KR20180007834A - Composition for cell regeneration comprising extract of ginseng floral axis - Google Patents

Abstract

In the specification, a composition for cell regeneration or anti-inflammation comprising an extract of a ginseng floral axis as an active ingredient is provided, wherein the extract is prepared by using water, C_1-C_5 alcohol or a C_1 alcohol solution, and the composition may be used as health food, cosmetic materials or pharmaceutical compositions, and has excellent skin regeneration or wound healing effect, and an anti-inflammatory effect since the composition promotes cell regeneration.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for cell regeneration,

The present invention relates to a composition for cell regeneration or antiinflammation comprising an extract of Ganoderma lucidum as an active ingredient.

There have been many studies on extracts of ginseng root, ginseng fruit or ginseng flower. For example, there have been studies on cosmetic composition for whitening using ginseng flower, and research on skin diseases.

However, there was no study on the extracts of ginseng flower which were discarded after ginseng harvest, and there was no study of cell regeneration on the ginseng flower extract.

Korea Patent No. 10-0525008 Korean Patent No. 10-1366495 Korean Patent No. 10-1082277

In view of the above problems, the inventors of the present invention conducted research on ginseng phalaenopsis extract, and found that the ginseng phalaenopsis extract had cell regeneration and anti-inflammatory effects, thus leading to the present invention.

One aspect of the present invention is to provide a composition for cell regeneration comprising an extract of ginseng flower as an active ingredient.

One aspect of the present invention is to provide an anti-inflammatory composition comprising an extract of ginseng flower as an active ingredient.

In one aspect of the present invention, a composition for cell regeneration or anti-inflammation is to provide a health food, a cosmetic composition or a pharmaceutical composition.

One aspect of the present invention provides a composition for cell regeneration comprising an extract of ginseng flower as an active ingredient.

An aspect of the present invention provides an anti-inflammatory composition comprising an extract of Ganoderma lucidum as an active ingredient.

In one aspect of the present invention, the extract is an extract which is extracted with water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol aqueous solution.

In one aspect of the present invention, the concentration of the aqueous C ₁ -C ₅ alcohol solution is from 40% to 90% (v / v).

In one aspect of the present invention, the C ₁ -C ₅ alcohol is at least one selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. do.

In one aspect of the present invention, the extract is an extract which is extracted with an ethanol aqueous solution having a concentration of 60 to 80% (v / v).

In one aspect of the present invention, the ginseng peduncle is obtained by removing ginseng fruit.

In one aspect of the present invention, the composition for cell regeneration is a composition for wound healing.

In one aspect of the present invention, the composition for cell regeneration promotes skin regeneration.

In one aspect of the invention, the composition provides a health food or cosmetic composition.

In one aspect of the invention, the composition provides a pharmaceutical composition.

In one aspect of the present invention, the composition provides a composition formulated in the form of a powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, and external preparation.

The composition according to one aspect of the present invention has cell regeneration effect.

The composition according to one aspect of the present invention has anti-inflammatory effect.

The composition according to one aspect of the present invention has an effect of promoting skin regeneration.

The composition according to one aspect of the present invention has an effect of promoting wound healing.

One aspect of the present invention is to provide a health food composition having cell regeneration effect.

One aspect of the present invention is to provide a health food composition having skin regeneration effect.

One aspect of the present invention is to provide a health food composition having wound healing effect.

One aspect of the present invention is to provide a health food composition having an anti-inflammatory effect.

One aspect of the present invention is to provide a cosmetic composition having cell regeneration effect.

One aspect of the present invention is to provide a cosmetic composition having skin regeneration effect.

One aspect of the present invention is to provide a cosmetic composition having wound healing effect.

One aspect of the present invention is to provide a cosmetic composition having an anti-inflammatory effect.

One aspect of the present invention is to provide a pharmaceutical composition having cell regeneration effect.

One aspect of the present invention is to provide a pharmaceutical composition having skin regeneration effect.

One aspect of the present invention is to provide a pharmaceutical composition having wound healing effect.

One aspect of the present invention is to provide a pharmaceutical composition having an anti-inflammatory effect.

The composition according to one aspect of the present invention is effective in discovering the value of a discarded portion due to the recycling of the discarded ginseng leaf during the processing of ginseng.

FIGS. 1 and 2 are experimental results comparing the cell migration effect of Experimental Example 2 of the present invention with that of a ginseng flower, red ginseng, ginseng flower, ginseng fruit, a positive control and a negative control.
FIG. 3 is a graph showing the results of experiments according to Experimental Example 3 of the present invention, wherein the y-axis represents the% value obtained by converting the control group into 100 units.

Generally, ginseng refers to 'ginseng root' which is the root part of ginseng, and 'ginseng root', which is used as a medicinal material or cooked with food, is the root part. Ginseng (Panax ginseng CA Meyer) is a plant belonging to the genus Omega and Ginseng. It has been used in Korea, China and Japan for the purpose of preventing diseases and prolonging the life span. The ginseng efficacy and effects known so far are anti- , Anticancer activity, immune function regulating action, antidiabetic action, hyperfunction of liver function, improvement of cardiovascular disorder, blood pressure regulating action, improvement of menopausal disorder, anti-stress and anti-fatigue action, antioxidant activity and anti-aging effect.

The ginseng peduncle is not usually used as a medicinal herb but it is hard to find a history of ingesting it in the form of food. The ginseng peduncle is planted and seeds are planted around May to June when it is 4 years old. It is removed with ginseng flower around June before full bloom for smooth growth of ginseng root.

Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. Especially, ginsenoside (root), ginseng leaf and ginseng berries have not only ginsenoside content but also composition . This difference in composition makes a difference in the presence or absence of various effects and causes different site-specific efficacy characteristics. For example, ginseng flower extracts have been found to have an excellent efficacy of about 23 times more effective in preventing and treating diabetes than ginseng root extracts.

In this specification, it was found that the ginseng phloem extract has cell regeneration and anti-inflammatory effect by evaluating the cell regeneration efficiency and anti-inflammatory effect of the ginseng phloem part which has been abandoned and not used at present.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a composition for cell regeneration comprising an extract of ginseng flower as an active ingredient.

An aspect of the present invention provides an anti-inflammatory composition comprising an extract of Ganoderma lucidum as an active ingredient.

In this specification, "ginseng flower bed" is a shaft part of a ginseng flower, which is a peduncle and a flower stalk.

As used herein, the term "extract" refers to a substance obtained by extracting a component contained therein from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept including both extracts of components dissolved in a solvent from natural products using water or an organic solvent, and extracts obtained by extracting only specific components of natural products. In one embodiment of the present invention, the organic solvent is not particularly limited, and may be a C1 to C5 lower alcohol such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol, Polyhydric alcohols such as ethylene glycol, propylene glycol and 1,3-butylene glycol; hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane and chloroform; And nonpolar organic solvents such as benzene, hexane, chloroform, methylene chloride, dimethyl ether, ethyl acetate and the like.

In one embodiment, the extract may be water, especially an extract that is extracted using hot water. In another embodiment, the extract may be an extract that is extracted using a C ₁ -C ₅ alcohol. In another embodiment, the extract may be, but is not limited to, extracts extracted with ethanol.

In one aspect of the present invention, the extract is an extract which is extracted with water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol aqueous solution.

In one aspect of the present invention, the concentration of the aqueous C ₁ -C ₅ alcohol solution is from 40% to 90% (v / v). In one embodiment, the concentration of the aqueous C ₁ -C ₅ alcohol solution is greater than 40%, greater than 45%, greater than 50%, greater than 55%, greater than 60%, greater than 65%, greater than 66%, greater than 67%, greater than 68% , 69% or more, 70% or more, 71% or more, 72% or more, 73% or more, 74% or more, 75% or more or 80% or more. The concentration of the aqueous C ₁ -C ₅ alcohol solution may be 90% or less, 85% or less, 80% or less, 75% or less, 74% or less, 73% or less, 72% or less, 71% or less, Or less, 68% or less, 67% or less, 65% or less, or 60% or less. When the concentration of the aqueous C ₁ -C ₅ alcohol solution is within the above range, the cell regeneration efficiency or anti-inflammatory activity is excellent.

In one aspect of the present invention, the C ₁ -C ₅ alcohol is at least one selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. do.

In one aspect of the present invention, the extract is an extract which is extracted with an ethanol aqueous solution having a concentration of 60 to 80% (v / v).

In one aspect of the present invention, the ginseng extract may be contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. In one embodiment, the ginseng extract is present in an amount of at least 0.001%, at least 0.01%, at least 0.1%, at least 1%, at least 1.1%, at least 1.5%, at least 2% , 3 wt% or more, 5 wt% or more, 10 wt% or more, 20 wt% or more, or 30 wt% or more. The ginseng flower extract may be used in an amount of 90 wt% or less, 85 wt% or less, 80 wt% or less, 70 wt% or less, 50 wt% or less, 40 wt% or less, 30 wt% or less, 20 wt% Up to 10 weight percent, up to 5 weight percent, up to 4 weight percent, up to 3 weight percent, up to 2 weight percent, up to 1 weight percent, up to 0.1 weight percent or up to 0.05 weight percent.

In one aspect of the present invention, the ginseng peduncle is obtained by removing ginseng fruit.

In one aspect of the present invention, the composition for cell regeneration is a composition for wound healing. Specifically, the composition for cell regeneration may be to heal wounds on the skin.

In one aspect of the present invention, the composition for cell regeneration promotes skin regeneration. Specifically, the skin regeneration may promote regeneration of skin cells.

In one aspect of the invention, the composition may be to regenerate the wrinkles of the skin.

In one aspect of the invention, the composition may be to regenerate aged skin.

In one aspect of the invention, the composition may be to regenerate the scar.

In one aspect of the invention, the composition may be to enhance the volume feel of the skin.

In one aspect of the present invention, the composition may be to smooth the uneven surface of the skin.

In one aspect of the invention, the composition provides a health food or cosmetic composition.

In one aspect of the present invention, the composition provides a composition formulated in the form of a powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, and external preparation.

In one aspect of the invention, the composition may be a cosmetic composition. The cosmetic composition of the present invention may be prepared in any formulation conventionally produced in the art and may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

When the ginseng flower extract is used as a cosmetic composition, the dose determination is within the level of those skilled in the art. The daily dose of the composition depends on various factors such as the age, health condition, and complication of the subject to be administered, , It is generally possible to administer the ginseng flower extract at a dose of 1 to 500 mg / kg, preferably 30 to 200 mg / kg, once or twice a day, Is not to limit the scope of

In one aspect of the present invention, the composition may be a food composition, for example, various foods, beverages, gums, tea, vitamin complexes, health supplements, etc., and may be in the form of powder, granule, tablet, capsule or beverage . The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect may occur when they are applied simultaneously with other ingredients.

The amount of the active ingredient contained in the food or beverage may generally be from 0.1 to 60% by weight of the whole food when it is a health food composition, and 0.002 to 50 g based on 100 ml and 0.02 To 10 g.

When the ginseng phloem extract is used as a food composition, the dosage determination is within the level of those skilled in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / Day to 500 mg / kg / day, but it is not limited thereto, and may vary depending on various factors such as the age, health condition, and complication of the subject to be administered.

In one embodiment, the composition may contain other ingredients, etc., which may give rise to synergistic effects on the main effect, to the extent that the main effect of the present invention is not impaired. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01-5 wt%, more specifically 0.01-3 wt%, based on the total weight of the composition.

In one aspect of the invention, the composition may be a pharmaceutical composition. In the manufacture of pharmaceutical formulations, it is preferred that the active ingredient is mixed with or diluted with the carrier, or enclosed within a carrier in the form of a container. When the carrier is used as a diluent, it may be a solid, semi-solid or liquid substance acting as a carrier, excipient or medium for the active ingredient. Thus, the formulations may be in the form of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injections, sterile powders and the like. Examples of suitable carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, Hydroxybenzoate, talc, magnesium stearate, and mineral oil. The formulations may additionally include fillers, anti-coagulants, lubricants, wetting agents, perfumes, emulsifiers, preservatives, and the like. The pharmaceutical compositions of the present invention may be formulated using methods well known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal. The pharmaceutical composition of the present invention may be administered through various routes including oral, transdermal, subcutaneous, intravenous, intraperitoneal, muscle, topical application, patch and iontophoresis, and local application and oral administration are preferred can do. In the case of humans, the usual daily dose of the active compound may range from 0.1 to 100 mg / kg body weight, preferably from 5 to 70 mg / kg body weight, and may be administered in single or divided doses. It should be understood, however, that the actual dosage of the active ingredient should be determined in light of various relevant factors such as the disease to be treated, the route of administration, the age, sex and weight of the patient, and the severity of the disease, Are not intended to limit the scope of the invention.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example  One : Ginseng flower  Preparation of extract

In case of ginseng phloem extract, ginseng phyllodes were collected and the fruit part was separated and removed. Then, the extract was dried with sunshine or hot air to prepare ginseng flower. After that, 70% ethanol was added to 1 kg of dried ginseng flower, and the mixture was refluxed, filtered and concentrated under reduced pressure at 40-45.

The ginseng extract obtained from the above process was used in the following experimental examples.

Comparative Example  1 to 4

The red ginseng prepared by pulverizing and drying the ginseng harvested from Geumsan as Comparative Example 1 was extracted and used by the same extraction method as in Example 1.

In Comparative Example 2, the same extraction method as in Example 1 was used to extract ginseng flower instead of ginseng flower.

In Comparative Example 3, the same extraction method as in Example 1 was used to extract ginseng roots instead of ginseng roots.

In Comparative Example 4, the extract was extracted by the same extraction method as in Example 1 except that the raw material was ginseng fruit instead of ginseng flower.

The above Comparative Examples 1 to 4 were used in the following experimental examples.

Experimental Example  1: Ginsenoside analysis of extracts from ginseng flower

The ginseng flower extract of Example 1, the ginseng root extracts of Comparative Examples 3 to 4, and ginsenosides of ginseng fruit extract were analyzed according to the guidelines of Healthy Foods.

The ginsenoside analysis results of the ginseng flower buds, ginseng roots and ginseng fruits according to the above experiment are shown in Table 1 below.

sample PPT PPD PPT / PPD Rg1 Re Rb1 Rc Rb2 Rd Ginseng flower
(Example 1) 1.38 6.00 0.61 1.18 0.5 0.93 2.29 Ginseng roots
(Comparative Example 3) 0.5 0.4 0.9 One 0.3 0.3 0.36 Ginseng berries
(Comparative Example 4) 1.9 10 0.8 2.1 2 1.8 1.78

As shown in Table 1, the ginseng phlox extract of Example 1 of the present invention was found to have differences in ginsenoside root and ginseng fruit extract and ginsenoside components. Therefore, it was confirmed that the ginseng root and ginseng fruit extract and the ginseng flower extract of the present invention were different in the content of ginsenosides.

Experimental Example  2: Cell migration assay experiment

Cell migration assay was performed to examine cell regeneration effect and wound healing effect of ginseng extract of Example 1 (70% ethanol extract).

The experiment was carried out according to the following method.

HaCaT, a keratinocyte purchased from ATCC, was cultured in a dish of 95% or more and scratched with a yellow tip to form a wound. 2% FBS (fetal bovine serum, Welin) was applied to the wounded dish, and then the sample as shown in Table 2 was treated, and the degree of recovery over time was measured and quantified. The moving distance was measured by using a microscope, and the same area was measured and compared at the 100-fold ratio using the Toupview program.

As a positive control, a medium containing 10% FBS without a sample was used as a positive control. As a negative control, FBS and a medium not treated with the sample were used as a negative control. As a normal control, a medium containing 2% FBS As a general control group.

sample The concentration contained in the medium Comparative Example 1 (red ginseng) 50 ppm Example 1 (Ginseng Vinegar) 50 ppm Comparative Example 2 (Ginseng flower) 50 ppm Comparative Example 4 (Ginseng fruit) 50 ppm

The experimental results are shown in Figs. 1 and 2. Fig.

Experimental Results As shown in FIGS. 1 and 2, the movement distance of the keratinocytes after wounding was measured. As a result, the movement distance of the ginseng extract of Example 1 was superior to that of Comparative Examples 1, 2 and 4, The ginseng flower bud of Example 1 tended to recover as much as the positive control group. Therefore, the ginseng flower extract of Example 1 was excellent in cell regeneration effect. In addition, it was confirmed that the ginseng flower extract of Example 1 was effective for wound healing or skin regeneration.

Experimental Example  3: CCK8  assay (evaluation of viability after inflammation)

The CCK8 assay was performed to evaluate the viability of the ginseng extract of Example 1 (70% ethanol extract) after the inflammation treatment.

The experiment was carried out according to the following method.

The viability of the cell line HaCaT, which is a keratinocyte, was evaluated by treatment with 500 mg of CoCl ₂ as an inflammatory inducer. In a 96-well microplate, seed the cells with 5 x 104 cells / well of HaCaT cel in 100 μl of culture medium (DMEM 10% FBS, P / S 1%). After 16 hours, the efficacious substance (Table 3) and the inflammation inducing factor CoCl 2 (500 μM) are treated simultaneously and cultured in a CO 2 incubator for 24 hours. Remove the cell culture medium, wash twice with PBS, add 10 μl of Cell Counting Kit-8 and 100 μl of culture solution, and mix well. After incubation for 1 hour in a CO2 incubator, the absorbance at 450 nm is measured using a microplate reader. As a general control group, HaCaT cell line without CoCl2 treatment was used.

Efficacy substance Treatment concentration Comparative Example 1 (red ginseng) 100ppm Example 1 (Ginseng Vinegar) 100ppm Comparative Example 2 (Ginseng flower) 100ppm Comparative Example 4 (Ginseng fruit) 100ppm

As shown in FIG. 3, the survival rate of the CoCl ₂ -treated group was reduced by about 40%, but the survival rate of the Ganoderma lucidum extract-treated group (Example 1) was about 91%. In addition, the ginseng flower extract of Example 1 showed an excellent survival rate as compared with Comparative Examples 1, 2 and 4. Thus, it was confirmed that the extract of Ganoderma lucidum in Example 1 was excellent in anti-inflammatory effect.

Hereinafter, a formulation example of the composition according to the present invention will be described, but this is not intended to limit the invention, but merely to illustrate it.

[Formulation Example 1] Production of pills

30% by weight of Example 1, 30% by weight of corn starch, 20% by weight of glycerin and 20% by weight of sorbitol powder were mixed and a ring was prepared using a ventilator. The final weight of the contents was 3.5 g.

[Formulation Example 2] Preparation of tablet

A sugar ester was prepared by mixing 30 wt% of the extract of Example 1, 20.5 wt% of lactose, 20 wt% of dextrin, 20 wt% of maltitol powder and 7 wt% of xylitol powder, using a fluid bed drier, 2.5% by weight was added and the tablet was tableted. The final weight of the contents was 2 g.

[Formulation Example 3] Preparation of granules

30% by weight of the extract of Example 1, 5% by weight of xylitol and 65% by weight of isomalt were mixed and granulated into granules using a fluidized bed granulator, and then filled in a capsule. The final weight of the contents was 2 g.

[Formulation Example 4] Preparation of injection

Injectables were prepared in a conventional manner according to the composition shown in Table 4 below.

Compounding ingredient content Example 1 10-50 mg Sterile sterilized water for injection Suitable amount pH adjusting agent Suitable amount

[Formulation Example 5] Health food

Healthy foods were prepared in a conventional manner according to the compositions shown in Table 5 below.

Compounding ingredient content Example 1 20 mg Vitamin A acetate 70 μg Vitamin E 1.0 mg Vitamin B1 0.13 mg Vitamin B2 0.15 mg Vitamin B6 0.5 mg Vitamin B12 0.2 μg Vitamin C 10 mg Biotin 10 μg Nicotinic acid amide 1.7 mg Folic acid 50 μg Calcium pantothenate 0.5 mg Ferrous sulfate 1.75 mg Zinc oxide 0.82 mg Magnesium carbonate 25.3 mg Potassium monophosphate 15 mg Dicalcium phosphate 55 mg Potassium citrate 90 mg Calcium carbonate 100 mg Magnesium chloride 24.8 mg

[Formulation Example 5] Health drinks

Healthy drinks were prepared in a conventional manner according to the composition shown in Table 6 below.

Compounding ingredient content Example 1 1000 mg Citric acid 1000 mg oligosaccharide 100g Taurine 1g Purified water Balance

Claims (12)

A composition for cell regeneration comprising an extract of ginseng flower as an active ingredient.
A composition for antiinflammation comprising an extract of ginseng flower as an active ingredient.
3. The method according to claim 1 or 2,
Wherein the extract is an extract which is extracted with water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol aqueous solution.
The method of claim 3,
Wherein the concentration of the aqueous C _{1 -} C ₅ alcohol solution is from 40% to 90% (v / v).
The method of claim 3,
Wherein the C ₁ -C ₅ alcohol is at least one selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol.
The method of claim 3,
Wherein the extract is an extract obtained by using an ethanol aqueous solution having a concentration of 60 to 80% (v / v).
3. The method according to claim 1 or 2,
Wherein the ginseng flower bud is removed from the ginseng fruit.
The method according to claim 1,
Wherein said composition is for wound healing.
The method according to claim 1,
Wherein the composition promotes skin regeneration.
3. The method according to any one of claims 1 to 2,
Wherein said composition is a health food or cosmetic composition.
3. The method according to any one of claims 1 to 2,
Wherein said composition is a pharmaceutical composition.
3. The method according to any one of claims 1 to 2,
The composition is formulated in the form of a powder, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and external preparations.

Images