KR20210012529A - Anti-bacterial composition for Streptococcus mutans and composition for removing halitosis using the same - Google Patents

Abstract

The present invention discloses: an antibacterial composition against Streptococcus mutans, a causative agent of dental caries, using a Platycladus orientalis leaf extract, an Eriobotrya japonica leaf extract, etc.; and a composition for removing halitosis using the same.

Description

Anti-bacterial composition for Streptococcus mutans and composition for removing halitosis using the same}

The present invention relates to an antibacterial composition against Streptococcus mutans bacteria and a composition for removing bad breath using the same.

The importance of oral health care to improve the quality of life of the elderly is increasing as the average life span is extended due to the improvement of the people's living standard and the development of medical technology. Dental caries and periodontal diseases are representative diseases that can cause dental loss due to oral health. According to data from the Ministry of Health and Welfare in 2013, the rate of permanent dental caries experience among adult men in Korea was 87%, and 90% for women. In addition, the prevalence of periodontal disease in Korean adults over the age of 30 decreased from 2007 to 2012 and then increased again in 2013, and it was found that one out of three adults was relaxed from periodontal disease.

Dental caries is experienced by more than 80% of the world's population, and its incidence has been continuously decreasing since the 1970s in some developed countries in the West, but it is still increasing in Korea, Eastern Europe, and some African regions (Nishi et al., 2002 Community Dent. Oral Epidemiol 2002 30:296-301.). Dental caries not only causes various pains, but also causes apical end, alveolar bone destruction and tooth loss if it continues to progress. The occurrence of dental caries is deeply related to dietary habits and microbes in the oral cavity. Among about 750 species of microorganisms living in the human oral cavity, the causative agent representing dental caries is known as Streptococcus mutans (Loesche WJ. Medical) Microbiology 1996 (4th ed.);Kilian Clarke J. Br J Exp Pathol. 1924 Jun; 5(3): 141147.; Hyeyoung Kim, Ph.D. Thesis, Graduate School, Seoul National University 2002).

When Streptococcus mutans encounters sugar in the oral cavity, polysaccharide crystals are formed between teeth and stored. It attaches to the tooth capsule and synthesizes insoluble glucan by the action of glucosyltransferase (GTase) enzyme. Synthesized glucan increases the binding power between bacteria, and lactic acid formed as metabolic waste accumulates under polysaccharide crystals and is concentrated. This concentrated high concentration of lactic acid is known to cause dental caries by dissolving Ca ²⁺ in calcium phosphate, which is a component of the tooth, and punctures the enamel on the tooth surface (Sigmund, SS et al., 1992 J. Periodontol. 63 :322-331.; Kim et al., Hyunmoonsa 2009 19:307-316). Accordingly, in order to prevent dental caries, search for an effective antibacterial agent for Streptococcus mutans or a substance possessing the inhibitory activity of GTase is being actively conducted.

When taking general antibacterial agents continuously to treat dental caries, a chronic disease, various side effects such as digestive problems, hypersensitivity reactions, kidney toxicity, oral toxicity, and tooth discoloration may occur. The search for materials is constantly being tried. Accordingly, Korean Registered Patent No. 1015449390000 discloses a composition for preventing or treating dental caries containing Jinkyo extract as an active ingredient, and Korean Patent No. 1015069950000 discloses an orinal bark extract, and Korean Patent No. 1013158050000 discloses a composition for preventing or treating dental caries. Korean Patent Registration No. 1012952420000 discloses a composition for preventing or treating dental caries, comprising an oral extract as an active ingredient. .

The present invention discloses antibacterial activity, such as a cypress leaf extract, against Streptococcus mutans, which is a causative agent of carious caries.

It is an object of the present invention to provide an antibacterial composition against Streptococcus mutans using a cypress leaf extract.

Another object of the present invention is to provide a composition for removing bad breath using a cypress leaf extract.

Other or specific objects of the present invention will be presented below.

The present invention, as confirmed in the following Examples and Experimental Examples, by the agar plate diffusion method (disc-agar plate diffusion method), the cypress leaf extract, the loquat leaf extract, and a mixture thereof, strepto, which is the causative agent of dental caries. It has antibacterial activity against S. mutans and other harmful microorganisms such as E. coli , Salmonella enterica , Vibrio parahaemolyticus , Bacillus cereus It has an antibacterial activity against ( B. cereus ), and has an antibacterial effect for oral cleanliness, an effect of removing bad breath, etc., as well as low irritation and excellent taste such as flavor.

In consideration of the above, the present invention, in one aspect, as an antibacterial composition or a composition for improving dental caries against Streptococcus mutans comprising a cypress leaf extract, a loquat leaf extract, or a mixture thereof as an active ingredient. In another aspect, it can be understood as a halitosis remover composition comprising a cypress leaf extract, a loquat leaf extract, or a mixture thereof as an active ingredient, and in another aspect, a cypress leaf extract, a loquat leaf extract, or It can be understood as an antibacterial composition for Streptococcus mutans, Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus, and Bacillus cereus, including a mixture of these as an active ingredient.

In the present specification, the term "extract" refers to water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, Extract obtained by leaching using N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, using a supercritical extraction solvent such as carbon dioxide, pentane, etc. It means an extract obtained, a fraction obtained by fractionating the extract, or an essential oil extract, and the extraction method uses an arbitrary method such as cold sedimentation, reflux, warming, ultrasonic radiation, supercritical extraction, etc. in consideration of the polarity of the active material, the degree of extraction, and Can be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and then mixing and policing it with a solvent having a different polarity, and adsorbing the crude extract on a column filled with silica gel, etc., and then adding a hydrophobic solvent, a hydrophilic solvent, or a mixed solvent thereof. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or a solid extract from which the extraction solvent has been removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it means an extract obtained by extraction with water, ethanol, or a mixed solvent thereof, particularly an aqueous solution of 60% to 90% ethanol, or an essential oil extract as an extraction solvent. In general, essential oil extract refers to an oily extract obtained by condensing volatile components. For the preparation of the essential oil extract, methods known in the art, such as steam distillation method, solvent extraction method using volatile solvents (benzene, hexane, etc.), and supercritical extraction method, may be used.

In addition, in the present specification, the term "active ingredient" refers to an ingredient capable of exhibiting a desired activity alone or with a carrier that is not itself active.

In addition, in the present specification, the term "improving dental caries" means preventing, treating or reducing symptoms of dental caries, which is a target disease.

In addition, in the present specification, "antibacterial" is meant to include induction or inhibition of proliferation of the corresponding microorganism.

The antimicrobial composition of the present invention or the composition for improving periodontal caries (hereinafter collectively referred to as "composition of the present invention") can exhibit the intended antimicrobial activity, dental caries improvement activity, etc. according to the use, formulation, and purpose of mixing the active ingredient. It can be included in one arbitrary amount (effective amount), and a typical effective amount will be determined within the range of 0.001% to 15% by weight based on the total weight of the composition. Here, "effective amount" refers to the intended medical and pharmacological effects such as improvement of dental caries, etc., when the composition of the present invention is administered to a mammal, preferably a human, to which the composition of the present invention is administered during the administration period according to the advice of a medical expert, etc. It refers to the amount of active ingredient that can be expressed. Such effective amounts can be determined empirically within the range of ordinary skill in the art.

The composition of the present invention can be grasped as a food composition in a specific aspect.

The food composition of the present invention can be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, ionized beverages, processed oils such as milk, yogurt, gums, rice cakes, Korean sweets, bread, sweets, noodles, etc. Foods, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jelly, and health functional food preparations such as bars can be prepared. In addition, the food composition of the present invention can be classified as a product as long as it conforms to the enforcement regulations at the time of manufacture and distribution in terms of legal and functional classification. For example, it is a health functional food in accordance with the Health Functional Food Act, or confectionery, beans, soy milk, fermented beverages, special purpose foods, etc. according to each food type according to the Food Sanitation Act (Notice of the Ministry of Food and Drug Safety, food standards and standards). I can.

The food composition of the present invention may contain food additives in addition to the active ingredients. Food additives are generally understood as substances that are mixed or infiltrated by being added to food in manufacturing, processing, or preserving food. Since it is taken daily and for a long time with food, its safety must be ensured. In the Food Additives Code according to the Food Sanitation Act (notified by the Ministry of Food and Drug Safety, standards and standards for food additives), food additives with guaranteed safety are classified into chemical synthetic products, natural additives, and mixed preparations.

These food additives can be classified into sweeteners, flavoring agents, preservatives, emulsifiers, acidulants, and thickeners in terms of their functionality.

Sweeteners are used to impart a suitable sweetness to food, and natural or synthetic ones may be used. Preferably, a natural sweetener is used, and examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents can be used to improve taste or flavor, and both natural and synthetic can be used. Preferably, it is the case of using a natural one. In the case of using natural ingredients, the purpose of nutrient enhancement can be combined in addition to flavor. As a natural flavoring agent, it may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or may be obtained from green tea leaves, roundtails, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, you can use those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo. The natural flavoring agent may be a liquid concentrate or a solid extract. In some cases, synthetic flavoring agents may be used, and esters, alcohols, aldehydes, terpenes, and the like may be used as synthetic flavoring agents.

As a preservative, sodium calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, Pectin, etc. may be mentioned, and as the acidulant, arithmetic, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like can be used. The acidulant may be added so that the food composition has an appropriate acidity for the purpose of suppressing the growth of microorganisms in addition to the purpose of enhancing taste.

As the thickening agent, a suspending agent, a settling agent, a gel-forming agent, a swelling agent, and the like may be used.

In addition to the food additives described above, the food composition of the present invention may include a physiologically active substance or minerals known in the art for the purpose of supplementing and reinforcing functionality and nutritional properties and ensuring stability as a food additive.

Examples of such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoyl thiamine, etc., and minerals include calcium preparations such as calcium citrate, magnesium stearate. Magnesium preparations such as, iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, and the like.

In the food composition of the present invention, the food additive as described above may be included in an appropriate amount to achieve the purpose of addition according to the product type.

With respect to other food additives that may be included in the food composition of the present invention, reference may be made to the food code or the food additive code.

The composition of the present invention may be understood as a pharmaceutical composition in other specific embodiments.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier in addition to the active ingredient, including quasi-drug formulations such as oral cleansing composition formulations or toothpaste composition formulations, oral formulations, parenteral formulations, etc. by a conventional method known in the art. It can be prepared as a pharmaceutical formulation. Here, the meaning of "pharmaceutically acceptable" means that the application (prescription) does not have toxicity beyond adaptable without inhibiting the activity of the active ingredient.

When the composition of the present invention is prepared in a mouthwash composition formulation or toothpaste composition formulation, it may be prepared in a paste form, powder form, spray form or liquid (gargle form), and in addition to containing carnosic acid, which is an active ingredient of the present invention, It may further include any one or more components selected from the group consisting of a tooth decay prevention drug component, an abrasive component, a humectant component, a binder component, a foam component, a sweetener component, a flavor component, and a coloring component.

Any one or more components selected from the group consisting of sodium fluoride, sodium fluoride phosphate, amine fluoride, tin fluoride, chlorohexidine, cetylpyridinium chloride, triclosan, xylitol, bamboo salt, and propolis can be used as the caries-preventing drug ingredient. have.

In addition, as the abrasive component, selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate, and aluminum silicate. Any one or more components to be used may be used.

In addition, any one or more selected from the group consisting of glycerin, sorbitol, xylitol, polyethylene glycol, and propylene glycol may be used as the humectant component. This humectant ingredient is an essential base ingredient for making a paste formulation. It prevents the toothpaste from drying and solidifying when exposed to the air, provides shine to the surface of the toothpaste, and, depending on the type, plays a role of giving a sweetening effect when brushing teeth. .

In addition, any type of water-soluble polymer may be used as the binder component. Preferably, sodium carboxymethylcellulose, carrageenan, xanthan gum, and the like may be used. The binder serves to prevent separation of the solid powder component and the liquid component.

In addition, as the foaming agent component, it is preferable to use sodium lauryl sulfate, an anionic surfactant, and depending on the characteristics of the formulation, a copolymer of polyoxyethylene polyoxypropylene (poloxamer), polyoxyethylene hydrogenated castor oil or polyoxy Nonionic surfactants, such as ethylene sorbitan fatty acid ester, can be used. These foaming agents improve the feeling of use of the product, help cleansing, accelerate the dispersion and penetration of other medicinal ingredients, and reduce the interfacial tension, so that foreign substances in the oral cavity easily fall off.

In addition, the fragrance component, sweetener component, coloring component, etc. are for improving the feeling of use of the toothpaste composition, and it is preferable to use an edible component as the fragrance component, such as menthol, peppermint oil, spearmint oil, sage, eucalyptus Riptol, methyl salicylate, or fruit extract.

Moreover, it is preferable to use sodium saccharin as the sweetener component, and it is preferable to use food coloring as the pigment component.

When the pharmaceutical composition of the present invention is prepared in an oral dosage form, powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, suspensions, wafers according to a method known in the art together with a suitable carrier It can be prepared in such a formulation. Examples of suitable pharmaceutically acceptable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol and xylitol, starches such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, Celluloses such as sodium carboxymethylcellulose and hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable Yu, etc. are mentioned. In the case of formulation activity, if necessary, it may be formulated including diluents and/or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants.

When the pharmaceutical composition of the present invention is prepared in a parenteral formulation, it may be formulated in the form of an injection, a transdermal administration, a nasal inhalation and a suppository according to a method known in the art together with a suitable carrier. When formulated as an injection, suitable carriers include sterile water, ethanol, polyols such as glycerol or propylene glycol, or mixtures thereof, preferably Ringer's solution, PBS (phosphate buffered saline) containing triethanol amine, or sterile water for injection. , An isotonic solution such as 5% dextrose may be used. When formulated into a transdermal dosage form, it may be formulated in the form of an ointment, cream, lotion, gel, external solution, pasta, liniment, air roll, and the like. In the case of nasal inhalants, it can be formulated in the form of an aerosol spray using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, and carbon dioxide, and the bases of the suppositories are witepsol and tween. (tween) 61, polyethylene glycols, cacao butter, laurin paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters, and the like can be used.

The formulation of pharmaceutical compositions is known in the art, and for more specific information, reference may be made to Remington's Pharmaceutical Sciences (19th ed., 1995). These documents are considered as part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, sex, age, patient severity, and route of administration. May be in the /kg range. Administration can be made once a day or divided into several times. Such dosages are not to be construed as limiting the scope of the invention in any aspect.

As described above, according to the present invention, it is possible to provide an antibacterial composition against Streptococcus mutans bacteria using cypress leaves or the like. The composition of the present invention may be commercialized as quasi-drugs such as mouthwash and toothpaste, functional foods, and drugs.

1 to 5 are antibacterial activity results.
6 is a result of measuring the number of bacteria.
7 is an analysis result of oral bad breath gas.

Hereinafter, the present invention will be described with reference to examples. However, the scope of the present invention is not limited to these examples.

<Example> Antimicrobial activity test for oral microorganisms, etc., an experiment for measuring the number of bacteria in the oral cavity, an experiment for analyzing oral bad breath gas and an experiment for sensory evaluation

1. Sample and method

1.1 Sample

Ethanol extract samples, Thuja orientalis (extraction site: leaves), loquat tree ( Eriobotrya Japonica , extraction site: leaves) 10 times the weight of 80% ethanol was added to each dry powder, extracted at 80°C for 4 hours, and then filtered. After filtering with (Whatman #2), it was concentrated under reduced pressure and lyophilized to prepare a powder.

Hot-water extract samples were extracted for 4 hours at 100° C. in an extractor equipped with a reflux condenser into water and 1:10 times the weight of each dry powder of cypress leaves and loquat leaves. The extract was subjected to the same method as the ethanol extract sample to obtain a powder.

Essential oil extract samples were prepared by distilling extraction from hot water at 100° C. for 4 hours, respectively, for dry powders of cypress leaves and loquat leaves. The extraction conditions were SDE (simultaneous distillation and extraction) equipment. Sodium sulfate was added and filtered to remove moisture contained in the collected essential oil.

A mixture sample was obtained by mixing the ethanol extract and the essential oil extract at the same weight.

Prototype samples were prepared in weight percent as described in the table below. 5% ethanol was used as a preservative and solubilizing agent, and in consideration of use as a mouthwash, 80% ethanol extract of mung bean (prepared as above) having surfactant properties was added and used. To achieve 100% of the total amount of the prototype, sterilized water containing 0.0001% of the surfactant Tween 20 and 0.05% of NaF used to prevent tooth decay was applied equally to all treatment units to prepare a prototype.

Prototype composition (%, w/v) division Cypress oil Loquat
essential oil Side white
Ethanol extract Loquat
Ethanol extract green gram
extract 5% ethanol BP1 0.05 0.05 2.0 2.0 One 4 BP2 0.03 0.03 1.0 1.0 One 4 BP3 - - 1.0 1.0 One 4 BP4 - - 0.1 0.1 One 4

1.2 Antibacterial activity test

Antimicrobial activity is oral microbes streptococcus mutans and other harmful microbes E. coli , Salmonella enterica , Vibrio parahaemolyticus , and Bacillus. Cereus ( B. cereus ) was targeted.

As the medium, BHI (Brain Heart Infusion medium (Bacto BHI, BD, USA) was used for Streptococcus mutans, and LB medium (Bacto-LB, BD, USA) was used for other harmful microorganisms.

The antimicrobial activity test of the sample was performed using the disc-agar plate diffusion method. Specifically, the sample is dissolved in diethyl ether at a certain concentration, filtered through a 0.45 µm membrane filter (Milipore, USA), and absorbed at various concentrations at various concentrations on a sterilized filter paper disc (Toyo seisakusho, 8 ㎜), and then nitrogen gas is used. The ether was removed, placed on a test plate, and incubated for 20 hours in an incubator at 28°C, and the diameter (mm) of the clear zone around the disc was measured.

1.3 Experiment for measuring the number of bacteria in the prototype

The antimicrobial activity test of harmful microorganisms on the prototype was performed by measuring the number of bacteria using LB medium (other harmful bacteria) and BHI (Brain Heart Infusion) medium (mutans bacteria) by adding test bacteria solution to gargle solution.

A mixed strain of S. mutans , E. coli, S. enterica, V. parahaemolyticus, and B. cereus was used, and the number of viable cells was diluted in sterile physiological saline so that the number of viable cells became 1-9.9×10 ⁴ CFU/ml and used as a test bacterial solution. I did.

0.2 ml of the test bacteria solution was added to 20 ml of the gargle stock solution and left for 0, 30, 60, 90, and 120 seconds. In order to cultivate the left bacteria, 1 ml of each of the treated samples was taken in 45-50° C. liquefied LB and BHI medium, put in a liquefied medium, and put in a petri-dish to coagulate. The coagulated petri-dish was cultured at 35° C., caries for 48 hours, and food harmful bacteria for 24 hours to measure the density of the strains grown.

1.4 Oral bad breath gas analysis experiment

A total of 10 Dongshin University college students (3 males and 7 females) were not allowed to consume food for 30 minutes after lunch. In order to collect a sample, one minute before the experiment, the mouth was closed and the nose was made to breathe, and the air in the mouth was gradually filled for 2 minutes using a 500 ml volume air bag (polypropylene material). Then, rinse the mouth with 20 ml of the test product and the gargle solution of rinsterin for 1 minute, spit out the sample, close the mouth, and gradually blow the bad breath gas into the mouth for 2 minutes in increments of 10, 20 and 30 minutes, respectively, and then seal it. The collected air bag was analyzed while being stored at room temperature at 25°C.

For the collected bad breath gas, the methylmercaptane component was quantified using a gas detector (GV-100S, Gastec, Japan). The quantification method was performed by mutually comparing the measured value of the control group treated with rinsing the mouth with sterile water and the measured value of the test product treatment (bad breath inhibition rate (%) = 100 x (final value-initial value) / initial value).

1.5 Sensory evaluation

For sensory evaluation, 20 students from Dongshin University were used as sensory evaluation agents, and the irritation degree, color, taste, oral feel, smell, and preference were performed according to the following 5-point scale method, and each evaluation score was summed. Comparative Example (Control) was carried out with a commercially available listerine (green tea added).

2. Experiment result

2.1 Antibacterial activity test results

The results of the antimicrobial activity against mutans bacteria are shown in Fig. 1 for the mixture, and Fig. 2 for the prototype.

Referring to FIG. 1, whether the mixture sample is a mixture of an ethanol extract or a mixture of essential oil extracts, it can be seen that the antibacterial activity is higher than that of the single sample, and the sample sample generally showed higher antibacterial activity as the amount of sample added increased. In particular, the activities of the prototypes BP1 and B2 were high.

The results of antibacterial activity against other harmful microorganisms are shown in FIGS. 3 to 5. 3 is an antibacterial activity result of the ethanol extract, FIG. 4 is an antibacterial activity result of an essential oil extract, and FIG. 5 is an antibacterial activity result of the prototype.

Most of the samples exhibited antimicrobial activity in general, although there was a difference in degree. In particular, essential oil extracts and prototypes BP1 and BP2 showed high activity.

2.2 Test product bacteria count result

Fig. 6 shows the results of measuring the number of bacteria for the prototype gargle solution. Here, only the prototype BP1 with the highest antibacterial activity was used as the sample. As a result of measuring the number of bacteria, it can be seen that the number of bacteria in the oral cavity before and after gargling was significantly decreased in both LB medium and BHI medium.

2.3 Oral bad breath gas analysis test results

The results of the analysis of oral bad breath gas for the mixture sample are shown in FIG. 7. Again, only the prototype BP1 with the highest antibacterial activity was used as the sample. BP1 had an effect of removing bad breath similar to the positive control, Listerine.

2.4 Sensory evaluation results

The sensory evaluation results are shown in the table below. Sensory evaluation was also performed with only BP1.

There were many opinions that the commercially available listerine (with green tea) had strong irritation, but the developed prototype BP1 was found to have less irritation and good overall preference. It was confirmed that the prototype CM4 was superior to Rinsterine.

Claims (5)

An antibacterial composition against Streptococcus mutans bacteria comprising a cypress leaf extract, a loquat tree leaf extract, or a mixture thereof as an active ingredient.
The method of claim 1,
The composition is a composition, characterized in that the mouthwash composition or toothpaste composition.
A composition for improving dental caries against Streptococcus mutans, comprising a cypress leaf extract, a loquat tree leaf extract, or a mixture thereof as an active ingredient.
The method of claim 3,
The composition is a composition, characterized in that the mouthwash composition or toothpaste composition.
Antibacterial composition against Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus or Bacillus cereus, comprising a cypress leaf extract, a loquat leaf extract or a mixture thereof as an active ingredient.

Images