KR20190110204A - Kyung-ok-go having high acceptability and anti-diabetes activity adding the silk of zea mays and pumpkin - Google Patents

Abstract

The present invention relates to a restorative herb medicine with increased appealability and antidiabetic activity, which is prepared by adding Silk of Zea mays Linne and Cucurbita moschata; and a use of the restorative herb medicine. More specifically, the present invention relates to a restorative herb medicine of a Silk of Zea mays Linne and Cucurbita moschata having improved appealability and strong alpha-amylase and alpha-glucosidase inhibiting activities by adding Silk of Zea mays Linne and Cucurbita moschata to an existing restorative herb medicine comprising Rehmannia glutinosa var. purpurea, Poria cocos, ginseng and honey as main ingredients; and a pharmaceutical composition and health functional food for preventing or treating/ameliorating diabetes comprising the restorative herb medicine of a Silk of Zea mays Linne and Cucurbita moschata. According to the present invention, as being proved through an embodiment of the present specification, a restorative herb medicine composition with increased appealability and excellent antidiabetic activity, which is prepared by adding Silk of Zea mays Linne and Cucurbita moschata: has excellent appealability by adding Silk of Zea mays Linne and Cucurbita moschata; exhibits strong antidiabetic activity by inhibiting alpha-amylase and alpha-glucosidase; does not show hemolytic activity to human red blood cells at all; has excellent thermal stability; and does not exhibit loss in alpha-amylase and alpha-glucosidase inhibiting activities even at an acidic condition of pH 2 and within blood plasma at the same time. Therefore, the restorative herb medicine composition can be easily processed into various forms such as liquid, cream, powder, pill, tablet, and the like, thereby being very useful in pharmaceutical industry and food industry.

Description

KYUNG-OK-GO HAVING HIGH ACCEPTABILITY AND ANTI-DIABETES ACTIVITY ADDING THE SILK OF ZEA MAYS AND PUMPKIN}

The present invention is a silk of Zea mays Linne and Cucurbita The present invention relates to jadeite and its use, which have increased functional and antidiabetic activity prepared by adding moschata ). The present invention relates to jadeite and amber gill jadeite with improved functionality and strong alpha-amylase and alpha-glucosidase inhibitory activity, as well as pharmaceutical compositions and health functional foods for the prevention or treatment / improvement of diabetes comprising the same.

Diabetes, one of the most common diseases in modern people, is a metabolic disease caused by insufficient insulin secretion or lack of normal function. It is characterized by high blood glucose levels and high blood sugar. Cause Diabetes is divided into type 1 and type 2, and type 1 diabetes is caused by the inability to produce insulin at all due to genetic effects, and type 2 diabetes is caused by insulin resistance that decreases insulin function. ) Is known as the cause. In particular, type 2 diabetes is known to have a significant effect on the environmental factors such as high calorie, high fat, high protein diet, lack of exercise, stress due to westernization of diet.

For the treatment of diabetes, insulin injection is essential for type 1 diabetes, lifestyle modification and drug treatment are necessary for type 2 diabetes, and insulin secretagogues (e.g., repaglinide, mitiggle) Amide) and carbohydrate absorption retardants in the small intestine (glucoby: ingredient name-acarbose, Basin ingredient ingredient-volibose) and the like are used.

Gyeongokgo is a solid preparation that consists of ginseng, Bokryeong, Saenghwanghwang, honey, etc. as a constituent herbal medicine. Gyeongokgo is a solid product made by heating raw ginseng juice and honey in white ginseng and baekbokryeong powder and heating it for a long time. According to Dongbogam, 'filling tablets and water, leveling the essence, refreshing the old man, relieving the old man and all kinds of diseases To heal. In addition, the spirit is improved, the five intestines are faithful, and the gray hair is black again, and the missing person comes out again like a walking horse, and if you eat two or three times a day, you are not hungry or thirsty all day long. However, jadeite is a solid product by mixing the raw materials and heated in a hot water bath, the manufacturing method is complicated and has a problem that requires a long time, there is a problem of low palatability due to bitter taste and odor.

Until now, researches related to jadeite have concentrated on the evaluation of useful activity of jadeite itself, the efficacy of Kamigonoe added with natural products, and the effect of fermented jadeite, and its useful physiological activity to enhance immune activity (Lee Ga-soon et al., 2013, Korea Food and Nutrition Division). 42: 335-341), Anti-Fatigue Effect (Jun Ho Kim, 2011, Ph.D., Wonkwang University; Ph.D., 2016, Korean Journal of Pharmacognosy 47: 258-263), Physiological Activity of Kyung-gogo on Hyperglycemia, Hypertension and Inflammation, and Gastric Ulcer Et al., 1994, Korean Journal of Pharmacognosy 25: 51-58), anti-dementia effects (Shin Beom-young, 2011. Kyung Hee University, Ph.D. dissertation), etc., and comparison of the antioxidant effects of various jadeite (Seoyeon Lee, 2008, The Korea journal of herbology 23 : 123-136), and the wool effect of sewage gill jadeite (Do Eun-ju et al., 2011. The Korea journal of herbology 26: 9-14). Recently, beverages using jadeite (Kwon, Se-Wook et al., 2013, Journal of Oriental Physiology and Pathology 27: 233-238) and the manufacture of jadeite vinegar (Seong-Min Park, 2009, Herbal formula science 17: 121-128) have been reported.

On the other hand, patents related to jadeite are patents related to efficient manufacture and absorption of distant jadeite, whose main ingredients are ginseng, bokyeong, raw jihwang, and honey, and jadeite which enhances functionality and palatability by adding various natural products and herbal materials to distant jadeite. Patents related to manufacturing and patents that enhance the palatability and accessibility of manufacturing various beverages, flakes, yokan, chocolate, etc. to facilitate the ingestion of various jadeite can be classified. First, in relation to the manufacture of jadeite and the increase in absorption, Korean Patent No. 10-1725187 discloses [method of preparing jadeite and jadeite prepared accordingly] having small particles for facilitating absorption of jadeite. No. 1441744 discloses a method for producing fermented crypts using microorganisms to increase the amino acid content through fermentation of microorganisms. Next, in connection with the scavenging crypts having increased functionality and palatability, Korean Patent No. 10-1781432 Far-gyeonggogo in the health food for improving hyperlipidemia comprising medicinal crops, including Dansam, Astragalus, hawthorn, malt extract, and Kyungokgo prepared by it; Korean Patent No. 10-1682637, which contains excellent skin improvement effect [Pharmaceutical composition and cheongpi jadeite containing neuronal cell protection and memory improvement effect Functional Food Containing], Addition of Goyang Ginseng to Korean Patent No. 10-1514041 [Gangok Ginseng and its manufacturing method] excellent in palatability and shelf life, Korean Patent No. 10-1198211 No. Method], [jujube gyeongokgo] by adding jujube to the Republic of Korea Patent No. 10-1060309 to increase the functionality of the jujube; Manufacturing method] is disclosed. Lastly, in relation to the patent for manufacturing jadeite into various products having excellent accessibility, Korean Patent No. 10-1716005 discloses the preparation of jadeite powder containing a large amount of active ingredient of powder to increase jadeite efficacy by powdering of components. Method], Republic of Korea Patent No. 10-1454729 [Easily ingested [Method of manufacturing a kyungok flake snack containing a large amount of active ingredient], Republic of Korea Patent No. 10-1404520 [Method of manufacturing chocolate using cheonma gyeonggo and Chocolate manufactured by the above method, Korean Patent No. 10-0286390 [Yangok-type jadeite manufacturing method and its jadeokgo], Republic of Korea Patent No. 10-0354962 can easily be ingested [Gyeongokgo instant tea and its manufacturing method] , Republic of Korea Patent No. 10-1153426 discloses a method for preparing a beverage composition using a jade jade and a beverage composition using the same.

KR 10-1198211 B KR 10-1060309 B KR 10-1016195 B

 Wan-Kyun Hwang et al., 1994, Korean Journal of Pharmacognosy 25: 51-58

The present invention has been made to solve the problems of the prior art as described above, the problem to be solved in the present invention by adding jade water and zucchini to the existing distant jadeite, jade water and increased functional and antidiabetic activity It is to provide a new jadeite composition with amber added. In addition, the present invention is to provide a pharmaceutical composition for preventing or treating / improvement of diabetes and health functional food comprising the jadeite composition of the present invention.

In order to solve the above problems, the present invention with respect to 100 parts by weight of ginseng, 80 to 120 parts by weight of Baekbokyeong, 350 to 450 parts by weight of raw turmeric, 350 to 450 parts by weight of honey, 1,500 to 1,800 parts by weight of jade and pumpkin 600 It provides jade water, amber-flavored light jadeite prepared by mixing, heat extraction, filtration and concentration.

The present invention also provides a pharmaceutical composition for the prevention or treatment of diabetes, comprising the chalcedony amber gourd amber jadeite of the present invention as an active ingredient.

The active ingredient is preferably a hexene fraction, an ethyl acetate fraction, a water residue, or a mixture thereof obtained by sequentially dividing oxime water and zucchini-grilled jadeite with hexene and ethyl acetate.

Moreover, this invention provides the health functional food for the prevention or improvement of diabetes containing the chalcedony water and pumpkin kamikaze jade of this invention.

The active ingredient is preferably a hexene fraction, an ethyl acetate fraction, a water residue, or a mixture thereof obtained by sequentially dividing oxime water and zucchini-grilled jadeite with hexene and ethyl acetate.

As the functionality of the present invention is increased, jadeite water and zucchini-added jadeite composition excellent in anti-diabetic activity, as demonstrated through the examples of the present specification, not only jade water and pumpkin are added, but also excellent in functionality. It exhibits strong antidiabetic activity by inhibiting, alpha-amylase and alpha-glucosidase, shows no hemolytic activity against human erythrocytes, and has excellent thermal stability, alpha-amylase even in acidic conditions and plasma of pH 2 And since there is no loss of alpha-glucosidase inhibitory activity, it can be easily processed into various forms such as liquid, cream, powder, pills, tablets, etc. can be very useful in the pharmaceutical industry and food industry.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The inventors of the present invention can easily ingest by increasing the functionality, while increasing the alpha-amylase and alpha-glucosidase inhibitory activity to produce anti-diabetic activity to produce jadeite, which can benefit health, various natural products It was confirmed that during the preparation of Kami jadeite added with the addition, jadeite produced by adding jade water and pumpkin was very excellent in the increase of sensory function and antidiabetic activity. The inventors of the present invention, by adding various ratios of jade water and pumpkin to the existing jadeite to determine the addition ratio excellent functionality, and then compared with the conventional jadeite to evaluate the physicochemical properties, useful component content analysis and antidiabetic activity It was. The jadeite and zucchini-added jadeite have no hemolytic activity against human erythrocytes, but have excellent thermal stability and acid stability. To improve the pharmaceutical composition and health functional food.

Therefore, the present invention is mixed with 100 to 100 parts by weight of ginseng, 80 to 120 parts by weight of Baekbokyeong, 350 to 450 parts by weight of raw sulfur, 350 to 450 parts by weight of honey, 1,500 to 1,800 parts by weight of jade and 600 to 800 parts by weight of pumpkin Provided are jade water, pumpkin amber jadeite composition prepared by extraction, filtration and concentration.

It is preferable to heat-extract 24 to 48 hours at 95 degreeC, and it is preferable to concentrate the final sugar to 25-30 brix in order to improve palatability.

As a preferred embodiment, the preparation of the jade water-pumpkin kerchief jadeite composition of the present invention is 3% by weight of ginseng powder, 3% by weight of Baekbokyeong powder, 12% by weight of raw sulfur powder, 12% by weight of honey, 49% by weight of jade water and 21% of pumpkin A mixture containing% may be prepared, and the mixture may be heated and extracted at 95 ° C. for 24 to 48 hours, filtered, and then concentrated to 26 brix. The concentrate was much more functional than conventional jadeite, and the bitter and sour taste was hardly felt. In addition, the anti-diabetic activity was evaluated as alpha-amylase and alpha-glucosidase inhibitory activity in the jade water-pumpkin gypsy jadeite composition of the present invention prepared as described above. It was confirmed that the anti-diabetic activity is stronger than that of jadeite.

The jade water-pump amber light jadeite composition of the present invention can also be prepared as an active fraction by an additional organic solvent fraction in a concentrated state. In a preferred embodiment, the jade water amber zucchini kerchief composition of the present invention can be fractionated as a water residue after hexene fraction, ethyl acetate fraction and organic solvent fraction by performing sequential organic solvent fractionation of hexene and ethyl acetate.

The jade water-pumpkin sapphire jadeite composition prepared from the above preferred embodiment exhibits a similar sugar content to the existing jadeite, but has an acidity of 0.15%, which is lower than 0.97-1.0% of the existing jadeite. In addition, the conventional jadeite inhibits alpha-amylase and alpha-glucosidase by 0.2% and -1.5% at the final concentration of 1.3 mg / ml, respectively, and shows negligible antidiabetic activity. In the final concentration of 1.3mg / ml, alpha-amylase and alpha-glucosidase were inhibited by 4.1% and 8.2%, respectively, and showed excellent antidiabetic activity. In particular, the hexene fraction, ethyl acetate fraction and water residue of the jade water-pumpkin jadeite composition inhibited 2.4%, 1.7% and 4.6% of alpha-amylase at 0.5 mg / ml concentration, respectively. 16.3%, 18.6% and 20.7% were inhibited. It was determined that the anti-diabetic active ingredient of the present invention was not dependent on jade water, considering that the lukewarm hydrothermal extract showed less than 0.1% alpha-amylase and alpha-glucosidase inhibitory activity at 0.5 mg / ml. . In addition, it was confirmed that the jade water-pump amber light jadeite composition of the present invention does not exhibit human erythrocyte hemolytic activity, and shows stability during acid treatment, heat treatment, and plasma treatment.

Accordingly, the present invention provides a pharmaceutical composition for preventing or treating / improvement of diabetes and a health functional food containing the jade water, pumpkin kamikaze jadeite composition of the present invention as an active ingredient.

The active ingredient may be a hexene fraction, an ethyl acetate fraction, a water residue, or a mixture thereof obtained by sequentially dividing oxime water and zucchini-grilled jadeite with hexene and ethyl acetate.

The jade water, zucchini-flavored jadeite and its active fractions contained in the composition of the present invention may be prepared into a powder through a conventional powdering process such as vacuum drying and freeze drying or spray drying. The antidiabetic active ingredient of jade water, pumpkin amber jadeite and its active fractions is not degraded by various degrading enzymes in plasma, and is maintained at 100 ° C. heat treatment and pH 2 in the human stomach.

The jade water, amber gill jadeite and its active fractions of the present invention exhibit potent alpha-amylase and alpha-glucosidase inhibitory activity, which leads to type 1 diabetes, type 2 diabetes or diabetic retinopathy and diabetes mellitus. It can be used as a material for pharmaceutical compositions and health functional foods related to the prevention and treatment / improvement of diabetic complications such as nephropathy.

In a preferred embodiment, the chalcedony amber amber amber jadeite and its active fractions of the present invention can be applied as a use of a pharmaceutical composition for the prevention or treatment of diabetes.

The pharmaceutical composition may be formulated in various forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, injections of sterile injectable solutions, etc. It may be used orally or may be administered through various routes including intravenous, intraperitoneal, subcutaneous, rectal, topical, and the like. Such pharmaceutical compositions may further include carriers, excipients or diluents, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil Etc. can be mentioned. In addition, the pharmaceutical composition of the present invention may further include a filler, an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, a preservative, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level means the type, severity, activity of the drug, Sensitivity to drug, time of administration, route of administration and rate of release, duration of treatment, factors including concurrent use of drugs, and other factors well known in the medical arts.

The pharmaceutical compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered as single or multiple doses. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect in a minimum amount without side effects, which can be easily determined by those skilled in the art.

The effective amount of the component having antidiabetic activity in the pharmaceutical composition of the present invention may vary depending on the age, sex and weight of the patient, and generally 1 to 5,000 mg, preferably 100 to 3,000 mg per body weight daily or every other day. It may be administered or divided into 1 to 3 times a day. However, the dosage may be increased or decreased depending on the route of administration, the severity of the disease, sex, weight, age, etc., and the above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration can be expected, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intra-cerebroventricular injections. As used herein, "administration" means providing a patient with any substance by any suitable method, wherein the route of administration of the pharmaceutical composition of the present invention is oral or parenteral via all common routes as long as the target tissue can be reached. Oral administration. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to a target cell.

"Subject" in the present invention is not particularly limited, but includes, for example, humans, monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, rats, rabbits or guinea pigs. And preferably mammals, and more preferably humans.

In a preferred embodiment, the chalcedony amber amber amber jadeite and its active fractions of the present invention can be applied as a use of a dietary supplement for the prevention or improvement of diabetes.

Examples of the food containing the active ingredient excellent in antidiabetic activity of the present invention include various foods, beverages, gums, teas, vitamin complexes, health supplements, and the like, which are powders, granules, tablets, capsules, or beverages. Available in form.

The active ingredient of the present invention can generally be added at 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 10g, preferably 0.3 to 1g based on 100 ml.

In addition to containing the compound as an essential ingredient in the indicated ratios, the health functional food of the present invention may contain food-acceptable food supplement additives such as natural carbohydrates and various flavoring agents as additional ingredients. Examples of the natural carbohydrates include conventional sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, tau martin, rebaudioside A, glycyrgin, saccharin, aspartame and the like can be used. The proportion of the flavoring agent is generally used from about 1 to 20 g, preferably from about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals, synthetic flavors and natural flavoring agents, colorants and neutralizing agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids Thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like.

In addition, the health functional food of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage, vegetable beverage and the like. These components can be used independently or in combination. The proportion of such additives is generally selected from the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are only preferred embodiments of the present invention, and the scope of the present invention is not limited to the following examples.

[ Example ]

Example  One: Jade Water Pumpkin kamikaze Jadeite  Preparation and Analysis of Physicochemical Properties and Useful Components

3% by weight of ginseng powder, 3% by weight of Baekbokryeong powder, 12% by weight of raw sulfur powder, 49% by weight of jade water, 21% by weight of pumpkin, and 12% of honey, which were heated and extracted at 95 ° C. for 48 hours and filtered. The results of physicochemical properties, useful component analysis and sensory evaluation of Okmisu-zucchini jadeite jadeite prepared by concentrating to 26 briquettes are shown in Table 1, Table 2 and Table 3, respectively. At this time, a commercial jadeite was used as a control.

As a result, as shown in Table 1, the jade water-pumpkin kerbstone of the present invention showed an acidity of about 1/6 compared with commercial jadeite, and showed low brightness, yellowness, and high redness. However, the overall color difference was similar to that of commercial jadeite. At this time, the color difference analysis was measured using a Hunter Color Difference meter (Super color SP-80 Colormeter, Tokyo Denshoku Co., Japan), brightness (white 100 ~ 0 black), redness (red 100 ~ -80 green), Yellowness (yellow 70-80 black) was measured. At this time, the chromaticity of the standard whiteboard was set to L. 92.44, a value of -0.06, and b value of 1.35. The average value was obtained by measuring three times per sample, and the color difference (△ E) was expressed by the following equation. Calculated by

The total polyphenols, total flavonoids, total sugars and reducing sugars contents of commercial jadeite and chalcedony amber amber jadeite were measured. The total polyphenol content was added to 400μl of the extracted sample solution, 50μl of Folin-ciocalteau, 100μl of Na ₂ CO ₃ saturated solution, and left at room temperature for 1 hour and absorbance at 725nm. Tannic acid was used as the standard reagent. For the total flavonoid content, each sample was extracted by stirring for 18 hours with methanol, 4 ml of 90% diethylene glycol was added to 400 µl of the filtered extract sample, 40 µl of 1N NaOH was added thereto, and the absorbance was measured at 420 nm after 1 hour of reaction at 37 ° C. Rutin was used as a standard reagent. Reducing sugar was determined by DNS method and total sugar was determined by phenol-sulfuric acid method.

As a result, as shown in Table 2, the total polyphenol content was 1.3 times higher than that of commercial jadeite, but the total flavonoid content was 5.7 times higher than that of commercial jadeite. It also showed a lower reducing sugar content.

On the other hand, as a result of evaluating the functionalities of the jade and zucchini jadeite and commercial jadeite in the present invention with taste, aroma, thirst, and overall preference, it was confirmed that the sensory ability was superior to that of commercial jadeite, Women in their twenties also could feel free to drink (Table 3).

Example  2: Jade Water Pumpkin kamikaze Jadeite  And objections Organic solvent Fraction Antidiabetic  Active evaluation

The antidiabetic activity of jade water and pumpkin amber jadeite jadeite prepared in Example 1 was shown by evaluation of in-vitro α-amylase inhibitory activity and α-glucosidase inhibitory activity. First, α-amylase inhibitory activity was used by modifying the method reported by Lim et al. (Li, CS et al., 2005. J. Kor. Soc. Appl. Biol. Chem. 48: 103-108). 2.5 μl of sample and 25 μl of α-amylase (0.25 U / mL) diluted with 50 mM phosphate buffer (pH 6.8) were mixed and preincubated at 37 ° C. for 10 minutes, followed by 25 μl of 0.5% soluble starch (Samchun Chemicals Co., Korea). The reaction was carried out at 37 ° C. for 10 minutes. Thereafter, 25 μl of 2N acetic acid was added to stop the reaction, and 10 μl of 0.75% KI / I ₂ solution was added to the reaction solution. The color solution was measured for absorbance at 540 nm, and each experiment was repeated three times, and the average value was calculated. The inhibition rate was calculated by the following equation.

Inhibition rate ( % ) = Sample Addition  Enzyme activity / Control Addition  Enzyme Activity x 100

Meanwhile, α-glucosidase inhibitory activity was evaluated using pNPG (p-nitrophenol glucoside; Sigma Co., USA), and α-glucosidase (0.25U diluted with 2.5 μl of ginger extract sample and 50 mM Sodium acetate buffer (pH 5.6)). / 25) 25μl was mixed and preincubated for 10 minutes at 37 ℃, 25μl of 1mM pNPG solution was added and reacted for 10 minutes at 60 ℃. Thereafter, 25 μl of 1 M NaOH was added to stop the reaction, and the inhibition rate was calculated by measuring the absorbance at 405 nm.

Inhibition rate ( % ) = (1- (sample Addition  Enzyme activity / Control Addition  Enzyme activity)] x 100

As a result, as shown in Table 4, acarbose used in the clinical treatment of diabetes showed potent α-amylase inhibitory activity and α-glucosidase inhibitory activity in a concentration-dependent manner. Commercial jadeite did not have α-amylase and α-glucosidase inhibitory activity, while jade and zucchini gygogo showed excellent α-amylase and α-glucosidase inhibitory activity. Accordingly, hexene fraction, ethyl acetate fraction and water residue were prepared by sequential organic solvent fractionation using hexene and ethyl acetate in jade water and zucchini amber jadeite, and the antidiabetic activity evaluation results of the fractions are shown in Table 5. It was.

As a result, potent α-glucosidase inhibitory activity was confirmed in all hexene fractions, ethyl acetate fractions and water residues of jade water and zucchini kelp. The inhibitory activity of these fractions was more potent at higher concentrations and showed concentration-dependent inhibitory activity. Accordingly, it was confirmed that jade water and pumpkin amber jadeite and its active fractions can be used for the prevention and treatment of type 2 diabetes.

Example  3: Jade Water Pumpkin kamikaze Jadeite  And its activity Fraction  Plasma, Acid and Thermal Stability Assessment

Thermal stability and acid stability against α-glucosidase inhibitory activity were confirmed in the jade water, pumpkin-grill jadeite and active fractions and water residues obtained in Examples 1 and 2 above. They did not show significant decrease in α-glucosidase inhibitory activity after 1 hour heat treatment at 100 ° C and 1 hour treatment at pH 2 (0.01 M HCl). Therefore, it is determined that the jade water, pumpkin kamikaze jadeite and its active fractions of the present invention will maintain antidiabetic activity even in the general food processing process and digestive absorption step.

Example  4: Jade Water Pumpkin kamikaze Jadeite  Human Erythrocyte Hemolytic Activity Assessment

Okmisu and pumpkin have long been used as food beverages and herbal ingredients, no side effects are known. Therefore, jade water and pumpkin amber jadeite are unlikely to have any specific toxicity. Human erythrocyte hemolytic activity was assessed to assess the potential acute toxicity of jade water and pumpkin amber jadeite, and the results are shown in Table 6. The hemolytic activity was evaluated according to the previous report (Son Ho Yong, 2014, Korean J. Microbiol. Biotechnol. 42: 285 ~ 292), and 100 μl of human red blood cells washed three times with PBS was added to a 96-well microplate. 100 μl of various concentrations of the sample solution were added, followed by reaction at 37 ° C. for 30 minutes. After that, the reaction solution was centrifuged for 10 minutes (1,500 rpm) to transfer 100 μl of the supernatant to a new microtiter plate. Measured. DMSO (2%) was used as a solvent control of the sample, and Triton X-100 (1 mg / ml) was used as an experimental control for red blood cell hemolysis. Hemolytic activity was calculated using the following formula.

( % ) Hemolysis  = [( Abs .S- Abs .C) / ( Abs .T- Abs .C)] × 100

Abs. S: absorbance of the sample addition port,

Abs. C: absorbance of DMSO house furniture,

Abs . T: Triton X-100 Addition of  Absorbance.

First, DMSO and distilled water used as a control did not have erythrocyte hemolytic activity, Triton X-100 was confirmed that 100% hemolysis of red blood cells at a concentration of 1mg / ml. The anticancer drug emoterasin B, which has been reported to be acutely toxic, showed more than 95% erythrocyte hemolytic activity even at 0.0125 mg / ml. On the other hand, jade water and amber amber jadeite was not shown hemolytic activity at 130mg / ml concentration as in commercial jadeite, and fractions thereof were not recognized hemolytic activity.

Claims (4)

100 to 100 parts by weight of ginseng, 80 to 120 parts by weight of Baekbokryeong, 350 to 450 parts by weight of raw turmeric, 350 to 450 parts by weight of honey, 1,500 to 1,800 parts by weight of jade water and 600 to 800 parts by weight of pumpkin, mixed, extracted, filtered and concentrated Jade water, pumpkin kamikaze jade coke produced by A pharmaceutical composition for the prophylaxis or treatment of diabetes, comprising the jade water, zucchini, kamikaze, and jadeite as described in claim 1 as an active ingredient. The pharmaceutical composition according to claim 2, wherein the active ingredient is a hexene fraction, an ethyl acetate fraction, a water residue, or a mixture thereof obtained by sequentially dividing oxime water and pumpkin amber jadeite into hexene and ethyl acetate. Composition. A dietary supplement for preventing or improving diabetes comprising the active ingredient according to claim 2 or 3.