KR20210029359A - Cosmetic composition for improving acne - Google Patents

Abstract

The present invention relates to a cosmetic composition, which specifically comprises dipotassium glycyrrhizate in an amount of 0.01 to 0.07 wt%, camellia oil in an amount of 0.01 to 0.4 wt%, an Epilobium fleischeri extract in an amount of 0.05 to 0.25 wt%, a witch hazel extract in an amount of 0.1 to 2 wt%, a willow bark extract in an amount of 0.1 to 0.7 wt%, and a chamomile extract in an amount of 0.1 to 0.4 wt%. The composition of the present invention has excellent skin soothing and acne alleviating effects, and excellent moisturizing and elasticity improvement effects, thereby contributing to the cosmetic industry.

Description

Cosmetic composition for improving acne {COSMETIC COMPOSITION FOR IMPROVING ACNE}

The present invention relates to a cosmetic composition.

Acne refers to the inflammation of many hair follicles on the faces, especially cheeks and foreheads of adolescent men and women. Sometimes it develops on the chest or back, and sometimes it occurs around the age of 40. Initially, the hair follicles are filled with cortex and dead skin cells, forming a yellowish-white lump, and sometimes the apex may turn black. At this time, if you squeeze it, you will get a cheese shape. Then, it becomes inflamed and raised red around it. When bacteria are infected with it, it becomes purulent and yellow. Crater-shaped traces remain on the better spot. Usually these four kinds of things are mixed. The cause is often caused by bacterial infection in sebum secreted excessively by the action of congenital traits and sex hormones, especially male hormones. In women after the age of 22, it often worsens just before menstruation. Although the cause of acne is not yet clearly identified, it can be summarized as an increase in sebum secretion, skin keratinization and peroxidation of lipid components, proliferation of acne causative bacteria, and inducing and deteriorating inflammation. One of the important features associated with the onset of acne is an increase in sebum secretion, which is known to be closely related to androgens, a male hormone. As male hormones are secreted above normal levels, the function of the sebaceous glands is promoted, and the resulting excess sebum is stagnated in the hair follicle due to the keratinization of the hair follicle wall, creating a comedones. The sebum accumulated in the hair follicle that cannot be discharged to the outside blocks the pores and prevents the inflow of external air, so that the inside of the hair follicle is an environment in which propionibacterium acnes, an anaerobic bacteria, can proliferate smoothly. Propionibacterium acnes secretes lipolytic enzymes and chemical factors to make free fatty acids, triggers an immune reaction of leukocytes and destroys the hair follicle wall, causing the contents to flow into the dermis, resulting in an inflammatory reaction. . Therefore, the general treatment method for acne can be summarized in four cases: inhibition of excessive sebum production, inhibition of keratinization of the hair follicle wall, inhibition of proliferation of propionibacterium acnes, and minimization or inhibition of inflammatory reactions. .

Various microorganisms exist in human skin. These microorganisms cause various skin diseases. Staphylococcus aureus, which causes purulent in the skin, as well as Staphylococcus epidermis, and Propionibacterium acnes, as the skin flora, and these bacteria are used to absorb sebum or sweat secreted from the skin. It decomposes to generate odor, and in addition, decomposition products irritate the skin and cause inflammation. In particular, Propionibacterium acnes decomposes sebum using lipase, a fatty acid degrading enzyme, to produce free fatty acids. These free fatty acids not only irritate the skin, but also cause inflammatory lesions such as papules, pustules, and nodules, which are red rashes found in acne.

In the area of acne treatment, various medicinal materials have been developed and used, but the selection of cosmetics for proper skin care has not been studied yet. In the cosmetic field, it is a concept that heals acne by removing hair follicle stenosis through skin peeling, mainly with soap, medicinal soap, and an abrasive cleanser. In addition, the skin peeling and sterilizing effect of acne bacteria by the alcohol component of Astrigent is also effective against acne to some extent. There are also cosmetics containing resorcinol, sulfur, salicylic acid, and benzoyl peroxide. However, since these ingredients are irritating to the skin, there are many side effects and limitations, such as limiting the concentration to be used.

In addition, antibiotics such as erythromycine for removing acne bacteria, anti-inflamation raw materials for inflammation treatment, and hormone preparations for sebum control are used, but these are mainly raw materials in the pharmaceutical field.

Until now, products have been developed focusing on only one of the aforementioned various mechanisms of occurrence of acne, so the healing effect is insignificant and there is a problem that recurrence is frequent. In addition, there is no preventive concept, and there is no product with a healing concept in the cosmetic field.

Korean Patent Registration No. 1749690

An object of the present invention is to provide a cosmetic composition having skin soothing and acne improvement effects.

1.Dipotassium Glycyrrhizate 0.01 to 0.07 wt%, Camellia Oil 0.01 to 0.4 wt%, Epilobium Fleischeri Extract 0.05 to 0.25 wt%, Witch Hazel extract ( Witch Hazel Extract) 0.1 to 2% by weight, willow bark extract (Willow Bark Extract) 0.1 to 0.7% by weight and chamomile extract (Camomile Extract) containing 0.1 to 0.4% by weight, cosmetic composition.

2. In the above 1, 0.02 to 0.1% by weight of vitamin B6/HCl, 0.1 to 1% by weight of niacin amide, 0.1 to 0.5% by weight of methyl sulfonyl methane (MSM), and salicylic acid ) 0.04 to 0.2% by weight, aloe leaf extract (Aloe Leaf Extract) 0.1 to 2% by weight and glycolic acid (Glycolic Acid) 0.04 to 0.2% by weight, further comprising a cosmetic composition.

3. In the above 2, 0.1 to 0.4% by weight of a glucose and fructose mixture, 1 to 7% by weight of a mixture of Schisandra chinensis, ginger, yellow lotus and green tea extract, and 0.1 to 2% by weight of a mixture of Propolis and Portulaca Oleracea extract A cosmetic composition further comprising a.

4. In the above 1, the composition is for acne improvement, cosmetic composition.

The composition of the present invention is excellent in soothing skin and improving acne, and also has excellent moisturizing and elasticity improvement effects.

Hereinafter, the present invention will be described in detail.

The present invention is Dipotassium Glycyrrhizate (Dipotassium Glycyrrhizate) 0.01 to 0.07% by weight, Camellia Oil (Camellia Oil) 0.01 to 0.4% by weight, Epilobium Fleischeri Extract (Epilobium Fleischeri Extract) 0.05 to 0.25% by weight, Witch Hazel extract (Witch Hazel Extract) 0.1 to 2% by weight, willow bark extract (Willow Bark Extract) 0.1 to 0.7% by weight, and chamomile extract (Camomile Extract) 0.1 to 0.4% by weight, containing 0.1 to 0.4% by weight, relates to a cosmetic composition.

The cosmetic composition of the present invention not only has excellent moisturizing and elasticity improvement effects, but also has excellent skin soothing and acne improvement effects.

The dipotassium glycyrrhizate is for imparting a conditioning effect to the skin and alleviating irritation of the skin, and the content of the dipotassium glycyrrhizate is 0.01 to 0.07% by weight, preferably 0.03 to 0.05% by weight. %, more preferably 0.035 to 0.045% by weight. However, it is not limited thereto.

The camellia oil is to increase the flexibility of the composition and to improve trouble, and the content of the camellia oil is 0.01 to 0.4% by weight, preferably 0.1 to 0.3% by weight, more preferably 0.15 to 0.25% by weight. %, but is not limited thereto.

The Epilobium Playserry extract has an anti-inflammatory effect and is to increase metabolic function, and the content of the Epilobium Playserry Extract is 0.05 to 0.25% by weight, preferably 0.1 to 0.2% by weight. %, more preferably 0.13 to 0.17% by weight, but is not limited thereto.

The epilobium playscerry extract may be extracted from the leaves, roots and stems of the epilobium playscerry.

The term "extract" as used herein refers to a solvent containing, for example, ethanol, methanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixture thereof, regardless of the extraction method. It is understood as meaning including an extract obtained by extraction, and an extract fractionated from the extract with the solvents listed above. As long as the extraction target is extracted through the step of immersing the extraction solvent, it should be understood that any method such as cold sedimentation, reflux, warming, and ultrasonic waves can be applied. In one embodiment, the extract is obtained by extracting the object to be extracted with water, ethanol, or a mixed solvent thereof, and may mean including a concentrated liquid extract or solid extract from which the extraction solvent has been removed.

According to an embodiment of the present invention, the amount of the solvent relative to the content to be extracted of the "extract" may be 5 to 35 times, preferably 10 to 30 times, more preferably 15 to 25 times, and the concentration of the solvent is It may be 50 to 90% (v/v), preferably 60 to 80% (v/v), more preferably 65 to 75% (v/v), but is not limited thereto.

In addition, according to an embodiment of the present invention, the extraction method of the "extract" is hot water for 1 to 5 hours, preferably 2 to 4 hours, more preferably 2 hours 30 minutes to 3 hours 30 minutes in a heating extractor. It may be extracted and obtained by lyophilizing it, but is not limited thereto.

The temperature of the hot water may be, for example, 70 to 130°C, preferably 80 to 120°C, more preferably 90 to 110°C, but is not limited thereto.

The witch hazel extract is a substance causing an astringent action of the skin and controls sebum production, and may be extracted from the leaves, roots and stems of witch hazel. The content of the Witch Hazel Extract may be 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.2% by weight, but is not limited thereto.

The willow bark extract activates cells, can cure inflammation, and has an antibiotic effect, and the content of the willow bark extract is 0.1 to 0.7% by weight, preferably 0.2 to 0.6% by weight. %, more preferably 0.3 to 0.5% by weight, but is not limited thereto.

The chamomile extract has a soothing effect and an anti-inflammatory effect on the skin, and may be extracted from the leaves, stems, and roots of chamomile. The content of the chamomile extract may be 0.1 to 0.4% by weight, preferably 0.2 to 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

Each component included in the composition has excellent moisturizing and elasticity improvement effects within each content range, and further has excellent skin soothing and acne improvement effects.

According to an embodiment of the present invention, the composition of the present invention contains 0.02 to 0.1% by weight of vitamin B6/HCl, 0.1 to 1% by weight of niacin amide, and 0.1 to 0.5 of methyl sulfonyl methane (MSM). It may further include 0.04 to 0.2% by weight of salicylic acid, 0.1 to 2% by weight of Aloe Leaf Extract, and 0.04 to 0.2% by weight of glycolic acid.

The vitamin B6/HCl refers to the hydrochloride salt of vitamin B6, has an anti-inflammatory effect and trouble improving effect, and the content of vitamin B6/HCl is 0.02 to 0.1% by weight, preferably 0.04 to 0.08% by weight, more preferably May be 0.05 to 0.07% by weight, but is not limited thereto.

The niacin amide is for a whitening effect and an inflammation healing effect, and the content of the niacin amide may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight. However, it is not limited thereto.

The methyl sulfonyl methane is for removing natural sulfur-toxins, and the content of methyl sulfonyl methane (MSM) is 0.1 to 0.5% by weight, preferably 0.2 to 0.4% by weight, more preferably 0.25 It may be to 0.35% by weight, but is not limited thereto.

The salicylic acid is for keratin improvement and antibacterial effect, and the content of salicylic acid may be 0.04 to 0.2% by weight, preferably 0.07 to 0.17% by weight, more preferably 0.1 to 0.14% by weight. It is not limited.

The aloe leaf extract is a component for wound healing, moisturizing effect and erythema prevention, and the content of the aloe leaf extract is 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.9 It may be to 1.1% by weight, but is not limited thereto.

The glycolic acid is for improving keratin, antibacterial effect and acidity control, and the content of glycolic acid is 0.04 to 0.2% by weight, preferably 0.07 to 0.17% by weight, more preferably 0.1 to 0.14% by weight. However, it is not limited thereto.

Each component further included in the composition has excellent moisturizing and elasticity improvement effects within each content range, and further, skin soothing and acne improvement effects are excellent.

In addition, according to an embodiment of the present invention, the composition of the present invention comprises 0.1 to 0.4% by weight of a glucose and fructose mixture, 1 to 7% by weight of a mixture of Schisandra chinensis, ginger, yellow lotus and green tea extract, and Propolis and Portulaca Oleracea ) may further contain 0.1 to 2% by weight of the extraction mixture.

The glucose and fructose mixture is for reducing skin irritation and controlling sebum production, and the mixing ratio of glucose and fructose is not particularly limited, and the content of the glucose and fructose mixture is 0.1 to 0.4% by weight, preferably 0.2 to It may be 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

The extract mixture of Schisandra chinensis, ginger, Coptis chinensis, and green tea extract is for natural moisturizing, antibacterial and anti-inflammatory effects, and the content ratio of Schisandra chinensis, thought, Coptis chinensis and green tea extract is not particularly limited. The content of may be 1 to 7% by weight, preferably 3 to 5% by weight, more preferably 3.5 to 4.5% by weight, but is not limited thereto.

As the propolis and Portulaca Oleracea extraction mixture components for improving skin problems and irritation, increased moisture, the mixing ratio is not particularly limited, the propolis (Propolis) and Portulaca Oleracea (Portulaca Oleracea) content of the extraction mixture is from 0.1 to 2 % By weight, preferably 0.5 to 1.5% by weight, more preferably 0.9 to 1.1% by weight, but is not limited thereto.

Each component further included in the composition has excellent moisturizing and elasticity improvement effects within each content range, and further, skin soothing and acne improvement effects are excellent.

In addition, according to an embodiment of the present invention, the composition of the present invention is for improving acne.

The composition according to an embodiment of the present invention specifically has antibacterial activity against acne bacteria, for example, Propionibacterium acnes, inhibits lipid synthesis, reduces sebum, and improves inflammation.

In addition, the composition according to an embodiment of the present invention may further include a solvent, a thickener, an antifreeze agent, a moisturizing agent, a fragrance, a preservative, a surfactant, an antioxidant, a blood circulation promoting agent, and an additive.

The solvent may be water, preferably distilled water, and the content of the solvent may be 65 to 95% by weight, preferably 70 to 90% by weight, more preferably 75 to 85% by weight, but is limited thereto. no.

The thickener is at least one of sodium polyacrylate or carbomer, and the content of sodium polyacrylate is 0.05 to 0.35% by weight, preferably 0.1 to 0.3% by weight, more preferably May be 0.15 to 0.25% by weight, and the content of the carbomer may be 0.1 to 0.7% by weight, preferably 0.2 to 0.6% by weight, more preferably 0.3 to 0.5% by weight, but is limited thereto. It is not. The sodium polyacrylate and carbomer may also serve as a stabilizer for the composition according to an embodiment of the present invention.

The cryoprotectant may be glycerin, and the content of glycerin may be 1 to 5% by weight, preferably 2 to 4% by weight, more preferably 2.5 to 3.5% by weight, but is not limited thereto. . The glycerin may also serve as a desiccant for the composition according to an embodiment of the present invention.

The moisturizing agent may be at least one selected from the group consisting of betaine, 1.3 Butylene Glycol, and hyaluronic acid, and the content of betaine is 0.1 to 1.1% by weight, preferably 0.4 to 0.8% by weight, more preferably May be 0.5 to 0.7% by weight, and the content of 1.3 Butylene Glycol may be 0.5 to 2.5% by weight, preferably 1 to 2% by weight, more preferably 1.3 to 1.7% by weight, and the content of hyaluronic acid Silver may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight, but is not limited thereto.

The preservatives include Triclosan, Butylene Glycol, Capryl Hydroxamic Acid, 1,2-hexanediol, and Indian frankincense extract (Boswellia Serrata). Resin Extract), and the content of triclosan may be 0.1 to 3% by weight, preferably 0.5 to 2.5% by weight, more preferably 1 to 2% by weight, and the butyl Selected from the group consisting of Butylene Glycol, Capryl Hydroxamic Acid, 1,2-Hexanediol, and Boswellia Serrata Resin Extract. The content of at least one may be 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.2% by weight, but is not limited thereto.

The surfactant may be polyoxyethylene (60) hydrogenated castor oil, and the content of the polyoxyethylene (60) hydrogenated castor oil is 0.1 to 0.4% by weight, preferably 0.2 To 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

The antioxidant may be vitamin E/acetate, and the content of vitamin E/acetate may be 0.01 to 0.15% by weight, preferably 0.04 to 0.12% by weight, more preferably 0.06 to 0.1% by weight, but is limited thereto. It does not become.

The blood circulation promoter may be a hawthorn extract, and the content of the hawthorn extract may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight, but is limited thereto. no. The hawthorn extract may relieve skin itching.

The additive may be at least one of cetearyl alcohol or L-arginine, and the content of cetearyl alcohol is 0.01 to 0.2% by weight, preferably 0.05 to 0.15% by weight, More preferably, it may be 0.08 to 0.12% by weight, and the content of L-arginine may be 0.26 to 0.7% by weight, preferably 0.38 to 0.58% by weight, more preferably 0.46 to 0.5% by weight, but is limited thereto. It does not become. The cetearyl alcohol may increase the flexibility of the composition according to an exemplary embodiment of the present invention, and the L-arginine may be used to control the acidity of the composition according to an exemplary embodiment of the present invention.

In addition, the composition of the present invention may additionally contain components conventionally added to the cosmetic composition, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and conventional adjuvants and carriers such as fragrances.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils , Powder foundation, emulsion foundation, wax foundation, and spray may be formulated, but are not limited thereto. More specifically, a lotion such as a flexible lotion or a nutrient lotion, an emulsion such as a facial lotion, a body lotion, a cream such as a nourishing cream, a moisture cream, an eye cream, an essence, a cosmetic ointment, a spray, a gel, a pack, a sunscreen, and a make-up. It can be prepared in a formulation such as a foundation, powder, etc., such as a base, a liquid type, a solid type, or a spray type. In this case, the cosmetic composition is an emulsifier, a soap acid, a solvent, a colorant, a preservative, an antioxidant, an antifoaming agent, an antibacterial agent, an anti-redeposition agent, an enzyme, a plant or mineral oil, a fat, a fluorescent substance, a fungicide, a hydrophobic substance, a moisturizer, It may contain excipients including fragrances, fragrance carriers, preservatives, proteins, silicones, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, waxes, and the like.

When the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide, etc. are used as carrier components. Can be used.

When the formulation of the cosmetic composition is a powder or spray, toss, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, Propellants such as propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

Hereinafter, examples will be described in detail in order to describe the present invention in detail.

Preparation Example-Preparation of the cosmetic composition of the present invention

A first mixture was obtained by dispersion injection of 0.6 kg of Cosmedia SP (Daebong LS, Korea) as sodium polyacrylate and 1.2 kg of Carbopol 940 as Carbomer in 233.52 kg of distilled water as a solvent in the dissolution tank.

Later, as Dipotassium Glycyrrhizate, DPG-2K (BioR&G, Korea) 0.12kg, vitamin B6/HCL (Lee Hyun Chemical, Korea) 0.18kg, glycerin 9kg, Betaine (Bioal) Angie, Korea) 1.8 kg, niacin amide (Lee Hyun Chemical, Korea) 1.5 kg, and methyl sulfonyl methane (MSM) (BioR&G, Korea) 0.9 kg in the dissolution tank in that order, and the second mixture was added. Got it.

Water Lily 0.18kg, Triclosan (Lee Hyun Chemical, Korea) 0.45kg, Cetearyl alcohol as Kalcol 6870 0.3kg, Salicylic Acid (BioR&G, Korea) 0.36kg, Polyoxyethylene (60) Hydrogenated HCO-60 0.75kg, vitamin E/acetate (Lee Hyun Chemical, Korea) 0.24kg, camellia oil (BioR&G, Korea) 0.6kg and 1.3 Butylene Glycol 4.5kg as 60 The mixture was heated and melted in a separate melting tank at °C to obtain a third mixture. Thereafter, after cooling the third mixture to 40°C, it was poured into the dissolution tank and sufficiently stirred to obtain a fourth mixture.

1% Hyal Oligo (Shin Abity, Korea) 1.5 kg as hyaluronic acid, Pentavitin (Interless, Korea) 0.6 kg as a mixture of glucose and fructose (Saccharide Isomerate), butylene glycol (Butylene Glycol), capryl hydrooxamic acid (Capryl Hydroxamic Acid), 1,2-Hexanediol and Boswellia Serrata Resin Extract, Bioneat WS (BioR&G, Korea) 3kg, Epilobium Playcery Extract ( Epilobium Fleischeri Extract) ALP-SEBUM (Interless, Korea) 0.45kg, Aloe Leaf Extract (Bioland, Korea) 3kg, Hawthorn extract as Na3 Hawthorn (Dami Chemical, Korea) 1.5kg, Location Witch Hazel Extract (Bioland, Korea) 3kg, Bio Protector (Dr. Lanka, Korea) 12kg, as a mixture of Schisandra chinensis, ginger, yellow lotus and green tea extract, Willow Bark Extract (BioR&G, Korea) ) 1.2kg, Propolis and Portulaca Oleracea extract mixture, Oriental Trois 2PK (BioR&G, Korea) 3kg, Glycolic Acid (BioR&G, Korea) 0.36kg and chamomile extract (Camomile) Extract) (Shin Abity, Korea) 0.75 kg was sequentially injected into the dissolution tank and stirred to obtain a fifth mixture.

In another separate dissolution tank, 1.44 kg of L-arginine (Hyun Chemical Lee, Korea) was dissolved in 12 kg of distilled water as a solvent to obtain a sixth mixture.

Thereafter, the sixth mixture was injected into the dissolution tank, degassed and mixed for 30 minutes to obtain 300 kg of the composition of the present invention.

Examples and Comparative Examples

According to the preparation example, Examples 1 to 3 and Comparative Examples 1 to 6 were prepared with the components and contents shown in Table 1 below.

At this time, each extract was obtained by adding 70% ethanol 20 times the content of each material, hot water extraction at 100°C in a heating extractor for 3 hours, and freeze-drying the extract.

Dipotassium glycyrrhizate
(Dipotassium Glycyrrhizate) Camellia oil
(Camellia Oil) Epilobium Playcery Extract
( Epilobium Fleischeri Extract) Witch hazel extract
(Witch Hazel Extract) Willow bark extract
(Willow Bark Extract) Chamomile extract
(Camomile Extract) Centella asiatica Urbain extract Example 1 0.04% by weight 0.2% by weight 0.15% by weight 1% by weight 0.4% by weight 0.25% by weight - Example 2 0.05% by weight 0.3% by weight 0.2% by weight 1.5% by weight 0.6% by weight 0.3% by weight - Example 3 0.03% by weight 0.1% by weight 0.1% by weight 0.5% by weight 0.2% by weight 0.2% by weight - Comparative Example 1 - 0.2% by weight 0.15% by weight 1% by weight 0.4% by weight 0.25% by weight 0.04% by weight Comparative Example 2 0.04% by weight - 0.15% by weight 1% by weight 0.4% by weight 0.25% by weight 0.2% by weight Comparative Example 3 0.04% by weight 0.2% by weight - 1% by weight 0.4% by weight 0.25% by weight 0.15% by weight Comparative Example 4 0.04% by weight 0.2% by weight 0.15% by weight - 0.4% by weight 0.25% by weight 1% by weight Comparative Example 5 0.04% by weight 0.2% by weight 0.15% by weight 1% by weight - 0.25% by weight 0.4% by weight Comparative Example 6 0.04% by weight 0.2% by weight 0.15% by weight 1% by weight 0.4% by weight - 0.25% by weight Sodium Polyacrylate 0.2% by weight
Carbomer 0.4% by weight
Glycerin 3% by weight
Betaine 0.6% by weight
1.3 Butylene Glycol 1.5% by weight
0.5% by weight of hyaluronic acid
Triclosan 1.5% by weight
Butylene Glycol, Capryl Hydroxamic Acid, 1,2-Hexanediol and Boswellia Serrata Resin Extract 1% by weight
Polyoxyethylene (60) Hydrgenated Castor Oil 0.25% by weight
Vitamin E/acetate 0.08% by weight
Hawthorn extract 0.5% by weight
Cetearyl alcohol 0.1% by weight
L-Arginine 0.48% by weight
Distilled water remaining amount

In addition, Example 4 contains the following components and contents instead of 1.2% by weight of distilled water of Example 1, and was prepared according to Preparation Example 1: Vitamin B6/HCl 0.06% by weight, niacin amide 0.5% by weight, methyl Sulfonyl Methane (Methyl Sulfonyl Methane, MSM) 0.3% by weight, salicylic acid (Salicylic Acid) 0.12% by weight, Aloe Leaf Extract (Aloe Leaf Extract) 0.1% by weight and glycolic acid (Glycolic Acid) 0.12% by weight.

Comparative Example 7 includes 1.2% by weight of centella asiatica Urbain extract instead of 1.2% by weight of distilled water of Example 1, and was prepared according to Preparation Example 1.

In addition, Example 5 includes the following components and contents instead of 5.25% by weight of distilled water of Example 4, and was prepared according to Preparation Example 1: 0.25% by weight of a glucose and fructose mixture, Schisandra chinensis, ginger, yellow lotus and green tea extract mixture 4% % And 1% by weight of Propolis and Portulaca Oleracea extract mixture.

Comparative Example 8 contained 5.25% by weight of centella asiatica Urbain extract instead of 5.25% by weight of distilled water of Example 4, and was prepared according to Preparation Example 1.

Experimental example

1. Antibacterial activity test against acne bacteria

Antimicrobial activity against propionibacterium acnes (ATCC 6919: medium-BHI broth), which is a strain causing acne, with the cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 8, and erythrombomycin, a known acne treatment, as a control Was tested. The antimicrobial test method for acne bacteria was as follows.

(1) Preparation of test bacteria solution

Propionibacterium acnes was inoculated into BHI broth and cultured anaerobicly.

(2) Preparation of diluted solution

BHI broth (pH 6.8) or LB broth (pH 4.5) was mixed well by adding 0.15 ml of the test bacteria solution to 15 ml of the LB broth (pH 4.5), and the diluted solution was used.

(3) sample preparation

The cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 8, and the erythrombomycin undiluted solution were used as samples.

(4) Antibacterial activity test

1) Put the sample in line 1 of a 96-well cell culture tube (96 well plate) to match the starting concentration, and add 200 μl each as a diluted solution.

2) After mixing the mixture solution from row 1 well, take 100 μl, put it in row 2, mix well, and then take 100 μl again and put it in row 3 to double dilution.

3) After stationary culture at 32°C for 24 hours and 48 hours, determine the presence or absence of the growth of the bacteria to the degree of suspension, and determine the minimum concentration without the growth of the bacteria as the MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine the presence or absence of bacterial growth, check through microscopic observation.

The results of the antibacterial activity test against acne bacteria are shown in Table 2 below. MIC was expressed in terms of the concentration of the active ingredient contained in the formulation.

division Prisonibacterium acnes (MIC) Example 1 87 ppm Example 2 85 ppm Example 3 89 ppm Example 4 75 ppm Example 5 61 ppm Comparative Example 1 132 ppm Comparative Example 2 141 ppm Comparative Example 3 130 ppm Comparative Example 4 124 ppm Comparative Example 5 138 ppm Comparative Example 6 126 ppm Comparative Example 7 81 ppm Comparative Example 8 72 ppm Erythrombomycin 100 ppm

The smaller the ppm concentration in MIC is, the more effective it is to the antibacterial activity against acne bacteria.In Examples 1 to 5, the ppm concentration was lower than that of erythrombomycin and Comparative Examples 1 to 6, which are known acne treatments. Example 4 was smaller than Comparative Examples 1 to 7 and Example 5 was smaller than Comparative Examples 1 to 8, and it was confirmed that the composition of the present invention has excellent antibacterial activity against the test bacteria.

2. Lipogenesis inhibition test

6 containing DMEM (Dulbecos modified eagles medium, GIBCO BRL, Life Technologes) medium containing 10% fetal bovine serum (FBS) for 3T3-L1 cells, a fibroblast cell line from mice. It was attached to a well culture plate at 1×10 ^{5 cells/well.} After 2 days, it was replaced with a new DMEM (containing 10% FBS) medium and cultured for 2 days. Then, the cultured cells were again induced to differentiate with DMEM (containing 10% FBS) containing 1 μg/ml insulin, 0.5 mM IBMX, and 0.25 μM dexamethasone, and after 2 days had elapsed again. It was exchanged for DMEM containing insulin and incubated for 5 days. After 5 days, it was exchanged with a normal medium (containing DMEM, 10% FBS) and cultured while observing until the cells changed into adipocytes morphologically.

Sudan III staining (S4136, sigma-aldrich) was performed using 3T3-L1 adipocytes differentiated above to evaluate the efficacy of the cosmetic composition according to the present invention to inhibit fat accumulation in adipocytes. Adipocytes were fixed in phosphate buffer with 4% paraformaldehyde (pH 7.2) at room temperature, washed with PBS (phsphate buffered saline), stained with Sudan III, and photographed for visual comparison. As a control group, only the medium to which the compositions of Examples or Comparative Examples were not added was used, and 50 μM of caffeine was treated as another control group. The degree of inhibition of fat accumulation was graded by dividing the degree of staining by +++, ++, +, -. The results are shown in Table 3.

sample Inhibition rate (%) Control +++ Comparative group + Example 1 + Example 2 + Example 3 + Example 4 + Example 5 + Comparative Example 1 ++ Comparative Example 2 ++ Comparative Example 3 ++ Comparative Example 4 ++ Comparative Example 5 ++ Comparative Example 6 ++ Comparative Example 7 + Comparative Example 8 +

As shown in Table 3, Examples 1 to 5 of the present invention were found to have a lipid synthesis inhibitory effect, and were stronger than Comparative Examples 1 to 6. However, Comparative Examples 7 and 8 showed similar levels to Examples 1 to 5. Therefore, the composition according to an embodiment of the present invention can suppress the occurrence of acne by reducing sebum by inhibiting lipid synthesis.

3. Acne improvement, sebum secretion reduction, and irritation test

Through medical history survey and skin condition diagnosis, a panel consisting of 50 adult men and women in their 20s to 40s suitable for the test purpose was allowed to use the cosmetic compositions and erythrombomycin of Examples 1 to 5 and Comparative Examples 1 to 8 for one month. . The acne improvement scale was set from 1 to 5 points, with 1 point as ‘No,’, 3 points as ‘normal’, and 5 points as ‘very yes.’ The experimental results are expressed as the average score of 50 people in Table 4 below.

Acne disappearance period was based on the number of days that disappearance was read, and acne recurrence was based on the result after 1 month with or without acne. The reduction in sebum secretion was set from 1 to 5 points, and 1 point was marked as ‘No,’ for 3 points, ‘It is normal,’ and 5 points for ‘Very so.’ The experimental results are expressed as the average score of 50 people in Table 4 below. The presence or absence of skin irritation was expressed as (number of persons who showed irritation reaction)/(total number of test subjects).

Improvement of inflammatory acne The time of disappearance of comedonic acne Acne recurrence Reduction of sebum secretion Presence or absence of stimulation Example 1 4.1 3 days radish 4.2 0/50 Example 2 4.3 3 days radish 4.4 0/50 Example 3 4.0 3 days radish 4.0 0/50 Example 4 4.4 2 days radish 4.6 0/50 Example 5 4.7 2 days radish 4.7 0/50 Comparative Example 1 3.1 8 days radish 3.0 2/50 Comparative Example 2 3.2 8 days U 3.2 5/50 Comparative Example 3 2.8 9 days radish 2.6 4/50 Comparative Example 4 3.0 11th U 2.8 8/50 Comparative Example 5 2.9 10 days radish 3.3 5/50 Comparative Example 6 3.3 9 days U 3.4 7/50 Comparative Example 7 4.3 3 days radish 4.4 0/50 Comparative Example 8 4.5 2 days radish 4.6 0/50 Erythrombomycin 4.2 2 days radish 4.1 36/50

As shown in Table 4, in Examples 1 to 5, unlike Comparative Examples 2, 4, and 6, acne did not recur, Examples 1 to 5 were compared to Comparative Examples 1 to 6, and Example 4 was compared to Comparative Examples 1 to 6 Rather than 7, it was found that Example 5 has an excellent effect on improving acne overall than Comparative Examples 1 to 8. On the other hand, in the case of erythrombomycin, which is an antimicrobial standard substance, exhibits similar effects to those of Examples 1 to 5, however, it is highly irritating in use, so there is a concern of skin irritation for long-term use.

4. Inflammation improvement effect

The anti-inflammatory effect was evaluated as the inhibitory effect of prostaglandin production. Using Examples 1 to 5 and Comparative Examples 1 to 8, the effect of macrophages was measured. First, aspirin was added to the macrophages collected from the peritoneal cavity of the mouse so that the final concentration was 500M to irreversibly inhibit the activity of cyclooxygenase (COX) remaining in the cells. Then, 100 μl of the suspension was added to each well of a 96-well cell culture tube _{and incubated for 2 hours in an incubator under conditions of 5% CO 2} and 37° C. to attach macrophages to the surface of the container. Subsequently, the attached macrophages were washed three times with PBS and then used for the effect test of the extract. ^{After incubating for 12 hours by adding RPMI medium containing 1% (w/v) LPS to 5x10 4} cells/ml of the cultured macrophages, the production of prostaglandin was induced, and the extract was treated with 100 μl to enzymatic free prostaglandin. Quantification was performed using an immunoassay (ELISA).

At this time, the prostaglandin production inhibitory activity of the extract is set to 100%, and the difference between the prostaglandins produced in the LPS-treated and untreated groups is set to 100%, and the percentage of prostaglandins reduced by treatment with LPS and the sample is used as the control group. It was compared with and determined, and the results (effect of inhibiting the production of prostaglandin) are shown in Table 5 below.

Blank 100% Control group (aspirin treatment group) 25.0% Example 1 31.1% Example 2 29.2% Example 3 32.5% Example 4 26.6% Example 5 24.5% Comparative Example 1 43.2% Comparative Example 2 41.2% Comparative Example 3 44.4% Comparative Example 4 38.3% Comparative Example 5 40.1% Comparative Example 6 39.8% Comparative Example 7 28.7% Comparative Example 8 26.2%

As shown in Table 5, as a result of the experiment, when Examples 1 to 3 were treated as in the control group treated with aspirin, the inhibitory effect of prostaglandin production was compared to Comparative Examples 1 to 6, and when Example 4 was treated Comparative Example 1 Compared to 7 to 7, it was found that the treatment of Example 5 was higher than that of Comparative Examples 1 to 8. Through this, it was found that the cosmetic composition according to an embodiment of the present invention can provide an excellent inflammation improvement effect.

5. Skin soothing, moisturizing and elasticity improvement effect-Panel evaluation

The composition (serum) of Examples 1 to 5 and Comparative Examples 1 to 8 was used for a panel consisting of 50 adult men and women in their 20s to 40s suitable for the purpose of the test through medical history survey and skin condition diagnosis, reducing waste products, Soothing, moisturizing, and elasticity were evaluated.

Take an appropriate amount of the serum of Examples 1 to 3 and Comparative Examples 1 to 6, apply it to the nose and surrounding skin, leave for 10 to 15 minutes, and apply once a day for 4 weeks by lightly washing the face with warm water. According to the panel's opinion, the degree of improvement was evaluated in a five-stage discrimination method (1: very bad, 2: bad, 3: moderate, 4: good, 5: very good), and the average values are shown in Table 6 below.

division Moisturizing elasticity Calm Example 1 4.2 4.1 4.1 Example 2 4.3 4.3 4.2 Example 3 4.0 4.1 4.0 Example 4 4.5 4.6 4.4 Example 5 4.7 4.8 4.5 Comparative Example 1 3.2 3.3 2.9 Comparative Example 2 3.0 3.2 3.1 Comparative Example 3 3.1 3.0 3.1 Comparative Example 4 2.8 3.1 2.9 Comparative Example 5 3.2 2.9 3.1 Comparative Example 6 3.5 3.2 3.3 Comparative Example 7 4.4 4.3 4.1 Comparative Example 8 4.7 4.7 4.8

Referring to Table 6, the cosmetic compositions of Examples 1 to 3 are more than the cosmetic compositions of Comparative Examples 1 to 6, the cosmetic composition of Example 4 is more than the cosmetic compositions of Comparative Examples 1 to 7, and the cosmetic composition of Example 5 is It was confirmed that overall moisturizing, elasticity, and soothing effects were superior to the cosmetic compositions of Comparative Examples 1 to 8.

Claims (4)

Dipotassium Glycyrrhizate 0.01 to 0.07 wt%, Camellia Oil 0.01 to 0.4 wt%, Epilobium Fleischeri Extract 0.05 to 0.25 wt%, Witch Hazel extract (Witch Hazel) Extract) 0.1 to 2% by weight, willow bark extract (Willow Bark Extract) 0.1 to 0.7% by weight and chamomile extract (Camomile Extract) containing 0.1 to 0.4% by weight, cosmetic composition.
The method according to claim 1, Vitamin B6 / HCl 0.02 to 0.1% by weight, niacin amide (Niacin Amide) 0.1 to 1% by weight, methyl sulfonyl methane (Methyl Sulfonyl Methane, MSM) 0.1 to 0.5% by weight, salicylic acid (Salicylic acid) 0.04 To 0.2% by weight, aloe leaf extract (Aloe Leaf Extract) 0.1 to 2% by weight and glycolic acid (Glycolic Acid) 0.04 to 0.2% by weight further comprising, a cosmetic composition.
The method of claim 2, further comprising 0.1 to 0.4% by weight of a glucose and fructose mixture, 1 to 7% by weight of a mixture of Schisandra chinensis, ginger, Coptis chinensis and green tea extract, and 0.1 to 2% by weight of a mixture of Propolis and Portulaca Oleracea extract. Containing, cosmetic composition.
The method according to claim 1, wherein the composition is for acne improvement, cosmetic composition.