tgccggaaaa ctgattggcc 20760 ggacaggccg cgtcgtgcac gcagcacctt gcgtcggtgt tggagatctg caccacattt 20820 cggccccacc ggttcttcac gatcttggcc ttgctagact gctccttcag cgcgcgctgc 20880 ccgttttcgc tcgtcacatc catttcaatc acgtgctcct tatttatcat aatgcttccg 20940 tgtagacact taagctcgcc ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc 21000 gtgggctcgt gatgcttgta ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat 21060 cgccccatca tcgtcacaaa ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc 21120 tcctcgttca gccaggtctt gcatacggcc gccagagctt ccacttggtc aggcagtagt 21180 ttgaagttcg cctttagatc gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc 21240 tccatgccct tctcccacgc agacacgatc ggcacactca gcgggttcat caccgtaatt 21300 tcactttccg cttcgctggg ctcttcctct tcctcttgcg tccgcatacc acgcgccact 21360 gggtcgtctt cattcagccg ccgcactgtg cgcttacctc ctttgccatg cttgattagc 21420 accggtgggt tgctgaaacc caccatttgt agcgccacat cttctctttc ttcctcgctg 21480 tccacgatta cctctggtga tggcgggcgc tcgggcttgg gagaagggcg cttctttttc 21540 ttcttgggcg caatggccaa atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc 21600 ggcaccagcg cgtcttgtga tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc 21660 cgcttttttg ggggcgcccg gggaggcggc ggcgacgggg acggggacga cacgtcctcc 21720 atggttgggg gacgtcgcgc cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc 21780 tcttcccgac tggccatttc cttctcctat aggcagaaaa agatcatgga gtcagtcgag 21840 aagaaggaca gcctaaccgc cccctctgag ttcgccacca ccgcctccac cgatgccgcc 21900 aacgcgccta ccaccttccc cgtcgaggca cccccgcttg aggaggagga agtgattatc 21960 gagcaggacc caggttttgt aagcgaagac gacgaggacc gctcagtacc aacagaggat 22020 aaaaagcaag accaggacaa cgcagaggca aacgaggaac aagtcgggcg gggggacgaa 22080 aggcatggcg actacctaga tgtgggagac gacgtgctgt tgaagcatct gcagcgccag 22140 tgcgccatta tctgcgacgc gttgcaagag cgcagcgatg tgcccctcgc catagcggat 22200 gtcagccttg cctacgaacg ccacctattc tcaccgcgcg taccccccaa acgccaagaa 22260 aacggcacat gcgagcccaa cccgcgcctc aacttctacc ccgtatttgc cgtgccagag 22320 gtgcttgcca cctatcacat ctttttccaa aactgcaaga tacccctatc ctgccgtgcc 22380 aaccgcagcc gagcggacaa gcagctggcc ttgcggcagg gcgctgtcat acctgatatc 22440 gcctcgctca acgaagtgcc aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg 22500 gcaaacgctc tgcaacagga aaacagcgaa aatgaaagtc actctggagt gttggtggaa 22560 ctcgagggtg acaacgcgcg cctagccgta ctaaaacgca gcatcgaggt cacccacttt 22620 gcctacccgg cacttaacct accccccaag gtcatgagca cagtcatgag tgagctgatc 22680 gtgcgccgtg cgcagcccct ggagagggat gcaaatttgc aagaacaaac agaggagggc 22740 ctacccgcag ttggcgacga gcagctagcg cgctggcttc aaacgcgcga gcctgccgac 22800 ttggaggagc gacgcaaact aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc 22860 atgcagcggt tctttgctga cccggagatg cagcgcaagc tagaggaaac attgcactac 22920 acctttcgac agggctacgt acgccaggcc tgcaagatct ccaacgtgga gctctgcaac 22980 ctggtctcct accttggaat tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc 23040 acgctcaagg gcgaggcgcg ccgcgactac gtccgcgact gcgtttactt atttctatgc 23100 tacacctggc agacggccat gggcgtttgg cagcagtgct tggaggagtg caacctcaag 23160 gagctgcaga aactgctaaa gcaaaacttg aaggacctat ggacggcctt caacgagcgc 23220 tccgtggccg cgcacctggc ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa 23280 cagggtctgc cagacttcac cagtcaaagc atgttgcaga actttaggaa ctttatccta 23340 gagcgctcag gaatcttgcc cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt 23400 aagtaccgcg aatgccctcc gccgctttgg ggccactgct accttctgca gctagccaac 23460 taccttgcct accactctga cataatggaa gacgtgagcg gtgacggtct actggagtgt 23520 cactgtcgct gcaacctatg caccccgcac cgctccctgg tttgcaattc gcagctgctt 23580 aacgaaagtc aaattatcgg tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc 23640 gcggctccgg ggttgaaact cactccgggg ctgtggacgt cggcttacct tcgcaaattt 23700 gtacctgagg actaccacgc ccacgagatt aggttctacg aagaccaatc ccgcccgcct 23760 aatgcggagc ttaccgcctg cgtcattacc cagggccaca ttcttggcca attgcaagcc 23820 atcaacaaag cccgccaaga gtttctgcta cgaaagggac ggggggttta cttggacccc 23880 cagtccggcg aggagctcaa cccaatcccc ccgccgccgc agccctatca gcagcagccg 23940 cgggcccttg cttcccagga tggcacccaa aaagaagctg cagctgccgc cgccacccac 24000 ggacgaggag gaatactggg acagtcaggc agaggaggtt ttggacgagg aggaggagga 24060 catgatggaa gactgggaga gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga 24120 cgaaacaccg tcaccctcgg tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg 24180 ttccagcatg gctacaacct ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc 24240 caaccgtaga tgggacacca ctggaaccag ggccggtaag tccaagcagc cgccgccgtt 24300 agcccaagag caacaacagc gccaaggcta ccgctcatgg cgcgggcaca agaacgccat 24360 agttgcttgc ttgcaagact gtgggggcaa catctccttc gcccgccgct ttcttctcta 24420 ccatcacggc gtggccttcc cccgtaacat cctgcattac taccgtcatc tctacagccc 24480 atactgcacc ggcggcagcg gcagcaacag cagcggccac acagaagcaa aggcgaccgg 24540 atagcaagac tctgacaaag cccaagaaat ccacagcggc ggcagcagca ggaggaggag 24600 cgctgcgtct ggcgcccaac gaacccgtat cgacccgcga gcttagaaac aggatttttc 24660 ccactctgta tgctatattt caacagagca ggggccaaga acaagagctg aaaataaaaa 24720 acaggtctct gcgatccctc acccgcagct gcctgtatca caaaagcgaa gatcagcttc 24780 ggcgcacgct ggaagacgcg gaggctctct tcagtaaata ctgcgcgctg actcttaagg 24840 actagtttcg cgccctttct caaatttaag cgcgaaaact acgtcatctc cagcggccac 24900 acccggcgcc agcacctgtt gtcagcgcca ttatgagcaa ggaaattccc acgccctaca 24960 tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac tactcaaccc 25020 gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga atacgcgccc 25080 accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt aataacctta 25140 atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc accactgtgg 25200 tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg cagcttgcgg 25260 gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg acaatcagag 25320 ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc cgtccggacg 25380 ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag gcaatcctaa 25440 ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg caatttattg 25500 aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc cactatccgg 25560 atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac gactgaatgt 25620 taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc cgccacaagt 25680 gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat catatcgagg 25740 gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc ctgattcggg 25800 agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt ctcactgtga 25860 tttgcaactg tcctaaccct ggattacatc aagatcctct agttaatgtc aggtcgccta 25920 agtcgattaa ctagagtacc cggggatctt attcccttta actaataaaa aaaaataata 25980 aagcatcact tacttaaaat cagttagcaa atttctgtcc agtttattca gcagcacctc 26040 cttgccctcc tcccagctct ggtattgcag cttcctcctg gctgcaaact ttctccacaa 26100 tctaaatgga atgtcagttt cctcctgttc ctgtccatcc gcacccacta tcttcatgtt 26160 gttgcagatg aagcgcgcaa gaccgtctga agataccttc aaccccgtgt atccatatga 26220 cacggaaacc ggtcctccaa ctgtgccttt tcttactcct ccctttgtat cccccaatgg 26280 gtttcaagag agtccccctg gggtactctc tttgcgccta tccgaacctc tagttacctc 26340 caatggcatg cttgcgctca aaatgggcaa cggcctctct ctggacgagg ccggcaacct 26400 tacctcccaa aatgtaacca ctgtgagccc acctctcaaa aaaaccaagt caaacataaa 26460 cctggaaata tctgcacccc tcacagttac ctcagaagcc ctaactgtgg ctgccgccgc 26520 acctctaatg gtcgcgggca acacactcac catgcaatca caggccccgc taaccgtgca 26580 cgactccaaa cttagcattg ccacccaagg acccctcaca gtgtcagaag gaaagctagc 26640 cctgcaaaca tcaggccccc tcaccaccac cgatagcagt acccttacta tcactgcctc 26700 accccctcta actactgcca ctggtagctt gggcattgac ttgaaagagc ccatttatac 26760 acaaaatgga aaactaggac taaagtacgg ggctcctttg catgtaacag acgacctaaa 26820 cactttgacc gtagcaactg gtccaggtgt gactattaat aatacttcct tgcaaactaa 26880 agttactgga gccttgggtt ttgattcaca aggcaatatg caacttaatg tagcaggagg 26940 actaaggatt gattctcaaa acagacgcct tatacttgat gttagttatc cgtttgatgc 27000 tcaaaaccaa ctaaatctaa gactaggaca gggccctctt tttataaact cagcccacaa 27060 cttggatatt aactacaaca aaggccttta cttgtttaca gcttcaaaca attccaaaaa 27120 gcttgaggtt aacctaagca ctgccaaggg gttgatgttt gacgctacag ccatagccat 27180 taatgcagga gatgggcttg aatttggttc acctaatgca ccaaacacaa atcccctcaa 27240 aacaaaaatt ggccatggcc tagaatttga ttcaaacaag gctatggttc ctaaactagg 27300 aactggcctt agttttgaca gcacaggtgc cattacagta ggaaacaaaa ataatgataa 27360 gctaactttg tggaccggaa taaaccctcc acctaactgt caaattgtgg aaaacactaa 27420 tacaaatgat ggcaaactta ctttagtatt agtaaaaaat ggagggcttg ttaatggcta 27480 cgtgtctcta gttggtgtat cagacactgt gaaccaaatg ttcacacaaa agacagcaaa 27540 catccaatta agattatatt ttgactcttc tggaaatcta ttaactgagg aatcagactt 27600 aaaaattcca cttaaaaata aatcttctac agcgaccagt gaaactgtag ccagcagcaa 27660 agcctttatg ccaagtacta cagcttatcc cttcaacacc actactaggg atagtgaaaa 27720 ctacattcat ggaatatgtt actacatgac tagttatgat agaagtctat ttcccttgaa 27780 catttctata atgctaaaca gccgtatgat ttcttccaat gttgcctatg ccatacaatt 27840 tgaatggaat ctaaatgcaa gtgaatctcc agaaagcaac atagctacgc tgaccacatc 27900 cccctttttc ttttcttaca ttacagaaga cgacaactaa agaatcgttt gtgttatgtt 27960 tcaacgtgtt tatttttcaa ttgcagaaaa tttcaagtca tttttcattc agtagtatag 28020 ccccaccacc acatagctta tacagatcac cgtaccttaa tcaaactcac agaaccctag 28080 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 28140 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 28200 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 28260 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 28320 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 28380 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 28440 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 28500 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 28560 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 28620 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 28680 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 28740 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 28800 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 28860 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 28920 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 28980 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 29040 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 29100 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 29160 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 29220 acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 29280 cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 29340 tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 29400 acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 29460 cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 29520 atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 29580 aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 29640 aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 29700 ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 29760 caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 29820 agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 29880 cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 29940 cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 30000 ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 30060 ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 30120 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 30180 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 30240 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 30300 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 30360 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 30420 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 30480 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 30540 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 30600 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 30660 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 30720 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 30780 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 30840 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 30900 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 30960 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 31020 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 31080 tattgatgat g 31091 <210> 22 <211> 37678 <212> DNA <213> Artificial Sequence <220> <223> plasmid #1 vector <400> 22 ttaattaaca tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 60 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 120 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 180 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 240 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 300 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 360 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 420 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 480 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 540 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 600 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 660 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 720 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 780 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 840 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 900 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 960 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1020 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1080 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1140 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1200 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1260 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1320 ttgttgccat tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca 1380 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 1440 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 1500 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 1560 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 1620 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 1680 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1740 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1800 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1860 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1920 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1980 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2040 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2100 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2160 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 2220 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2280 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2340 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2400 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2460 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 2520 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2580 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 2640 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 2700 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 2760 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 2820 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2880 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 2940 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 3000 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 3060 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 3120 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 3180 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 3240 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta 3300 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 3360 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 3420 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 3480 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 3540 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 3600 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 3660 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 3720 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3780 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3840 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 3900 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 3960 gacaccggga ccgatccagc ctccgatttg ccaccatgtt tgtgttcctg gtgctgctgc 4020 cactggtgtc cagccagtgt gtgaacctga ccaccaggac ccaacttcct cctgcctaca 4080 ccaactcctt caccagggga gtctactacc ctgacaaggt gttcaggtcc tctgtgctgc 4140 acagcaccca ggacctgttc ctgccattct tcagcaatgt gacctggttc catgccatcc 4200 atgtgtctgg caccaatggc accaagaggt ttgacaaccc tgtgctgcca ttcaatgatg 4260 gagtctactt tgccagcaca gagaagagca acatcatcag gggctggatt tttggcacca 4320 ccctggacag caagacccag tccctgctga ttgtgaacaa tgccaccaat gtggtgatta 4380 aggtgtgtga gttccagttc tgtaatgacc cattcctggg agtctactac cacaagaaca 4440 acaagtcctg gatggagtct gagttcaggg tctactcctc tgccaacaac tgtacctttg 4500 aatatgtgag ccaaccattc ctgatggact tggagggcaa gcagggcaac ttcaagaacc 4560 tgagggagtt tgtgttcaag aacattgatg gctacttcaa gatttacagc aaacacacac 4620 caatcaacct ggtgagggac ctgccacagg gcttctctgc cttggaacca ctggtggacc 4680 tgccaattgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcctacc 4740 tgacacctgg agactcctcc tctggctgga cagcaggagc agcagcctac tatgtgggct 4800 acctccaacc aaggaccttc ctgctgaaat acaatgagaa tggcaccatc acagatgctg 4860 tggactgtgc cctggaccca ctgtctgaga ccaagtgtac cctgaaatcc ttcacagtgg 4920 agaagggcat ctaccagacc agcaacttca gggtccaacc aacagagagc attgtgaggt 4980 ttccaaacat caccaacctg tgtccatttg gagaggtgtt caatgccacc aggtttgcct 5040 ctgtctatgc ctggaacagg aagaggatta gcaactgtgt ggctgactac tctgtgctct 5100 acaactctgc ctccttcagc accttcaagt gttatggagt gagcccaacc aaactgaatg 5160 acctgtgttt caccaatgtc tatgctgact cctttgtgat taggggagat gaggtgagac 5220 agattgcccc tggacaaaca ggcaagattg ctgactacaa ctacaaactg cctgatgact 5280 tcacaggctg tgtgattgcc tggaacagca acaacctgga cagcaaggtg ggaggcaact 5340 acaactacct ctacagactg ttcaggaaga gcaacctgaa accatttgag agggacatca 5400 gcacagagat ttaccaggct ggcagcacac catgtaatgg agtggagggc ttcaactgtt 5460 actttccact ccaatcctat ggcttccaac caaccaatgg agtgggctac caaccataca 5520 gggtggtggt gctgtccttt gaactgctcc atgcccctgc cacagtgtgt ggaccaaaga 5580 agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa cttcaatgga ctgacaggca 5640 caggagtgct gacagagagc aacaagaagt tcctgccatt ccaacagttt ggcagggaca 5700 ttgctgacac cacagatgct gtgagggacc cacagacctt ggagattctg gacatcacac 5760 catgttcctt tggaggagtg tctgtgatta cacctggcac caacaccagc aaccaggtgg 5820 ctgtgctcta ccaggatgtg aactgtactg aggtgcctgt ggctatccat gctgaccaac 5880 ttacaccaac ctggagggtc tacagcacag gcagcaatgt gttccagacc agggctggct 5940 gtctgattgg agcagagcat gtgaacaact cctatgagtg tgacatccca attggagcag 6000 gcatctgtgc ctcctaccag acccagacca acagcccaag gagggcaagg tctgtggcaa 6060 gccagagcat cattgcctac acaatgagtc tgggagcaga gaactctgtg gcttacagca 6120 acaacagcat tgccatccca accaacttca ccatctctgt gaccacagag attctgcctg 6180 tgagtatgac caagacctct gtggactgta caatgtatat ctgtggagac agcacagagt 6240 gtagcaacct gctgctccaa tatggctcct tctgtaccca acttaacagg gctctgacag 6300 gcattgctgt ggaacaggac aagaacaccc aggaggtgtt tgcccaggtg aagcagattt 6360 acaagacacc tccaatcaag gactttggag gcttcaactt cagccagatt ctgcctgacc 6420 caagcaagcc aagcaagagg tccttcattg aggacctgct gttcaacaag gtgaccctgg 6480 ctgatgctgg cttcatcaag caatatggag actgtctggg agacattgct gccagggacc 6540 tgatttgtgc ccagaagttc aatggactga cagtgctgcc tccactgctg acagatgaga 6600 tgattgccca atacacctct gccctgctgg ctggcaccat cacctctggc tggacctttg 6660 gagcaggagc agccctccaa atcccatttg ctatgcagat ggcttacagg ttcaatggca 6720 ttggagtgac ccagaatgtg ctctatgaga accagaaact gattgccaac cagttcaact 6780 ctgccattgg caagattcag gactccctgt ccagcacagc ctctgccctg ggcaaactcc 6840 aagatgtggt gaaccagaat gcccaggctc tgaacaccct ggtgaagcaa ctttccagca 6900 actttggagc catctcctct gtgctgaatg acatcctgag cagactggac aaggtggagg 6960 ctgaggtcca gattgacaga ctgattacag gcagactcca atccctccaa acctatgtga 7020 cccaacaact tatcagggct gctgagatta gggcatctgc caacctggct gccaccaaga 7080 tgagtgagtg tgtgctggga caaagcaaga gggtggactt ctgtggcaag ggctaccacc 7140 tgatgagttt tccacagtct gcccctcatg gagtggtgtt cctgcatgtg acctatgtgc 7200 ctgcccagga gaagaacttc accacagccc ctgccatctg ccatgatggc aaggctcact 7260 ttccaaggga gggagtgttt gtgagcaatg gcacccactg gtttgtgacc cagaggaact 7320 tctatgaacc acagattatc accacagaca acacctttgt gtctggcaac tgtgatgtgg 7380 tgattggcat tgtgaacaac acagtctatg acccactcca acctgaactg gactccttca 7440 aggaggaact ggacaaatac ttcaagaacc acaccagccc tgatgtggac ctgggagaca 7500 tctctggcat caatgcctct gtggtgaaca tccagaagga gattgacaga ctgaatgagg 7560 tggctaagaa cctgaatgag tccctgattg acctccaaga actgggcaaa tatgaacaat 7620 acatcaagtg gccatgaaaa ttgatcataa tcagccatac cacatttgta gaggttttac 7680 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 7740 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7800 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7860 atgtatctta acgcggatct gggcgtggtt aagggtggga aagaatatat aaggtggggg 7920 tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca ccaactcgtt 7980 tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg ccggggtgcg 8040 tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa actctactac 8100 cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg ccgccgcttc 8160 agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga gcccgcttgc 8220 aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc ttttggcaca 8280 attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg atctgcgcca 8340 gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca taaataaaaa 8400 accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt taggggtttt 8460 gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc 8520 caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg 8580 gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc 8640 gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag 8700 gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg 8760 ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct 8820 ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt 8880 gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag 8940 attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa 9000 gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat 9060 ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg 9120 ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat 9180 gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga 9240 aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat 9300 taccggctgc aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc 9360 cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg 9420 ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc 9480 gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc 9540 ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg 9600 ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac 9660 gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc 9720 gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg 9780 ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg 9840 tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt 9900 ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg 9960 caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca 10020 aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg 10080 tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg 10140 tcctcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag 10200 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact 10260 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag 10320 gtgattggtt tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag 10380 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt 10440 tggggtgagt actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc 10500 aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca 10560 tccatctggt cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag 10620 agggcgttgg acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg 10680 cgctccttgg ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga 10740 aagacggtgg tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg 10800 acaaggtcaa cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg 10860 ccgcccttgc gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct 10920 gcgtccacgg taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct 10980 tgcaagtcta gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt 11040 gggggacccc atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa 11100 acgtagaggg gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg 11160 ctggcgcgca cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg 11220 ctacgggcgg gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat 11280 gatatggttg gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc 11340 acgaaggagg cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct 11400 agggcgcagt agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc 11460 cacagctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac 11520 ccgtcggcct ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg 11580 cagcatccct tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg 11640 gtgagcgcaa aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg 11700 tcgcatccgc cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc 11760 agggcgaagg tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg 11820 atgcggaagg gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc 11880 tcgtcaaagc cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc 11940 ttgatggaag gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg 12000 tgctctgaaa gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg 12060 tcacgggcca ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc 12120 attttttctg gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca 12180 aggttcgcgg ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg 12240 accagcatga agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca 12300 tcgtaggtga caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc 12360 tcccgccacc aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg 12420 gccgaacact cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc 12480 tgtacatcct gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg 12540 agcccctcgc ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg 12600 tctggctgct cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc 12660 cagatgtccg cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc 12720 atggtctgga gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat 12780 agacgggtca gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg 12840 gcggcgtcga tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc 12900 gggcggtggg ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag 12960 cccccggagg tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc 13020 cgcgcgcggg caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc 13080 ggttgatctc ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgaacc 13140 tgaaagagag ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct 13200 cctgcacgtc tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt 13260 cctcctggag atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc 13320 gggccatgag ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca 13380 cgcccccttc ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc 13440 gggcgaagac ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt 13500 gttctgccac gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca 13560 aggcctcaag gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt 13620 tgcgcgccga cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc 13680 gcacctcgcg ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa 13740 gggcctcccc ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac 13800 ggcgcaccgg gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg 13860 tctcggtgac ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt 13920 cccggttatg ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc 13980 tcaacaattg ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg 14040 gatcggaaaa cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca 14100 ccgtggcggg cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga 14160 tgtaattaaa gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg 14220 gtccggcctg ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc 14280 gcaggtcttt gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct 14340 cttgtcctgc atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc 14400 gccctcttcc tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt 14460 cggcgacaac gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt 14520 catccatgtc cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca 14580 taacggacca gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg 14640 agtaagccct cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca 14700 ccaaaaagtg cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg 14760 gggcgagatc ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga 14820 tgccggcggc ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca 14880 gcggcaaaaa gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga 14940 cgctctagcg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc tggtggataa 15000 attcgcaagg gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg ccgtccgccg 15060 tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag acaacggggg 15120 agtgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt tttggccact 15180 ggccgcgcgc agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg ctcgctccct 15240 gtagccggag ggttattttc caagggttga gtcgcgggac ccccggttcg agtctcggac 15300 cggccggact gcggcgaacg ggggtttgcc tccccgtcat gcaagacccc gcttgcaaat 15360 tcctccggaa acagggacga gccccttttt tgcttttccc agatgcatcc ggtgctgcgg 15420 cagatgcgcc cccctcctca gcagcggcaa gagcaagagc agcggcagac atgcagggca 15480 ccctcccctc ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc ggcagcagat 15540 ggtgattacg aacccccgcg gcgccgggcc cggcactacc tggacttgga ggagggcgag 15600 ggcctggcgc ggctaggagc gccctctcct gagcggcacc caagggtgca gctgaagcgt 15660 gatacgcgtg aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga gggagaggag 15720 cccgaggaga tgcgggatcg aaagttccac gcagggcgcg agctgcggca tggcctgaat 15780 cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg gattagtccc 15840 gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcat acgagcagac ggtgaaccag 15900 gagattaact ttcaaaaaag ctttaacaac cacgtgcgta cgcttgtggc gcgcgaggag 15960 gtggctatag gactgatgca tctgtgggac tttgtaagcg cgctggagca aaacccaaat 16020 agcaagccgc tcatggcgca gctgttcctt atagtgcagc acagcaggga caacgaggca 16080 ttcagggatg cgctgctaaa catagtagag cccgagggcc gctggctgct cgatttgata 16140 aacatcctgc agagcatagt ggtgcaggag cgcagcttga gcctggctga caaggtggcc 16200 gccatcaact attccatgct tagcctgggc aagttttacg cccgcaagat ataccatacc 16260 ccttacgttc ccatagacaa ggaggtaaag atcgaggggt tctacatgcg catggcgctg 16320 aaggtgctta ccttgagcga cgacctgggc gtttatcgca acgagcgcat ccacaaggcc 16380 gtgagcgtga gccggcggcg cgagctcagc gaccgcgagc tgatgcacag cctgcaaagg 16440 gccctggctg gcacgggcag cggcgataga gaggccgagt cctactttga cgcgggcgct 16500 gacctgcgct gggccccaag ccgacgcgcc ctggaggcag ctggggccgg acctgggctg 16560 gcggtggcac ccgcgcgcgc tggcaacgtc ggcggcgtgg aggaatatga cgaggacgat 16620 gagtacgagc cagaggacgg cgagtactaa gcggtgatgt ttctgatcag atgatgcaag 16680 acgcaacgga cccggcggtg cgggcggcgc tgcagagcca gccgtccggc cttaactcca 16740 cggacgactg gcgccaggtc atggaccgca tcatgtcgct gactgcgcgc aatcctgacg 16800 cgttccggca gcagccgcag gccaaccggc tctccgcaat tctggaagcg gtggtcccgg 16860 cgcgcgcaaa ccccacgcac gagaaggtgc tggcgatcgt aaacgcgctg gccgaaaaca 16920 gggccatccg gcccgacgag gccggcctgg tctacgacgc gctgcttcag cgcgtggctc 16980 gttacaacag cggcaacgtg cagaccaacc tggaccggct ggtgggggat gtgcgcgagg 17040 ccgtggcgca gcgtgagcgc gcgcagcagc agggcaacct gggctccatg gttgcactaa 17100 acgccttcct gagtacacag cccgccaacg tgccgcgggg acaggaggac tacaccaact 17160 ttgtgagcgc actgcggcta atggtgactg agacaccgca aagtgaggtg taccagtctg 17220 ggccagacta ttttttccag accagtagac aaggcctgca gaccgtaaac ctgagccagg 17280 ctttcaaaaa cttgcagggg ctgtgggggg tgcgggctcc cacaggcgac cgcgcgaccg 17340 tgtctagctt gctgacgccc aactcgcgcc tgttgctgct gctaatagcg cccttcacgg 17400 acagtggcag cgtgtcccgg gacacatacc taggtcactt gctgacactg taccgcgagg 17460 ccataggtca ggcgcatgtg gacgagcata ctttccagga gattacaagt gtcagccgcg 17520 cgctggggca ggaggacacg ggcagcctgg aggcaaccct aaactacctg ctgaccaacc 17580 ggcggcagaa gatcccctcg ttgcacagtt taaacagcga ggaggagcgc attttgcgct 17640 acgtgcagca gagcgtgagc cttaacctga tgcgcgacgg ggtaacgccc agcgtggcgc 17700 tggacatgac cgcgcgcaac atggaaccgg gcatgtatgc ctcaaaccgg ccgtttatca 17760 accgcctaat ggactacttg catcgcgcgg ccgccgtgaa ccccgagtat ttcaccaatg 17820 ccatcttgaa cccgcactgg ctaccgcccc ctggtttcta caccggggga ttcgaggtgc 17880 ccgagggtaa cgatggattc ctctgggacg acatagacga cagcgtgttt tccccgcaac 17940 cgcagaccct gctagagttg caacagcgcg agcaggcaga ggcggcgctg cgaaaggaaa 18000 gcttccgcag gccaagcagc ttgtccgatc taggcgctgc ggccccgcgg tcagatgcta 18060 gtagcccatt tccaagcttg atagggtctc ttaccagcac tcgcaccacc cgcccgcgcc 18120 tgctgggcga ggaggagtac ctaaacaact cgctgctgca gccgcagcgc gaaaaaaacc 18180 tgcctccggc atttcccaac aacgggatag agagcctagt ggacaagatg agtagatgga 18240 agacgtacgc gcaggagcac agggacgtgc caggcccgcg cccgcccacc cgtcgtcaaa 18300 ggcacgaccg tcagcggggt ctggtgtggg aggacgatga ctcggcagac gacagcagcg 18360 tcctggattt gggagggagt ggcaacccgt ttgcgcacct tcgccccagg ctggggagaa 18420 tgttttaaaa aaaaaaaagc atgatgcaaa ataaaaaact caccaaggcc atggcaccga 18480 gcgttggttt tcttgtattc cccttagtat gcggcgcgcg gcgatgtatg aggaaggtcc 18540 tcctccctcc tacgagagtg tggtgagcgc ggcgccagtg gcggcggcgc tgggttctcc 18600 cttcgatgct cccctggacc cgccgtttgt gcctccgcgg tacctgcggc ctaccggggg 18660 gagaaacagc atccgttact ctgagttggc acccctattc gacaccaccc gtgtgtacct 18720 ggtggacaac aagtcaacgg atgtggcatc cctgaactac cagaacgacc acagcaactt 18780 tctgaccacg gtcattcaaa acaatgacta cagcccgggg gaggcaagca cacagaccat 18840 caatcttgac gaccggtcgc actggggcgg cgacctgaaa accatcctgc ataccaacat 18900 gccaaatgtg aacgagttca tgtttaccaa taagtttaag gcgcgggtga tggtgtcgcg 18960 cttgcctact aaggacaatc aggtggagct gaaatacgag tgggtggagt tcacgctgcc 19020 cgagggcaac tactccgaga ccatgaccat agaccttatg aacaacgcga tcgtggagca 19080 ctacttgaaa gtgggcagac agaacggggt tctggaaagc gacatcgggg taaagtttga 19140 cacccgcaac ttcagactgg ggtttgaccc cgtcactggt cttgtcatgc ctggggtata 19200 tacaaacgaa gccttccatc cagacatcat tttgctgcca ggatgcgggg tggacttcac 19260 ccacagccgc ctgagcaact tgttgggcat ccgcaagcgg caacccttcc aggagggctt 19320 taggatcacc tacgatgatc tggagggtgg taacattccc gcactgttgg atgtggacgc 19380 ctaccaggcg agcttgaaag atgacaccga acagggcggg ggtggcgcag gcggcagcaa 19440 cagcagtggc agcggcgcgg aagagaactc caacgcggca gccgcggcaa tgcagccggt 19500 ggaggacatg aacgatcatg ccattcgcgg cgacaccttt gccacacggg ctgaggagaa 19560 gcgcgctgag gccgaagcag cggccgaagc tgccgccccc gctgcgcaac ccgaggtcga 19620 gaagcctcag aagaaaccgg tgatcaaacc cctgacagag gacagcaaga aacgcagtta 19680 caacctaata agcaatgaca gcaccttcac ccagtaccgc agctggtacc ttgcatacaa 19740 ctacggcgac cctcagaccg gaatccgctc atggaccctg ctttgcactc ctgacgtaac 19800 ctgcggctcg gagcaggtct actggtcgtt gccagacatg atgcaagacc ccgtgacctt 19860 ccgctccacg cgccagatca gcaactttcc ggtggtgggc gccgagctgt tgcccgtgca 19920 ctccaagagc ttctacaacg accaggccgt ctactcccaa ctcatccgcc agtttacctc 19980 tctgacccac gtgttcaatc gctttcccga gaaccagatt ttggcgcgcc cgccagcccc 20040 caccatcacc accgtcagtg aaaacgttcc tgctctcaca gatcacggga cgctaccgct 20100 gcgcaacagc atcggaggag tccagcgagt gaccattact gacgccagac gccgcacctg 20160 cccctacgtt tacaaggccc tgggcatagt ctcgccgcgc gtcctatcga gccgcacttt 20220 ttgagcaagc atgtccatcc ttatatcgcc cagcaataac acaggctggg gcctgcgctt 20280 cccaagcaag atgtttggcg gggccaagaa gcgctccgac caacacccag tgcgcgtgcg 20340 cgggcactac cgcgcgccct ggggcgcgca caaacgcggc cgcactgggc gcaccaccgt 20400 cgatgacgcc atcgacgcgg tggtggagga ggcgcgcaac tacacgccca cgccgccacc 20460 agtgtccaca gtggacgcgg ccattcagac cgtggtgcgc ggagcccggc gctatgctaa 20520 aatgaagaga cggcggaggc gcgtagcacg tcgccaccgc cgccgacccg gcactgccgc 20580 ccaacgcgcg gcggcggccc tgcttaaccg cgcacgtcgc accggccgac gggcggccat 20640 gcgggccgct cgaaggctgg ccgcgggtat tgtcactgtg ccccccaggt ccaggcgacg 20700 agcggccgcc gcagcagccg cggccattag tgctatgact cagggtcgca ggggcaacgt 20760 gtattgggtg cgcgactcgg ttagcggcct gcgcgtgccc gtgcgcaccc gccccccgcg 20820 caactagatt gcaagaaaaa actacttaga ctcgtactgt tgtatgtatc cagcggcggc 20880 ggcgcgcaac gaagctatgt ccaagcgcaa aatcaaagaa gagatgctcc aggtcatcgc 20940 gccggagatc tatggccccc cgaagaagga agagcaggat tacaagcccc gaaagctaaa 21000 gcgggtcaaa aagaaaaaga aagatgatga tgatgaactt gacgacgagg tggaactgct 21060 gcacgctacc gcgcccaggc gacgggtaca gtggaaaggt cgacgcgtaa aacgtgtttt 21120 gcgacccggc accaccgtag tctttacgcc cggtgagcgc tccacccgca cctacaagcg 21180 cgtgtatgat gaggtgtacg gcgacgagga cctgcttgag caggccaacg agcgcctcgg 21240 ggagtttgcc tacggaaagc ggcataagga catgctggcg ttgccgctgg acgagggcaa 21300 cccaacacct agcctaaagc ccgtaacact gcagcaggtg ctgcccgcgc ttgcaccgtc 21360 cgaagaaaag cgcggcctaa agcgcgagtc tggtgacttg gcacccaccg tgcagctgat 21420 ggtacccaag cgccagcgac tggaagatgt cttggaaaaa atgaccgtgg aacctgggct 21480 ggagcccgag gtccgcgtgc ggccaatcaa gcaggtggcg ccgggactgg gcgtgcagac 21540 cgtggacgtt cagataccca ctaccagtag caccagtatt gccaccgcca cagagggcat 21600 ggagacacaa acgtccccgg ttgcctcagc ggtggcggat gccgcggtgc aggcggtcgc 21660 tgcggccgcg tccaagacct ctacggaggt gcaaacggac ccgtggatgt ttcgcgtttc 21720 agccccccgg cgcccgcgcc gttcgaggaa gtacggcgcc gccagcgcgc tactgcccga 21780 atatgcccta catccttcca ttgcgcctac ccccggctat cgtggctaca cctaccgccc 21840 cagaagacga gcaactaccc gacgccgaac caccactgga acccgccgcc gccgtcgccg 21900 tcgccagccc gtgctggccc cgatttccgt gcgcagggtg gctcgcgaag gaggcaggac 21960 cctggtgctg ccaacagcgc gctaccaccc cagcatcgtt taaaagccgg tctttgtggt 22020 tcttgcagat atggccctca cctgccgcct ccgtttcccg gtgccgggat tccgaggaag 22080 aatgcaccgt aggaggggca tggccggcca cggcctgacg ggcggcatgc gtcgtgcgca 22140 ccaccggcgg cggcgcgcgt cgcaccgtcg catgcgcggc ggtatcctgc ccctccttat 22200 tccactgatc gccgcggcga ttggcgccgt gcccggaatt gcatccgtgg ccttgcaggc 22260 gcagagacac tgattaaaaa caagttgcat gtggaaaaat caaaataaaa agtctggact 22320 ctcacgctcg cttggtcctg taactatttt gtagaatgga agacatcaac tttgcgtctc 22380 tggccccgcg acacggctcg cgcccgttca tgggaaactg gcaagatatc ggcaccagca 22440 atatgagcgg tggcgccttc agctggggct cgctgtggag cggcattaaa aatttcggtt 22500 ccaccgttaa gaactatggc agcaaggcct ggaacagcag cacaggccag atgctgaggg 22560 ataagttgaa agagcaaaat ttccaacaaa aggtggtaga tggcctggcc tctggcatta 22620 gcggggtggt ggacctggcc aaccaggcag tgcaaaataa gattaacagt aagcttgatc 22680 cccgccctcc cgtagaggag cctccaccgg ccgtggagac agtgtctcca gaggggcgtg 22740 gcgaaaagcg tccgcgcccc gacagggaag aaactctggt gacgcaaata gacgagcctc 22800 cctcgtacga ggaggcacta aagcaaggcc tgcccaccac ccgtcccatc gcgcccatgg 22860 ctaccggagt gctgggccag cacacacccg taacgctgga cctgcctccc cccgccgaca 22920 cccagcagaa acctgtgctg ccaggcccga ccgccgttgt tgtaacccgt cctagccgcg 22980 cgtccctgcg ccgcgccgcc agcggtccgc gatcgttgcg gcccgtagcc agtggcaact 23040 ggcaaagcac actgaacagc atcgtgggtc tgggggtgca atccctgaag cgccgacgat 23100 gcttctgata gctaacgtgt cgtatgtgtg tcatgtatgc gtccatgtcg ccgccagagg 23160 agctgctgag ccgccgcgcg cccgctttcc aagatggcta ccccttcgat gatgccgcag 23220 tggtcttaca tgcacatctc gggccaggac gcctcggagt acctgagccc cgggctggtg 23280 cagtttgccc gcgccaccga gacgtacttc agcctgaata acaagtttag aaaccccacg 23340 gtggcgccta cgcacgacgt gaccacagac cggtcccagc gtttgacgct gcggttcatc 23400 cctgtggacc gtgaggatac tgcgtactcg tacaaggcgc ggttcaccct agctgtgggt 23460 gataaccgtg tgctggacat ggcttccacg tactttgaca tccgcggcgt gctggacagg 23520 ggccctactt ttaagcccta ctctggcact gcctacaacg ccctggctcc caagggtgcc 23580 ccaaatcctt gcgaatggga tgaagctgct actgctcttg aaataaacct agaagaagag 23640 gacgatgaca acgaagacga agtagacgag caagctgagc agcaaaaaac tcacgtattt 23700 gggcaggcgc cttattctgg tataaatatt acaaaggagg gtattcaaat aggtgtcgaa 23760 ggtcaaacac ctaaatatgc cgataaaaca tttcaacctg aacctcaaat aggagaatct 23820 cagtggtacg aaacagaaat taatcatgca gctgggagag tcctaaaaaa gactacccca 23880 atgaaaccat gttacggttc atatgcaaaa cccacaaatg aaaatggagg gcaaggcatt 23940 cttgtaaagc aacaaaatgg aaagctagaa agtcaagtgg aaatgcaatt tttctcaact 24000 actgaggcag ccgcaggcaa tggtgataac ttgactccta aagtggtatt gtacagtgaa 24060 gatgtagata tagaaacccc agacactcat atttcttaca tgcccactat taaggaaggt 24120 aactcacgag aactaatggg ccaacaatct atgcccaaca ggcctaatta cattgctttt 24180 agggacaatt ttattggtct aatgtattac aacagcacgg gtaatatggg tgttctggcg 24240 ggccaagcat cgcagttgaa tgctgttgta gatttgcaag acagaaacac agagctttca 24300 taccagcttt tgcttgattc cattggtgat agaaccaggt acttttctat gtggaatcag 24360 gctgttgaca gctatgatcc agatgttaga attattgaaa atcatggaac tgaagatgaa 24420 cttccaaatt actgctttcc actgggaggt gtgattaata cagagactct taccaaggta 24480 aaacctaaaa caggtcagga aaatggatgg gaaaaagatg ctacagaatt ttcagataaa 24540 aatgaaataa gagttggaaa taattttgcc atggaaatca atctaaatgc caacctgtgg 24600 agaaatttcc tgtactccaa catagcgctg tatttgcccg acaagctaaa gtacagtcct 24660 tccaacgtaa aaatttctga taacccaaac acctacgact acatgaacaa gcgagtggtg 24720 gctcccgggc tagtggactg ctacattaac cttggagcac gctggtccct tgactatatg 24780 gacaacgtca acccatttaa ccaccaccgc aatgctggcc tgcgctaccg ctcaatgttg 24840 ctgggcaatg gtcgctatgt gcccttccac atccaggtgc ctcagaagtt ctttgccatt 24900 aaaaacctcc ttctcctgcc gggctcatac acctacgagt ggaacttcag gaaggatgtt 24960 aacatggttc tgcagagctc cctaggaaat gacctaaggg ttgacggagc cagcattaag 25020 tttgatagca tttgccttta cgccaccttc ttccccatgg cccacaacac cgcctccacg 25080 cttgaggcca tgcttagaaa cgacaccaac gaccagtcct ttaacgacta tctctccgcc 25140 gccaacatgc tctaccctat acccgccaac gctaccaacg tgcccatatc catcccctcc 25200 cgcaactggg cggctttccg cggctgggcc ttcacgcgcc ttaagactaa ggaaacccca 25260 tcactgggct cgggctacga cccttattac acctactctg gctctatacc ctacctagat 25320 ggaacctttt acctcaacca cacctttaag aaggtggcca ttacctttga ctcttctgtc 25380 agctggcctg gcaatgaccg cctgcttacc cccaacgagt ttgaaattaa gcgctcagtt 25440 gacggggagg gttacaacgt tgcccagtgt aacatgacca aagactggtt cctggtacaa 25500 atgctagcta actataacat tggctaccag ggcttctata tcccagagag ctacaaggac 25560 cgcatgtact ccttctttag aaacttccag cccatgagcc gtcaggtggt ggatgatact 25620 aaatacaagg actaccaaca ggtgggcatc ctacaccaac acaacaactc tggatttgtt 25680 ggctaccttg cccccaccat gcgcgaagga caggcctacc ctgctaactt cccctatccg 25740 cttataggca agaccgcagt tgacagcatt acccagaaaa agtttctttg cgatcgcacc 25800 ctttggcgca tcccattctc cagtaacttt atgtccatgg gcgcactcac agacctgggc 25860 caaaaccttc tctacgccaa ctccgcccac gcgctagaca tgacttttga ggtggatccc 25920 atggacgagc ccacccttct ttatgttttg tttgaagtct ttgacgtggt ccgtgtgcac 25980 cagccgcacc gcggcgtcat cgaaaccgtg tacctgcgca cgcccttctc ggccggcaac 26040 gccacaacat aaagaagcaa gcaacatcaa caacagctgc cgccatgggc tccagtgagc 26100 aggaactgaa agccattgtc aaagatcttg gttgtgggcc atattttttg ggcacctatg 26160 acaagcgctt tccaggcttt gtttctccac acaagctcgc ctgcgccata gtcaatacgg 26220 ccggtcgcga gactgggggc gtacactgga tggcctttgc ctggaacccg cactcaaaaa 26280 catgctacct ctttgagccc tttggctttt ctgaccagcg actcaagcag gtttaccagt 26340 ttgagtacga gtcactcctg cgccgtagcg ccattgcttc ttcccccgac cgctgtataa 26400 cgctggaaaa gtccacccaa agcgtacagg ggcccaactc ggccgcctgt ggactattct 26460 gctgcatgtt tctccacgcc tttgccaact ggccccaaac tcccatggat cacaacccca 26520 ccatgaacct tattaccggg gtacccaact ccatgctcaa cagtccccag gtacagccca 26580 ccctgcgtcg caaccaggaa cagctctaca gcttcctgga gcgccactcg ccctacttcc 26640 gcagccacag tgcgcagatt aggagcgcca cttctttttg tcacttgaaa aacatgtaaa 26700 aataatgtac tagagacact ttcaataaag gcaaatgctt ttatttgtac actctcgggt 26760 gattatttac ccccaccctt gccgtctgcg ccgtttaaaa atcaaagggg ttctgccgcg 26820 catcgctatg cgccactggc agggacacgt tgcgatactg gtgtttagtg ctccacttaa 26880 actcaggcac aaccatccgc ggcagctcgg tgaagttttc actccacagg ctgcgcacca 26940 tcaccaacgc gtttagcagg tcgggcgccg atatcttgaa gtcgcagttg gggcctccgc 27000 cctgcgcgcg cgagttgcga tacacagggt tgcagcactg gaacactatc agcgccgggt 27060 ggtgcacgct ggccagcacg ctcttgtcgg agatcagatc cgcgtccagg tcctccgcgt 27120 tgctcagggc gaacggagtc aactttggta gctgccttcc caaaaagggc gcgtgcccag 27180 gctttgagtt gcactcgcac cgtagtggca tcaaaaggtg accgtgcccg gtctgggcgt 27240 taggatacag cgcctgcata aaagccttga tctgcttaaa agccacctga gcctttgcgc 27300 cttcagagaa gaacatgccg caagacttgc cggaaaactg attggccgga caggccgcgt 27360 cgtgcacgca gcaccttgcg tcggtgttgg agatctgcac cacatttcgg ccccaccggt 27420 tcttcacgat cttggccttg ctagactgct ccttcagcgc gcgctgcccg ttttcgctcg 27480 tcacatccat ttcaatcacg tgctccttat ttatcataat gcttccgtgt agacacttaa 27540 gctcgccttc gatctcagcg cagcggtgca gccacaacgc gcagcccgtg ggctcgtgat 27600 gcttgtaggt cacctctgca aacgactgca ggtacgcctg caggaatcgc cccatcatcg 27660 tcacaaaggt cttgttgctg gtgaaggtca gctgcaaccc gcggtgctcc tcgttcagcc 27720 aggtcttgca tacggccgcc agagcttcca cttggtcagg cagtagtttg aagttcgcct 27780 ttagatcgtt atccacgtgg tacttgtcca tcagcgcgcg cgcagcctcc atgcccttct 27840 cccacgcaga cacgatcggc acactcagcg ggttcatcac cgtaatttca ctttccgctt 27900 cgctgggctc ttcctcttcc tcttgcgtcc gcataccacg cgccactggg tcgtcttcat 27960 tcagccgccg cactgtgcgc ttacctcctt tgccatgctt gattagcacc ggtgggttgc 28020 tgaaacccac catttgtagc gccacatctt ctctttcttc ctcgctgtcc acgattacct 28080 ctggtgatgg cgggcgctcg ggcttgggag aagggcgctt ctttttcttc ttgggcgcaa 28140 tggccaaatc cgccgccgag gtcgatggcc gcgggctggg tgtgcgcggc accagcgcgt 28200 cttgtgatga gtcttcctcg tcctcggact cgatacgccg cctcatccgc ttttttgggg 28260 gcgcccgggg aggcggcggc gacggggacg gggacgacac gtcctccatg gttgggggac 28320 gtcgcgccgc accgcgtccg cgctcggggg tggtttcgcg ctgctcctct tcccgactgg 28380 ccatttcctt ctcctatagg cagaaaaaga tcatggagtc agtcgagaag aaggacagcc 28440 taaccgcccc ctctgagttc gccaccaccg cctccaccga tgccgccaac gcgcctacca 28500 ccttccccgt cgaggcaccc ccgcttgagg aggaggaagt gattatcgag caggacccag 28560 gttttgtaag cgaagacgac gaggaccgct cagtaccaac agaggataaa aagcaagacc 28620 aggacaacgc agaggcaaac gaggaacaag tcgggcgggg ggacgaaagg catggcgact 28680 acctagatgt gggagacgac gtgctgttga agcatctgca gcgccagtgc gccattatct 28740 gcgacgcgtt gcaagagcgc agcgatgtgc ccctcgccat agcggatgtc agccttgcct 28800 acgaacgcca cctattctca ccgcgcgtac cccccaaacg ccaagaaaac ggcacatgcg 28860 agcccaaccc gcgcctcaac ttctaccccg tatttgccgt gccagaggtg cttgccacct 28920 atcacatctt tttccaaaac tgcaagatac ccctatcctg ccgtgccaac cgcagccgag 28980 cggacaagca gctggccttg cggcagggcg ctgtcatacc tgatatcgcc tcgctcaacg 29040 aagtgccaaa aatctttgag ggtcttggac gcgacgagaa gcgcgcggca aacgctctgc 29100 aacaggaaaa cagcgaaaat gaaagtcact ctggagtgtt ggtggaactc gagggtgaca 29160 acgcgcgcct agccgtacta aaacgcagca tcgaggtcac ccactttgcc tacccggcac 29220 ttaacctacc ccccaaggtc atgagcacag tcatgagtga gctgatcgtg cgccgtgcgc 29280 agcccctgga gagggatgca aatttgcaag aacaaacaga ggagggccta cccgcagttg 29340 gcgacgagca gctagcgcgc tggcttcaaa cgcgcgagcc tgccgacttg gaggagcgac 29400 gcaaactaat gatggccgca gtgctcgtta ccgtggagct tgagtgcatg cagcggttct 29460 ttgctgaccc ggagatgcag cgcaagctag aggaaacatt gcactacacc tttcgacagg 29520 gctacgtacg ccaggcctgc aagatctcca acgtggagct ctgcaacctg gtctcctacc 29580 ttggaatttt gcacgaaaac cgccttgggc aaaacgtgct tcattccacg ctcaagggcg 29640 aggcgcgccg cgactacgtc cgcgactgcg tttacttatt tctatgctac acctggcaga 29700 cggccatggg cgtttggcag cagtgcttgg aggagtgcaa cctcaaggag ctgcagaaac 29760 tgctaaagca aaacttgaag gacctatgga cggccttcaa cgagcgctcc gtggccgcgc 29820 acctggcgga catcattttc cccgaacgcc tgcttaaaac cctgcaacag ggtctgccag 29880 acttcaccag tcaaagcatg ttgcagaact ttaggaactt tatcctagag cgctcaggaa 29940 tcttgcccgc cacctgctgt gcacttccta gcgactttgt gcccattaag taccgcgaat 30000 gccctccgcc gctttggggc cactgctacc ttctgcagct agccaactac cttgcctacc 30060 actctgacat aatggaagac gtgagcggtg acggtctact ggagtgtcac tgtcgctgca 30120 acctatgcac cccgcaccgc tccctggttt gcaattcgca gctgcttaac gaaagtcaaa 30180 ttatcggtac ctttgagctg cagggtccct cgcctgacga aaagtccgcg gctccggggt 30240 tgaaactcac tccggggctg tggacgtcgg cttaccttcg caaatttgta cctgaggact 30300 accacgccca cgagattagg ttctacgaag accaatcccg cccgcctaat gcggagctta 30360 ccgcctgcgt cattacccag ggccacattc ttggccaatt gcaagccatc aacaaagccc 30420 gccaagagtt tctgctacga aagggacggg gggtttactt ggacccccag tccggcgagg 30480 agctcaaccc aatccccccg ccgccgcagc cctatcagca gcagccgcgg gcccttgctt 30540 cccaggatgg cacccaaaaa gaagctgcag ctgccgccgc cacccacgga cgaggaggaa 30600 tactgggaca gtcaggcaga ggaggttttg gacgaggagg aggaggacat gatggaagac 30660 tgggagagcc tagacgagga agcttccgag gtcgaagagg tgtcagacga aacaccgtca 30720 ccctcggtcg cattcccctc gccggcgccc cagaaatcgg caaccggttc cagcatggct 30780 acaacctccg ctcctcaggc gccgccggca ctgcccgttc gccgacccaa ccgtagatgg 30840 gacaccactg gaaccagggc cggtaagtcc aagcagccgc cgccgttagc ccaagagcaa 30900 caacagcgcc aaggctaccg ctcatggcgc gggcacaaga acgccatagt tgcttgcttg 30960 caagactgtg ggggcaacat ctccttcgcc cgccgctttc ttctctacca tcacggcgtg 31020 gccttccccc gtaacatcct gcattactac cgtcatctct acagcccata ctgcaccggc 31080 ggcagcggca gcaacagcag cggccacaca gaagcaaagg cgaccggata gcaagactct 31140 gacaaagccc aagaaatcca cagcggcggc agcagcagga ggaggagcgc tgcgtctggc 31200 gcccaacgaa cccgtatcga cccgcgagct tagaaacagg atttttccca ctctgtatgc 31260 tatatttcaa cagagcaggg gccaagaaca agagctgaaa ataaaaaaca ggtctctgcg 31320 atccctcacc cgcagctgcc tgtatcacaa aagcgaagat cagcttcggc gcacgctgga 31380 agacgcggag gctctcttca gtaaatactg cgcgctgact cttaaggact agtttcgcgc 31440 cctttctcaa atttaagcgc gaaaactacg tcatctccag cggccacacc cggcgccagc 31500 acctgttgtc agcgccatta tgagcaagga aattcccacg ccctacatgt ggagttacca 31560 gccacaaatg ggacttgcgg ctggagctgc ccaagactac tcaacccgaa taaactacat 31620 gagcgcggga ccccacatga tatcccgggt caacggaata cgcgcccacc gaaaccgaat 31680 tctcctggaa caggcggcta ttaccaccac acctcgtaat aaccttaatc cccgtagttg 31740 gcccgctgcc ctggtgtacc aggaaagtcc cgctcccacc actgtggtac ttcccagaga 31800 cgcccaggcc gaagttcaga tgactaactc aggggcgcag cttgcgggcg gctttcgtca 31860 cagggtgcgg tcgcccgggc agggtataac tcacctgaca atcagagggc gaggtattca 31920 gctcaacgac gagtcggtga gctcctcgct tggtctccgt ccggacggga catttcagat 31980 cggcggcgcc ggccgctctt cattcacgcc tcgtcaggca atcctaactc tgcagacctc 32040 gtcctctgag ccgcgctctg gaggcattgg aactctgcaa tttattgagg agtttgtgcc 32100 atcggtctac tttaacccct tctcgggacc tcccggccac tatccggatc aatttattcc 32160 taactttgac gcggtaaagg actcggcgga cggctacgac tgaatgttaa gtggagaggc 32220 agagcaactg cgcctgaaac acctggtcca ctgtcgccgc cacaagtgct ttgcccgcga 32280 ctccggtgag ttttgctact ttgaattgcc cgaggatcat atcgagggcc cggcgcacgg 32340 cgtccggctt accgcccagg gagagcttgc ccgtagcctg attcgggagt ttacccagcg 32400 ccccctgcta gttgagcggg acaggggacc ctgtgttctc actgtgattt gcaactgtcc 32460 taaccctgga ttacatcaag atcctctagt taatgtcagg tcgcctaagt cgattaacta 32520 gagtacccgg ggatcttatt ccctttaact aataaaaaaa aataataaag catcacttac 32580 ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt gccctcctcc 32640 cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct aaatggaatg 32700 tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt gcagatgaag 32760 cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac ggaaaccggt 32820 cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt tcaagagagt 32880 ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa tggcatgctt 32940 gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac ctcccaaaat 33000 gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct ggaaatatct 33060 gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc tctaatggtc 33120 gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga ctccaaactt 33180 agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct gcaaacatca 33240 ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc ccctctaact 33300 actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca aaatggaaaa 33360 ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac tttgaccgta 33420 gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt tactggagcc 33480 ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact aaggattgat 33540 tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca aaaccaacta 33600 aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt ggatattaac 33660 tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct tgaggttaac 33720 ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa tgcaggagat 33780 gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac aaaaattggc 33840 catggcctag aatttgattc aaacaaggct atggttccta aactaggaac tggccttagt 33900 tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct aactttgtgg 33960 accggaataa accctccacc taactgtcaa attgtggaaa acactaatac aaatgatggc 34020 aaacttactt tagtattagt aaaaaatgga gggcttgtta atggctacgt gtctctagtt 34080 ggtgtatcag acactgtgaa ccaaatgttc acacaaaaga cagcaaacat ccaattaaga 34140 ttatattttg actcttctgg aaatctatta actgaggaat cagacttaaa aattccactt 34200 aaaaataaat cttctacagc gaccagtgaa actgtagcca gcagcaaagc ctttatgcca 34260 agtactacag cttatccctt caacaccact actagggata gtgaaaacta cattcatgga 34320 atatgttact acatgactag ttatgataga agtctatttc ccttgaacat ttctataatg 34380 ctaaacagcc gtatgatttc ttccaatgtt gcctatgcca tacaatttga atggaatcta 34440 aatgcaagtg aatctccaga aagcaacata gctacgctga ccacatcccc ctttttcttt 34500 tcttacatta cagaagacga caactaaaga atcgtttgtg ttatgtttca acgtgtttat 34560 ttttcaattg cagaaaattt caagtcattt ttcattcagt agtatagccc caccaccaca 34620 tagcttatac agatcaccgt accttaatca aactcacaga accctagtat tcaacctgcc 34680 acctccctcc caacacacag agtacacagt cctttctccc cggctggcct taaaaagcat 34740 catatcatgg gtaacagaca tattcttagg tgttatattc cacacggttt cctgtcgagc 34800 caaacgctca tcagtgatat taataaactc cccgggcagc tcacttaagt tcatgtcgct 34860 gtccagctgc tgagccacag gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg 34920 agaagtccac gcctacatgg gggtagagtc ataatcgtgc atcaggatag ggcggtggtg 34980 ctgcagcagc gcgcgaataa actgctgccg ccgccgctcc gtcctgcagg aatacaacat 35040 ggcagtggtc tcctcagcga tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg 35100 ggcacagcag cgcaccctga tctcacttaa atcagcacag taactgcagc acagcaccac 35160 aatattgttc aaaatcccac agtgcaaggc gctgtatcca aagctcatgg cggggaccac 35220 agaacccacg tggccatcat accacaagcg caggtagatt aagtggcgac ccctcataaa 35280 cacgctggac ataaacatta cctcttttgg catgttgtaa ttcaccacct cccggtacca 35340 tataaacctc tgattaaaca tggcgccatc caccaccatc ctaaaccagc tggccaaaac 35400 ctgcccgccg gctatacact gcagggaacc gggactggaa caatgacagt ggagagccca 35460 ggactcgtaa ccatggatca tcatgctcgt catgatatca atgttggcac aacacaggca 35520 cacgtgcata cacttcctca ggattacaag ctcctcccgc gttagaacca tatcccaggg 35580 aacaacccat tcctgaatca gcgtaaatcc cacactgcag ggaagacctc gcacgtaact 35640 cacgttgtgc attgtcaaag tgttacattc gggcagcagc ggatgatcct ccagtatggt 35700 agcgcgggtt tctgtctcaa aaggaggtag acgatcccta ctgtacggag tgcgccgaga 35760 caaccgagat cgtgttggtc gtagtgtcat gccaaatgga acgccggacg tagtcatatt 35820 tcctgaagca aaaccaggtg cgggcgtgac aaacagatct gcgtctccgg tctcgccgct 35880 tagatcgctc tgtgtagtag ttgtagtata tccactctct caaagcatcc aggcgccccc 35940 tggcttcggg ttctatgtaa actccttcat gcgccgctgc cctgataaca tccaccaccg 36000 cagaataagc cacacccagc caacctacac attcgttctg cgagtcacac acgggaggag 36060 cgggaagagc tggaagaacc atgttttttt ttttattcca aaagattatc caaaacctca 36120 aaatgaagat ctattaagtg aacgcgctcc cctccggtgg cgtggtcaaa ctctacagcc 36180 aaagaacaga taatggcatt tgtaagatgt tgcacaatgg cttccaaaag gcaaacggcc 36240 ctcacgtcca agtggacgta aaggctaaac ccttcagggt gaatctcctc tataaacatt 36300 ccagcacctt caaccatgcc caaataattc tcatctcgcc accttctcaa tatatctcta 36360 agcaaatccc gaatattaag tccggccatt gtaaaaatct gctccagagc gccctccacc 36420 ttcagcctca agcagcgaat catgattgca aaaattcagg ttcctcacag acctgtataa 36480 gattcaaaag cggaacatta acaaaaatac cgcgatcccg taggtccctt cgcagggcca 36540 gctgaacata atcgtgcagg tctgcacgga ccagcgcggc cacttccccg ccaggaacca 36600 tgacaaaaga acccacactg attatgacac gcatactcgg agctatgcta accagcgtag 36660 ccccgatgta agcttgttgc atgggcggcg atataaaatg caaggtgctg ctcaaaaaat 36720 caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc 36780 aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg 36840 gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct 36900 tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc 36960 gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg 37020 agtcataatg taagactcgg taaacacatc aggttgattc acatcggtca gtgctaaaaa 37080 gcgaccgaaa tagcccgggg gaatacatac ccgcaggcgt agagacaaca ttacagcccc 37140 cataggaggt ataacaaaat taataggaga gaaaaacaca taaacacctg aaaaaccctc 37200 ctgcctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc 37260 agccataaca gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac 37320 acggcaccag ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat 37380 aggactaaaa aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg 37440 aacctacgcc cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt 37500 tttcccacgt tacgtcactt cccattttaa gaaaactaca attcccaaca catacaagtt 37560 actccgccct aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac 37620 tccaccccct cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatg 37678 <210> 23 <211> 34739 <212> DNA <213> Artificial Sequence <220> <223> #1 vector <400> 23 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct 960 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 1020 agacaccggg accgatccag cctccgattt gccaccatgt ttgtgttcct ggtgctgctg 1080 ccactggtgt ccagccagtg tgtgaacctg accaccagga cccaacttcc tcctgcctac 1140 accaactcct tcaccagggg agtctactac cctgacaagg tgttcaggtc ctctgtgctg 1200 cacagcaccc aggacctgtt cctgccattc ttcagcaatg tgacctggtt ccatgccatc 1260 catgtgtctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc attcaatgat 1320 ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat ttttggcacc 1380 accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa tgtggtgatt 1440 aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg gagtctacta ccacaagaac 1500 aacaagtcct ggatggagtc tgagttcagg gtctactcct ctgccaacaa ctgtaccttt 1560 gaatatgtga gccaaccatt cctgatggac ttggagggca agcagggcaa cttcaagaac 1620 ctgagggagt ttgtgttcaa gaacattgat ggctacttca agatttacag caaacacaca 1680 ccaatcaacc tggtgaggga cctgccacag ggcttctctg ccttggaacc actggtggac 1740 ctgccaattg gcatcaacat caccaggttc cagaccctgc tggctctgca caggtcctac 1800 ctgacacctg gagactcctc ctctggctgg acagcaggag cagcagccta ctatgtgggc 1860 tacctccaac caaggacctt cctgctgaaa tacaatgaga atggcaccat cacagatgct 1920 gtggactgtg ccctggaccc actgtctgag accaagtgta ccctgaaatc cttcacagtg 1980 gagaagggca tctaccagac cagcaacttc agggtccaac caacagagag cattgtgagg 2040 tttccaaaca tcaccaacct gtgtccattt ggagaggtgt tcaatgccac caggtttgcc 2100 tctgtctatg cctggaacag gaagaggatt agcaactgtg tggctgacta ctctgtgctc 2160 tacaactctg cctccttcag caccttcaag tgttatggag tgagcccaac caaactgaat 2220 gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga ttaggggaga tgaggtgaga 2280 cagattgccc ctggacaaac aggcaagatt gctgactaca actacaaact gcctgatgac 2340 ttcacaggct gtgtgattgc ctggaacagc aacaacctgg acagcaaggt gggaggcaac 2400 tacaactacc tctacagact gttcaggaag agcaacctga aaccatttga gagggacatc 2460 agcacagaga tttaccaggc tggcagcaca ccatgtaatg gagtggaggg cttcaactgt 2520 tactttccac tccaatccta tggcttccaa ccaaccaatg gagtgggcta ccaaccatac 2580 agggtggtgg tgctgtcctt tgaactgctc catgcccctg ccacagtgtg tggaccaaag 2640 aagagcacca acctggtgaa gaacaagtgt gtgaacttca acttcaatgg actgacaggc 2700 acaggagtgc tgacagagag caacaagaag ttcctgccat tccaacagtt tggcagggac 2760 attgctgaca ccacagatgc tgtgagggac ccacagacct tggagattct ggacatcaca 2820 ccatgttcct ttggaggagt gtctgtgatt acacctggca ccaacaccag caaccaggtg 2880 gctgtgctct accaggatgt gaactgtact gaggtgcctg tggctatcca tgctgaccaa 2940 cttacaccaa cctggagggt ctacagcaca ggcagcaatg tgttccagac cagggctggc 3000 tgtctgattg gagcagagca tgtgaacaac tcctatgagt gtgacatccc aattggagca 3060 ggcatctgtg cctcctacca gacccagacc aacagcccaa ggagggcaag gtctgtggca 3120 agccagagca tcattgccta cacaatgagt ctgggagcag agaactctgt ggcttacagc 3180 aacaacagca ttgccatccc aaccaacttc accatctctg tgaccacaga gattctgcct 3240 gtgagtatga ccaagacctc tgtggactgt acaatgtata tctgtggaga cagcacagag 3300 tgtagcaacc tgctgctcca atatggctcc ttctgtaccc aacttaacag ggctctgaca 3360 ggcattgctg tggaacagga caagaacacc caggaggtgt ttgcccaggt gaagcagatt 3420 tacaagacac ctccaatcaa ggactttgga ggcttcaact tcagccagat tctgcctgac 3480 ccaagcaagc caagcaagag gtccttcatt gaggacctgc tgttcaacaa ggtgaccctg 3540 gctgatgctg gcttcatcaa gcaatatgga gactgtctgg gagacattgc tgccagggac 3600 ctgatttgtg cccagaagtt caatggactg acagtgctgc ctccactgct gacagatgag 3660 atgattgccc aatacacctc tgccctgctg gctggcacca tcacctctgg ctggaccttt 3720 ggagcaggag cagccctcca aatcccattt gctatgcaga tggcttacag gttcaatggc 3780 attggagtga cccagaatgt gctctatgag aaccagaaac tgattgccaa ccagttcaac 3840 tctgccattg gcaagattca ggactccctg tccagcacag cctctgccct gggcaaactc 3900 caagatgtgg tgaaccagaa tgcccaggct ctgaacaccc tggtgaagca actttccagc 3960 aactttggag ccatctcctc tgtgctgaat gacatcctga gcagactgga caaggtggag 4020 gctgaggtcc agattgacag actgattaca ggcagactcc aatccctcca aacctatgtg 4080 acccaacaac ttatcagggc tgctgagatt agggcatctg ccaacctggc tgccaccaag 4140 atgagtgagt gtgtgctggg acaaagcaag agggtggact tctgtggcaa gggctaccac 4200 ctgatgagtt ttccacagtc tgcccctcat ggagtggtgt tcctgcatgt gacctatgtg 4260 cctgcccagg agaagaactt caccacagcc cctgccatct gccatgatgg caaggctcac 4320 tttccaaggg agggagtgtt tgtgagcaat ggcacccact ggtttgtgac ccagaggaac 4380 ttctatgaac cacagattat caccacagac aacacctttg tgtctggcaa ctgtgatgtg 4440 gtgattggca ttgtgaacaa cacagtctat gacccactcc aacctgaact ggactccttc 4500 aaggaggaac tggacaaata cttcaagaac cacaccagcc ctgatgtgga cctgggagac 4560 atctctggca tcaatgcctc tgtggtgaac atccagaagg agattgacag actgaatgag 4620 gtggctaaga acctgaatga gtccctgatt gacctccaag aactgggcaa atatgaacaa 4680 tacatcaagt ggccatgaaa attgatcata atcagccata ccacatttgt agaggtttta 4740 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 4800 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4860 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 4920 aatgtatctt aacgcggatc tgggcgtggt taagggtggg aaagaatata taaggtgggg 4980 gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc accaactcgt 5040 ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc 5100 gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta 5160 ccttgaccta cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt 5220 cagccgctgc agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg 5280 caagcagtgc agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac 5340 aattggattc tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc 5400 agcaggtttc tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa 5460 aaccagactc tgtttggatt tggatcaagc aagtgtcttg ctgtctttat ttaggggttt 5520 tgcgcgcgcg gtaggcccgg gaccagcggt ctcggtcgtt gagggtcctg tgtatttttt 5580 ccaggacgtg gtaaaggtga ctctggatgt tcagatacat gggcataagc ccgtctctgg 5640 ggtggaggta gcaccactgc agagcttcat gctgcggggt ggtgttgtag atgatccagt 5700 cgtagcagga gcgctgggcg tggtgcctaa aaatgtcttt cagtagcaag ctgattgcca 5760 ggggcaggcc cttggtgtaa gtgtttacaa agcggttaag ctgggatggg tgcatacgtg 5820 gggatatgag atgcatcttg gactgtattt ttaggttggc tatgttccca gccatatccc 5880 tccggggatt catgttgtgc agaaccacca gcacagtgta tccggtgcac ttgggaaatt 5940 tgtcatgtag cttagaagga aatgcgtgga agaacttgga gacgcccttg tgacctccaa 6000 gattttccat gcattcgtcc ataatgatgg caatgggccc acgggcggcg gcctgggcga 6060 agatatttct gggatcacta acgtcatagt tgtgttccag gatgagatcg tcataggcca 6120 tttttacaaa gcgcgggcgg agggtgccag actgcggtat aatggttcca tccggcccag 6180 gggcgtagtt accctcacag atttgcattt cccacgcttt gagttcagat ggggggatca 6240 tgtctacctg cggggcgatg aagaaaacgg tttccggggt aggggagatc agctgggaag 6300 aaagcaggtt cctgagcagc tgcgacttac cgcagccggt gggcccgtaa atcacaccta 6360 ttaccggctg caactggtag ttaagagagc tgcagctgcc gtcatccctg agcagggggg 6420 ccacttcgtt aagcatgtcc ctgactcgca tgttttccct gaccaaatcc gccagaaggc 6480 gctcgccgcc cagcgatagc agttcttgca aggaagcaaa gtttttcaac ggtttgagac 6540 cgtccgccgt aggcatgctt ttgagcgttt gaccaagcag ttccaggcgg tcccacagct 6600 cggtcacctg ctctacggca tctcgatcca gcatatctcc tcgtttcgcg ggttggggcg 6660 gctttcgctg tacggcagta gtcggtgctc gtccagacgg gccagggtca tgtctttcca 6720 cgggcgcagg gtcctcgtca gcgtagtctg ggtcacggtg aaggggtgcg ctccgggctg 6780 cgcgctggcc agggtgcgct tgaggctggt cctgctggtg ctgaagcgct gccggtcttc 6840 gccctgcgcg tcggccaggt agcatttgac catggtgtca tagtccagcc cctccgcggc 6900 gtggcccttg gcgcgcagct tgcccttgga ggaggcgccg cacgaggggc agtgcagact 6960 tttgagggcg tagagcttgg gcgcgagaaa taccgattcc ggggagtagg catccgcgcc 7020 gcaggccccg cagacggtct cgcattccac gagccaggtg agctctggcc gttcggggtc 7080 aaaaaccagg tttcccccat gctttttgat gcgtttctta cctctggttt ccatgagccg 7140 gtgtccacgc tcggtgacga aaaggctgtc cgtgtccccg tatacagact tgagaggcct 7200 gtcctcgagc ggtgttccgc ggtcctcctc gtatagaaac tcggaccact ctgagacaaa 7260 ggctcgcgtc caggccagca cgaaggaggc taagtgggag gggtagcggt cgttgtccac 7320 tagggggtcc actcgctcca gggtgtgaag acacatgtcg ccctcttcgg catcaaggaa 7380 ggtgattggt ttgtaggtgt aggccacgtg accgggtgtt cctgaagggg ggctataaaa 7440 gggggtgggg gcgcgttcgt cctcactctc ttccgcatcg ctgtctgcga gggccagctg 7500 ttggggtgag tactccctct gaaaagcggg catgacttct gcgctaagat tgtcagtttc 7560 caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga gggtggccgc 7620 atccatctgg tcagaaaaga caatcttttt gttgtcaagc ttggtggcaa acgacccgta 7680 gagggcgttg gacagcaact tggcgatgga gcgcagggtt tggtttttgt cgcgatcggc 7740 gcgctccttg gccgcgatgt ttagctgcac gtattcgcgc gcaacgcacc gccattcggg 7800 aaagacggtg gtgcgctcgt cgggcaccag gtgcacgcgc caaccgcggt tgtgcagggt 7860 gacaaggtca acgctggtgg ctacctctcc gcgtaggcgc tcgttggtcc agcagaggcg 7920 gccgcccttg cgcgagcaga atggcggtag ggggtctagc tgcgtctcgt ccggggggtc 7980 tgcgtccacg gtaaagaccc cgggcagcag gcgcgcgtcg aagtagtcta tcttgcatcc 8040 ttgcaagtct agcgcctgct gccatgcgcg ggcggcaagc gcgcgctcgt atgggttgag 8100 tgggggaccc catggcatgg ggtgggtgag cgcggaggcg tacatgccgc aaatgtcgta 8160 aacgtagagg ggctctctga gtattccaag atatgtaggg tagcatcttc caccgcggat 8220 gctggcgcgc acgtaatcgt atagttcgtg cgagggagcg aggaggtcgg gaccgaggtt 8280 gctacgggcg ggctgctctg ctcggaagac tatctgcctg aagatggcat gtgagttgga 8340 tgatatggtt ggacgctgga agacgttgaa gctggcgtct gtgagaccta ccgcgtcacg 8400 cacgaaggag gcgtaggagt cgcgcagctt gttgaccagc tcggcggtga cctgcacgtc 8460 tagggcgcag tagtccaggg tttccttgat gatgtcatac ttatcctgtc cctttttttt 8520 ccacagctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa 8580 cccgtcggcc tccgaacggt aagagcctag catgtagaac tggttgacgg cctggtaggc 8640 gcagcatccc ttttctacgg gtagcgcgta tgcctgcgcg gccttccgga gcgaggtgtg 8700 ggtgagcgca aaggtgtccc tgaccatgac tttgaggtac tggtatttga agtcagtgtc 8760 gtcgcatccg ccctgctccc agagcaaaaa gtccgtgcgc tttttggaac gcggatttgg 8820 cagggcgaag gtgacatcgt tgaagagtat ctttcccgcg cgaggcataa agttgcgtgt 8880 gatgcggaag ggtcccggca cctcggaacg gttgttaatt acctgggcgg cgagcacgat 8940 ctcgtcaaag ccgttgatgt tgtggcccac aatgtaaagt tccaagaagc gcgggatgcc 9000 cttgatggaa ggcaattttt taagttcctc gtaggtgagc tcttcagggg agctgagccc 9060 gtgctctgaa agggcccagt ctgcaagatg agggttggaa gcgacgaatg agctccacag 9120 gtcacgggcc attagcattt gcaggtggtc gcgaaaggtc ctaaactggc gacctatggc 9180 cattttttct ggggtgatgc agtagaaggt aagcgggtct tgttcccagc ggtcccatcc 9240 aaggttcgcg gctaggtctc gcgcggcagt cactagaggc tcatctccgc cgaacttcat 9300 gaccagcatg aagggcacga gctgcttccc aaaggccccc atccaagtat aggtctctac 9360 atcgtaggtg acaaagagac gctcggtgcg aggatgcgag ccgatcggga agaactggat 9420 ctcccgccac caattggagg agtggctatt gatgtggtga aagtagaagt ccctgcgacg 9480 ggccgaacac tcgtgctggc ttttgtaaaa acgtgcgcag tactggcagc ggtgcacggg 9540 ctgtacatcc tgcacgaggt tgacctgacg accgcgcaca aggaagcaga gtgggaattt 9600 gagcccctcg cctggcgggt ttggctggtg gtcttctact tcggctgctt gtccttgacc 9660 gtctggctgc tcgaggggag ttacggtgga tcggaccacc acgccgcgcg agcccaaagt 9720 ccagatgtcc gcgcgcggcg gtcggagctt gatgacaaca tcgcgcagat gggagctgtc 9780 catggtctgg agctcccgcg gcgtcaggtc aggcgggagc tcctgcaggt ttacctcgca 9840 tagacgggtc agggcgcggg ctagatccag gtgataccta atttccaggg gctggttggt 9900 ggcggcgtcg atggcttgca agaggccgca tccccgcggc gcgactacgg taccgcgcgg 9960 cgggcggtgg gccgcggggg tgtccttgga tgatgcatct aaaagcggtg acgcgggcga 10020 gcccccggag gtaggggggg ctccggaccc gccgggagag ggggcagggg cacgtcggcg 10080 ccgcgcgcgg gcaggagctg gtgctgcgcg cgtaggttgc tggcgaacgc gacgacgcgg 10140 cggttgatct cctgaatctg gcgcctctgc gtgaagacga cgggcccggt gagcttgaac 10200 ctgaaagaga gttcgacaga atcaatttcg gtgtcgttga cggcggcctg gcgcaaaatc 10260 tcctgcacgt ctcctgagtt gtcttgatag gcgatctcgg ccatgaactg ctcgatctct 10320 tcctcctgga gatctccgcg tccggctcgc tccacggtgg cggcgaggtc gttggaaatg 10380 cgggccatga gctgcgagaa ggcgttgagg cctccctcgt tccagacgcg gctgtagacc 10440 acgccccctt cggcatcgcg ggcgcgcatg accacctgcg cgagattgag ctccacgtgc 10500 cgggcgaaga cggcgtagtt tcgcaggcgc tgaaagaggt agttgagggt ggtggcggtg 10560 tgttctgcca cgaagaagta cataacccag cgtcgcaacg tggattcgtt gatatccccc 10620 aaggcctcaa ggcgctccat ggcctcgtag aagtccacgg cgaagttgaa aaactgggag 10680 ttgcgcgccg acacggttaa ctcctcctcc agaagacgga tgagctcggc gacagtgtcg 10740 cgcacctcgc gctcaaaggc tacaggggcc tcttcttctt cttcaatctc ctcttccata 10800 agggcctccc cttcttcttc ttctggcggc ggtgggggag gggggacacg gcggcgacga 10860 cggcgcaccg ggaggcggtc gacaaagcgc tcgatcatct ccccgcggcg acggcgcatg 10920 gtctcggtga cggcgcggcc gttctcgcgg gggcgcagtt ggaagacgcc gcccgtcatg 10980 tcccggttat gggttggcgg ggggctgcca tgcggcaggg atacggcgct aacgatgcat 11040 ctcaacaatt gttgtgtagg tactccgccg ccgagggacc tgagcgagtc cgcatcgacc 11100 ggatcggaaa acctctcgag aaaggcgtct aaccagtcac agtcgcaagg taggctgagc 11160 accgtggcgg gcggcagcgg gcggcggtcg gggttgtttc tggcggaggt gctgctgatg 11220 atgtaattaa agtaggcggt cttgagacgg cggatggtcg acagaagcac catgtccttg 11280 ggtccggcct gctgaatgcg caggcggtcg gccatgcccc aggcttcgtt ttgacatcgg 11340 cgcaggtctt tgtagtagtc ttgcatgagc ctttctaccg gcacttcttc ttctccttcc 11400 tcttgtcctg catctcttgc atctatcgct gcggcggcgg cggagtttgg ccgtaggtgg 11460 cgccctcttc ctcccatgcg tgtgaccccg aagcccctca tcggctgaag cagggctagg 11520 tcggcgacaa cgcgctcggc taatatggcc tgctgcacct gcgtgagggt agactggaag 11580 tcatccatgt ccacaaagcg gtggtatgcg cccgtgttga tggtgtaagt gcagttggcc 11640 ataacggacc agttaacggt ctggtgaccc ggctgcgaga gctcggtgta cctgagacgc 11700 gagtaagccc tcgagtcaaa tacgtagtcg ttgcaagtcc gcaccaggta ctggtatccc 11760 accaaaaagt gcggcggcgg ctggcggtag aggggccagc gtagggtggc cggggctccg 11820 ggggcgagat cttccaacat aaggcgatga tatccgtaga tgtacctgga catccaggtg 11880 atgccggcgg cggtggtgga ggcgcgcgga aagtcgcgga cgcggttcca gatgttgcgc 11940 agcggcaaaa agtgctccat ggtcgggacg ctctggccgg tcaggcgcgc gcaatcgttg 12000 acgctctagc gtgcaaaagg agagcctgta agcgggcact cttccgtggt ctggtggata 12060 aattcgcaag ggtatcatgg cggacgaccg gggttcgagc cccgtatccg gccgtccgcc 12120 gtgatccatg cggttaccgc ccgcgtgtcg aacccaggtg tgcgacgtca gacaacgggg 12180 gagtgctcct tttggcttcc ttccaggcgc ggcggctgct gcgctagctt ttttggccac 12240 tggccgcgcg cagcgtaagc ggttaggctg gaaagcgaaa gcattaagtg gctcgctccc 12300 tgtagccgga gggttatttt ccaagggttg agtcgcggga cccccggttc gagtctcgga 12360 ccggccggac tgcggcgaac gggggtttgc ctccccgtca tgcaagaccc cgcttgcaaa 12420 ttcctccgga aacagggacg agcccctttt ttgcttttcc cagatgcatc cggtgctgcg 12480 gcagatgcgc ccccctcctc agcagcggca agagcaagag cagcggcaga catgcagggc 12540 accctcccct cctcctaccg cgtcaggagg ggcgacatcc gcggttgacg cggcagcaga 12600 tggtgattac gaacccccgc ggcgccgggc ccggcactac ctggacttgg aggagggcga 12660 gggcctggcg cggctaggag cgccctctcc tgagcggcac ccaagggtgc agctgaagcg 12720 tgatacgcgt gaggcgtacg tgccgcggca gaacctgttt cgcgaccgcg agggagagga 12780 gcccgaggag atgcgggatc gaaagttcca cgcagggcgc gagctgcggc atggcctgaa 12840 tcgcgagcgg ttgctgcgcg aggaggactt tgagcccgac gcgcgaaccg ggattagtcc 12900 cgcgcgcgca cacgtggcgg ccgccgacct ggtaaccgca tacgagcaga cggtgaacca 12960 ggagattaac tttcaaaaaa gctttaacaa ccacgtgcgt acgcttgtgg cgcgcgagga 13020 ggtggctata ggactgatgc atctgtggga ctttgtaagc gcgctggagc aaaacccaaa 13080 tagcaagccg ctcatggcgc agctgttcct tatagtgcag cacagcaggg acaacgaggc 13140 attcagggat gcgctgctaa acatagtaga gcccgagggc cgctggctgc tcgatttgat 13200 aaacatcctg cagagcatag tggtgcagga gcgcagcttg agcctggctg acaaggtggc 13260 cgccatcaac tattccatgc ttagcctggg caagttttac gcccgcaaga tataccatac 13320 cccttacgtt cccatagaca aggaggtaaa gatcgagggg ttctacatgc gcatggcgct 13380 gaaggtgctt accttgagcg acgacctggg cgtttatcgc aacgagcgca tccacaaggc 13440 cgtgagcgtg agccggcggc gcgagctcag cgaccgcgag ctgatgcaca gcctgcaaag 13500 ggccctggct ggcacgggca gcggcgatag agaggccgag tcctactttg acgcgggcgc 13560 tgacctgcgc tgggccccaa gccgacgcgc cctggaggca gctggggccg gacctgggct 13620 ggcggtggca cccgcgcgcg ctggcaacgt cggcggcgtg gaggaatatg acgaggacga 13680 tgagtacgag ccagaggacg gcgagtacta agcggtgatg tttctgatca gatgatgcaa 13740 gacgcaacgg acccggcggt gcgggcggcg ctgcagagcc agccgtccgg ccttaactcc 13800 acggacgact ggcgccaggt catggaccgc atcatgtcgc tgactgcgcg caatcctgac 13860 gcgttccggc agcagccgca ggccaaccgg ctctccgcaa ttctggaagc ggtggtcccg 13920 gcgcgcgcaa accccacgca cgagaaggtg ctggcgatcg taaacgcgct ggccgaaaac 13980 agggccatcc ggcccgacga ggccggcctg gtctacgacg cgctgcttca gcgcgtggct 14040 cgttacaaca gcggcaacgt gcagaccaac ctggaccggc tggtggggga tgtgcgcgag 14100 gccgtggcgc agcgtgagcg cgcgcagcag cagggcaacc tgggctccat ggttgcacta 14160 aacgccttcc tgagtacaca gcccgccaac gtgccgcggg gacaggagga ctacaccaac 14220 tttgtgagcg cactgcggct aatggtgact gagacaccgc aaagtgaggt gtaccagtct 14280 gggccagact attttttcca gaccagtaga caaggcctgc agaccgtaaa cctgagccag 14340 gctttcaaaa acttgcaggg gctgtggggg gtgcgggctc ccacaggcga ccgcgcgacc 14400 gtgtctagct tgctgacgcc caactcgcgc ctgttgctgc tgctaatagc gcccttcacg 14460 gacagtggca gcgtgtcccg ggacacatac ctaggtcact tgctgacact gtaccgcgag 14520 gccataggtc aggcgcatgt ggacgagcat actttccagg agattacaag tgtcagccgc 14580 gcgctggggc aggaggacac gggcagcctg gaggcaaccc taaactacct gctgaccaac 14640 cggcggcaga agatcccctc gttgcacagt ttaaacagcg aggaggagcg cattttgcgc 14700 tacgtgcagc agagcgtgag ccttaacctg atgcgcgacg gggtaacgcc cagcgtggcg 14760 ctggacatga ccgcgcgcaa catggaaccg ggcatgtatg cctcaaaccg gccgtttatc 14820 aaccgcctaa tggactactt gcatcgcgcg gccgccgtga accccgagta tttcaccaat 14880 gccatcttga acccgcactg gctaccgccc cctggtttct acaccggggg attcgaggtg 14940 cccgagggta acgatggatt cctctgggac gacatagacg acagcgtgtt ttccccgcaa 15000 ccgcagaccc tgctagagtt gcaacagcgc gagcaggcag aggcggcgct gcgaaaggaa 15060 agcttccgca ggccaagcag cttgtccgat ctaggcgctg cggccccgcg gtcagatgct 15120 agtagcccat ttccaagctt gatagggtct cttaccagca ctcgcaccac ccgcccgcgc 15180 ctgctgggcg aggaggagta cctaaacaac tcgctgctgc agccgcagcg cgaaaaaaac 15240 ctgcctccgg catttcccaa caacgggata gagagcctag tggacaagat gagtagatgg 15300 aagacgtacg cgcaggagca cagggacgtg ccaggcccgc gcccgcccac ccgtcgtcaa 15360 aggcacgacc gtcagcgggg tctggtgtgg gaggacgatg actcggcaga cgacagcagc 15420 gtcctggatt tgggagggag tggcaacccg tttgcgcacc ttcgccccag gctggggaga 15480 atgttttaaa aaaaaaaaag catgatgcaa aataaaaaac tcaccaaggc catggcaccg 15540 agcgttggtt ttcttgtatt ccccttagta tgcggcgcgc ggcgatgtat gaggaaggtc 15600 ctcctccctc ctacgagagt gtggtgagcg cggcgccagt ggcggcggcg ctgggttctc 15660 ccttcgatgc tcccctggac ccgccgtttg tgcctccgcg gtacctgcgg cctaccgggg 15720 ggagaaacag catccgttac tctgagttgg cacccctatt cgacaccacc cgtgtgtacc 15780 tggtggacaa caagtcaacg gatgtggcat ccctgaacta ccagaacgac cacagcaact 15840 ttctgaccac ggtcattcaa aacaatgact acagcccggg ggaggcaagc acacagacca 15900 tcaatcttga cgaccggtcg cactggggcg gcgacctgaa aaccatcctg cataccaaca 15960 tgccaaatgt gaacgagttc atgtttacca ataagtttaa ggcgcgggtg atggtgtcgc 16020 gcttgcctac taaggacaat caggtggagc tgaaatacga gtgggtggag ttcacgctgc 16080 ccgagggcaa ctactccgag accatgacca tagaccttat gaacaacgcg atcgtggagc 16140 actacttgaa agtgggcaga cagaacgggg ttctggaaag cgacatcggg gtaaagtttg 16200 acacccgcaa cttcagactg gggtttgacc ccgtcactgg tcttgtcatg cctggggtat 16260 atacaaacga agccttccat ccagacatca ttttgctgcc aggatgcggg gtggacttca 16320 cccacagccg cctgagcaac ttgttgggca tccgcaagcg gcaacccttc caggagggct 16380 ttaggatcac ctacgatgat ctggagggtg gtaacattcc cgcactgttg gatgtggacg 16440 cctaccaggc gagcttgaaa gatgacaccg aacagggcgg gggtggcgca ggcggcagca 16500 acagcagtgg cagcggcgcg gaagagaact ccaacgcggc agccgcggca atgcagccgg 16560 tggaggacat gaacgatcat gccattcgcg gcgacacctt tgccacacgg gctgaggaga 16620 agcgcgctga ggccgaagca gcggccgaag ctgccgcccc cgctgcgcaa cccgaggtcg 16680 agaagcctca gaagaaaccg gtgatcaaac ccctgacaga ggacagcaag aaacgcagtt 16740 acaacctaat aagcaatgac agcaccttca cccagtaccg cagctggtac cttgcataca 16800 actacggcga ccctcagacc ggaatccgct catggaccct gctttgcact cctgacgtaa 16860 cctgcggctc ggagcaggtc tactggtcgt tgccagacat gatgcaagac cccgtgacct 16920 tccgctccac gcgccagatc agcaactttc cggtggtggg cgccgagctg ttgcccgtgc 16980 actccaagag cttctacaac gaccaggccg tctactccca actcatccgc cagtttacct 17040 ctctgaccca cgtgttcaat cgctttcccg agaaccagat tttggcgcgc ccgccagccc 17100 ccaccatcac caccgtcagt gaaaacgttc ctgctctcac agatcacggg acgctaccgc 17160 tgcgcaacag catcggagga gtccagcgag tgaccattac tgacgccaga cgccgcacct 17220 gcccctacgt ttacaaggcc ctgggcatag tctcgccgcg cgtcctatcg agccgcactt 17280 tttgagcaag catgtccatc cttatatcgc ccagcaataa cacaggctgg ggcctgcgct 17340 tcccaagcaa gatgtttggc ggggccaaga agcgctccga ccaacaccca gtgcgcgtgc 17400 gcgggcacta ccgcgcgccc tggggcgcgc acaaacgcgg ccgcactggg cgcaccaccg 17460 tcgatgacgc catcgacgcg gtggtggagg aggcgcgcaa ctacacgccc acgccgccac 17520 cagtgtccac agtggacgcg gccattcaga ccgtggtgcg cggagcccgg cgctatgcta 17580 aaatgaagag acggcggagg cgcgtagcac gtcgccaccg ccgccgaccc ggcactgccg 17640 cccaacgcgc ggcggcggcc ctgcttaacc gcgcacgtcg caccggccga cgggcggcca 17700 tgcgggccgc tcgaaggctg gccgcgggta ttgtcactgt gccccccagg tccaggcgac 17760 gagcggccgc cgcagcagcc gcggccatta gtgctatgac tcagggtcgc aggggcaacg 17820 tgtattgggt gcgcgactcg gttagcggcc tgcgcgtgcc cgtgcgcacc cgccccccgc 17880 gcaactagat tgcaagaaaa aactacttag actcgtactg ttgtatgtat ccagcggcgg 17940 cggcgcgcaa cgaagctatg tccaagcgca aaatcaaaga agagatgctc caggtcatcg 18000 cgccggagat ctatggcccc ccgaagaagg aagagcagga ttacaagccc cgaaagctaa 18060 agcgggtcaa aaagaaaaag aaagatgatg atgatgaact tgacgacgag gtggaactgc 18120 tgcacgctac cgcgcccagg cgacgggtac agtggaaagg tcgacgcgta aaacgtgttt 18180 tgcgacccgg caccaccgta gtctttacgc ccggtgagcg ctccacccgc acctacaagc 18240 gcgtgtatga tgaggtgtac ggcgacgagg acctgcttga gcaggccaac gagcgcctcg 18300 gggagtttgc ctacggaaag cggcataagg acatgctggc gttgccgctg gacgagggca 18360 acccaacacc tagcctaaag cccgtaacac tgcagcaggt gctgcccgcg cttgcaccgt 18420 ccgaagaaaa gcgcggccta aagcgcgagt ctggtgactt ggcacccacc gtgcagctga 18480 tggtacccaa gcgccagcga ctggaagatg tcttggaaaa aatgaccgtg gaacctgggc 18540 tggagcccga ggtccgcgtg cggccaatca agcaggtggc gccgggactg ggcgtgcaga 18600 ccgtggacgt tcagataccc actaccagta gcaccagtat tgccaccgcc acagagggca 18660 tggagacaca aacgtccccg gttgcctcag cggtggcgga tgccgcggtg caggcggtcg 18720 ctgcggccgc gtccaagacc tctacggagg tgcaaacgga cccgtggatg tttcgcgttt 18780 cagccccccg gcgcccgcgc cgttcgagga agtacggcgc cgccagcgcg ctactgcccg 18840 aatatgccct acatccttcc attgcgccta cccccggcta tcgtggctac acctaccgcc 18900 ccagaagacg agcaactacc cgacgccgaa ccaccactgg aacccgccgc cgccgtcgcc 18960 gtcgccagcc cgtgctggcc ccgatttccg tgcgcagggt ggctcgcgaa ggaggcagga 19020 ccctggtgct gccaacagcg cgctaccacc ccagcatcgt ttaaaagccg gtctttgtgg 19080 ttcttgcaga tatggccctc acctgccgcc tccgtttccc ggtgccggga ttccgaggaa 19140 gaatgcaccg taggaggggc atggccggcc acggcctgac gggcggcatg cgtcgtgcgc 19200 accaccggcg gcggcgcgcg tcgcaccgtc gcatgcgcgg cggtatcctg cccctcctta 19260 ttccactgat cgccgcggcg attggcgccg tgcccggaat tgcatccgtg gccttgcagg 19320 cgcagagaca ctgattaaaa acaagttgca tgtggaaaaa tcaaaataaa aagtctggac 19380 tctcacgctc gcttggtcct gtaactattt tgtagaatgg aagacatcaa ctttgcgtct 19440 ctggccccgc gacacggctc gcgcccgttc atgggaaact ggcaagatat cggcaccagc 19500 aatatgagcg gtggcgcctt cagctggggc tcgctgtgga gcggcattaa aaatttcggt 19560 tccaccgtta agaactatgg cagcaaggcc tggaacagca gcacaggcca gatgctgagg 19620 gataagttga aagagcaaaa tttccaacaa aaggtggtag atggcctggc ctctggcatt 19680 agcggggtgg tggacctggc caaccaggca gtgcaaaata agattaacag taagcttgat 19740 ccccgccctc ccgtagagga gcctccaccg gccgtggaga cagtgtctcc agaggggcgt 19800 ggcgaaaagc gtccgcgccc cgacagggaa gaaactctgg tgacgcaaat agacgagcct 19860 ccctcgtacg aggaggcact aaagcaaggc ctgcccacca cccgtcccat cgcgcccatg 19920 gctaccggag tgctgggcca gcacacaccc gtaacgctgg acctgcctcc ccccgccgac 19980 acccagcaga aacctgtgct gccaggcccg accgccgttg ttgtaacccg tcctagccgc 20040 gcgtccctgc gccgcgccgc cagcggtccg cgatcgttgc ggcccgtagc cagtggcaac 20100 tggcaaagca cactgaacag catcgtgggt ctgggggtgc aatccctgaa gcgccgacga 20160 tgcttctgat agctaacgtg tcgtatgtgt gtcatgtatg cgtccatgtc gccgccagag 20220 gagctgctga gccgccgcgc gcccgctttc caagatggct accccttcga tgatgccgca 20280 gtggtcttac atgcacatct cgggccagga cgcctcggag tacctgagcc ccgggctggt 20340 gcagtttgcc cgcgccaccg agacgtactt cagcctgaat aacaagttta gaaaccccac 20400 ggtggcgcct acgcacgacg tgaccacaga ccggtcccag cgtttgacgc tgcggttcat 20460 ccctgtggac cgtgaggata ctgcgtactc gtacaaggcg cggttcaccc tagctgtggg 20520 tgataaccgt gtgctggaca tggcttccac gtactttgac atccgcggcg tgctggacag 20580 gggccctact tttaagccct actctggcac tgcctacaac gccctggctc ccaagggtgc 20640 cccaaatcct tgcgaatggg atgaagctgc tactgctctt gaaataaacc tagaagaaga 20700 ggacgatgac aacgaagacg aagtagacga gcaagctgag cagcaaaaaa ctcacgtatt 20760 tgggcaggcg ccttattctg gtataaatat tacaaaggag ggtattcaaa taggtgtcga 20820 aggtcaaaca cctaaatatg ccgataaaac atttcaacct gaacctcaaa taggagaatc 20880 tcagtggtac gaaacagaaa ttaatcatgc agctgggaga gtcctaaaaa agactacccc 20940 aatgaaacca tgttacggtt catatgcaaa acccacaaat gaaaatggag ggcaaggcat 21000 tcttgtaaag caacaaaatg gaaagctaga aagtcaagtg gaaatgcaat ttttctcaac 21060 tactgaggca gccgcaggca atggtgataa cttgactcct aaagtggtat tgtacagtga 21120 agatgtagat atagaaaccc cagacactca tatttcttac atgcccacta ttaaggaagg 21180 taactcacga gaactaatgg gccaacaatc tatgcccaac aggcctaatt acattgcttt 21240 tagggacaat tttattggtc taatgtatta caacagcacg ggtaatatgg gtgttctggc 21300 gggccaagca tcgcagttga atgctgttgt agatttgcaa gacagaaaca cagagctttc 21360 ataccagctt ttgcttgatt ccattggtga tagaaccagg tacttttcta tgtggaatca 21420 ggctgttgac agctatgatc cagatgttag aattattgaa aatcatggaa ctgaagatga 21480 acttccaaat tactgctttc cactgggagg tgtgattaat acagagactc ttaccaaggt 21540 aaaacctaaa acaggtcagg aaaatggatg ggaaaaagat gctacagaat tttcagataa 21600 aaatgaaata agagttggaa ataattttgc catggaaatc aatctaaatg ccaacctgtg 21660 gagaaatttc ctgtactcca acatagcgct gtatttgccc gacaagctaa agtacagtcc 21720 ttccaacgta aaaatttctg ataacccaaa cacctacgac tacatgaaca agcgagtggt 21780 ggctcccggg ctagtggact gctacattaa ccttggagca cgctggtccc ttgactatat 21840 ggacaacgtc aacccattta accaccaccg caatgctggc ctgcgctacc gctcaatgtt 21900 gctgggcaat ggtcgctatg tgcccttcca catccaggtg cctcagaagt tctttgccat 21960 taaaaacctc cttctcctgc cgggctcata cacctacgag tggaacttca ggaaggatgt 22020 taacatggtt ctgcagagct ccctaggaaa tgacctaagg gttgacggag ccagcattaa 22080 gtttgatagc atttgccttt acgccacctt cttccccatg gcccacaaca ccgcctccac 22140 gcttgaggcc atgcttagaa acgacaccaa cgaccagtcc tttaacgact atctctccgc 22200 cgccaacatg ctctacccta tacccgccaa cgctaccaac gtgcccatat ccatcccctc 22260 ccgcaactgg gcggctttcc gcggctgggc cttcacgcgc cttaagacta aggaaacccc 22320 atcactgggc tcgggctacg acccttatta cacctactct ggctctatac cctacctaga 22380 tggaaccttt tacctcaacc acacctttaa gaaggtggcc attacctttg actcttctgt 22440 cagctggcct ggcaatgacc gcctgcttac ccccaacgag tttgaaatta agcgctcagt 22500 tgacggggag ggttacaacg ttgcccagtg taacatgacc aaagactggt tcctggtaca 22560 aatgctagct aactataaca ttggctacca gggcttctat atcccagaga gctacaagga 22620 ccgcatgtac tccttcttta gaaacttcca gcccatgagc cgtcaggtgg tggatgatac 22680 taaatacaag gactaccaac aggtgggcat cctacaccaa cacaacaact ctggatttgt 22740 tggctacctt gcccccacca tgcgcgaagg acaggcctac cctgctaact tcccctatcc 22800 gcttataggc aagaccgcag ttgacagcat tacccagaaa aagtttcttt gcgatcgcac 22860 cctttggcgc atcccattct ccagtaactt tatgtccatg ggcgcactca cagacctggg 22920 ccaaaacctt ctctacgcca actccgccca cgcgctagac atgacttttg aggtggatcc 22980 catggacgag cccacccttc tttatgtttt gtttgaagtc tttgacgtgg tccgtgtgca 23040 ccagccgcac cgcggcgtca tcgaaaccgt gtacctgcgc acgcccttct cggccggcaa 23100 cgccacaaca taaagaagca agcaacatca acaacagctg ccgccatggg ctccagtgag 23160 caggaactga aagccattgt caaagatctt ggttgtgggc catatttttt gggcacctat 23220 gacaagcgct ttccaggctt tgtttctcca cacaagctcg cctgcgccat agtcaatacg 23280 gccggtcgcg agactggggg cgtacactgg atggcctttg cctggaaccc gcactcaaaa 23340 acatgctacc tctttgagcc ctttggcttt tctgaccagc gactcaagca ggtttaccag 23400 tttgagtacg agtcactcct gcgccgtagc gccattgctt cttcccccga ccgctgtata 23460 acgctggaaa agtccaccca aagcgtacag gggcccaact cggccgcctg tggactattc 23520 tgctgcatgt ttctccacgc ctttgccaac tggccccaaa ctcccatgga tcacaacccc 23580 accatgaacc ttattaccgg ggtacccaac tccatgctca acagtcccca ggtacagccc 23640 accctgcgtc gcaaccagga acagctctac agcttcctgg agcgccactc gccctacttc 23700 cgcagccaca gtgcgcagat taggagcgcc acttcttttt gtcacttgaa aaacatgtaa 23760 aaataatgta ctagagacac tttcaataaa ggcaaatgct tttatttgta cactctcggg 23820 tgattattta cccccaccct tgccgtctgc gccgtttaaa aatcaaaggg gttctgccgc 23880 gcatcgctat gcgccactgg cagggacacg ttgcgatact ggtgtttagt gctccactta 23940 aactcaggca caaccatccg cggcagctcg gtgaagtttt cactccacag gctgcgcacc 24000 atcaccaacg cgtttagcag gtcgggcgcc gatatcttga agtcgcagtt ggggcctccg 24060 ccctgcgcgc gcgagttgcg atacacaggg ttgcagcact ggaacactat cagcgccggg 24120 tggtgcacgc tggccagcac gctcttgtcg gagatcagat ccgcgtccag gtcctccgcg 24180 ttgctcaggg cgaacggagt caactttggt agctgccttc ccaaaaaggg cgcgtgccca 24240 ggctttgagt tgcactcgca ccgtagtggc atcaaaaggt gaccgtgccc ggtctgggcg 24300 ttaggataca gcgcctgcat aaaagccttg atctgcttaa aagccacctg agcctttgcg 24360 ccttcagaga agaacatgcc gcaagacttg ccggaaaact gattggccgg acaggccgcg 24420 tcgtgcacgc agcaccttgc gtcggtgttg gagatctgca ccacatttcg gccccaccgg 24480 ttcttcacga tcttggcctt gctagactgc tccttcagcg cgcgctgccc gttttcgctc 24540 gtcacatcca tttcaatcac gtgctcctta tttatcataa tgcttccgtg tagacactta 24600 agctcgcctt cgatctcagc gcagcggtgc agccacaacg cgcagcccgt gggctcgtga 24660 tgcttgtagg tcacctctgc aaacgactgc aggtacgcct gcaggaatcg ccccatcatc 24720 gtcacaaagg tcttgttgct ggtgaaggtc agctgcaacc cgcggtgctc ctcgttcagc 24780 caggtcttgc atacggccgc cagagcttcc acttggtcag gcagtagttt gaagttcgcc 24840 tttagatcgt tatccacgtg gtacttgtcc atcagcgcgc gcgcagcctc catgcccttc 24900 tcccacgcag acacgatcgg cacactcagc gggttcatca ccgtaatttc actttccgct 24960 tcgctgggct cttcctcttc ctcttgcgtc cgcataccac gcgccactgg gtcgtcttca 25020 ttcagccgcc gcactgtgcg cttacctcct ttgccatgct tgattagcac cggtgggttg 25080 ctgaaaccca ccatttgtag cgccacatct tctctttctt cctcgctgtc cacgattacc 25140 tctggtgatg gcgggcgctc gggcttggga gaagggcgct tctttttctt cttgggcgca 25200 atggccaaat ccgccgccga ggtcgatggc cgcgggctgg gtgtgcgcgg caccagcgcg 25260 tcttgtgatg agtcttcctc gtcctcggac tcgatacgcc gcctcatccg cttttttggg 25320 ggcgcccggg gaggcggcgg cgacggggac ggggacgaca cgtcctccat ggttggggga 25380 cgtcgcgccg caccgcgtcc gcgctcgggg gtggtttcgc gctgctcctc ttcccgactg 25440 gccatttcct tctcctatag gcagaaaaag atcatggagt cagtcgagaa gaaggacagc 25500 ctaaccgccc cctctgagtt cgccaccacc gcctccaccg atgccgccaa cgcgcctacc 25560 accttccccg tcgaggcacc cccgcttgag gaggaggaag tgattatcga gcaggaccca 25620 ggttttgtaa gcgaagacga cgaggaccgc tcagtaccaa cagaggataa aaagcaagac 25680 caggacaacg cagaggcaaa cgaggaacaa gtcgggcggg gggacgaaag gcatggcgac 25740 tacctagatg tgggagacga cgtgctgttg aagcatctgc agcgccagtg cgccattatc 25800 tgcgacgcgt tgcaagagcg cagcgatgtg cccctcgcca tagcggatgt cagccttgcc 25860 tacgaacgcc acctattctc accgcgcgta ccccccaaac gccaagaaaa cggcacatgc 25920 gagcccaacc cgcgcctcaa cttctacccc gtatttgccg tgccagaggt gcttgccacc 25980 tatcacatct ttttccaaaa ctgcaagata cccctatcct gccgtgccaa ccgcagccga 26040 gcggacaagc agctggcctt gcggcagggc gctgtcatac ctgatatcgc ctcgctcaac 26100 gaagtgccaa aaatctttga gggtcttgga cgcgacgaga agcgcgcggc aaacgctctg 26160 caacaggaaa acagcgaaaa tgaaagtcac tctggagtgt tggtggaact cgagggtgac 26220 aacgcgcgcc tagccgtact aaaacgcagc atcgaggtca cccactttgc ctacccggca 26280 cttaacctac cccccaaggt catgagcaca gtcatgagtg agctgatcgt gcgccgtgcg 26340 cagcccctgg agagggatgc aaatttgcaa gaacaaacag aggagggcct acccgcagtt 26400 ggcgacgagc agctagcgcg ctggcttcaa acgcgcgagc ctgccgactt ggaggagcga 26460 cgcaaactaa tgatggccgc agtgctcgtt accgtggagc ttgagtgcat gcagcggttc 26520 tttgctgacc cggagatgca gcgcaagcta gaggaaacat tgcactacac ctttcgacag 26580 ggctacgtac gccaggcctg caagatctcc aacgtggagc tctgcaacct ggtctcctac 26640 cttggaattt tgcacgaaaa ccgccttggg caaaacgtgc ttcattccac gctcaagggc 26700 gaggcgcgcc gcgactacgt ccgcgactgc gtttacttat ttctatgcta cacctggcag 26760 acggccatgg gcgtttggca gcagtgcttg gaggagtgca acctcaagga gctgcagaaa 26820 ctgctaaagc aaaacttgaa ggacctatgg acggccttca acgagcgctc cgtggccgcg 26880 cacctggcgg acatcatttt ccccgaacgc ctgcttaaaa ccctgcaaca gggtctgcca 26940 gacttcacca gtcaaagcat gttgcagaac tttaggaact ttatcctaga gcgctcagga 27000 atcttgcccg ccacctgctg tgcacttcct agcgactttg tgcccattaa gtaccgcgaa 27060 tgccctccgc cgctttgggg ccactgctac cttctgcagc tagccaacta ccttgcctac 27120 cactctgaca taatggaaga cgtgagcggt gacggtctac tggagtgtca ctgtcgctgc 27180 aacctatgca ccccgcaccg ctccctggtt tgcaattcgc agctgcttaa cgaaagtcaa 27240 attatcggta cctttgagct gcagggtccc tcgcctgacg aaaagtccgc ggctccgggg 27300 ttgaaactca ctccggggct gtggacgtcg gcttaccttc gcaaatttgt acctgaggac 27360 taccacgccc acgagattag gttctacgaa gaccaatccc gcccgcctaa tgcggagctt 27420 accgcctgcg tcattaccca gggccacatt cttggccaat tgcaagccat caacaaagcc 27480 cgccaagagt ttctgctacg aaagggacgg ggggtttact tggaccccca gtccggcgag 27540 gagctcaacc caatcccccc gccgccgcag ccctatcagc agcagccgcg ggcccttgct 27600 tcccaggatg gcacccaaaa agaagctgca gctgccgccg ccacccacgg acgaggagga 27660 atactgggac agtcaggcag aggaggtttt ggacgaggag gaggaggaca tgatggaaga 27720 ctgggagagc ctagacgagg aagcttccga ggtcgaagag gtgtcagacg aaacaccgtc 27780 accctcggtc gcattcccct cgccggcgcc ccagaaatcg gcaaccggtt ccagcatggc 27840 tacaacctcc gctcctcagg cgccgccggc actgcccgtt cgccgaccca accgtagatg 27900 ggacaccact ggaaccaggg ccggtaagtc caagcagccg ccgccgttag cccaagagca 27960 acaacagcgc caaggctacc gctcatggcg cgggcacaag aacgccatag ttgcttgctt 28020 gcaagactgt gggggcaaca tctccttcgc ccgccgcttt cttctctacc atcacggcgt 28080 ggccttcccc cgtaacatcc tgcattacta ccgtcatctc tacagcccat actgcaccgg 28140 cggcagcggc agcaacagca gcggccacac agaagcaaag gcgaccggat agcaagactc 28200 tgacaaagcc caagaaatcc acagcggcgg cagcagcagg aggaggagcg ctgcgtctgg 28260 cgcccaacga acccgtatcg acccgcgagc ttagaaacag gatttttccc actctgtatg 28320 ctatatttca acagagcagg ggccaagaac aagagctgaa aataaaaaac aggtctctgc 28380 gatccctcac ccgcagctgc ctgtatcaca aaagcgaaga tcagcttcgg cgcacgctgg 28440 aagacgcgga ggctctcttc agtaaatact gcgcgctgac tcttaaggac tagtttcgcg 28500 ccctttctca aatttaagcg cgaaaactac gtcatctcca gcggccacac ccggcgccag 28560 cacctgttgt cagcgccatt atgagcaagg aaattcccac gccctacatg tggagttacc 28620 agccacaaat gggacttgcg gctggagctg cccaagacta ctcaacccga ataaactaca 28680 tgagcgcggg accccacatg atatcccggg tcaacggaat acgcgcccac cgaaaccgaa 28740 ttctcctgga acaggcggct attaccacca cacctcgtaa taaccttaat ccccgtagtt 28800 ggcccgctgc cctggtgtac caggaaagtc ccgctcccac cactgtggta cttcccagag 28860 acgcccaggc cgaagttcag atgactaact caggggcgca gcttgcgggc ggctttcgtc 28920 acagggtgcg gtcgcccggg cagggtataa ctcacctgac aatcagaggg cgaggtattc 28980 agctcaacga cgagtcggtg agctcctcgc ttggtctccg tccggacggg acatttcaga 29040 tcggcggcgc cggccgctct tcattcacgc ctcgtcaggc aatcctaact ctgcagacct 29100 cgtcctctga gccgcgctct ggaggcattg gaactctgca atttattgag gagtttgtgc 29160 catcggtcta ctttaacccc ttctcgggac ctcccggcca ctatccggat caatttattc 29220 ctaactttga cgcggtaaag gactcggcgg acggctacga ctgaatgtta agtggagagg 29280 cagagcaact gcgcctgaaa cacctggtcc actgtcgccg ccacaagtgc tttgcccgcg 29340 actccggtga gttttgctac tttgaattgc ccgaggatca tatcgagggc ccggcgcacg 29400 gcgtccggct taccgcccag ggagagcttg cccgtagcct gattcgggag tttacccagc 29460 gccccctgct agttgagcgg gacaggggac cctgtgttct cactgtgatt tgcaactgtc 29520 ctaaccctgg attacatcaa gatcctctag ttaatgtcag gtcgcctaag tcgattaact 29580 agagtacccg gggatcttat tccctttaac taataaaaaa aaataataaa gcatcactta 29640 cttaaaatca gttagcaaat ttctgtccag tttattcagc agcacctcct tgccctcctc 29700 ccagctctgg tattgcagct tcctcctggc tgcaaacttt ctccacaatc taaatggaat 29760 gtcagtttcc tcctgttcct gtccatccgc acccactatc ttcatgttgt tgcagatgaa 29820 gcgcgcaaga ccgtctgaag ataccttcaa ccccgtgtat ccatatgaca cggaaaccgg 29880 tcctccaact gtgccttttc ttactcctcc ctttgtatcc cccaatgggt ttcaagagag 29940 tccccctggg gtactctctt tgcgcctatc cgaacctcta gttacctcca atggcatgct 30000 tgcgctcaaa atgggcaacg gcctctctct ggacgaggcc ggcaacctta cctcccaaaa 30060 tgtaaccact gtgagcccac ctctcaaaaa aaccaagtca aacataaacc tggaaatatc 30120 tgcacccctc acagttacct cagaagccct aactgtggct gccgccgcac ctctaatggt 30180 cgcgggcaac acactcacca tgcaatcaca ggccccgcta accgtgcacg actccaaact 30240 tagcattgcc acccaaggac ccctcacagt gtcagaagga aagctagccc tgcaaacatc 30300 aggccccctc accaccaccg atagcagtac ccttactatc actgcctcac cccctctaac 30360 tactgccact ggtagcttgg gcattgactt gaaagagccc atttatacac aaaatggaaa 30420 actaggacta aagtacgggg ctcctttgca tgtaacagac gacctaaaca ctttgaccgt 30480 agcaactggt ccaggtgtga ctattaataa tacttccttg caaactaaag ttactggagc 30540 cttgggtttt gattcacaag gcaatatgca acttaatgta gcaggaggac taaggattga 30600 ttctcaaaac agacgcctta tacttgatgt tagttatccg tttgatgctc aaaaccaact 30660 aaatctaaga ctaggacagg gccctctttt tataaactca gcccacaact tggatattaa 30720 ctacaacaaa ggcctttact tgtttacagc ttcaaacaat tccaaaaagc ttgaggttaa 30780 cctaagcact gccaaggggt tgatgtttga cgctacagcc atagccatta atgcaggaga 30840 tgggcttgaa tttggttcac ctaatgcacc aaacacaaat cccctcaaaa caaaaattgg 30900 ccatggccta gaatttgatt caaacaaggc tatggttcct aaactaggaa ctggccttag 30960 ttttgacagc acaggtgcca ttacagtagg aaacaaaaat aatgataagc taactttgtg 31020 gaccggaata aaccctccac ctaactgtca aattgtggaa aacactaata caaatgatgg 31080 caaacttact ttagtattag taaaaaatgg agggcttgtt aatggctacg tgtctctagt 31140 tggtgtatca gacactgtga accaaatgtt cacacaaaag acagcaaaca tccaattaag 31200 attatatttt gactcttctg gaaatctatt aactgaggaa tcagacttaa aaattccact 31260 taaaaataaa tcttctacag cgaccagtga aactgtagcc agcagcaaag cctttatgcc 31320 aagtactaca gcttatccct tcaacaccac tactagggat agtgaaaact acattcatgg 31380 aatatgttac tacatgacta gttatgatag aagtctattt cccttgaaca tttctataat 31440 gctaaacagc cgtatgattt cttccaatgt tgcctatgcc atacaatttg aatggaatct 31500 aaatgcaagt gaatctccag aaagcaacat agctacgctg accacatccc cctttttctt 31560 ttcttacatt acagaagacg acaactaaag aatcgtttgt gttatgtttc aacgtgttta 31620 tttttcaatt gcagaaaatt tcaagtcatt tttcattcag tagtatagcc ccaccaccac 31680 atagcttata cagatcaccg taccttaatc aaactcacag aaccctagta ttcaacctgc 31740 cacctccctc ccaacacaca gagtacacag tcctttctcc ccggctggcc ttaaaaagca 31800 tcatatcatg ggtaacagac atattcttag gtgttatatt ccacacggtt tcctgtcgag 31860 ccaaacgctc atcagtgata ttaataaact ccccgggcag ctcacttaag ttcatgtcgc 31920 tgtccagctg ctgagccaca ggctgctgtc caacttgcgg ttgcttaacg ggcggcgaag 31980 gagaagtcca cgcctacatg ggggtagagt cataatcgtg catcaggata gggcggtggt 32040 gctgcagcag cgcgcgaata aactgctgcc gccgccgctc cgtcctgcag gaatacaaca 32100 tggcagtggt ctcctcagcg atgattcgca ccgcccgcag cataaggcgc cttgtcctcc 32160 gggcacagca gcgcaccctg atctcactta aatcagcaca gtaactgcag cacagcacca 32220 caatattgtt caaaatccca cagtgcaagg cgctgtatcc aaagctcatg gcggggacca 32280 cagaacccac gtggccatca taccacaagc gcaggtagat taagtggcga cccctcataa 32340 acacgctgga cataaacatt acctcttttg gcatgttgta attcaccacc tcccggtacc 32400 atataaacct ctgattaaac atggcgccat ccaccaccat cctaaaccag ctggccaaaa 32460 cctgcccgcc ggctatacac tgcagggaac cgggactgga acaatgacag tggagagccc 32520 aggactcgta accatggatc atcatgctcg tcatgatatc aatgttggca caacacaggc 32580 acacgtgcat acacttcctc aggattacaa gctcctcccg cgttagaacc atatcccagg 32640 gaacaaccca ttcctgaatc agcgtaaatc ccacactgca gggaagacct cgcacgtaac 32700 tcacgttgtg cattgtcaaa gtgttacatt cgggcagcag cggatgatcc tccagtatgg 32760 tagcgcgggt ttctgtctca aaaggaggta gacgatccct actgtacgga gtgcgccgag 32820 acaaccgaga tcgtgttggt cgtagtgtca tgccaaatgg aacgccggac gtagtcatat 32880 ttcctgaagc aaaaccaggt gcgggcgtga caaacagatc tgcgtctccg gtctcgccgc 32940 ttagatcgct ctgtgtagta gttgtagtat atccactctc tcaaagcatc caggcgcccc 33000 ctggcttcgg gttctatgta aactccttca tgcgccgctg ccctgataac atccaccacc 33060 gcagaataag ccacacccag ccaacctaca cattcgttct gcgagtcaca cacgggagga 33120 gcgggaagag ctggaagaac catgtttttt tttttattcc aaaagattat ccaaaacctc 33180 aaaatgaaga tctattaagt gaacgcgctc ccctccggtg gcgtggtcaa actctacagc 33240 caaagaacag ataatggcat ttgtaagatg ttgcacaatg gcttccaaaa ggcaaacggc 33300 cctcacgtcc aagtggacgt aaaggctaaa cccttcaggg tgaatctcct ctataaacat 33360 tccagcacct tcaaccatgc ccaaataatt ctcatctcgc caccttctca atatatctct 33420 aagcaaatcc cgaatattaa gtccggccat tgtaaaaatc tgctccagag cgccctccac 33480 cttcagcctc aagcagcgaa tcatgattgc aaaaattcag gttcctcaca gacctgtata 33540 agattcaaaa gcggaacatt aacaaaaata ccgcgatccc gtaggtccct tcgcagggcc 33600 agctgaacat aatcgtgcag gtctgcacgg accagcgcgg ccacttcccc gccaggaacc 33660 atgacaaaag aacccacact gattatgaca cgcatactcg gagctatgct aaccagcgta 33720 gccccgatgt aagcttgttg catgggcggc gatataaaat gcaaggtgct gctcaaaaaa 33780 tcaggcaaag cctcgcgcaa aaaagaaagc acatcgtagt catgctcatg cagataaagg 33840 caggtaagct ccggaaccac cacagaaaaa gacaccattt ttctctcaaa catgtctgcg 33900 ggtttctgca taaacacaaa ataaaataac aaaaaaacat ttaaacatta gaagcctgtc 33960 ttacaacagg aaaaacaacc cttataagca taagacggac tacggccatg ccggcgtgac 34020 cgtaaaaaaa ctggtcaccg tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg 34080 gagtcataat gtaagactcg gtaaacacat caggttgatt cacatcggtc agtgctaaaa 34140 agcgaccgaa atagcccggg ggaatacata cccgcaggcg tagagacaac attacagccc 34200 ccataggagg tataacaaaa ttaataggag agaaaaacac ataaacacct gaaaaaccct 34260 cctgcctagg caaaatagca ccctcccgct ccagaacaac atacagcgct tccacagcgg 34320 cagccataac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga 34380 cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata 34440 taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc 34500 gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg 34560 ttttcccacg ttacgtcact tcccatttta agaaaactac aattcccaac acatacaagt 34620 tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa 34680 ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatg 34739 <210> 24 <211> 37666 <212> DNA <213> Artificial Sequence <220> <223> plasmid #2 vector <400> 24 ttaattaaca tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 60 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 120 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 180 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 240 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 300 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 360 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 420 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 480 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 540 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 600 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 660 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 720 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 780 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 840 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 900 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 960 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1020 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1080 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1140 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1200 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1260 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1320 ttgttgccat tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca 1380 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 1440 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 1500 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 1560 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 1620 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 1680 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1740 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1800 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1860 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1920 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1980 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2040 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2100 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2160 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 2220 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2280 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2340 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2400 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2460 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 2520 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2580 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 2640 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 2700 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 2760 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 2820 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2880 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 2940 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 3000 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 3060 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 3120 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 3180 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 3240 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta 3300 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 3360 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 3420 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 3480 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 3540 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 3600 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 3660 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 3720 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3780 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3840 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 3900 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 3960 gacaccggga ccgatccagc ctccgatttg ccaccatgtt tgtgttcctg gtgctgctgc 4020 cactggtgtc cagccagtgt gtgaacctga ccaccaggac ccaacttcct cctgcctaca 4080 ccaactcctt caccagggga gtctactacc ctgacaaggt gttcaggtcc tctgtgctgc 4140 acagcaccca ggacctgttc ctgccattct tcagcaatgt gacctggttc catgccatcc 4200 atgtgtctgg caccaatggc accaagaggt ttgacaaccc tgtgctgcca ttcaatgatg 4260 gagtctactt tgccagcaca gagaagagca acatcatcag gggctggatt tttggcacca 4320 ccctggacag caagacccag tccctgctga ttgtgaacaa tgccaccaat gtggtgatta 4380 aggtgtgtga gttccagttc tgtaatgacc cattcctggg agtctactac cacaagaaca 4440 acaagtcctg gatggagtct gagttcaggg tctactcctc tgccaacaac tgtacctttg 4500 aatatgtgag ccaaccattc ctgatggact tggagggcaa gcagggcaac ttcaagaacc 4560 tgagggagtt tgtgttcaag aacattgatg gctacttcaa gatttacagc aaacacacac 4620 caatcaacct ggtgagggac ctgccacagg gcttctctgc cttggaacca ctggtggacc 4680 tgccaattgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcctacc 4740 tgacacctgg agactcctcc tctggctgga cagcaggagc agcagcctac tatgtgggct 4800 acctccaacc aaggaccttc ctgctgaaat acaatgagaa tggcaccatc acagatgctg 4860 tggactgtgc cctggaccca ctgtctgaga ccaagtgtac cctgaaatcc ttcacagtgg 4920 agaagggcat ctaccagacc agcaacttca gggtccaacc aacagagagc attgtgaggt 4980 ttccaaacat caccaacctg tgtccatttg gagaggtgtt caatgccacc aggtttgcct 5040 ctgtctatgc ctggaacagg aagaggatta gcaactgtgt ggctgactac tctgtgctct 5100 acaactctgc ctccttcagc accttcaagt gttatggagt gagcccaacc aaactgaatg 5160 acctgtgttt caccaatgtc tatgctgact cctttgtgat taggggagat gaggtgagac 5220 agattgcccc tggacaaaca ggcaagattg ctgactacaa ctacaaactg cctgatgact 5280 tcacaggctg tgtgattgcc tggaacagca acaacctgga cagcaaggtg ggaggcaact 5340 acaactacct ctacagactg ttcaggaaga gcaacctgaa accatttgag agggacatca 5400 gcacagagat ttaccaggct ggcagcacac catgtaatgg agtggagggc ttcaactgtt 5460 actttccact ccaatcctat ggcttccaac caaccaatgg agtgggctac caaccataca 5520 gggtggtggt gctgtccttt gaactgctcc atgcccctgc cacagtgtgt ggaccaaaga 5580 agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa cttcaatgga ctgacaggca 5640 caggagtgct gacagagagc aacaagaagt tcctgccatt ccaacagttt ggcagggaca 5700 ttgctgacac cacagatgct gtgagggacc cacagacctt ggagattctg gacatcacac 5760 catgttcctt tggaggagtg tctgtgatta cacctggcac caacaccagc aaccaggtgg 5820 ctgtgctcta ccaggatgtg aactgtactg aggtgcctgt ggctatccat gctgaccaac 5880 ttacaccaac ctggagggtc tacagcacag gcagcaatgt gttccagacc agggctggct 5940 gtctgattgg agcagagcat gtgaacaact cctatgagtg tgacatccca attggagcag 6000 gcatctgtgc ctcctaccag acccagacca tcctcaggtc tgtggcaagc cagagcatca 6060 ttgcctacac aatgagtctg ggagcagaga actctgtggc ttacagcaac aacagcattg 6120 ccatcccaac caacttcacc atctctgtga ccacagagat tctgcctgtg agtatgacca 6180 agacctctgt ggactgtaca atgtatatct gtggagacag cacagagtgt agcaacctgc 6240 tgctccaata tggctccttc tgtacccaac ttaacagggc tctgacaggc attgctgtgg 6300 aacaggacaa gaacacccag gaggtgtttg cccaggtgaa gcagatttac aagacacctc 6360 caatcaagga ctttggaggc ttcaacttca gccagattct gcctgaccca agcaagccaa 6420 gcaagaggtc cttcattgag gacctgctgt tcaacaaggt gaccctggct gatgctggct 6480 tcatcaagca atatggagac tgtctgggag acattgctgc cagggacctg atttgtgccc 6540 agaagttcaa tggactgaca gtgctgcctc cactgctgac agatgagatg attgcccaat 6600 acacctctgc cctgctggct ggcaccatca cctctggctg gacctttgga gcaggagcag 6660 ccctccaaat cccatttgct atgcagatgg cttacaggtt caatggcatt ggagtgaccc 6720 agaatgtgct ctatgagaac cagaaactga ttgccaacca gttcaactct gccattggca 6780 agattcagga ctccctgtcc agcacagcct ctgccctggg caaactccaa gatgtggtga 6840 accagaatgc ccaggctctg aacaccctgg tgaagcaact ttccagcaac tttggagcca 6900 tctcctctgt gctgaatgac atcctgagca gactggacaa ggtggaggct gaggtccaga 6960 ttgacagact gattacaggc agactccaat ccctccaaac ctatgtgacc caacaactta 7020 tcagggctgc tgagattagg gcatctgcca acctggctgc caccaagatg agtgagtgtg 7080 tgctgggaca aagcaagagg gtggacttct gtggcaaggg ctaccacctg atgagttttc 7140 cacagtctgc ccctcatgga gtggtgttcc tgcatgtgac ctatgtgcct gcccaggaga 7200 agaacttcac cacagcccct gccatctgcc atgatggcaa ggctcacttt ccaagggagg 7260 gagtgtttgt gagcaatggc acccactggt ttgtgaccca gaggaacttc tatgaaccac 7320 agattatcac cacagacaac acctttgtgt ctggcaactg tgatgtggtg attggcattg 7380 tgaacaacac agtctatgac ccactccaac ctgaactgga ctccttcaag gaggaactgg 7440 acaaatactt caagaaccac accagccctg atgtggacct gggagacatc tctggcatca 7500 atgcctctgt ggtgaacatc cagaaggaga ttgacagact gaatgaggtg gctaagaacc 7560 tgaatgagtc cctgattgac ctccaagaac tgggcaaata tgaacaatac atcaagtggc 7620 catgaaaatt gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 7680 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 7740 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7800 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac 7860 gcggatctgg gcgtggttaa gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt 7920 ttgtatctgt tttgcagcag ccgccgccgc catgagcacc aactcgtttg atggaagcat 7980 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 8040 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 8100 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 8160 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 8220 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 8280 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 8340 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 8400 ttggatttgg atcaagcaag tgtcttgctg tctttattta ggggttttgc gcgcgcggta 8460 ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt attttttcca ggacgtggta 8520 aaggtgactc tggatgttca gatacatggg cataagcccg tctctggggt ggaggtagca 8580 ccactgcaga gcttcatgct gcggggtggt gttgtagatg atccagtcgt agcaggagcg 8640 ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg attgccaggg gcaggccctt 8700 ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc atacgtgggg atatgagatg 8760 catcttggac tgtattttta ggttggctat gttcccagcc atatccctcc ggggattcat 8820 gttgtgcaga accaccagca cagtgtatcc ggtgcacttg ggaaatttgt catgtagctt 8880 agaaggaaat gcgtggaaga acttggagac gcccttgtga cctccaagat tttccatgca 8940 ttcgtccata atgatggcaa tgggcccacg ggcggcggcc tgggcgaaga tatttctggg 9000 atcactaacg tcatagttgt gttccaggat gagatcgtca taggccattt ttacaaagcg 9060 cgggcggagg gtgccagact gcggtataat ggttccatcc ggcccagggg cgtagttacc 9120 ctcacagatt tgcatttccc acgctttgag ttcagatggg gggatcatgt ctacctgcgg 9180 ggcgatgaag aaaacggttt ccggggtagg ggagatcagc tgggaagaaa gcaggttcct 9240 gagcagctgc gacttaccgc agccggtggg cccgtaaatc acacctatta ccggctgcaa 9300 ctggtagtta agagagctgc agctgccgtc atccctgagc aggggggcca cttcgttaag 9360 catgtccctg actcgcatgt tttccctgac caaatccgcc agaaggcgct cgccgcccag 9420 cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt ttgagaccgt ccgccgtagg 9480 catgcttttg agcgtttgac caagcagttc caggcggtcc cacagctcgg tcacctgctc 9540 tacggcatct cgatccagca tatctcctcg tttcgcgggt tggggcggct ttcgctgtac 9600 ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt ctttccacgg gcgcagggtc 9660 ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc cgggctgcgc gctggccagg 9720 gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc ggtcttcgcc ctgcgcgtcg 9780 gccaggtagc atttgaccat ggtgtcatag tccagcccct ccgcggcgtg gcccttggcg 9840 cgcagcttgc ccttggagga ggcgccgcac gaggggcagt gcagactttt gagggcgtag 9900 agcttgggcg cgagaaatac cgattccggg gagtaggcat ccgcgccgca ggccccgcag 9960 acggtctcgc attccacgag ccaggtgagc tctggccgtt cggggtcaaa aaccaggttt 10020 cccccatgct ttttgatgcg tttcttacct ctggtttcca tgagccggtg tccacgctcg 10080 gtgacgaaaa ggctgtccgt gtccccgtat acagacttga gaggcctgtc ctcgagcggt 10140 gttccgcggt cctcctcgta tagaaactcg gaccactctg agacaaaggc tcgcgtccag 10200 gccagcacga aggaggctaa gtgggagggg tagcggtcgt tgtccactag ggggtccact 10260 cgctccaggg tgtgaagaca catgtcgccc tcttcggcat caaggaaggt gattggtttg 10320 taggtgtagg ccacgtgacc gggtgttcct gaaggggggc tataaaaggg ggtgggggcg 10380 cgttcgtcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac 10440 tccctctgaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag 10500 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcatc catctggtca 10560 gaaaagacaa tctttttgtt gtcaagcttg gtggcaaacg acccgtagag ggcgttggac 10620 agcaacttgg cgatggagcg cagggtttgg tttttgtcgc gatcggcgcg ctccttggcc 10680 gcgatgttta gctgcacgta ttcgcgcgca acgcaccgcc attcgggaaa gacggtggtg 10740 cgctcgtcgg gcaccaggtg cacgcgccaa ccgcggttgt gcagggtgac aaggtcaacg 10800 ctggtggcta cctctccgcg taggcgctcg ttggtccagc agaggcggcc gcccttgcgc 10860 gagcagaatg gcggtagggg gtctagctgc gtctcgtccg gggggtctgc gtccacggta 10920 aagaccccgg gcagcaggcg cgcgtcgaag tagtctatct tgcatccttg caagtctagc 10980 gcctgctgcc atgcgcgggc ggcaagcgcg cgctcgtatg ggttgagtgg gggaccccat 11040 ggcatggggt gggtgagcgc ggaggcgtac atgccgcaaa tgtcgtaaac gtagaggggc 11100 tctctgagta ttccaagata tgtagggtag catcttccac cgcggatgct ggcgcgcacg 11160 taatcgtata gttcgtgcga gggagcgagg aggtcgggac cgaggttgct acgggcgggc 11220 tgctctgctc ggaagactat ctgcctgaag atggcatgtg agttggatga tatggttgga 11280 cgctggaaga cgttgaagct ggcgtctgtg agacctaccg cgtcacgcac gaaggaggcg 11340 taggagtcgc gcagcttgtt gaccagctcg gcggtgacct gcacgtctag ggcgcagtag 11400 tccagggttt ccttgatgat gtcatactta tcctgtccct tttttttcca cagctcgcgg 11460 ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc gtcggcctcc 11520 gaacggtaag agcctagcat gtagaactgg ttgacggcct ggtaggcgca gcatcccttt 11580 tctacgggta gcgcgtatgc ctgcgcggcc ttccggagcg aggtgtgggt gagcgcaaag 11640 gtgtccctga ccatgacttt gaggtactgg tatttgaagt cagtgtcgtc gcatccgccc 11700 tgctcccaga gcaaaaagtc cgtgcgcttt ttggaacgcg gatttggcag ggcgaaggtg 11760 acatcgttga agagtatctt tcccgcgcga ggcataaagt tgcgtgtgat gcggaagggt 11820 cccggcacct cggaacggtt gttaattacc tgggcggcga gcacgatctc gtcaaagccg 11880 ttgatgttgt ggcccacaat gtaaagttcc aagaagcgcg ggatgccctt gatggaaggc 11940 aattttttaa gttcctcgta ggtgagctct tcaggggagc tgagcccgtg ctctgaaagg 12000 gcccagtctg caagatgagg gttggaagcg acgaatgagc tccacaggtc acgggccatt 12060 agcatttgca ggtggtcgcg aaaggtccta aactggcgac ctatggccat tttttctggg 12120 gtgatgcagt agaaggtaag cgggtcttgt tcccagcggt cccatccaag gttcgcggct 12180 aggtctcgcg cggcagtcac tagaggctca tctccgccga acttcatgac cagcatgaag 12240 ggcacgagct gcttcccaaa ggcccccatc caagtatagg tctctacatc gtaggtgaca 12300 aagagacgct cggtgcgagg atgcgagccg atcgggaaga actggatctc ccgccaccaa 12360 ttggaggagt ggctattgat gtggtgaaag tagaagtccc tgcgacgggc cgaacactcg 12420 tgctggcttt tgtaaaaacg tgcgcagtac tggcagcggt gcacgggctg tacatcctgc 12480 acgaggttga cctgacgacc gcgcacaagg aagcagagtg ggaatttgag cccctcgcct 12540 ggcgggtttg gctggtggtc ttctacttcg gctgcttgtc cttgaccgtc tggctgctcg 12600 aggggagtta cggtggatcg gaccaccacg ccgcgcgagc ccaaagtcca gatgtccgcg 12660 cgcggcggtc ggagcttgat gacaacatcg cgcagatggg agctgtccat ggtctggagc 12720 tcccgcggcg tcaggtcagg cgggagctcc tgcaggttta cctcgcatag acgggtcagg 12780 gcgcgggcta gatccaggtg atacctaatt tccaggggct ggttggtggc ggcgtcgatg 12840 gcttgcaaga ggccgcatcc ccgcggcgcg actacggtac cgcgcggcgg gcggtgggcc 12900 gcgggggtgt ccttggatga tgcatctaaa agcggtgacg cgggcgagcc cccggaggta 12960 gggggggctc cggacccgcc gggagagggg gcaggggcac gtcggcgccg cgcgcgggca 13020 ggagctggtg ctgcgcgcgt aggttgctgg cgaacgcgac gacgcggcgg ttgatctcct 13080 gaatctggcg cctctgcgtg aagacgacgg gcccggtgag cttgaacctg aaagagagtt 13140 cgacagaatc aatttcggtg tcgttgacgg cggcctggcg caaaatctcc tgcacgtctc 13200 ctgagttgtc ttgataggcg atctcggcca tgaactgctc gatctcttcc tcctggagat 13260 ctccgcgtcc ggctcgctcc acggtggcgg cgaggtcgtt ggaaatgcgg gccatgagct 13320 gcgagaaggc gttgaggcct ccctcgttcc agacgcggct gtagaccacg cccccttcgg 13380 catcgcgggc gcgcatgacc acctgcgcga gattgagctc cacgtgccgg gcgaagacgg 13440 cgtagtttcg caggcgctga aagaggtagt tgagggtggt ggcggtgtgt tctgccacga 13500 agaagtacat aacccagcgt cgcaacgtgg attcgttgat atcccccaag gcctcaaggc 13560 gctccatggc ctcgtagaag tccacggcga agttgaaaaa ctgggagttg cgcgccgaca 13620 cggttaactc ctcctccaga agacggatga gctcggcgac agtgtcgcgc acctcgcgct 13680 caaaggctac aggggcctct tcttcttctt caatctcctc ttccataagg gcctcccctt 13740 cttcttcttc tggcggcggt gggggagggg ggacacggcg gcgacgacgg cgcaccggga 13800 ggcggtcgac aaagcgctcg atcatctccc cgcggcgacg gcgcatggtc tcggtgacgg 13860 cgcggccgtt ctcgcggggg cgcagttgga agacgccgcc cgtcatgtcc cggttatggg 13920 ttggcggggg gctgccatgc ggcagggata cggcgctaac gatgcatctc aacaattgtt 13980 gtgtaggtac tccgccgccg agggacctga gcgagtccgc atcgaccgga tcggaaaacc 14040 tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc gtggcgggcg 14100 gcagcgggcg gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg taattaaagt 14160 aggcggtctt gagacggcgg atggtcgaca gaagcaccat gtccttgggt ccggcctgct 14220 gaatgcgcag gcggtcggcc atgccccagg cttcgttttg acatcggcgc aggtctttgt 14280 agtagtcttg catgagcctt tctaccggca cttcttcttc tccttcctct tgtcctgcat 14340 ctcttgcatc tatcgctgcg gcggcggcgg agtttggccg taggtggcgc cctcttcctc 14400 ccatgcgtgt gaccccgaag cccctcatcg gctgaagcag ggctaggtcg gcgacaacgc 14460 gctcggctaa tatggcctgc tgcacctgcg tgagggtaga ctggaagtca tccatgtcca 14520 caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca gttggccata acggaccagt 14580 taacggtctg gtgacccggc tgcgagagct cggtgtacct gagacgcgag taagccctcg 14640 agtcaaatac gtagtcgttg caagtccgca ccaggtactg gtatcccacc aaaaagtgcg 14700 gcggcggctg gcggtagagg ggccagcgta gggtggccgg ggctccgggg gcgagatctt 14760 ccaacataag gcgatgatat ccgtagatgt acctggacat ccaggtgatg ccggcggcgg 14820 tggtggaggc gcgcggaaag tcgcggacgc ggttccagat gttgcgcagc ggcaaaaagt 14880 gctccatggt cgggacgctc tggccggtca ggcgcgcgca atcgttgacg ctctagcgtg 14940 caaaaggaga gcctgtaagc gggcactctt ccgtggtctg gtggataaat tcgcaagggt 15000 atcatggcgg acgaccgggg ttcgagcccc gtatccggcc gtccgccgtg atccatgcgg 15060 ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac aacgggggag tgctcctttt 15120 ggcttccttc caggcgcggc ggctgctgcg ctagcttttt tggccactgg ccgcgcgcag 15180 cgtaagcggt taggctggaa agcgaaagca ttaagtggct cgctccctgt agccggaggg 15240 ttattttcca agggttgagt cgcgggaccc ccggttcgag tctcggaccg gccggactgc 15300 ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc ttgcaaattc ctccggaaac 15360 agggacgagc cccttttttg cttttcccag atgcatccgg tgctgcggca gatgcgcccc 15420 cctcctcagc agcggcaaga gcaagagcag cggcagacat gcagggcacc ctcccctcct 15480 cctaccgcgt caggaggggc gacatccgcg gttgacgcgg cagcagatgg tgattacgaa 15540 cccccgcggc gccgggcccg gcactacctg gacttggagg agggcgaggg cctggcgcgg 15600 ctaggagcgc cctctcctga gcggcaccca agggtgcagc tgaagcgtga tacgcgtgag 15660 gcgtacgtgc cgcggcagaa cctgtttcgc gaccgcgagg gagaggagcc cgaggagatg 15720 cgggatcgaa agttccacgc agggcgcgag ctgcggcatg gcctgaatcg cgagcggttg 15780 ctgcgcgagg aggactttga gcccgacgcg cgaaccggga ttagtcccgc gcgcgcacac 15840 gtggcggccg ccgacctggt aaccgcatac gagcagacgg tgaaccagga gattaacttt 15900 caaaaaagct ttaacaacca cgtgcgtacg cttgtggcgc gcgaggaggt ggctatagga 15960 ctgatgcatc tgtgggactt tgtaagcgcg ctggagcaaa acccaaatag caagccgctc 16020 atggcgcagc tgttccttat agtgcagcac agcagggaca acgaggcatt cagggatgcg 16080 ctgctaaaca tagtagagcc cgagggccgc tggctgctcg atttgataaa catcctgcag 16140 agcatagtgg tgcaggagcg cagcttgagc ctggctgaca aggtggccgc catcaactat 16200 tccatgctta gcctgggcaa gttttacgcc cgcaagatat accatacccc ttacgttccc 16260 atagacaagg aggtaaagat cgaggggttc tacatgcgca tggcgctgaa ggtgcttacc 16320 ttgagcgacg acctgggcgt ttatcgcaac gagcgcatcc acaaggccgt gagcgtgagc 16380 cggcggcgcg agctcagcga ccgcgagctg atgcacagcc tgcaaagggc cctggctggc 16440 acgggcagcg gcgatagaga ggccgagtcc tactttgacg cgggcgctga cctgcgctgg 16500 gccccaagcc gacgcgccct ggaggcagct ggggccggac ctgggctggc ggtggcaccc 16560 gcgcgcgctg gcaacgtcgg cggcgtggag gaatatgacg aggacgatga gtacgagcca 16620 gaggacggcg agtactaagc ggtgatgttt ctgatcagat gatgcaagac gcaacggacc 16680 cggcggtgcg ggcggcgctg cagagccagc cgtccggcct taactccacg gacgactggc 16740 gccaggtcat ggaccgcatc atgtcgctga ctgcgcgcaa tcctgacgcg ttccggcagc 16800 agccgcaggc caaccggctc tccgcaattc tggaagcggt ggtcccggcg cgcgcaaacc 16860 ccacgcacga gaaggtgctg gcgatcgtaa acgcgctggc cgaaaacagg gccatccggc 16920 ccgacgaggc cggcctggtc tacgacgcgc tgcttcagcg cgtggctcgt tacaacagcg 16980 gcaacgtgca gaccaacctg gaccggctgg tgggggatgt gcgcgaggcc gtggcgcagc 17040 gtgagcgcgc gcagcagcag ggcaacctgg gctccatggt tgcactaaac gccttcctga 17100 gtacacagcc cgccaacgtg ccgcggggac aggaggacta caccaacttt gtgagcgcac 17160 tgcggctaat ggtgactgag acaccgcaaa gtgaggtgta ccagtctggg ccagactatt 17220 ttttccagac cagtagacaa ggcctgcaga ccgtaaacct gagccaggct ttcaaaaact 17280 tgcaggggct gtggggggtg cgggctccca caggcgaccg cgcgaccgtg tctagcttgc 17340 tgacgcccaa ctcgcgcctg ttgctgctgc taatagcgcc cttcacggac agtggcagcg 17400 tgtcccggga cacataccta ggtcacttgc tgacactgta ccgcgaggcc ataggtcagg 17460 cgcatgtgga cgagcatact ttccaggaga ttacaagtgt cagccgcgcg ctggggcagg 17520 aggacacggg cagcctggag gcaaccctaa actacctgct gaccaaccgg cggcagaaga 17580 tcccctcgtt gcacagttta aacagcgagg aggagcgcat tttgcgctac gtgcagcaga 17640 gcgtgagcct taacctgatg cgcgacgggg taacgcccag cgtggcgctg gacatgaccg 17700 cgcgcaacat ggaaccgggc atgtatgcct caaaccggcc gtttatcaac cgcctaatgg 17760 actacttgca tcgcgcggcc gccgtgaacc ccgagtattt caccaatgcc atcttgaacc 17820 cgcactggct accgccccct ggtttctaca ccgggggatt cgaggtgccc gagggtaacg 17880 atggattcct ctgggacgac atagacgaca gcgtgttttc cccgcaaccg cagaccctgc 17940 tagagttgca acagcgcgag caggcagagg cggcgctgcg aaaggaaagc ttccgcaggc 18000 caagcagctt gtccgatcta ggcgctgcgg ccccgcggtc agatgctagt agcccatttc 18060 caagcttgat agggtctctt accagcactc gcaccacccg cccgcgcctg ctgggcgagg 18120 aggagtacct aaacaactcg ctgctgcagc cgcagcgcga aaaaaacctg cctccggcat 18180 ttcccaacaa cgggatagag agcctagtgg acaagatgag tagatggaag acgtacgcgc 18240 aggagcacag ggacgtgcca ggcccgcgcc cgcccacccg tcgtcaaagg cacgaccgtc 18300 agcggggtct ggtgtgggag gacgatgact cggcagacga cagcagcgtc ctggatttgg 18360 gagggagtgg caacccgttt gcgcaccttc gccccaggct ggggagaatg ttttaaaaaa 18420 aaaaaagcat gatgcaaaat aaaaaactca ccaaggccat ggcaccgagc gttggttttc 18480 ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag gaaggtcctc ctccctccta 18540 cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg ggttctccct tcgatgctcc 18600 cctggacccg ccgtttgtgc ctccgcggta cctgcggcct accgggggga gaaacagcat 18660 ccgttactct gagttggcac ccctattcga caccacccgt gtgtacctgg tggacaacaa 18720 gtcaacggat gtggcatccc tgaactacca gaacgaccac agcaactttc tgaccacggt 18780 cattcaaaac aatgactaca gcccggggga ggcaagcaca cagaccatca atcttgacga 18840 ccggtcgcac tggggcggcg acctgaaaac catcctgcat accaacatgc caaatgtgaa 18900 cgagttcatg tttaccaata agtttaaggc gcgggtgatg gtgtcgcgct tgcctactaa 18960 ggacaatcag gtggagctga aatacgagtg ggtggagttc acgctgcccg agggcaacta 19020 ctccgagacc atgaccatag accttatgaa caacgcgatc gtggagcact acttgaaagt 19080 gggcagacag aacggggttc tggaaagcga catcggggta aagtttgaca cccgcaactt 19140 cagactgggg tttgaccccg tcactggtct tgtcatgcct ggggtatata caaacgaagc 19200 cttccatcca gacatcattt tgctgccagg atgcggggtg gacttcaccc acagccgcct 19260 gagcaacttg ttgggcatcc gcaagcggca acccttccag gagggcttta ggatcaccta 19320 cgatgatctg gagggtggta acattcccgc actgttggat gtggacgcct accaggcgag 19380 cttgaaagat gacaccgaac agggcggggg tggcgcaggc ggcagcaaca gcagtggcag 19440 cggcgcggaa gagaactcca acgcggcagc cgcggcaatg cagccggtgg aggacatgaa 19500 cgatcatgcc attcgcggcg acacctttgc cacacgggct gaggagaagc gcgctgaggc 19560 cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc gaggtcgaga agcctcagaa 19620 gaaaccggtg atcaaacccc tgacagagga cagcaagaaa cgcagttaca acctaataag 19680 caatgacagc accttcaccc agtaccgcag ctggtacctt gcatacaact acggcgaccc 19740 tcagaccgga atccgctcat ggaccctgct ttgcactcct gacgtaacct gcggctcgga 19800 gcaggtctac tggtcgttgc cagacatgat gcaagacccc gtgaccttcc gctccacgcg 19860 ccagatcagc aactttccgg tggtgggcgc cgagctgttg cccgtgcact ccaagagctt 19920 ctacaacgac caggccgtct actcccaact catccgccag tttacctctc tgacccacgt 19980 gttcaatcgc tttcccgaga accagatttt ggcgcgcccg ccagccccca ccatcaccac 20040 cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg ctaccgctgc gcaacagcat 20100 cggaggagtc cagcgagtga ccattactga cgccagacgc cgcacctgcc cctacgttta 20160 caaggccctg ggcatagtct cgccgcgcgt cctatcgagc cgcacttttt gagcaagcat 20220 gtccatcctt atatcgccca gcaataacac aggctggggc ctgcgcttcc caagcaagat 20280 gtttggcggg gccaagaagc gctccgacca acacccagtg cgcgtgcgcg ggcactaccg 20340 cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc accaccgtcg atgacgccat 20400 cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg ccgccaccag tgtccacagt 20460 ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc tatgctaaaa tgaagagacg 20520 gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc actgccgccc aacgcgcggc 20580 ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg gcggccatgc gggccgctcg 20640 aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc aggcgacgag cggccgccgc 20700 agcagccgcg gccattagtg ctatgactca gggtcgcagg ggcaacgtgt attgggtgcg 20760 cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc cccccgcgca actagattgc 20820 aagaaaaaac tacttagact cgtactgttg tatgtatcca gcggcggcgg cgcgcaacga 20880 agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag gtcatcgcgc cggagatcta 20940 tggccccccg aagaaggaag agcaggatta caagccccga aagctaaagc gggtcaaaaa 21000 gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg gaactgctgc acgctaccgc 21060 gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa cgtgttttgc gacccggcac 21120 caccgtagtc tttacgcccg gtgagcgctc cacccgcacc tacaagcgcg tgtatgatga 21180 ggtgtacggc gacgaggacc tgcttgagca ggccaacgag cgcctcgggg agtttgccta 21240 cggaaagcgg cataaggaca tgctggcgtt gccgctggac gagggcaacc caacacctag 21300 cctaaagccc gtaacactgc agcaggtgct gcccgcgctt gcaccgtccg aagaaaagcg 21360 cggcctaaag cgcgagtctg gtgacttggc acccaccgtg cagctgatgg tacccaagcg 21420 ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa cctgggctgg agcccgaggt 21480 ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc gtgcagaccg tggacgttca 21540 gatacccact accagtagca ccagtattgc caccgccaca gagggcatgg agacacaaac 21600 gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag gcggtcgctg cggccgcgtc 21660 caagacctct acggaggtgc aaacggaccc gtggatgttt cgcgtttcag ccccccggcg 21720 cccgcgccgt tcgaggaagt acggcgccgc cagcgcgcta ctgcccgaat atgccctaca 21780 tccttccatt gcgcctaccc ccggctatcg tggctacacc taccgcccca gaagacgagc 21840 aactacccga cgccgaacca ccactggaac ccgccgccgc cgtcgccgtc gccagcccgt 21900 gctggccccg atttccgtgc gcagggtggc tcgcgaagga ggcaggaccc tggtgctgcc 21960 aacagcgcgc taccacccca gcatcgttta aaagccggtc tttgtggttc ttgcagatat 22020 ggccctcacc tgccgcctcc gtttcccggt gccgggattc cgaggaagaa tgcaccgtag 22080 gaggggcatg gccggccacg gcctgacggg cggcatgcgt cgtgcgcacc accggcggcg 22140 gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc ctccttattc cactgatcgc 22200 cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc ttgcaggcgc agagacactg 22260 attaaaaaca agttgcatgt ggaaaaatca aaataaaaag tctggactct cacgctcgct 22320 tggtcctgta actattttgt agaatggaag acatcaactt tgcgtctctg gccccgcgac 22380 acggctcgcg cccgttcatg ggaaactggc aagatatcgg caccagcaat atgagcggtg 22440 gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa tttcggttcc accgttaaga 22500 actatggcag caaggcctgg aacagcagca caggccagat gctgagggat aagttgaaag 22560 agcaaaattt ccaacaaaag gtggtagatg gcctggcctc tggcattagc ggggtggtgg 22620 acctggccaa ccaggcagtg caaaataaga ttaacagtaa gcttgatccc cgccctcccg 22680 tagaggagcc tccaccggcc gtggagacag tgtctccaga ggggcgtggc gaaaagcgtc 22740 cgcgccccga cagggaagaa actctggtga cgcaaataga cgagcctccc tcgtacgagg 22800 aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc gcccatggct accggagtgc 22860 tgggccagca cacacccgta acgctggacc tgcctccccc cgccgacacc cagcagaaac 22920 ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc tagccgcgcg tccctgcgcc 22980 gcgccgccag cggtccgcga tcgttgcggc ccgtagccag tggcaactgg caaagcacac 23040 tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg ccgacgatgc ttctgatagc 23100 taacgtgtcg tatgtgtgtc atgtatgcgt ccatgtcgcc gccagaggag ctgctgagcc 23160 gccgcgcgcc cgctttccaa gatggctacc ccttcgatga tgccgcagtg gtcttacatg 23220 cacatctcgg gccaggacgc ctcggagtac ctgagccccg ggctggtgca gtttgcccgc 23280 gccaccgaga cgtacttcag cctgaataac aagtttagaa accccacggt ggcgcctacg 23340 cacgacgtga ccacagaccg gtcccagcgt ttgacgctgc ggttcatccc tgtggaccgt 23400 gaggatactg cgtactcgta caaggcgcgg ttcaccctag ctgtgggtga taaccgtgtg 23460 ctggacatgg cttccacgta ctttgacatc cgcggcgtgc tggacagggg ccctactttt 23520 aagccctact ctggcactgc ctacaacgcc ctggctccca agggtgcccc aaatccttgc 23580 gaatgggatg aagctgctac tgctcttgaa ataaacctag aagaagagga cgatgacaac 23640 gaagacgaag tagacgagca agctgagcag caaaaaactc acgtatttgg gcaggcgcct 23700 tattctggta taaatattac aaaggagggt attcaaatag gtgtcgaagg tcaaacacct 23760 aaatatgccg ataaaacatt tcaacctgaa cctcaaatag gagaatctca gtggtacgaa 23820 acagaaatta atcatgcagc tgggagagtc ctaaaaaaga ctaccccaat gaaaccatgt 23880 tacggttcat atgcaaaacc cacaaatgaa aatggagggc aaggcattct tgtaaagcaa 23940 caaaatggaa agctagaaag tcaagtggaa atgcaatttt tctcaactac tgaggcagcc 24000 gcaggcaatg gtgataactt gactcctaaa gtggtattgt acagtgaaga tgtagatata 24060 gaaaccccag acactcatat ttcttacatg cccactatta aggaaggtaa ctcacgagaa 24120 ctaatgggcc aacaatctat gcccaacagg cctaattaca ttgcttttag ggacaatttt 24180 attggtctaa tgtattacaa cagcacgggt aatatgggtg ttctggcggg ccaagcatcg 24240 cagttgaatg ctgttgtaga tttgcaagac agaaacacag agctttcata ccagcttttg 24300 cttgattcca ttggtgatag aaccaggtac ttttctatgt ggaatcaggc tgttgacagc 24360 tatgatccag atgttagaat tattgaaaat catggaactg aagatgaact tccaaattac 24420 tgctttccac tgggaggtgt gattaataca gagactctta ccaaggtaaa acctaaaaca 24480 ggtcaggaaa atggatggga aaaagatgct acagaatttt cagataaaaa tgaaataaga 24540 gttggaaata attttgccat ggaaatcaat ctaaatgcca acctgtggag aaatttcctg 24600 tactccaaca tagcgctgta tttgcccgac aagctaaagt acagtccttc caacgtaaaa 24660 atttctgata acccaaacac ctacgactac atgaacaagc gagtggtggc tcccgggcta 24720 gtggactgct acattaacct tggagcacgc tggtcccttg actatatgga caacgtcaac 24780 ccatttaacc accaccgcaa tgctggcctg cgctaccgct caatgttgct gggcaatggt 24840 cgctatgtgc ccttccacat ccaggtgcct cagaagttct ttgccattaa aaacctcctt 24900 ctcctgccgg gctcatacac ctacgagtgg aacttcagga aggatgttaa catggttctg 24960 cagagctccc taggaaatga cctaagggtt gacggagcca gcattaagtt tgatagcatt 25020 tgcctttacg ccaccttctt ccccatggcc cacaacaccg cctccacgct tgaggccatg 25080 cttagaaacg acaccaacga ccagtccttt aacgactatc tctccgccgc caacatgctc 25140 taccctatac ccgccaacgc taccaacgtg cccatatcca tcccctcccg caactgggcg 25200 gctttccgcg gctgggcctt cacgcgcctt aagactaagg aaaccccatc actgggctcg 25260 ggctacgacc cttattacac ctactctggc tctataccct acctagatgg aaccttttac 25320 ctcaaccaca cctttaagaa ggtggccatt acctttgact cttctgtcag ctggcctggc 25380 aatgaccgcc tgcttacccc caacgagttt gaaattaagc gctcagttga cggggagggt 25440 tacaacgttg cccagtgtaa catgaccaaa gactggttcc tggtacaaat gctagctaac 25500 tataacattg gctaccaggg cttctatatc ccagagagct acaaggaccg catgtactcc 25560 ttctttagaa acttccagcc catgagccgt caggtggtgg atgatactaa atacaaggac 25620 taccaacagg tgggcatcct acaccaacac aacaactctg gatttgttgg ctaccttgcc 25680 cccaccatgc gcgaaggaca ggcctaccct gctaacttcc cctatccgct tataggcaag 25740 accgcagttg acagcattac ccagaaaaag tttctttgcg atcgcaccct ttggcgcatc 25800 ccattctcca gtaactttat gtccatgggc gcactcacag acctgggcca aaaccttctc 25860 tacgccaact ccgcccacgc gctagacatg acttttgagg tggatcccat ggacgagccc 25920 acccttcttt atgttttgtt tgaagtcttt gacgtggtcc gtgtgcacca gccgcaccgc 25980 ggcgtcatcg aaaccgtgta cctgcgcacg cccttctcgg ccggcaacgc cacaacataa 26040 agaagcaagc aacatcaaca acagctgccg ccatgggctc cagtgagcag gaactgaaag 26100 ccattgtcaa agatcttggt tgtgggccat attttttggg cacctatgac aagcgctttc 26160 caggctttgt ttctccacac aagctcgcct gcgccatagt caatacggcc ggtcgcgaga 26220 ctgggggcgt acactggatg gcctttgcct ggaacccgca ctcaaaaaca tgctacctct 26280 ttgagccctt tggcttttct gaccagcgac tcaagcaggt ttaccagttt gagtacgagt 26340 cactcctgcg ccgtagcgcc attgcttctt cccccgaccg ctgtataacg ctggaaaagt 26400 ccacccaaag cgtacagggg cccaactcgg ccgcctgtgg actattctgc tgcatgtttc 26460 tccacgcctt tgccaactgg ccccaaactc ccatggatca caaccccacc atgaacctta 26520 ttaccggggt acccaactcc atgctcaaca gtccccaggt acagcccacc ctgcgtcgca 26580 accaggaaca gctctacagc ttcctggagc gccactcgcc ctacttccgc agccacagtg 26640 cgcagattag gagcgccact tctttttgtc acttgaaaaa catgtaaaaa taatgtacta 26700 gagacacttt caataaaggc aaatgctttt atttgtacac tctcgggtga ttatttaccc 26760 ccacccttgc cgtctgcgcc gtttaaaaat caaaggggtt ctgccgcgca tcgctatgcg 26820 ccactggcag ggacacgttg cgatactggt gtttagtgct ccacttaaac tcaggcacaa 26880 ccatccgcgg cagctcggtg aagttttcac tccacaggct gcgcaccatc accaacgcgt 26940 ttagcaggtc gggcgccgat atcttgaagt cgcagttggg gcctccgccc tgcgcgcgcg 27000 agttgcgata cacagggttg cagcactgga acactatcag cgccgggtgg tgcacgctgg 27060 ccagcacgct cttgtcggag atcagatccg cgtccaggtc ctccgcgttg ctcagggcga 27120 acggagtcaa ctttggtagc tgccttccca aaaagggcgc gtgcccaggc tttgagttgc 27180 actcgcaccg tagtggcatc aaaaggtgac cgtgcccggt ctgggcgtta ggatacagcg 27240 cctgcataaa agccttgatc tgcttaaaag ccacctgagc ctttgcgcct tcagagaaga 27300 acatgccgca agacttgccg gaaaactgat tggccggaca ggccgcgtcg tgcacgcagc 27360 accttgcgtc ggtgttggag atctgcacca catttcggcc ccaccggttc ttcacgatct 27420 tggccttgct agactgctcc ttcagcgcgc gctgcccgtt ttcgctcgtc acatccattt 27480 caatcacgtg ctccttattt atcataatgc ttccgtgtag acacttaagc tcgccttcga 27540 tctcagcgca gcggtgcagc cacaacgcgc agcccgtggg ctcgtgatgc ttgtaggtca 27600 cctctgcaaa cgactgcagg tacgcctgca ggaatcgccc catcatcgtc acaaaggtct 27660 tgttgctggt gaaggtcagc tgcaacccgc ggtgctcctc gttcagccag gtcttgcata 27720 cggccgccag agcttccact tggtcaggca gtagtttgaa gttcgccttt agatcgttat 27780 ccacgtggta cttgtccatc agcgcgcgcg cagcctccat gcccttctcc cacgcagaca 27840 cgatcggcac actcagcggg ttcatcaccg taatttcact ttccgcttcg ctgggctctt 27900 cctcttcctc ttgcgtccgc ataccacgcg ccactgggtc gtcttcattc agccgccgca 27960 ctgtgcgctt acctcctttg ccatgcttga ttagcaccgg tgggttgctg aaacccacca 28020 tttgtagcgc cacatcttct ctttcttcct cgctgtccac gattacctct ggtgatggcg 28080 ggcgctcggg cttgggagaa gggcgcttct ttttcttctt gggcgcaatg gccaaatccg 28140 ccgccgaggt cgatggccgc gggctgggtg tgcgcggcac cagcgcgtct tgtgatgagt 28200 cttcctcgtc ctcggactcg atacgccgcc tcatccgctt ttttgggggc gcccggggag 28260 gcggcggcga cggggacggg gacgacacgt cctccatggt tgggggacgt cgcgccgcac 28320 cgcgtccgcg ctcgggggtg gtttcgcgct gctcctcttc ccgactggcc atttccttct 28380 cctataggca gaaaaagatc atggagtcag tcgagaagaa ggacagccta accgccccct 28440 ctgagttcgc caccaccgcc tccaccgatg ccgccaacgc gcctaccacc ttccccgtcg 28500 aggcaccccc gcttgaggag gaggaagtga ttatcgagca ggacccaggt tttgtaagcg 28560 aagacgacga ggaccgctca gtaccaacag aggataaaaa gcaagaccag gacaacgcag 28620 aggcaaacga ggaacaagtc gggcgggggg acgaaaggca tggcgactac ctagatgtgg 28680 gagacgacgt gctgttgaag catctgcagc gccagtgcgc cattatctgc gacgcgttgc 28740 aagagcgcag cgatgtgccc ctcgccatag cggatgtcag ccttgcctac gaacgccacc 28800 tattctcacc gcgcgtaccc cccaaacgcc aagaaaacgg cacatgcgag cccaacccgc 28860 gcctcaactt ctaccccgta tttgccgtgc cagaggtgct tgccacctat cacatctttt 28920 tccaaaactg caagataccc ctatcctgcc gtgccaaccg cagccgagcg gacaagcagc 28980 tggccttgcg gcagggcgct gtcatacctg atatcgcctc gctcaacgaa gtgccaaaaa 29040 tctttgaggg tcttggacgc gacgagaagc gcgcggcaaa cgctctgcaa caggaaaaca 29100 gcgaaaatga aagtcactct ggagtgttgg tggaactcga gggtgacaac gcgcgcctag 29160 ccgtactaaa acgcagcatc gaggtcaccc actttgccta cccggcactt aacctacccc 29220 ccaaggtcat gagcacagtc atgagtgagc tgatcgtgcg ccgtgcgcag cccctggaga 29280 gggatgcaaa tttgcaagaa caaacagagg agggcctacc cgcagttggc gacgagcagc 29340 tagcgcgctg gcttcaaacg cgcgagcctg ccgacttgga ggagcgacgc aaactaatga 29400 tggccgcagt gctcgttacc gtggagcttg agtgcatgca gcggttcttt gctgacccgg 29460 agatgcagcg caagctagag gaaacattgc actacacctt tcgacagggc tacgtacgcc 29520 aggcctgcaa gatctccaac gtggagctct gcaacctggt ctcctacctt ggaattttgc 29580 acgaaaaccg ccttgggcaa aacgtgcttc attccacgct caagggcgag gcgcgccgcg 29640 actacgtccg cgactgcgtt tacttatttc tatgctacac ctggcagacg gccatgggcg 29700 tttggcagca gtgcttggag gagtgcaacc tcaaggagct gcagaaactg ctaaagcaaa 29760 acttgaagga cctatggacg gccttcaacg agcgctccgt ggccgcgcac ctggcggaca 29820 tcattttccc cgaacgcctg cttaaaaccc tgcaacaggg tctgccagac ttcaccagtc 29880 aaagcatgtt gcagaacttt aggaacttta tcctagagcg ctcaggaatc ttgcccgcca 29940 cctgctgtgc acttcctagc gactttgtgc ccattaagta ccgcgaatgc cctccgccgc 30000 tttggggcca ctgctacctt ctgcagctag ccaactacct tgcctaccac tctgacataa 30060 tggaagacgt gagcggtgac ggtctactgg agtgtcactg tcgctgcaac ctatgcaccc 30120 cgcaccgctc cctggtttgc aattcgcagc tgcttaacga aagtcaaatt atcggtacct 30180 ttgagctgca gggtccctcg cctgacgaaa agtccgcggc tccggggttg aaactcactc 30240 cggggctgtg gacgtcggct taccttcgca aatttgtacc tgaggactac cacgcccacg 30300 agattaggtt ctacgaagac caatcccgcc cgcctaatgc ggagcttacc gcctgcgtca 30360 ttacccaggg ccacattctt ggccaattgc aagccatcaa caaagcccgc caagagtttc 30420 tgctacgaaa gggacggggg gtttacttgg acccccagtc cggcgaggag ctcaacccaa 30480 tccccccgcc gccgcagccc tatcagcagc agccgcgggc ccttgcttcc caggatggca 30540 cccaaaaaga agctgcagct gccgccgcca cccacggacg aggaggaata ctgggacagt 30600 caggcagagg aggttttgga cgaggaggag gaggacatga tggaagactg ggagagccta 30660 gacgaggaag cttccgaggt cgaagaggtg tcagacgaaa caccgtcacc ctcggtcgca 30720 ttcccctcgc cggcgcccca gaaatcggca accggttcca gcatggctac aacctccgct 30780 cctcaggcgc cgccggcact gcccgttcgc cgacccaacc gtagatggga caccactgga 30840 accagggccg gtaagtccaa gcagccgccg ccgttagccc aagagcaaca acagcgccaa 30900 ggctaccgct catggcgcgg gcacaagaac gccatagttg cttgcttgca agactgtggg 30960 ggcaacatct ccttcgcccg ccgctttctt ctctaccatc acggcgtggc cttcccccgt 31020 aacatcctgc attactaccg tcatctctac agcccatact gcaccggcgg cagcggcagc 31080 aacagcagcg gccacacaga agcaaaggcg accggatagc aagactctga caaagcccaa 31140 gaaatccaca gcggcggcag cagcaggagg aggagcgctg cgtctggcgc ccaacgaacc 31200 cgtatcgacc cgcgagctta gaaacaggat ttttcccact ctgtatgcta tatttcaaca 31260 gagcaggggc caagaacaag agctgaaaat aaaaaacagg tctctgcgat ccctcacccg 31320 cagctgcctg tatcacaaaa gcgaagatca gcttcggcgc acgctggaag acgcggaggc 31380 tctcttcagt aaatactgcg cgctgactct taaggactag tttcgcgccc tttctcaaat 31440 ttaagcgcga aaactacgtc atctccagcg gccacacccg gcgccagcac ctgttgtcag 31500 cgccattatg agcaaggaaa ttcccacgcc ctacatgtgg agttaccagc cacaaatggg 31560 acttgcggct ggagctgccc aagactactc aacccgaata aactacatga gcgcgggacc 31620 ccacatgata tcccgggtca acggaatacg cgcccaccga aaccgaattc tcctggaaca 31680 ggcggctatt accaccacac ctcgtaataa ccttaatccc cgtagttggc ccgctgccct 31740 ggtgtaccag gaaagtcccg ctcccaccac tgtggtactt cccagagacg cccaggccga 31800 agttcagatg actaactcag gggcgcagct tgcgggcggc tttcgtcaca gggtgcggtc 31860 gcccgggcag ggtataactc acctgacaat cagagggcga ggtattcagc tcaacgacga 31920 gtcggtgagc tcctcgcttg gtctccgtcc ggacgggaca tttcagatcg gcggcgccgg 31980 ccgctcttca ttcacgcctc gtcaggcaat cctaactctg cagacctcgt cctctgagcc 32040 gcgctctgga ggcattggaa ctctgcaatt tattgaggag tttgtgccat cggtctactt 32100 taaccccttc tcgggacctc ccggccacta tccggatcaa tttattccta actttgacgc 32160 ggtaaaggac tcggcggacg gctacgactg aatgttaagt ggagaggcag agcaactgcg 32220 cctgaaacac ctggtccact gtcgccgcca caagtgcttt gcccgcgact ccggtgagtt 32280 ttgctacttt gaattgcccg aggatcatat cgagggcccg gcgcacggcg tccggcttac 32340 cgcccaggga gagcttgccc gtagcctgat tcgggagttt acccagcgcc ccctgctagt 32400 tgagcgggac aggggaccct gtgttctcac tgtgatttgc aactgtccta accctggatt 32460 acatcaagat cctctagtta atgtcaggtc gcctaagtcg attaactaga gtacccgggg 32520 atcttattcc ctttaactaa taaaaaaaaa taataaagca tcacttactt aaaatcagtt 32580 agcaaatttc tgtccagttt attcagcagc acctccttgc cctcctccca gctctggtat 32640 tgcagcttcc tcctggctgc aaactttctc cacaatctaa atggaatgtc agtttcctcc 32700 tgttcctgtc catccgcacc cactatcttc atgttgttgc agatgaagcg cgcaagaccg 32760 tctgaagata ccttcaaccc cgtgtatcca tatgacacgg aaaccggtcc tccaactgtg 32820 ccttttctta ctcctccctt tgtatccccc aatgggtttc aagagagtcc ccctggggta 32880 ctctctttgc gcctatccga acctctagtt acctccaatg gcatgcttgc gctcaaaatg 32940 ggcaacggcc tctctctgga cgaggccggc aaccttacct cccaaaatgt aaccactgtg 33000 agcccacctc tcaaaaaaac caagtcaaac ataaacctgg aaatatctgc acccctcaca 33060 gttacctcag aagccctaac tgtggctgcc gccgcacctc taatggtcgc gggcaacaca 33120 ctcaccatgc aatcacaggc cccgctaacc gtgcacgact ccaaacttag cattgccacc 33180 caaggacccc tcacagtgtc agaaggaaag ctagccctgc aaacatcagg ccccctcacc 33240 accaccgata gcagtaccct tactatcact gcctcacccc ctctaactac tgccactggt 33300 agcttgggca ttgacttgaa agagcccatt tatacacaaa atggaaaact aggactaaag 33360 tacggggctc ctttgcatgt aacagacgac ctaaacactt tgaccgtagc aactggtcca 33420 ggtgtgacta ttaataatac ttccttgcaa actaaagtta ctggagcctt gggttttgat 33480 tcacaaggca atatgcaact taatgtagca ggaggactaa ggattgattc tcaaaacaga 33540 cgccttatac ttgatgttag ttatccgttt gatgctcaaa accaactaaa tctaagacta 33600 ggacagggcc ctctttttat aaactcagcc cacaacttgg atattaacta caacaaaggc 33660 ctttacttgt ttacagcttc aaacaattcc aaaaagcttg aggttaacct aagcactgcc 33720 aaggggttga tgtttgacgc tacagccata gccattaatg caggagatgg gcttgaattt 33780 ggttcaccta atgcaccaaa cacaaatccc ctcaaaacaa aaattggcca tggcctagaa 33840 tttgattcaa acaaggctat ggttcctaaa ctaggaactg gccttagttt tgacagcaca 33900 ggtgccatta cagtaggaaa caaaaataat gataagctaa ctttgtggac cggaataaac 33960 cctccaccta actgtcaaat tgtggaaaac actaatacaa atgatggcaa acttacttta 34020 gtattagtaa aaaatggagg gcttgttaat ggctacgtgt ctctagttgg tgtatcagac 34080 actgtgaacc aaatgttcac acaaaagaca gcaaacatcc aattaagatt atattttgac 34140 tcttctggaa atctattaac tgaggaatca gacttaaaaa ttccacttaa aaataaatct 34200 tctacagcga ccagtgaaac tgtagccagc agcaaagcct ttatgccaag tactacagct 34260 tatcccttca acaccactac tagggatagt gaaaactaca ttcatggaat atgttactac 34320 atgactagtt atgatagaag tctatttccc ttgaacattt ctataatgct aaacagccgt 34380 atgatttctt ccaatgttgc ctatgccata caatttgaat ggaatctaaa tgcaagtgaa 34440 tctccagaaa gcaacatagc tacgctgacc acatccccct ttttcttttc ttacattaca 34500 gaagacgaca actaaagaat cgtttgtgtt atgtttcaac gtgtttattt ttcaattgca 34560 gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata gcttatacag 34620 atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac ctccctccca 34680 acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt 34740 aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc 34800 agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg 34860 agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc 34920 ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc 34980 gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc 35040 ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg cacagcagcg 35100 caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa 35160 aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg 35220 gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat 35280 aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg 35340 attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc 35400 tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc 35460 atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca 35520 cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa caacccattc 35580 ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat 35640 tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtttc 35700 tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca accgagatcg 35760 tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctgaagcaaa 35820 accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta gatcgctctg 35880 tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg gcttcgggtt 35940 ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca gaataagcca 36000 cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg ggaagagctg 36060 gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa atgaagatct 36120 attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa agaacagata 36180 atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct cacgtccaag 36240 tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc agcaccttca 36300 accatgccca aataattctc atctcgccac cttctcaata tatctctaag caaatcccga 36360 atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt cagcctcaag 36420 cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga ttcaaaagcg 36480 gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc tgaacataat 36540 cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg acaaaagaac 36600 ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc ccgatgtaag 36660 cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca ggcaaagcct 36720 cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag gtaagctccg 36780 gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt ttctgcataa 36840 acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta caacaggaaa 36900 aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt aaaaaaactg 36960 gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag tcataatgta 37020 agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc gaccgaaata 37080 gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca taggaggtat 37140 aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct gcctaggcaa 37200 aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag ccataacagt 37260 cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct 37320 caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa 37380 tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca 37440 gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta 37500 cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa 37560 aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca 37620 ttatcatatt ggcttcaatc caaaataagg tatattattg atgatg 37666 <210> 25 <211> 34727 <212> DNA <213> Artificial Sequence <220> <223> #2 vector <400> 25 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct 960 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 1020 agacaccggg accgatccag cctccgattt gccaccatgt ttgtgttcct ggtgctgctg 1080 ccactggtgt ccagccagtg tgtgaacctg accaccagga cccaacttcc tcctgcctac 1140 accaactcct tcaccagggg agtctactac cctgacaagg tgttcaggtc ctctgtgctg 1200 cacagcaccc aggacctgtt cctgccattc ttcagcaatg tgacctggtt ccatgccatc 1260 catgtgtctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc attcaatgat 1320 ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat ttttggcacc 1380 accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa tgtggtgatt 1440 aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg gagtctacta ccacaagaac 1500 aacaagtcct ggatggagtc tgagttcagg gtctactcct ctgccaacaa ctgtaccttt 1560 gaatatgtga gccaaccatt cctgatggac ttggagggca agcagggcaa cttcaagaac 1620 ctgagggagt ttgtgttcaa gaacattgat ggctacttca agatttacag caaacacaca 1680 ccaatcaacc tggtgaggga cctgccacag ggcttctctg ccttggaacc actggtggac 1740 ctgccaattg gcatcaacat caccaggttc cagaccctgc tggctctgca caggtcctac 1800 ctgacacctg gagactcctc ctctggctgg acagcaggag cagcagccta ctatgtgggc 1860 tacctccaac caaggacctt cctgctgaaa tacaatgaga atggcaccat cacagatgct 1920 gtggactgtg ccctggaccc actgtctgag accaagtgta ccctgaaatc cttcacagtg 1980 gagaagggca tctaccagac cagcaacttc agggtccaac caacagagag cattgtgagg 2040 tttccaaaca tcaccaacct gtgtccattt ggagaggtgt tcaatgccac caggtttgcc 2100 tctgtctatg cctggaacag gaagaggatt agcaactgtg tggctgacta ctctgtgctc 2160 tacaactctg cctccttcag caccttcaag tgttatggag tgagcccaac caaactgaat 2220 gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga ttaggggaga tgaggtgaga 2280 cagattgccc ctggacaaac aggcaagatt gctgactaca actacaaact gcctgatgac 2340 ttcacaggct gtgtgattgc ctggaacagc aacaacctgg acagcaaggt gggaggcaac 2400 tacaactacc tctacagact gttcaggaag agcaacctga aaccatttga gagggacatc 2460 agcacagaga tttaccaggc tggcagcaca ccatgtaatg gagtggaggg cttcaactgt 2520 tactttccac tccaatccta tggcttccaa ccaaccaatg gagtgggcta ccaaccatac 2580 agggtggtgg tgctgtcctt tgaactgctc catgcccctg ccacagtgtg tggaccaaag 2640 aagagcacca acctggtgaa gaacaagtgt gtgaacttca acttcaatgg actgacaggc 2700 acaggagtgc tgacagagag caacaagaag ttcctgccat tccaacagtt tggcagggac 2760 attgctgaca ccacagatgc tgtgagggac ccacagacct tggagattct ggacatcaca 2820 ccatgttcct ttggaggagt gtctgtgatt acacctggca ccaacaccag caaccaggtg 2880 gctgtgctct accaggatgt gaactgtact gaggtgcctg tggctatcca tgctgaccaa 2940 cttacaccaa cctggagggt ctacagcaca ggcagcaatg tgttccagac cagggctggc 3000 tgtctgattg gagcagagca tgtgaacaac tcctatgagt gtgacatccc aattggagca 3060 ggcatctgtg cctcctacca gacccagacc atcctcaggt ctgtggcaag ccagagcatc 3120 attgcctaca caatgagtct gggagcagag aactctgtgg cttacagcaa caacagcatt 3180 gccatcccaa ccaacttcac catctctgtg accacagaga ttctgcctgt gagtatgacc 3240 aagacctctg tggactgtac aatgtatatc tgtggagaca gcacagagtg tagcaacctg 3300 ctgctccaat atggctcctt ctgtacccaa cttaacaggg ctctgacagg cattgctgtg 3360 gaacaggaca agaacaccca ggaggtgttt gcccaggtga agcagattta caagacacct 3420 ccaatcaagg actttggagg cttcaacttc agccagattc tgcctgaccc aagcaagcca 3480 agcaagaggt ccttcattga ggacctgctg ttcaacaagg tgaccctggc tgatgctggc 3540 ttcatcaagc aatatggaga ctgtctggga gacattgctg ccagggacct gatttgtgcc 3600 cagaagttca atggactgac agtgctgcct ccactgctga cagatgagat gattgcccaa 3660 tacacctctg ccctgctggc tggcaccatc acctctggct ggacctttgg agcaggagca 3720 gccctccaaa tcccatttgc tatgcagatg gcttacaggt tcaatggcat tggagtgacc 3780 cagaatgtgc tctatgagaa ccagaaactg attgccaacc agttcaactc tgccattggc 3840 aagattcagg actccctgtc cagcacagcc tctgccctgg gcaaactcca agatgtggtg 3900 aaccagaatg cccaggctct gaacaccctg gtgaagcaac tttccagcaa ctttggagcc 3960 atctcctctg tgctgaatga catcctgagc agactggaca aggtggaggc tgaggtccag 4020 attgacagac tgattacagg cagactccaa tccctccaaa cctatgtgac ccaacaactt 4080 atcagggctg ctgagattag ggcatctgcc aacctggctg ccaccaagat gagtgagtgt 4140 gtgctgggac aaagcaagag ggtggacttc tgtggcaagg gctaccacct gatgagtttt 4200 ccacagtctg cccctcatgg agtggtgttc ctgcatgtga cctatgtgcc tgcccaggag 4260 aagaacttca ccacagcccc tgccatctgc catgatggca aggctcactt tccaagggag 4320 ggagtgtttg tgagcaatgg cacccactgg tttgtgaccc agaggaactt ctatgaacca 4380 cagattatca ccacagacaa cacctttgtg tctggcaact gtgatgtggt gattggcatt 4440 gtgaacaaca cagtctatga cccactccaa cctgaactgg actccttcaa ggaggaactg 4500 gacaaatact tcaagaacca caccagccct gatgtggacc tgggagacat ctctggcatc 4560 aatgcctctg tggtgaacat ccagaaggag attgacagac tgaatgaggt ggctaagaac 4620 ctgaatgagt ccctgattga cctccaagaa ctgggcaaat atgaacaata catcaagtgg 4680 ccatgaaaat tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 4740 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 4800 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4860 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 4920 cgcggatctg ggcgtggtta agggtgggaa agaatatata aggtgggggt cttatgtagt 4980 tttgtatctg ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca 5040 ttgtgagctc atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga 5100 tgggctccag cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg 5160 agaccgtgtc tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag 5220 ccaccgcccg cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag 5280 cttcccgttc atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt 5340 tgacccggga acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg 5400 ccctgaaggc ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg 5460 tttggatttg gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt 5520 aggcccggga ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt 5580 aaaggtgact ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc 5640 accactgcag agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc 5700 gctgggcgtg gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct 5760 tggtgtaagt gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat 5820 gcatcttgga ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca 5880 tgttgtgcag aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct 5940 tagaaggaaa tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc 6000 attcgtccat aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg 6060 gatcactaac gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc 6120 gcgggcggag ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac 6180 cctcacagat ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg 6240 gggcgatgaa gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc 6300 tgagcagctg cgacttaccg cagccggtgg gcccgtaaat cacacctatt accggctgca 6360 actggtagtt aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa 6420 gcatgtccct gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca 6480 gcgatagcag ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag 6540 gcatgctttt gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct 6600 ctacggcatc tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta 6660 cggcagtagt cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt 6720 cctcgtcagc gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag 6780 ggtgcgcttg aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc 6840 ggccaggtag catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc 6900 gcgcagcttg cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta 6960 gagcttgggc gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca 7020 gacggtctcg cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt 7080 tcccccatgc tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc 7140 ggtgacgaaa aggctgtccg tgtccccgta tacagacttg agaggcctgt cctcgagcgg 7200 tgttccgcgg tcctcctcgt atagaaactc ggaccactct gagacaaagg ctcgcgtcca 7260 ggccagcacg aaggaggcta agtgggaggg gtagcggtcg ttgtccacta gggggtccac 7320 tcgctccagg gtgtgaagac acatgtcgcc ctcttcggca tcaaggaagg tgattggttt 7380 gtaggtgtag gccacgtgac cgggtgttcc tgaagggggg ctataaaagg gggtgggggc 7440 gcgttcgtcc tcactctctt ccgcatcgct gtctgcgagg gccagctgtt ggggtgagta 7500 ctccctctga aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga 7560 ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcat ccatctggtc 7620 agaaaagaca atctttttgt tgtcaagctt ggtggcaaac gacccgtaga gggcgttgga 7680 cagcaacttg gcgatggagc gcagggtttg gtttttgtcg cgatcggcgc gctccttggc 7740 cgcgatgttt agctgcacgt attcgcgcgc aacgcaccgc cattcgggaa agacggtggt 7800 gcgctcgtcg ggcaccaggt gcacgcgcca accgcggttg tgcagggtga caaggtcaac 7860 gctggtggct acctctccgc gtaggcgctc gttggtccag cagaggcggc cgcccttgcg 7920 cgagcagaat ggcggtaggg ggtctagctg cgtctcgtcc ggggggtctg cgtccacggt 7980 aaagaccccg ggcagcaggc gcgcgtcgaa gtagtctatc ttgcatcctt gcaagtctag 8040 cgcctgctgc catgcgcggg cggcaagcgc gcgctcgtat gggttgagtg ggggacccca 8100 tggcatgggg tgggtgagcg cggaggcgta catgccgcaa atgtcgtaaa cgtagagggg 8160 ctctctgagt attccaagat atgtagggta gcatcttcca ccgcggatgc tggcgcgcac 8220 gtaatcgtat agttcgtgcg agggagcgag gaggtcggga ccgaggttgc tacgggcggg 8280 ctgctctgct cggaagacta tctgcctgaa gatggcatgt gagttggatg atatggttgg 8340 acgctggaag acgttgaagc tggcgtctgt gagacctacc gcgtcacgca cgaaggaggc 8400 gtaggagtcg cgcagcttgt tgaccagctc ggcggtgacc tgcacgtcta gggcgcagta 8460 gtccagggtt tccttgatga tgtcatactt atcctgtccc ttttttttcc acagctcgcg 8520 gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc cgtcggcctc 8580 cgaacggtaa gagcctagca tgtagaactg gttgacggcc tggtaggcgc agcatccctt 8640 ttctacgggt agcgcgtatg cctgcgcggc cttccggagc gaggtgtggg tgagcgcaaa 8700 ggtgtccctg accatgactt tgaggtactg gtatttgaag tcagtgtcgt cgcatccgcc 8760 ctgctcccag agcaaaaagt ccgtgcgctt tttggaacgc ggatttggca gggcgaaggt 8820 gacatcgttg aagagtatct ttcccgcgcg aggcataaag ttgcgtgtga tgcggaaggg 8880 tcccggcacc tcggaacggt tgttaattac ctgggcggcg agcacgatct cgtcaaagcc 8940 gttgatgttg tggcccacaa tgtaaagttc caagaagcgc gggatgccct tgatggaagg 9000 caatttttta agttcctcgt aggtgagctc ttcaggggag ctgagcccgt gctctgaaag 9060 ggcccagtct gcaagatgag ggttggaagc gacgaatgag ctccacaggt cacgggccat 9120 tagcatttgc aggtggtcgc gaaaggtcct aaactggcga cctatggcca ttttttctgg 9180 ggtgatgcag tagaaggtaa gcgggtcttg ttcccagcgg tcccatccaa ggttcgcggc 9240 taggtctcgc gcggcagtca ctagaggctc atctccgccg aacttcatga ccagcatgaa 9300 gggcacgagc tgcttcccaa aggcccccat ccaagtatag gtctctacat cgtaggtgac 9360 aaagagacgc tcggtgcgag gatgcgagcc gatcgggaag aactggatct cccgccacca 9420 attggaggag tggctattga tgtggtgaaa gtagaagtcc ctgcgacggg ccgaacactc 9480 gtgctggctt ttgtaaaaac gtgcgcagta ctggcagcgg tgcacgggct gtacatcctg 9540 cacgaggttg acctgacgac cgcgcacaag gaagcagagt gggaatttga gcccctcgcc 9600 tggcgggttt ggctggtggt cttctacttc ggctgcttgt ccttgaccgt ctggctgctc 9660 gaggggagtt acggtggatc ggaccaccac gccgcgcgag cccaaagtcc agatgtccgc 9720 gcgcggcggt cggagcttga tgacaacatc gcgcagatgg gagctgtcca tggtctggag 9780 ctcccgcggc gtcaggtcag gcgggagctc ctgcaggttt acctcgcata gacgggtcag 9840 ggcgcgggct agatccaggt gatacctaat ttccaggggc tggttggtgg cggcgtcgat 9900 ggcttgcaag aggccgcatc cccgcggcgc gactacggta ccgcgcggcg ggcggtgggc 9960 cgcgggggtg tccttggatg atgcatctaa aagcggtgac gcgggcgagc ccccggaggt 10020 agggggggct ccggacccgc cgggagaggg ggcaggggca cgtcggcgcc gcgcgcgggc 10080 aggagctggt gctgcgcgcg taggttgctg gcgaacgcga cgacgcggcg gttgatctcc 10140 tgaatctggc gcctctgcgt gaagacgacg ggcccggtga gcttgaacct gaaagagagt 10200 tcgacagaat caatttcggt gtcgttgacg gcggcctggc gcaaaatctc ctgcacgtct 10260 cctgagttgt cttgataggc gatctcggcc atgaactgct cgatctcttc ctcctggaga 10320 tctccgcgtc cggctcgctc cacggtggcg gcgaggtcgt tggaaatgcg ggccatgagc 10380 tgcgagaagg cgttgaggcc tccctcgttc cagacgcggc tgtagaccac gcccccttcg 10440 gcatcgcggg cgcgcatgac cacctgcgcg agattgagct ccacgtgccg ggcgaagacg 10500 gcgtagtttc gcaggcgctg aaagaggtag ttgagggtgg tggcggtgtg ttctgccacg 10560 aagaagtaca taacccagcg tcgcaacgtg gattcgttga tatcccccaa ggcctcaagg 10620 cgctccatgg cctcgtagaa gtccacggcg aagttgaaaa actgggagtt gcgcgccgac 10680 acggttaact cctcctccag aagacggatg agctcggcga cagtgtcgcg cacctcgcgc 10740 tcaaaggcta caggggcctc ttcttcttct tcaatctcct cttccataag ggcctcccct 10800 tcttcttctt ctggcggcgg tgggggaggg gggacacggc ggcgacgacg gcgcaccggg 10860 aggcggtcga caaagcgctc gatcatctcc ccgcggcgac ggcgcatggt ctcggtgacg 10920 gcgcggccgt tctcgcgggg gcgcagttgg aagacgccgc ccgtcatgtc ccggttatgg 10980 gttggcgggg ggctgccatg cggcagggat acggcgctaa cgatgcatct caacaattgt 11040 tgtgtaggta ctccgccgcc gagggacctg agcgagtccg catcgaccgg atcggaaaac 11100 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc 11160 ggcagcgggc ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag 11220 taggcggtct tgagacggcg gatggtcgac agaagcacca tgtccttggg tccggcctgc 11280 tgaatgcgca ggcggtcggc catgccccag gcttcgtttt gacatcggcg caggtctttg 11340 tagtagtctt gcatgagcct ttctaccggc acttcttctt ctccttcctc ttgtcctgca 11400 tctcttgcat ctatcgctgc ggcggcggcg gagtttggcc gtaggtggcg ccctcttcct 11460 cccatgcgtg tgaccccgaa gcccctcatc ggctgaagca gggctaggtc ggcgacaacg 11520 cgctcggcta atatggcctg ctgcacctgc gtgagggtag actggaagtc atccatgtcc 11580 acaaagcggt ggtatgcgcc cgtgttgatg gtgtaagtgc agttggccat aacggaccag 11640 ttaacggtct ggtgacccgg ctgcgagagc tcggtgtacc tgagacgcga gtaagccctc 11700 gagtcaaata cgtagtcgtt gcaagtccgc accaggtact ggtatcccac caaaaagtgc 11760 ggcggcggct ggcggtagag gggccagcgt agggtggccg gggctccggg ggcgagatct 11820 tccaacataa ggcgatgata tccgtagatg tacctggaca tccaggtgat gccggcggcg 11880 gtggtggagg cgcgcggaaa gtcgcggacg cggttccaga tgttgcgcag cggcaaaaag 11940 tgctccatgg tcgggacgct ctggccggtc aggcgcgcgc aatcgttgac gctctagcgt 12000 gcaaaaggag agcctgtaag cgggcactct tccgtggtct ggtggataaa ttcgcaaggg 12060 tatcatggcg gacgaccggg gttcgagccc cgtatccggc cgtccgccgt gatccatgcg 12120 gttaccgccc gcgtgtcgaa cccaggtgtg cgacgtcaga caacggggga gtgctccttt 12180 tggcttcctt ccaggcgcgg cggctgctgc gctagctttt ttggccactg gccgcgcgca 12240 gcgtaagcgg ttaggctgga aagcgaaagc attaagtggc tcgctccctg tagccggagg 12300 gttattttcc aagggttgag tcgcgggacc cccggttcga gtctcggacc ggccggactg 12360 cggcgaacgg gggtttgcct ccccgtcatg caagaccccg cttgcaaatt cctccggaaa 12420 cagggacgag cccctttttt gcttttccca gatgcatccg gtgctgcggc agatgcgccc 12480 ccctcctcag cagcggcaag agcaagagca gcggcagaca tgcagggcac cctcccctcc 12540 tcctaccgcg tcaggagggg cgacatccgc ggttgacgcg gcagcagatg gtgattacga 12600 acccccgcgg cgccgggccc ggcactacct ggacttggag gagggcgagg gcctggcgcg 12660 gctaggagcg ccctctcctg agcggcaccc aagggtgcag ctgaagcgtg atacgcgtga 12720 ggcgtacgtg ccgcggcaga acctgtttcg cgaccgcgag ggagaggagc ccgaggagat 12780 gcgggatcga aagttccacg cagggcgcga gctgcggcat ggcctgaatc gcgagcggtt 12840 gctgcgcgag gaggactttg agcccgacgc gcgaaccggg attagtcccg cgcgcgcaca 12900 cgtggcggcc gccgacctgg taaccgcata cgagcagacg gtgaaccagg agattaactt 12960 tcaaaaaagc tttaacaacc acgtgcgtac gcttgtggcg cgcgaggagg tggctatagg 13020 actgatgcat ctgtgggact ttgtaagcgc gctggagcaa aacccaaata gcaagccgct 13080 catggcgcag ctgttcctta tagtgcagca cagcagggac aacgaggcat tcagggatgc 13140 gctgctaaac atagtagagc ccgagggccg ctggctgctc gatttgataa acatcctgca 13200 gagcatagtg gtgcaggagc gcagcttgag cctggctgac aaggtggccg ccatcaacta 13260 ttccatgctt agcctgggca agttttacgc ccgcaagata taccataccc cttacgttcc 13320 catagacaag gaggtaaaga tcgaggggtt ctacatgcgc atggcgctga aggtgcttac 13380 cttgagcgac gacctgggcg tttatcgcaa cgagcgcatc cacaaggccg tgagcgtgag 13440 ccggcggcgc gagctcagcg accgcgagct gatgcacagc ctgcaaaggg ccctggctgg 13500 cacgggcagc ggcgatagag aggccgagtc ctactttgac gcgggcgctg acctgcgctg 13560 ggccccaagc cgacgcgccc tggaggcagc tggggccgga cctgggctgg cggtggcacc 13620 cgcgcgcgct ggcaacgtcg gcggcgtgga ggaatatgac gaggacgatg agtacgagcc 13680 agaggacggc gagtactaag cggtgatgtt tctgatcaga tgatgcaaga cgcaacggac 13740 ccggcggtgc gggcggcgct gcagagccag ccgtccggcc ttaactccac ggacgactgg 13800 cgccaggtca tggaccgcat catgtcgctg actgcgcgca atcctgacgc gttccggcag 13860 cagccgcagg ccaaccggct ctccgcaatt ctggaagcgg tggtcccggc gcgcgcaaac 13920 cccacgcacg agaaggtgct ggcgatcgta aacgcgctgg ccgaaaacag ggccatccgg 13980 cccgacgagg ccggcctggt ctacgacgcg ctgcttcagc gcgtggctcg ttacaacagc 14040 ggcaacgtgc agaccaacct ggaccggctg gtgggggatg tgcgcgaggc cgtggcgcag 14100 cgtgagcgcg cgcagcagca gggcaacctg ggctccatgg ttgcactaaa cgccttcctg 14160 agtacacagc ccgccaacgt gccgcgggga caggaggact acaccaactt tgtgagcgca 14220 ctgcggctaa tggtgactga gacaccgcaa agtgaggtgt accagtctgg gccagactat 14280 tttttccaga ccagtagaca aggcctgcag accgtaaacc tgagccaggc tttcaaaaac 14340 ttgcaggggc tgtggggggt gcgggctccc acaggcgacc gcgcgaccgt gtctagcttg 14400 ctgacgccca actcgcgcct gttgctgctg ctaatagcgc ccttcacgga cagtggcagc 14460 gtgtcccggg acacatacct aggtcacttg ctgacactgt accgcgaggc cataggtcag 14520 gcgcatgtgg acgagcatac tttccaggag attacaagtg tcagccgcgc gctggggcag 14580 gaggacacgg gcagcctgga ggcaacccta aactacctgc tgaccaaccg gcggcagaag 14640 atcccctcgt tgcacagttt aaacagcgag gaggagcgca ttttgcgcta cgtgcagcag 14700 agcgtgagcc ttaacctgat gcgcgacggg gtaacgccca gcgtggcgct ggacatgacc 14760 gcgcgcaaca tggaaccggg catgtatgcc tcaaaccggc cgtttatcaa ccgcctaatg 14820 gactacttgc atcgcgcggc cgccgtgaac cccgagtatt tcaccaatgc catcttgaac 14880 ccgcactggc taccgccccc tggtttctac accgggggat tcgaggtgcc cgagggtaac 14940 gatggattcc tctgggacga catagacgac agcgtgtttt ccccgcaacc gcagaccctg 15000 ctagagttgc aacagcgcga gcaggcagag gcggcgctgc gaaaggaaag cttccgcagg 15060 ccaagcagct tgtccgatct aggcgctgcg gccccgcggt cagatgctag tagcccattt 15120 ccaagcttga tagggtctct taccagcact cgcaccaccc gcccgcgcct gctgggcgag 15180 gaggagtacc taaacaactc gctgctgcag ccgcagcgcg aaaaaaacct gcctccggca 15240 tttcccaaca acgggataga gagcctagtg gacaagatga gtagatggaa gacgtacgcg 15300 caggagcaca gggacgtgcc aggcccgcgc ccgcccaccc gtcgtcaaag gcacgaccgt 15360 cagcggggtc tggtgtggga ggacgatgac tcggcagacg acagcagcgt cctggatttg 15420 ggagggagtg gcaacccgtt tgcgcacctt cgccccaggc tggggagaat gttttaaaaa 15480 aaaaaaagca tgatgcaaaa taaaaaactc accaaggcca tggcaccgag cgttggtttt 15540 cttgtattcc ccttagtatg cggcgcgcgg cgatgtatga ggaaggtcct cctccctcct 15600 acgagagtgt ggtgagcgcg gcgccagtgg cggcggcgct gggttctccc ttcgatgctc 15660 ccctggaccc gccgtttgtg cctccgcggt acctgcggcc taccgggggg agaaacagca 15720 tccgttactc tgagttggca cccctattcg acaccacccg tgtgtacctg gtggacaaca 15780 agtcaacgga tgtggcatcc ctgaactacc agaacgacca cagcaacttt ctgaccacgg 15840 tcattcaaaa caatgactac agcccggggg aggcaagcac acagaccatc aatcttgacg 15900 accggtcgca ctggggcggc gacctgaaaa ccatcctgca taccaacatg ccaaatgtga 15960 acgagttcat gtttaccaat aagtttaagg cgcgggtgat ggtgtcgcgc ttgcctacta 16020 aggacaatca ggtggagctg aaatacgagt gggtggagtt cacgctgccc gagggcaact 16080 actccgagac catgaccata gaccttatga acaacgcgat cgtggagcac tacttgaaag 16140 tgggcagaca gaacggggtt ctggaaagcg acatcggggt aaagtttgac acccgcaact 16200 tcagactggg gtttgacccc gtcactggtc ttgtcatgcc tggggtatat acaaacgaag 16260 ccttccatcc agacatcatt ttgctgccag gatgcggggt ggacttcacc cacagccgcc 16320 tgagcaactt gttgggcatc cgcaagcggc aacccttcca ggagggcttt aggatcacct 16380 acgatgatct ggagggtggt aacattcccg cactgttgga tgtggacgcc taccaggcga 16440 gcttgaaaga tgacaccgaa cagggcgggg gtggcgcagg cggcagcaac agcagtggca 16500 gcggcgcgga agagaactcc aacgcggcag ccgcggcaat gcagccggtg gaggacatga 16560 acgatcatgc cattcgcggc gacacctttg ccacacgggc tgaggagaag cgcgctgagg 16620 ccgaagcagc ggccgaagct gccgcccccg ctgcgcaacc cgaggtcgag aagcctcaga 16680 agaaaccggt gatcaaaccc ctgacagagg acagcaagaa acgcagttac aacctaataa 16740 gcaatgacag caccttcacc cagtaccgca gctggtacct tgcatacaac tacggcgacc 16800 ctcagaccgg aatccgctca tggaccctgc tttgcactcc tgacgtaacc tgcggctcgg 16860 agcaggtcta ctggtcgttg ccagacatga tgcaagaccc cgtgaccttc cgctccacgc 16920 gccagatcag caactttccg gtggtgggcg ccgagctgtt gcccgtgcac tccaagagct 16980 tctacaacga ccaggccgtc tactcccaac tcatccgcca gtttacctct ctgacccacg 17040 tgttcaatcg ctttcccgag aaccagattt tggcgcgccc gccagccccc accatcacca 17100 ccgtcagtga aaacgttcct gctctcacag atcacgggac gctaccgctg cgcaacagca 17160 tcggaggagt ccagcgagtg accattactg acgccagacg ccgcacctgc ccctacgttt 17220 acaaggccct gggcatagtc tcgccgcgcg tcctatcgag ccgcactttt tgagcaagca 17280 tgtccatcct tatatcgccc agcaataaca caggctgggg cctgcgcttc ccaagcaaga 17340 tgtttggcgg ggccaagaag cgctccgacc aacacccagt gcgcgtgcgc gggcactacc 17400 gcgcgccctg gggcgcgcac aaacgcggcc gcactgggcg caccaccgtc gatgacgcca 17460 tcgacgcggt ggtggaggag gcgcgcaact acacgcccac gccgccacca gtgtccacag 17520 tggacgcggc cattcagacc gtggtgcgcg gagcccggcg ctatgctaaa atgaagagac 17580 ggcggaggcg cgtagcacgt cgccaccgcc gccgacccgg cactgccgcc caacgcgcgg 17640 cggcggccct gcttaaccgc gcacgtcgca ccggccgacg ggcggccatg cgggccgctc 17700 gaaggctggc cgcgggtatt gtcactgtgc cccccaggtc caggcgacga gcggccgccg 17760 cagcagccgc ggccattagt gctatgactc agggtcgcag gggcaacgtg tattgggtgc 17820 gcgactcggt tagcggcctg cgcgtgcccg tgcgcacccg ccccccgcgc aactagattg 17880 caagaaaaaa ctacttagac tcgtactgtt gtatgtatcc agcggcggcg gcgcgcaacg 17940 aagctatgtc caagcgcaaa atcaaagaag agatgctcca ggtcatcgcg ccggagatct 18000 atggcccccc gaagaaggaa gagcaggatt acaagccccg aaagctaaag cgggtcaaaa 18060 agaaaaagaa agatgatgat gatgaacttg acgacgaggt ggaactgctg cacgctaccg 18120 cgcccaggcg acgggtacag tggaaaggtc gacgcgtaaa acgtgttttg cgacccggca 18180 ccaccgtagt ctttacgccc ggtgagcgct ccacccgcac ctacaagcgc gtgtatgatg 18240 aggtgtacgg cgacgaggac ctgcttgagc aggccaacga gcgcctcggg gagtttgcct 18300 acggaaagcg gcataaggac atgctggcgt tgccgctgga cgagggcaac ccaacaccta 18360 gcctaaagcc cgtaacactg cagcaggtgc tgcccgcgct tgcaccgtcc gaagaaaagc 18420 gcggcctaaa gcgcgagtct ggtgacttgg cacccaccgt gcagctgatg gtacccaagc 18480 gccagcgact ggaagatgtc ttggaaaaaa tgaccgtgga acctgggctg gagcccgagg 18540 tccgcgtgcg gccaatcaag caggtggcgc cgggactggg cgtgcagacc gtggacgttc 18600 agatacccac taccagtagc accagtattg ccaccgccac agagggcatg gagacacaaa 18660 cgtccccggt tgcctcagcg gtggcggatg ccgcggtgca ggcggtcgct gcggccgcgt 18720 ccaagacctc tacggaggtg caaacggacc cgtggatgtt tcgcgtttca gccccccggc 18780 gcccgcgccg ttcgaggaag tacggcgccg ccagcgcgct actgcccgaa tatgccctac 18840 atccttccat tgcgcctacc cccggctatc gtggctacac ctaccgcccc agaagacgag 18900 caactacccg acgccgaacc accactggaa cccgccgccg ccgtcgccgt cgccagcccg 18960 tgctggcccc gatttccgtg cgcagggtgg ctcgcgaagg aggcaggacc ctggtgctgc 19020 caacagcgcg ctaccacccc agcatcgttt aaaagccggt ctttgtggtt cttgcagata 19080 tggccctcac ctgccgcctc cgtttcccgg tgccgggatt ccgaggaaga atgcaccgta 19140 ggaggggcat ggccggccac ggcctgacgg gcggcatgcg tcgtgcgcac caccggcggc 19200 ggcgcgcgtc gcaccgtcgc atgcgcggcg gtatcctgcc cctccttatt ccactgatcg 19260 ccgcggcgat tggcgccgtg cccggaattg catccgtggc cttgcaggcg cagagacact 19320 gattaaaaac aagttgcatg tggaaaaatc aaaataaaaa gtctggactc tcacgctcgc 19380 ttggtcctgt aactattttg tagaatggaa gacatcaact ttgcgtctct ggccccgcga 19440 cacggctcgc gcccgttcat gggaaactgg caagatatcg gcaccagcaa tatgagcggt 19500 ggcgccttca gctggggctc gctgtggagc ggcattaaaa atttcggttc caccgttaag 19560 aactatggca gcaaggcctg gaacagcagc acaggccaga tgctgaggga taagttgaaa 19620 gagcaaaatt tccaacaaaa ggtggtagat ggcctggcct ctggcattag cggggtggtg 19680 gacctggcca accaggcagt gcaaaataag attaacagta agcttgatcc ccgccctccc 19740 gtagaggagc ctccaccggc cgtggagaca gtgtctccag aggggcgtgg cgaaaagcgt 19800 ccgcgccccg acagggaaga aactctggtg acgcaaatag acgagcctcc ctcgtacgag 19860 gaggcactaa agcaaggcct gcccaccacc cgtcccatcg cgcccatggc taccggagtg 19920 ctgggccagc acacacccgt aacgctggac ctgcctcccc ccgccgacac ccagcagaaa 19980 cctgtgctgc caggcccgac cgccgttgtt gtaacccgtc ctagccgcgc gtccctgcgc 20040 cgcgccgcca gcggtccgcg atcgttgcgg cccgtagcca gtggcaactg gcaaagcaca 20100 ctgaacagca tcgtgggtct gggggtgcaa tccctgaagc gccgacgatg cttctgatag 20160 ctaacgtgtc gtatgtgtgt catgtatgcg tccatgtcgc cgccagagga gctgctgagc 20220 cgccgcgcgc ccgctttcca agatggctac cccttcgatg atgccgcagt ggtcttacat 20280 gcacatctcg ggccaggacg cctcggagta cctgagcccc gggctggtgc agtttgcccg 20340 cgccaccgag acgtacttca gcctgaataa caagtttaga aaccccacgg tggcgcctac 20400 gcacgacgtg accacagacc ggtcccagcg tttgacgctg cggttcatcc ctgtggaccg 20460 tgaggatact gcgtactcgt acaaggcgcg gttcacccta gctgtgggtg ataaccgtgt 20520 gctggacatg gcttccacgt actttgacat ccgcggcgtg ctggacaggg gccctacttt 20580 taagccctac tctggcactg cctacaacgc cctggctccc aagggtgccc caaatccttg 20640 cgaatgggat gaagctgcta ctgctcttga aataaaccta gaagaagagg acgatgacaa 20700 cgaagacgaa gtagacgagc aagctgagca gcaaaaaact cacgtatttg ggcaggcgcc 20760 ttattctggt ataaatatta caaaggaggg tattcaaata ggtgtcgaag gtcaaacacc 20820 taaatatgcc gataaaacat ttcaacctga acctcaaata ggagaatctc agtggtacga 20880 aacagaaatt aatcatgcag ctgggagagt cctaaaaaag actaccccaa tgaaaccatg 20940 ttacggttca tatgcaaaac ccacaaatga aaatggaggg caaggcattc ttgtaaagca 21000 acaaaatgga aagctagaaa gtcaagtgga aatgcaattt ttctcaacta ctgaggcagc 21060 cgcaggcaat ggtgataact tgactcctaa agtggtattg tacagtgaag atgtagatat 21120 agaaacccca gacactcata tttcttacat gcccactatt aaggaaggta actcacgaga 21180 actaatgggc caacaatcta tgcccaacag gcctaattac attgctttta gggacaattt 21240 tattggtcta atgtattaca acagcacggg taatatgggt gttctggcgg gccaagcatc 21300 gcagttgaat gctgttgtag atttgcaaga cagaaacaca gagctttcat accagctttt 21360 gcttgattcc attggtgata gaaccaggta cttttctatg tggaatcagg ctgttgacag 21420 ctatgatcca gatgttagaa ttattgaaaa tcatggaact gaagatgaac ttccaaatta 21480 ctgctttcca ctgggaggtg tgattaatac agagactctt accaaggtaa aacctaaaac 21540 aggtcaggaa aatggatggg aaaaagatgc tacagaattt tcagataaaa atgaaataag 21600 agttggaaat aattttgcca tggaaatcaa tctaaatgcc aacctgtgga gaaatttcct 21660 gtactccaac atagcgctgt atttgcccga caagctaaag tacagtcctt ccaacgtaaa 21720 aatttctgat aacccaaaca cctacgacta catgaacaag cgagtggtgg ctcccgggct 21780 agtggactgc tacattaacc ttggagcacg ctggtccctt gactatatgg acaacgtcaa 21840 cccatttaac caccaccgca atgctggcct gcgctaccgc tcaatgttgc tgggcaatgg 21900 tcgctatgtg cccttccaca tccaggtgcc tcagaagttc tttgccatta aaaacctcct 21960 tctcctgccg ggctcataca cctacgagtg gaacttcagg aaggatgtta acatggttct 22020 gcagagctcc ctaggaaatg acctaagggt tgacggagcc agcattaagt ttgatagcat 22080 ttgcctttac gccaccttct tccccatggc ccacaacacc gcctccacgc ttgaggccat 22140 gcttagaaac gacaccaacg accagtcctt taacgactat ctctccgccg ccaacatgct 22200 ctaccctata cccgccaacg ctaccaacgt gcccatatcc atcccctccc gcaactgggc 22260 ggctttccgc ggctgggcct tcacgcgcct taagactaag gaaaccccat cactgggctc 22320 gggctacgac ccttattaca cctactctgg ctctataccc tacctagatg gaacctttta 22380 cctcaaccac acctttaaga aggtggccat tacctttgac tcttctgtca gctggcctgg 22440 caatgaccgc ctgcttaccc ccaacgagtt tgaaattaag cgctcagttg acggggaggg 22500 ttacaacgtt gcccagtgta acatgaccaa agactggttc ctggtacaaa tgctagctaa 22560 ctataacatt ggctaccagg gcttctatat cccagagagc tacaaggacc gcatgtactc 22620 cttctttaga aacttccagc ccatgagccg tcaggtggtg gatgatacta aatacaagga 22680 ctaccaacag gtgggcatcc tacaccaaca caacaactct ggatttgttg gctaccttgc 22740 ccccaccatg cgcgaaggac aggcctaccc tgctaacttc ccctatccgc ttataggcaa 22800 gaccgcagtt gacagcatta cccagaaaaa gtttctttgc gatcgcaccc tttggcgcat 22860 cccattctcc agtaacttta tgtccatggg cgcactcaca gacctgggcc aaaaccttct 22920 ctacgccaac tccgcccacg cgctagacat gacttttgag gtggatccca tggacgagcc 22980 cacccttctt tatgttttgt ttgaagtctt tgacgtggtc cgtgtgcacc agccgcaccg 23040 cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg gccggcaacg ccacaacata 23100 aagaagcaag caacatcaac aacagctgcc gccatgggct ccagtgagca ggaactgaaa 23160 gccattgtca aagatcttgg ttgtgggcca tattttttgg gcacctatga caagcgcttt 23220 ccaggctttg tttctccaca caagctcgcc tgcgccatag tcaatacggc cggtcgcgag 23280 actgggggcg tacactggat ggcctttgcc tggaacccgc actcaaaaac atgctacctc 23340 tttgagccct ttggcttttc tgaccagcga ctcaagcagg tttaccagtt tgagtacgag 23400 tcactcctgc gccgtagcgc cattgcttct tcccccgacc gctgtataac gctggaaaag 23460 tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg gactattctg ctgcatgttt 23520 ctccacgcct ttgccaactg gccccaaact cccatggatc acaaccccac catgaacctt 23580 attaccgggg tacccaactc catgctcaac agtccccagg tacagcccac cctgcgtcgc 23640 aaccaggaac agctctacag cttcctggag cgccactcgc cctacttccg cagccacagt 23700 gcgcagatta ggagcgccac ttctttttgt cacttgaaaa acatgtaaaa ataatgtact 23760 agagacactt tcaataaagg caaatgcttt tatttgtaca ctctcgggtg attatttacc 23820 cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt tctgccgcgc atcgctatgc 23880 gccactggca gggacacgtt gcgatactgg tgtttagtgc tccacttaaa ctcaggcaca 23940 accatccgcg gcagctcggt gaagttttca ctccacaggc tgcgcaccat caccaacgcg 24000 tttagcaggt cgggcgccga tatcttgaag tcgcagttgg ggcctccgcc ctgcgcgcgc 24060 gagttgcgat acacagggtt gcagcactgg aacactatca gcgccgggtg gtgcacgctg 24120 gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt cctccgcgtt gctcagggcg 24180 aacggagtca actttggtag ctgccttccc aaaaagggcg cgtgcccagg ctttgagttg 24240 cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg tctgggcgtt aggatacagc 24300 gcctgcataa aagccttgat ctgcttaaaa gccacctgag cctttgcgcc ttcagagaag 24360 aacatgccgc aagacttgcc ggaaaactga ttggccggac aggccgcgtc gtgcacgcag 24420 caccttgcgt cggtgttgga gatctgcacc acatttcggc cccaccggtt cttcacgatc 24480 ttggccttgc tagactgctc cttcagcgcg cgctgcccgt tttcgctcgt cacatccatt 24540 tcaatcacgt gctccttatt tatcataatg cttccgtgta gacacttaag ctcgccttcg 24600 atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg gctcgtgatg cttgtaggtc 24660 acctctgcaa acgactgcag gtacgcctgc aggaatcgcc ccatcatcgt cacaaaggtc 24720 ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct cgttcagcca ggtcttgcat 24780 acggccgcca gagcttccac ttggtcaggc agtagtttga agttcgcctt tagatcgtta 24840 tccacgtggt acttgtccat cagcgcgcgc gcagcctcca tgcccttctc ccacgcagac 24900 acgatcggca cactcagcgg gttcatcacc gtaatttcac tttccgcttc gctgggctct 24960 tcctcttcct cttgcgtccg cataccacgc gccactgggt cgtcttcatt cagccgccgc 25020 actgtgcgct tacctccttt gccatgcttg attagcaccg gtgggttgct gaaacccacc 25080 atttgtagcg ccacatcttc tctttcttcc tcgctgtcca cgattacctc tggtgatggc 25140 gggcgctcgg gcttgggaga agggcgcttc tttttcttct tgggcgcaat ggccaaatcc 25200 gccgccgagg tcgatggccg cgggctgggt gtgcgcggca ccagcgcgtc ttgtgatgag 25260 tcttcctcgt cctcggactc gatacgccgc ctcatccgct tttttggggg cgcccgggga 25320 ggcggcggcg acggggacgg ggacgacacg tcctccatgg ttgggggacg tcgcgccgca 25380 ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt cccgactggc catttccttc 25440 tcctataggc agaaaaagat catggagtca gtcgagaaga aggacagcct aaccgccccc 25500 tctgagttcg ccaccaccgc ctccaccgat gccgccaacg cgcctaccac cttccccgtc 25560 gaggcacccc cgcttgagga ggaggaagtg attatcgagc aggacccagg ttttgtaagc 25620 gaagacgacg aggaccgctc agtaccaaca gaggataaaa agcaagacca ggacaacgca 25680 gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc atggcgacta cctagatgtg 25740 ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg ccattatctg cgacgcgttg 25800 caagagcgca gcgatgtgcc cctcgccata gcggatgtca gccttgccta cgaacgccac 25860 ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg gcacatgcga gcccaacccg 25920 cgcctcaact tctaccccgt atttgccgtg ccagaggtgc ttgccaccta tcacatcttt 25980 ttccaaaact gcaagatacc cctatcctgc cgtgccaacc gcagccgagc ggacaagcag 26040 ctggccttgc ggcagggcgc tgtcatacct gatatcgcct cgctcaacga agtgccaaaa 26100 atctttgagg gtcttggacg cgacgagaag cgcgcggcaa acgctctgca acaggaaaac 26160 agcgaaaatg aaagtcactc tggagtgttg gtggaactcg agggtgacaa cgcgcgccta 26220 gccgtactaa aacgcagcat cgaggtcacc cactttgcct acccggcact taacctaccc 26280 cccaaggtca tgagcacagt catgagtgag ctgatcgtgc gccgtgcgca gcccctggag 26340 agggatgcaa atttgcaaga acaaacagag gagggcctac ccgcagttgg cgacgagcag 26400 ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg aggagcgacg caaactaatg 26460 atggccgcag tgctcgttac cgtggagctt gagtgcatgc agcggttctt tgctgacccg 26520 gagatgcagc gcaagctaga ggaaacattg cactacacct ttcgacaggg ctacgtacgc 26580 caggcctgca agatctccaa cgtggagctc tgcaacctgg tctcctacct tggaattttg 26640 cacgaaaacc gccttgggca aaacgtgctt cattccacgc tcaagggcga ggcgcgccgc 26700 gactacgtcc gcgactgcgt ttacttattt ctatgctaca cctggcagac ggccatgggc 26760 gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc tgcagaaact gctaaagcaa 26820 aacttgaagg acctatggac ggccttcaac gagcgctccg tggccgcgca cctggcggac 26880 atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg gtctgccaga cttcaccagt 26940 caaagcatgt tgcagaactt taggaacttt atcctagagc gctcaggaat cttgcccgcc 27000 acctgctgtg cacttcctag cgactttgtg cccattaagt accgcgaatg ccctccgccg 27060 ctttggggcc actgctacct tctgcagcta gccaactacc ttgcctacca ctctgacata 27120 atggaagacg tgagcggtga cggtctactg gagtgtcact gtcgctgcaa cctatgcacc 27180 ccgcaccgct ccctggtttg caattcgcag ctgcttaacg aaagtcaaat tatcggtacc 27240 tttgagctgc agggtccctc gcctgacgaa aagtccgcgg ctccggggtt gaaactcact 27300 ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac ctgaggacta ccacgcccac 27360 gagattaggt tctacgaaga ccaatcccgc ccgcctaatg cggagcttac cgcctgcgtc 27420 attacccagg gccacattct tggccaattg caagccatca acaaagcccg ccaagagttt 27480 ctgctacgaa agggacgggg ggtttacttg gacccccagt ccggcgagga gctcaaccca 27540 atccccccgc cgccgcagcc ctatcagcag cagccgcggg cccttgcttc ccaggatggc 27600 acccaaaaag aagctgcagc tgccgccgcc acccacggac gaggaggaat actgggacag 27660 tcaggcagag gaggttttgg acgaggagga ggaggacatg atggaagact gggagagcct 27720 agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa acaccgtcac cctcggtcgc 27780 attcccctcg ccggcgcccc agaaatcggc aaccggttcc agcatggcta caacctccgc 27840 tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac cgtagatggg acaccactgg 27900 aaccagggcc ggtaagtcca agcagccgcc gccgttagcc caagagcaac aacagcgcca 27960 aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt gcttgcttgc aagactgtgg 28020 gggcaacatc tccttcgccc gccgctttct tctctaccat cacggcgtgg ccttcccccg 28080 taacatcctg cattactacc gtcatctcta cagcccatac tgcaccggcg gcagcggcag 28140 caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg acaaagccca 28200 agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg cccaacgaac 28260 ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct atatttcaac 28320 agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga tccctcaccc 28380 gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa gacgcggagg 28440 ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc ctttctcaaa 28500 tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca cctgttgtca 28560 gcgccattat gagcaaggaa attcccacgc cctacatgtg gagttaccag ccacaaatgg 28620 gacttgcggc tggagctgcc caagactact caacccgaat aaactacatg agcgcgggac 28680 cccacatgat atcccgggtc aacggaatac gcgcccaccg aaaccgaatt ctcctggaac 28740 aggcggctat taccaccaca cctcgtaata accttaatcc ccgtagttgg cccgctgccc 28800 tggtgtacca ggaaagtccc gctcccacca ctgtggtact tcccagagac gcccaggccg 28860 aagttcagat gactaactca ggggcgcagc ttgcgggcgg ctttcgtcac agggtgcggt 28920 cgcccgggca gggtataact cacctgacaa tcagagggcg aggtattcag ctcaacgacg 28980 agtcggtgag ctcctcgctt ggtctccgtc cggacgggac atttcagatc ggcggcgccg 29040 gccgctcttc attcacgcct cgtcaggcaa tcctaactct gcagacctcg tcctctgagc 29100 cgcgctctgg aggcattgga actctgcaat ttattgagga gtttgtgcca tcggtctact 29160 ttaacccctt ctcgggacct cccggccact atccggatca atttattcct aactttgacg 29220 cggtaaagga ctcggcggac ggctacgact gaatgttaag tggagaggca gagcaactgc 29280 gcctgaaaca cctggtccac tgtcgccgcc acaagtgctt tgcccgcgac tccggtgagt 29340 tttgctactt tgaattgccc gaggatcata tcgagggccc ggcgcacggc gtccggctta 29400 ccgcccaggg agagcttgcc cgtagcctga ttcgggagtt tacccagcgc cccctgctag 29460 ttgagcggga caggggaccc tgtgttctca ctgtgatttg caactgtcct aaccctggat 29520 tacatcaaga tcctctagtt aatgtcaggt cgcctaagtc gattaactag agtacccggg 29580 gatcttattc cctttaacta ataaaaaaaa ataataaagc atcacttact taaaatcagt 29640 tagcaaattt ctgtccagtt tattcagcag cacctccttg ccctcctccc agctctggta 29700 ttgcagcttc ctcctggctg caaactttct ccacaatcta aatggaatgt cagtttcctc 29760 ctgttcctgt ccatccgcac ccactatctt catgttgttg cagatgaagc gcgcaagacc 29820 gtctgaagat accttcaacc ccgtgtatcc atatgacacg gaaaccggtc ctccaactgt 29880 gccttttctt actcctccct ttgtatcccc caatgggttt caagagagtc cccctggggt 29940 actctctttg cgcctatccg aacctctagt tacctccaat ggcatgcttg cgctcaaaat 30000 gggcaacggc ctctctctgg acgaggccgg caaccttacc tcccaaaatg taaccactgt 30060 gagcccacct ctcaaaaaaa ccaagtcaaa cataaacctg gaaatatctg cacccctcac 30120 agttacctca gaagccctaa ctgtggctgc cgccgcacct ctaatggtcg cgggcaacac 30180 actcaccatg caatcacagg ccccgctaac cgtgcacgac tccaaactta gcattgccac 30240 ccaaggaccc ctcacagtgt cagaaggaaa gctagccctg caaacatcag gccccctcac 30300 caccaccgat agcagtaccc ttactatcac tgcctcaccc cctctaacta ctgccactgg 30360 tagcttgggc attgacttga aagagcccat ttatacacaa aatggaaaac taggactaaa 30420 gtacggggct cctttgcatg taacagacga cctaaacact ttgaccgtag caactggtcc 30480 aggtgtgact attaataata cttccttgca aactaaagtt actggagcct tgggttttga 30540 ttcacaaggc aatatgcaac ttaatgtagc aggaggacta aggattgatt ctcaaaacag 30600 acgccttata cttgatgtta gttatccgtt tgatgctcaa aaccaactaa atctaagact 30660 aggacagggc cctcttttta taaactcagc ccacaacttg gatattaact acaacaaagg 30720 cctttacttg tttacagctt caaacaattc caaaaagctt gaggttaacc taagcactgc 30780 caaggggttg atgtttgacg ctacagccat agccattaat gcaggagatg ggcttgaatt 30840 tggttcacct aatgcaccaa acacaaatcc cctcaaaaca aaaattggcc atggcctaga 30900 atttgattca aacaaggcta tggttcctaa actaggaact ggccttagtt ttgacagcac 30960 aggtgccatt acagtaggaa acaaaaataa tgataagcta actttgtgga ccggaataaa 31020 ccctccacct aactgtcaaa ttgtggaaaa cactaataca aatgatggca aacttacttt 31080 agtattagta aaaaatggag ggcttgttaa tggctacgtg tctctagttg gtgtatcaga 31140 cactgtgaac caaatgttca cacaaaagac agcaaacatc caattaagat tatattttga 31200 ctcttctgga aatctattaa ctgaggaatc agacttaaaa attccactta aaaataaatc 31260 ttctacagcg accagtgaaa ctgtagccag cagcaaagcc tttatgccaa gtactacagc 31320 ttatcccttc aacaccacta ctagggatag tgaaaactac attcatggaa tatgttacta 31380 catgactagt tatgatagaa gtctatttcc cttgaacatt tctataatgc taaacagccg 31440 tatgatttct tccaatgttg cctatgccat acaatttgaa tggaatctaa atgcaagtga 31500 atctccagaa agcaacatag ctacgctgac cacatccccc tttttctttt cttacattac 31560 agaagacgac aactaaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc 31620 agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca 31680 gatcaccgta ccttaatcaa actcacagaa ccctagtatt caacctgcca cctccctccc 31740 aacacacaga gtacacagtc ctttctcccc ggctggcctt aaaaagcatc atatcatggg 31800 taacagacat attcttaggt gttatattcc acacggtttc ctgtcgagcc aaacgctcat 31860 cagtgatatt aataaactcc ccgggcagct cacttaagtt catgtcgctg tccagctgct 31920 gagccacagg ctgctgtcca acttgcggtt gcttaacggg cggcgaagga gaagtccacg 31980 cctacatggg ggtagagtca taatcgtgca tcaggatagg gcggtggtgc tgcagcagcg 32040 cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga atacaacatg gcagtggtct 32100 cctcagcgat gattcgcacc gcccgcagca taaggcgcct tgtcctccgg gcacagcagc 32160 gcaccctgat ctcacttaaa tcagcacagt aactgcagca cagcaccaca atattgttca 32220 aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc ggggaccaca gaacccacgt 32280 ggccatcata ccacaagcgc aggtagatta agtggcgacc cctcataaac acgctggaca 32340 taaacattac ctcttttggc atgttgtaat tcaccacctc ccggtaccat ataaacctct 32400 gattaaacat ggcgccatcc accaccatcc taaaccagct ggccaaaacc tgcccgccgg 32460 ctatacactg cagggaaccg ggactggaac aatgacagtg gagagcccag gactcgtaac 32520 catggatcat catgctcgtc atgatatcaa tgttggcaca acacaggcac acgtgcatac 32580 acttcctcag gattacaagc tcctcccgcg ttagaaccat atcccaggga acaacccatt 32640 cctgaatcag cgtaaatccc acactgcagg gaagacctcg cacgtaactc acgttgtgca 32700 ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc cagtatggta gcgcgggttt 32760 ctgtctcaaa aggaggtaga cgatccctac tgtacggagt gcgccgagac aaccgagatc 32820 gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt agtcatattt cctgaagcaa 32880 aaccaggtgc gggcgtgaca aacagatctg cgtctccggt ctcgccgctt agatcgctct 32940 gtgtagtagt tgtagtatat ccactctctc aaagcatcca ggcgccccct ggcttcgggt 33000 tctatgtaaa ctccttcatg cgccgctgcc ctgataacat ccaccaccgc agaataagcc 33060 acacccagcc aacctacaca ttcgttctgc gagtcacaca cgggaggagc gggaagagct 33120 ggaagaacca tgtttttttt tttattccaa aagattatcc aaaacctcaa aatgaagatc 33180 tattaagtga acgcgctccc ctccggtggc gtggtcaaac tctacagcca aagaacagat 33240 aatggcattt gtaagatgtt gcacaatggc ttccaaaagg caaacggccc tcacgtccaa 33300 gtggacgtaa aggctaaacc cttcagggtg aatctcctct ataaacattc cagcaccttc 33360 aaccatgccc aaataattct catctcgcca ccttctcaat atatctctaa gcaaatcccg 33420 aatattaagt ccggccattg taaaaatctg ctccagagcg ccctccacct tcagcctcaa 33480 gcagcgaatc atgattgcaa aaattcaggt tcctcacaga cctgtataag attcaaaagc 33540 ggaacattaa caaaaatacc gcgatcccgt aggtcccttc gcagggccag ctgaacataa 33600 tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc caggaaccat gacaaaagaa 33660 cccacactga ttatgacacg catactcgga gctatgctaa ccagcgtagc cccgatgtaa 33720 gcttgttgca tgggcggcga tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc 33780 tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca gataaaggca ggtaagctcc 33840 ggaaccacca cagaaaaaga caccattttt ctctcaaaca tgtctgcggg tttctgcata 33900 aacacaaaat aaaataacaa aaaaacattt aaacattaga agcctgtctt acaacaggaa 33960 aaacaaccct tataagcata agacggacta cggccatgcc ggcgtgaccg taaaaaaact 34020 ggtcaccgtg attaaaaagc accaccgaca gctcctcggt catgtccgga gtcataatgt 34080 aagactcggt aaacacatca ggttgattca catcggtcag tgctaaaaag cgaccgaaat 34140 agcccggggg aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta 34200 taacaaaatt aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca 34260 aaatagcacc ctcccgctcc agaacaacat acagcgcttc cacagcggca gccataacag 34320 tcagccttac cagtaaaaaa gaaaacctat taaaaaaaca ccactcgaca cggcaccagc 34380 tcaatcagtc acagtgtaaa aaagggccaa gtgcagagcg agtatatata ggactaaaaa 34440 atgacgtaac ggttaaagtc cacaaaaaac acccagaaaa ccgcacgcga acctacgccc 34500 agaaacgaaa gccaaaaaac ccacaacttc ctcaaatcgt cacttccgtt ttcccacgtt 34560 acgtcacttc ccattttaag aaaactacaa ttcccaacac atacaagtta ctccgcccta 34620 aaacctacgt cacccgcccc gttcccacgc cccgcgccac gtcacaaact ccaccccctc 34680 attatcatat tggcttcaat ccaaaataag gtatattatt gatgatg 34727 <210> 26 <211> 37675 <212> DNA <213> Artificial Sequence <220> <223> plasmid #3 vector <400> 26 ttaattaaca tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 60 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 120 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 180 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 240 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 300 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 360 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 420 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 480 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 540 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 600 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 660 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 720 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 780 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 840 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 900 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 960 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1020 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1080 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1140 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1200 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1260 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1320 ttgttgccat tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca 1380 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 1440 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 1500 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 1560 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 1620 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 1680 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1740 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1800 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1860 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1920 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1980 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2040 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2100 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2160 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 2220 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2280 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2340 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2400 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2460 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 2520 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2580 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 2640 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 2700 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 2760 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 2820 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2880 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 2940 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 3000 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 3060 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 3120 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 3180 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 3240 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta 3300 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 3360 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 3420 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 3480 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 3540 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 3600 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 3660 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 3720 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3780 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3840 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 3900 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 3960 gacaccggga ccgatccagc ctccgatttg ccaccatgtt tgtgttcctg gtgctgctgc 4020 cactggtgtc cagccagtgt gtgaacctga ccaccaggac ccaacttcct cctgcctaca 4080 ccaactcctt caccagggga gtctactacc ctgacaaggt gttcaggtcc tctgtgctgc 4140 acagcaccca ggacctgttc ctgccattct tcagcaatgt gacctggttc catgccatcc 4200 atgtgtctgg caccaatggc accaagaggt ttgacaaccc tgtgctgcca ttcaatgatg 4260 gagtctactt tgccagcaca gagaagagca acatcatcag gggctggatt tttggcacca 4320 ccctggacag caagacccag tccctgctga ttgtgaacaa tgccaccaat gtggtgatta 4380 aggtgtgtga gttccagttc tgtaatgacc cattcctggg agtctactac cacaagaaca 4440 acaagtcctg gatggagtct gagttcaggg tctactcctc tgccaacaac tgtacctttg 4500 aatatgtgag ccaaccattc ctgatggact tggagggcaa gcagggcaac ttcaagaacc 4560 tgagggagtt tgtgttcaag aacattgatg gctacttcaa gatttacagc aaacacacac 4620 caatcaacct ggtgagggac ctgccacagg gcttctctgc cttggaacca ctggtggacc 4680 tgccaattgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcctacc 4740 tgacacctgg agactcctcc tctggctgga cagcaggagc agcagcctac tatgtgggct 4800 acctccaacc aaggaccttc ctgctgaaat acaatgagaa tggcaccatc acagatgctg 4860 tggactgtgc cctggaccca ctgtctgaga ccaagtgtac cctgaaatcc ttcacagtgg 4920 agaagggcat ctaccagacc agcaacttca gggtccaacc aacagagagc attgtgaggt 4980 ttccaaacat caccaacctg tgtccatttg gagaggtgtt caatgccacc aggtttgcct 5040 ctgtctatgc ctggaacagg aagaggatta gcaactgtgt ggctgactac tctgtgctct 5100 acaactctgc ctccttcagc accttcaagt gttatggagt gagcccaacc aaactgaatg 5160 acctgtgttt caccaatgtc tatgctgact cctttgtgat taggggagat gaggtgagac 5220 agattgcccc tggacaaaca ggcaagattg ctgactacaa ctacaaactg cctgatgact 5280 tcacaggctg tgtgattgcc tggaacagca acaacctgga cagcaaggtg ggaggcaact 5340 acaactacct ctacagactg ttcaggaaga gcaacctgaa accatttgag agggacatca 5400 gcacagagat ttaccaggct ggcagcacac catgtaatgg agtggagggc ttcaactgtt 5460 actttccact ccaatcctat ggcttccaac caaccaatgg agtgggctac caaccataca 5520 gggtggtggt gctgtccttt gaactgctcc atgcccctgc cacagtgtgt ggaccaaaga 5580 agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa cttcaatgga ctgacaggca 5640 caggagtgct gacagagagc aacaagaagt tcctgccatt ccaacagttt ggcagggaca 5700 ttgctgacac cacagatgct gtgagggacc cacagacctt ggagattctg gacatcacac 5760 catgttcctt tggaggagtg tctgtgatta cacctggcac caacaccagc aaccaggtgg 5820 ctgtgctcta ccaggatgtg aactgtactg aggtgcctgt ggctatccat gctgaccaac 5880 ttacaccaac ctggagggtc tacagcacag gcagcaatgt gttccagacc agggctggct 5940 gtctgattgg agcagagcat gtgaacaact cctatgagtg tgacatccca attggagcag 6000 gcatctgtgc ctcctaccag acccagaccg gtggcggtgg gtcgaggtct gtggcaagcc 6060 agagcatcat tgcctacaca atgagtctgg gagcagagaa ctctgtggct tacagcaaca 6120 acagcattgc catcccaacc aacttcacca tctctgtgac cacagagatt ctgcctgtga 6180 gtatgaccaa gacctctgtg gactgtacaa tgtatatctg tggagacagc acagagtgta 6240 gcaacctgct gctccaatat ggctccttct gtacccaact taacagggct ctgacaggca 6300 ttgctgtgga acaggacaag aacacccagg aggtgtttgc ccaggtgaag cagatttaca 6360 agacacctcc aatcaaggac tttggaggct tcaacttcag ccagattctg cctgacccaa 6420 gcaagccaag caagaggtcc ttcattgagg acctgctgtt caacaaggtg accctggctg 6480 atgctggctt catcaagcaa tatggagact gtctgggaga cattgctgcc agggacctga 6540 tttgtgccca gaagttcaat ggactgacag tgctgcctcc actgctgaca gatgagatga 6600 ttgcccaata cacctctgcc ctgctggctg gcaccatcac ctctggctgg acctttggag 6660 caggagcagc cctccaaatc ccatttgcta tgcagatggc ttacaggttc aatggcattg 6720 gagtgaccca gaatgtgctc tatgagaacc agaaactgat tgccaaccag ttcaactctg 6780 ccattggcaa gattcaggac tccctgtcca gcacagcctc tgccctgggc aaactccaag 6840 atgtggtgaa ccagaatgcc caggctctga acaccctggt gaagcaactt tccagcaact 6900 ttggagccat ctcctctgtg ctgaatgaca tcctgagcag actggacaag gtggaggctg 6960 aggtccagat tgacagactg attacaggca gactccaatc cctccaaacc tatgtgaccc 7020 aacaacttat cagggctgct gagattaggg catctgccaa cctggctgcc accaagatga 7080 gtgagtgtgt gctgggacaa agcaagaggg tggacttctg tggcaagggc taccacctga 7140 tgagttttcc acagtctgcc cctcatggag tggtgttcct gcatgtgacc tatgtgcctg 7200 cccaggagaa gaacttcacc acagcccctg ccatctgcca tgatggcaag gctcactttc 7260 caagggaggg agtgtttgtg agcaatggca cccactggtt tgtgacccag aggaacttct 7320 atgaaccaca gattatcacc acagacaaca cctttgtgtc tggcaactgt gatgtggtga 7380 ttggcattgt gaacaacaca gtctatgacc cactccaacc tgaactggac tccttcaagg 7440 aggaactgga caaatacttc aagaaccaca ccagccctga tgtggacctg ggagacatct 7500 ctggcatcaa tgcctctgtg gtgaacatcc agaaggagat tgacagactg aatgaggtgg 7560 ctaagaacct gaatgagtcc ctgattgacc tccaagaact gggcaaatat gaacaataca 7620 tcaagtggcc atgaaaattg atcataatca gccataccac atttgtagag gttttacttg 7680 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 7740 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 7800 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 7860 tatcttaacg cggatctggg cgtggttaag ggtgggaaag aatatataag gtgggggtct 7920 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga 7980 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca 8040 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt 8100 gacctacgag accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc 8160 cgctgcagcc accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag 8220 cagtgcagct tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt 8280 ggattctttg acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca 8340 ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc 8400 agactctgtt tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg 8460 cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag 8520 gacgtggtaa aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg 8580 gaggtagcac cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta 8640 gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg 8700 caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga 8760 tatgagatgc atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg 8820 gggattcatg ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc 8880 atgtagctta gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt 8940 ttccatgcat tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat 9000 atttctggga tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt 9060 tacaaagcgc gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc 9120 gtagttaccc tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc 9180 tacctgcggg gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag 9240 caggttcctg agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac 9300 cggctgcaac tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac 9360 ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc 9420 gccgcccagc gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc 9480 cgccgtaggc atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt 9540 cacctgctct acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt 9600 tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg 9660 cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg 9720 ctggccaggg tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc 9780 tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg 9840 cccttggcgc gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg 9900 agggcgtaga gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag 9960 gccccgcaga cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa 10020 accaggtttc ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt 10080 ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata cagacttgag aggcctgtcc 10140 tcgagcggtg ttccgcggtc ctcctcgtat agaaactcgg accactctga gacaaaggct 10200 cgcgtccagg ccagcacgaa ggaggctaag tgggaggggt agcggtcgtt gtccactagg 10260 gggtccactc gctccagggt gtgaagacac atgtcgccct cttcggcatc aaggaaggtg 10320 attggtttgt aggtgtaggc cacgtgaccg ggtgttcctg aaggggggct ataaaagggg 10380 gtgggggcgc gttcgtcctc actctcttcc gcatcgctgt ctgcgagggc cagctgttgg 10440 ggtgagtact ccctctgaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa 10500 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcatcc 10560 atctggtcag aaaagacaat ctttttgttg tcaagcttgg tggcaaacga cccgtagagg 10620 gcgttggaca gcaacttggc gatggagcgc agggtttggt ttttgtcgcg atcggcgcgc 10680 tccttggccg cgatgtttag ctgcacgtat tcgcgcgcaa cgcaccgcca ttcgggaaag 10740 acggtggtgc gctcgtcggg caccaggtgc acgcgccaac cgcggttgtg cagggtgaca 10800 aggtcaacgc tggtggctac ctctccgcgt aggcgctcgt tggtccagca gaggcggccg 10860 cccttgcgcg agcagaatgg cggtaggggg tctagctgcg tctcgtccgg ggggtctgcg 10920 tccacggtaa agaccccggg cagcaggcgc gcgtcgaagt agtctatctt gcatccttgc 10980 aagtctagcg cctgctgcca tgcgcgggcg gcaagcgcgc gctcgtatgg gttgagtggg 11040 ggaccccatg gcatggggtg ggtgagcgcg gaggcgtaca tgccgcaaat gtcgtaaacg 11100 tagaggggct ctctgagtat tccaagatat gtagggtagc atcttccacc gcggatgctg 11160 gcgcgcacgt aatcgtatag ttcgtgcgag ggagcgagga ggtcgggacc gaggttgcta 11220 cgggcgggct gctctgctcg gaagactatc tgcctgaaga tggcatgtga gttggatgat 11280 atggttggac gctggaagac gttgaagctg gcgtctgtga gacctaccgc gtcacgcacg 11340 aaggaggcgt aggagtcgcg cagcttgttg accagctcgg cggtgacctg cacgtctagg 11400 gcgcagtagt ccagggtttc cttgatgatg tcatacttat cctgtccctt ttttttccac 11460 agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt actcttggat cggaaacccg 11520 tcggcctccg aacggtaaga gcctagcatg tagaactggt tgacggcctg gtaggcgcag 11580 catccctttt ctacgggtag cgcgtatgcc tgcgcggcct tccggagcga ggtgtgggtg 11640 agcgcaaagg tgtccctgac catgactttg aggtactggt atttgaagtc agtgtcgtcg 11700 catccgccct gctcccagag caaaaagtcc gtgcgctttt tggaacgcgg atttggcagg 11760 gcgaaggtga catcgttgaa gagtatcttt cccgcgcgag gcataaagtt gcgtgtgatg 11820 cggaagggtc ccggcacctc ggaacggttg ttaattacct gggcggcgag cacgatctcg 11880 tcaaagccgt tgatgttgtg gcccacaatg taaagttcca agaagcgcgg gatgcccttg 11940 atggaaggca attttttaag ttcctcgtag gtgagctctt caggggagct gagcccgtgc 12000 tctgaaaggg cccagtctgc aagatgaggg ttggaagcga cgaatgagct ccacaggtca 12060 cgggccatta gcatttgcag gtggtcgcga aaggtcctaa actggcgacc tatggccatt 12120 ttttctgggg tgatgcagta gaaggtaagc gggtcttgtt cccagcggtc ccatccaagg 12180 ttcgcggcta ggtctcgcgc ggcagtcact agaggctcat ctccgccgaa cttcatgacc 12240 agcatgaagg gcacgagctg cttcccaaag gcccccatcc aagtataggt ctctacatcg 12300 taggtgacaa agagacgctc ggtgcgagga tgcgagccga tcgggaagaa ctggatctcc 12360 cgccaccaat tggaggagtg gctattgatg tggtgaaagt agaagtccct gcgacgggcc 12420 gaacactcgt gctggctttt gtaaaaacgt gcgcagtact ggcagcggtg cacgggctgt 12480 acatcctgca cgaggttgac ctgacgaccg cgcacaagga agcagagtgg gaatttgagc 12540 ccctcgcctg gcgggtttgg ctggtggtct tctacttcgg ctgcttgtcc ttgaccgtct 12600 ggctgctcga ggggagttac ggtggatcgg accaccacgc cgcgcgagcc caaagtccag 12660 atgtccgcgc gcggcggtcg gagcttgatg acaacatcgc gcagatggga gctgtccatg 12720 gtctggagct cccgcggcgt caggtcaggc gggagctcct gcaggtttac ctcgcataga 12780 cgggtcaggg cgcgggctag atccaggtga tacctaattt ccaggggctg gttggtggcg 12840 gcgtcgatgg cttgcaagag gccgcatccc cgcggcgcga ctacggtacc gcgcggcggg 12900 cggtgggccg cgggggtgtc cttggatgat gcatctaaaa gcggtgacgc gggcgagccc 12960 ccggaggtag ggggggctcc ggacccgccg ggagaggggg caggggcacg tcggcgccgc 13020 gcgcgggcag gagctggtgc tgcgcgcgta ggttgctggc gaacgcgacg acgcggcggt 13080 tgatctcctg aatctggcgc ctctgcgtga agacgacggg cccggtgagc ttgaacctga 13140 aagagagttc gacagaatca atttcggtgt cgttgacggc ggcctggcgc aaaatctcct 13200 gcacgtctcc tgagttgtct tgataggcga tctcggccat gaactgctcg atctcttcct 13260 cctggagatc tccgcgtccg gctcgctcca cggtggcggc gaggtcgttg gaaatgcggg 13320 ccatgagctg cgagaaggcg ttgaggcctc cctcgttcca gacgcggctg tagaccacgc 13380 ccccttcggc atcgcgggcg cgcatgacca cctgcgcgag attgagctcc acgtgccggg 13440 cgaagacggc gtagtttcgc aggcgctgaa agaggtagtt gagggtggtg gcggtgtgtt 13500 ctgccacgaa gaagtacata acccagcgtc gcaacgtgga ttcgttgata tcccccaagg 13560 cctcaaggcg ctccatggcc tcgtagaagt ccacggcgaa gttgaaaaac tgggagttgc 13620 gcgccgacac ggttaactcc tcctccagaa gacggatgag ctcggcgaca gtgtcgcgca 13680 cctcgcgctc aaaggctaca ggggcctctt cttcttcttc aatctcctct tccataaggg 13740 cctccccttc ttcttcttct ggcggcggtg ggggaggggg gacacggcgg cgacgacggc 13800 gcaccgggag gcggtcgaca aagcgctcga tcatctcccc gcggcgacgg cgcatggtct 13860 cggtgacggc gcggccgttc tcgcgggggc gcagttggaa gacgccgccc gtcatgtccc 13920 ggttatgggt tggcgggggg ctgccatgcg gcagggatac ggcgctaacg atgcatctca 13980 acaattgttg tgtaggtact ccgccgccga gggacctgag cgagtccgca tcgaccggat 14040 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg 14100 tggcgggcgg cagcgggcgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt 14160 aattaaagta ggcggtcttg agacggcgga tggtcgacag aagcaccatg tccttgggtc 14220 cggcctgctg aatgcgcagg cggtcggcca tgccccaggc ttcgttttga catcggcgca 14280 ggtctttgta gtagtcttgc atgagccttt ctaccggcac ttcttcttct ccttcctctt 14340 gtcctgcatc tcttgcatct atcgctgcgg cggcggcgga gtttggccgt aggtggcgcc 14400 ctcttcctcc catgcgtgtg accccgaagc ccctcatcgg ctgaagcagg gctaggtcgg 14460 cgacaacgcg ctcggctaat atggcctgct gcacctgcgt gagggtagac tggaagtcat 14520 ccatgtccac aaagcggtgg tatgcgcccg tgttgatggt gtaagtgcag ttggccataa 14580 cggaccagtt aacggtctgg tgacccggct gcgagagctc ggtgtacctg agacgcgagt 14640 aagccctcga gtcaaatacg tagtcgttgc aagtccgcac caggtactgg tatcccacca 14700 aaaagtgcgg cggcggctgg cggtagaggg gccagcgtag ggtggccggg gctccggggg 14760 cgagatcttc caacataagg cgatgatatc cgtagatgta cctggacatc caggtgatgc 14820 cggcggcggt ggtggaggcg cgcggaaagt cgcggacgcg gttccagatg ttgcgcagcg 14880 gcaaaaagtg ctccatggtc gggacgctct ggccggtcag gcgcgcgcaa tcgttgacgc 14940 tctagcgtgc aaaaggagag cctgtaagcg ggcactcttc cgtggtctgg tggataaatt 15000 cgcaagggta tcatggcgga cgaccggggt tcgagccccg tatccggccg tccgccgtga 15060 tccatgcggt taccgcccgc gtgtcgaacc caggtgtgcg acgtcagaca acgggggagt 15120 gctccttttg gcttccttcc aggcgcggcg gctgctgcgc tagctttttt ggccactggc 15180 cgcgcgcagc gtaagcggtt aggctggaaa gcgaaagcat taagtggctc gctccctgta 15240 gccggagggt tattttccaa gggttgagtc gcgggacccc cggttcgagt ctcggaccgg 15300 ccggactgcg gcgaacgggg gtttgcctcc ccgtcatgca agaccccgct tgcaaattcc 15360 tccggaaaca gggacgagcc ccttttttgc ttttcccaga tgcatccggt gctgcggcag 15420 atgcgccccc ctcctcagca gcggcaagag caagagcagc ggcagacatg cagggcaccc 15480 tcccctcctc ctaccgcgtc aggaggggcg acatccgcgg ttgacgcggc agcagatggt 15540 gattacgaac ccccgcggcg ccgggcccgg cactacctgg acttggagga gggcgagggc 15600 ctggcgcggc taggagcgcc ctctcctgag cggcacccaa gggtgcagct gaagcgtgat 15660 acgcgtgagg cgtacgtgcc gcggcagaac ctgtttcgcg accgcgaggg agaggagccc 15720 gaggagatgc gggatcgaaa gttccacgca gggcgcgagc tgcggcatgg cctgaatcgc 15780 gagcggttgc tgcgcgagga ggactttgag cccgacgcgc gaaccgggat tagtcccgcg 15840 cgcgcacacg tggcggccgc cgacctggta accgcatacg agcagacggt gaaccaggag 15900 attaactttc aaaaaagctt taacaaccac gtgcgtacgc ttgtggcgcg cgaggaggtg 15960 gctataggac tgatgcatct gtgggacttt gtaagcgcgc tggagcaaaa cccaaatagc 16020 aagccgctca tggcgcagct gttccttata gtgcagcaca gcagggacaa cgaggcattc 16080 agggatgcgc tgctaaacat agtagagccc gagggccgct ggctgctcga tttgataaac 16140 atcctgcaga gcatagtggt gcaggagcgc agcttgagcc tggctgacaa ggtggccgcc 16200 atcaactatt ccatgcttag cctgggcaag ttttacgccc gcaagatata ccatacccct 16260 tacgttccca tagacaagga ggtaaagatc gaggggttct acatgcgcat ggcgctgaag 16320 gtgcttacct tgagcgacga cctgggcgtt tatcgcaacg agcgcatcca caaggccgtg 16380 agcgtgagcc ggcggcgcga gctcagcgac cgcgagctga tgcacagcct gcaaagggcc 16440 ctggctggca cgggcagcgg cgatagagag gccgagtcct actttgacgc gggcgctgac 16500 ctgcgctggg ccccaagccg acgcgccctg gaggcagctg gggccggacc tgggctggcg 16560 gtggcacccg cgcgcgctgg caacgtcggc ggcgtggagg aatatgacga ggacgatgag 16620 tacgagccag aggacggcga gtactaagcg gtgatgtttc tgatcagatg atgcaagacg 16680 caacggaccc ggcggtgcgg gcggcgctgc agagccagcc gtccggcctt aactccacgg 16740 acgactggcg ccaggtcatg gaccgcatca tgtcgctgac tgcgcgcaat cctgacgcgt 16800 tccggcagca gccgcaggcc aaccggctct ccgcaattct ggaagcggtg gtcccggcgc 16860 gcgcaaaccc cacgcacgag aaggtgctgg cgatcgtaaa cgcgctggcc gaaaacaggg 16920 ccatccggcc cgacgaggcc ggcctggtct acgacgcgct gcttcagcgc gtggctcgtt 16980 acaacagcgg caacgtgcag accaacctgg accggctggt gggggatgtg cgcgaggccg 17040 tggcgcagcg tgagcgcgcg cagcagcagg gcaacctggg ctccatggtt gcactaaacg 17100 ccttcctgag tacacagccc gccaacgtgc cgcggggaca ggaggactac accaactttg 17160 tgagcgcact gcggctaatg gtgactgaga caccgcaaag tgaggtgtac cagtctgggc 17220 cagactattt tttccagacc agtagacaag gcctgcagac cgtaaacctg agccaggctt 17280 tcaaaaactt gcaggggctg tggggggtgc gggctcccac aggcgaccgc gcgaccgtgt 17340 ctagcttgct gacgcccaac tcgcgcctgt tgctgctgct aatagcgccc ttcacggaca 17400 gtggcagcgt gtcccgggac acatacctag gtcacttgct gacactgtac cgcgaggcca 17460 taggtcaggc gcatgtggac gagcatactt tccaggagat tacaagtgtc agccgcgcgc 17520 tggggcagga ggacacgggc agcctggagg caaccctaaa ctacctgctg accaaccggc 17580 ggcagaagat cccctcgttg cacagtttaa acagcgagga ggagcgcatt ttgcgctacg 17640 tgcagcagag cgtgagcctt aacctgatgc gcgacggggt aacgcccagc gtggcgctgg 17700 acatgaccgc gcgcaacatg gaaccgggca tgtatgcctc aaaccggccg tttatcaacc 17760 gcctaatgga ctacttgcat cgcgcggccg ccgtgaaccc cgagtatttc accaatgcca 17820 tcttgaaccc gcactggcta ccgccccctg gtttctacac cgggggattc gaggtgcccg 17880 agggtaacga tggattcctc tgggacgaca tagacgacag cgtgttttcc ccgcaaccgc 17940 agaccctgct agagttgcaa cagcgcgagc aggcagaggc ggcgctgcga aaggaaagct 18000 tccgcaggcc aagcagcttg tccgatctag gcgctgcggc cccgcggtca gatgctagta 18060 gcccatttcc aagcttgata gggtctctta ccagcactcg caccacccgc ccgcgcctgc 18120 tgggcgagga ggagtaccta aacaactcgc tgctgcagcc gcagcgcgaa aaaaacctgc 18180 ctccggcatt tcccaacaac gggatagaga gcctagtgga caagatgagt agatggaaga 18240 cgtacgcgca ggagcacagg gacgtgccag gcccgcgccc gcccacccgt cgtcaaaggc 18300 acgaccgtca gcggggtctg gtgtgggagg acgatgactc ggcagacgac agcagcgtcc 18360 tggatttggg agggagtggc aacccgtttg cgcaccttcg ccccaggctg gggagaatgt 18420 tttaaaaaaa aaaaagcatg atgcaaaata aaaaactcac caaggccatg gcaccgagcg 18480 ttggttttct tgtattcccc ttagtatgcg gcgcgcggcg atgtatgagg aaggtcctcc 18540 tccctcctac gagagtgtgg tgagcgcggc gccagtggcg gcggcgctgg gttctccctt 18600 cgatgctccc ctggacccgc cgtttgtgcc tccgcggtac ctgcggccta ccggggggag 18660 aaacagcatc cgttactctg agttggcacc cctattcgac accacccgtg tgtacctggt 18720 ggacaacaag tcaacggatg tggcatccct gaactaccag aacgaccaca gcaactttct 18780 gaccacggtc attcaaaaca atgactacag cccgggggag gcaagcacac agaccatcaa 18840 tcttgacgac cggtcgcact ggggcggcga cctgaaaacc atcctgcata ccaacatgcc 18900 aaatgtgaac gagttcatgt ttaccaataa gtttaaggcg cgggtgatgg tgtcgcgctt 18960 gcctactaag gacaatcagg tggagctgaa atacgagtgg gtggagttca cgctgcccga 19020 gggcaactac tccgagacca tgaccataga ccttatgaac aacgcgatcg tggagcacta 19080 cttgaaagtg ggcagacaga acggggttct ggaaagcgac atcggggtaa agtttgacac 19140 ccgcaacttc agactggggt ttgaccccgt cactggtctt gtcatgcctg gggtatatac 19200 aaacgaagcc ttccatccag acatcatttt gctgccagga tgcggggtgg acttcaccca 19260 cagccgcctg agcaacttgt tgggcatccg caagcggcaa cccttccagg agggctttag 19320 gatcacctac gatgatctgg agggtggtaa cattcccgca ctgttggatg tggacgccta 19380 ccaggcgagc ttgaaagatg acaccgaaca gggcgggggt ggcgcaggcg gcagcaacag 19440 cagtggcagc ggcgcggaag agaactccaa cgcggcagcc gcggcaatgc agccggtgga 19500 ggacatgaac gatcatgcca ttcgcggcga cacctttgcc acacgggctg aggagaagcg 19560 cgctgaggcc gaagcagcgg ccgaagctgc cgcccccgct gcgcaacccg aggtcgagaa 19620 gcctcagaag aaaccggtga tcaaacccct gacagaggac agcaagaaac gcagttacaa 19680 cctaataagc aatgacagca ccttcaccca gtaccgcagc tggtaccttg catacaacta 19740 cggcgaccct cagaccggaa tccgctcatg gaccctgctt tgcactcctg acgtaacctg 19800 cggctcggag caggtctact ggtcgttgcc agacatgatg caagaccccg tgaccttccg 19860 ctccacgcgc cagatcagca actttccggt ggtgggcgcc gagctgttgc ccgtgcactc 19920 caagagcttc tacaacgacc aggccgtcta ctcccaactc atccgccagt ttacctctct 19980 gacccacgtg ttcaatcgct ttcccgagaa ccagattttg gcgcgcccgc cagcccccac 20040 catcaccacc gtcagtgaaa acgttcctgc tctcacagat cacgggacgc taccgctgcg 20100 caacagcatc ggaggagtcc agcgagtgac cattactgac gccagacgcc gcacctgccc 20160 ctacgtttac aaggccctgg gcatagtctc gccgcgcgtc ctatcgagcc gcactttttg 20220 agcaagcatg tccatcctta tatcgcccag caataacaca ggctggggcc tgcgcttccc 20280 aagcaagatg tttggcgggg ccaagaagcg ctccgaccaa cacccagtgc gcgtgcgcgg 20340 gcactaccgc gcgccctggg gcgcgcacaa acgcggccgc actgggcgca ccaccgtcga 20400 tgacgccatc gacgcggtgg tggaggaggc gcgcaactac acgcccacgc cgccaccagt 20460 gtccacagtg gacgcggcca ttcagaccgt ggtgcgcgga gcccggcgct atgctaaaat 20520 gaagagacgg cggaggcgcg tagcacgtcg ccaccgccgc cgacccggca ctgccgccca 20580 acgcgcggcg gcggccctgc ttaaccgcgc acgtcgcacc ggccgacggg cggccatgcg 20640 ggccgctcga aggctggccg cgggtattgt cactgtgccc cccaggtcca ggcgacgagc 20700 ggccgccgca gcagccgcgg ccattagtgc tatgactcag ggtcgcaggg gcaacgtgta 20760 ttgggtgcgc gactcggtta gcggcctgcg cgtgcccgtg cgcacccgcc ccccgcgcaa 20820 ctagattgca agaaaaaact acttagactc gtactgttgt atgtatccag cggcggcggc 20880 gcgcaacgaa gctatgtcca agcgcaaaat caaagaagag atgctccagg tcatcgcgcc 20940 ggagatctat ggccccccga agaaggaaga gcaggattac aagccccgaa agctaaagcg 21000 ggtcaaaaag aaaaagaaag atgatgatga tgaacttgac gacgaggtgg aactgctgca 21060 cgctaccgcg cccaggcgac gggtacagtg gaaaggtcga cgcgtaaaac gtgttttgcg 21120 acccggcacc accgtagtct ttacgcccgg tgagcgctcc acccgcacct acaagcgcgt 21180 gtatgatgag gtgtacggcg acgaggacct gcttgagcag gccaacgagc gcctcgggga 21240 gtttgcctac ggaaagcggc ataaggacat gctggcgttg ccgctggacg agggcaaccc 21300 aacacctagc ctaaagcccg taacactgca gcaggtgctg cccgcgcttg caccgtccga 21360 agaaaagcgc ggcctaaagc gcgagtctgg tgacttggca cccaccgtgc agctgatggt 21420 acccaagcgc cagcgactgg aagatgtctt ggaaaaaatg accgtggaac ctgggctgga 21480 gcccgaggtc cgcgtgcggc caatcaagca ggtggcgccg ggactgggcg tgcagaccgt 21540 ggacgttcag atacccacta ccagtagcac cagtattgcc accgccacag agggcatgga 21600 gacacaaacg tccccggttg cctcagcggt ggcggatgcc gcggtgcagg cggtcgctgc 21660 ggccgcgtcc aagacctcta cggaggtgca aacggacccg tggatgtttc gcgtttcagc 21720 cccccggcgc ccgcgccgtt cgaggaagta cggcgccgcc agcgcgctac tgcccgaata 21780 tgccctacat ccttccattg cgcctacccc cggctatcgt ggctacacct accgccccag 21840 aagacgagca actacccgac gccgaaccac cactggaacc cgccgccgcc gtcgccgtcg 21900 ccagcccgtg ctggccccga tttccgtgcg cagggtggct cgcgaaggag gcaggaccct 21960 ggtgctgcca acagcgcgct accaccccag catcgtttaa aagccggtct ttgtggttct 22020 tgcagatatg gccctcacct gccgcctccg tttcccggtg ccgggattcc gaggaagaat 22080 gcaccgtagg aggggcatgg ccggccacgg cctgacgggc ggcatgcgtc gtgcgcacca 22140 ccggcggcgg cgcgcgtcgc accgtcgcat gcgcggcggt atcctgcccc tccttattcc 22200 actgatcgcc gcggcgattg gcgccgtgcc cggaattgca tccgtggcct tgcaggcgca 22260 gagacactga ttaaaaacaa gttgcatgtg gaaaaatcaa aataaaaagt ctggactctc 22320 acgctcgctt ggtcctgtaa ctattttgta gaatggaaga catcaacttt gcgtctctgg 22380 ccccgcgaca cggctcgcgc ccgttcatgg gaaactggca agatatcggc accagcaata 22440 tgagcggtgg cgccttcagc tggggctcgc tgtggagcgg cattaaaaat ttcggttcca 22500 ccgttaagaa ctatggcagc aaggcctgga acagcagcac aggccagatg ctgagggata 22560 agttgaaaga gcaaaatttc caacaaaagg tggtagatgg cctggcctct ggcattagcg 22620 gggtggtgga cctggccaac caggcagtgc aaaataagat taacagtaag cttgatcccc 22680 gccctcccgt agaggagcct ccaccggccg tggagacagt gtctccagag gggcgtggcg 22740 aaaagcgtcc gcgccccgac agggaagaaa ctctggtgac gcaaatagac gagcctccct 22800 cgtacgagga ggcactaaag caaggcctgc ccaccacccg tcccatcgcg cccatggcta 22860 ccggagtgct gggccagcac acacccgtaa cgctggacct gcctcccccc gccgacaccc 22920 agcagaaacc tgtgctgcca ggcccgaccg ccgttgttgt aacccgtcct agccgcgcgt 22980 ccctgcgccg cgccgccagc ggtccgcgat cgttgcggcc cgtagccagt ggcaactggc 23040 aaagcacact gaacagcatc gtgggtctgg gggtgcaatc cctgaagcgc cgacgatgct 23100 tctgatagct aacgtgtcgt atgtgtgtca tgtatgcgtc catgtcgccg ccagaggagc 23160 tgctgagccg ccgcgcgccc gctttccaag atggctaccc cttcgatgat gccgcagtgg 23220 tcttacatgc acatctcggg ccaggacgcc tcggagtacc tgagccccgg gctggtgcag 23280 tttgcccgcg ccaccgagac gtacttcagc ctgaataaca agtttagaaa ccccacggtg 23340 gcgcctacgc acgacgtgac cacagaccgg tcccagcgtt tgacgctgcg gttcatccct 23400 gtggaccgtg aggatactgc gtactcgtac aaggcgcggt tcaccctagc tgtgggtgat 23460 aaccgtgtgc tggacatggc ttccacgtac tttgacatcc gcggcgtgct ggacaggggc 23520 cctactttta agccctactc tggcactgcc tacaacgccc tggctcccaa gggtgcccca 23580 aatccttgcg aatgggatga agctgctact gctcttgaaa taaacctaga agaagaggac 23640 gatgacaacg aagacgaagt agacgagcaa gctgagcagc aaaaaactca cgtatttggg 23700 caggcgcctt attctggtat aaatattaca aaggagggta ttcaaatagg tgtcgaaggt 23760 caaacaccta aatatgccga taaaacattt caacctgaac ctcaaatagg agaatctcag 23820 tggtacgaaa cagaaattaa tcatgcagct gggagagtcc taaaaaagac taccccaatg 23880 aaaccatgtt acggttcata tgcaaaaccc acaaatgaaa atggagggca aggcattctt 23940 gtaaagcaac aaaatggaaa gctagaaagt caagtggaaa tgcaattttt ctcaactact 24000 gaggcagccg caggcaatgg tgataacttg actcctaaag tggtattgta cagtgaagat 24060 gtagatatag aaaccccaga cactcatatt tcttacatgc ccactattaa ggaaggtaac 24120 tcacgagaac taatgggcca acaatctatg cccaacaggc ctaattacat tgcttttagg 24180 gacaatttta ttggtctaat gtattacaac agcacgggta atatgggtgt tctggcgggc 24240 caagcatcgc agttgaatgc tgttgtagat ttgcaagaca gaaacacaga gctttcatac 24300 cagcttttgc ttgattccat tggtgataga accaggtact tttctatgtg gaatcaggct 24360 gttgacagct atgatccaga tgttagaatt attgaaaatc atggaactga agatgaactt 24420 ccaaattact gctttccact gggaggtgtg attaatacag agactcttac caaggtaaaa 24480 cctaaaacag gtcaggaaaa tggatgggaa aaagatgcta cagaattttc agataaaaat 24540 gaaataagag ttggaaataa ttttgccatg gaaatcaatc taaatgccaa cctgtggaga 24600 aatttcctgt actccaacat agcgctgtat ttgcccgaca agctaaagta cagtccttcc 24660 aacgtaaaaa tttctgataa cccaaacacc tacgactaca tgaacaagcg agtggtggct 24720 cccgggctag tggactgcta cattaacctt ggagcacgct ggtcccttga ctatatggac 24780 aacgtcaacc catttaacca ccaccgcaat gctggcctgc gctaccgctc aatgttgctg 24840 ggcaatggtc gctatgtgcc cttccacatc caggtgcctc agaagttctt tgccattaaa 24900 aacctccttc tcctgccggg ctcatacacc tacgagtgga acttcaggaa ggatgttaac 24960 atggttctgc agagctccct aggaaatgac ctaagggttg acggagccag cattaagttt 25020 gatagcattt gcctttacgc caccttcttc cccatggccc acaacaccgc ctccacgctt 25080 gaggccatgc ttagaaacga caccaacgac cagtccttta acgactatct ctccgccgcc 25140 aacatgctct accctatacc cgccaacgct accaacgtgc ccatatccat cccctcccgc 25200 aactgggcgg ctttccgcgg ctgggccttc acgcgcctta agactaagga aaccccatca 25260 ctgggctcgg gctacgaccc ttattacacc tactctggct ctatacccta cctagatgga 25320 accttttacc tcaaccacac ctttaagaag gtggccatta cctttgactc ttctgtcagc 25380 tggcctggca atgaccgcct gcttaccccc aacgagtttg aaattaagcg ctcagttgac 25440 ggggagggtt acaacgttgc ccagtgtaac atgaccaaag actggttcct ggtacaaatg 25500 ctagctaact ataacattgg ctaccagggc ttctatatcc cagagagcta caaggaccgc 25560 atgtactcct tctttagaaa cttccagccc atgagccgtc aggtggtgga tgatactaaa 25620 tacaaggact accaacaggt gggcatccta caccaacaca acaactctgg atttgttggc 25680 taccttgccc ccaccatgcg cgaaggacag gcctaccctg ctaacttccc ctatccgctt 25740 ataggcaaga ccgcagttga cagcattacc cagaaaaagt ttctttgcga tcgcaccctt 25800 tggcgcatcc cattctccag taactttatg tccatgggcg cactcacaga cctgggccaa 25860 aaccttctct acgccaactc cgcccacgcg ctagacatga cttttgaggt ggatcccatg 25920 gacgagccca cccttcttta tgttttgttt gaagtctttg acgtggtccg tgtgcaccag 25980 ccgcaccgcg gcgtcatcga aaccgtgtac ctgcgcacgc ccttctcggc cggcaacgcc 26040 acaacataaa gaagcaagca acatcaacaa cagctgccgc catgggctcc agtgagcagg 26100 aactgaaagc cattgtcaaa gatcttggtt gtgggccata ttttttgggc acctatgaca 26160 agcgctttcc aggctttgtt tctccacaca agctcgcctg cgccatagtc aatacggccg 26220 gtcgcgagac tgggggcgta cactggatgg cctttgcctg gaacccgcac tcaaaaacat 26280 gctacctctt tgagcccttt ggcttttctg accagcgact caagcaggtt taccagtttg 26340 agtacgagtc actcctgcgc cgtagcgcca ttgcttcttc ccccgaccgc tgtataacgc 26400 tggaaaagtc cacccaaagc gtacaggggc ccaactcggc cgcctgtgga ctattctgct 26460 gcatgtttct ccacgccttt gccaactggc cccaaactcc catggatcac aaccccacca 26520 tgaaccttat taccggggta cccaactcca tgctcaacag tccccaggta cagcccaccc 26580 tgcgtcgcaa ccaggaacag ctctacagct tcctggagcg ccactcgccc tacttccgca 26640 gccacagtgc gcagattagg agcgccactt ctttttgtca cttgaaaaac atgtaaaaat 26700 aatgtactag agacactttc aataaaggca aatgctttta tttgtacact ctcgggtgat 26760 tatttacccc cacccttgcc gtctgcgccg tttaaaaatc aaaggggttc tgccgcgcat 26820 cgctatgcgc cactggcagg gacacgttgc gatactggtg tttagtgctc cacttaaact 26880 caggcacaac catccgcggc agctcggtga agttttcact ccacaggctg cgcaccatca 26940 ccaacgcgtt tagcaggtcg ggcgccgata tcttgaagtc gcagttgggg cctccgccct 27000 gcgcgcgcga gttgcgatac acagggttgc agcactggaa cactatcagc gccgggtggt 27060 gcacgctggc cagcacgctc ttgtcggaga tcagatccgc gtccaggtcc tccgcgttgc 27120 tcagggcgaa cggagtcaac tttggtagct gccttcccaa aaagggcgcg tgcccaggct 27180 ttgagttgca ctcgcaccgt agtggcatca aaaggtgacc gtgcccggtc tgggcgttag 27240 gatacagcgc ctgcataaaa gccttgatct gcttaaaagc cacctgagcc tttgcgcctt 27300 cagagaagaa catgccgcaa gacttgccgg aaaactgatt ggccggacag gccgcgtcgt 27360 gcacgcagca ccttgcgtcg gtgttggaga tctgcaccac atttcggccc caccggttct 27420 tcacgatctt ggccttgcta gactgctcct tcagcgcgcg ctgcccgttt tcgctcgtca 27480 catccatttc aatcacgtgc tccttattta tcataatgct tccgtgtaga cacttaagct 27540 cgccttcgat ctcagcgcag cggtgcagcc acaacgcgca gcccgtgggc tcgtgatgct 27600 tgtaggtcac ctctgcaaac gactgcaggt acgcctgcag gaatcgcccc atcatcgtca 27660 caaaggtctt gttgctggtg aaggtcagct gcaacccgcg gtgctcctcg ttcagccagg 27720 tcttgcatac ggccgccaga gcttccactt ggtcaggcag tagtttgaag ttcgccttta 27780 gatcgttatc cacgtggtac ttgtccatca gcgcgcgcgc agcctccatg cccttctccc 27840 acgcagacac gatcggcaca ctcagcgggt tcatcaccgt aatttcactt tccgcttcgc 27900 tgggctcttc ctcttcctct tgcgtccgca taccacgcgc cactgggtcg tcttcattca 27960 gccgccgcac tgtgcgctta cctcctttgc catgcttgat tagcaccggt gggttgctga 28020 aacccaccat ttgtagcgcc acatcttctc tttcttcctc gctgtccacg attacctctg 28080 gtgatggcgg gcgctcgggc ttgggagaag ggcgcttctt tttcttcttg ggcgcaatgg 28140 ccaaatccgc cgccgaggtc gatggccgcg ggctgggtgt gcgcggcacc agcgcgtctt 28200 gtgatgagtc ttcctcgtcc tcggactcga tacgccgcct catccgcttt tttgggggcg 28260 cccggggagg cggcggcgac ggggacgggg acgacacgtc ctccatggtt gggggacgtc 28320 gcgccgcacc gcgtccgcgc tcgggggtgg tttcgcgctg ctcctcttcc cgactggcca 28380 tttccttctc ctataggcag aaaaagatca tggagtcagt cgagaagaag gacagcctaa 28440 ccgccccctc tgagttcgcc accaccgcct ccaccgatgc cgccaacgcg cctaccacct 28500 tccccgtcga ggcacccccg cttgaggagg aggaagtgat tatcgagcag gacccaggtt 28560 ttgtaagcga agacgacgag gaccgctcag taccaacaga ggataaaaag caagaccagg 28620 acaacgcaga ggcaaacgag gaacaagtcg ggcgggggga cgaaaggcat ggcgactacc 28680 tagatgtggg agacgacgtg ctgttgaagc atctgcagcg ccagtgcgcc attatctgcg 28740 acgcgttgca agagcgcagc gatgtgcccc tcgccatagc ggatgtcagc cttgcctacg 28800 aacgccacct attctcaccg cgcgtacccc ccaaacgcca agaaaacggc acatgcgagc 28860 ccaacccgcg cctcaacttc taccccgtat ttgccgtgcc agaggtgctt gccacctatc 28920 acatcttttt ccaaaactgc aagatacccc tatcctgccg tgccaaccgc agccgagcgg 28980 acaagcagct ggccttgcgg cagggcgctg tcatacctga tatcgcctcg ctcaacgaag 29040 tgccaaaaat ctttgagggt cttggacgcg acgagaagcg cgcggcaaac gctctgcaac 29100 aggaaaacag cgaaaatgaa agtcactctg gagtgttggt ggaactcgag ggtgacaacg 29160 cgcgcctagc cgtactaaaa cgcagcatcg aggtcaccca ctttgcctac ccggcactta 29220 acctaccccc caaggtcatg agcacagtca tgagtgagct gatcgtgcgc cgtgcgcagc 29280 ccctggagag ggatgcaaat ttgcaagaac aaacagagga gggcctaccc gcagttggcg 29340 acgagcagct agcgcgctgg cttcaaacgc gcgagcctgc cgacttggag gagcgacgca 29400 aactaatgat ggccgcagtg ctcgttaccg tggagcttga gtgcatgcag cggttctttg 29460 ctgacccgga gatgcagcgc aagctagagg aaacattgca ctacaccttt cgacagggct 29520 acgtacgcca ggcctgcaag atctccaacg tggagctctg caacctggtc tcctaccttg 29580 gaattttgca cgaaaaccgc cttgggcaaa acgtgcttca ttccacgctc aagggcgagg 29640 cgcgccgcga ctacgtccgc gactgcgttt acttatttct atgctacacc tggcagacgg 29700 ccatgggcgt ttggcagcag tgcttggagg agtgcaacct caaggagctg cagaaactgc 29760 taaagcaaaa cttgaaggac ctatggacgg ccttcaacga gcgctccgtg gccgcgcacc 29820 tggcggacat cattttcccc gaacgcctgc ttaaaaccct gcaacagggt ctgccagact 29880 tcaccagtca aagcatgttg cagaacttta ggaactttat cctagagcgc tcaggaatct 29940 tgcccgccac ctgctgtgca cttcctagcg actttgtgcc cattaagtac cgcgaatgcc 30000 ctccgccgct ttggggccac tgctaccttc tgcagctagc caactacctt gcctaccact 30060 ctgacataat ggaagacgtg agcggtgacg gtctactgga gtgtcactgt cgctgcaacc 30120 tatgcacccc gcaccgctcc ctggtttgca attcgcagct gcttaacgaa agtcaaatta 30180 tcggtacctt tgagctgcag ggtccctcgc ctgacgaaaa gtccgcggct ccggggttga 30240 aactcactcc ggggctgtgg acgtcggctt accttcgcaa atttgtacct gaggactacc 30300 acgcccacga gattaggttc tacgaagacc aatcccgccc gcctaatgcg gagcttaccg 30360 cctgcgtcat tacccagggc cacattcttg gccaattgca agccatcaac aaagcccgcc 30420 aagagtttct gctacgaaag ggacgggggg tttacttgga cccccagtcc ggcgaggagc 30480 tcaacccaat ccccccgccg ccgcagccct atcagcagca gccgcgggcc cttgcttccc 30540 aggatggcac ccaaaaagaa gctgcagctg ccgccgccac ccacggacga ggaggaatac 30600 tgggacagtc aggcagagga ggttttggac gaggaggagg aggacatgat ggaagactgg 30660 gagagcctag acgaggaagc ttccgaggtc gaagaggtgt cagacgaaac accgtcaccc 30720 tcggtcgcat tcccctcgcc ggcgccccag aaatcggcaa ccggttccag catggctaca 30780 acctccgctc ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac 30840 accactggaa ccagggccgg taagtccaag cagccgccgc cgttagccca agagcaacaa 30900 cagcgccaag gctaccgctc atggcgcggg cacaagaacg ccatagttgc ttgcttgcaa 30960 gactgtgggg gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc 31020 ttcccccgta acatcctgca ttactaccgt catctctaca gcccatactg caccggcggc 31080 agcggcagca acagcagcgg ccacacagaa gcaaaggcga ccggatagca agactctgac 31140 aaagcccaag aaatccacag cggcggcagc agcaggagga ggagcgctgc gtctggcgcc 31200 caacgaaccc gtatcgaccc gcgagcttag aaacaggatt tttcccactc tgtatgctat 31260 atttcaacag agcaggggcc aagaacaaga gctgaaaata aaaaacaggt ctctgcgatc 31320 cctcacccgc agctgcctgt atcacaaaag cgaagatcag cttcggcgca cgctggaaga 31380 cgcggaggct ctcttcagta aatactgcgc gctgactctt aaggactagt ttcgcgccct 31440 ttctcaaatt taagcgcgaa aactacgtca tctccagcgg ccacacccgg cgccagcacc 31500 tgttgtcagc gccattatga gcaaggaaat tcccacgccc tacatgtgga gttaccagcc 31560 acaaatggga cttgcggctg gagctgccca agactactca acccgaataa actacatgag 31620 cgcgggaccc cacatgatat cccgggtcaa cggaatacgc gcccaccgaa accgaattct 31680 cctggaacag gcggctatta ccaccacacc tcgtaataac cttaatcccc gtagttggcc 31740 cgctgccctg gtgtaccagg aaagtcccgc tcccaccact gtggtacttc ccagagacgc 31800 ccaggccgaa gttcagatga ctaactcagg ggcgcagctt gcgggcggct ttcgtcacag 31860 ggtgcggtcg cccgggcagg gtataactca cctgacaatc agagggcgag gtattcagct 31920 caacgacgag tcggtgagct cctcgcttgg tctccgtccg gacgggacat ttcagatcgg 31980 cggcgccggc cgctcttcat tcacgcctcg tcaggcaatc ctaactctgc agacctcgtc 32040 ctctgagccg cgctctggag gcattggaac tctgcaattt attgaggagt ttgtgccatc 32100 ggtctacttt aaccccttct cgggacctcc cggccactat ccggatcaat ttattcctaa 32160 ctttgacgcg gtaaaggact cggcggacgg ctacgactga atgttaagtg gagaggcaga 32220 gcaactgcgc ctgaaacacc tggtccactg tcgccgccac aagtgctttg cccgcgactc 32280 cggtgagttt tgctactttg aattgcccga ggatcatatc gagggcccgg cgcacggcgt 32340 ccggcttacc gcccagggag agcttgcccg tagcctgatt cgggagttta cccagcgccc 32400 cctgctagtt gagcgggaca ggggaccctg tgttctcact gtgatttgca actgtcctaa 32460 ccctggatta catcaagatc ctctagttaa tgtcaggtcg cctaagtcga ttaactagag 32520 tacccgggga tcttattccc tttaactaat aaaaaaaaat aataaagcat cacttactta 32580 aaatcagtta gcaaatttct gtccagttta ttcagcagca cctccttgcc ctcctcccag 32640 ctctggtatt gcagcttcct cctggctgca aactttctcc acaatctaaa tggaatgtca 32700 gtttcctcct gttcctgtcc atccgcaccc actatcttca tgttgttgca gatgaagcgc 32760 gcaagaccgt ctgaagatac cttcaacccc gtgtatccat atgacacgga aaccggtcct 32820 ccaactgtgc cttttcttac tcctcccttt gtatccccca atgggtttca agagagtccc 32880 cctggggtac tctctttgcg cctatccgaa cctctagtta cctccaatgg catgcttgcg 32940 ctcaaaatgg gcaacggcct ctctctggac gaggccggca accttacctc ccaaaatgta 33000 accactgtga gcccacctct caaaaaaacc aagtcaaaca taaacctgga aatatctgca 33060 cccctcacag ttacctcaga agccctaact gtggctgccg ccgcacctct aatggtcgcg 33120 ggcaacacac tcaccatgca atcacaggcc ccgctaaccg tgcacgactc caaacttagc 33180 attgccaccc aaggacccct cacagtgtca gaaggaaagc tagccctgca aacatcaggc 33240 cccctcacca ccaccgatag cagtaccctt actatcactg cctcaccccc tctaactact 33300 gccactggta gcttgggcat tgacttgaaa gagcccattt atacacaaaa tggaaaacta 33360 ggactaaagt acggggctcc tttgcatgta acagacgacc taaacacttt gaccgtagca 33420 actggtccag gtgtgactat taataatact tccttgcaaa ctaaagttac tggagccttg 33480 ggttttgatt cacaaggcaa tatgcaactt aatgtagcag gaggactaag gattgattct 33540 caaaacagac gccttatact tgatgttagt tatccgtttg atgctcaaaa ccaactaaat 33600 ctaagactag gacagggccc tctttttata aactcagccc acaacttgga tattaactac 33660 aacaaaggcc tttacttgtt tacagcttca aacaattcca aaaagcttga ggttaaccta 33720 agcactgcca aggggttgat gtttgacgct acagccatag ccattaatgc aggagatggg 33780 cttgaatttg gttcacctaa tgcaccaaac acaaatcccc tcaaaacaaa aattggccat 33840 ggcctagaat ttgattcaaa caaggctatg gttcctaaac taggaactgg ccttagtttt 33900 gacagcacag gtgccattac agtaggaaac aaaaataatg ataagctaac tttgtggacc 33960 ggaataaacc ctccacctaa ctgtcaaatt gtggaaaaca ctaatacaaa tgatggcaaa 34020 cttactttag tattagtaaa aaatggaggg cttgttaatg gctacgtgtc tctagttggt 34080 gtatcagaca ctgtgaacca aatgttcaca caaaagacag caaacatcca attaagatta 34140 tattttgact cttctggaaa tctattaact gaggaatcag acttaaaaat tccacttaaa 34200 aataaatctt ctacagcgac cagtgaaact gtagccagca gcaaagcctt tatgccaagt 34260 actacagctt atcccttcaa caccactact agggatagtg aaaactacat tcatggaata 34320 tgttactaca tgactagtta tgatagaagt ctatttccct tgaacatttc tataatgcta 34380 aacagccgta tgatttcttc caatgttgcc tatgccatac aatttgaatg gaatctaaat 34440 gcaagtgaat ctccagaaag caacatagct acgctgacca catccccctt tttcttttct 34500 tacattacag aagacgacaa ctaaagaatc gtttgtgtta tgtttcaacg tgtttatttt 34560 tcaattgcag aaaatttcaa gtcatttttc attcagtagt atagccccac caccacatag 34620 cttatacaga tcaccgtacc ttaatcaaac tcacagaacc ctagtattca acctgccacc 34680 tccctcccaa cacacagagt acacagtcct ttctccccgg ctggccttaa aaagcatcat 34740 atcatgggta acagacatat tcttaggtgt tatattccac acggtttcct gtcgagccaa 34800 acgctcatca gtgatattaa taaactcccc gggcagctca cttaagttca tgtcgctgtc 34860 cagctgctga gccacaggct gctgtccaac ttgcggttgc ttaacgggcg gcgaaggaga 34920 agtccacgcc tacatggggg tagagtcata atcgtgcatc aggatagggc ggtggtgctg 34980 cagcagcgcg cgaataaact gctgccgccg ccgctccgtc ctgcaggaat acaacatggc 35040 agtggtctcc tcagcgatga ttcgcaccgc ccgcagcata aggcgccttg tcctccgggc 35100 acagcagcgc accctgatct cacttaaatc agcacagtaa ctgcagcaca gcaccacaat 35160 attgttcaaa atcccacagt gcaaggcgct gtatccaaag ctcatggcgg ggaccacaga 35220 acccacgtgg ccatcatacc acaagcgcag gtagattaag tggcgacccc tcataaacac 35280 gctggacata aacattacct cttttggcat gttgtaattc accacctccc ggtaccatat 35340 aaacctctga ttaaacatgg cgccatccac caccatccta aaccagctgg ccaaaacctg 35400 cccgccggct atacactgca gggaaccggg actggaacaa tgacagtgga gagcccagga 35460 ctcgtaacca tggatcatca tgctcgtcat gatatcaatg ttggcacaac acaggcacac 35520 gtgcatacac ttcctcagga ttacaagctc ctcccgcgtt agaaccatat cccagggaac 35580 aacccattcc tgaatcagcg taaatcccac actgcaggga agacctcgca cgtaactcac 35640 gttgtgcatt gtcaaagtgt tacattcggg cagcagcgga tgatcctcca gtatggtagc 35700 gcgggtttct gtctcaaaag gaggtagacg atccctactg tacggagtgc gccgagacaa 35760 ccgagatcgt gttggtcgta gtgtcatgcc aaatggaacg ccggacgtag tcatatttcc 35820 tgaagcaaaa ccaggtgcgg gcgtgacaaa cagatctgcg tctccggtct cgccgcttag 35880 atcgctctgt gtagtagttg tagtatatcc actctctcaa agcatccagg cgccccctgg 35940 cttcgggttc tatgtaaact ccttcatgcg ccgctgccct gataacatcc accaccgcag 36000 aataagccac acccagccaa cctacacatt cgttctgcga gtcacacacg ggaggagcgg 36060 gaagagctgg aagaaccatg tttttttttt tattccaaaa gattatccaa aacctcaaaa 36120 tgaagatcta ttaagtgaac gcgctcccct ccggtggcgt ggtcaaactc tacagccaaa 36180 gaacagataa tggcatttgt aagatgttgc acaatggctt ccaaaaggca aacggccctc 36240 acgtccaagt ggacgtaaag gctaaaccct tcagggtgaa tctcctctat aaacattcca 36300 gcaccttcaa ccatgcccaa ataattctca tctcgccacc ttctcaatat atctctaagc 36360 aaatcccgaa tattaagtcc ggccattgta aaaatctgct ccagagcgcc ctccaccttc 36420 agcctcaagc agcgaatcat gattgcaaaa attcaggttc ctcacagacc tgtataagat 36480 tcaaaagcgg aacattaaca aaaataccgc gatcccgtag gtcccttcgc agggccagct 36540 gaacataatc gtgcaggtct gcacggacca gcgcggccac ttccccgcca ggaaccatga 36600 caaaagaacc cacactgatt atgacacgca tactcggagc tatgctaacc agcgtagccc 36660 cgatgtaagc ttgttgcatg ggcggcgata taaaatgcaa ggtgctgctc aaaaaatcag 36720 gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg ctcatgcaga taaaggcagg 36780 taagctccgg aaccaccaca gaaaaagaca ccatttttct ctcaaacatg tctgcgggtt 36840 tctgcataaa cacaaaataa aataacaaaa aaacatttaa acattagaag cctgtcttac 36900 aacaggaaaa acaaccctta taagcataag acggactacg gccatgccgg cgtgaccgta 36960 aaaaaactgg tcaccgtgat taaaaagcac caccgacagc tcctcggtca tgtccggagt 37020 cataatgtaa gactcggtaa acacatcagg ttgattcaca tcggtcagtg ctaaaaagcg 37080 accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta cagcccccat 37140 aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa aaccctcctg 37200 cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcca cagcggcagc 37260 cataacagtc agccttacca gtaaaaaaga aaacctatta aaaaaacacc actcgacacg 37320 gcaccagctc aatcagtcac agtgtaaaaa agggccaagt gcagagcgag tatatatagg 37380 actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac 37440 ctacgcccag aaacgaaagc caaaaaaccc acaacttcct caaatcgtca cttccgtttt 37500 cccacgttac gtcacttccc attttaagaa aactacaatt cccaacacat acaagttact 37560 ccgccctaaa acctacgtca cccgccccgt tcccacgccc cgcgccacgt cacaaactcc 37620 accccctcat tatcatattg gcttcaatcc aaaataaggt atattattga tgatg 37675 <210> 27 <211> 34736 <212> DNA <213> Artificial Sequence <220> <223> #3 vector <400> 27 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct 960 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 1020 agacaccggg accgatccag cctccgattt gccaccatgt ttgtgttcct ggtgctgctg 1080 ccactggtgt ccagccagtg tgtgaacctg accaccagga cccaacttcc tcctgcctac 1140 accaactcct tcaccagggg agtctactac cctgacaagg tgttcaggtc ctctgtgctg 1200 cacagcaccc aggacctgtt cctgccattc ttcagcaatg tgacctggtt ccatgccatc 1260 catgtgtctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc attcaatgat 1320 ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat ttttggcacc 1380 accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa tgtggtgatt 1440 aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg gagtctacta ccacaagaac 1500 aacaagtcct ggatggagtc tgagttcagg gtctactcct ctgccaacaa ctgtaccttt 1560 gaatatgtga gccaaccatt cctgatggac ttggagggca agcagggcaa cttcaagaac 1620 ctgagggagt ttgtgttcaa gaacattgat ggctacttca agatttacag caaacacaca 1680 ccaatcaacc tggtgaggga cctgccacag ggcttctctg ccttggaacc actggtggac 1740 ctgccaattg gcatcaacat caccaggttc cagaccctgc tggctctgca caggtcctac 1800 ctgacacctg gagactcctc ctctggctgg acagcaggag cagcagccta ctatgtgggc 1860 tacctccaac caaggacctt cctgctgaaa tacaatgaga atggcaccat cacagatgct 1920 gtggactgtg ccctggaccc actgtctgag accaagtgta ccctgaaatc cttcacagtg 1980 gagaagggca tctaccagac cagcaacttc agggtccaac caacagagag cattgtgagg 2040 tttccaaaca tcaccaacct gtgtccattt ggagaggtgt tcaatgccac caggtttgcc 2100 tctgtctatg cctggaacag gaagaggatt agcaactgtg tggctgacta ctctgtgctc 2160 tacaactctg cctccttcag caccttcaag tgttatggag tgagcccaac caaactgaat 2220 gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga ttaggggaga tgaggtgaga 2280 cagattgccc ctggacaaac aggcaagatt gctgactaca actacaaact gcctgatgac 2340 ttcacaggct gtgtgattgc ctggaacagc aacaacctgg acagcaaggt gggaggcaac 2400 tacaactacc tctacagact gttcaggaag agcaacctga aaccatttga gagggacatc 2460 agcacagaga tttaccaggc tggcagcaca ccatgtaatg gagtggaggg cttcaactgt 2520 tactttccac tccaatccta tggcttccaa ccaaccaatg gagtgggcta ccaaccatac 2580 agggtggtgg tgctgtcctt tgaactgctc catgcccctg ccacagtgtg tggaccaaag 2640 aagagcacca acctggtgaa gaacaagtgt gtgaacttca acttcaatgg actgacaggc 2700 acaggagtgc tgacagagag caacaagaag ttcctgccat tccaacagtt tggcagggac 2760 attgctgaca ccacagatgc tgtgagggac ccacagacct tggagattct ggacatcaca 2820 ccatgttcct ttggaggagt gtctgtgatt acacctggca ccaacaccag caaccaggtg 2880 gctgtgctct accaggatgt gaactgtact gaggtgcctg tggctatcca tgctgaccaa 2940 cttacaccaa cctggagggt ctacagcaca ggcagcaatg tgttccagac cagggctggc 3000 tgtctgattg gagcagagca tgtgaacaac tcctatgagt gtgacatccc aattggagca 3060 ggcatctgtg cctcctacca gacccagacc ggtggcggtg ggtcgaggtc tgtggcaagc 3120 cagagcatca ttgcctacac aatgagtctg ggagcagaga actctgtggc ttacagcaac 3180 aacagcattg ccatcccaac caacttcacc atctctgtga ccacagagat tctgcctgtg 3240 agtatgacca agacctctgt ggactgtaca atgtatatct gtggagacag cacagagtgt 3300 agcaacctgc tgctccaata tggctccttc tgtacccaac ttaacagggc tctgacaggc 3360 attgctgtgg aacaggacaa gaacacccag gaggtgtttg cccaggtgaa gcagatttac 3420 aagacacctc caatcaagga ctttggaggc ttcaacttca gccagattct gcctgaccca 3480 agcaagccaa gcaagaggtc cttcattgag gacctgctgt tcaacaaggt gaccctggct 3540 gatgctggct tcatcaagca atatggagac tgtctgggag acattgctgc cagggacctg 3600 atttgtgccc agaagttcaa tggactgaca gtgctgcctc cactgctgac agatgagatg 3660 attgcccaat acacctctgc cctgctggct ggcaccatca cctctggctg gacctttgga 3720 gcaggagcag ccctccaaat cccatttgct atgcagatgg cttacaggtt caatggcatt 3780 ggagtgaccc agaatgtgct ctatgagaac cagaaactga ttgccaacca gttcaactct 3840 gccattggca agattcagga ctccctgtcc agcacagcct ctgccctggg caaactccaa 3900 gatgtggtga accagaatgc ccaggctctg aacaccctgg tgaagcaact ttccagcaac 3960 tttggagcca tctcctctgt gctgaatgac atcctgagca gactggacaa ggtggaggct 4020 gaggtccaga ttgacagact gattacaggc agactccaat ccctccaaac ctatgtgacc 4080 caacaactta tcagggctgc tgagattagg gcatctgcca acctggctgc caccaagatg 4140 agtgagtgtg tgctgggaca aagcaagagg gtggacttct gtggcaaggg ctaccacctg 4200 atgagttttc cacagtctgc ccctcatgga gtggtgttcc tgcatgtgac ctatgtgcct 4260 gcccaggaga agaacttcac cacagcccct gccatctgcc atgatggcaa ggctcacttt 4320 ccaagggagg gagtgtttgt gagcaatggc acccactggt ttgtgaccca gaggaacttc 4380 tatgaaccac agattatcac cacagacaac acctttgtgt ctggcaactg tgatgtggtg 4440 attggcattg tgaacaacac agtctatgac ccactccaac ctgaactgga ctccttcaag 4500 gaggaactgg acaaatactt caagaaccac accagccctg atgtggacct gggagacatc 4560 tctggcatca atgcctctgt ggtgaacatc cagaaggaga ttgacagact gaatgaggtg 4620 gctaagaacc tgaatgagtc cctgattgac ctccaagaac tgggcaaata tgaacaatac 4680 atcaagtggc catgaaaatt gatcataatc agccatacca catttgtaga ggttttactt 4740 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 4800 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 4860 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 4920 gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa gaatatataa ggtgggggtc 4980 ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc aactcgtttg 5040 atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc 5100 agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct 5160 tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag 5220 ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa 5280 gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat 5340 tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc 5400 aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac 5460 cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta ggggttttgc 5520 gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt attttttcca 5580 ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg tctctggggt 5640 ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg atccagtcgt 5700 agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg attgccaggg 5760 gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc atacgtgggg 5820 atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc atatccctcc 5880 ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg ggaaatttgt 5940 catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga cctccaagat 6000 tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc tgggcgaaga 6060 tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca taggccattt 6120 ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc ggcccagggg 6180 cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg gggatcatgt 6240 ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc tgggaagaaa 6300 gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc acacctatta 6360 ccggctgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc aggggggcca 6420 cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc agaaggcgct 6480 cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt ttgagaccgt 6540 ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc cacagctcgg 6600 tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt tggggcggct 6660 ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt ctttccacgg 6720 gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc cgggctgcgc 6780 gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc ggtcttcgcc 6840 ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct ccgcggcgtg 6900 gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt gcagactttt 6960 gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat ccgcgccgca 7020 ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt cggggtcaaa 7080 aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca tgagccggtg 7140 tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga gaggcctgtc 7200 ctcgagcggt gttccgcggt cctcctcgta tagaaactcg gaccactctg agacaaaggc 7260 tcgcgtccag gccagcacga aggaggctaa gtgggagggg tagcggtcgt tgtccactag 7320 ggggtccact cgctccaggg tgtgaagaca catgtcgccc tcttcggcat caaggaaggt 7380 gattggtttg taggtgtagg ccacgtgacc gggtgttcct gaaggggggc tataaaaggg 7440 ggtgggggcg cgttcgtcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg 7500 gggtgagtac tccctctgaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa 7560 aaacgaggag gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcatc 7620 catctggtca gaaaagacaa tctttttgtt gtcaagcttg gtggcaaacg acccgtagag 7680 ggcgttggac agcaacttgg cgatggagcg cagggtttgg tttttgtcgc gatcggcgcg 7740 ctccttggcc gcgatgttta gctgcacgta ttcgcgcgca acgcaccgcc attcgggaaa 7800 gacggtggtg cgctcgtcgg gcaccaggtg cacgcgccaa ccgcggttgt gcagggtgac 7860 aaggtcaacg ctggtggcta cctctccgcg taggcgctcg ttggtccagc agaggcggcc 7920 gcccttgcgc gagcagaatg gcggtagggg gtctagctgc gtctcgtccg gggggtctgc 7980 gtccacggta aagaccccgg gcagcaggcg cgcgtcgaag tagtctatct tgcatccttg 8040 caagtctagc gcctgctgcc atgcgcgggc ggcaagcgcg cgctcgtatg ggttgagtgg 8100 gggaccccat ggcatggggt gggtgagcgc ggaggcgtac atgccgcaaa tgtcgtaaac 8160 gtagaggggc tctctgagta ttccaagata tgtagggtag catcttccac cgcggatgct 8220 ggcgcgcacg taatcgtata gttcgtgcga gggagcgagg aggtcgggac cgaggttgct 8280 acgggcgggc tgctctgctc ggaagactat ctgcctgaag atggcatgtg agttggatga 8340 tatggttgga cgctggaaga cgttgaagct ggcgtctgtg agacctaccg cgtcacgcac 8400 gaaggaggcg taggagtcgc gcagcttgtt gaccagctcg gcggtgacct gcacgtctag 8460 ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct tttttttcca 8520 cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc 8580 gtcggcctcc gaacggtaag agcctagcat gtagaactgg ttgacggcct ggtaggcgca 8640 gcatcccttt tctacgggta gcgcgtatgc ctgcgcggcc ttccggagcg aggtgtgggt 8700 gagcgcaaag gtgtccctga ccatgacttt gaggtactgg tatttgaagt cagtgtcgtc 8760 gcatccgccc tgctcccaga gcaaaaagtc cgtgcgcttt ttggaacgcg gatttggcag 8820 ggcgaaggtg acatcgttga agagtatctt tcccgcgcga ggcataaagt tgcgtgtgat 8880 gcggaagggt cccggcacct cggaacggtt gttaattacc tgggcggcga gcacgatctc 8940 gtcaaagccg ttgatgttgt ggcccacaat gtaaagttcc aagaagcgcg ggatgccctt 9000 gatggaaggc aattttttaa gttcctcgta ggtgagctct tcaggggagc tgagcccgtg 9060 ctctgaaagg gcccagtctg caagatgagg gttggaagcg acgaatgagc tccacaggtc 9120 acgggccatt agcatttgca ggtggtcgcg aaaggtccta aactggcgac ctatggccat 9180 tttttctggg gtgatgcagt agaaggtaag cgggtcttgt tcccagcggt cccatccaag 9240 gttcgcggct aggtctcgcg cggcagtcac tagaggctca tctccgccga acttcatgac 9300 cagcatgaag ggcacgagct gcttcccaaa ggcccccatc caagtatagg tctctacatc 9360 gtaggtgaca aagagacgct cggtgcgagg atgcgagccg atcgggaaga actggatctc 9420 ccgccaccaa ttggaggagt ggctattgat gtggtgaaag tagaagtccc tgcgacgggc 9480 cgaacactcg tgctggcttt tgtaaaaacg tgcgcagtac tggcagcggt gcacgggctg 9540 tacatcctgc acgaggttga cctgacgacc gcgcacaagg aagcagagtg ggaatttgag 9600 cccctcgcct ggcgggtttg gctggtggtc ttctacttcg gctgcttgtc cttgaccgtc 9660 tggctgctcg aggggagtta cggtggatcg gaccaccacg ccgcgcgagc ccaaagtcca 9720 gatgtccgcg cgcggcggtc ggagcttgat gacaacatcg cgcagatggg agctgtccat 9780 ggtctggagc tcccgcggcg tcaggtcagg cgggagctcc tgcaggttta cctcgcatag 9840 acgggtcagg gcgcgggcta gatccaggtg atacctaatt tccaggggct ggttggtggc 9900 ggcgtcgatg gcttgcaaga ggccgcatcc ccgcggcgcg actacggtac cgcgcggcgg 9960 gcggtgggcc gcgggggtgt ccttggatga tgcatctaaa agcggtgacg cgggcgagcc 10020 cccggaggta gggggggctc cggacccgcc gggagagggg gcaggggcac gtcggcgccg 10080 cgcgcgggca ggagctggtg ctgcgcgcgt aggttgctgg cgaacgcgac gacgcggcgg 10140 ttgatctcct gaatctggcg cctctgcgtg aagacgacgg gcccggtgag cttgaacctg 10200 aaagagagtt cgacagaatc aatttcggtg tcgttgacgg cggcctggcg caaaatctcc 10260 tgcacgtctc ctgagttgtc ttgataggcg atctcggcca tgaactgctc gatctcttcc 10320 tcctggagat ctccgcgtcc ggctcgctcc acggtggcgg cgaggtcgtt ggaaatgcgg 10380 gccatgagct gcgagaaggc gttgaggcct ccctcgttcc agacgcggct gtagaccacg 10440 cccccttcgg catcgcgggc gcgcatgacc acctgcgcga gattgagctc cacgtgccgg 10500 gcgaagacgg cgtagtttcg caggcgctga aagaggtagt tgagggtggt ggcggtgtgt 10560 tctgccacga agaagtacat aacccagcgt cgcaacgtgg attcgttgat atcccccaag 10620 gcctcaaggc gctccatggc ctcgtagaag tccacggcga agttgaaaaa ctgggagttg 10680 cgcgccgaca cggttaactc ctcctccaga agacggatga gctcggcgac agtgtcgcgc 10740 acctcgcgct caaaggctac aggggcctct tcttcttctt caatctcctc ttccataagg 10800 gcctcccctt cttcttcttc tggcggcggt gggggagggg ggacacggcg gcgacgacgg 10860 cgcaccggga ggcggtcgac aaagcgctcg atcatctccc cgcggcgacg gcgcatggtc 10920 tcggtgacgg cgcggccgtt ctcgcggggg cgcagttgga agacgccgcc cgtcatgtcc 10980 cggttatggg ttggcggggg gctgccatgc ggcagggata cggcgctaac gatgcatctc 11040 aacaattgtt gtgtaggtac tccgccgccg agggacctga gcgagtccgc atcgaccgga 11100 tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc 11160 gtggcgggcg gcagcgggcg gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg 11220 taattaaagt aggcggtctt gagacggcgg atggtcgaca gaagcaccat gtccttgggt 11280 ccggcctgct gaatgcgcag gcggtcggcc atgccccagg cttcgttttg acatcggcgc 11340 aggtctttgt agtagtcttg catgagcctt tctaccggca cttcttcttc tccttcctct 11400 tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg agtttggccg taggtggcgc 11460 cctcttcctc ccatgcgtgt gaccccgaag cccctcatcg gctgaagcag ggctaggtcg 11520 gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg tgagggtaga ctggaagtca 11580 tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca gttggccata 11640 acggaccagt taacggtctg gtgacccggc tgcgagagct cggtgtacct gagacgcgag 11700 taagccctcg agtcaaatac gtagtcgttg caagtccgca ccaggtactg gtatcccacc 11760 aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg ggctccgggg 11820 gcgagatctt ccaacataag gcgatgatat ccgtagatgt acctggacat ccaggtgatg 11880 ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat gttgcgcagc 11940 ggcaaaaagt gctccatggt cgggacgctc tggccggtca ggcgcgcgca atcgttgacg 12000 ctctagcgtg caaaaggaga gcctgtaagc gggcactctt ccgtggtctg gtggataaat 12060 tcgcaagggt atcatggcgg acgaccgggg ttcgagcccc gtatccggcc gtccgccgtg 12120 atccatgcgg ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac aacgggggag 12180 tgctcctttt ggcttccttc caggcgcggc ggctgctgcg ctagcttttt tggccactgg 12240 ccgcgcgcag cgtaagcggt taggctggaa agcgaaagca ttaagtggct cgctccctgt 12300 agccggaggg ttattttcca agggttgagt cgcgggaccc ccggttcgag tctcggaccg 12360 gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc ttgcaaattc 12420 ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg tgctgcggca 12480 gatgcgcccc cctcctcagc agcggcaaga gcaagagcag cggcagacat gcagggcacc 12540 ctcccctcct cctaccgcgt caggaggggc gacatccgcg gttgacgcgg cagcagatgg 12600 tgattacgaa cccccgcggc gccgggcccg gcactacctg gacttggagg agggcgaggg 12660 cctggcgcgg ctaggagcgc cctctcctga gcggcaccca agggtgcagc tgaagcgtga 12720 tacgcgtgag gcgtacgtgc cgcggcagaa cctgtttcgc gaccgcgagg gagaggagcc 12780 cgaggagatg cgggatcgaa agttccacgc agggcgcgag ctgcggcatg gcctgaatcg 12840 cgagcggttg ctgcgcgagg aggactttga gcccgacgcg cgaaccggga ttagtcccgc 12900 gcgcgcacac gtggcggccg ccgacctggt aaccgcatac gagcagacgg tgaaccagga 12960 gattaacttt caaaaaagct ttaacaacca cgtgcgtacg cttgtggcgc gcgaggaggt 13020 ggctatagga ctgatgcatc tgtgggactt tgtaagcgcg ctggagcaaa acccaaatag 13080 caagccgctc atggcgcagc tgttccttat agtgcagcac agcagggaca acgaggcatt 13140 cagggatgcg ctgctaaaca tagtagagcc cgagggccgc tggctgctcg atttgataaa 13200 catcctgcag agcatagtgg tgcaggagcg cagcttgagc ctggctgaca aggtggccgc 13260 catcaactat tccatgctta gcctgggcaa gttttacgcc cgcaagatat accatacccc 13320 ttacgttccc atagacaagg aggtaaagat cgaggggttc tacatgcgca tggcgctgaa 13380 ggtgcttacc ttgagcgacg acctgggcgt ttatcgcaac gagcgcatcc acaaggccgt 13440 gagcgtgagc cggcggcgcg agctcagcga ccgcgagctg atgcacagcc tgcaaagggc 13500 cctggctggc acgggcagcg gcgatagaga ggccgagtcc tactttgacg cgggcgctga 13560 cctgcgctgg gccccaagcc gacgcgccct ggaggcagct ggggccggac ctgggctggc 13620 ggtggcaccc gcgcgcgctg gcaacgtcgg cggcgtggag gaatatgacg aggacgatga 13680 gtacgagcca gaggacggcg agtactaagc ggtgatgttt ctgatcagat gatgcaagac 13740 gcaacggacc cggcggtgcg ggcggcgctg cagagccagc cgtccggcct taactccacg 13800 gacgactggc gccaggtcat ggaccgcatc atgtcgctga ctgcgcgcaa tcctgacgcg 13860 ttccggcagc agccgcaggc caaccggctc tccgcaattc tggaagcggt ggtcccggcg 13920 cgcgcaaacc ccacgcacga gaaggtgctg gcgatcgtaa acgcgctggc cgaaaacagg 13980 gccatccggc ccgacgaggc cggcctggtc tacgacgcgc tgcttcagcg cgtggctcgt 14040 tacaacagcg gcaacgtgca gaccaacctg gaccggctgg tgggggatgt gcgcgaggcc 14100 gtggcgcagc gtgagcgcgc gcagcagcag ggcaacctgg gctccatggt tgcactaaac 14160 gccttcctga gtacacagcc cgccaacgtg ccgcggggac aggaggacta caccaacttt 14220 gtgagcgcac tgcggctaat ggtgactgag acaccgcaaa gtgaggtgta ccagtctggg 14280 ccagactatt ttttccagac cagtagacaa ggcctgcaga ccgtaaacct gagccaggct 14340 ttcaaaaact tgcaggggct gtggggggtg cgggctccca caggcgaccg cgcgaccgtg 14400 tctagcttgc tgacgcccaa ctcgcgcctg ttgctgctgc taatagcgcc cttcacggac 14460 agtggcagcg tgtcccggga cacataccta ggtcacttgc tgacactgta ccgcgaggcc 14520 ataggtcagg cgcatgtgga cgagcatact ttccaggaga ttacaagtgt cagccgcgcg 14580 ctggggcagg aggacacggg cagcctggag gcaaccctaa actacctgct gaccaaccgg 14640 cggcagaaga tcccctcgtt gcacagttta aacagcgagg aggagcgcat tttgcgctac 14700 gtgcagcaga gcgtgagcct taacctgatg cgcgacgggg taacgcccag cgtggcgctg 14760 gacatgaccg cgcgcaacat ggaaccgggc atgtatgcct caaaccggcc gtttatcaac 14820 cgcctaatgg actacttgca tcgcgcggcc gccgtgaacc ccgagtattt caccaatgcc 14880 atcttgaacc cgcactggct accgccccct ggtttctaca ccgggggatt cgaggtgccc 14940 gagggtaacg atggattcct ctgggacgac atagacgaca gcgtgttttc cccgcaaccg 15000 cagaccctgc tagagttgca acagcgcgag caggcagagg cggcgctgcg aaaggaaagc 15060 ttccgcaggc caagcagctt gtccgatcta ggcgctgcgg ccccgcggtc agatgctagt 15120 agcccatttc caagcttgat agggtctctt accagcactc gcaccacccg cccgcgcctg 15180 ctgggcgagg aggagtacct aaacaactcg ctgctgcagc cgcagcgcga aaaaaacctg 15240 cctccggcat ttcccaacaa cgggatagag agcctagtgg acaagatgag tagatggaag 15300 acgtacgcgc aggagcacag ggacgtgcca ggcccgcgcc cgcccacccg tcgtcaaagg 15360 cacgaccgtc agcggggtct ggtgtgggag gacgatgact cggcagacga cagcagcgtc 15420 ctggatttgg gagggagtgg caacccgttt gcgcaccttc gccccaggct ggggagaatg 15480 ttttaaaaaa aaaaaagcat gatgcaaaat aaaaaactca ccaaggccat ggcaccgagc 15540 gttggttttc ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag gaaggtcctc 15600 ctccctccta cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg ggttctccct 15660 tcgatgctcc cctggacccg ccgtttgtgc ctccgcggta cctgcggcct accgggggga 15720 gaaacagcat ccgttactct gagttggcac ccctattcga caccacccgt gtgtacctgg 15780 tggacaacaa gtcaacggat gtggcatccc tgaactacca gaacgaccac agcaactttc 15840 tgaccacggt cattcaaaac aatgactaca gcccggggga ggcaagcaca cagaccatca 15900 atcttgacga ccggtcgcac tggggcggcg acctgaaaac catcctgcat accaacatgc 15960 caaatgtgaa cgagttcatg tttaccaata agtttaaggc gcgggtgatg gtgtcgcgct 16020 tgcctactaa ggacaatcag gtggagctga aatacgagtg ggtggagttc acgctgcccg 16080 agggcaacta ctccgagacc atgaccatag accttatgaa caacgcgatc gtggagcact 16140 acttgaaagt gggcagacag aacggggttc tggaaagcga catcggggta aagtttgaca 16200 cccgcaactt cagactgggg tttgaccccg tcactggtct tgtcatgcct ggggtatata 16260 caaacgaagc cttccatcca gacatcattt tgctgccagg atgcggggtg gacttcaccc 16320 acagccgcct gagcaacttg ttgggcatcc gcaagcggca acccttccag gagggcttta 16380 ggatcaccta cgatgatctg gagggtggta acattcccgc actgttggat gtggacgcct 16440 accaggcgag cttgaaagat gacaccgaac agggcggggg tggcgcaggc ggcagcaaca 16500 gcagtggcag cggcgcggaa gagaactcca acgcggcagc cgcggcaatg cagccggtgg 16560 aggacatgaa cgatcatgcc attcgcggcg acacctttgc cacacgggct gaggagaagc 16620 gcgctgaggc cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc gaggtcgaga 16680 agcctcagaa gaaaccggtg atcaaacccc tgacagagga cagcaagaaa cgcagttaca 16740 acctaataag caatgacagc accttcaccc agtaccgcag ctggtacctt gcatacaact 16800 acggcgaccc tcagaccgga atccgctcat ggaccctgct ttgcactcct gacgtaacct 16860 gcggctcgga gcaggtctac tggtcgttgc cagacatgat gcaagacccc gtgaccttcc 16920 gctccacgcg ccagatcagc aactttccgg tggtgggcgc cgagctgttg cccgtgcact 16980 ccaagagctt ctacaacgac caggccgtct actcccaact catccgccag tttacctctc 17040 tgacccacgt gttcaatcgc tttcccgaga accagatttt ggcgcgcccg ccagccccca 17100 ccatcaccac cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg ctaccgctgc 17160 gcaacagcat cggaggagtc cagcgagtga ccattactga cgccagacgc cgcacctgcc 17220 cctacgttta caaggccctg ggcatagtct cgccgcgcgt cctatcgagc cgcacttttt 17280 gagcaagcat gtccatcctt atatcgccca gcaataacac aggctggggc ctgcgcttcc 17340 caagcaagat gtttggcggg gccaagaagc gctccgacca acacccagtg cgcgtgcgcg 17400 ggcactaccg cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc accaccgtcg 17460 atgacgccat cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg ccgccaccag 17520 tgtccacagt ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc tatgctaaaa 17580 tgaagagacg gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc actgccgccc 17640 aacgcgcggc ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg gcggccatgc 17700 gggccgctcg aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc aggcgacgag 17760 cggccgccgc agcagccgcg gccattagtg ctatgactca gggtcgcagg ggcaacgtgt 17820 attgggtgcg cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc cccccgcgca 17880 actagattgc aagaaaaaac tacttagact cgtactgttg tatgtatcca gcggcggcgg 17940 cgcgcaacga agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag gtcatcgcgc 18000 cggagatcta tggccccccg aagaaggaag agcaggatta caagccccga aagctaaagc 18060 gggtcaaaaa gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg gaactgctgc 18120 acgctaccgc gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa cgtgttttgc 18180 gacccggcac caccgtagtc tttacgcccg gtgagcgctc cacccgcacc tacaagcgcg 18240 tgtatgatga ggtgtacggc gacgaggacc tgcttgagca ggccaacgag cgcctcgggg 18300 agtttgccta cggaaagcgg cataaggaca tgctggcgtt gccgctggac gagggcaacc 18360 caacacctag cctaaagccc gtaacactgc agcaggtgct gcccgcgctt gcaccgtccg 18420 aagaaaagcg cggcctaaag cgcgagtctg gtgacttggc acccaccgtg cagctgatgg 18480 tacccaagcg ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa cctgggctgg 18540 agcccgaggt ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc gtgcagaccg 18600 tggacgttca gatacccact accagtagca ccagtattgc caccgccaca gagggcatgg 18660 agacacaaac gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag gcggtcgctg 18720 cggccgcgtc caagacctct acggaggtgc aaacggaccc gtggatgttt cgcgtttcag 18780 ccccccggcg cccgcgccgt tcgaggaagt acggcgccgc cagcgcgcta ctgcccgaat 18840 atgccctaca tccttccatt gcgcctaccc ccggctatcg tggctacacc taccgcccca 18900 gaagacgagc aactacccga cgccgaacca ccactggaac ccgccgccgc cgtcgccgtc 18960 gccagcccgt gctggccccg atttccgtgc gcagggtggc tcgcgaagga ggcaggaccc 19020 tggtgctgcc aacagcgcgc taccacccca gcatcgttta aaagccggtc tttgtggttc 19080 ttgcagatat ggccctcacc tgccgcctcc gtttcccggt gccgggattc cgaggaagaa 19140 tgcaccgtag gaggggcatg gccggccacg gcctgacggg cggcatgcgt cgtgcgcacc 19200 accggcggcg gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc ctccttattc 19260 cactgatcgc cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc ttgcaggcgc 19320 agagacactg attaaaaaca agttgcatgt ggaaaaatca aaataaaaag tctggactct 19380 cacgctcgct tggtcctgta actattttgt agaatggaag acatcaactt tgcgtctctg 19440 gccccgcgac acggctcgcg cccgttcatg ggaaactggc aagatatcgg caccagcaat 19500 atgagcggtg gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa tttcggttcc 19560 accgttaaga actatggcag caaggcctgg aacagcagca caggccagat gctgagggat 19620 aagttgaaag agcaaaattt ccaacaaaag gtggtagatg gcctggcctc tggcattagc 19680 ggggtggtgg acctggccaa ccaggcagtg caaaataaga ttaacagtaa gcttgatccc 19740 cgccctcccg tagaggagcc tccaccggcc gtggagacag tgtctccaga ggggcgtggc 19800 gaaaagcgtc cgcgccccga cagggaagaa actctggtga cgcaaataga cgagcctccc 19860 tcgtacgagg aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc gcccatggct 19920 accggagtgc tgggccagca cacacccgta acgctggacc tgcctccccc cgccgacacc 19980 cagcagaaac ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc tagccgcgcg 20040 tccctgcgcc gcgccgccag cggtccgcga tcgttgcggc ccgtagccag tggcaactgg 20100 caaagcacac tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg ccgacgatgc 20160 ttctgatagc taacgtgtcg tatgtgtgtc atgtatgcgt ccatgtcgcc gccagaggag 20220 ctgctgagcc gccgcgcgcc cgctttccaa gatggctacc ccttcgatga tgccgcagtg 20280 gtcttacatg cacatctcgg gccaggacgc ctcggagtac ctgagccccg ggctggtgca 20340 gtttgcccgc gccaccgaga cgtacttcag cctgaataac aagtttagaa accccacggt 20400 ggcgcctacg cacgacgtga ccacagaccg gtcccagcgt ttgacgctgc ggttcatccc 20460 tgtggaccgt gaggatactg cgtactcgta caaggcgcgg ttcaccctag ctgtgggtga 20520 taaccgtgtg ctggacatgg cttccacgta ctttgacatc cgcggcgtgc tggacagggg 20580 ccctactttt aagccctact ctggcactgc ctacaacgcc ctggctccca agggtgcccc 20640 aaatccttgc gaatgggatg aagctgctac tgctcttgaa ataaacctag aagaagagga 20700 cgatgacaac gaagacgaag tagacgagca agctgagcag caaaaaactc acgtatttgg 20760 gcaggcgcct tattctggta taaatattac aaaggagggt attcaaatag gtgtcgaagg 20820 tcaaacacct aaatatgccg ataaaacatt tcaacctgaa cctcaaatag gagaatctca 20880 gtggtacgaa acagaaatta atcatgcagc tgggagagtc ctaaaaaaga ctaccccaat 20940 gaaaccatgt tacggttcat atgcaaaacc cacaaatgaa aatggagggc aaggcattct 21000 tgtaaagcaa caaaatggaa agctagaaag tcaagtggaa atgcaatttt tctcaactac 21060 tgaggcagcc gcaggcaatg gtgataactt gactcctaaa gtggtattgt acagtgaaga 21120 tgtagatata gaaaccccag acactcatat ttcttacatg cccactatta aggaaggtaa 21180 ctcacgagaa ctaatgggcc aacaatctat gcccaacagg cctaattaca ttgcttttag 21240 ggacaatttt attggtctaa tgtattacaa cagcacgggt aatatgggtg ttctggcggg 21300 ccaagcatcg cagttgaatg ctgttgtaga tttgcaagac agaaacacag agctttcata 21360 ccagcttttg cttgattcca ttggtgatag aaccaggtac ttttctatgt ggaatcaggc 21420 tgttgacagc tatgatccag atgttagaat tattgaaaat catggaactg aagatgaact 21480 tccaaattac tgctttccac tgggaggtgt gattaataca gagactctta ccaaggtaaa 21540 acctaaaaca ggtcaggaaa atggatggga aaaagatgct acagaatttt cagataaaaa 21600 tgaaataaga gttggaaata attttgccat ggaaatcaat ctaaatgcca acctgtggag 21660 aaatttcctg tactccaaca tagcgctgta tttgcccgac aagctaaagt acagtccttc 21720 caacgtaaaa atttctgata acccaaacac ctacgactac atgaacaagc gagtggtggc 21780 tcccgggcta gtggactgct acattaacct tggagcacgc tggtcccttg actatatgga 21840 caacgtcaac ccatttaacc accaccgcaa tgctggcctg cgctaccgct caatgttgct 21900 gggcaatggt cgctatgtgc ccttccacat ccaggtgcct cagaagttct ttgccattaa 21960 aaacctcctt ctcctgccgg gctcatacac ctacgagtgg aacttcagga aggatgttaa 22020 catggttctg cagagctccc taggaaatga cctaagggtt gacggagcca gcattaagtt 22080 tgatagcatt tgcctttacg ccaccttctt ccccatggcc cacaacaccg cctccacgct 22140 tgaggccatg cttagaaacg acaccaacga ccagtccttt aacgactatc tctccgccgc 22200 caacatgctc taccctatac ccgccaacgc taccaacgtg cccatatcca tcccctcccg 22260 caactgggcg gctttccgcg gctgggcctt cacgcgcctt aagactaagg aaaccccatc 22320 actgggctcg ggctacgacc cttattacac ctactctggc tctataccct acctagatgg 22380 aaccttttac ctcaaccaca cctttaagaa ggtggccatt acctttgact cttctgtcag 22440 ctggcctggc aatgaccgcc tgcttacccc caacgagttt gaaattaagc gctcagttga 22500 cggggagggt tacaacgttg cccagtgtaa catgaccaaa gactggttcc tggtacaaat 22560 gctagctaac tataacattg gctaccaggg cttctatatc ccagagagct acaaggaccg 22620 catgtactcc ttctttagaa acttccagcc catgagccgt caggtggtgg atgatactaa 22680 atacaaggac taccaacagg tgggcatcct acaccaacac aacaactctg gatttgttgg 22740 ctaccttgcc cccaccatgc gcgaaggaca ggcctaccct gctaacttcc cctatccgct 22800 tataggcaag accgcagttg acagcattac ccagaaaaag tttctttgcg atcgcaccct 22860 ttggcgcatc ccattctcca gtaactttat gtccatgggc gcactcacag acctgggcca 22920 aaaccttctc tacgccaact ccgcccacgc gctagacatg acttttgagg tggatcccat 22980 ggacgagccc acccttcttt atgttttgtt tgaagtcttt gacgtggtcc gtgtgcacca 23040 gccgcaccgc ggcgtcatcg aaaccgtgta cctgcgcacg cccttctcgg ccggcaacgc 23100 cacaacataa agaagcaagc aacatcaaca acagctgccg ccatgggctc cagtgagcag 23160 gaactgaaag ccattgtcaa agatcttggt tgtgggccat attttttggg cacctatgac 23220 aagcgctttc caggctttgt ttctccacac aagctcgcct gcgccatagt caatacggcc 23280 ggtcgcgaga ctgggggcgt acactggatg gcctttgcct ggaacccgca ctcaaaaaca 23340 tgctacctct ttgagccctt tggcttttct gaccagcgac tcaagcaggt ttaccagttt 23400 gagtacgagt cactcctgcg ccgtagcgcc attgcttctt cccccgaccg ctgtataacg 23460 ctggaaaagt ccacccaaag cgtacagggg cccaactcgg ccgcctgtgg actattctgc 23520 tgcatgtttc tccacgcctt tgccaactgg ccccaaactc ccatggatca caaccccacc 23580 atgaacctta ttaccggggt acccaactcc atgctcaaca gtccccaggt acagcccacc 23640 ctgcgtcgca accaggaaca gctctacagc ttcctggagc gccactcgcc ctacttccgc 23700 agccacagtg cgcagattag gagcgccact tctttttgtc acttgaaaaa catgtaaaaa 23760 taatgtacta gagacacttt caataaaggc aaatgctttt atttgtacac tctcgggtga 23820 ttatttaccc ccacccttgc cgtctgcgcc gtttaaaaat caaaggggtt ctgccgcgca 23880 tcgctatgcg ccactggcag ggacacgttg cgatactggt gtttagtgct ccacttaaac 23940 tcaggcacaa ccatccgcgg cagctcggtg aagttttcac tccacaggct gcgcaccatc 24000 accaacgcgt ttagcaggtc gggcgccgat atcttgaagt cgcagttggg gcctccgccc 24060 tgcgcgcgcg agttgcgata cacagggttg cagcactgga acactatcag cgccgggtgg 24120 tgcacgctgg ccagcacgct cttgtcggag atcagatccg cgtccaggtc ctccgcgttg 24180 ctcagggcga acggagtcaa ctttggtagc tgccttccca aaaagggcgc gtgcccaggc 24240 tttgagttgc actcgcaccg tagtggcatc aaaaggtgac cgtgcccggt ctgggcgtta 24300 ggatacagcg cctgcataaa agccttgatc tgcttaaaag ccacctgagc ctttgcgcct 24360 tcagagaaga acatgccgca agacttgccg gaaaactgat tggccggaca ggccgcgtcg 24420 tgcacgcagc accttgcgtc ggtgttggag atctgcacca catttcggcc ccaccggttc 24480 ttcacgatct tggccttgct agactgctcc ttcagcgcgc gctgcccgtt ttcgctcgtc 24540 acatccattt caatcacgtg ctccttattt atcataatgc ttccgtgtag acacttaagc 24600 tcgccttcga tctcagcgca gcggtgcagc cacaacgcgc agcccgtggg ctcgtgatgc 24660 ttgtaggtca cctctgcaaa cgactgcagg tacgcctgca ggaatcgccc catcatcgtc 24720 acaaaggtct tgttgctggt gaaggtcagc tgcaacccgc ggtgctcctc gttcagccag 24780 gtcttgcata cggccgccag agcttccact tggtcaggca gtagtttgaa gttcgccttt 24840 agatcgttat ccacgtggta cttgtccatc agcgcgcgcg cagcctccat gcccttctcc 24900 cacgcagaca cgatcggcac actcagcggg ttcatcaccg taatttcact ttccgcttcg 24960 ctgggctctt cctcttcctc ttgcgtccgc ataccacgcg ccactgggtc gtcttcattc 25020 agccgccgca ctgtgcgctt acctcctttg ccatgcttga ttagcaccgg tgggttgctg 25080 aaacccacca tttgtagcgc cacatcttct ctttcttcct cgctgtccac gattacctct 25140 ggtgatggcg ggcgctcggg cttgggagaa gggcgcttct ttttcttctt gggcgcaatg 25200 gccaaatccg ccgccgaggt cgatggccgc gggctgggtg tgcgcggcac cagcgcgtct 25260 tgtgatgagt cttcctcgtc ctcggactcg atacgccgcc tcatccgctt ttttgggggc 25320 gcccggggag gcggcggcga cggggacggg gacgacacgt cctccatggt tgggggacgt 25380 cgcgccgcac cgcgtccgcg ctcgggggtg gtttcgcgct gctcctcttc ccgactggcc 25440 atttccttct cctataggca gaaaaagatc atggagtcag tcgagaagaa ggacagccta 25500 accgccccct ctgagttcgc caccaccgcc tccaccgatg ccgccaacgc gcctaccacc 25560 ttccccgtcg aggcaccccc gcttgaggag gaggaagtga ttatcgagca ggacccaggt 25620 tttgtaagcg aagacgacga ggaccgctca gtaccaacag aggataaaaa gcaagaccag 25680 gacaacgcag aggcaaacga ggaacaagtc gggcgggggg acgaaaggca tggcgactac 25740 ctagatgtgg gagacgacgt gctgttgaag catctgcagc gccagtgcgc cattatctgc 25800 gacgcgttgc aagagcgcag cgatgtgccc ctcgccatag cggatgtcag ccttgcctac 25860 gaacgccacc tattctcacc gcgcgtaccc cccaaacgcc aagaaaacgg cacatgcgag 25920 cccaacccgc gcctcaactt ctaccccgta tttgccgtgc cagaggtgct tgccacctat 25980 cacatctttt tccaaaactg caagataccc ctatcctgcc gtgccaaccg cagccgagcg 26040 gacaagcagc tggccttgcg gcagggcgct gtcatacctg atatcgcctc gctcaacgaa 26100 gtgccaaaaa tctttgaggg tcttggacgc gacgagaagc gcgcggcaaa cgctctgcaa 26160 caggaaaaca gcgaaaatga aagtcactct ggagtgttgg tggaactcga gggtgacaac 26220 gcgcgcctag ccgtactaaa acgcagcatc gaggtcaccc actttgccta cccggcactt 26280 aacctacccc ccaaggtcat gagcacagtc atgagtgagc tgatcgtgcg ccgtgcgcag 26340 cccctggaga gggatgcaaa tttgcaagaa caaacagagg agggcctacc cgcagttggc 26400 gacgagcagc tagcgcgctg gcttcaaacg cgcgagcctg ccgacttgga ggagcgacgc 26460 aaactaatga tggccgcagt gctcgttacc gtggagcttg agtgcatgca gcggttcttt 26520 gctgacccgg agatgcagcg caagctagag gaaacattgc actacacctt tcgacagggc 26580 tacgtacgcc aggcctgcaa gatctccaac gtggagctct gcaacctggt ctcctacctt 26640 ggaattttgc acgaaaaccg ccttgggcaa aacgtgcttc attccacgct caagggcgag 26700 gcgcgccgcg actacgtccg cgactgcgtt tacttatttc tatgctacac ctggcagacg 26760 gccatgggcg tttggcagca gtgcttggag gagtgcaacc tcaaggagct gcagaaactg 26820 ctaaagcaaa acttgaagga cctatggacg gccttcaacg agcgctccgt ggccgcgcac 26880 ctggcggaca tcattttccc cgaacgcctg cttaaaaccc tgcaacaggg tctgccagac 26940 ttcaccagtc aaagcatgtt gcagaacttt aggaacttta tcctagagcg ctcaggaatc 27000 ttgcccgcca cctgctgtgc acttcctagc gactttgtgc ccattaagta ccgcgaatgc 27060 cctccgccgc tttggggcca ctgctacctt ctgcagctag ccaactacct tgcctaccac 27120 tctgacataa tggaagacgt gagcggtgac ggtctactgg agtgtcactg tcgctgcaac 27180 ctatgcaccc cgcaccgctc cctggtttgc aattcgcagc tgcttaacga aagtcaaatt 27240 atcggtacct ttgagctgca gggtccctcg cctgacgaaa agtccgcggc tccggggttg 27300 aaactcactc cggggctgtg gacgtcggct taccttcgca aatttgtacc tgaggactac 27360 cacgcccacg agattaggtt ctacgaagac caatcccgcc cgcctaatgc ggagcttacc 27420 gcctgcgtca ttacccaggg ccacattctt ggccaattgc aagccatcaa caaagcccgc 27480 caagagtttc tgctacgaaa gggacggggg gtttacttgg acccccagtc cggcgaggag 27540 ctcaacccaa tccccccgcc gccgcagccc tatcagcagc agccgcgggc ccttgcttcc 27600 caggatggca cccaaaaaga agctgcagct gccgccgcca cccacggacg aggaggaata 27660 ctgggacagt caggcagagg aggttttgga cgaggaggag gaggacatga tggaagactg 27720 ggagagccta gacgaggaag cttccgaggt cgaagaggtg tcagacgaaa caccgtcacc 27780 ctcggtcgca ttcccctcgc cggcgcccca gaaatcggca accggttcca gcatggctac 27840 aacctccgct cctcaggcgc cgccggcact gcccgttcgc cgacccaacc gtagatggga 27900 caccactgga accagggccg gtaagtccaa gcagccgccg ccgttagccc aagagcaaca 27960 acagcgccaa ggctaccgct catggcgcgg gcacaagaac gccatagttg cttgcttgca 28020 agactgtggg ggcaacatct ccttcgcccg ccgctttctt ctctaccatc acggcgtggc 28080 cttcccccgt aacatcctgc attactaccg tcatctctac agcccatact gcaccggcgg 28140 cagcggcagc aacagcagcg gccacacaga agcaaaggcg accggatagc aagactctga 28200 caaagcccaa gaaatccaca gcggcggcag cagcaggagg aggagcgctg cgtctggcgc 28260 ccaacgaacc cgtatcgacc cgcgagctta gaaacaggat ttttcccact ctgtatgcta 28320 tatttcaaca gagcaggggc caagaacaag agctgaaaat aaaaaacagg tctctgcgat 28380 ccctcacccg cagctgcctg tatcacaaaa gcgaagatca gcttcggcgc acgctggaag 28440 acgcggaggc tctcttcagt aaatactgcg cgctgactct taaggactag tttcgcgccc 28500 tttctcaaat ttaagcgcga aaactacgtc atctccagcg gccacacccg gcgccagcac 28560 ctgttgtcag cgccattatg agcaaggaaa ttcccacgcc ctacatgtgg agttaccagc 28620 cacaaatggg acttgcggct ggagctgccc aagactactc aacccgaata aactacatga 28680 gcgcgggacc ccacatgata tcccgggtca acggaatacg cgcccaccga aaccgaattc 28740 tcctggaaca ggcggctatt accaccacac ctcgtaataa ccttaatccc cgtagttggc 28800 ccgctgccct ggtgtaccag gaaagtcccg ctcccaccac tgtggtactt cccagagacg 28860 cccaggccga agttcagatg actaactcag gggcgcagct tgcgggcggc tttcgtcaca 28920 gggtgcggtc gcccgggcag ggtataactc acctgacaat cagagggcga ggtattcagc 28980 tcaacgacga gtcggtgagc tcctcgcttg gtctccgtcc ggacgggaca tttcagatcg 29040 gcggcgccgg ccgctcttca ttcacgcctc gtcaggcaat cctaactctg cagacctcgt 29100 cctctgagcc gcgctctgga ggcattggaa ctctgcaatt tattgaggag tttgtgccat 29160 cggtctactt taaccccttc tcgggacctc ccggccacta tccggatcaa tttattccta 29220 actttgacgc ggtaaaggac tcggcggacg gctacgactg aatgttaagt ggagaggcag 29280 agcaactgcg cctgaaacac ctggtccact gtcgccgcca caagtgcttt gcccgcgact 29340 ccggtgagtt ttgctacttt gaattgcccg aggatcatat cgagggcccg gcgcacggcg 29400 tccggcttac cgcccaggga gagcttgccc gtagcctgat tcgggagttt acccagcgcc 29460 ccctgctagt tgagcgggac aggggaccct gtgttctcac tgtgatttgc aactgtccta 29520 accctggatt acatcaagat cctctagtta atgtcaggtc gcctaagtcg attaactaga 29580 gtacccgggg atcttattcc ctttaactaa taaaaaaaaa taataaagca tcacttactt 29640 aaaatcagtt agcaaatttc tgtccagttt attcagcagc acctccttgc cctcctccca 29700 gctctggtat tgcagcttcc tcctggctgc aaactttctc cacaatctaa atggaatgtc 29760 agtttcctcc tgttcctgtc catccgcacc cactatcttc atgttgttgc agatgaagcg 29820 cgcaagaccg tctgaagata ccttcaaccc cgtgtatcca tatgacacgg aaaccggtcc 29880 tccaactgtg ccttttctta ctcctccctt tgtatccccc aatgggtttc aagagagtcc 29940 ccctggggta ctctctttgc gcctatccga acctctagtt acctccaatg gcatgcttgc 30000 gctcaaaatg ggcaacggcc tctctctgga cgaggccggc aaccttacct cccaaaatgt 30060 aaccactgtg agcccacctc tcaaaaaaac caagtcaaac ataaacctgg aaatatctgc 30120 acccctcaca gttacctcag aagccctaac tgtggctgcc gccgcacctc taatggtcgc 30180 gggcaacaca ctcaccatgc aatcacaggc cccgctaacc gtgcacgact ccaaacttag 30240 cattgccacc caaggacccc tcacagtgtc agaaggaaag ctagccctgc aaacatcagg 30300 ccccctcacc accaccgata gcagtaccct tactatcact gcctcacccc ctctaactac 30360 tgccactggt agcttgggca ttgacttgaa agagcccatt tatacacaaa atggaaaact 30420 aggactaaag tacggggctc ctttgcatgt aacagacgac ctaaacactt tgaccgtagc 30480 aactggtcca ggtgtgacta ttaataatac ttccttgcaa actaaagtta ctggagcctt 30540 gggttttgat tcacaaggca atatgcaact taatgtagca ggaggactaa ggattgattc 30600 tcaaaacaga cgccttatac ttgatgttag ttatccgttt gatgctcaaa accaactaaa 30660 tctaagacta ggacagggcc ctctttttat aaactcagcc cacaacttgg atattaacta 30720 caacaaaggc ctttacttgt ttacagcttc aaacaattcc aaaaagcttg aggttaacct 30780 aagcactgcc aaggggttga tgtttgacgc tacagccata gccattaatg caggagatgg 30840 gcttgaattt ggttcaccta atgcaccaaa cacaaatccc ctcaaaacaa aaattggcca 30900 tggcctagaa tttgattcaa acaaggctat ggttcctaaa ctaggaactg gccttagttt 30960 tgacagcaca ggtgccatta cagtaggaaa caaaaataat gataagctaa ctttgtggac 31020 cggaataaac cctccaccta actgtcaaat tgtggaaaac actaatacaa atgatggcaa 31080 acttacttta gtattagtaa aaaatggagg gcttgttaat ggctacgtgt ctctagttgg 31140 tgtatcagac actgtgaacc aaatgttcac acaaaagaca gcaaacatcc aattaagatt 31200 atattttgac tcttctggaa atctattaac tgaggaatca gacttaaaaa ttccacttaa 31260 aaataaatct tctacagcga ccagtgaaac tgtagccagc agcaaagcct ttatgccaag 31320 tactacagct tatcccttca acaccactac tagggatagt gaaaactaca ttcatggaat 31380 atgttactac atgactagtt atgatagaag tctatttccc ttgaacattt ctataatgct 31440 aaacagccgt atgatttctt ccaatgttgc ctatgccata caatttgaat ggaatctaaa 31500 tgcaagtgaa tctccagaaa gcaacatagc tacgctgacc acatccccct ttttcttttc 31560 ttacattaca gaagacgaca actaaagaat cgtttgtgtt atgtttcaac gtgtttattt 31620 ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata 31680 gcttatacag atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac 31740 ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca 31800 tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca 31860 aacgctcatc agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt 31920 ccagctgctg agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag 31980 aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct 32040 gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg 32100 cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg 32160 cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa 32220 tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag 32280 aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca 32340 cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata 32400 taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct 32460 gcccgccggc tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg 32520 actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca 32580 cgtgcataca cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa 32640 caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca 32700 cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag 32760 cgcgggtttc tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca 32820 accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc 32880 ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta 32940 gatcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg 33000 gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca 33060 gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg 33120 ggaagagctg gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa 33180 atgaagatct attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa 33240 agaacagata atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct 33300 cacgtccaag tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc 33360 agcaccttca accatgccca aataattctc atctcgccac cttctcaata tatctctaag 33420 caaatcccga atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt 33480 cagcctcaag cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga 33540 ttcaaaagcg gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc 33600 tgaacataat cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg 33660 acaaaagaac ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc 33720 ccgatgtaag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca 33780 ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag 33840 gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt 33900 ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta 33960 caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt 34020 aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag 34080 tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc 34140 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca 34200 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct 34260 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag 34320 ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac 34380 ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag 34440 gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa 34500 cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt 34560 tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac 34620 tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc 34680 caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatg 34736 <210> 28 <211> 37705 <212> DNA <213> Artificial Sequence <220> <223> plasmid #4 vector <400> 28 ttaattaaca tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 60 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 120 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 180 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 240 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 300 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 360 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 420 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 480 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 540 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 600 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 660 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 720 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 780 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 840 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 900 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 960 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1020 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1080 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1140 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1200 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1260 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1320 ttgttgccat tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca 1380 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 1440 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 1500 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 1560 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 1620 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 1680 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1740 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1800 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1860 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1920 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1980 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2040 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2100 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2160 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 2220 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2280 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2340 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2400 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2460 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 2520 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 2580 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 2640 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 2700 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 2760 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 2820 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2880 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 2940 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 3000 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 3060 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 3120 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 3180 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 3240 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta 3300 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 3360 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 3420 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 3480 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 3540 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 3600 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 3660 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 3720 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3780 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3840 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 3900 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 3960 gacaccggga ccgatccagc ctccgatttg ccaccatgtt tgtgttcctg gtgctgctgc 4020 cactggtgtc cagccagtgt gtgaacctga ccaccaggac ccaacttcct cctgcctaca 4080 ccaactcctt caccagggga gtctactacc ctgacaaggt gttcaggtcc tctgtgctgc 4140 acagcaccca ggacctgttc ctgccattct tcagcaatgt gacctggttc catgccatcc 4200 atgtgtctgg caccaatggc accaagaggt ttgacaaccc tgtgctgcca ttcaatgatg 4260 gagtctactt tgccagcaca gagaagagca acatcatcag gggctggatt tttggcacca 4320 ccctggacag caagacccag tccctgctga ttgtgaacaa tgccaccaat gtggtgatta 4380 aggtgtgtga gttccagttc tgtaatgacc cattcctggg agtctactac cacaagaaca 4440 acaagtcctg gatggagtct gagttcaggg tctactcctc tgccaacaac tgtacctttg 4500 aatatgtgag ccaaccattc ctgatggact tggagggcaa gcagggcaac ttcaagaacc 4560 tgagggagtt tgtgttcaag aacattgatg gctacttcaa gatttacagc aaacacacac 4620 caatcaacct ggtgagggac ctgccacagg gcttctctgc cttggaacca ctggtggacc 4680 tgccaattgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcctacc 4740 tgacacctgg agactcctcc tctggctgga cagcaggagc agcagcctac tatgtgggct 4800 acctccaacc aaggaccttc ctgctgaaat acaatgagaa tggcaccatc acagatgctg 4860 tggactgtgc cctggaccca ctgtctgaga ccaagtgtac cctgaaatcc ttcacagtgg 4920 agaagggcat ctaccagacc agcaacttca gggtccaacc aacagagagc attgtgaggt 4980 ttccaaacat caccaacctg tgtccatttg gagaggtgtt caatgccacc aggtttgcct 5040 ctgtctatgc ctggaacagg aagaggatta gcaactgtgt ggctgactac tctgtgctct 5100 acaactctgc ctccttcagc accttcaagt gttatggagt gagcccaacc aaactgaatg 5160 acctgtgttt caccaatgtc tatgctgact cctttgtgat taggggagat gaggtgagac 5220 agattgcccc tggacaaaca ggcaagattg ctgactacaa ctacaaactg cctgatgact 5280 tcacaggctg tgtgattgcc tggaacagca acaacctgga cagcaaggtg ggaggcaact 5340 acaactacct ctacagactg ttcaggaaga gcaacctgaa accatttgag agggacatca 5400 gcacagagat ttaccaggct ggcagcacac catgtaatgg agtggagggc ttcaactgtt 5460 actttccact ccaatcctat ggcttccaac caaccaatgg agtgggctac caaccataca 5520 gggtggtggt gctgtccttt gaactgctcc atgcccctgc cacagtgtgt ggaccaaaga 5580 agagcaccaa cctggtgaag aacaagtgtg tgaacttcaa cttcaatgga ctgacaggca 5640 caggagtgct gacagagagc aacaagaagt tcctgccatt ccaacagttt ggcagggaca 5700 ttgctgacac cacagatgct gtgagggacc cacagacctt ggagattctg gacatcacac 5760 catgttcctt tggaggagtg tctgtgatta cacctggcac caacaccagc aaccaggtgg 5820 ctgtgctcta ccaggatgtg aactgtactg aggtgcctgt ggctatccat gctgaccaac 5880 ttacaccaac ctggagggtc tacagcacag gcagcaatgt gttccagacc agggctggct 5940 gtctgattgg agcagagcat gtgaacaact cctatgagtg tgacatccca attggagcag 6000 gcatctgtgc ctcctaccag acccagaccg gtggcggtgg gtcgggcggt ggtgggtcgg 6060 gtggcggcgg ttccaggtct gtggcaagcc agagcatcat tgcctacaca atgagtctgg 6120 gagcagagaa ctctgtggct tacagcaaca acagcattgc catcccaacc aacttcacca 6180 tctctgtgac cacagagatt ctgcctgtga gtatgaccaa gacctctgtg gactgtacaa 6240 tgtatatctg tggagacagc acagagtgta gcaacctgct gctccaatat ggctccttct 6300 gtacccaact taacagggct ctgacaggca ttgctgtgga acaggacaag aacacccagg 6360 aggtgtttgc ccaggtgaag cagatttaca agacacctcc aatcaaggac tttggaggct 6420 tcaacttcag ccagattctg cctgacccaa gcaagccaag caagaggtcc ttcattgagg 6480 acctgctgtt caacaaggtg accctggctg atgctggctt catcaagcaa tatggagact 6540 gtctgggaga cattgctgcc agggacctga tttgtgccca gaagttcaat ggactgacag 6600 tgctgcctcc actgctgaca gatgagatga ttgcccaata cacctctgcc ctgctggctg 6660 gcaccatcac ctctggctgg acctttggag caggagcagc cctccaaatc ccatttgcta 6720 tgcagatggc ttacaggttc aatggcattg gagtgaccca gaatgtgctc tatgagaacc 6780 agaaactgat tgccaaccag ttcaactctg ccattggcaa gattcaggac tccctgtcca 6840 gcacagcctc tgccctgggc aaactccaag atgtggtgaa ccagaatgcc caggctctga 6900 acaccctggt gaagcaactt tccagcaact ttggagccat ctcctctgtg ctgaatgaca 6960 tcctgagcag actggacaag gtggaggctg aggtccagat tgacagactg attacaggca 7020 gactccaatc cctccaaacc tatgtgaccc aacaacttat cagggctgct gagattaggg 7080 catctgccaa cctggctgcc accaagatga gtgagtgtgt gctgggacaa agcaagaggg 7140 tggacttctg tggcaagggc taccacctga tgagttttcc acagtctgcc cctcatggag 7200 tggtgttcct gcatgtgacc tatgtgcctg cccaggagaa gaacttcacc acagcccctg 7260 ccatctgcca tgatggcaag gctcactttc caagggaggg agtgtttgtg agcaatggca 7320 cccactggtt tgtgacccag aggaacttct atgaaccaca gattatcacc acagacaaca 7380 cctttgtgtc tggcaactgt gatgtggtga ttggcattgt gaacaacaca gtctatgacc 7440 cactccaacc tgaactggac tccttcaagg aggaactgga caaatacttc aagaaccaca 7500 ccagccctga tgtggacctg ggagacatct ctggcatcaa tgcctctgtg gtgaacatcc 7560 agaaggagat tgacagactg aatgaggtgg ctaagaacct gaatgagtcc ctgattgacc 7620 tccaagaact gggcaaatat gaacaataca tcaagtggcc atgaaaattg atcataatca 7680 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 7740 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 7800 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 7860 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cggatctggg cgtggttaag 7920 ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt ttgcagcagc 7980 cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat atttgacaac 8040 gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca ttgatggtcg 8100 ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg gaacgccgtt 8160 ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg ggattgtgac 8220 tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat ccgcccgcga 8280 tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac ttaatgtcgt 8340 ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt cctcccctcc 8400 caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga tcaagcaagt 8460 gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc agcggtctcg 8520 gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct ggatgttcag 8580 atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag cttcatgctg 8640 cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt gcctaaaaat 8700 gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt ttacaaagcg 8760 gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact gtatttttag 8820 gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa ccaccagcac 8880 agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg cgtggaagaa 8940 cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa tgatggcaat 9000 gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt catagttgtg 9060 ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg tgccagactg 9120 cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt gcatttccca 9180 cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga aaacggtttc 9240 cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg acttaccgca 9300 gccggtgggc ccgtaaatca cacctattac cggctgcaac tggtagttaa gagagctgca 9360 gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga ctcgcatgtt 9420 ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt cttgcaagga 9480 agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga gcgtttgacc 9540 aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc gatccagcat 9600 atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg gtgctcgtcc 9660 agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt agtctgggtc 9720 acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag gctggtcctg 9780 ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg 9840 gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc cttggaggag 9900 gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc gagaaatacc 9960 gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca ttccacgagc 10020 caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt tttgatgcgt 10080 ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag gctgtccgtg 10140 tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc ctcctcgtat 10200 agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa ggaggctaag 10260 tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt gtgaagacac 10320 atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc cacgtgaccg 10380 ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc actctcttcc 10440 gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa agcgggcatg 10500 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc 10560 gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat ctttttgttg 10620 tcaagcttgg tggcaaacga cccgtagagg gcgttggaca gcaacttggc gatggagcgc 10680 agggtttggt ttttgtcgcg atcggcgcgc tccttggccg cgatgtttag ctgcacgtat 10740 tcgcgcgcaa cgcaccgcca ttcgggaaag acggtggtgc gctcgtcggg caccaggtgc 10800 acgcgccaac cgcggttgtg cagggtgaca aggtcaacgc tggtggctac ctctccgcgt 10860 aggcgctcgt tggtccagca gaggcggccg cccttgcgcg agcagaatgg cggtaggggg 10920 tctagctgcg tctcgtccgg ggggtctgcg tccacggtaa agaccccggg cagcaggcgc 10980 gcgtcgaagt agtctatctt gcatccttgc aagtctagcg cctgctgcca tgcgcgggcg 11040 gcaagcgcgc gctcgtatgg gttgagtggg ggaccccatg gcatggggtg ggtgagcgcg 11100 gaggcgtaca tgccgcaaat gtcgtaaacg tagaggggct ctctgagtat tccaagatat 11160 gtagggtagc atcttccacc gcggatgctg gcgcgcacgt aatcgtatag ttcgtgcgag 11220 ggagcgagga ggtcgggacc gaggttgcta cgggcgggct gctctgctcg gaagactatc 11280 tgcctgaaga tggcatgtga gttggatgat atggttggac gctggaagac gttgaagctg 11340 gcgtctgtga gacctaccgc gtcacgcacg aaggaggcgt aggagtcgcg cagcttgttg 11400 accagctcgg cggtgacctg cacgtctagg gcgcagtagt ccagggtttc cttgatgatg 11460 tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg 11520 tctttccagt actcttggat cggaaacccg tcggcctccg aacggtaaga gcctagcatg 11580 tagaactggt tgacggcctg gtaggcgcag catccctttt ctacgggtag cgcgtatgcc 11640 tgcgcggcct tccggagcga ggtgtgggtg agcgcaaagg tgtccctgac catgactttg 11700 aggtactggt atttgaagtc agtgtcgtcg catccgccct gctcccagag caaaaagtcc 11760 gtgcgctttt tggaacgcgg atttggcagg gcgaaggtga catcgttgaa gagtatcttt 11820 cccgcgcgag gcataaagtt gcgtgtgatg cggaagggtc ccggcacctc ggaacggttg 11880 ttaattacct gggcggcgag cacgatctcg tcaaagccgt tgatgttgtg gcccacaatg 11940 taaagttcca agaagcgcgg gatgcccttg atggaaggca attttttaag ttcctcgtag 12000 gtgagctctt caggggagct gagcccgtgc tctgaaaggg cccagtctgc aagatgaggg 12060 ttggaagcga cgaatgagct ccacaggtca cgggccatta gcatttgcag gtggtcgcga 12120 aaggtcctaa actggcgacc tatggccatt ttttctgggg tgatgcagta gaaggtaagc 12180 gggtcttgtt cccagcggtc ccatccaagg ttcgcggcta ggtctcgcgc ggcagtcact 12240 agaggctcat ctccgccgaa cttcatgacc agcatgaagg gcacgagctg cttcccaaag 12300 gcccccatcc aagtataggt ctctacatcg taggtgacaa agagacgctc ggtgcgagga 12360 tgcgagccga tcgggaagaa ctggatctcc cgccaccaat tggaggagtg gctattgatg 12420 tggtgaaagt agaagtccct gcgacgggcc gaacactcgt gctggctttt gtaaaaacgt 12480 gcgcagtact ggcagcggtg cacgggctgt acatcctgca cgaggttgac ctgacgaccg 12540 cgcacaagga agcagagtgg gaatttgagc ccctcgcctg gcgggtttgg ctggtggtct 12600 tctacttcgg ctgcttgtcc ttgaccgtct ggctgctcga ggggagttac ggtggatcgg 12660 accaccacgc cgcgcgagcc caaagtccag atgtccgcgc gcggcggtcg gagcttgatg 12720 acaacatcgc gcagatggga gctgtccatg gtctggagct cccgcggcgt caggtcaggc 12780 gggagctcct gcaggtttac ctcgcataga cgggtcaggg cgcgggctag atccaggtga 12840 tacctaattt ccaggggctg gttggtggcg gcgtcgatgg cttgcaagag gccgcatccc 12900 cgcggcgcga ctacggtacc gcgcggcggg cggtgggccg cgggggtgtc cttggatgat 12960 gcatctaaaa gcggtgacgc gggcgagccc ccggaggtag ggggggctcc ggacccgccg 13020 ggagaggggg caggggcacg tcggcgccgc gcgcgggcag gagctggtgc tgcgcgcgta 13080 ggttgctggc gaacgcgacg acgcggcggt tgatctcctg aatctggcgc ctctgcgtga 13140 agacgacggg cccggtgagc ttgaacctga aagagagttc gacagaatca atttcggtgt 13200 cgttgacggc ggcctggcgc aaaatctcct gcacgtctcc tgagttgtct tgataggcga 13260 tctcggccat gaactgctcg atctcttcct cctggagatc tccgcgtccg gctcgctcca 13320 cggtggcggc gaggtcgttg gaaatgcggg ccatgagctg cgagaaggcg ttgaggcctc 13380 cctcgttcca gacgcggctg tagaccacgc ccccttcggc atcgcgggcg cgcatgacca 13440 cctgcgcgag attgagctcc acgtgccggg cgaagacggc gtagtttcgc aggcgctgaa 13500 agaggtagtt gagggtggtg gcggtgtgtt ctgccacgaa gaagtacata acccagcgtc 13560 gcaacgtgga ttcgttgata tcccccaagg cctcaaggcg ctccatggcc tcgtagaagt 13620 ccacggcgaa gttgaaaaac tgggagttgc gcgccgacac ggttaactcc tcctccagaa 13680 gacggatgag ctcggcgaca gtgtcgcgca cctcgcgctc aaaggctaca ggggcctctt 13740 cttcttcttc aatctcctct tccataaggg cctccccttc ttcttcttct ggcggcggtg 13800 ggggaggggg gacacggcgg cgacgacggc gcaccgggag gcggtcgaca aagcgctcga 13860 tcatctcccc gcggcgacgg cgcatggtct cggtgacggc gcggccgttc tcgcgggggc 13920 gcagttggaa gacgccgccc gtcatgtccc ggttatgggt tggcgggggg ctgccatgcg 13980 gcagggatac ggcgctaacg atgcatctca acaattgttg tgtaggtact ccgccgccga 14040 gggacctgag cgagtccgca tcgaccggat cggaaaacct ctcgagaaag gcgtctaacc 14100 agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg cagcgggcgg cggtcggggt 14160 tgtttctggc ggaggtgctg ctgatgatgt aattaaagta ggcggtcttg agacggcgga 14220 tggtcgacag aagcaccatg tccttgggtc cggcctgctg aatgcgcagg cggtcggcca 14280 tgccccaggc ttcgttttga catcggcgca ggtctttgta gtagtcttgc atgagccttt 14340 ctaccggcac ttcttcttct ccttcctctt gtcctgcatc tcttgcatct atcgctgcgg 14400 cggcggcgga gtttggccgt aggtggcgcc ctcttcctcc catgcgtgtg accccgaagc 14460 ccctcatcgg ctgaagcagg gctaggtcgg cgacaacgcg ctcggctaat atggcctgct 14520 gcacctgcgt gagggtagac tggaagtcat ccatgtccac aaagcggtgg tatgcgcccg 14580 tgttgatggt gtaagtgcag ttggccataa cggaccagtt aacggtctgg tgacccggct 14640 gcgagagctc ggtgtacctg agacgcgagt aagccctcga gtcaaatacg tagtcgttgc 14700 aagtccgcac caggtactgg tatcccacca aaaagtgcgg cggcggctgg cggtagaggg 14760 gccagcgtag ggtggccggg gctccggggg cgagatcttc caacataagg cgatgatatc 14820 cgtagatgta cctggacatc caggtgatgc cggcggcggt ggtggaggcg cgcggaaagt 14880 cgcggacgcg gttccagatg ttgcgcagcg gcaaaaagtg ctccatggtc gggacgctct 14940 ggccggtcag gcgcgcgcaa tcgttgacgc tctagcgtgc aaaaggagag cctgtaagcg 15000 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt 15060 tcgagccccg tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc 15120 caggtgtgcg acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg 15180 gctgctgcgc tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa 15240 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc 15300 gcgggacccc cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc 15360 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc 15420 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag 15480 caagagcagc ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg 15540 acatccgcgg ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg 15600 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag 15660 cggcacccaa gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac 15720 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca 15780 gggcgcgagc tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag 15840 cccgacgcgc gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta 15900 accgcatacg agcagacggt gaaccaggag attaactttc aaaaaagctt taacaaccac 15960 gtgcgtacgc ttgtggcgcg cgaggaggtg gctataggac tgatgcatct gtgggacttt 16020 gtaagcgcgc tggagcaaaa cccaaatagc aagccgctca tggcgcagct gttccttata 16080 gtgcagcaca gcagggacaa cgaggcattc agggatgcgc tgctaaacat agtagagccc 16140 gagggccgct ggctgctcga tttgataaac atcctgcaga gcatagtggt gcaggagcgc 16200 agcttgagcc tggctgacaa ggtggccgcc atcaactatt ccatgcttag cctgggcaag 16260 ttttacgccc gcaagatata ccatacccct tacgttccca tagacaagga ggtaaagatc 16320 gaggggttct acatgcgcat ggcgctgaag gtgcttacct tgagcgacga cctgggcgtt 16380 tatcgcaacg agcgcatcca caaggccgtg agcgtgagcc ggcggcgcga gctcagcgac 16440 cgcgagctga tgcacagcct gcaaagggcc ctggctggca cgggcagcgg cgatagagag 16500 gccgagtcct actttgacgc gggcgctgac ctgcgctggg ccccaagccg acgcgccctg 16560 gaggcagctg gggccggacc tgggctggcg gtggcacccg cgcgcgctgg caacgtcggc 16620 ggcgtggagg aatatgacga ggacgatgag tacgagccag aggacggcga gtactaagcg 16680 gtgatgtttc tgatcagatg atgcaagacg caacggaccc ggcggtgcgg gcggcgctgc 16740 agagccagcc gtccggcctt aactccacgg acgactggcg ccaggtcatg gaccgcatca 16800 tgtcgctgac tgcgcgcaat cctgacgcgt tccggcagca gccgcaggcc aaccggctct 16860 ccgcaattct ggaagcggtg gtcccggcgc gcgcaaaccc cacgcacgag aaggtgctgg 16920 cgatcgtaaa cgcgctggcc gaaaacaggg ccatccggcc cgacgaggcc ggcctggtct 16980 acgacgcgct gcttcagcgc gtggctcgtt acaacagcgg caacgtgcag accaacctgg 17040 accggctggt gggggatgtg cgcgaggccg tggcgcagcg tgagcgcgcg cagcagcagg 17100 gcaacctggg ctccatggtt gcactaaacg ccttcctgag tacacagccc gccaacgtgc 17160 cgcggggaca ggaggactac accaactttg tgagcgcact gcggctaatg gtgactgaga 17220 caccgcaaag tgaggtgtac cagtctgggc cagactattt tttccagacc agtagacaag 17280 gcctgcagac cgtaaacctg agccaggctt tcaaaaactt gcaggggctg tggggggtgc 17340 gggctcccac aggcgaccgc gcgaccgtgt ctagcttgct gacgcccaac tcgcgcctgt 17400 tgctgctgct aatagcgccc ttcacggaca gtggcagcgt gtcccgggac acatacctag 17460 gtcacttgct gacactgtac cgcgaggcca taggtcaggc gcatgtggac gagcatactt 17520 tccaggagat tacaagtgtc agccgcgcgc tggggcagga ggacacgggc agcctggagg 17580 caaccctaaa ctacctgctg accaaccggc ggcagaagat cccctcgttg cacagtttaa 17640 acagcgagga ggagcgcatt ttgcgctacg tgcagcagag cgtgagcctt aacctgatgc 17700 gcgacggggt aacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 17760 tgtatgcctc aaaccggccg tttatcaacc gcctaatgga ctacttgcat cgcgcggccg 17820 ccgtgaaccc cgagtatttc accaatgcca tcttgaaccc gcactggcta ccgccccctg 17880 gtttctacac cgggggattc gaggtgcccg agggtaacga tggattcctc tgggacgaca 17940 tagacgacag cgtgttttcc ccgcaaccgc agaccctgct agagttgcaa cagcgcgagc 18000 aggcagaggc ggcgctgcga aaggaaagct tccgcaggcc aagcagcttg tccgatctag 18060 gcgctgcggc cccgcggtca gatgctagta gcccatttcc aagcttgata gggtctctta 18120 ccagcactcg caccacccgc ccgcgcctgc tgggcgagga ggagtaccta aacaactcgc 18180 tgctgcagcc gcagcgcgaa aaaaacctgc ctccggcatt tcccaacaac gggatagaga 18240 gcctagtgga caagatgagt agatggaaga cgtacgcgca ggagcacagg gacgtgccag 18300 gcccgcgccc gcccacccgt cgtcaaaggc acgaccgtca gcggggtctg gtgtgggagg 18360 acgatgactc ggcagacgac agcagcgtcc tggatttggg agggagtggc aacccgtttg 18420 cgcaccttcg ccccaggctg gggagaatgt tttaaaaaaa aaaaagcatg atgcaaaata 18480 aaaaactcac caaggccatg gcaccgagcg ttggttttct tgtattcccc ttagtatgcg 18540 gcgcgcggcg atgtatgagg aaggtcctcc tccctcctac gagagtgtgg tgagcgcggc 18600 gccagtggcg gcggcgctgg gttctccctt cgatgctccc ctggacccgc cgtttgtgcc 18660 tccgcggtac ctgcggccta ccggggggag aaacagcatc cgttactctg agttggcacc 18720 cctattcgac accacccgtg tgtacctggt ggacaacaag tcaacggatg tggcatccct 18780 gaactaccag aacgaccaca gcaactttct gaccacggtc attcaaaaca atgactacag 18840 cccgggggag gcaagcacac agaccatcaa tcttgacgac cggtcgcact ggggcggcga 18900 cctgaaaacc atcctgcata ccaacatgcc aaatgtgaac gagttcatgt ttaccaataa 18960 gtttaaggcg cgggtgatgg tgtcgcgctt gcctactaag gacaatcagg tggagctgaa 19020 atacgagtgg gtggagttca cgctgcccga gggcaactac tccgagacca tgaccataga 19080 ccttatgaac aacgcgatcg tggagcacta cttgaaagtg ggcagacaga acggggttct 19140 ggaaagcgac atcggggtaa agtttgacac ccgcaacttc agactggggt ttgaccccgt 19200 cactggtctt gtcatgcctg gggtatatac aaacgaagcc ttccatccag acatcatttt 19260 gctgccagga tgcggggtgg acttcaccca cagccgcctg agcaacttgt tgggcatccg 19320 caagcggcaa cccttccagg agggctttag gatcacctac gatgatctgg agggtggtaa 19380 cattcccgca ctgttggatg tggacgccta ccaggcgagc ttgaaagatg acaccgaaca 19440 gggcgggggt ggcgcaggcg gcagcaacag cagtggcagc ggcgcggaag agaactccaa 19500 cgcggcagcc gcggcaatgc agccggtgga ggacatgaac gatcatgcca ttcgcggcga 19560 cacctttgcc acacgggctg aggagaagcg cgctgaggcc gaagcagcgg ccgaagctgc 19620 cgcccccgct gcgcaacccg aggtcgagaa gcctcagaag aaaccggtga tcaaacccct 19680 gacagaggac agcaagaaac gcagttacaa cctaataagc aatgacagca ccttcaccca 19740 gtaccgcagc tggtaccttg catacaacta cggcgaccct cagaccggaa tccgctcatg 19800 gaccctgctt tgcactcctg acgtaacctg cggctcggag caggtctact ggtcgttgcc 19860 agacatgatg caagaccccg tgaccttccg ctccacgcgc cagatcagca actttccggt 19920 ggtgggcgcc gagctgttgc ccgtgcactc caagagcttc tacaacgacc aggccgtcta 19980 ctcccaactc atccgccagt ttacctctct gacccacgtg ttcaatcgct ttcccgagaa 20040 ccagattttg gcgcgcccgc cagcccccac catcaccacc gtcagtgaaa acgttcctgc 20100 tctcacagat cacgggacgc taccgctgcg caacagcatc ggaggagtcc agcgagtgac 20160 cattactgac gccagacgcc gcacctgccc ctacgtttac aaggccctgg gcatagtctc 20220 gccgcgcgtc ctatcgagcc gcactttttg agcaagcatg tccatcctta tatcgcccag 20280 caataacaca ggctggggcc tgcgcttccc aagcaagatg tttggcgggg ccaagaagcg 20340 ctccgaccaa cacccagtgc gcgtgcgcgg gcactaccgc gcgccctggg gcgcgcacaa 20400 acgcggccgc actgggcgca ccaccgtcga tgacgccatc gacgcggtgg tggaggaggc 20460 gcgcaactac acgcccacgc cgccaccagt gtccacagtg gacgcggcca ttcagaccgt 20520 ggtgcgcgga gcccggcgct atgctaaaat gaagagacgg cggaggcgcg tagcacgtcg 20580 ccaccgccgc cgacccggca ctgccgccca acgcgcggcg gcggccctgc ttaaccgcgc 20640 acgtcgcacc ggccgacggg cggccatgcg ggccgctcga aggctggccg cgggtattgt 20700 cactgtgccc cccaggtcca ggcgacgagc ggccgccgca gcagccgcgg ccattagtgc 20760 tatgactcag ggtcgcaggg gcaacgtgta ttgggtgcgc gactcggtta gcggcctgcg 20820 cgtgcccgtg cgcacccgcc ccccgcgcaa ctagattgca agaaaaaact acttagactc 20880 gtactgttgt atgtatccag cggcggcggc gcgcaacgaa gctatgtcca agcgcaaaat 20940 caaagaagag atgctccagg tcatcgcgcc ggagatctat ggccccccga agaaggaaga 21000 gcaggattac aagccccgaa agctaaagcg ggtcaaaaag aaaaagaaag atgatgatga 21060 tgaacttgac gacgaggtgg aactgctgca cgctaccgcg cccaggcgac gggtacagtg 21120 gaaaggtcga cgcgtaaaac gtgttttgcg acccggcacc accgtagtct ttacgcccgg 21180 tgagcgctcc acccgcacct acaagcgcgt gtatgatgag gtgtacggcg acgaggacct 21240 gcttgagcag gccaacgagc gcctcgggga gtttgcctac ggaaagcggc ataaggacat 21300 gctggcgttg ccgctggacg agggcaaccc aacacctagc ctaaagcccg taacactgca 21360 gcaggtgctg cccgcgcttg caccgtccga agaaaagcgc ggcctaaagc gcgagtctgg 21420 tgacttggca cccaccgtgc agctgatggt acccaagcgc cagcgactgg aagatgtctt 21480 ggaaaaaatg accgtggaac ctgggctgga gcccgaggtc cgcgtgcggc caatcaagca 21540 ggtggcgccg ggactgggcg tgcagaccgt ggacgttcag atacccacta ccagtagcac 21600 cagtattgcc accgccacag agggcatgga gacacaaacg tccccggttg cctcagcggt 21660 ggcggatgcc gcggtgcagg cggtcgctgc ggccgcgtcc aagacctcta cggaggtgca 21720 aacggacccg tggatgtttc gcgtttcagc cccccggcgc ccgcgccgtt cgaggaagta 21780 cggcgccgcc agcgcgctac tgcccgaata tgccctacat ccttccattg cgcctacccc 21840 cggctatcgt ggctacacct accgccccag aagacgagca actacccgac gccgaaccac 21900 cactggaacc cgccgccgcc gtcgccgtcg ccagcccgtg ctggccccga tttccgtgcg 21960 cagggtggct cgcgaaggag gcaggaccct ggtgctgcca acagcgcgct accaccccag 22020 catcgtttaa aagccggtct ttgtggttct tgcagatatg gccctcacct gccgcctccg 22080 tttcccggtg ccgggattcc gaggaagaat gcaccgtagg aggggcatgg ccggccacgg 22140 cctgacgggc ggcatgcgtc gtgcgcacca ccggcggcgg cgcgcgtcgc accgtcgcat 22200 gcgcggcggt atcctgcccc tccttattcc actgatcgcc gcggcgattg gcgccgtgcc 22260 cggaattgca tccgtggcct tgcaggcgca gagacactga ttaaaaacaa gttgcatgtg 22320 gaaaaatcaa aataaaaagt ctggactctc acgctcgctt ggtcctgtaa ctattttgta 22380 gaatggaaga catcaacttt gcgtctctgg ccccgcgaca cggctcgcgc ccgttcatgg 22440 gaaactggca agatatcggc accagcaata tgagcggtgg cgccttcagc tggggctcgc 22500 tgtggagcgg cattaaaaat ttcggttcca ccgttaagaa ctatggcagc aaggcctgga 22560 acagcagcac aggccagatg ctgagggata agttgaaaga gcaaaatttc caacaaaagg 22620 tggtagatgg cctggcctct ggcattagcg gggtggtgga cctggccaac caggcagtgc 22680 aaaataagat taacagtaag cttgatcccc gccctcccgt agaggagcct ccaccggccg 22740 tggagacagt gtctccagag gggcgtggcg aaaagcgtcc gcgccccgac agggaagaaa 22800 ctctggtgac gcaaatagac gagcctccct cgtacgagga ggcactaaag caaggcctgc 22860 ccaccacccg tcccatcgcg cccatggcta ccggagtgct gggccagcac acacccgtaa 22920 cgctggacct gcctcccccc gccgacaccc agcagaaacc tgtgctgcca ggcccgaccg 22980 ccgttgttgt aacccgtcct agccgcgcgt ccctgcgccg cgccgccagc ggtccgcgat 23040 cgttgcggcc cgtagccagt ggcaactggc aaagcacact gaacagcatc gtgggtctgg 23100 gggtgcaatc cctgaagcgc cgacgatgct tctgatagct aacgtgtcgt atgtgtgtca 23160 tgtatgcgtc catgtcgccg ccagaggagc tgctgagccg ccgcgcgccc gctttccaag 23220 atggctaccc cttcgatgat gccgcagtgg tcttacatgc acatctcggg ccaggacgcc 23280 tcggagtacc tgagccccgg gctggtgcag tttgcccgcg ccaccgagac gtacttcagc 23340 ctgaataaca agtttagaaa ccccacggtg gcgcctacgc acgacgtgac cacagaccgg 23400 tcccagcgtt tgacgctgcg gttcatccct gtggaccgtg aggatactgc gtactcgtac 23460 aaggcgcggt tcaccctagc tgtgggtgat aaccgtgtgc tggacatggc ttccacgtac 23520 tttgacatcc gcggcgtgct ggacaggggc cctactttta agccctactc tggcactgcc 23580 tacaacgccc tggctcccaa gggtgcccca aatccttgcg aatgggatga agctgctact 23640 gctcttgaaa taaacctaga agaagaggac gatgacaacg aagacgaagt agacgagcaa 23700 gctgagcagc aaaaaactca cgtatttggg caggcgcctt attctggtat aaatattaca 23760 aaggagggta ttcaaatagg tgtcgaaggt caaacaccta aatatgccga taaaacattt 23820 caacctgaac ctcaaatagg agaatctcag tggtacgaaa cagaaattaa tcatgcagct 23880 gggagagtcc taaaaaagac taccccaatg aaaccatgtt acggttcata tgcaaaaccc 23940 acaaatgaaa atggagggca aggcattctt gtaaagcaac aaaatggaaa gctagaaagt 24000 caagtggaaa tgcaattttt ctcaactact gaggcagccg caggcaatgg tgataacttg 24060 actcctaaag tggtattgta cagtgaagat gtagatatag aaaccccaga cactcatatt 24120 tcttacatgc ccactattaa ggaaggtaac tcacgagaac taatgggcca acaatctatg 24180 cccaacaggc ctaattacat tgcttttagg gacaatttta ttggtctaat gtattacaac 24240 agcacgggta atatgggtgt tctggcgggc caagcatcgc agttgaatgc tgttgtagat 24300 ttgcaagaca gaaacacaga gctttcatac cagcttttgc ttgattccat tggtgataga 24360 accaggtact tttctatgtg gaatcaggct gttgacagct atgatccaga tgttagaatt 24420 attgaaaatc atggaactga agatgaactt ccaaattact gctttccact gggaggtgtg 24480 attaatacag agactcttac caaggtaaaa cctaaaacag gtcaggaaaa tggatgggaa 24540 aaagatgcta cagaattttc agataaaaat gaaataagag ttggaaataa ttttgccatg 24600 gaaatcaatc taaatgccaa cctgtggaga aatttcctgt actccaacat agcgctgtat 24660 ttgcccgaca agctaaagta cagtccttcc aacgtaaaaa tttctgataa cccaaacacc 24720 tacgactaca tgaacaagcg agtggtggct cccgggctag tggactgcta cattaacctt 24780 ggagcacgct ggtcccttga ctatatggac aacgtcaacc catttaacca ccaccgcaat 24840 gctggcctgc gctaccgctc aatgttgctg ggcaatggtc gctatgtgcc cttccacatc 24900 caggtgcctc agaagttctt tgccattaaa aacctccttc tcctgccggg ctcatacacc 24960 tacgagtgga acttcaggaa ggatgttaac atggttctgc agagctccct aggaaatgac 25020 ctaagggttg acggagccag cattaagttt gatagcattt gcctttacgc caccttcttc 25080 cccatggccc acaacaccgc ctccacgctt gaggccatgc ttagaaacga caccaacgac 25140 cagtccttta acgactatct ctccgccgcc aacatgctct accctatacc cgccaacgct 25200 accaacgtgc ccatatccat cccctcccgc aactgggcgg ctttccgcgg ctgggccttc 25260 acgcgcctta agactaagga aaccccatca ctgggctcgg gctacgaccc ttattacacc 25320 tactctggct ctatacccta cctagatgga accttttacc tcaaccacac ctttaagaag 25380 gtggccatta cctttgactc ttctgtcagc tggcctggca atgaccgcct gcttaccccc 25440 aacgagtttg aaattaagcg ctcagttgac ggggagggtt acaacgttgc ccagtgtaac 25500 atgaccaaag actggttcct ggtacaaatg ctagctaact ataacattgg ctaccagggc 25560 ttctatatcc cagagagcta caaggaccgc atgtactcct tctttagaaa cttccagccc 25620 atgagccgtc aggtggtgga tgatactaaa tacaaggact accaacaggt gggcatccta 25680 caccaacaca acaactctgg atttgttggc taccttgccc ccaccatgcg cgaaggacag 25740 gcctaccctg ctaacttccc ctatccgctt ataggcaaga ccgcagttga cagcattacc 25800 cagaaaaagt ttctttgcga tcgcaccctt tggcgcatcc cattctccag taactttatg 25860 tccatgggcg cactcacaga cctgggccaa aaccttctct acgccaactc cgcccacgcg 25920 ctagacatga cttttgaggt ggatcccatg gacgagccca cccttcttta tgttttgttt 25980 gaagtctttg acgtggtccg tgtgcaccag ccgcaccgcg gcgtcatcga aaccgtgtac 26040 ctgcgcacgc ccttctcggc cggcaacgcc acaacataaa gaagcaagca acatcaacaa 26100 cagctgccgc catgggctcc agtgagcagg aactgaaagc cattgtcaaa gatcttggtt 26160 gtgggccata ttttttgggc acctatgaca agcgctttcc aggctttgtt tctccacaca 26220 agctcgcctg cgccatagtc aatacggccg gtcgcgagac tgggggcgta cactggatgg 26280 cctttgcctg gaacccgcac tcaaaaacat gctacctctt tgagcccttt ggcttttctg 26340 accagcgact caagcaggtt taccagtttg agtacgagtc actcctgcgc cgtagcgcca 26400 ttgcttcttc ccccgaccgc tgtataacgc tggaaaagtc cacccaaagc gtacaggggc 26460 ccaactcggc cgcctgtgga ctattctgct gcatgtttct ccacgccttt gccaactggc 26520 cccaaactcc catggatcac aaccccacca tgaaccttat taccggggta cccaactcca 26580 tgctcaacag tccccaggta cagcccaccc tgcgtcgcaa ccaggaacag ctctacagct 26640 tcctggagcg ccactcgccc tacttccgca gccacagtgc gcagattagg agcgccactt 26700 ctttttgtca cttgaaaaac atgtaaaaat aatgtactag agacactttc aataaaggca 26760 aatgctttta tttgtacact ctcgggtgat tatttacccc cacccttgcc gtctgcgccg 26820 tttaaaaatc aaaggggttc tgccgcgcat cgctatgcgc cactggcagg gacacgttgc 26880 gatactggtg tttagtgctc cacttaaact caggcacaac catccgcggc agctcggtga 26940 agttttcact ccacaggctg cgcaccatca ccaacgcgtt tagcaggtcg ggcgccgata 27000 tcttgaagtc gcagttgggg cctccgccct gcgcgcgcga gttgcgatac acagggttgc 27060 agcactggaa cactatcagc gccgggtggt gcacgctggc cagcacgctc ttgtcggaga 27120 tcagatccgc gtccaggtcc tccgcgttgc tcagggcgaa cggagtcaac tttggtagct 27180 gccttcccaa aaagggcgcg tgcccaggct ttgagttgca ctcgcaccgt agtggcatca 27240 aaaggtgacc gtgcccggtc tgggcgttag gatacagcgc ctgcataaaa gccttgatct 27300 gcttaaaagc cacctgagcc tttgcgcctt cagagaagaa catgccgcaa gacttgccgg 27360 aaaactgatt ggccggacag gccgcgtcgt gcacgcagca ccttgcgtcg gtgttggaga 27420 tctgcaccac atttcggccc caccggttct tcacgatctt ggccttgcta gactgctcct 27480 tcagcgcgcg ctgcccgttt tcgctcgtca catccatttc aatcacgtgc tccttattta 27540 tcataatgct tccgtgtaga cacttaagct cgccttcgat ctcagcgcag cggtgcagcc 27600 acaacgcgca gcccgtgggc tcgtgatgct tgtaggtcac ctctgcaaac gactgcaggt 27660 acgcctgcag gaatcgcccc atcatcgtca caaaggtctt gttgctggtg aaggtcagct 27720 gcaacccgcg gtgctcctcg ttcagccagg tcttgcatac ggccgccaga gcttccactt 27780 ggtcaggcag tagtttgaag ttcgccttta gatcgttatc cacgtggtac ttgtccatca 27840 gcgcgcgcgc agcctccatg cccttctccc acgcagacac gatcggcaca ctcagcgggt 27900 tcatcaccgt aatttcactt tccgcttcgc tgggctcttc ctcttcctct tgcgtccgca 27960 taccacgcgc cactgggtcg tcttcattca gccgccgcac tgtgcgctta cctcctttgc 28020 catgcttgat tagcaccggt gggttgctga aacccaccat ttgtagcgcc acatcttctc 28080 tttcttcctc gctgtccacg attacctctg gtgatggcgg gcgctcgggc ttgggagaag 28140 ggcgcttctt tttcttcttg ggcgcaatgg ccaaatccgc cgccgaggtc gatggccgcg 28200 ggctgggtgt gcgcggcacc agcgcgtctt gtgatgagtc ttcctcgtcc tcggactcga 28260 tacgccgcct catccgcttt tttgggggcg cccggggagg cggcggcgac ggggacgggg 28320 acgacacgtc ctccatggtt gggggacgtc gcgccgcacc gcgtccgcgc tcgggggtgg 28380 tttcgcgctg ctcctcttcc cgactggcca tttccttctc ctataggcag aaaaagatca 28440 tggagtcagt cgagaagaag gacagcctaa ccgccccctc tgagttcgcc accaccgcct 28500 ccaccgatgc cgccaacgcg cctaccacct tccccgtcga ggcacccccg cttgaggagg 28560 aggaagtgat tatcgagcag gacccaggtt ttgtaagcga agacgacgag gaccgctcag 28620 taccaacaga ggataaaaag caagaccagg acaacgcaga ggcaaacgag gaacaagtcg 28680 ggcgggggga cgaaaggcat ggcgactacc tagatgtggg agacgacgtg ctgttgaagc 28740 atctgcagcg ccagtgcgcc attatctgcg acgcgttgca agagcgcagc gatgtgcccc 28800 tcgccatagc ggatgtcagc cttgcctacg aacgccacct attctcaccg cgcgtacccc 28860 ccaaacgcca agaaaacggc acatgcgagc ccaacccgcg cctcaacttc taccccgtat 28920 ttgccgtgcc agaggtgctt gccacctatc acatcttttt ccaaaactgc aagatacccc 28980 tatcctgccg tgccaaccgc agccgagcgg acaagcagct ggccttgcgg cagggcgctg 29040 tcatacctga tatcgcctcg ctcaacgaag tgccaaaaat ctttgagggt cttggacgcg 29100 acgagaagcg cgcggcaaac gctctgcaac aggaaaacag cgaaaatgaa agtcactctg 29160 gagtgttggt ggaactcgag ggtgacaacg cgcgcctagc cgtactaaaa cgcagcatcg 29220 aggtcaccca ctttgcctac ccggcactta acctaccccc caaggtcatg agcacagtca 29280 tgagtgagct gatcgtgcgc cgtgcgcagc ccctggagag ggatgcaaat ttgcaagaac 29340 aaacagagga gggcctaccc gcagttggcg acgagcagct agcgcgctgg cttcaaacgc 29400 gcgagcctgc cgacttggag gagcgacgca aactaatgat ggccgcagtg ctcgttaccg 29460 tggagcttga gtgcatgcag cggttctttg ctgacccgga gatgcagcgc aagctagagg 29520 aaacattgca ctacaccttt cgacagggct acgtacgcca ggcctgcaag atctccaacg 29580 tggagctctg caacctggtc tcctaccttg gaattttgca cgaaaaccgc cttgggcaaa 29640 acgtgcttca ttccacgctc aagggcgagg cgcgccgcga ctacgtccgc gactgcgttt 29700 acttatttct atgctacacc tggcagacgg ccatgggcgt ttggcagcag tgcttggagg 29760 agtgcaacct caaggagctg cagaaactgc taaagcaaaa cttgaaggac ctatggacgg 29820 ccttcaacga gcgctccgtg gccgcgcacc tggcggacat cattttcccc gaacgcctgc 29880 ttaaaaccct gcaacagggt ctgccagact tcaccagtca aagcatgttg cagaacttta 29940 ggaactttat cctagagcgc tcaggaatct tgcccgccac ctgctgtgca cttcctagcg 30000 actttgtgcc cattaagtac cgcgaatgcc ctccgccgct ttggggccac tgctaccttc 30060 tgcagctagc caactacctt gcctaccact ctgacataat ggaagacgtg agcggtgacg 30120 gtctactgga gtgtcactgt cgctgcaacc tatgcacccc gcaccgctcc ctggtttgca 30180 attcgcagct gcttaacgaa agtcaaatta tcggtacctt tgagctgcag ggtccctcgc 30240 ctgacgaaaa gtccgcggct ccggggttga aactcactcc ggggctgtgg acgtcggctt 30300 accttcgcaa atttgtacct gaggactacc acgcccacga gattaggttc tacgaagacc 30360 aatcccgccc gcctaatgcg gagcttaccg cctgcgtcat tacccagggc cacattcttg 30420 gccaattgca agccatcaac aaagcccgcc aagagtttct gctacgaaag ggacgggggg 30480 tttacttgga cccccagtcc ggcgaggagc tcaacccaat ccccccgccg ccgcagccct 30540 atcagcagca gccgcgggcc cttgcttccc aggatggcac ccaaaaagaa gctgcagctg 30600 ccgccgccac ccacggacga ggaggaatac tgggacagtc aggcagagga ggttttggac 30660 gaggaggagg aggacatgat ggaagactgg gagagcctag acgaggaagc ttccgaggtc 30720 gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat tcccctcgcc ggcgccccag 30780 aaatcggcaa ccggttccag catggctaca acctccgctc ctcaggcgcc gccggcactg 30840 cccgttcgcc gacccaaccg tagatgggac accactggaa ccagggccgg taagtccaag 30900 cagccgccgc cgttagccca agagcaacaa cagcgccaag gctaccgctc atggcgcggg 30960 cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg gcaacatctc cttcgcccgc 31020 cgctttcttc tctaccatca cggcgtggcc ttcccccgta acatcctgca ttactaccgt 31080 catctctaca gcccatactg caccggcggc agcggcagca acagcagcgg ccacacagaa 31140 gcaaaggcga ccggatagca agactctgac aaagcccaag aaatccacag cggcggcagc 31200 agcaggagga ggagcgctgc gtctggcgcc caacgaaccc gtatcgaccc gcgagcttag 31260 aaacaggatt tttcccactc tgtatgctat atttcaacag agcaggggcc aagaacaaga 31320 gctgaaaata aaaaacaggt ctctgcgatc cctcacccgc agctgcctgt atcacaaaag 31380 cgaagatcag cttcggcgca cgctggaaga cgcggaggct ctcttcagta aatactgcgc 31440 gctgactctt aaggactagt ttcgcgccct ttctcaaatt taagcgcgaa aactacgtca 31500 tctccagcgg ccacacccgg cgccagcacc tgttgtcagc gccattatga gcaaggaaat 31560 tcccacgccc tacatgtgga gttaccagcc acaaatggga cttgcggctg gagctgccca 31620 agactactca acccgaataa actacatgag cgcgggaccc cacatgatat cccgggtcaa 31680 cggaatacgc gcccaccgaa accgaattct cctggaacag gcggctatta ccaccacacc 31740 tcgtaataac cttaatcccc gtagttggcc cgctgccctg gtgtaccagg aaagtcccgc 31800 tcccaccact gtggtacttc ccagagacgc ccaggccgaa gttcagatga ctaactcagg 31860 ggcgcagctt gcgggcggct ttcgtcacag ggtgcggtcg cccgggcagg gtataactca 31920 cctgacaatc agagggcgag gtattcagct caacgacgag tcggtgagct cctcgcttgg 31980 tctccgtccg gacgggacat ttcagatcgg cggcgccggc cgctcttcat tcacgcctcg 32040 tcaggcaatc ctaactctgc agacctcgtc ctctgagccg cgctctggag gcattggaac 32100 tctgcaattt attgaggagt ttgtgccatc ggtctacttt aaccccttct cgggacctcc 32160 cggccactat ccggatcaat ttattcctaa ctttgacgcg gtaaaggact cggcggacgg 32220 ctacgactga atgttaagtg gagaggcaga gcaactgcgc ctgaaacacc tggtccactg 32280 tcgccgccac aagtgctttg cccgcgactc cggtgagttt tgctactttg aattgcccga 32340 ggatcatatc gagggcccgg cgcacggcgt ccggcttacc gcccagggag agcttgcccg 32400 tagcctgatt cgggagttta cccagcgccc cctgctagtt gagcgggaca ggggaccctg 32460 tgttctcact gtgatttgca actgtcctaa ccctggatta catcaagatc ctctagttaa 32520 tgtcaggtcg cctaagtcga ttaactagag tacccgggga tcttattccc tttaactaat 32580 aaaaaaaaat aataaagcat cacttactta aaatcagtta gcaaatttct gtccagttta 32640 ttcagcagca cctccttgcc ctcctcccag ctctggtatt gcagcttcct cctggctgca 32700 aactttctcc acaatctaaa tggaatgtca gtttcctcct gttcctgtcc atccgcaccc 32760 actatcttca tgttgttgca gatgaagcgc gcaagaccgt ctgaagatac cttcaacccc 32820 gtgtatccat atgacacgga aaccggtcct ccaactgtgc cttttcttac tcctcccttt 32880 gtatccccca atgggtttca agagagtccc cctggggtac tctctttgcg cctatccgaa 32940 cctctagtta cctccaatgg catgcttgcg ctcaaaatgg gcaacggcct ctctctggac 33000 gaggccggca accttacctc ccaaaatgta accactgtga gcccacctct caaaaaaacc 33060 aagtcaaaca taaacctgga aatatctgca cccctcacag ttacctcaga agccctaact 33120 gtggctgccg ccgcacctct aatggtcgcg ggcaacacac tcaccatgca atcacaggcc 33180 ccgctaaccg tgcacgactc caaacttagc attgccaccc aaggacccct cacagtgtca 33240 gaaggaaagc tagccctgca aacatcaggc cccctcacca ccaccgatag cagtaccctt 33300 actatcactg cctcaccccc tctaactact gccactggta gcttgggcat tgacttgaaa 33360 gagcccattt atacacaaaa tggaaaacta ggactaaagt acggggctcc tttgcatgta 33420 acagacgacc taaacacttt gaccgtagca actggtccag gtgtgactat taataatact 33480 tccttgcaaa ctaaagttac tggagccttg ggttttgatt cacaaggcaa tatgcaactt 33540 aatgtagcag gaggactaag gattgattct caaaacagac gccttatact tgatgttagt 33600 tatccgtttg atgctcaaaa ccaactaaat ctaagactag gacagggccc tctttttata 33660 aactcagccc acaacttgga tattaactac aacaaaggcc tttacttgtt tacagcttca 33720 aacaattcca aaaagcttga ggttaaccta agcactgcca aggggttgat gtttgacgct 33780 acagccatag ccattaatgc aggagatggg cttgaatttg gttcacctaa tgcaccaaac 33840 acaaatcccc tcaaaacaaa aattggccat ggcctagaat ttgattcaaa caaggctatg 33900 gttcctaaac taggaactgg ccttagtttt gacagcacag gtgccattac agtaggaaac 33960 aaaaataatg ataagctaac tttgtggacc ggaataaacc ctccacctaa ctgtcaaatt 34020 gtggaaaaca ctaatacaaa tgatggcaaa cttactttag tattagtaaa aaatggaggg 34080 cttgttaatg gctacgtgtc tctagttggt gtatcagaca ctgtgaacca aatgttcaca 34140 caaaagacag caaacatcca attaagatta tattttgact cttctggaaa tctattaact 34200 gaggaatcag acttaaaaat tccacttaaa aataaatctt ctacagcgac cagtgaaact 34260 gtagccagca gcaaagcctt tatgccaagt actacagctt atcccttcaa caccactact 34320 agggatagtg aaaactacat tcatggaata tgttactaca tgactagtta tgatagaagt 34380 ctatttccct tgaacatttc tataatgcta aacagccgta tgatttcttc caatgttgcc 34440 tatgccatac aatttgaatg gaatctaaat gcaagtgaat ctccagaaag caacatagct 34500 acgctgacca catccccctt tttcttttct tacattacag aagacgacaa ctaaagaatc 34560 gtttgtgtta tgtttcaacg tgtttatttt tcaattgcag aaaatttcaa gtcatttttc 34620 attcagtagt atagccccac caccacatag cttatacaga tcaccgtacc ttaatcaaac 34680 tcacagaacc ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct 34740 ttctccccgg ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt 34800 tatattccac acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc 34860 gggcagctca cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac 34920 ttgcggttgc ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata 34980 atcgtgcatc aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg 35040 ccgctccgtc ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc 35100 ccgcagcata aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc 35160 agcacagtaa ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct 35220 gtatccaaag ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag 35280 gtagattaag tggcgacccc tcataaacac gctggacata aacattacct cttttggcat 35340 gttgtaattc accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac 35400 caccatccta aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg 35460 actggaacaa tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat 35520 gatatcaatg ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc 35580 ctcccgcgtt agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac 35640 actgcaggga agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg 35700 cagcagcgga tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg 35760 atccctactg tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc 35820 aaatggaacg ccggacgtag tcatatttcc tgaagcaaaa ccaggtgcgg gcgtgacaaa 35880 cagatctgcg tctccggtct cgccgcttag atcgctctgt gtagtagttg tagtatatcc 35940 actctctcaa agcatccagg cgccccctgg cttcgggttc tatgtaaact ccttcatgcg 36000 ccgctgccct gataacatcc accaccgcag aataagccac acccagccaa cctacacatt 36060 cgttctgcga gtcacacacg ggaggagcgg gaagagctgg aagaaccatg tttttttttt 36120 tattccaaaa gattatccaa aacctcaaaa tgaagatcta ttaagtgaac gcgctcccct 36180 ccggtggcgt ggtcaaactc tacagccaaa gaacagataa tggcatttgt aagatgttgc 36240 acaatggctt ccaaaaggca aacggccctc acgtccaagt ggacgtaaag gctaaaccct 36300 tcagggtgaa tctcctctat aaacattcca gcaccttcaa ccatgcccaa ataattctca 36360 tctcgccacc ttctcaatat atctctaagc aaatcccgaa tattaagtcc ggccattgta 36420 aaaatctgct ccagagcgcc ctccaccttc agcctcaagc agcgaatcat gattgcaaaa 36480 attcaggttc ctcacagacc tgtataagat tcaaaagcgg aacattaaca aaaataccgc 36540 gatcccgtag gtcccttcgc agggccagct gaacataatc gtgcaggtct gcacggacca 36600 gcgcggccac ttccccgcca ggaaccatga caaaagaacc cacactgatt atgacacgca 36660 tactcggagc tatgctaacc agcgtagccc cgatgtaagc ttgttgcatg ggcggcgata 36720 taaaatgcaa ggtgctgctc aaaaaatcag gcaaagcctc gcgcaaaaaa gaaagcacat 36780 cgtagtcatg ctcatgcaga taaaggcagg taagctccgg aaccaccaca gaaaaagaca 36840 ccatttttct ctcaaacatg tctgcgggtt tctgcataaa cacaaaataa aataacaaaa 36900 aaacatttaa acattagaag cctgtcttac aacaggaaaa acaaccctta taagcataag 36960 acggactacg gccatgccgg cgtgaccgta aaaaaactgg tcaccgtgat taaaaagcac 37020 caccgacagc tcctcggtca tgtccggagt cataatgtaa gactcggtaa acacatcagg 37080 ttgattcaca tcggtcagtg ctaaaaagcg accgaaatag cccgggggaa tacatacccg 37140 caggcgtaga gacaacatta cagcccccat aggaggtata acaaaattaa taggagagaa 37200 aaacacataa acacctgaaa aaccctcctg cctaggcaaa atagcaccct cccgctccag 37260 aacaacatac agcgcttcca cagcggcagc cataacagtc agccttacca gtaaaaaaga 37320 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa 37380 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca 37440 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc 37500 acaacttcct caaatcgtca cttccgtttt cccacgttac gtcacttccc attttaagaa 37560 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt 37620 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc 37680 aaaataaggt atattattga tgatg 37705 <210> 29 <211> 34766 <212> DNA <213> Artificial Sequence <220> <223> #4 vector <400> 29 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct 960 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 1020 agacaccggg accgatccag cctccgattt gccaccatgt ttgtgttcct ggtgctgctg 1080 ccactggtgt ccagccagtg tgtgaacctg accaccagga cccaacttcc tcctgcctac 1140 accaactcct tcaccagggg agtctactac cctgacaagg tgttcaggtc ctctgtgctg 1200 cacagcaccc aggacctgtt cctgccattc ttcagcaatg tgacctggtt ccatgccatc 1260 catgtgtctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc attcaatgat 1320 ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat ttttggcacc 1380 accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa tgtggtgatt 1440 aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg gagtctacta ccacaagaac 1500 aacaagtcct ggatggagtc tgagttcagg gtctactcct ctgccaacaa ctgtaccttt 1560 gaatatgtga gccaaccatt cctgatggac ttggagggca agcagggcaa cttcaagaac 1620 ctgagggagt ttgtgttcaa gaacattgat ggctacttca agatttacag caaacacaca 1680 ccaatcaacc tggtgaggga cctgccacag ggcttctctg ccttggaacc actggtggac 1740 ctgccaattg gcatcaacat caccaggttc cagaccctgc tggctctgca caggtcctac 1800 ctgacacctg gagactcctc ctctggctgg acagcaggag cagcagccta ctatgtgggc 1860 tacctccaac caaggacctt cctgctgaaa tacaatgaga atggcaccat cacagatgct 1920 gtggactgtg ccctggaccc actgtctgag accaagtgta ccctgaaatc cttcacagtg 1980 gagaagggca tctaccagac cagcaacttc agggtccaac caacagagag cattgtgagg 2040 tttccaaaca tcaccaacct gtgtccattt ggagaggtgt tcaatgccac caggtttgcc 2100 tctgtctatg cctggaacag gaagaggatt agcaactgtg tggctgacta ctctgtgctc 2160 tacaactctg cctccttcag caccttcaag tgttatggag tgagcccaac caaactgaat 2220 gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga ttaggggaga tgaggtgaga 2280 cagattgccc ctggacaaac aggcaagatt gctgactaca actacaaact gcctgatgac 2340 ttcacaggct gtgtgattgc ctggaacagc aacaacctgg acagcaaggt gggaggcaac 2400 tacaactacc tctacagact gttcaggaag agcaacctga aaccatttga gagggacatc 2460 agcacagaga tttaccaggc tggcagcaca ccatgtaatg gagtggaggg cttcaactgt 2520 tactttccac tccaatccta tggcttccaa ccaaccaatg gagtgggcta ccaaccatac 2580 agggtggtgg tgctgtcctt tgaactgctc catgcccctg ccacagtgtg tggaccaaag 2640 aagagcacca acctggtgaa gaacaagtgt gtgaacttca acttcaatgg actgacaggc 2700 acaggagtgc tgacagagag caacaagaag ttcctgccat tccaacagtt tggcagggac 2760 attgctgaca ccacagatgc tgtgagggac ccacagacct tggagattct ggacatcaca 2820 ccatgttcct ttggaggagt gtctgtgatt acacctggca ccaacaccag caaccaggtg 2880 gctgtgctct accaggatgt gaactgtact gaggtgcctg tggctatcca tgctgaccaa 2940 cttacaccaa cctggagggt ctacagcaca ggcagcaatg tgttccagac cagggctggc 3000 tgtctgattg gagcagagca tgtgaacaac tcctatgagt gtgacatccc aattggagca 3060 ggcatctgtg cctcctacca gacccagacc ggtggcggtg ggtcgggcgg tggtgggtcg 3120 ggtggcggcg gttccaggtc tgtggcaagc cagagcatca ttgcctacac aatgagtctg 3180 ggagcagaga actctgtggc ttacagcaac aacagcattg ccatcccaac caacttcacc 3240 atctctgtga ccacagagat tctgcctgtg agtatgacca agacctctgt ggactgtaca 3300 atgtatatct gtggagacag cacagagtgt agcaacctgc tgctccaata tggctccttc 3360 tgtacccaac ttaacagggc tctgacaggc attgctgtgg aacaggacaa gaacacccag 3420 gaggtgtttg cccaggtgaa gcagatttac aagacacctc caatcaagga ctttggaggc 3480 ttcaacttca gccagattct gcctgaccca agcaagccaa gcaagaggtc cttcattgag 3540 gacctgctgt tcaacaaggt gaccctggct gatgctggct tcatcaagca atatggagac 3600 tgtctgggag acattgctgc cagggacctg atttgtgccc agaagttcaa tggactgaca 3660 gtgctgcctc cactgctgac agatgagatg attgcccaat acacctctgc cctgctggct 3720 ggcaccatca cctctggctg gacctttgga gcaggagcag ccctccaaat cccatttgct 3780 atgcagatgg cttacaggtt caatggcatt ggagtgaccc agaatgtgct ctatgagaac 3840 cagaaactga ttgccaacca gttcaactct gccattggca agattcagga ctccctgtcc 3900 agcacagcct ctgccctggg caaactccaa gatgtggtga accagaatgc ccaggctctg 3960 aacaccctgg tgaagcaact ttccagcaac tttggagcca tctcctctgt gctgaatgac 4020 atcctgagca gactggacaa ggtggaggct gaggtccaga ttgacagact gattacaggc 4080 agactccaat ccctccaaac ctatgtgacc caacaactta tcagggctgc tgagattagg 4140 gcatctgcca acctggctgc caccaagatg agtgagtgtg tgctgggaca aagcaagagg 4200 gtggacttct gtggcaaggg ctaccacctg atgagttttc cacagtctgc ccctcatgga 4260 gtggtgttcc tgcatgtgac ctatgtgcct gcccaggaga agaacttcac cacagcccct 4320 gccatctgcc atgatggcaa ggctcacttt ccaagggagg gagtgtttgt gagcaatggc 4380 acccactggt ttgtgaccca gaggaacttc tatgaaccac agattatcac cacagacaac 4440 acctttgtgt ctggcaactg tgatgtggtg attggcattg tgaacaacac agtctatgac 4500 ccactccaac ctgaactgga ctccttcaag gaggaactgg acaaatactt caagaaccac 4560 accagccctg atgtggacct gggagacatc tctggcatca atgcctctgt ggtgaacatc 4620 cagaaggaga ttgacagact gaatgaggtg gctaagaacc tgaatgagtc cctgattgac 4680 ctccaagaac tgggcaaata tgaacaatac atcaagtggc catgaaaatt gatcataatc 4740 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 4800 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 4860 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 4920 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcggatctgg gcgtggttaa 4980 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 5040 ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa 5100 cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc 5160 gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt 5220 tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga 5280 ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg 5340 atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg 5400 tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc 5460 ccaatgcggt ttaaaacata aataaaaaac cagactctgt ttggatttgg atcaagcaag 5520 tgtcttgctg tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc 5580 ggtcgttgag ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca 5640 gatacatggg cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct 5700 gcggggtggt gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa 5760 tgtctttcag tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc 5820 ggttaagctg ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta 5880 ggttggctat gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca 5940 cagtgtatcc ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga 6000 acttggagac gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa 6060 tgggcccacg ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt 6120 gttccaggat gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact 6180 gcggtataat ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc 6240 acgctttgag ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt 6300 ccggggtagg ggagatcagc tgggaagaaa gcaggttcct gagcagctgc gacttaccgc 6360 agccggtggg cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc 6420 agctgccgtc atccctgagc aggggggcca cttcgttaag catgtccctg actcgcatgt 6480 tttccctgac caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg 6540 aagcaaagtt tttcaacggt ttgagaccgt ccgccgtagg catgcttttg agcgtttgac 6600 caagcagttc caggcggtcc cacagctcgg tcacctgctc tacggcatct cgatccagca 6660 tatctcctcg tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc 6720 cagacgggcc agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt 6780 cacggtgaag gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga ggctggtcct 6840 gctggtgctg aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat 6900 ggtgtcatag tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga 6960 ggcgccgcac gaggggcagt gcagactttt gagggcgtag agcttgggcg cgagaaatac 7020 cgattccggg gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag 7080 ccaggtgagc tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg 7140 tttcttacct ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt 7200 gtccccgtat acagacttga gaggcctgtc ctcgagcggt gttccgcggt cctcctcgta 7260 tagaaactcg gaccactctg agacaaaggc tcgcgtccag gccagcacga aggaggctaa 7320 gtgggagggg tagcggtcgt tgtccactag ggggtccact cgctccaggg tgtgaagaca 7380 catgtcgccc tcttcggcat caaggaaggt gattggtttg taggtgtagg ccacgtgacc 7440 gggtgttcct gaaggggggc tataaaaggg ggtgggggcg cgttcgtcct cactctcttc 7500 cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctgaa aagcgggcat 7560 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc 7620 cgcggtgatg cctttgaggg tggccgcatc catctggtca gaaaagacaa tctttttgtt 7680 gtcaagcttg gtggcaaacg acccgtagag ggcgttggac agcaacttgg cgatggagcg 7740 cagggtttgg tttttgtcgc gatcggcgcg ctccttggcc gcgatgttta gctgcacgta 7800 ttcgcgcgca acgcaccgcc attcgggaaa gacggtggtg cgctcgtcgg gcaccaggtg 7860 cacgcgccaa ccgcggttgt gcagggtgac aaggtcaacg ctggtggcta cctctccgcg 7920 taggcgctcg ttggtccagc agaggcggcc gcccttgcgc gagcagaatg gcggtagggg 7980 gtctagctgc gtctcgtccg gggggtctgc gtccacggta aagaccccgg gcagcaggcg 8040 cgcgtcgaag tagtctatct tgcatccttg caagtctagc gcctgctgcc atgcgcgggc 8100 ggcaagcgcg cgctcgtatg ggttgagtgg gggaccccat ggcatggggt gggtgagcgc 8160 ggaggcgtac atgccgcaaa tgtcgtaaac gtagaggggc tctctgagta ttccaagata 8220 tgtagggtag catcttccac cgcggatgct ggcgcgcacg taatcgtata gttcgtgcga 8280 gggagcgagg aggtcgggac cgaggttgct acgggcgggc tgctctgctc ggaagactat 8340 ctgcctgaag atggcatgtg agttggatga tatggttgga cgctggaaga cgttgaagct 8400 ggcgtctgtg agacctaccg cgtcacgcac gaaggaggcg taggagtcgc gcagcttgtt 8460 gaccagctcg gcggtgacct gcacgtctag ggcgcagtag tccagggttt ccttgatgat 8520 gtcatactta tcctgtccct tttttttcca cagctcgcgg ttgaggacaa actcttcgcg 8580 gtctttccag tactcttgga tcggaaaccc gtcggcctcc gaacggtaag agcctagcat 8640 gtagaactgg ttgacggcct ggtaggcgca gcatcccttt tctacgggta gcgcgtatgc 8700 ctgcgcggcc ttccggagcg aggtgtgggt gagcgcaaag gtgtccctga ccatgacttt 8760 gaggtactgg tatttgaagt cagtgtcgtc gcatccgccc tgctcccaga gcaaaaagtc 8820 cgtgcgcttt ttggaacgcg gatttggcag ggcgaaggtg acatcgttga agagtatctt 8880 tcccgcgcga ggcataaagt tgcgtgtgat gcggaagggt cccggcacct cggaacggtt 8940 gttaattacc tgggcggcga gcacgatctc gtcaaagccg ttgatgttgt ggcccacaat 9000 gtaaagttcc aagaagcgcg ggatgccctt gatggaaggc aattttttaa gttcctcgta 9060 ggtgagctct tcaggggagc tgagcccgtg ctctgaaagg gcccagtctg caagatgagg 9120 gttggaagcg acgaatgagc tccacaggtc acgggccatt agcatttgca ggtggtcgcg 9180 aaaggtccta aactggcgac ctatggccat tttttctggg gtgatgcagt agaaggtaag 9240 cgggtcttgt tcccagcggt cccatccaag gttcgcggct aggtctcgcg cggcagtcac 9300 tagaggctca tctccgccga acttcatgac cagcatgaag ggcacgagct gcttcccaaa 9360 ggcccccatc caagtatagg tctctacatc gtaggtgaca aagagacgct cggtgcgagg 9420 atgcgagccg atcgggaaga actggatctc ccgccaccaa ttggaggagt ggctattgat 9480 gtggtgaaag tagaagtccc tgcgacgggc cgaacactcg tgctggcttt tgtaaaaacg 9540 tgcgcagtac tggcagcggt gcacgggctg tacatcctgc acgaggttga cctgacgacc 9600 gcgcacaagg aagcagagtg ggaatttgag cccctcgcct ggcgggtttg gctggtggtc 9660 ttctacttcg gctgcttgtc cttgaccgtc tggctgctcg aggggagtta cggtggatcg 9720 gaccaccacg ccgcgcgagc ccaaagtcca gatgtccgcg cgcggcggtc ggagcttgat 9780 gacaacatcg cgcagatggg agctgtccat ggtctggagc tcccgcggcg tcaggtcagg 9840 cgggagctcc tgcaggttta cctcgcatag acgggtcagg gcgcgggcta gatccaggtg 9900 atacctaatt tccaggggct ggttggtggc ggcgtcgatg gcttgcaaga ggccgcatcc 9960 ccgcggcgcg actacggtac cgcgcggcgg gcggtgggcc gcgggggtgt ccttggatga 10020 tgcatctaaa agcggtgacg cgggcgagcc cccggaggta gggggggctc cggacccgcc 10080 gggagagggg gcaggggcac gtcggcgccg cgcgcgggca ggagctggtg ctgcgcgcgt 10140 aggttgctgg cgaacgcgac gacgcggcgg ttgatctcct gaatctggcg cctctgcgtg 10200 aagacgacgg gcccggtgag cttgaacctg aaagagagtt cgacagaatc aatttcggtg 10260 tcgttgacgg cggcctggcg caaaatctcc tgcacgtctc ctgagttgtc ttgataggcg 10320 atctcggcca tgaactgctc gatctcttcc tcctggagat ctccgcgtcc ggctcgctcc 10380 acggtggcgg cgaggtcgtt ggaaatgcgg gccatgagct gcgagaaggc gttgaggcct 10440 ccctcgttcc agacgcggct gtagaccacg cccccttcgg catcgcgggc gcgcatgacc 10500 acctgcgcga gattgagctc cacgtgccgg gcgaagacgg cgtagtttcg caggcgctga 10560 aagaggtagt tgagggtggt ggcggtgtgt tctgccacga agaagtacat aacccagcgt 10620 cgcaacgtgg attcgttgat atcccccaag gcctcaaggc gctccatggc ctcgtagaag 10680 tccacggcga agttgaaaaa ctgggagttg cgcgccgaca cggttaactc ctcctccaga 10740 agacggatga gctcggcgac agtgtcgcgc acctcgcgct caaaggctac aggggcctct 10800 tcttcttctt caatctcctc ttccataagg gcctcccctt cttcttcttc tggcggcggt 10860 gggggagggg ggacacggcg gcgacgacgg cgcaccggga ggcggtcgac aaagcgctcg 10920 atcatctccc cgcggcgacg gcgcatggtc tcggtgacgg cgcggccgtt ctcgcggggg 10980 cgcagttgga agacgccgcc cgtcatgtcc cggttatggg ttggcggggg gctgccatgc 11040 ggcagggata cggcgctaac gatgcatctc aacaattgtt gtgtaggtac tccgccgccg 11100 agggacctga gcgagtccgc atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac 11160 cagtcacagt cgcaaggtag gctgagcacc gtggcgggcg gcagcgggcg gcggtcgggg 11220 ttgtttctgg cggaggtgct gctgatgatg taattaaagt aggcggtctt gagacggcgg 11280 atggtcgaca gaagcaccat gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc 11340 atgccccagg cttcgttttg acatcggcgc aggtctttgt agtagtcttg catgagcctt 11400 tctaccggca cttcttcttc tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg 11460 gcggcggcgg agtttggccg taggtggcgc cctcttcctc ccatgcgtgt gaccccgaag 11520 cccctcatcg gctgaagcag ggctaggtcg gcgacaacgc gctcggctaa tatggcctgc 11580 tgcacctgcg tgagggtaga ctggaagtca tccatgtcca caaagcggtg gtatgcgccc 11640 gtgttgatgg tgtaagtgca gttggccata acggaccagt taacggtctg gtgacccggc 11700 tgcgagagct cggtgtacct gagacgcgag taagccctcg agtcaaatac gtagtcgttg 11760 caagtccgca ccaggtactg gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg 11820 ggccagcgta gggtggccgg ggctccgggg gcgagatctt ccaacataag gcgatgatat 11880 ccgtagatgt acctggacat ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag 11940 tcgcggacgc ggttccagat gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc 12000 tggccggtca ggcgcgcgca atcgttgacg ctctagcgtg caaaaggaga gcctgtaagc 12060 gggcactctt ccgtggtctg gtggataaat tcgcaagggt atcatggcgg acgaccgggg 12120 ttcgagcccc gtatccggcc gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac 12180 ccaggtgtgc gacgtcagac aacgggggag tgctcctttt ggcttccttc caggcgcggc 12240 ggctgctgcg ctagcttttt tggccactgg ccgcgcgcag cgtaagcggt taggctggaa 12300 agcgaaagca ttaagtggct cgctccctgt agccggaggg ttattttcca agggttgagt 12360 cgcgggaccc ccggttcgag tctcggaccg gccggactgc ggcgaacggg ggtttgcctc 12420 cccgtcatgc aagaccccgc ttgcaaattc ctccggaaac agggacgagc cccttttttg 12480 cttttcccag atgcatccgg tgctgcggca gatgcgcccc cctcctcagc agcggcaaga 12540 gcaagagcag cggcagacat gcagggcacc ctcccctcct cctaccgcgt caggaggggc 12600 gacatccgcg gttgacgcgg cagcagatgg tgattacgaa cccccgcggc gccgggcccg 12660 gcactacctg gacttggagg agggcgaggg cctggcgcgg ctaggagcgc cctctcctga 12720 gcggcaccca agggtgcagc tgaagcgtga tacgcgtgag gcgtacgtgc cgcggcagaa 12780 cctgtttcgc gaccgcgagg gagaggagcc cgaggagatg cgggatcgaa agttccacgc 12840 agggcgcgag ctgcggcatg gcctgaatcg cgagcggttg ctgcgcgagg aggactttga 12900 gcccgacgcg cgaaccggga ttagtcccgc gcgcgcacac gtggcggccg ccgacctggt 12960 aaccgcatac gagcagacgg tgaaccagga gattaacttt caaaaaagct ttaacaacca 13020 cgtgcgtacg cttgtggcgc gcgaggaggt ggctatagga ctgatgcatc tgtgggactt 13080 tgtaagcgcg ctggagcaaa acccaaatag caagccgctc atggcgcagc tgttccttat 13140 agtgcagcac agcagggaca acgaggcatt cagggatgcg ctgctaaaca tagtagagcc 13200 cgagggccgc tggctgctcg atttgataaa catcctgcag agcatagtgg tgcaggagcg 13260 cagcttgagc ctggctgaca aggtggccgc catcaactat tccatgctta gcctgggcaa 13320 gttttacgcc cgcaagatat accatacccc ttacgttccc atagacaagg aggtaaagat 13380 cgaggggttc tacatgcgca tggcgctgaa ggtgcttacc ttgagcgacg acctgggcgt 13440 ttatcgcaac gagcgcatcc acaaggccgt gagcgtgagc cggcggcgcg agctcagcga 13500 ccgcgagctg atgcacagcc tgcaaagggc cctggctggc acgggcagcg gcgatagaga 13560 ggccgagtcc tactttgacg cgggcgctga cctgcgctgg gccccaagcc gacgcgccct 13620 ggaggcagct ggggccggac ctgggctggc ggtggcaccc gcgcgcgctg gcaacgtcgg 13680 cggcgtggag gaatatgacg aggacgatga gtacgagcca gaggacggcg agtactaagc 13740 ggtgatgttt ctgatcagat gatgcaagac gcaacggacc cggcggtgcg ggcggcgctg 13800 cagagccagc cgtccggcct taactccacg gacgactggc gccaggtcat ggaccgcatc 13860 atgtcgctga ctgcgcgcaa tcctgacgcg ttccggcagc agccgcaggc caaccggctc 13920 tccgcaattc tggaagcggt ggtcccggcg cgcgcaaacc ccacgcacga gaaggtgctg 13980 gcgatcgtaa acgcgctggc cgaaaacagg gccatccggc ccgacgaggc cggcctggtc 14040 tacgacgcgc tgcttcagcg cgtggctcgt tacaacagcg gcaacgtgca gaccaacctg 14100 gaccggctgg tgggggatgt gcgcgaggcc gtggcgcagc gtgagcgcgc gcagcagcag 14160 ggcaacctgg gctccatggt tgcactaaac gccttcctga gtacacagcc cgccaacgtg 14220 ccgcggggac aggaggacta caccaacttt gtgagcgcac tgcggctaat ggtgactgag 14280 acaccgcaaa gtgaggtgta ccagtctggg ccagactatt ttttccagac cagtagacaa 14340 ggcctgcaga ccgtaaacct gagccaggct ttcaaaaact tgcaggggct gtggggggtg 14400 cgggctccca caggcgaccg cgcgaccgtg tctagcttgc tgacgcccaa ctcgcgcctg 14460 ttgctgctgc taatagcgcc cttcacggac agtggcagcg tgtcccggga cacataccta 14520 ggtcacttgc tgacactgta ccgcgaggcc ataggtcagg cgcatgtgga cgagcatact 14580 ttccaggaga ttacaagtgt cagccgcgcg ctggggcagg aggacacggg cagcctggag 14640 gcaaccctaa actacctgct gaccaaccgg cggcagaaga tcccctcgtt gcacagttta 14700 aacagcgagg aggagcgcat tttgcgctac gtgcagcaga gcgtgagcct taacctgatg 14760 cgcgacgggg taacgcccag cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc 14820 atgtatgcct caaaccggcc gtttatcaac cgcctaatgg actacttgca tcgcgcggcc 14880 gccgtgaacc ccgagtattt caccaatgcc atcttgaacc cgcactggct accgccccct 14940 ggtttctaca ccgggggatt cgaggtgccc gagggtaacg atggattcct ctgggacgac 15000 atagacgaca gcgtgttttc cccgcaaccg cagaccctgc tagagttgca acagcgcgag 15060 caggcagagg cggcgctgcg aaaggaaagc ttccgcaggc caagcagctt gtccgatcta 15120 ggcgctgcgg ccccgcggtc agatgctagt agcccatttc caagcttgat agggtctctt 15180 accagcactc gcaccacccg cccgcgcctg ctgggcgagg aggagtacct aaacaactcg 15240 ctgctgcagc cgcagcgcga aaaaaacctg cctccggcat ttcccaacaa cgggatagag 15300 agcctagtgg acaagatgag tagatggaag acgtacgcgc aggagcacag ggacgtgcca 15360 ggcccgcgcc cgcccacccg tcgtcaaagg cacgaccgtc agcggggtct ggtgtgggag 15420 gacgatgact cggcagacga cagcagcgtc ctggatttgg gagggagtgg caacccgttt 15480 gcgcaccttc gccccaggct ggggagaatg ttttaaaaaa aaaaaagcat gatgcaaaat 15540 aaaaaactca ccaaggccat ggcaccgagc gttggttttc ttgtattccc cttagtatgc 15600 ggcgcgcggc gatgtatgag gaaggtcctc ctccctccta cgagagtgtg gtgagcgcgg 15660 cgccagtggc ggcggcgctg ggttctccct tcgatgctcc cctggacccg ccgtttgtgc 15720 ctccgcggta cctgcggcct accgggggga gaaacagcat ccgttactct gagttggcac 15780 ccctattcga caccacccgt gtgtacctgg tggacaacaa gtcaacggat gtggcatccc 15840 tgaactacca gaacgaccac agcaactttc tgaccacggt cattcaaaac aatgactaca 15900 gcccggggga ggcaagcaca cagaccatca atcttgacga ccggtcgcac tggggcggcg 15960 acctgaaaac catcctgcat accaacatgc caaatgtgaa cgagttcatg tttaccaata 16020 agtttaaggc gcgggtgatg gtgtcgcgct tgcctactaa ggacaatcag gtggagctga 16080 aatacgagtg ggtggagttc acgctgcccg agggcaacta ctccgagacc atgaccatag 16140 accttatgaa caacgcgatc gtggagcact acttgaaagt gggcagacag aacggggttc 16200 tggaaagcga catcggggta aagtttgaca cccgcaactt cagactgggg tttgaccccg 16260 tcactggtct tgtcatgcct ggggtatata caaacgaagc cttccatcca gacatcattt 16320 tgctgccagg atgcggggtg gacttcaccc acagccgcct gagcaacttg ttgggcatcc 16380 gcaagcggca acccttccag gagggcttta ggatcaccta cgatgatctg gagggtggta 16440 acattcccgc actgttggat gtggacgcct accaggcgag cttgaaagat gacaccgaac 16500 agggcggggg tggcgcaggc ggcagcaaca gcagtggcag cggcgcggaa gagaactcca 16560 acgcggcagc cgcggcaatg cagccggtgg aggacatgaa cgatcatgcc attcgcggcg 16620 acacctttgc cacacgggct gaggagaagc gcgctgaggc cgaagcagcg gccgaagctg 16680 ccgcccccgc tgcgcaaccc gaggtcgaga agcctcagaa gaaaccggtg atcaaacccc 16740 tgacagagga cagcaagaaa cgcagttaca acctaataag caatgacagc accttcaccc 16800 agtaccgcag ctggtacctt gcatacaact acggcgaccc tcagaccgga atccgctcat 16860 ggaccctgct ttgcactcct gacgtaacct gcggctcgga gcaggtctac tggtcgttgc 16920 cagacatgat gcaagacccc gtgaccttcc gctccacgcg ccagatcagc aactttccgg 16980 tggtgggcgc cgagctgttg cccgtgcact ccaagagctt ctacaacgac caggccgtct 17040 actcccaact catccgccag tttacctctc tgacccacgt gttcaatcgc tttcccgaga 17100 accagatttt ggcgcgcccg ccagccccca ccatcaccac cgtcagtgaa aacgttcctg 17160 ctctcacaga tcacgggacg ctaccgctgc gcaacagcat cggaggagtc cagcgagtga 17220 ccattactga cgccagacgc cgcacctgcc cctacgttta caaggccctg ggcatagtct 17280 cgccgcgcgt cctatcgagc cgcacttttt gagcaagcat gtccatcctt atatcgccca 17340 gcaataacac aggctggggc ctgcgcttcc caagcaagat gtttggcggg gccaagaagc 17400 gctccgacca acacccagtg cgcgtgcgcg ggcactaccg cgcgccctgg ggcgcgcaca 17460 aacgcggccg cactgggcgc accaccgtcg atgacgccat cgacgcggtg gtggaggagg 17520 cgcgcaacta cacgcccacg ccgccaccag tgtccacagt ggacgcggcc attcagaccg 17580 tggtgcgcgg agcccggcgc tatgctaaaa tgaagagacg gcggaggcgc gtagcacgtc 17640 gccaccgccg ccgacccggc actgccgccc aacgcgcggc ggcggccctg cttaaccgcg 17700 cacgtcgcac cggccgacgg gcggccatgc gggccgctcg aaggctggcc gcgggtattg 17760 tcactgtgcc ccccaggtcc aggcgacgag cggccgccgc agcagccgcg gccattagtg 17820 ctatgactca gggtcgcagg ggcaacgtgt attgggtgcg cgactcggtt agcggcctgc 17880 gcgtgcccgt gcgcacccgc cccccgcgca actagattgc aagaaaaaac tacttagact 17940 cgtactgttg tatgtatcca gcggcggcgg cgcgcaacga agctatgtcc aagcgcaaaa 18000 tcaaagaaga gatgctccag gtcatcgcgc cggagatcta tggccccccg aagaaggaag 18060 agcaggatta caagccccga aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgatg 18120 atgaacttga cgacgaggtg gaactgctgc acgctaccgc gcccaggcga cgggtacagt 18180 ggaaaggtcg acgcgtaaaa cgtgttttgc gacccggcac caccgtagtc tttacgcccg 18240 gtgagcgctc cacccgcacc tacaagcgcg tgtatgatga ggtgtacggc gacgaggacc 18300 tgcttgagca ggccaacgag cgcctcgggg agtttgccta cggaaagcgg cataaggaca 18360 tgctggcgtt gccgctggac gagggcaacc caacacctag cctaaagccc gtaacactgc 18420 agcaggtgct gcccgcgctt gcaccgtccg aagaaaagcg cggcctaaag cgcgagtctg 18480 gtgacttggc acccaccgtg cagctgatgg tacccaagcg ccagcgactg gaagatgtct 18540 tggaaaaaat gaccgtggaa cctgggctgg agcccgaggt ccgcgtgcgg ccaatcaagc 18600 aggtggcgcc gggactgggc gtgcagaccg tggacgttca gatacccact accagtagca 18660 ccagtattgc caccgccaca gagggcatgg agacacaaac gtccccggtt gcctcagcgg 18720 tggcggatgc cgcggtgcag gcggtcgctg cggccgcgtc caagacctct acggaggtgc 18780 aaacggaccc gtggatgttt cgcgtttcag ccccccggcg cccgcgccgt tcgaggaagt 18840 acggcgccgc cagcgcgcta ctgcccgaat atgccctaca tccttccatt gcgcctaccc 18900 ccggctatcg tggctacacc taccgcccca gaagacgagc aactacccga cgccgaacca 18960 ccactggaac ccgccgccgc cgtcgccgtc gccagcccgt gctggccccg atttccgtgc 19020 gcagggtggc tcgcgaagga ggcaggaccc tggtgctgcc aacagcgcgc taccacccca 19080 gcatcgttta aaagccggtc tttgtggttc ttgcagatat ggccctcacc tgccgcctcc 19140 gtttcccggt gccgggattc cgaggaagaa tgcaccgtag gaggggcatg gccggccacg 19200 gcctgacggg cggcatgcgt cgtgcgcacc accggcggcg gcgcgcgtcg caccgtcgca 19260 tgcgcggcgg tatcctgccc ctccttattc cactgatcgc cgcggcgatt ggcgccgtgc 19320 ccggaattgc atccgtggcc ttgcaggcgc agagacactg attaaaaaca agttgcatgt 19380 ggaaaaatca aaataaaaag tctggactct cacgctcgct tggtcctgta actattttgt 19440 agaatggaag acatcaactt tgcgtctctg gccccgcgac acggctcgcg cccgttcatg 19500 ggaaactggc aagatatcgg caccagcaat atgagcggtg gcgccttcag ctggggctcg 19560 ctgtggagcg gcattaaaaa tttcggttcc accgttaaga actatggcag caaggcctgg 19620 aacagcagca caggccagat gctgagggat aagttgaaag agcaaaattt ccaacaaaag 19680 gtggtagatg gcctggcctc tggcattagc ggggtggtgg acctggccaa ccaggcagtg 19740 caaaataaga ttaacagtaa gcttgatccc cgccctcccg tagaggagcc tccaccggcc 19800 gtggagacag tgtctccaga ggggcgtggc gaaaagcgtc cgcgccccga cagggaagaa 19860 actctggtga cgcaaataga cgagcctccc tcgtacgagg aggcactaaa gcaaggcctg 19920 cccaccaccc gtcccatcgc gcccatggct accggagtgc tgggccagca cacacccgta 19980 acgctggacc tgcctccccc cgccgacacc cagcagaaac ctgtgctgcc aggcccgacc 20040 gccgttgttg taacccgtcc tagccgcgcg tccctgcgcc gcgccgccag cggtccgcga 20100 tcgttgcggc ccgtagccag tggcaactgg caaagcacac tgaacagcat cgtgggtctg 20160 ggggtgcaat ccctgaagcg ccgacgatgc ttctgatagc taacgtgtcg tatgtgtgtc 20220 atgtatgcgt ccatgtcgcc gccagaggag ctgctgagcc gccgcgcgcc cgctttccaa 20280 gatggctacc ccttcgatga tgccgcagtg gtcttacatg cacatctcgg gccaggacgc 20340 ctcggagtac ctgagccccg ggctggtgca gtttgcccgc gccaccgaga cgtacttcag 20400 cctgaataac aagtttagaa accccacggt ggcgcctacg cacgacgtga ccacagaccg 20460 gtcccagcgt ttgacgctgc ggttcatccc tgtggaccgt gaggatactg cgtactcgta 20520 caaggcgcgg ttcaccctag ctgtgggtga taaccgtgtg ctggacatgg cttccacgta 20580 ctttgacatc cgcggcgtgc tggacagggg ccctactttt aagccctact ctggcactgc 20640 ctacaacgcc ctggctccca agggtgcccc aaatccttgc gaatgggatg aagctgctac 20700 tgctcttgaa ataaacctag aagaagagga cgatgacaac gaagacgaag tagacgagca 20760 agctgagcag caaaaaactc acgtatttgg gcaggcgcct tattctggta taaatattac 20820 aaaggagggt attcaaatag gtgtcgaagg tcaaacacct aaatatgccg ataaaacatt 20880 tcaacctgaa cctcaaatag gagaatctca gtggtacgaa acagaaatta atcatgcagc 20940 tgggagagtc ctaaaaaaga ctaccccaat gaaaccatgt tacggttcat atgcaaaacc 21000 cacaaatgaa aatggagggc aaggcattct tgtaaagcaa caaaatggaa agctagaaag 21060 tcaagtggaa atgcaatttt tctcaactac tgaggcagcc gcaggcaatg gtgataactt 21120 gactcctaaa gtggtattgt acagtgaaga tgtagatata gaaaccccag acactcatat 21180 ttcttacatg cccactatta aggaaggtaa ctcacgagaa ctaatgggcc aacaatctat 21240 gcccaacagg cctaattaca ttgcttttag ggacaatttt attggtctaa tgtattacaa 21300 cagcacgggt aatatgggtg ttctggcggg ccaagcatcg cagttgaatg ctgttgtaga 21360 tttgcaagac agaaacacag agctttcata ccagcttttg cttgattcca ttggtgatag 21420 aaccaggtac ttttctatgt ggaatcaggc tgttgacagc tatgatccag atgttagaat 21480 tattgaaaat catggaactg aagatgaact tccaaattac tgctttccac tgggaggtgt 21540 gattaataca gagactctta ccaaggtaaa acctaaaaca ggtcaggaaa atggatggga 21600 aaaagatgct acagaatttt cagataaaaa tgaaataaga gttggaaata attttgccat 21660 ggaaatcaat ctaaatgcca acctgtggag aaatttcctg tactccaaca tagcgctgta 21720 tttgcccgac aagctaaagt acagtccttc caacgtaaaa atttctgata acccaaacac 21780 ctacgactac atgaacaagc gagtggtggc tcccgggcta gtggactgct acattaacct 21840 tggagcacgc tggtcccttg actatatgga caacgtcaac ccatttaacc accaccgcaa 21900 tgctggcctg cgctaccgct caatgttgct gggcaatggt cgctatgtgc ccttccacat 21960 ccaggtgcct cagaagttct ttgccattaa aaacctcctt ctcctgccgg gctcatacac 22020 ctacgagtgg aacttcagga aggatgttaa catggttctg cagagctccc taggaaatga 22080 cctaagggtt gacggagcca gcattaagtt tgatagcatt tgcctttacg ccaccttctt 22140 ccccatggcc cacaacaccg cctccacgct tgaggccatg cttagaaacg acaccaacga 22200 ccagtccttt aacgactatc tctccgccgc caacatgctc taccctatac ccgccaacgc 22260 taccaacgtg cccatatcca tcccctcccg caactgggcg gctttccgcg gctgggcctt 22320 cacgcgcctt aagactaagg aaaccccatc actgggctcg ggctacgacc cttattacac 22380 ctactctggc tctataccct acctagatgg aaccttttac ctcaaccaca cctttaagaa 22440 ggtggccatt acctttgact cttctgtcag ctggcctggc aatgaccgcc tgcttacccc 22500 caacgagttt gaaattaagc gctcagttga cggggagggt tacaacgttg cccagtgtaa 22560 catgaccaaa gactggttcc tggtacaaat gctagctaac tataacattg gctaccaggg 22620 cttctatatc ccagagagct acaaggaccg catgtactcc ttctttagaa acttccagcc 22680 catgagccgt caggtggtgg atgatactaa atacaaggac taccaacagg tgggcatcct 22740 acaccaacac aacaactctg gatttgttgg ctaccttgcc cccaccatgc gcgaaggaca 22800 ggcctaccct gctaacttcc cctatccgct tataggcaag accgcagttg acagcattac 22860 ccagaaaaag tttctttgcg atcgcaccct ttggcgcatc ccattctcca gtaactttat 22920 gtccatgggc gcactcacag acctgggcca aaaccttctc tacgccaact ccgcccacgc 22980 gctagacatg acttttgagg tggatcccat ggacgagccc acccttcttt atgttttgtt 23040 tgaagtcttt gacgtggtcc gtgtgcacca gccgcaccgc ggcgtcatcg aaaccgtgta 23100 cctgcgcacg cccttctcgg ccggcaacgc cacaacataa agaagcaagc aacatcaaca 23160 acagctgccg ccatgggctc cagtgagcag gaactgaaag ccattgtcaa agatcttggt 23220 tgtgggccat attttttggg cacctatgac aagcgctttc caggctttgt ttctccacac 23280 aagctcgcct gcgccatagt caatacggcc ggtcgcgaga ctgggggcgt acactggatg 23340 gcctttgcct ggaacccgca ctcaaaaaca tgctacctct ttgagccctt tggcttttct 23400 gaccagcgac tcaagcaggt ttaccagttt gagtacgagt cactcctgcg ccgtagcgcc 23460 attgcttctt cccccgaccg ctgtataacg ctggaaaagt ccacccaaag cgtacagggg 23520 cccaactcgg ccgcctgtgg actattctgc tgcatgtttc tccacgcctt tgccaactgg 23580 ccccaaactc ccatggatca caaccccacc atgaacctta ttaccggggt acccaactcc 23640 atgctcaaca gtccccaggt acagcccacc ctgcgtcgca accaggaaca gctctacagc 23700 ttcctggagc gccactcgcc ctacttccgc agccacagtg cgcagattag gagcgccact 23760 tctttttgtc acttgaaaaa catgtaaaaa taatgtacta gagacacttt caataaaggc 23820 aaatgctttt atttgtacac tctcgggtga ttatttaccc ccacccttgc cgtctgcgcc 23880 gtttaaaaat caaaggggtt ctgccgcgca tcgctatgcg ccactggcag ggacacgttg 23940 cgatactggt gtttagtgct ccacttaaac tcaggcacaa ccatccgcgg cagctcggtg 24000 aagttttcac tccacaggct gcgcaccatc accaacgcgt ttagcaggtc gggcgccgat 24060 atcttgaagt cgcagttggg gcctccgccc tgcgcgcgcg agttgcgata cacagggttg 24120 cagcactgga acactatcag cgccgggtgg tgcacgctgg ccagcacgct cttgtcggag 24180 atcagatccg cgtccaggtc ctccgcgttg ctcagggcga acggagtcaa ctttggtagc 24240 tgccttccca aaaagggcgc gtgcccaggc tttgagttgc actcgcaccg tagtggcatc 24300 aaaaggtgac cgtgcccggt ctgggcgtta ggatacagcg cctgcataaa agccttgatc 24360 tgcttaaaag ccacctgagc ctttgcgcct tcagagaaga acatgccgca agacttgccg 24420 gaaaactgat tggccggaca ggccgcgtcg tgcacgcagc accttgcgtc ggtgttggag 24480 atctgcacca catttcggcc ccaccggttc ttcacgatct tggccttgct agactgctcc 24540 ttcagcgcgc gctgcccgtt ttcgctcgtc acatccattt caatcacgtg ctccttattt 24600 atcataatgc ttccgtgtag acacttaagc tcgccttcga tctcagcgca gcggtgcagc 24660 cacaacgcgc agcccgtggg ctcgtgatgc ttgtaggtca cctctgcaaa cgactgcagg 24720 tacgcctgca ggaatcgccc catcatcgtc acaaaggtct tgttgctggt gaaggtcagc 24780 tgcaacccgc ggtgctcctc gttcagccag gtcttgcata cggccgccag agcttccact 24840 tggtcaggca gtagtttgaa gttcgccttt agatcgttat ccacgtggta cttgtccatc 24900 agcgcgcgcg cagcctccat gcccttctcc cacgcagaca cgatcggcac actcagcggg 24960 ttcatcaccg taatttcact ttccgcttcg ctgggctctt cctcttcctc ttgcgtccgc 25020 ataccacgcg ccactgggtc gtcttcattc agccgccgca ctgtgcgctt acctcctttg 25080 ccatgcttga ttagcaccgg tgggttgctg aaacccacca tttgtagcgc cacatcttct 25140 ctttcttcct cgctgtccac gattacctct ggtgatggcg ggcgctcggg cttgggagaa 25200 gggcgcttct ttttcttctt gggcgcaatg gccaaatccg ccgccgaggt cgatggccgc 25260 gggctgggtg tgcgcggcac cagcgcgtct tgtgatgagt cttcctcgtc ctcggactcg 25320 atacgccgcc tcatccgctt ttttgggggc gcccggggag gcggcggcga cggggacggg 25380 gacgacacgt cctccatggt tgggggacgt cgcgccgcac cgcgtccgcg ctcgggggtg 25440 gtttcgcgct gctcctcttc ccgactggcc atttccttct cctataggca gaaaaagatc 25500 atggagtcag tcgagaagaa ggacagccta accgccccct ctgagttcgc caccaccgcc 25560 tccaccgatg ccgccaacgc gcctaccacc ttccccgtcg aggcaccccc gcttgaggag 25620 gaggaagtga ttatcgagca ggacccaggt tttgtaagcg aagacgacga ggaccgctca 25680 gtaccaacag aggataaaaa gcaagaccag gacaacgcag aggcaaacga ggaacaagtc 25740 gggcgggggg acgaaaggca tggcgactac ctagatgtgg gagacgacgt gctgttgaag 25800 catctgcagc gccagtgcgc cattatctgc gacgcgttgc aagagcgcag cgatgtgccc 25860 ctcgccatag cggatgtcag ccttgcctac gaacgccacc tattctcacc gcgcgtaccc 25920 cccaaacgcc aagaaaacgg cacatgcgag cccaacccgc gcctcaactt ctaccccgta 25980 tttgccgtgc cagaggtgct tgccacctat cacatctttt tccaaaactg caagataccc 26040 ctatcctgcc gtgccaaccg cagccgagcg gacaagcagc tggccttgcg gcagggcgct 26100 gtcatacctg atatcgcctc gctcaacgaa gtgccaaaaa tctttgaggg tcttggacgc 26160 gacgagaagc gcgcggcaaa cgctctgcaa caggaaaaca gcgaaaatga aagtcactct 26220 ggagtgttgg tggaactcga gggtgacaac gcgcgcctag ccgtactaaa acgcagcatc 26280 gaggtcaccc actttgccta cccggcactt aacctacccc ccaaggtcat gagcacagtc 26340 atgagtgagc tgatcgtgcg ccgtgcgcag cccctggaga gggatgcaaa tttgcaagaa 26400 caaacagagg agggcctacc cgcagttggc gacgagcagc tagcgcgctg gcttcaaacg 26460 cgcgagcctg ccgacttgga ggagcgacgc aaactaatga tggccgcagt gctcgttacc 26520 gtggagcttg agtgcatgca gcggttcttt gctgacccgg agatgcagcg caagctagag 26580 gaaacattgc actacacctt tcgacagggc tacgtacgcc aggcctgcaa gatctccaac 26640 gtggagctct gcaacctggt ctcctacctt ggaattttgc acgaaaaccg ccttgggcaa 26700 aacgtgcttc attccacgct caagggcgag gcgcgccgcg actacgtccg cgactgcgtt 26760 tacttatttc tatgctacac ctggcagacg gccatgggcg tttggcagca gtgcttggag 26820 gagtgcaacc tcaaggagct gcagaaactg ctaaagcaaa acttgaagga cctatggacg 26880 gccttcaacg agcgctccgt ggccgcgcac ctggcggaca tcattttccc cgaacgcctg 26940 cttaaaaccc tgcaacaggg tctgccagac ttcaccagtc aaagcatgtt gcagaacttt 27000 aggaacttta tcctagagcg ctcaggaatc ttgcccgcca cctgctgtgc acttcctagc 27060 gactttgtgc ccattaagta ccgcgaatgc cctccgccgc tttggggcca ctgctacctt 27120 ctgcagctag ccaactacct tgcctaccac tctgacataa tggaagacgt gagcggtgac 27180 ggtctactgg agtgtcactg tcgctgcaac ctatgcaccc cgcaccgctc cctggtttgc 27240 aattcgcagc tgcttaacga aagtcaaatt atcggtacct ttgagctgca gggtccctcg 27300 cctgacgaaa agtccgcggc tccggggttg aaactcactc cggggctgtg gacgtcggct 27360 taccttcgca aatttgtacc tgaggactac cacgcccacg agattaggtt ctacgaagac 27420 caatcccgcc cgcctaatgc ggagcttacc gcctgcgtca ttacccaggg ccacattctt 27480 ggccaattgc aagccatcaa caaagcccgc caagagtttc tgctacgaaa gggacggggg 27540 gtttacttgg acccccagtc cggcgaggag ctcaacccaa tccccccgcc gccgcagccc 27600 tatcagcagc agccgcgggc ccttgcttcc caggatggca cccaaaaaga agctgcagct 27660 gccgccgcca cccacggacg aggaggaata ctgggacagt caggcagagg aggttttgga 27720 cgaggaggag gaggacatga tggaagactg ggagagccta gacgaggaag cttccgaggt 27780 cgaagaggtg tcagacgaaa caccgtcacc ctcggtcgca ttcccctcgc cggcgcccca 27840 gaaatcggca accggttcca gcatggctac aacctccgct cctcaggcgc cgccggcact 27900 gcccgttcgc cgacccaacc gtagatggga caccactgga accagggccg gtaagtccaa 27960 gcagccgccg ccgttagccc aagagcaaca acagcgccaa ggctaccgct catggcgcgg 28020 gcacaagaac gccatagttg cttgcttgca agactgtggg ggcaacatct ccttcgcccg 28080 ccgctttctt ctctaccatc acggcgtggc cttcccccgt aacatcctgc attactaccg 28140 tcatctctac agcccatact gcaccggcgg cagcggcagc aacagcagcg gccacacaga 28200 agcaaaggcg accggatagc aagactctga caaagcccaa gaaatccaca gcggcggcag 28260 cagcaggagg aggagcgctg cgtctggcgc ccaacgaacc cgtatcgacc cgcgagctta 28320 gaaacaggat ttttcccact ctgtatgcta tatttcaaca gagcaggggc caagaacaag 28380 agctgaaaat aaaaaacagg tctctgcgat ccctcacccg cagctgcctg tatcacaaaa 28440 gcgaagatca gcttcggcgc acgctggaag acgcggaggc tctcttcagt aaatactgcg 28500 cgctgactct taaggactag tttcgcgccc tttctcaaat ttaagcgcga aaactacgtc 28560 atctccagcg gccacacccg gcgccagcac ctgttgtcag cgccattatg agcaaggaaa 28620 ttcccacgcc ctacatgtgg agttaccagc cacaaatggg acttgcggct ggagctgccc 28680 aagactactc aacccgaata aactacatga gcgcgggacc ccacatgata tcccgggtca 28740 acggaatacg cgcccaccga aaccgaattc tcctggaaca ggcggctatt accaccacac 28800 ctcgtaataa ccttaatccc cgtagttggc ccgctgccct ggtgtaccag gaaagtcccg 28860 ctcccaccac tgtggtactt cccagagacg cccaggccga agttcagatg actaactcag 28920 gggcgcagct tgcgggcggc tttcgtcaca gggtgcggtc gcccgggcag ggtataactc 28980 acctgacaat cagagggcga ggtattcagc tcaacgacga gtcggtgagc tcctcgcttg 29040 gtctccgtcc ggacgggaca tttcagatcg gcggcgccgg ccgctcttca ttcacgcctc 29100 gtcaggcaat cctaactctg cagacctcgt cctctgagcc gcgctctgga ggcattggaa 29160 ctctgcaatt tattgaggag tttgtgccat cggtctactt taaccccttc tcgggacctc 29220 ccggccacta tccggatcaa tttattccta actttgacgc ggtaaaggac tcggcggacg 29280 gctacgactg aatgttaagt ggagaggcag agcaactgcg cctgaaacac ctggtccact 29340 gtcgccgcca caagtgcttt gcccgcgact ccggtgagtt ttgctacttt gaattgcccg 29400 aggatcatat cgagggcccg gcgcacggcg tccggcttac cgcccaggga gagcttgccc 29460 gtagcctgat tcgggagttt acccagcgcc ccctgctagt tgagcgggac aggggaccct 29520 gtgttctcac tgtgatttgc aactgtccta accctggatt acatcaagat cctctagtta 29580 atgtcaggtc gcctaagtcg attaactaga gtacccgggg atcttattcc ctttaactaa 29640 taaaaaaaaa taataaagca tcacttactt aaaatcagtt agcaaatttc tgtccagttt 29700 attcagcagc acctccttgc cctcctccca gctctggtat tgcagcttcc tcctggctgc 29760 aaactttctc cacaatctaa atggaatgtc agtttcctcc tgttcctgtc catccgcacc 29820 cactatcttc atgttgttgc agatgaagcg cgcaagaccg tctgaagata ccttcaaccc 29880 cgtgtatcca tatgacacgg aaaccggtcc tccaactgtg ccttttctta ctcctccctt 29940 tgtatccccc aatgggtttc aagagagtcc ccctggggta ctctctttgc gcctatccga 30000 acctctagtt acctccaatg gcatgcttgc gctcaaaatg ggcaacggcc tctctctgga 30060 cgaggccggc aaccttacct cccaaaatgt aaccactgtg agcccacctc tcaaaaaaac 30120 caagtcaaac ataaacctgg aaatatctgc acccctcaca gttacctcag aagccctaac 30180 tgtggctgcc gccgcacctc taatggtcgc gggcaacaca ctcaccatgc aatcacaggc 30240 cccgctaacc gtgcacgact ccaaacttag cattgccacc caaggacccc tcacagtgtc 30300 agaaggaaag ctagccctgc aaacatcagg ccccctcacc accaccgata gcagtaccct 30360 tactatcact gcctcacccc ctctaactac tgccactggt agcttgggca ttgacttgaa 30420 agagcccatt tatacacaaa atggaaaact aggactaaag tacggggctc ctttgcatgt 30480 aacagacgac ctaaacactt tgaccgtagc aactggtcca ggtgtgacta ttaataatac 30540 ttccttgcaa actaaagtta ctggagcctt gggttttgat tcacaaggca atatgcaact 30600 taatgtagca ggaggactaa ggattgattc tcaaaacaga cgccttatac ttgatgttag 30660 ttatccgttt gatgctcaaa accaactaaa tctaagacta ggacagggcc ctctttttat 30720 aaactcagcc cacaacttgg atattaacta caacaaaggc ctttacttgt ttacagcttc 30780 aaacaattcc aaaaagcttg aggttaacct aagcactgcc aaggggttga tgtttgacgc 30840 tacagccata gccattaatg caggagatgg gcttgaattt ggttcaccta atgcaccaaa 30900 cacaaatccc ctcaaaacaa aaattggcca tggcctagaa tttgattcaa acaaggctat 30960 ggttcctaaa ctaggaactg gccttagttt tgacagcaca ggtgccatta cagtaggaaa 31020 caaaaataat gataagctaa ctttgtggac cggaataaac cctccaccta actgtcaaat 31080 tgtggaaaac actaatacaa atgatggcaa acttacttta gtattagtaa aaaatggagg 31140 gcttgttaat ggctacgtgt ctctagttgg tgtatcagac actgtgaacc aaatgttcac 31200 acaaaagaca gcaaacatcc aattaagatt atattttgac tcttctggaa atctattaac 31260 tgaggaatca gacttaaaaa ttccacttaa aaataaatct tctacagcga ccagtgaaac 31320 tgtagccagc agcaaagcct ttatgccaag tactacagct tatcccttca acaccactac 31380 tagggatagt gaaaactaca ttcatggaat atgttactac atgactagtt atgatagaag 31440 tctatttccc ttgaacattt ctataatgct aaacagccgt atgatttctt ccaatgttgc 31500 ctatgccata caatttgaat ggaatctaaa tgcaagtgaa tctccagaaa gcaacatagc 31560 tacgctgacc acatccccct ttttcttttc ttacattaca gaagacgaca actaaagaat 31620 cgtttgtgtt atgtttcaac gtgtttattt ttcaattgca gaaaatttca agtcattttt 31680 cattcagtag tatagcccca ccaccacata gcttatacag atcaccgtac cttaatcaaa 31740 ctcacagaac cctagtattc aacctgccac ctccctccca acacacagag tacacagtcc 31800 tttctccccg gctggcctta aaaagcatca tatcatgggt aacagacata ttcttaggtg 31860 ttatattcca cacggtttcc tgtcgagcca aacgctcatc agtgatatta ataaactccc 31920 cgggcagctc acttaagttc atgtcgctgt ccagctgctg agccacaggc tgctgtccaa 31980 cttgcggttg cttaacgggc ggcgaaggag aagtccacgc ctacatgggg gtagagtcat 32040 aatcgtgcat caggataggg cggtggtgct gcagcagcgc gcgaataaac tgctgccgcc 32100 gccgctccgt cctgcaggaa tacaacatgg cagtggtctc ctcagcgatg attcgcaccg 32160 cccgcagcat aaggcgcctt gtcctccggg cacagcagcg caccctgatc tcacttaaat 32220 cagcacagta actgcagcac agcaccacaa tattgttcaa aatcccacag tgcaaggcgc 32280 tgtatccaaa gctcatggcg gggaccacag aacccacgtg gccatcatac cacaagcgca 32340 ggtagattaa gtggcgaccc ctcataaaca cgctggacat aaacattacc tcttttggca 32400 tgttgtaatt caccacctcc cggtaccata taaacctctg attaaacatg gcgccatcca 32460 ccaccatcct aaaccagctg gccaaaacct gcccgccggc tatacactgc agggaaccgg 32520 gactggaaca atgacagtgg agagcccagg actcgtaacc atggatcatc atgctcgtca 32580 tgatatcaat gttggcacaa cacaggcaca cgtgcataca cttcctcagg attacaagct 32640 cctcccgcgt tagaaccata tcccagggaa caacccattc ctgaatcagc gtaaatccca 32700 cactgcaggg aagacctcgc acgtaactca cgttgtgcat tgtcaaagtg ttacattcgg 32760 gcagcagcgg atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac 32820 gatccctact gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc 32880 caaatggaac gccggacgta gtcatatttc ctgaagcaaa accaggtgcg ggcgtgacaa 32940 acagatctgc gtctccggtc tcgccgctta gatcgctctg tgtagtagtt gtagtatatc 33000 cactctctca aagcatccag gcgccccctg gcttcgggtt ctatgtaaac tccttcatgc 33060 gccgctgccc tgataacatc caccaccgca gaataagcca cacccagcca acctacacat 33120 tcgttctgcg agtcacacac gggaggagcg ggaagagctg gaagaaccat gttttttttt 33180 ttattccaaa agattatcca aaacctcaaa atgaagatct attaagtgaa cgcgctcccc 33240 tccggtggcg tggtcaaact ctacagccaa agaacagata atggcatttg taagatgttg 33300 cacaatggct tccaaaaggc aaacggccct cacgtccaag tggacgtaaa ggctaaaccc 33360 ttcagggtga atctcctcta taaacattcc agcaccttca accatgccca aataattctc 33420 atctcgccac cttctcaata tatctctaag caaatcccga atattaagtc cggccattgt 33480 aaaaatctgc tccagagcgc cctccacctt cagcctcaag cagcgaatca tgattgcaaa 33540 aattcaggtt cctcacagac ctgtataaga ttcaaaagcg gaacattaac aaaaataccg 33600 cgatcccgta ggtcccttcg cagggccagc tgaacataat cgtgcaggtc tgcacggacc 33660 agcgcggcca cttccccgcc aggaaccatg acaaaagaac ccacactgat tatgacacgc 33720 atactcggag ctatgctaac cagcgtagcc ccgatgtaag cttgttgcat gggcggcgat 33780 ataaaatgca aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa agaaagcaca 33840 tcgtagtcat gctcatgcag ataaaggcag gtaagctccg gaaccaccac agaaaaagac 33900 accatttttc tctcaaacat gtctgcgggt ttctgcataa acacaaaata aaataacaaa 33960 aaaacattta aacattagaa gcctgtctta caacaggaaa aacaaccctt ataagcataa 34020 gacggactac ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca 34080 ccaccgacag ctcctcggtc atgtccggag tcataatgta agactcggta aacacatcag 34140 gttgattcac atcggtcagt gctaaaaagc gaccgaaata gcccggggga atacataccc 34200 gcaggcgtag agacaacatt acagccccca taggaggtat aacaaaatta ataggagaga 34260 aaaacacata aacacctgaa aaaccctcct gcctaggcaa aatagcaccc tcccgctcca 34320 gaacaacata cagcgcttcc acagcggcag ccataacagt cagccttacc agtaaaaaag 34380 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa 34440 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc 34500 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc 34560 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtcacttcc cattttaaga 34620 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg 34680 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc 34740 caaaataagg tatattattg atgatg 34766 <210> 30 <211> 35726 <212> DNA <213> Artificial Sequence <220> <223> Adk35F2-Spike/E4orf6(mPGK)-E4dl-711(E4re#29) <400> 30 ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg ataatgaggg 60 ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc 120 ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga 180 cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga 240 tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa 300 taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata atttctgtaa 360 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 420 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 480 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 540 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 600 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 660 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 720 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 780 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 840 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 900 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat 960 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 1020 gacctccata gaagacaccg ggaccgatcc agcctccgat ttgccaccat gtttgtgttc 1080 ctggtgctgc tgccactggt gtccagccag tgtgtgaacc tgaccaccag gacccaactt 1140 cctcctgcct acaccaactc cttcaccagg ggagtctact accctgacaa ggtgttcagg 1200 tcctctgtgc tgcacagcac ccaggacctg ttcctgccat tcttcagcaa tgtgacctgg 1260 ttccatgcca tccatgtgtc tggcaccaat ggcaccaaga ggtttgacaa ccctgtgctg 1320 ccattcaatg atggagtcta ctttgccagc acagagaaga gcaacatcat caggggctgg 1380 atttttggca ccaccctgga cagcaagacc cagtccctgc tgattgtgaa caatgccacc 1440 aatgtggtga ttaaggtgtg tgagttccag ttctgtaatg acccattcct gggagtctac 1500 taccacaaga acaacaagtc ctggatggag tctgagttca gggtctactc ctctgccaac 1560 aactgtacct ttgaatatgt gagccaacca ttcctgatgg acttggaggg caagcagggc 1620 aacttcaaga acctgaggga gtttgtgttc aagaacattg atggctactt caagatttac 1680 agcaaacaca caccaatcaa cctggtgagg gacctgccac agggcttctc tgccttggaa 1740 ccactggtgg acctgccaat tggcatcaac atcaccaggt tccagaccct gctggctctg 1800 cacaggtcct acctgacacc tggagactcc tcctctggct ggacagcagg agcagcagcc 1860 tactatgtgg gctacctcca accaaggacc ttcctgctga aatacaatga gaatggcacc 1920 atcacagatg ctgtggactg tgccctggac ccactgtctg agaccaagtg taccctgaaa 1980 tccttcacag tggagaaggg catctaccag accagcaact tcagggtcca accaacagag 2040 agcattgtga ggtttccaaa catcaccaac ctgtgtccat ttggagaggt gttcaatgcc 2100 accaggtttg cctctgtcta tgcctggaac aggaagagga ttagcaactg tgtggctgac 2160 tactctgtgc tctacaactc tgcctccttc agcaccttca agtgttatgg agtgagccca 2220 accaaactga atgacctgtg tttcaccaat gtctatgctg actcctttgt gattagggga 2280 gatgaggtga gacagattgc ccctggacaa acaggcaaga ttgctgacta caactacaaa 2340 ctgcctgatg acttcacagg ctgtgtgatt gcctggaaca gcaacaacct ggacagcaag 2400 gtgggaggca actacaacta cctctacaga ctgttcagga agagcaacct gaaaccattt 2460 gagagggaca tcagcacaga gatttaccag gctggcagca caccatgtaa tggagtggag 2520 ggcttcaact gttactttcc actccaatcc tatggcttcc aaccaaccaa tggagtgggc 2580 taccaaccat acagggtggt ggtgctgtcc tttgaactgc tccatgcccc tgccacagtg 2640 tgtggaccaa agaagagcac caacctggtg aagaacaagt gtgtgaactt caacttcaat 2700 ggactgacag gcacaggagt gctgacagag agcaacaaga agttcctgcc attccaacag 2760 tttggcaggg acattgctga caccacagat gctgtgaggg acccacagac cttggagatt 2820 ctggacatca caccatgttc ctttggagga gtgtctgtga ttacacctgg caccaacacc 2880 agcaaccagg tggctgtgct ctaccaggat gtgaactgta ctgaggtgcc tgtggctatc 2940 catgctgacc aacttacacc aacctggagg gtctacagca caggcagcaa tgtgttccag 3000 accagggctg gctgtctgat tggagcagag catgtgaaca actcctatga gtgtgacatc 3060 ccaattggag caggcatctg tgcctcctac cagacccaga ccggtggcgg tgggtcgagg 3120 tctgtggcaa gccagagcat cattgcctac acaatgagtc tgggagcaga gaactctgtg 3180 gcttacagca acaacagcat tgccatccca accaacttca ccatctctgt gaccacagag 3240 attctgcctg tgagtatgac caagacctct gtggactgta caatgtatat ctgtggagac 3300 agcacagagt gtagcaacct gctgctccaa tatggctcct tctgtaccca acttaacagg 3360 gctctgacag gcattgctgt ggaacaggac aagaacaccc aggaggtgtt tgcccaggtg 3420 aagcagattt acaagacacc tccaatcaag gactttggag gcttcaactt cagccagatt 3480 ctgcctgacc caagcaagcc aagcaagagg tccttcattg aggacctgct gttcaacaag 3540 gtgaccctgg ctgatgctgg cttcatcaag caatatggag actgtctggg agacattgct 3600 gccagggacc tgatttgtgc ccagaagttc aatggactga cagtgctgcc tccactgctg 3660 acagatgaga tgattgccca atacacctct gccctgctgg ctggcaccat cacctctggc 3720 tggacctttg gagcaggagc agccctccaa atcccatttg ctatgcagat ggcttacagg 3780 ttcaatggca ttggagtgac ccagaatgtg ctctatgaga accagaaact gattgccaac 3840 cagttcaact ctgccattgg caagattcag gactccctgt ccagcacagc ctctgccctg 3900 ggcaaactcc aagatgtggt gaaccagaat gcccaggctc tgaacaccct ggtgaagcaa 3960 ctttccagca actttggagc catctcctct gtgctgaatg acatcctgag cagactggac 4020 aaggtggagg ctgaggtcca gattgacaga ctgattacag gcagactcca atccctccaa 4080 acctatgtga cccaacaact tatcagggct gctgagatta gggcatctgc caacctggct 4140 gccaccaaga tgagtgagtg tgtgctggga caaagcaaga gggtggactt ctgtggcaag 4200 ggctaccacc tgatgagttt tccacagtct gcccctcatg gagtggtgtt cctgcatgtg 4260 acctatgtgc ctgcccagga gaagaacttc accacagccc ctgccatctg ccatgatggc 4320 aaggctcact ttccaaggga gggagtgttt gtgagcaatg gcacccactg gtttgtgacc 4380 cagaggaact tctatgaacc acagattatc accacagaca acacctttgt gtctggcaac 4440 tgtgatgtgg tgattggcat tgtgaacaac acagtctatg acccactcca acctgaactg 4500 gactccttca aggaggaact ggacaaatac ttcaagaacc acaccagccc tgatgtggac 4560 ctgggagaca tctctggcat caatgcctct gtggtgaaca tccagaagga gattgacaga 4620 ctgaatgagg tggctaagaa cctgaatgag tccctgattg acctccaaga actgggcaaa 4680 tatgaacaat acatcaagtg gccatgaaaa ttgatcataa tcagccatac cacatttgta 4740 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 4800 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4860 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4920 aaactcatca atgtatctta acgcggatcg ggtaggggag gcgcttttcc caaggcagtc 4980 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 5040 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 5100 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 5160 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 5220 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 5280 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 5340 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 5400 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga aactacccca 5460 agctggccac catgactacg tccggcgttc catttggcat gacactacga ccaacacgat 5520 ctcggttgtc tcggcgcact ccgtacagta gggatcgtct acctcctttt gagacagaaa 5580 cccgcgctac catactggag gatcatccgc tgctgcccga atgtaacact ttgacaatgc 5640 acaacgtgag ttacgtgcga ggtcttccct gcagtgtggg atttacgctg attcaggaat 5700 gggttgttcc ctgggatatg gttctaacgc gggaggagct tgtaatcctg aggaagtgta 5760 tgcacgtgtg cctgtgttgt gccaacattg atatcatgac gagcatgatg atccatggtt 5820 acgagtcctg ggctctccac tgtcattgtt ccagtcccgg ttccctgcag tgtatagccg 5880 gcgggcaggt tttggccagc tggtttagga tggtggtgga tggcgccatg tttaatcaga 5940 ggtttatatg gtaccgggag gtggtgaatt acaacatgcc aaaagaggta atgtttatgt 6000 ccagcgtgtt tatgaggggt cgccacttaa tctacctgcg cttgtggtat gatggccacg 6060 tgggttctgt ggtccccgcc atgagctttg gatacagcgc cttgcactgt gggattttga 6120 acaatattgt ggtgctgtgc tgcagttact gtgctgattt aagtgagatc agggtgcgct 6180 gctgtgcccg gaggacaagg cgccttatgc tgcgggcggt gcgaatcatc gctgaggaga 6240 ccactgccat gttgtattcc tgcaggacgg agcggcggcg gcagcagttt attcgcgcgc 6300 tgctgcagca ccaccgccct atcctgatgc acgattatga ctctaccccc atgtagaagc 6360 ttggggatca attctctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc 6420 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 6480 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 6540 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 6600 ctggggatgc ggtgggctct atggcttctg aggaaattgg gcgtggctta agggtgggaa 6660 agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg 6720 ccatgagcac caactcgttt gatggaagca ttgtgagctc atatttgaca acgcgcatgc 6780 ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt cgccccgtcc 6840 tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg ttggagactg 6900 cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg actgactttg 6960 ctttcctgag cccgcttgca agcagtgcag cttcccgttc atccgcccgc gatgacaagt 7020 tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc gtttctcagc 7080 agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct cccaatgcgg 7140 tttaaaacat aaataaaaaa ccagactctg tttggatttg gatcaagcaa gtgtcttgct 7200 gtctttattt aggggttttg cgcgcgcggt aggcccggga ccagcggtct cggtcgttga 7260 gggtcctgtg tattttttcc aggacgtggt aaaggtgact ctggatgttc agatacatgg 7320 gcataagccc gtctctgggg tggaggtagc accactgcag agcttcatgc tgcggggtgg 7380 tgttgtagat gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa atgtctttca 7440 gtagcaagct gattgccagg ggcaggccct tggtgtaagt gtttacaaag cggttaagct 7500 gggatgggtg catacgtggg gatatgagat gcatcttgga ctgtattttt aggttggcta 7560 tgttcccagc catatccctc cggggattca tgttgtgcag aaccaccagc acagtgtatc 7620 cggtgcactt gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag aacttggaga 7680 cgcccttgtg acctccaaga ttttccatgc attcgtccat aatgatggca atgggcccac 7740 gggcggcggc ctgggcgaag atatttctgg gatcactaac gtcatagttg tgttccagga 7800 tgagatcgtc ataggccatt tttacaaagc gcgggcggag ggtgccagac tgcggtataa 7860 tggttccatc cggcccaggg gcgtagttac cctcacagat ttgcatttcc cacgctttga 7920 gttcagatgg ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt tccggggtag 7980 gggagatcag ctgggaagaa agcaggttcc tgagcagctg cgacttaccg cagccggtgg 8040 gcccgtaaat cacacctatt accgggtgca actggtagtt aagagagctg cagctgccgt 8100 catccctgag caggggggcc acttcgttaa gcatgtccct gactcgcatg ttttccctga 8160 ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag gaagcaaagt 8220 ttttcaacgg tttgagaccg tccgccgtag gcatgctttt gagcgtttga ccaagcagtt 8280 ccaggcggtc ccacagctcg gtcacctgct ctacggcatc tcgatccagc atatctcctc 8340 gtttcgcggg ttggggcggc tttcgctgta cggcagtagt cggtgctcgt ccagacgggc 8400 cagggtcatg tctttccacg ggcgcagggt cctcgtcagc gtagtctggg tcacggtgaa 8460 ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc tgctggtgct 8520 gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag catttgacca tggtgtcata 8580 gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg cccttggagg aggcgccgca 8640 cgaggggcag tgcagacttt tgagggcgta gagcttgggc gcgagaaata ccgattccgg 8700 ggagtaggca tccgcgccgc aggccccgca gacggtctcg cattccacga gccaggtgag 8760 ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc gtttcttacc 8820 tctggtttcc atgagccggt gtccacgctc ggtgacgaaa aggctgtccg tgtccccgta 8880 tacagacttg agaggcctgt cctcgagcgg tgttccgcgg tcctcctcgt atagaaactc 8940 ggaccactct gagacaaagg ctcgcgtcca ggccagcacg aaggaggcta agtgggaggg 9000 gtagcggtcg ttgtccacta gggggtccac tcgctccagg gtgtgaagac acatgtcgcc 9060 ctcttcggca tcaaggaagg tgattggttt gtaggtgtag gccacgtgac cgggtgttcc 9120 tgaagggggg ctataaaagg gggtgggggc gcgttcgtcc tcactctctt ccgcatcgct 9180 gtctgcgagg gccagctgtt ggggtgagta ctccctctga aaagcgggca tgacttctgc 9240 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat 9300 gcctttgagg gtggccgcat ccatctggtc agaaaagaca atctttttgt tgtcaagctt 9360 ggtggcaaac gacccgtaga gggcgttgga cagcaacttg gcgatggagc gcagggtttg 9420 gtttttgtcg cgatcggcgc gctccttggc cgcgatgttt agctgcacgt attcgcgcgc 9480 aacgcaccgc cattcgggaa agacggtggt gcgctcgtcg ggcaccaggt gcacgcgcca 9540 accgcggttg tgcagggtga caaggtcaac gctggtggct acctctccgc gtaggcgctc 9600 gttggtccag cagaggcggc cgcccttgcg cgagcagaat ggcggtaggg ggtctagctg 9660 cgtctcgtcc ggggggtctg cgtccacggt aaagaccccg ggcagcaggc gcgcgtcgaa 9720 gtagtctatc ttgcatcctt gcaagtctag cgcctgctgc catgcgcggg cggcaagcgc 9780 gcgctcgtat gggttgagtg ggggacccca tggcatgggg tgggtgagcg cggaggcgta 9840 catgccgcaa atgtcgtaaa cgtagagggg ctctctgagt attccaagat atgtagggta 9900 gcatcttcca ccgcggatgc tggcgcgcac gtaatcgtat agttcgtgcg agggagcgag 9960 gaggtcggga ccgaggttgc tacgggcggg ctgctctgct cggaagacta tctgcctgaa 10020 gatggcatgt gagttggatg atatggttgg acgctggaag acgttgaagc tggcgtctgt 10080 gagacctacc gcgtcacgca cgaaggaggc gtaggagtcg cgcagcttgt tgaccagctc 10140 ggcggtgacc tgcacgtcta gggcgcagta gtccagggtt tccttgatga tgtcatactt 10200 atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc ggtctttcca 10260 gtactcttgg atcggaaacc cgtcggcctc cgaacggtaa gagcctagca tgtagaactg 10320 gttgacggcc tggtaggcgc agcatccctt ttctacgggt agcgcgtatg cctgcgcggc 10380 cttccggagc gaggtgtggg tgagcgcaaa ggtgtccctg accatgactt tgaggtactg 10440 gtatttgaag tcagtgtcgt cgcatccgcc ctgctcccag agcaaaaagt ccgtgcgctt 10500 tttggaacgc ggatttggca gggcgaaggt gacatcgttg aagagtatct ttcccgcgcg 10560 aggcataaag ttgcgtgtga tgcggaaggg tcccggcacc tcggaacggt tgttaattac 10620 ctgggcggcg agcacgatct cgtcaaagcc gttgatgttg tggcccacaa tgtaaagttc 10680 caagaagcgc gggatgccct tgatggaagg caatttttta agttcctcgt aggtgagctc 10740 ttcaggggag ctgagcccgt gctctgaaag ggcccagtct gcaagatgag ggttggaagc 10800 gacgaatgag ctccacaggt cacgggccat tagcatttgc aggtggtcgc gaaaggtcct 10860 aaactggcga cctatggcca ttttttctgg ggtgatgcag tagaaggtaa gcgggtcttg 10920 ttcccagcgg tcccatccaa ggttcgcggc taggtctcgc gcggcagtca ctagaggctc 10980 atctccgccg aacttcatga ccagcatgaa gggcacgagc tgcttcccaa aggcccccat 11040 ccaagtatag gtctctacat cgtaggtgac aaagagacgc tcggtgcgag gatgcgagcc 11100 gatcgggaag aactggatct cccgccacca attggaggag tggctattga tgtggtgaaa 11160 gtagaagtcc ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac gtgcgcagta 11220 ctggcagcgg tgcacgggct gtacatcctg cacgaggttg acctgacgac cgcgcacaag 11280 gaagcagagt gggaatttga gcccctcgcc tggcgggttt ggctggtggt cttctacttc 11340 ggctgcttgt ccttgaccgt ctggctgctc gaggggagtt acggtggatc ggaccaccac 11400 gccgcgcgag cccaaagtcc agatgtccgc gcgcggcggt cggagcttga tgacaacatc 11460 gcgcagatgg gagctgtcca tggtctggag ctcccgcggc gtcaggtcag gcgggagctc 11520 ctgcaggttt acctcgcata gacgggtcag ggcgcgggct agatccaggt gatacctaat 11580 ttccaggggc tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc cccgcggcgc 11640 gactacggta ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg atgcatctaa 11700 aagcggtgac gcgggcgagc ccccggaggt agggggggct ccggacccgc cgggagaggg 11760 ggcaggggca cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg taggttgctg 11820 gcgaacgcga cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt gaagacgacg 11880 ggcccggtga gcttgaacct gaaagagagt tcgacagaat caatttcggt gtcgttgacg 11940 gcggcctggc gcaaaatctc ctgcacgtct cctgagttgt cttgataggc gatctcggcc 12000 atgaactgct cgatctcttc ctcctggaga tctccgcgtc cggctcgctc cacggtggcg 12060 gcgaggtcgt tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc tccctcgttc 12120 cagacgcggc tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac cacctgcgcg 12180 agattgagct ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg aaagaggtag 12240 ttgagggtgg tggcggtgtg ttctgccacg aagaagtaca taacccagcg tcgcaacgtg 12300 gattcgttga tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa gtccacggcg 12360 aagttgaaaa actgggagtt gcgcgccgac acggttaact cctcctccag aagacggatg 12420 agctcggcga cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc ttcttcttct 12480 tcaatctcct cttccataag ggcctcccct tcttcttctt ctggcggcgg tgggggaggg 12540 gggacacggc ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc gatcatctcc 12600 ccgcggcgac ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg gcgcagttgg 12660 aagacgccgc ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg cggcagggat 12720 acggcgctaa cgatgcatct caacaattgt tgtgtaggta ctccgccgcc gagggacctg 12780 agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag 12840 tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg gttgtttctg 12900 gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgac 12960 agaagcacca tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag 13020 gcttcgtttt gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc 13080 acttcttctt ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg 13140 gagtttggcc gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa gcccctcatc 13200 ggctgaagca gggctaggtc ggcgacaacg cgctcggcta atatggcctg ctgcacctgc 13260 gtgagggtag actggaagtc atccatgtcc acaaagcggt ggtatgcgcc cgtgttgatg 13320 gtgtaagtgc agttggccat aacggaccag ttaacggtct ggtgacccgg ctgcgagagc 13380 tcggtgtacc tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt gcaagtccgc 13440 accaggtact ggtatcccac caaaaagtgc ggcggcggct ggcggtagag gggccagcgt 13500 agggtggccg gggctccggg ggcgagatct tccaacataa ggcgatgata tccgtagatg 13560 tacctggaca tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa gtcgcggacg 13620 cggttccaga tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct ctggccggtc 13680 aggcgcgcgc aatcgttgac gctctagcgt gcaaaaggag agcctgtaag cgggcactct 13740 tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg gttcgagccc 13800 cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa cccaggtgtg 13860 cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg cggctgctgc 13920 gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga aagcgaaagc 13980 attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag tcgcgggacc 14040 cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgcct ccccgtcatg 14100 caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt gcttttccca 14160 gatgcatccg gtgctgcggc agatgcgccc ccctcctcag cagcggcaag agcaagagca 14220 gcggcagaca tgcagggcac cctcccctcc tcctaccgcg tcaggagggg cgacatccgc 14280 ggttgacgcg gcagcagatg gtgattacga acccccgcgg cgccgggccc ggcactacct 14340 ggacttggag gagggcgagg gcctggcgcg gctaggagcg ccctctcctg agcggcaccc 14400 aagggtgcag ctgaagcgtg atacgcgtga ggcgtacgtg ccgcggcaga acctgtttcg 14460 cgaccgcgag ggagaggagc ccgaggagat gcgggatcga aagttccacg cagggcgcga 14520 gctgcggcat ggcctgaatc gcgagcggtt gctgcgcgag gaggactttg agcccgacgc 14580 gcgaaccggg attagtcccg cgcgcgcaca cgtggcggcc gccgacctgg taaccgcata 14640 cgagcagacg gtgaaccagg agattaactt tcaaaaaagc tttaacaacc acgtgcgtac 14700 gcttgtggcg cgcgaggagg tggctatagg actgatgcat ctgtgggact ttgtaagcgc 14760 gctggagcaa aacccaaata gcaagccgct catggcgcag ctgttcctta tagtgcagca 14820 cagcagggac aacgaggcat tcagggatgc gctgctaaac atagtagagc ccgagggccg 14880 ctggctgctc gatttgataa acatcctgca gagcatagtg gtgcaggagc gcagcttgag 14940 cctggctgac aaggtggccg ccatcaacta ttccatgctt agcctgggca agttttacgc 15000 ccgcaagata taccataccc cttacgttcc catagacaag gaggtaaaga tcgaggggtt 15060 ctacatgcgc atggcgctga aggtgcttac cttgagcgac gacctgggcg tttatcgcaa 15120 cgagcgcatc cacaaggccg tgagcgtgag ccggcggcgc gagctcagcg accgcgagct 15180 gatgcacagc ctgcaaaggg ccctggctgg cacgggcagc ggcgatagag aggccgagtc 15240 ctactttgac gcgggcgctg acctgcgctg ggccccaagc cgacgcgccc tggaggcagc 15300 tggggccgga cctgggctgg cggtggcacc cgcgcgcgct ggcaacgtcg gcggcgtgga 15360 ggaatatgac gaggacgatg agtacgagcc agaggacggc gagtactaag cggtgatgtt 15420 tctgatcaga tgatgcaaga cgcaacggac ccggcggtgc gggcggcgct gcagagccag 15480 ccgtccggcc ttaactccac ggacgactgg cgccaggtca tggaccgcat catgtcgctg 15540 actgcgcgca atcctgacgc gttccggcag cagccgcagg ccaaccggct ctccgcaatt 15600 ctggaagcgg tggtcccggc gcgcgcaaac cccacgcacg agaaggtgct ggcgatcgta 15660 aacgcgctgg ccgaaaacag ggccatccgg cccgacgagg ccggcctggt ctacgacgcg 15720 ctgcttcagc gcgtggctcg ttacaacagc ggcaacgtgc agaccaacct ggaccggctg 15780 gtgggggatg tgcgcgaggc cgtggcgcag cgtgagcgcg cgcagcagca gggcaacctg 15840 ggctccatgg ttgcactaaa cgccttcctg agtacacagc ccgccaacgt gccgcgggga 15900 caggaggact acaccaactt tgtgagcgca ctgcggctaa tggtgactga gacaccgcaa 15960 agtgaggtgt accagtctgg gccagactat tttttccaga ccagtagaca aggcctgcag 16020 accgtaaacc tgagccaggc tttcaaaaac ttgcaggggc tgtggggggt gcgggctccc 16080 acaggcgacc gcgcgaccgt gtctagcttg ctgacgccca actcgcgcct gttgctgctg 16140 ctaatagcgc ccttcacgga cagtggcagc gtgtcccggg acacatacct aggtcacttg 16200 ctgacactgt accgcgaggc cataggtcag gcgcatgtgg acgagcatac tttccaggag 16260 attacaagtg tcagccgcgc gctggggcag gaggacacgg gcagcctgga ggcaacccta 16320 aactacctgc tgaccaaccg gcggcagaag atcccctcgt tgcacagttt aaacagcgag 16380 gaggagcgca ttttgcgcta cgtgcagcag agcgtgagcc ttaacctgat gcgcgacggg 16440 gtaacgccca gcgtggcgct ggacatgacc gcgcgcaaca tggaaccggg catgtatgcc 16500 tcaaaccggc cgtttatcaa ccgcctaatg gactacttgc atcgcgcggc cgccgtgaac 16560 cccgagtatt tcaccaatgc catcttgaac ccgcactggc taccgccccc tggtttctac 16620 accgggggat tcgaggtgcc cgagggtaac gatggattcc tctgggacga catagacgac 16680 agcgtgtttt ccccgcaacc gcagaccctg ctagagttgc aacagcgcga gcaggcagag 16740 gcggcgctgc gaaaggaaag cttccgcagg ccaagcagct tgtccgatct aggcgctgcg 16800 gccccgcggt cagatgctag tagcccattt ccaagcttga tagggtctct taccagcact 16860 cgcaccaccc gcccgcgcct gctgggcgag gaggagtacc taaacaactc gctgctgcag 16920 ccgcagcgcg aaaaaaacct gcctccggca tttcccaaca acgggataga gagcctagtg 16980 gacaagatga gtagatggaa gacgtacgcg caggagcaca gggacgtgcc aggcccgcgc 17040 ccgcccaccc gtcgtcaaag gcacgaccgt cagcggggtc tggtgtggga ggacgatgac 17100 tcggcagacg acagcagcgt cctggatttg ggagggagtg gcaacccgtt tgcgcacctt 17160 cgccccaggc tggggagaat gttttaaaaa aaaaaaagca tgatgcaaaa taaaaaactc 17220 accaaggcca tggcaccgag cgttggtttt cttgtattcc ccttagtatg cggcgcgcgg 17280 cgatgtatga ggaaggtcct cctccctcct acgagagtgt ggtgagcgcg gcgccagtgg 17340 cggcggcgct gggttctccc ttcgatgctc ccctggaccc gccgtttgtg cctccgcggt 17400 acctgcggcc taccgggggg agaaacagca tccgttactc tgagttggca cccctattcg 17460 acaccacccg tgtgtacctg gtggacaaca agtcaacgga tgtggcatcc ctgaactacc 17520 agaacgacca cagcaacttt ctgaccacgg tcattcaaaa caatgactac agcccggggg 17580 aggcaagcac acagaccatc aatcttgacg accggtcgca ctggggcggc gacctgaaaa 17640 ccatcctgca taccaacatg ccaaatgtga acgagttcat gtttaccaat aagtttaagg 17700 cgcgggtgat ggtgtcgcgc ttgcctacta aggacaatca ggtggagctg aaatacgagt 17760 gggtggagtt cacgctgccc gagggcaact actccgagac catgaccata gaccttatga 17820 acaacgcgat cgtggagcac tacttgaaag tgggcagaca gaacggggtt ctggaaagcg 17880 acatcggggt aaagtttgac acccgcaact tcagactggg gtttgacccc gtcactggtc 17940 ttgtcatgcc tggggtatat acaaacgaag ccttccatcc agacatcatt ttgctgccag 18000 gatgcggggt ggacttcacc cacagccgcc tgagcaactt gttgggcatc cgcaagcggc 18060 aacccttcca ggagggcttt aggatcacct acgatgatct ggagggtggt aacattcccg 18120 cactgttgga tgtggacgcc taccaggcga gcttgaaaga tgacaccgaa cagggcgggg 18180 gtggcgcagg cggcagcaac agcagtggca gcggcgcgga agagaactcc aacgcggcag 18240 ccgcggcaat gcagccggtg gaggacatga acgatcatgc cattcgcggc gacacctttg 18300 ccacacgggc tgaggagaag cgcgctgagg ccgaagcagc ggccgaagct gccgcccccg 18360 ctgcgcaacc cgaggtcgag aagcctcaga agaaaccggt gatcaaaccc ctgacagagg 18420 acagcaagaa acgcagttac aacctaataa gcaatgacag caccttcacc cagtaccgca 18480 gctggtacct tgcatacaac tacggcgacc ctcagaccgg aatccgctca tggaccctgc 18540 tttgcactcc tgacgtaacc tgcggctcgg agcaggtcta ctggtcgttg ccagacatga 18600 tgcaagaccc cgtgaccttc cgctccacgc gccagatcag caactttccg gtggtgggcg 18660 ccgagctgtt gcccgtgcac tccaagagct tctacaacga ccaggccgtc tactcccaac 18720 tcatccgcca gtttacctct ctgacccacg tgttcaatcg ctttcccgag aaccagattt 18780 tggcgcgccc gccagccccc accatcacca ccgtcagtga aaacgttcct gctctcacag 18840 atcacgggac gctaccgctg cgcaacagca tcggaggagt ccagcgagtg accattactg 18900 acgccagacg ccgcacctgc ccctacgttt acaaggccct gggcatagtc tcgccgcgcg 18960 tcctatcgag ccgcactttt tgagcaagca tgtccatcct tatatcgccc agcaataaca 19020 caggctgggg cctgcgcttc ccaagcaaga tgtttggcgg ggccaagaag cgctccgacc 19080 aacacccagt gcgcgtgcgc gggcactacc gcgcgccctg gggcgcgcac aaacgcggcc 19140 gcactgggcg caccaccgtc gatgacgcca tcgacgcggt ggtggaggag gcgcgcaact 19200 acacgcccac gccgccacca gtgtccacag tggacgcggc cattcagacc gtggtgcgcg 19260 gagcccggcg ctatgctaaa atgaagagac ggcggaggcg cgtagcacgt cgccaccgcc 19320 gccgacccgg cactgccgcc caacgcgcgg cggcggccct gcttaaccgc gcacgtcgca 19380 ccggccgacg ggcggccatg cgggccgctc gaaggctggc cgcgggtatt gtcactgtgc 19440 cccccaggtc caggcgacga gcggccgccg cagcagccgc ggccattagt gctatgactc 19500 agggtcgcag gggcaacgtg tattgggtgc gcgactcggt tagcggcctg cgcgtgcccg 19560 tgcgcacccg ccccccgcgc aactagattg caagaaaaaa ctacttagac tcgtactgtt 19620 gtatgtatcc agcggcggcg gcgcgcaacg aagctatgtc caagcgcaaa atcaaagaag 19680 agatgctcca ggtcatcgcg ccggagatct atggcccccc gaagaaggaa gagcaggatt 19740 acaagccccg aaagctaaag cgggtcaaaa agaaaaagaa agatgatgat gatgaacttg 19800 acgacgaggt ggaactgctg cacgctaccg cgcccaggcg acgggtacag tggaaaggtc 19860 gacgcgtaaa acgtgttttg cgacccggca ccaccgtagt ctttacgccc ggtgagcgct 19920 ccacccgcac ctacaagcgc gtgtatgatg aggtgtacgg cgacgaggac ctgcttgagc 19980 aggccaacga gcgcctcggg gagtttgcct acggaaagcg gcataaggac atgctggcgt 20040 tgccgctgga cgagggcaac ccaacaccta gcctaaagcc cgtaacactg cagcaggtgc 20100 tgcccgcgct tgcaccgtcc gaagaaaagc gcggcctaaa gcgcgagtct ggtgacttgg 20160 cacccaccgt gcagctgatg gtacccaagc gccagcgact ggaagatgtc ttggaaaaaa 20220 tgaccgtgga acctgggctg gagcccgagg tccgcgtgcg gccaatcaag caggtggcgc 20280 cgggactggg cgtgcagacc gtggacgttc agatacccac taccagtagc accagtattg 20340 ccaccgccac agagggcatg gagacacaaa cgtccccggt tgcctcagcg gtggcggatg 20400 ccgcggtgca ggcggtcgct gcggccgcgt ccaagacctc tacggaggtg caaacggacc 20460 cgtggatgtt tcgcgtttca gccccccggc gcccgcgccg ttcgaggaag tacggcgccg 20520 ccagcgcgct actgcccgaa tatgccctac atccttccat tgcgcctacc cccggctatc 20580 gtggctacac ctaccgcccc agaagacgag caactacccg acgccgaacc accactggaa 20640 cccgccgccg ccgtcgccgt cgccagcccg tgctggcccc gatttccgtg cgcagggtgg 20700 ctcgcgaagg aggcaggacc ctggtgctgc caacagcgcg ctaccacccc agcatcgttt 20760 aaaagccggt ctttgtggtt cttgcagata tggccctcac ctgccgcctc cgtttcccgg 20820 tgccgggatt ccgaggaaga atgcaccgta ggaggggcat ggccggccac ggcctgacgg 20880 gcggcatgcg tcgtgcgcac caccggcggc ggcgcgcgtc gcaccgtcgc atgcgcggcg 20940 gtatcctgcc cctccttatt ccactgatcg ccgcggcgat tggcgccgtg cccggaattg 21000 catccgtggc cttgcaggcg cagagacact gattaaaaac aagttgcatg tggaaaaatc 21060 aaaataaaaa gtctggactc tcacgctcgc ttggtcctgt aactattttg tagaatggaa 21120 gacatcaact ttgcgtctct ggccccgcga cacggctcgc gcccgttcat gggaaactgg 21180 caagatatcg gcaccagcaa tatgagcggt ggcgccttca gctggggctc gctgtggagc 21240 ggcattaaaa atttcggttc caccgttaag aactatggca gcaaggcctg gaacagcagc 21300 acaggccaga tgctgaggga taagttgaaa gagcaaaatt tccaacaaaa ggtggtagat 21360 ggcctggcct ctggcattag cggggtggtg gacctggcca accaggcagt gcaaaataag 21420 attaacagta agcttgatcc ccgccctccc gtagaggagc ctccaccggc cgtggagaca 21480 gtgtctccag aggggcgtgg cgaaaagcgt ccgcgccccg acagggaaga aactctggtg 21540 acgcaaatag acgagcctcc ctcgtacgag gaggcactaa agcaaggcct gcccaccacc 21600 cgtcccatcg cgcccatggc taccggagtg ctgggccagc acacacccgt aacgctggac 21660 ctgcctcccc ccgccgacac ccagcagaaa cctgtgctgc caggcccgac cgccgttgtt 21720 gtaacccgtc ctagccgcgc gtccctgcgc cgcgccgcca gcggtccgcg atcgttgcgg 21780 cccgtagcca gtggcaactg gcaaagcaca ctgaacagca tcgtgggtct gggggtgcaa 21840 tccctgaagc gccgacgatg cttctgatag ctaacgtgtc gtatgtgtgt catgtatgcg 21900 tccatgtcgc cgccagagga gctgctgagc cgccgcgcgc ccgctttcca agatggctac 21960 cccttcgatg atgccgcagt ggtcttacat gcacatctcg ggccaggacg cctcggagta 22020 cctgagcccc gggctggtgc agtttgcccg cgccaccgag acgtacttca gcctgaataa 22080 caagtttaga aaccccacgg tggcgcctac gcacgacgtg accacagacc ggtcccagcg 22140 tttgacgctg cggttcatcc ctgtggaccg tgaggatact gcgtactcgt acaaggcgcg 22200 gttcacccta gctgtgggtg ataaccgtgt gctggacatg gcttccacgt actttgacat 22260 ccgcggcgtg ctggacaggg gccctacttt taagccctac tctggcactg cctacaacgc 22320 cctggctccc aagggtgccc caaatccttg cgaatgggat gaagctgcta ctgctcttga 22380 aataaaccta gaagaagagg acgatgacaa cgaagacgaa gtagacgagc aagctgagca 22440 gcaaaaaact cacgtatttg ggcaggcgcc ttattctggt ataaatatta caaaggaggg 22500 tattcaaata ggtgtcgaag gtcaaacacc taaatatgcc gataaaacat ttcaacctga 22560 acctcaaata ggagaatctc agtggtacga aacagaaatt aatcatgcag ctgggagagt 22620 cctaaaaaag actaccccaa tgaaaccatg ttacggttca tatgcaaaac ccacaaatga 22680 aaatggaggg caaggcattc ttgtaaagca acaaaatgga aagctagaaa gtcaagtgga 22740 aatgcaattt ttctcaacta ctgaggcagc cgcaggcaat ggtgataact tgactcctaa 22800 agtggtattg tacagtgaag atgtagatat agaaacccca gacactcata tttcttacat 22860 gcccactatt aaggaaggta actcacgaga actaatgggc caacaatcta tgcccaacag 22920 gcctaattac attgctttta gggacaattt tattggtcta atgtattaca acagcacggg 22980 taatatgggt gttctggcgg gccaagcatc gcagttgaat gctgttgtag atttgcaaga 23040 cagaaacaca gagctttcat accagctttt gcttgattcc attggtgata gaaccaggta 23100 cttttctatg tggaatcagg ctgttgacag ctatgatcca gatgttagaa ttattgaaaa 23160 tcatggaact gaagatgaac ttccaaatta ctgctttcca ctgggaggtg tgattaatac 23220 agagactctt accaaggtaa aacctaaaac aggtcaggaa aatggatggg aaaaagatgc 23280 tacagaattt tcagataaaa atgaaataag agttggaaat aattttgcca tggaaatcaa 23340 tctaaatgcc aacctgtgga gaaatttcct gtactccaac atagcgctgt atttgcccga 23400 caagctaaag tacagtcctt ccaacgtaaa aatttctgat aacccaaaca cctacgacta 23460 catgaacaag cgagtggtgg ctcccgggct agtggactgc tacattaacc ttggagcacg 23520 ctggtccctt gactatatgg acaacgtcaa cccatttaac caccaccgca atgctggcct 23580 gcgctaccgc tcaatgttgc tgggcaatgg tcgctatgtg cccttccaca tccaggtgcc 23640 tcagaagttc tttgccatta aaaacctcct tctcctgccg ggctcataca cctacgagtg 23700 gaacttcagg aaggatgtta acatggttct gcagagctcc ctaggaaatg acctaagggt 23760 tgacggagcc agcattaagt ttgatagcat ttgcctttac gccaccttct tccccatggc 23820 ccacaacacc gcctccacgc ttgaggccat gcttagaaac gacaccaacg accagtcctt 23880 taacgactat ctctccgccg ccaacatgct ctaccctata cccgccaacg ctaccaacgt 23940 gcccatatcc atcccctccc gcaactgggc ggctttccgc ggctgggcct tcacgcgcct 24000 taagactaag gaaaccccat cactgggctc gggctacgac ccttattaca cctactctgg 24060 ctctataccc tacctagatg gaacctttta cctcaaccac acctttaaga aggtggccat 24120 tacctttgac tcttctgtca gctggcctgg caatgaccgc ctgcttaccc ccaacgagtt 24180 tgaaattaag cgctcagttg acggggaggg ttacaacgtt gcccagtgta acatgaccaa 24240 agactggttc ctggtacaaa tgctagctaa ctataacatt ggctaccagg gcttctatat 24300 cccagagagc tacaaggacc gcatgtactc cttctttaga aacttccagc ccatgagccg 24360 tcaggtggtg gatgatacta aatacaagga ctaccaacag gtgggcatcc tacaccaaca 24420 caacaactct ggatttgttg gctaccttgc ccccaccatg cgcgaaggac aggcctaccc 24480 tgctaacttc ccctatccgc ttataggcaa gaccgcagtt gacagcatta cccagaaaaa 24540 gtttctttgc gatcgcaccc tttggcgcat cccattctcc agtaacttta tgtccatggg 24600 cgcactcaca gacctgggcc aaaaccttct ctacgccaac tccgcccacg cgctagacat 24660 gacttttgag gtggatccca tggacgagcc cacccttctt tatgttttgt ttgaagtctt 24720 tgacgtggtc cgtgtgcacc agccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac 24780 gcccttctcg gccggcaacg ccacaacata aagaagcaag caacatcaac aacagctgcc 24840 gccatgggct ccagtgagca ggaactgaaa gccattgtca aagatcttgg ttgtgggcca 24900 tattttttgg gcacctatga caagcgcttt ccaggctttg tttctccaca caagctcgcc 24960 tgcgccatag tcaatacggc cggtcgcgag actgggggcg tacactggat ggcctttgcc 25020 tggaacccgc actcaaaaac atgctacctc tttgagccct ttggcttttc tgaccagcga 25080 ctcaagcagg tttaccagtt tgagtacgag tcactcctgc gccgtagcgc cattgcttct 25140 tcccccgacc gctgtataac gctggaaaag tccacccaaa gcgtacaggg gcccaactcg 25200 gccgcctgtg gactattctg ctgcatgttt ctccacgcct ttgccaactg gccccaaact 25260 cccatggatc acaaccccac catgaacctt attaccgggg tacccaactc catgctcaac 25320 agtccccagg tacagcccac cctgcgtcgc aaccaggaac agctctacag cttcctggag 25380 cgccactcgc cctacttccg cagccacagt gcgcagatta ggagcgccac ttctttttgt 25440 cacttgaaaa acatgtaaaa ataatgtact agagacactt tcaataaagg caaatgcttt 25500 tatttgtaca ctctcgggtg attatttacc cccacccttg ccgtctgcgc cgtttaaaaa 25560 tcaaaggggt tctgccgcgc atcgctatgc gccactggca gggacacgtt gcgatactgg 25620 tgtttagtgc tccacttaaa ctcaggcaca accatccgcg gcagctcggt gaagttttca 25680 ctccacaggc tgcgcaccat caccaacgcg tttagcaggt cgggcgccga tatcttgaag 25740 tcgcagttgg ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg 25800 aacactatca gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc 25860 gcgtccaggt cctccgcgtt gctcagggcg aacggagtca actttggtag ctgccttccc 25920 aaaaagggcg cgtgcccagg ctttgagttg cactcgcacc gtagtggcat caaaaggtga 25980 ccgtgcccgg tctgggcgtt aggatacagc gcctgcataa aagccttgat ctgcttaaaa 26040 gccacctgag cctttgcgcc ttcagagaag aacatgccgc aagacttgcc ggaaaactga 26100 ttggccggac aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga gatctgcacc 26160 acatttcggc cccaccggtt cttcacgatc ttggccttgc tagactgctc cttcagcgcg 26220 cgctgcccgt tttcgctcgt cacatccatt tcaatcacgt gctccttatt tatcataatg 26280 cttccgtgta gacacttaag ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg 26340 cagcccgtgg gctcgtgatg cttgtaggtc acctctgcaa acgactgcag gtacgcctgc 26400 aggaatcgcc ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg 26460 cggtgctcct cgttcagcca ggtcttgcat acggccgcca gagcttccac ttggtcaggc 26520 agtagtttga agttcgcctt tagatcgtta tccacgtggt acttgtccat cagcgcgcgc 26580 gcagcctcca tgcccttctc ccacgcagac acgatcggca cactcagcgg gttcatcacc 26640 gtaatttcac tttccgcttc gctgggctct tcctcttcct cttgcgtccg cataccacgc 26700 gccactgggt cgtcttcatt cagccgccgc actgtgcgct tacctccttt gccatgcttg 26760 attagcaccg gtgggttgct gaaacccacc atttgtagcg ccacatcttc tctttcttcc 26820 tcgctgtcca cgattacctc tggtgatggc gggcgctcgg gcttgggaga agggcgcttc 26880 tttttcttct tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg cgggctgggt 26940 gtgcgcggca ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc gatacgccgc 27000 ctcatccgct tttttggggg cgcccgggga ggcggcggcg acggggacgg ggacgacacg 27060 tcctccatgg ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc 27120 tgctcctctt cccgactggc catttccttc tcctataggc agaaaaagat catggagtca 27180 gtcgagaaga aggacagcct aaccgccccc tctgagttcg ccaccaccgc ctccaccgat 27240 gccgccaacg cgcctaccac cttccccgtc gaggcacccc cgcttgagga ggaggaagtg 27300 attatcgagc aggacccagg ttttgtaagc gaagacgacg aggaccgctc agtaccaaca 27360 gaggataaaa agcaagacca ggacaacgca gaggcaaacg aggaacaagt cgggcggggg 27420 gacgaaaggc atggcgacta cctagatgtg ggagacgacg tgctgttgaa gcatctgcag 27480 cgccagtgcg ccattatctg cgacgcgttg caagagcgca gcgatgtgcc cctcgccata 27540 gcggatgtca gccttgccta cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc 27600 caagaaaacg gcacatgcga gcccaacccg cgcctcaact tctaccccgt atttgccgtg 27660 ccagaggtgc ttgccaccta tcacatcttt ttccaaaact gcaagatacc cctatcctgc 27720 cgtgccaacc gcagccgagc ggacaagcag ctggccttgc ggcagggcgc tgtcatacct 27780 gatatcgcct cgctcaacga agtgccaaaa atctttgagg gtcttggacg cgacgagaag 27840 cgcgcggcaa acgctctgca acaggaaaac agcgaaaatg aaagtcactc tggagtgttg 27900 gtggaactcg agggtgacaa cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc 27960 cactttgcct acccggcact taacctaccc cccaaggtca tgagcacagt catgagtgag 28020 ctgatcgtgc gccgtgcgca gcccctggag agggatgcaa atttgcaaga acaaacagag 28080 gagggcctac ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct 28140 gccgacttgg aggagcgacg caaactaatg atggccgcag tgctcgttac cgtggagctt 28200 gagtgcatgc agcggttctt tgctgacccg gagatgcagc gcaagctaga ggaaacattg 28260 cactacacct ttcgacaggg ctacgtacgc caggcctgca agatctccaa cgtggagctc 28320 tgcaacctgg tctcctacct tggaattttg cacgaaaacc gccttgggca aaacgtgctt 28380 cattccacgc tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt 28440 ctatgctaca cctggcagac ggccatgggc gtttggcagc agtgcttgga ggagtgcaac 28500 ctcaaggagc tgcagaaact gctaaagcaa aacttgaagg acctatggac ggccttcaac 28560 gagcgctccg tggccgcgca cctggcggac atcattttcc ccgaacgcct gcttaaaacc 28620 ctgcaacagg gtctgccaga cttcaccagt caaagcatgt tgcagaactt taggaacttt 28680 atcctagagc gctcaggaat cttgcccgcc acctgctgtg cacttcctag cgactttgtg 28740 cccattaagt accgcgaatg ccctccgccg ctttggggcc actgctacct tctgcagcta 28800 gccaactacc ttgcctacca ctctgacata atggaagacg tgagcggtga cggtctactg 28860 gagtgtcact gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg caattcgcag 28920 ctgcttaacg aaagtcaaat tatcggtacc tttgagctgc agggtccctc gcctgacgaa 28980 aagtccgcgg ctccggggtt gaaactcact ccggggctgt ggacgtcggc ttaccttcgc 29040 aaatttgtac ctgaggacta ccacgcccac gagattaggt tctacgaaga ccaatcccgc 29100 ccgcctaatg cggagcttac cgcctgcgtc attacccagg gccacattct tggccaattg 29160 caagccatca acaaagcccg ccaagagttt ctgctacgaa agggacgggg ggtttacttg 29220 gacccccagt ccggcgagga gctcaaccca atccccccgc cgccgcagcc ctatcagcag 29280 cagccgcggg cccttgcttc ccaggatggc acccaaaaag aagctgcagc tgccgccgcc 29340 acccacggac gaggaggaat actgggacag tcaggcagag gaggttttgg acgaggagga 29400 ggaggacatg atggaagact gggagagcct agacgaggaa gcttccgagg tcgaagaggt 29460 gtcagacgaa acaccgtcac cctcggtcgc attcccctcg ccggcgcccc agaaatcggc 29520 aaccggttcc agcatggcta caacctccgc tcctcaggcg ccgccggcac tgcccgttcg 29580 ccgacccaac cgtagatggg acaccactgg aaccagggcc ggtaagtcca agcagccgcc 29640 gccgttagcc caagagcaac aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa 29700 cgccatagtt gcttgcttgc aagactgtgg gggcaacatc tccttcgccc gccgctttct 29760 tctctaccat cacggcgtgg ccttcccccg taacatcctg cattactacc gtcatctcta 29820 cagcccatac tgcaccggcg gcagcggcag caacagcagc ggccacacag aagcaaaggc 29880 gaccggatag caagactctg acaaagccca agaaatccac agcggcggca gcagcaggag 29940 gaggagcgct gcgtctggcg cccaacgaac ccgtatcgac ccgcgagctt agaaacagga 30000 tttttcccac tctgtatgct atatttcaac agagcagggg ccaagaacaa gagctgaaaa 30060 taaaaaacag gtctctgcga tccctcaccc gcagctgcct gtatcacaaa agcgaagatc 30120 agcttcggcg cacgctggaa gacgcggagg ctctcttcag taaatactgc gcgctgactc 30180 ttaaggacta gtttcgcgcc ctttctcaaa tttaagcgcg aaaactacgt catctccagc 30240 ggccacaccc ggcgccagca cctgttgtca gcgccattat gagcaaggaa attcccacgc 30300 cctacatgtg gagttaccag ccacaaatgg gacttgcggc tggagctgcc caagactact 30360 caacccgaat aaactacatg agcgcgggac cccacatgat atcccgggtc aacggaatac 30420 gcgcccaccg aaaccgaatt ctcctggaac aggcggctat taccaccaca cctcgtaata 30480 accttaatcc ccgtagttgg cccgctgccc tggtgtacca ggaaagtccc gctcccacca 30540 ctgtggtact tcccagagac gcccaggccg aagttcagat gactaactca ggggcgcagc 30600 ttgcgggcgg ctttcgtcac agggtgcggt cgcccgggca gggtataact cacctgacaa 30660 tcagagggcg aggtattcag ctcaacgacg agtcggtgag ctcctcgctt ggtctccgtc 30720 cggacgggac atttcagatc ggcggcgccg gccgctcttc attcacgcct cgtcaggcaa 30780 tcctaactct gcagacctcg tcctctgagc cgcgctctgg aggcattgga actctgcaat 30840 ttattgagga gtttgtgcca tcggtctact ttaacccctt ctcgggacct cccggccact 30900 atccggatca atttattcct aactttgacg cggtaaagga ctcggcggac ggctacgact 30960 gaatgttaag tggagaggca gagcaactgc gcctgaaaca cctggtccac tgtcgccgcc 31020 acaagtgctt tgcccgcgac tccggtgagt tttgctactt tgaattgccc gaggatcata 31080 tcgagggccc ggcgcacggc gtccggctta ccgcccaggg agagcttgcc cgtagcctga 31140 ttcgggagtt tacccagcgc cccctgctag ttgagcggga caggggaccc tgtgttctca 31200 ctgtgatttg caactgtcct aaccctggat tacatcaaga tcctctagtt aatgtcaggt 31260 cgcctaagtc gattaactag agtacccggg gatcttattc cctttaacta ataaaaaaaa 31320 ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31380 cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31440 ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31500 catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31560 atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31620 caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 31680 tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 31740 caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 31800 cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 31860 cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 31920 cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 31980 gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32040 tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32100 ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32160 cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32220 aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32280 aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32340 tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32400 ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32460 caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32520 agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32580 cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 32640 actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 32700 tgataagcta actttgtgga ccggaataaa ccctccacct aactgtcaaa ttgtggaaaa 32760 cactaataca aatgatggca aacttacttt agtattagta aaaaatggag ggcttgttaa 32820 tggctacgtg tctctagttg gtgtatcaga cactgtgaac caaatgttca cacaaaagac 32880 agcaaacatc caattaagat tatattttga ctcttctgga aatctattaa ctgaggaatc 32940 agacttaaaa attccactta aaaataaatc ttctacagcg accagtgaaa ctgtagccag 33000 cagcaaagcc tttatgccaa gtactacagc ttatcccttc aacaccacta ctagggatag 33060 tgaaaactac attcatggaa tatgttacta catgactagt tatgatagaa gtctatttcc 33120 cttgaacatt tctataatgc taaacagccg tatgatttct tccaatgttg cctatgccat 33180 acaatttgaa tggaatctaa atgcaagtga atctccagaa agcaacatag ctacgctgac 33240 cacatccccc tttttctttt cttacattac agaagacgac aactaaagaa tcgtttgtgt 33300 tatgtttcaa cgtgtttatt tttcaattgc agaaaatttc aagtcatttt tcattcagta 33360 gtatagcccc accaccacat agcttataca gatcaccgta ccttaatcaa actcacagaa 33420 ccctagtatt caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc 33480 ggctggcctt aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc 33540 acacggtttc ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct 33600 cacttaagtt catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt 33660 gcttaacggg cggcgaagga gaagtccacg cgttgtgcat tgtcaaagtg ttacattcgg 33720 gcagcagcgg atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac 33780 gatccctact gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc 33840 caaatggaac gccggacgta gtcatatttc ctgaagcaaa accaggtgcg ggcgtgacaa 33900 acagatctgc gtctccggtc tcgccgctta gatcgctctg tgtagtagtt gtagtatatc 33960 cactctctca aagcatccag gcgccccctg gcttcgggtt ctatgtaaac tccttcatgc 34020 gccgctgccc tgataacatc caccaccgca gaataagcca cacccagcca acctacacat 34080 tcgttctgcg agtcacacac gggaggagcg ggaagagctg gaagaaccat gttttttttt 34140 ttattccaaa agattatcca aaacctcaaa atgaagatct attaagtgaa cgcgctcccc 34200 tccggtggcg tggtcaaact ctacagccaa agaacagata atggcatttg taagatgttg 34260 cacaatggct tccaaaaggc aaacggccct cacgtccaag tggacgtaaa ggctaaaccc 34320 ttcagggtga atctcctcta taaacattcc agcaccttca accatgccca aataattctc 34380 atctcgccac cttctcaata tatctctaag caaatcccga atattaagtc cggccattgt 34440 aaaaatctgc tccagagcgc cctccacctt cagcctcaag cagcgaatca tgattgcaaa 34500 aattcaggtt cctcacagac ctgtataaga ttcaaaagcg gaacattaac aaaaataccg 34560 cgatcccgta ggtcccttcg cagggccagc tgaacataat cgtgcaggtc tgcacggacc 34620 agcgcggcca cttccccgcc aggaaccatg acaaaagaac ccacactgat tatgacacgc 34680 atactcggag ctatgctaac cagcgtagcc ccgatgtaag cttgttgcat gggcggcgat 34740 ataaaatgca aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa agaaagcaca 34800 tcgtagtcat gctcatgcag ataaaggcag gtaagctccg gaaccaccac agaaaaagac 34860 accatttttc tctcaaacat gtctgcgggt ttctgcataa acacaaaata aaataacaaa 34920 aaaacattta aacattagaa gcctgtctta caacaggaaa aacaaccctt ataagcataa 34980 gacggactac ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca 35040 ccaccgacag ctcctcggtc atgtccggag tcataatgta agactcggta aacacatcag 35100 gttgattcac atcggtcagt gctaaaaagc gaccgaaata gcccggggga atacataccc 35160 gcaggcgtag agacaacatt acagccccca taggaggtat aacaaaatta ataggagaga 35220 aaaacacata aacacctgaa aaaccctcct gcctaggcaa aatagcaccc tcccgctcca 35280 gaacaacata cagcgcttcc acagcggcag ccataacagt cagccttacc agtaaaaaag 35340 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa 35400 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc 35460 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc 35520 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtcacttcc cattttaaga 35580 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg 35640 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc 35700 caaaataagg tatattattg atgatg 35726

Claims (19)

1. 코로나바이러스 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질.
   
2. 제1항에 있어서, 상기 코로나바이러스는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 하는, 단백질.
   
3. 제2항에 있어서, 상기 코로나바이러스는 SARS-CoV-2인 것을 특징으로 하는, 단백질.
   
4. 제1항에 있어서, 상기 링커는 (GGGGS)n으로 구성되는 것을 특징으로 하는, 단백질.
   
5. 제4항에 있어서, 상기 n은 1 내지 5의 정수인 것을 특징으로 하는, 단백질.
   
6. 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 링커 서열로 연결된 스파이크 단백질 항원은 안정성(stability)이 증가된 것을 특징으로 하는, 단백질.
   
7. 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 링커 서열로 연결된 스파이크 단백질 항원은 항원 발현도가 증가하는 것을 특징으로 하는, 단백질.
   
8. 코로나바이러스 스파이크 단백질(Spike protein)의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드.
   
9. 제8항에 있어서, 상기 코로나바이러스는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 하는, 폴리뉴클레오티드.
   
10. 제8항에 있어서, 상기 링커 서열은 (GGGGS)n으로 구성되는 것을 특징으로 하는, 폴리뉴클레오티드.
    
11. 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자 사이의 절단 인지 서열이 제거되고 링커 서열을 도입한 것을 특징으로 하는, 재조합 스파이크 단백질을 코딩하는 폴리뉴클레오티드를 포함하는 벡터.
    
12. 제11항에 있어서, 상기 코로나바이러스는 SARS-CoV, MERS-CoV 또는 SARS-CoV-2인 것을 특징으로 하는, 벡터.
    
13. 제12항에 있어서, 상기 코로나바이러스는 SARS-CoV-2인 것을 특징으로 하는, 벡터.
    
14. 제11항에 있어서, 상기 벡터는 바이러스 또는 플라스미드인 것을 특징으로 하는, 벡터.
    
15. 제14항에 있어서, 상기 바이러스는 아데노바이러스, 레트로바이러스, 렌티바이러스, modified vaccinia virus ankara(MVA), 아데노 연관 바이러스(Adeno-associated virus, AAV), 단순 헤르페스 바이러스(Herpes simplex virus) 또는 배큘로 바이러스(Baculovirus) 인 것을 특징으로 하는, 벡터.
    
16. 제15항에 있어서, 상기 아데노바이러스는 Ad2, Ad4, Ad5, Ad11, Ad26, Ad35, ChAd68, FAd9 또는 PAd3인 것을 특징으로 하는, 벡터.
    
17. 제15항 또는 제16항에 있어서, 상기 아데노바이러스는 항원 발현도가 높은 것을 특징으로 하는, 벡터.
    
18. 코로나바이러스 스파이크 단백질의 S1 및 S2 유전자의 폴리뉴클레오티드를 포함하는 벡터를 포함하는 코로나바이러스 감염증 예방용 또는 치료용 백신.
    
19. 제18항에 있어서, 상기 코로나바이러스 감염증은 SARS, MERS 또는 COVID-19인 것을 특징으로 하는, 코로나바이러스 감염증 예방용 또는 치료용 백신.
    

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