KR20190027111A - Bio-diagnostic kits using nanomagnetic particles and frequency mixing magnetic reader including the same - Google Patents

Abstract

The present invention relates to a bio-diagnostic kit using nanomagnetic particles.
According to an embodiment of the present invention, there is provided a bio-diagnostic kit using nanomagnetic particles, the bio-diagnostic kit comprising: a housing including a reaction part and a treatment solution receiving part; A substrate disposed on the reaction part and having a measurement object disposed on one surface thereof; A plurality of cartridges disposed in the processing solution receiving portion, the plurality of cartridges having a processing solution contained therein and being separated into the substrate and the boundary film; And a cartridge pressing part for pressing the cartridge to break at least one of the boundary films of the plurality of cartridges to wet the processing solution on the substrate.

Description

TECHNICAL FIELD [0001] The present invention relates to a bio-diagnostic kit using nano-magnetic particles and a frequency-mixing magnetic reader including the same. ≪ Desc / Clms Page number 1 >

The present invention relates to a bio-diagnostic kit using nano-magnetic particles and a frequency-mixing magnetic reader including the same. More particularly, the present invention relates to a bio-diagnostic kit using nano-magnetic particles capable of performing all processes necessary for the pretreatment of a sample in a bio-diagnostic kit, and a frequency-mixing magnetic reader including the same.

As the average life expectancy of mankind increases, the importance of analyzing trace amounts of biomaterials in industries related to human health, such as bio-industry, medicine, and chemistry, is increasing. Due to this importance, various methods for the analysis of biomaterials have been studied. Particularly, in the technical fields such as the bio industry, analysis of biomaterials is one of the important technologies that can change the technology trend in the relevant fields as the analysis technology develops.

Methods of analyzing various biomaterials have been studied. Among them, methods using nanomagnetic particles are more stable than those of conventional biosensors in terms of physical and chemical properties, and signal generation and target substance Capturing of the image can be performed at the same time.

In order to analyze biomaterials using nanomagnetic particles, a sample is taken, preprocessed for measurement, injected into a diagnostic kit, and processed such as washing, blocking, and labeling Should proceed. Such a process has to deal with hazardous reagents to the human body and requires a trained specialist to handle the equipment that must be handled to analyze the biomaterial.

Therefore, there is a need for a method for analyzing a biomaterial using nano magnetic particles more easily by solving such a problem.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems, and it is an object of the present invention to provide a bio-diagnostic kit using nano-magnetic particles capable of performing all pretreatments necessary for measurement of a sample in a bio- .

According to an aspect of the present invention, there is provided a bio-diagnostic kit using nanomagnetic particles, the bio-diagnostic kit comprising: a housing having a reaction part and a treatment solution receiving part; A substrate disposed on the reaction part and having a measurement object disposed on one surface thereof; A plurality of cartridges disposed in the processing solution receiving portion, the plurality of cartridges having a processing solution contained therein and being separated into the substrate and the boundary film; And a cartridge pressing portion for pressing the cartridge to break at least one of the boundary films of the plurality of cartridges to wet the processing solution on the substrate.

In one embodiment, the plurality of cartridges are sequentially arranged in series in one direction, and a perforation pin may be disposed between the substrate and the boundary film on the inside of the housing.

In one embodiment, the cartridge pressing portion includes: a pressing plate for pressing the cartridge; And a lever extending from the pressing plate and exposed through a lever guide formed on a side surface of the housing, wherein the lever guide includes a first path for guiding the pressing plate to press the cartridge, And a second path for restricting movement of the pressing plate in one direction to wet only the processing solution in the cartridge on the substrate.

In one embodiment, the plurality of cartridges are arranged in parallel in one direction, and a plurality of puncturing pins may be disposed between the substrate and the boundary film on the inner side of the housing to correspond to the plurality of cartridges.

In one embodiment, the cartridge pressing portion includes: a plurality of pressing plates for respectively pressing the plurality of cartridges arranged in parallel; And a plurality of levers extending from the pressing plate and exposed through a lever guide formed on a side surface of the housing.

In one embodiment, the plurality of cartridges are juxtaposed together in one direction,

Wherein the cartridge pressing portion includes: a plurality of pressing plates for respectively pressing the plurality of cartridges arranged in parallel; A lever extended from the pressing plate in one direction and exposed to the outside of the housing; And a piercing pin disposed in the direction of the boundary membrane of the cartridge from the pressing plate, wherein the cartridge pressing portion is engaged with the piercing pin so as to be in the form of a syringe, Can be arranged.

In one embodiment, it may further comprise a gel carrier disposed on the substrate.

In one embodiment, the plurality of cartridges comprises first to fourth cartridges, the first cartridge receives a first cleaning solution, the second cartridge receives a blocking solution, Labeling solution, and the fourth cartridge can receive the second cleaning solution.

In one embodiment, the labeling solution may comprise nano-magnetic particles.

In one embodiment, the gel carrier further comprises first nanomagnetic particles disposed on the substrate, wherein the labeling solution has a second nanomagnetic particle size larger than that of the first nanomagnetic particle, Particles.

In one embodiment, the housing may include any one selected from the group consisting of polyethylene, polypropylene, and polyvinyl chloride, or a combination thereof.

According to another aspect of the present invention, there is provided a frequency-mixing magnetic reader including: a measurement head having a measurement unit capable of receiving a measurement object at a center; An excitation solenoid coil disposed inside the measurement head and exciting a frequency to the measurement unit; And a detection solenoid coil disposed inside the measurement head and detecting an output signal emitted from the measurement unit, wherein a bio-diagnostic kit using nano-magnetic particles is incorporated in the measurement unit. Here, the bio-diagnostic kit using the nano-magnetic particles may include a housing formed to be long in one direction and including a reaction part and a treatment solution receiving part; A substrate disposed on the reaction part and having a measurement object disposed on one surface thereof; A plurality of cartridges disposed in the processing solution receiving portion, the plurality of cartridges having a processing solution contained therein and being separated into the substrate and the boundary film; And a cartridge pressing portion for pressing the cartridge to break at least one of the boundary films of the plurality of cartridges to wet the processing solution on the substrate.

In another embodiment, the excitation solenoid coil includes: a first excitation solenoid coil for exciting a high frequency wave to the measurement unit; And a second excitation solenoid coil disposed outside the first excitation solenoid coil and exciting a low frequency wave to the measurement unit.

A biodiagnostic kit using nanomagnetic particles according to an embodiment of the present invention includes a plurality of cartridges each accommodating a substrate and treatment solutions inside a housing, and a user presses the cartridge with a cartridge pressurizing portion, It is possible to carry out a part of the pretreatment for analysis of biomaterials more easily.

In addition, the frequency-mixing magnetic reader according to another embodiment of the present invention can quantitatively extract the presence of a biomaterial by detecting a signal with respect to the number of nanomagnetic particles, A part of the pretreatment for the biomaterial can be easily performed using the bio-diagnostic kit using the nanomagnetic particles according to the present invention, and the bio-diagnostic kit using the nanomagnetic particles can be coupled to the measurement head to perform analysis on the biomaterial.

FIG. 1 is a schematic perspective view of a bio-diagnostic kit using nanomagnetic particles according to an embodiment of the present invention.
FIG. 2 is a schematic view illustrating the exterior of a housing of a bio-diagnostic kit using nanomagnetic particles according to an embodiment.
3 schematically shows a schematic diagram of a frequency mixing magnetic reader according to another embodiment of the present invention.
Figures 4 to 6 schematically illustrate a bio-diagnostic kit using nanomagnetic particles according to various embodiments.
* The accompanying drawings illustrate examples of the present invention in order to facilitate understanding of the technical idea of the present invention, and thus the scope of the present invention is not limited thereto.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may obscure the subject matter of the present invention.

The present invention relates to a bio-diagnostic kit using nano-magnetic particles that can easily complete a pre-treatment required for analyzing a biological material in a housing. In particular, the bio-diagnostic kit using nanomagnetic particles according to an embodiment of the present invention is coupled to the measurement head of the frequency-mixing magnetic reader according to another embodiment of the present invention, so that anyone can easily analyze biomaterials. However, the bio-diagnostic kit using nanomagnetic particles according to an embodiment of the present invention may be applied to the measuring apparatus described herein, or may be used alone.

First, a bio-diagnostic kit using a nano-magnetic particle and a frequency-mix magnetic reader will be described, and then a method of analyzing a biomaterial using a bio-diagnostic kit using a nano-magnetic particle and a frequency-mixing magnetic reader will be described .

FIG. 1 is a perspective view illustrating a perspective view of a bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment. FIG. 2 is a perspective view of a bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment. (1). The bio-diagnostic kit 100 using the nano-magnetic particles shown in Figs. 1 and 2 can be referred to as a first embodiment.

Referring to FIGS. 1 and 2, the structure of the bio-diagnosis kit 100 using nanomagnetic particles according to one embodiment will be described.

The bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment of the present invention includes a housing 1 formed to be long in one direction. The housing 1 may be cylindrical, but is not limited thereto. For example, the housing 1 may be in the form of a square column or a hexagonal column, depending on the measuring apparatus used for the measurement. A space for accommodating the biomaterial to be measured is disposed inside the housing 1.

As described later, the bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment of the present invention can perform analysis on the biomaterial C using nanomagnetic particles, May be formed of a material that does not affect magnetism. For example, the housing 1 may include any one selected from the group consisting of polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), or a combination thereof.

One end of the housing 1 may be provided with a sample inlet 12 and a lid 35 may be disposed at the other end. Here, the lid 35 can be fastened to the housing 1 by screws, separately from the housing 1. [

The housing 1 may include a reaction part 10 and a treatment solution receiving part 20.

The reaction unit 10 refers to a portion where the biological substance C is located and a part of the pretreatment and measurement are performed. The reaction part (10) includes a substrate (11) arranged at one end of the sample injection port (12).

The substrate 11 is made of a non-magnetic material so as not to affect the signal to the magnetic nanoparticles. For example, the substrate 11 may be a semiconductor wafer such as a silicon wafer, a glass such as a cover glass, or a plate made of quartz. In addition, a polymethylmethacrylate (PMMA), polypropylene (PP), polystyrene (PS), and pressure sensitive adhesive (PSA). The substrate 11 may be arranged to be inclined with respect to the longitudinal direction of the housing 1. [ That is, when the substrate 11 is arranged to be inclined and a pretreatment process is performed in the housing 1, the treatment solution can be caused to flow down.

An object to be measured is disposed on the substrate 11. When the measurement target is the biochemical material C, the gel carrier G may be disposed on the substrate 11. [ The gel carrier (G) may be made of a porous material capable of permeating a reagent such as a culture solution, a specific drug, and various aqueous solutions. The gel carrier (G) may be a sol-gel, a hydrogel, an alginate gel, an organic gel or Xerogel, a gelatin or a collagen. However, the present invention is not limited thereto, and the gel carrier G may suitably use materials known to those skilled in the art depending on the type of the biocomponent (C) to be measured.

The biocompatible material C may be at least one selected from the group consisting of DNA, RNA, oligonucleotides, peptides, proteins, antigens, antibodies, viruses, hormones, pathogenic bacteria, viruses, cells, sugars and volatile organic substances. However, the present invention is not limited thereto, and the biocomponent (C) may be a cultured virus, bacteria, cell or tissue-derived extract, lysate, purified water, blood, plasma or serum.

The biomaterial C to be measured can be injected onto the substrate 11 through the sample inlet 12. The sample inlet 12 can inject the biocide C into the housing 1 through the capillary phenomenon.

The gel carrier (G) contains a reaction substance (A) to be reacted with the biomaterial (C) to be measured. The biocomponent (C) injected through the sample inlet (12) reacts with the reactant (A). The reaction substance (A) may be a biomolecule capable of binding to the biosensor (C) by an antigen-antibody reaction, DNA complementary binding, specific reactivity of an organic compound, or the like. For example, the reactant (A) may be an antigen, an antibody, DNA, RNA, PNA, LNA, hapten, aptamer, avidin, biotin, streptavidin, neturavidin, a selectin, an enzyme, a hormone, a protein, a receptor, a ligand, an amino acid, an oligonucleotide, a peptide, an oligopeptide, and the like, but is not limited thereto.

At this time, the reaction material (A) may be in a state where the first nanomagnetic particles are bonded. The method for measuring the first nano-sized magnetic particles will be described in detail.

At the end of the reaction part 10, the treatment solution accommodating part 20 is located. The treatment solution receiving portion 20 includes a plurality of cartridges 21. The cartridge 21 is divided into a substrate 11 and a boundary film 22 of the reaction part 10 and the cartridge 21 can receive the processing solution.

Although the cartridge 21 may be composed of a plurality of cartridges 21, the first to fourth cartridges 21a, 21b, 21c, and 21d will be described herein by reference.

The bio-diagnosis kit 100 using the nano-magnetic particles according to the first embodiment is configured such that the first to fourth cartridges 21a, 21b, 21c and 21d are sequentially arranged in series in the one direction, . The second to fourth boundary films 22b, 22c, and 22d may be disposed between the first to fourth cartridges 21a, 21b, 21c, and 21d, respectively. The first boundary film 22a may be disposed between the substrate 11 and the first cartridge 21a.

At one end of the treatment solution accommodating portion 20, a cartridge pressing portion 30 is disposed. That is, in the bio-diagnosis kit 100 using the nano-magnetic particles according to the first embodiment, the cartridge pressing portion 30 is disposed in contact with the fourth cartridge 21d. The cartridge pressing portion 30 includes a pressing plate 31 and a lever 32. [ The lever 32 is exposed to the outside of the housing 1 from the pressing plate 31 through the lever guide 33 disposed outside the housing 1. [

The cartridge pressing portion 30 presses the cartridge 21 according to the user's operation by the pressing plate 31. [ The following is a little more specific. However, the description of the operation of the bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment of the present invention described below is for illustrative purposes only, and the use form of the present invention is not limited thereto.

First, a biocomponent C to be measured is injected through a sample inlet 12 into a gel carrier G disposed on a substrate 11. The injected biocomponent (C) reacts with the reactive substance (A) in the gel carrier (G).

Since only this state can not be analyzed for the biomaterial (C), it is necessary to carry out an additional pretreatment process. To this end, the biomedical diagnostic kit 100 using nanomagnetic particles according to the first embodiment of the present invention includes a first cleaning solution for the first cartridge 21a, a blocking solution for the second cartridge 21b, It is possible to fill the cartridge 21c with the labeling solution and the fourth cartridge 21d with the second cleaning solution.

The first and second cleaning solutions may serve to clean the materials that may act as noise in the measurement results. A PBS buffer may be used as the first and second washing solutions, but the method is not limited thereto, and a method well known to those skilled in the art can be used.

The blocking solution may be a substance that is different from the biocompatible material (C), and may be a material that acts to remove the reactivity of the reactant (A) that has not reacted with the biochemical (C) of the reactant (A). The blocking solution may be an antigen, an antibody, DNA, RNA, PNA, LNA, hapten, aptamer, avidin, biotin, streptavidin, neturavidin, But are not limited to, one or more selected from lectins, selectin, enzymes, hormones, proteins, receptors, ligands, amino acids, oligonucleotides, peptides, oligopeptides and the like.

The labeling solution may serve to identify the biomaterial (C) reacted with the reaction material (A) in the measuring device, and may include the second nanomagnetic particles.

The first nano-magnetic particles and the second nano-magnetic particles described above may be any of magnetic and nano-sized particles. For example, the first nano-magnetic particles and the second nano-magnetic particles may include metals, magnetic materials, magnetic alloys, A polymer magnetized to an external magnetic field, or the like can be used. The particle diameters of the first and second nano-magnetic particles may be 1 nm to 200 nm, and preferably 20 nm to 100 nm. The smaller the acceptance of the first and second nano-magnetic particles, the greater the number of particles that can be bonded to a relatively narrow substrate surface, and the range of analysis can be extended.

The first to fourth cartridges 21a, 21b, 21c and 21d are sequentially arranged in series in the longitudinal direction of the housing 1, A user who intends to perform analysis on the biocompatible material C injects the biocide C into the gel carrier G and then pushes the lever 32 along the path a ₁ of the lever guide 33 . When the lever 32 moves along the path ₁ has a pressure plate 31 and presses the cartridge 21, thus the cartridge 21 is moved according to a path _1. The lever 32 is stopped by a ₂ path at the end of a ₁ path. When the cartridge 21 is moved in accordance with a _first path, a first boundary layer (22a) by the perforated pin 13 is drilled. The perforation pin 13 may be disposed between the first boundary film 22a and the gel carrier G inside the housing 1. [ When the first boundary film 22a is punctured, the first treatment solution contained in the first cartridge 21a flows out to the gallium carrier G. For example, the first treatment solution may be the first wash solution.

Next, after moving the lever along a _second path, and by moving back along the path ₃ a boring a second boundary layer (22b). When the second boundary film 22b is punctured, the second treatment solution contained in the second cartridge 21b flows out to the gel-like support G. For example, the second treatment solution may be a blocking solution.

After performing some of the pretreatment with the second treatment solution, the lever 32 is moved along the a ₄ path. Then, by moving along the lever 32 on a path _5, and drilling a third limiting membrane (22c). When the third boundary film 22c is punctured, the third treatment solution contained in the third cartridge 21c flows out to the gel-like support G. For example, the third treatment solution may be a labeling solution comprising nanomagnetic particles.

Then, the lever 32 is moved along the a ₆ path and the a ₇ path to puncture the fourth boundary film 22d. When the fourth boundary film 22d is punctured, the fourth treatment solution contained in the fourth cartridge 21d flows out to the gel-like support G. For example, the fourth treatment solution may be a second wash solution.

As such, the processing may be performed outside the measuring apparatus, or may be performed by being coupled to the measuring apparatus. In this specification, a frequency mixing magnetic reader is used as a measuring device, but is not limited thereto. For example, in a case where a pretreatment is required to measure the biomaterial (C) as described above in another measuring apparatus, the bio-diagnosis kit 100 using nanomagnetic particles according to an embodiment of the present invention is used to measure the bio- 1), the user can easily perform the pretreatment on the biocidal material C.

Here, a ₁ , a ₃ , a _5, and a ₇ paths denote paths through which the pressure plate 31 presses the cartridge 21, which may be referred to as a first path. The paths a ₂ , a ₄ and a ₆ are formed in such a manner that the pressurizing plate 31 is disposed in the longitudinal direction of the housing of the pressing plate 31 so that only the processing solution in one cartridge is immersed in the substrate 11, And it can be referred to as a second path.

Hereinafter, a frequency-mixing magnetic reader used as a measuring apparatus will be described.

3 schematically shows a schematic diagram of a frequency mixing magnetic reader according to another embodiment of the present invention.

The frequency-mixing magnetic reader according to another embodiment of the present invention is characterized in that quantitative measurement of nonlinear magnetic particles is possible based on FMMD (Freqeuncy Mixing Magnetic Detection) technique. 3, Meas. Head refers to the measuring head 130.

The measuring head 130 includes several layers of solenoid coils. A measurement section capable of receiving an object to be measured is disposed at the center of the solenoid coil, and the bio-diagnosis kit 100 using the nano-magnetic particles according to an embodiment of the present invention is coupled to the measurement section.

The solenoid coil may include excitation solenoid coils 112 and 122 for exciting a frequency to a measurement object, and a detection solenoid coil 150 for detecting an output signal emitted from the measurement unit.

The solenoid coils 112 and 122 include a first excitation solenoid coil 112 and a second excitation solenoid coil 122. The first excitation solenoid coil 112 may excite a first frequency, that is, a high frequency generated by the high frequency generation module 110 and amplified through the amplifier 111, to the measurement unit. The second excitation solenoid coil 122 may be disposed outside the first excitation solenoid coil 112, i.e., at the outermost angle of the measurement head 130. The second excitation solenoid coil 122 may excite a second frequency, that is, a low frequency generated by the low frequency generation module 120 and amplified through the amplifier 121, to the measurement unit. However, the present invention is not limited to this, and it is possible to mix signals beforehand in an electronic device such as a combiner so as to generate a magnetic field field equal to the sum of the magnetic field fields generated by the first and second excitation solenoid coils. It is also possible to excite the mixed signal from one excitation solenoid coil to the measuring unit.

The high frequency generating module 110 generates a high frequency sinusoidal signal corresponding to Y ₁ = sin (2 × π × f ₁ × t) based on the fundamental frequency. At this time, the sinusoidal wave can be represented by a sine function and correspond to a basic waveform used for displaying a spatial frequency or a sound. In addition, 2 x? X f ₁ can mean?, Which is an angular frequency with respect to the frequency f ₁ , and t denotes a period. Thereafter, the amplifier 111 can amplify the Y ₁ signal to an intensity corresponding to A ₁ . At this time, the signal obtained by amplifying the intensity of the Y ₁ signal may correspond to Y ₃ = A ₁ × sin (2 × π × f ₁ × t).

The low frequency generation module 120 generates a low frequency sinusoidal signal corresponding to Y ₂ = sin (2 × π × f ₂ × t) based on the fundamental frequency. Thereafter, the amplifier 121 can amplify the Y ₂ signal to an intensity corresponding to A ₂ . At this time, the signal obtained by amplifying the intensity of the Y ₂ signal may correspond to Y ₄ = A ₂ × sin (2 × π × f ₂ × t).

The signal Y ₅ = A ₁ × sin (2 × π × f ₁ × t) + A ₂ × sin (2 × π × f ₂ × t) amplified by the amplifiers is applied to the excitation solenoid coils 112 and 122 .

The detection solenoid coil 150 is disposed inside the solenoid coils 112 and 122. The detection solenoid coil 150 detects the output signal emitted by the measurement object after the frequency generated by the excitation solenoid coils 112 and 122 is applied to the measurement object located in the measurement unit. Referring to FIG. 3, the detection solenoid coil 150 may include a plurality of detection solenoid coils 150. For example, the detection solenoid coil 150 may include upper and lower detection solenoid coils depending on the insertion direction of the measurement object.

Non-linear magnetic particles can be quantitatively measured based on the FMMD (Freqeuncy Mixing Magnetic Detection) technique according to another embodiment of the present invention. Here, the nonlinear magnetic particle means a material or particle having nonlinear magnetic properties It can mean. That is, nonlinear magnetic particles may refer to a material or particle that is not proportional to the strength or magnitude of the magnetic field produced by the excitation solenoid coils 112 and 122. In the present invention, the nonlinear magnetic particles may be the first nano-magnetic particles contained in the gel carrier (G) or the second nano-magnetic particles included in the labeling solution.

The method of measuring the biochemical material C using the frequency-mixing magnetic reader according to another embodiment of the present invention can be largely divided into two methods.

The first method is to perform a measurement on the biocompatible material (C) using two kinds of nanomagnetic particles, i.e., first and second nanomagnetic particles.

When the first nano-magnetic particles are used, it is assumed that the gel carrier (G) contains a sufficient number of reactant (A) preliminarily bound to the first nano-magnetic particles. Thereafter, when the biocomponent C to be measured is injected, the reactant A binds to the biocompatible material C of the gel-like support G. The first state is defined as a state in which the reaction material (A) bound to the first nanomagnetic particles reacts with the biomaterial (C). Thereafter, the first washing step and the blocking step are sequentially performed, and labeling is performed on the biocompatible material (C) bound to the reactive material (A) by using the labeling solution containing the second nano-sized magnetic particles. As described above, the state in which the second nanomagnetic particles are combined with the biomaterial (C) combined with the reactive substance (A) is defined as the second state. A second cleaning step is then performed to clean the remaining second nanomagnetic particles.

At this time, the particle diameters of the first nanomagnetic particle and the second nanomagnetic particle may be different from each other. For example, the particle diameter of the first nano-magnetic particle may be smaller than the particle diameter of the second nano-magnetic particle. In this case, the signals to be measured are different for each step in the frequency mixing magnetic reader described below. Therefore, the signal of the first state and the state of the second state can be compared, and the biochemical material C can be analyzed more accurately.

The second method may not use the first nano-magnetic particles in the measurement of the biomaterial (C). In this case, it is assumed that the gel carrier (G) contains a sufficient number of reactants (A). Then, when the biocomponent (C) to be measured is injected, the reactant (A) binds to the biocomponent (C). Thereafter, the first washing step and the blocking step are sequentially performed, and labeling is performed on the biocompatible material (C) bound to the reactive material (A) by using the labeling solution containing the second nano-sized magnetic particles. A second cleaning step is then performed to clean the remaining second nanomagnetic particles. In this case, it is possible to perform measurement on the biocompatible material C by analyzing a signal according to the second nano-magnetic particles in a frequency-mixing magnetic reader described below.

On the other hand, the method of performing the necessary preprocessing inside the housing 1 is not limited to the first embodiment, but may be carried out in other forms as will be described later.

4 and 5 are a perspective view and a perspective view of the housing of the bio-diagnosis kit 100a using the nano-magnetic particles according to the second embodiment of the present invention, respectively.

In the bio-diagnosis kit 100a using the nano-magnetic particles according to the second embodiment, description of the same configuration as the bio-diagnosis kit using the nano-magnetic particles according to the first embodiment will be omitted.

A plurality of cartridges 21 are arranged in parallel in the longitudinal direction of the housing 1 in the biomedical diagnostic kit 100a using the nano magnetic particles according to the second embodiment. The cartridge 21 may be provided with the first to fourth cartridges 21a, 21b, 21c and 21d in the counterclockwise direction, but is not limited to the order in which they are arranged.

One end of each of the first to fourth cartridges 21a to 21d is connected to the gel carrier G of the reaction part 10 by first to fourth boundary films 22a, 22b, 22c, Respectively.

The first to fourth pressing plates 31a, 31b, 31c, and 31d are disposed at the other ends of the first to fourth cartridges 21a, 21b, 21c, and 21d, respectively. That is, unlike the bio-diagnosis kit 100 using the nano-magnetic particles according to the first embodiment, the bio-diagnosis kit 100a using the nano-magnetic particles according to the second embodiment is different from the bio- Only the cartridge 21 is pressed.

Therefore, in order to perforate the cartridge 21 pressed by one perforation pin 13 when only one cartridge 21 is pressed with one pressing plate 31, The bio-diagnosis kit 100a using magnetic particles has a plurality of perforation pins 13, that is, first through fourth perforation pins 13a, 13b, and 13c at positions corresponding to the first through fourth cartridges 21a, 21b, 21c, 13b, 13c and 13d are arranged.

Looking at the operation of the Bio-Test Kit (100a) using the nano-magnetic particle according to a second embodiment of the present invention in detail, by moving the first lever (32a) in a _first direction along the first lever guide (33a), the The first cartridge 21a is pressed by the first pressing plate 31a so that the first boundary film 22a of the first cartridge 21a is punctured by the first puncturing pin 13a, So that the first treatment solution in the first carrier 21a flows into the gel carrier G.

Next, the second lever (32b) to the second along the lever guide (33b) moving in a _second direction, the second to the pressure plate (31b) and presses the second cartridge (21b), this second cartridge (21b along Is punctured by the second perforation pin 13b so that the second treatment solution in the second cartridge 21b flows into the gel carrier G. [

Then, the first being to urge the third cartridge (21c) a third lever (32c) to the third lever guide (33c) by moving the along a _third direction, the third press plate (31c), In the third cartridge according to ( The third boundary film 22c of the third cartridge 21c is punctured by the third perforation pin 13c and the third treatment solution in the third cartridge 21c flows to the gel carrier G. [

Finally, the second and the fourth pressing the lever (32d) of the fourth lever guide (33d) a fourth cartridge (21d) by moving a _fourth direction, the fourth pressing plate (31d) along, whereby the fourth cartridges ( The fourth boundary film 22d of the first cartridge 21d is punctured by the fourth perforation pin 13d and the fourth treatment solution in the fourth cartridge 21d flows into the gel carrier G. [

The bio diagnostic kit 100a using the nano magnetic particles according to the second embodiment of the present invention is different from the bio diagnostic kit 100 using the nano magnetic particles according to the first embodiment, , 21b, 21c, and 21d can be selected.

FIG. 6 is a schematic perspective view of a bio-diagnosis kit 100b using nanomagnetic particles according to a third embodiment of the present invention.

Description of the same configuration as that of the bio-diagnosis kit 100 using the nano-magnetic particles according to the first embodiment in the bio-diagnosis kit 100b using the nano-magnetic particles according to the third embodiment will be omitted.

The plurality of cartridges 21 are arranged in parallel in the longitudinal direction of the housing 1 in the biomedical diagnostic kit 100b using the nano magnetic particles according to the third embodiment. The cartridge 21 may be provided with the first to fourth cartridges 21a, 21b, 21c and 21d in the counterclockwise direction, but is not limited to the order in which they are arranged.

One end of each of the first to fourth cartridges 21a to 21d is connected to the gel carrier G of the reaction part 10 by first to fourth boundary films 22a, 22b, 22c, Respectively.

The bio-diagnosis kit 100b using nanomagnetic particles according to the third embodiment of the present invention is configured in the form of a syringe by combining the structures of the cartridge 21 and the cartridge pressurizing portion 30. [ 6, the cartridge pressing portion 30 includes first to fourth pressing plates 31a, 31b, 31c, and 31d for pressing the first to fourth cartridges 21a, 21b, 21c, and 21d, 32b, 32c, 32d which extend to the rear of the first to fourth pressing plates 31a, 31b, 31c, 31d and are exposed to the outside of the housing 1. [ A plurality of perforation fins 13a, 13b, 13c, and 13d are disposed in front of the first to fourth pressing plates 31a, 31b, 31c, and 31d, that is, in the direction of the boundary film 22 from the pressing plate 31 . The difference from the ordinary syringe is that the perforation pin 13 is disposed inside the cartridge 21 to puncture the boundary film 22 inside the cartridge 21 when pressurized.

The cartridge 21 of the bio-diagnosis kit 100b using the nano magnetic particles according to the third embodiment may be in a fixed state unlike the first embodiment or the second embodiment. That is, even if the user presses the cartridge 21 using the lever 32, the cartridge 21 itself does not move, and pressure is applied to the processing solution contained in the cartridge 21. That is, when the user presses the cartridge 21 using the lever 32, the perforation pin 13 punctures the boundary film 22 of the cartridge 21 and causes the cartridge 21 to be received The treatment solution that has been supplied to the gel carrier G flows out.

Looking at the operation of the Bio-Test Kit (100b) using the nano-magnetic particle according to a third embodiment of the present invention in detail, by pressing a first lever (32a) in a _first direction, the first pressure plate (31a) of the first The cartridge 21a is pressed so that the first boundary film 22a of the first cartridge 21a is punctured by the first puncturing pin 13a and the first processing solution in the first cartridge 21a And flows to the gel carrier (G).

And then, pressing the second lever (32b) in a ₁ direction, the second pressing plate (31b) and a second boundary layer (22b) of the first and presses the second cartridge (21b), this second cartridge (21b) in accordance with Is punctured by the second perforation pin 13b and the second treatment solution in the second cartridge 21b flows to the gel carrier G. [

Then, by pressing the third lever (32c) in a ₁ direction, a third pressure plate (31c) are first and presses the third cartridge (21c), Accordingly, the third limiting membrane (22c of the third cartridge (21c) Is punctured by the third perforation pin 13c and the third treatment solution in the third cartridge 21c flows into the gel carrier G. [

Finally, the fourth pressing the lever (32d) in a ₁ direction, and the fourth pressing plate (31d) a fourth limiting membrane (22d of the first and presses the fourth cartridge (21d), this fourth cartridge (21d) in accordance with Is punctured by the fourth perforation pin 13d and the fourth treatment solution in the fourth cartridge 21d flows to the gel carrier G. [

The bio-diagnosis kit 100b using the nano-magnetic particles according to the third embodiment of the present invention is different from the bio-diagnosis kit 100 using the nano-magnetic particles according to the first embodiment, , 21b, 21c, and 21d can be selected. The biomedical diagnostic kit 100b using the nano magnetic particles according to the third embodiment of the present invention has a structure in which the cartridge 21 and the cartridge pressurizing portion 30 are formed in the form of a syringe, The amount of the solution can be precisely controlled.

The scope of protection of the present invention is not limited to the description and the expression of the embodiments explicitly described in the foregoing. It is again to be understood that the present invention is not limited by the modifications or substitutions that are obvious to those skilled in the art.

100: Bio-diagnosis kit using nanomagnetic particles
10: Reactor 11: Substrate
12: Sample inlet 13: Perforated pin
20: treatment solution receiving portion 21: cartridge
22:
30: cartridge pressing portion 31: pressure plate
32: Lever 33: Lever guide
35: Cover
C: Biological substance A: Reactive substance
G: Gel carrier

Claims (13)

A housing which is elongated in one direction and includes a reaction part and a treatment solution accommodating part;
A substrate disposed on the reaction part and having a measurement object disposed on one surface thereof;
A plurality of cartridges disposed in the processing solution receiving portion, the plurality of cartridges having a processing solution contained therein and being separated into the substrate and the boundary film; And
And a cartridge pressing portion for pressing the cartridge to urge the boundary film of at least one of the plurality of cartridges to wet the processing solution on the substrate. The method according to claim 1,
Wherein the plurality of cartridges are sequentially arranged in series in one direction,
Wherein a perforation pin is disposed between the substrate and the boundary film inside the housing. 3. The method of claim 2,
The cartridge pressing portion includes:
A pressing plate for pressing the cartridge; And
And a lever extending from the pressing plate and exposed through a lever guide formed on a side surface of the housing,
Wherein the lever guide has a first path for guiding the pressurizing plate to press the cartridge and a second path for restricting movement of the pressurizing plate in one direction so that the pressurizing plate wets only the processing solution in the one cartridge with the substrate, Wherein the nanomagnetic particles are dispersed in the nanomagnetic particles. The method according to claim 1,
The plurality of cartridges are arranged together in parallel in one direction,
And a plurality of puncturing fins are disposed between the substrate and the boundary film on the inner side of the housing to correspond to the plurality of cartridges. 5. The method of claim 4,
The cartridge pressing portion includes:
A plurality of pressing plates for respectively pressing the plurality of cartridges arranged in parallel; And
And a plurality of levers extending from the pressing plate and exposed through a lever guide formed on a side surface of the housing. The method according to claim 1,
The plurality of cartridges are arranged together in parallel in one direction,
The cartridge pressing portion includes:
A plurality of pressing plates for respectively pressing the plurality of cartridges arranged in parallel;
A lever extended from the pressing plate in one direction and exposed to the outside of the housing; And
And a piercing pin disposed in the direction of the boundary film of the cartridge from the pressing plate,
Wherein the cartridge is configured in the form of a syringe by coupling the cartridge pressing portion,
And a puncturing pin is disposed on the inside of the cartridge on one side of the pressing plate. The method according to claim 1,
And a gel carrier disposed on the substrate. The method according to claim 1,
Wherein the plurality of cartridges include first to fourth cartridges,
The first cartridge receives the first cleaning solution,
The second cartridge receives the blocking solution,
The third cartridge receives the labeling solution,
And the fourth cartridge includes nano-magnetic particles for accommodating a second cleaning solution. 8. The method of claim 7,
Wherein the labeling solution is nanomagnetic particles comprising nanomagnetic particles. 8. The method of claim 7,
Further comprising a gel carrier disposed on the substrate,
Wherein the gel carrier comprises first nanomagnetic particles,
Wherein the labeling solution comprises nanomagnetic particles comprising second nanomagnetic particles having a larger particle size than the first nanomagnetic particles. The method according to claim 1,
Wherein the housing comprises any one selected from the group consisting of polyethylene, polypropylene, and polyvinyl chloride, or a combination thereof. A measurement head having a measurement section capable of receiving a measurement object at a central portion thereof;
An excitation solenoid coil disposed inside the measurement head and exciting a frequency to the measurement unit; And
And a detection solenoid coil disposed inside the measurement head and detecting an output signal emitted from the measurement unit,
The frequency-mixing magnetic reader according to any one of claims 1 to 11, wherein the bio-diagnostic kit using the nanoparticles is coupled to the measurement unit. 13. The method of claim 12,
The excitation solenoid coil includes:
A first excitation solenoid coil for exciting a high frequency wave to the measurement unit; And
And a second excitation solenoid coil disposed outside the first excitation solenoid coil and exciting a low frequency wave to the measurement unit.

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