KR20190088740A - Antioxidant and anti-Obesity composition comprising Psyllium Husk and Kaempferia parviflora, formulatiom using the composition and preparing method thereof - Google Patents

Abstract

The present invention relates to an antioxidant and anti-obesity composition comprising 50 to 70 parts by weight of powdered tea powder and 5 to 20 parts by weight of a black ginger powder, an antioxidant and anti-obesity agent granulated with the composition and a method for producing the same, Is excellent in antioxidative effect as confirmed by polyphenol content, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power when it is prepared by using the natural tea component powder and black ginger powder, , Body mass index, body fat, body fat percentage, and abdominal fat ratio.

Description

[0001] The present invention relates to an antioxidant and antiobesity composition containing an antioxidant and black ginger, a preparation using the antioxidant and an anti-obesity composition, and a method for preparing the antioxidant and anti-obesity composition,

The present invention relates to an antioxidant and anti-obesity composition containing tea tramp blood and black ginger as active ingredients, an antioxidant and an anti-obesity agent granulated with the composition, and a process for producing the same.

Since the life expectancy of human beings has been lengthened and the quality of life has been rapidly improved due to the development of economic development and medical technology in the world as well as in Korea, there is a urgent need for an aggressive wellbeing response strategy corresponding to this. Especially, it is necessary to develop effective and safe related health functional foods in the dimension of accelerating aging, protection of the public health due to the occurrence of adult diseases, and reduction of the government and individual medical expenses.

Cardiovascular diseases such as heart attack, hypertension, arteriosclerosis and stroke account for 30% of the total mortality worldwide. In particular, obesity, diabetes, hypertension, atherosclerosis, heart disease, and stroke are seen as different diseases, but they are understood as a syndrome with a common cause of insulin resistance due to increased body fat.

Obesity is one of the most common malnutrition disorders worldwide. According to WHO statistics, 250 million people are currently classified as obese, and after 20 years about 300 million people are expected to suffer from obesity.

Obesity is a major problem in obesity-related diseases caused by obesity rather than obesity itself. In general, obese people are at risk of developing atherosclerosis 4 times, diabetes 2 times, hypertension 3 times, and heart disease 3 times higher than normal people. The higher the degree of obesity, the higher the prevalence of metabolic syndrome such as diabetes, cholelithiasis, hypertension, heart disease, stroke, etc., resulting in a great economic and social loss caused by obesity.

According to epidemiological studies conducted in the US, about 280,000 deaths from obesity-related illnesses in 1991, about 80% of them were obese at a BMI> 30, and about 120 trillion won a year were spent on treating obesity-related diseases Are reported.

Currently, a variety of products related to constipation improvement and body fat reduction have been developed at home and abroad, mainly based on dietary fiber.

Korean Patent Registration No. 10-0504124 Korean Patent Registration No. 10-0562467 Korean Patent Registration No. 10-1488612 Korean Patent Registration No. 10-1672199

It is an object of the present invention to provide an antioxidant and anti-obesity composition which is excellent in antioxidative effect and excellent in body weight and body fat loss effect by using tea root oil and black ginger which are natural ingredients.

In order to achieve the above object,

The present invention provides an antioxidant and anti-obesity composition comprising 50 to 70 parts by weight of powdered tea powder and 5 to 20 parts by weight of black ginger powder. The composition preferably contains 55 to 65 parts by weight of the tea powder powder and 7 to 14 parts by weight of the black ginger powder. In the composition, it is preferable that the content of the dietary fiber in the powdery powder is 80% or more.

The composition may further comprise 0.5 to 20 parts by weight of one or more additional components selected from green tea extract powder, aloe extract powder, prune concentrated powder and probiotics.

The composition may further comprise 0.5-20 parts by weight of one or more additives selected from powdered crystalline glucose, amylase, vegetable protease, enzyme-treated stevia, vegetable cream powder and maltodextrin.

The present invention also provides granular antioxidant and anti-obesity preparations in which the antioxidant and anti-obesity composition is formulated in the form of granules.

In addition,

(a) an active ingredient comprising 50 to 70 parts by weight of the powdery powder and 5 to 20 parts by weight of black ginger powder; (b) a green tea extract powder; Aloe extract powder; Prune-concentrated powder; 0.5 to 20 parts by weight of one or more additional components selected from probiotics and (c) one or more additives selected from powdered crystalline glucose, amylase, vegetable protease, enzyme-treated stevia, vegetable cream powder and maltodextrin 0.5 To 20 parts by weight of a raw material;

Mixing each of the homogenized raw materials;

Adding 1 to 100 parts by weight of drinking water per 100 parts by weight of the mixture to the mixture of the raw materials;

Granulating a mixture of the raw material and the drinking water; And

And drying the granules so that the water content is not more than 10% at 60 占 占 폚. The present invention also provides a method for producing granular antioxidant and anti-obesity agents.

The antioxidant and anti-obesity composition of the present invention has excellent antioxidative effect as confirmed by polyphenol content, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power, by using a natural tea component powder and black ginger powder , And the anti-obesity effect confirmed by the decrease in body weight, body mass index, body fat, body fat percentage and abdominal fat percentage when the human body is ingested is excellent. Also, because it is harmless to human body by using natural ingredients, it can be useful as a dietary supplement.

Figure 1 is a photograph of the granular antioxidant and anti-obesity agent of the present invention.

The antioxidant and anti-obesity composition of the present invention comprises 50 to 70 parts by weight of powdery powder and 5 to 20 parts by weight of black ginger powder. Preferably 55 to 65 parts by weight of the tea powder powder and 7 to 14 parts by weight of the black ginger powder.

Psyllium Husk is a bark of seeds of plantain, which grows naturally throughout the country. It is effective for the removal of the intestinal tract of the intestinal tract because it contains a large amount of arabinoxylan-polysaccharide which forms mucus, which is excellent in moisture retention. This is because hemicellulose half-water soluble fiber in the plantain It absorbs much moisture more than 40 times its own weight, and it is known that it expands in volume to excrete impurities and succulents attached to the barrier and acts on the intestinal wall to promote peristaltic movement of the intestines. The major components of tea tram blooms are dietary fibrinolytic substances such as mucus, fat, aucubin, galactan, polysaccharide and succinic acid, which are mostly hydrolyzed by in vivo digestive enzymes. It has been attracting attention as a low-calorie food material because it is used by intestinal bacteria and has a low energy calorie. The effect of tea tramp blood was evaluated as a decrease in serum total cholesterol concentration, a decrease in LDL cholesterol concentration, a decrease in neutral lipid concentration, a decline in experimental hepatotoxicity caused by carbon tetrachloride, a decrease in total cholesterol concentration in type 2 diabetic patients, Have been reported. In the composition of the present invention, it is preferable to use a powder having a dietary fiber content of 80% or more.

Black ginger ( Kaempferia parviflora ) belongs to the ginger (Zingiberaceae) and Turmeric, also known as KraChaDum in Thai. It is known that there are about 60 species in the world. Plants cultivated mainly in Laos, Thailand and Indochina Peninsula in Southeast Asia are being studied as traditional medicinal materials. Roots are used as traditional traditional medicines in Southeast Asia . Black ginger is known to exhibit various physiological activities such as antioxidant, antiinflammatory, gastrointestinal protection, male sexual function improvement, anti-malaria, antiviral, antibacterial, anti-cancer, antiallergic, anti-obesity, pain relief such as abdominal discomfort relief. In particular, in the ob / ob obesity animal model, the standard extract of black ginger (KPE) and its functional component DMF (5,7-dimethoxyflavone) did not affect the appetite of the experimental animals, (AMP-activated protein kinase) was activated in epididymal adipose tissue to inhibit the expression of lipogenesis-related transcription factors and lipid-related enzymes and to reduce the symptoms of cholesterol and nonalcoholic fatty liver . It has also been reported to significantly increase muscle fiber size, muscle volume and muscle weight, thereby improving muscle function such as endurance and strength. The administration of black ginger extract to 27 mice significantly reduced body weight and visceral fat, and the activity of brown adipose tissue in healthy subjects at the time of ingestion of black ginger ethanol extract (100 mg / day) To increase total energy consumption. The mechanism of obesity inhibition of black ginger extract was found to promote the energy metabolism by the activation of brown adipose tissue by increasing the expression of UCP1 (uncoupling protein 1) in brown adipose tissue. In addition, studies have shown that 100 ㎍ / ㎖ of black ginger extract has a 5-fold higher activity of SIRT1 than that of resveratrol. SIRT1 enzyme is a long-lived gene that is associated with senescence, diabetes, obesity, It is known as a gene to delay symptoms. In addition, the ethanol extract of black ginger significantly inhibited stress-induced gastric ulcer formation in a rat model, indicating that methoxyflavones may be effective in humans, and cholinesterase (methoxyflavones) ), Which is a potential therapeutic drug for Alzheimer's.

The main ingredients of black ginger are 5,7-dimethoxyflavone, 5,7,4'-trimethoxyflavone, 5,7-dimethoxyflavone, , 5,3'4-tetramethoxyflavone, 5-hydroxy-7-methoxyflavone, 5-hydroxynaphthoic acid, Hydroxy-7,7-dimethoxyflavone, 5-hydroxy-3,7-dimethoxyflavone, 5-hydroxy- 5-hydroxy-3,7,4'-trimethoxyflavone, 5-hydroxy-3,7,3 ', 4'-tetramethoxy flavone (5- hydroxy-3,7,3 ', 4'-tetramethoxyflavone), 3,5,7-trimethoxyflavone, 3,5,7,4'-tetramethoxy flavone (3 , 5,7,4'-tetramethoxyflavone), 3,5,7,3 ', 4'-pentamethoxyflavone (3,5,7,3', 4'-pentamethoxyflavone) have been reported.

In animal studies, the effective dose of black ginger is 250-500 mg / kg body weight, and toxicity data are reported to be safe up to 500 mg dose per kg of body weight. In healthy elderly subjects, 90 mg / When the black ginger extract was taken at a dose of 180 mg / day for 12 weeks, the athlete showed no side effects and was reported to improve the aerobic capacity of soccer players.

In the composition of the present invention, it is more preferable to use Thai ginger (liquor).

The composition of the present invention may further comprise 0.5 to 20 parts by weight of one or more additional components selected from green tea extract powder, aloe extract powder, prune concentrated powder and probiotics.

The green tea extract may exhibit the known effect of green tea such as antioxidative action in the present composition, and particularly acts to decompose body fat. The green tea extract powder is more preferably contained in the composition in an amount of 2 to 8 parts by weight.

The aloe extracts exhibit the known effects of aloe in the present composition and can particularly aid in bowel action. The aloe extract powder is more preferably contained in the composition in an amount of 0.2 to 1 part by weight. Aloe is a perennial herbaceous tropical plant belonging to the genus Aloinease of the Liliaceae . To date, about 200 active ingredients of aloe have been known, of which anthraquinones and polysaccharides are the major components. Anthraquinones are yellow sap which are present in flow cells in the ducts located just below the aloe skin. Aloin and aloeemodin are the main components, and they have a bitter taste. They stimulate secretion of saliva and gastric juice, And has been reported to have an effect of improving the constipation and antimicrobial action. The polysaccharide Aloe Borane A has a hypoglycemic effect, and Aloe Vera gel is said to neutralize the acid and give a feeling of satiety. It is also expected that antioxidative action of aloe's anthraquinones and polyphenol materials is due to the hydroxyl group. Aloe phenolic acid esters are also known to have a very strong antioxidant activity. The most widely used aloe species are aloe vera and aloe avoresense, and aloe avoresense is limited to foods due to bitter taste and anthraquinone substances, but aloe vera is widely used as juice or gel type health functional food It is most widely used in pharmaceuticals and cosmetics.

Prunes exhibit the known effect of prunes in the present compositions, and can especially aid bowel movements and improve the taste of the present compositions as taste crystals. The prune-concentrated powder is more preferably contained in the composition in an amount of 1 to 3 parts by weight.

Probiotics refers to probiotics capable of ameliorating intestinal bacteria and refers to substances that promote the proliferation of intestinal bacteria (ovarian-resistant carbohydrates such as oligosaccharides), and their mixture is called synbiotics. More preferably, the probiotics in the present composition may be contained in an amount of 0.2 to 1 part by weight. The probiotics contained in the present composition are preferably selected from the group consisting of Streptococcus thermophilus, Lactobacillus platarium, Lactobacillus paracasei, Bifidobacterium long sword, Lactobacillus lambatus, Lactobacillus acidophilus, Lactobacillus casei , Bifidobacterium breve, Lactobacillus perfumantum, Bifidobacterium bifidum, Lactobacillus debruecki sspcbulgaricus. Lactobacillus reuteri, and the like. The probiotics particularly preferably comprise Lactobacillus platarium and Lactobacillus acidophilus.

Lactobacillus Acidophilus is an indigenous dominant fungus in the intestine of a healthy person. It is resistant to low pH, bile acid and lysozyme in the gastrointestinal tract and can adsorb to the intestinal epithelium. It has excellent survival and fixability in the digestive tract, and grows at a high level especially at the end of the ileum. Lactobacillus ashidophilus not only maintains beneficial normal microflora by controlling intestinal colonization and growth and metabolic activity of harmful microorganisms in the small intestine, but also reduces the chance of temporarily colonizing infected intestinal pathogens in the intestine, especially the small intestine.

Lactobacillus plantarum inhibits intestinal pathogens, reduces intestinal permeability, reduces abdominal distension, strengthens the immune system, reduces the frequency and severity of flu and flu, suppresses fungal infections, inhibits pancreatic hematopoietic infections, immunologic stimulation of HIV children , Reducing the risk of postoperative infection, relieving symptoms of trauma patients, inhibiting burn infection (locally), accelerating healing of the sun, reducing the risk of pneumonia, reducing the inflammatory response, reducing the risk of kidney stones, and reducing blood pressure.

The composition of the present invention may further comprise 0.5 to 20 parts by weight of one or more additives selected from powdered crystalline glucose, amylase, vegetable protease, enzyme-treated stevia, vegetable cream powder and maltodextrin.

Stevia (Stevia rebaudian bertoni) is a chrysanthemum that grows wild in Brazil and Paraguay and is a sweet herb. Stevia is mainly used in Japan, Korea, China and South America. It has been used for various foods such as confectionery, pickles and seafood including beverages for about 10 years as a sweetener. Stevia extract is widely used as a dietary supplement in the United States . The main component of stevia is stevioside and rebaudioside A, C, D, E with steviol as glycosides, and ducoloside A is present in trace amounts. Stevioside is a sweetener with low calorie value and is recognized as a sweetener for improving sucrose and sweeteners. Since 1952, the US National Institute of Health (NIH) has been working on a new anti-arthritic raw material, And is used for diabetes. Dried Stevia leaves contain about 5-10 weight percent stevioside, and the sweetness component is said to be 200-300 times more sweet than sugar. In addition to stevioside, stevia extract also contains various polyphenols, which are found to have high antioxidant ability that is more than 20 times that of green tea. In addition, it has been shown that the high fat diet inhibits weight gain, weight loss, , Inhibits lipid accumulation in tissues, increases β-oxidation, and decreases the accumulation of fat in the body. Enzymatic treatment Stevia is obtained by adding glucose to a stevia extract using α-glucosyltransferase or the like, and its component is α-glucosyl stevioside. When it is converted into a dried product, it is considered that the stevia glycoside is more than 80.0% And not more than 15.0% of unreacted steviol glycosides.

The composition of the present invention can be prepared in granular form in antioxidant and anti-obesity preparations.

First, 56 to 60 parts by weight of the powdery green tea powder, 5 parts by weight of the black ginger powder and 21 to 27 parts by weight of the other ingredients are sieved, homogenized and mixed. At this time, it is more preferable to use a # 40 sieve.

1 to 100 parts by weight of drinking water per 100 parts by weight of the mixture is added to the mixture. In this case, since the drinking water is used to make the mixture into a bamboo shape and to be hipped, the amount necessary for making the bamboo is enough, and the drinking water is preferably water that can be used as drinking water.

The mixture is granulated. At this time, granulation is preferably carried out in a granulator using a mesh standard of 1.5 mm / # 12 mesh.

After granulation, the granules are dried at 60 ± 2 ° C to a moisture content of 10% or less to prepare granular antioxidant and anti-obesity preparations.

The antioxidant and anti-obesity composition of the present invention as described above is excellent in antioxidative effect as confirmed by polyphenol content, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power, and having a neutral pH and slightly reddish brown , Body weight, body mass index, body fat, body fat percentage, and abdominal fat ratio when administered to human body.

[ Example ]

Hereinafter, the present invention will be described in more detail with reference to examples. The following examples illustrate the invention and are not to be construed as limiting the scope of the invention.

< Example  1>

The tea flour powder was purchased from India with 88% dietary fiber content, and black ginger powder was purchased from Thailand.

Amylase 0.5 g, vegetable protease 0.5 g, prune-concentrated powder 2 g, enzyme-treated stevia 1 g, probiotics (12 g), black ginger powder 10 g, green tea extract powder 5 g, aloe extract powder 5 g, powdered crystalline glucose 2 g, ), 4.5 g of vegetable cream powder, and 8 g of maltodextrin were mixed in a # 40 sieve and mixed in a mixer at 30 rpm for 10 minutes to prepare an antioxidant and anti-obesity composition. The probiotics (12 kinds) include Streptococcus thermophiles, Lactibacillus plantarum, Lactobaillus paracasei, Bifidobacterium longum Lactobacillus rtamnosus, Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium breve, Lactobacillus fermentum, Lactobacillus debruecki sspcbulgaricus, Lactobacillus reuteri, Bifidobacterium bifidum.

< Example  2>

To the composition of Example 1 was added water And the mixture was uniformly mixed for 10 minutes.

The mixture of the composition and the drinking water was granulated in a granulator using a mesh size of 1.5 mm / # 12 mesh. Granulated and dried at 60 ± 2 ° C to a moisture content of 10% or less to prepare granular antioxidant and anti-obesity preparations.

FIG. 1 shows photographs of the prepared granular antioxidant and anti-obesity preparations.

[ Experimental Example ]

Preparation of experimental sample

In Experimental Example 1, 10 times of distilled water was added to the antioxidant and anti-obesity composition prepared in Example 1 and used as an experimental sample. In Experimental Examples 2 to 7, the antioxidant and anti-obesity compositions prepared in Example 1 were dissolved in methanol and then filtered to be used as an experimental sample.

< Experimental Example  1>

Check pH and color

The pH and chromaticity of the antioxidant and anti-obesity compositions of the present invention were determined.

The pH of the test sample was 7.45 ± 0.02.

The chromaticity of the test sample was 33.44 ± 0.59, 5.88 ± 0.11, and 18.14 ± 0.50, respectively. The color of the sample was slightly reddish brown (See Fig. 1). These colors are considered to be the result of blending the violet of black ginger with the color of other materials.

< Experimental Example  2>

Polyphenol compound content

The polyphenol compound is a secondary metabolite widely distributed in the vegetable field. It is a generic term for a directional compound having a hydroxyl group, and exhibits antioxidative ability by hydrogen donation through a hydroxyl group and resonance stabilization of a phenol ring structure.

To 0.2 ml of the test sample, 0.2 ml of a mixture of a 1: 2 mixture of distilled water and a Folin-Ciocalteu's phenol reagent (Sigma Chemical Co., St. Louis, Mo., USA) was added and left at room temperature for 3 minutes After 3 ml of 10% sodium carbonate (Na ₂ CO ₃ ) (Samchun Pure Chemical Co., Ltd., Pyeongtaek, Korea) was added, the reaction was allowed to proceed for 1 hour in a dark room and absorbance was measured at 765 nm.

The absorbance was applied to the calibration equation of a standard curve obtained using gallic acid, and the total polyphenol content in the sample was determined from this calibration curve.

As a result, the content of total phenolic compounds in the test sample was 425.95 ± 28.91 mg / 100 g GAE.

< Experimental Example  3>

Flavonoid compound content

The total flavonoid compound content of the antioxidant and anti-obesity compositions of the present invention was determined as follows.

Flavonoid is a component belonging to polyphenol and refers to a phenol compound which shows yellow or light yellow color. It is widely distributed in vegetables such as leaves, flowers, fruits, stems and roots of vegetables and antioxidant, anti-cancer, Have been reported to have various physiological activities.

Experimental samples 1.25㎖ 5% sodium nitrite _{(sodium nitrite, NaNO 2) (} Samchun Pure Chemical Co., Ltd.) into a 75㎕ 10% above and allowed to stand for 5 minutes to aluminum chloride hexahydrate _{(Aluminium Chloride Hexahydrate, AlCl 3 ·} 6H ₂ O) (Junsei Chemical Co., Ltd., Nihonbashi-Honcho, Japan) was added and left for 6 minutes. Then, 500 μl of 1 M sodium hydroxide (NaOH) (Samchun Pure Chemical Co., Ltd.) was added, and the absorbance was measured at 510 nm after 11 minutes.

The content of total flavonoid compounds was determined by applying absorbance to a calibration equation of a standard curve obtained by using quercetin as a standard substance.

As a result, the content of total flavonoid compounds in the test sample was 305.19 ± 37.72 mg / 100 g CE.

These flavonoid compounds are polymethoxy flavone, which is a flavonoid compound derived from black ginger, and is composed of 5,7-dimethoxy flavone, 5,7,4'-trimethoxy flavone, 5,7,3 ', 4'-tetramethoxy Hydroxy-7,7-dimethoxy flavone, 5-hydroxy-7-methoxy flavone, 5-hydroxy-7,4'-dimethoxy flavone, '-Trimethoxy flavone, 5-hydroxy-3,7,3', 4'-tetramethoxy flavone, 3,5,7-trimethoxy flavone, 3,5,7,4'-tetramethoxy Flavone, 3,5,7,3 ', 4'-pentamethoxy flavone, and the like.

< Experimental Example  4>

DPPH Radical  Scavenging activity

The DPPH radical scavenging activity of the antioxidant and anti-obesity compositions of the present invention was measured as follows.

DPPH radical scavenging activity is used as a measure to inhibit the oxidation of lipids by donating electrons to free radicals from antioxidants and is used as a measure for inhibiting free radical aging in the human body.

DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was determined by adding 0.1 ml of 0.2 mM DPPH (Sigma Chemical Co.) solution to 0.1 ml of the test sample, vortexing the mixture, reacting in a dark room for 30 minutes, Absorbance was measured. The absorbance of the sample without addition of distilled water as a sample diluting solvent was measured in the same manner.

The DPPH radical scavenging activity was expressed as a percentage in accordance with the following formula (1), and then the IC ₅₀ (50% inhibitory concentration), which is a sample concentration exhibiting a scavenging ability of 50%, was determined.

The DPPH radical scavenging activity of the test sample was 64.01 ± 2.90 mg / ㎖, which is an IC ₅₀ value, indicating a relatively high antioxidative activity.

< Experimental Example  5>

ABTS  Radical scavenging activity

The DPPH radical scavenging activity of the antioxidant and anti-obesity compositions of the present invention was determined by the ABTS radical scavenging method as follows.

The radical scavenging activity of ABTS [2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] was determined by adding 140 mM of potassium persulfate (K ₂ S ₂ O ₈ ) (Samchun) After preparing the reagents, 2 ABTS diammonium salt tablets (Sigma) were added to the mixed reagents, and 7 mM ABTS aqueous solution was prepared and left in the dark room for 14 to 16 hours. After incubation, the mixture was mixed with ethanol at a ratio of 1:88, and the ABTS solution adjusted to have an absorbance value of 0.70 ± 0.02 measured at 734 nm was used as a reagent.

1 ml of ABTS solution was added to 50 μl of the test sample, mixed in a vortex mixer (Scientific Industries Inc., NY, USA) for 10 seconds, incubated for 2 minutes and 30 seconds, and absorbance of the reaction solution was measured at 734 nm Respectively.

Absorbance was measured by the same method as in the case of adding no distilled water as a sample diluting solvent without sample treatment.

The ABTS radical scavenging activity was expressed as a percentage according to the following equation (2), and then the IC ₅₀ (inhibitory concentration) value, which indicates a scavenging ability of 50%, was obtained.

The ABTS radical scavenging activity of the test sample was 4.59 ± 0.25 mg / ㎖, which is an IC ₅₀ value, indicating high antioxidative activity.

< Experimental Example  6>

Confirm Reduction Power

The reducing power of the antioxidant and anti-obesity composition of the present invention was measured as FRAP activity as follows.

FRAP (ferric reducing antioxidant power) is based on the principle that the Fe ³⁺ -TPTZ (ferric tripyridyltriazine) complex is reduced to Fe ^{2 +} -TPTZ (ferrous tripyridyltriazine) by a reducing agent at acidic pH and its dependence on the content of antioxidants Is known to be high.

The ferric reducing antioxidant power (FRAP) activity was measured by mixing the previously prepared acetate buffer, TPTZ solution and ferric chloride solution at a ratio of 10: 1: 1 (v / v / v) And incubated at 37 DEG C for 10 minutes to prepare an FRAP reagent.

900 μl of FRAP reagent and 90 μl of distilled water were added to 30 μl of the test sample, mixed with a vortex mixer, allowed to stand at 37 ° C for 10 minutes, and the absorbance was measured at 593 nm.

The FRAP value was obtained by applying the absorbance to the calibration equation of a standard curve obtained by using iron sulfate hexahydrate (FeSO ₄ .6H ₂ O) as a standard material. The ferrous sulfate contained in 1 g of the sample MM hexahydrate.

The FRAP activity of the test sample was 60.14 ± 2.49 mM when calculated as 1 mM of the sample.

The results are shown in Table 1 below.

pH 7.45 + 0.02 Total phenolic compound 425.95 ± 28.91 mg / 100 g GAE Total flavonoid compound 305.19 占 37.72 mg / 100g CE DPPH radical scavenging activity (IC50) 64.01 占 2.90 mg / ml ABTS radical scavenging activity (IC50) 4.59 + - 0.25 mg / ml Reducing power (FRAP value), 50 mg / ml 60.14 + - 2.49 mM / g Chromaticity L 33.44 + - 0.59
a 5.88 + - 0.11
b 18.14 + - 0.50

As shown in Table 1, the antioxidant and anti-obesity compositions of the present invention were found to have high antioxidative activity by reducing power as well as antioxidative activity by radical scavenging activity, and were exposed to active oxygen such as antioxidant, anticancer, antibiotic, diabetes, The antioxidant capacity to protect the damaged DNA and the proteins and enzymes that constitute the cell is high, and it is considered that it can play a role of lowering the risk to various diseases including cancer.

< Experimental Example  7>

Anti-obesity  effect

The anti-obesity effect of the antioxidant and anti-obesity composition of the present invention was tested as follows.

The experiment was conducted on 26 men and women aged 20 ~ 50 who live in Daejeon area and took the preparation of example 2 twice for 6 days each once for 30 days.

Only 44% of participants in the experiment had experience of eating diet and medicine, and 88.9% of the participants were eating breakfast.

For the purpose of diet, 61.1% of participants answered that they want to be healthy, and 38.9% answered that they are for appearance, and it was confirmed that they think diet related to health.

In addition, 66.7% of the participants wanted exercise, diet, and medication in order to succeed in the diet, and they needed drugs or diet foods in addition to exercise and diet.

As a result of the preference test for the preparation of Example 2, it was found that the composition and taste of the preparation were generally satisfactory in addition to the inconvenience of consuming a lot of water at the time of ingestion.

Body weight, body mass index (BMI), body fat, body fat percentage, and abdominal fat percentage were measured using an inborn body composition measuring device before and after ingestion, and the results are shown in Table 2 below.

Before ingestion (B)
(n = 26) After ingestion (A)
(n = 26) A-B (% of B) Weight (kg) 70.5 ± 8.71 67.6 ± 8.62 -2.92 (4.14%) ^* Body Mass Index (BMI) 27.17 + - 2.86 26.05 + 2.81 -1.13 (4.16%) ^* Body fat (kg) 25.40 ± 6.26 23.93 ± 6.33 -1.47 (5.79%) ^* Body fat percentage (%) 35.77 ± 6.16 35.14 + - 6.63 -0.63 (1.76%) ^* Abdominal fat percentage (%) 0.92 + 0.07 0.89 ± 0.06 -0.03 (3.26%) ^* Values are mean ± standard deviation.
NS means no significant difference in paired t-test results.
* Indicates a significant difference at the p <0.05 level in paired t-test results.
WHR: Waist Hip Ratio
BMI: Body Mass Index = body weight (kg) / {height (m ² )}

As shown in the results of Table 2, weight, body mass index, body fat, body fat percentage and abdominal fat ratio were significantly decreased (p <0.05) after ingestion of the preparation of the present invention, and body weight was decreased by about 2.92 kg Respectively.

Claims (8)

50 to 70 parts by weight of a tea tumbled powder and 5 to 20 parts by weight of a black ginger powder. The method according to claim 1,
55 to 65 parts by weight of a tea tank powder and 7 to 14 parts by weight of a black ginger powder. The method according to claim 1,
Wherein said tea powder powder has a dietary fiber content of 80% or more. The method according to claim 1,
Wherein the composition further comprises 0.5 to 20 parts by weight of one or more additional components selected from green tea extract powder, aloe extract powder, prune concentrated powder and probiotics. The method according to claim 1,
Wherein the composition further comprises 0.5 to 20 parts by weight of one or more additives selected from powdered crystalline glucose, amylase, vegetable protease, enzyme-treated stevia, vegetable cream powder and maltodextrin. 5. The method of claim 4,
The probiotics may be selected from the group consisting of Streptococcus thermophilus, Lactobacillus platarium, Lactobacillus paracasei, Bifidobacterium longum, Lactobacillus lambatus, Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium breve , Lactobacillus perfumantum, Bifidobacterium bifidum, Lactobacillus debruecki sspcbulgaricus. Lactobacillus reuteri. &Lt; RTI ID = 0.0 &gt; 1. &lt; / RTI &gt; 7. A granular antioxidant and anti-obesity agent comprising the antioxidant and anti-obesity composition of any one of claims 1 to 6 formulated in a granular form. (a) an active ingredient comprising 50 to 70 parts by weight of the powdery powder and 5 to 20 parts by weight of black ginger powder; (b) a green tea extract powder; Aloe extract powder; Prune-concentrated powder; 0.5 to 20 parts by weight of one or more additional components selected from probiotics and (c) one or more additives selected from powdered crystalline glucose, amylase, vegetable protease, enzyme-treated stevia, vegetable cream powder and maltodextrin 0.5 To 20 parts by weight of a raw material;
Mixing each of the homogenized raw materials;
Adding 1 to 100 parts by weight of drinking water per 100 parts by weight of the mixture to the mixture of the raw materials;
Granulating a mixture of the raw material and the drinking water; And
And drying the granules at a temperature of 60 占 폚 to a water content of 10% or less at 60 占 占 폚.

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