KR20170064120A - A bio-chip for injecting liquid with the required amount - Google Patents

Abstract

The present invention relates to a bio-chip having a plurality of chambers and channels. More particularly, the present invention relates to a bio-chip in which a sample and a reagent can be injected in a plurality of chambers, The chamber-channel set consisting of a plurality of chambers and channels is made to coincide with the direction of the centrifugal force of the centrifugal separator so as to smooth the movement of the fluid and uniformly transfer the fluid to the plurality of chambers, So that the reaction conditions in the chamber-channel set of the biochip can be equally formed.

Description

A bio-chip for injecting liquid with the required amount

The present invention relates to a bio-chip having a plurality of chambers and channels. More particularly, the present invention relates to a bio-chip in which a sample and a reagent can be injected in a plurality of chambers, The chamber-channel set consisting of a plurality of chambers and channels is made to coincide with the direction of the centrifugal force of the centrifugal separator so as to smooth the movement of the fluid and uniformly transfer the fluid to the plurality of chambers, So that the reaction conditions in the chamber-channel set of the biochip can be equally formed.

An apparatus for inspecting or examining a substance present in a liquid sample such as blood or urine is called a diagnostic kit. In addition, tests or surveys conducted outside the hospital or laboratory are referred to as point-of-care testing (POCT).

In recent years, there has been a vigorous field investigation using such a diagnostic kit to conduct an inspection or investigation on a patient's site. In particular, rapid diagnostic tests, such as immunochromatographic assays, are used to identify diseases or detect changes in the healthcare field, and to quantitatively and quantitatively analyze trace amounts of analytes in a variety of fields, It is being developed in a simple way. In the field of healthcare, applications are also expanding to pregnancy, ovulation, infectious diseases, drug abuse, acute myocardial infarction, and cancer.

In this field investigation, a microfluidic device using a small amount of fluid is used. Such a microfluidic device is provided with a chamber in which a small amount of fluid can be confined and a channel through which the fluid can flow. Further, a valve means for controlling the flow of the fluid is provided. Particularly, a microfluidic device manufactured to perform a test including a biochemical reaction on a small chip is called a biochip. Such a biochip is made of glass, silicon or plastic, which enables a plurality of chambers and channels to be formed on a substrate to enable field investigation.

On the other hand, the biochip requires a driving pressure for transporting the fluid in the chamber to mix or react. For this driving pressure, capillary pressure may be used and pressure by a separate pump may be used. In recent years, as shown in FIG. 14, a disc-shaped biochip for driving a fluid by centrifugal force by rotating a disc-shaped biochip in which a chamber and a channel are disposed has been proposed. These are called Lab CD or Lab-on-a-CD. That is, LabCD and Lab-on-CD are centrifugal-based biochips.

FIG. 15 shows an example of a centrifugal force based biochip according to the prior art. As shown in the figure, in the conventional centrifugal force based biochip 1, the sample storage chamber 10 is disposed radially inward of the disk-shaped substrate 4. A sample injection port 8 for injecting a sample into the sample storage chamber 10 is formed on the upper surface of the sample storage chamber 10 do. And a reagent storage chamber 20 for receiving a predetermined amount of reagent is provided. The reagent storage chamber 20 may be provided with a plurality of reagent storage chambers 20 for storing different types and amounts of reagents. A plurality of reaction chambers 30 may be disposed outside the reagent storage chamber 20. The fluid in the sample storage chamber 10 and the reagent storage chamber 20 disposed in the substrate 4 flows into the reaction chamber 30 through the channel when the substrate 4 rotates about the central axis 5. [ ) And mixed.

However, in the conventional centrifugal force based biochip 1, one sample storage chamber 10 and one sample storage chamber 10 are provided on the circular substrate 4. A plurality of reagent storage chambers 20 and a plurality of reaction chambers 30 are provided and channels are formed therebetween which do not coincide with the direction 6 of the centrifugal force of the substrate 4. When the centrifugal force is applied, the moving speed and mixing degree of the fluid moving to the plurality of reaction chambers 30 are different from each other.

In addition, the biochip 1 of the conventional centrifugal force substrate must be provided with a sample and a reagent to be quantitatively injected into the sample storage chamber 10 and the plurality of reagent storage chambers 20 at a site outside a hospital or a laboratory. However, since it is difficult for unskilled persons to inject a sample and a reagent in a fixed amount in a poor environment, a sample or a reagent is not injected in a fixed amount, resulting in a difference in the result of the test, and contamination occurs during injection of a sample or a reagent .

(0001) Korean Patent No. 10-1257700 (Registered on March 31, 2013). (0002) Korean Patent Laid-Open No. 10-2015-010307 (Published on 2015.09.03)

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the conventional art, and it is a main object of the present invention to provide a method of arranging a plurality of sample storage chambers in a row, The present invention provides a biochip capable of injecting a sample easily even in the field because a sample can be transferred to all remaining sample storage chambers even when a sample is injected into the storage chamber.

It is another object of the present invention to provide a biochip that is capable of making a plurality of sample storage chambers, a reagent storage chamber, and a reaction chamber provided in a single biochip coincide with the direction of centrifugal force of a centrifuge so that the sample and reagent in the sample storage chamber and the reagent storage chamber The present invention provides a biochip capable of being quantitatively injected so that uniform reaction conditions can be formed in a plurality of mixing chambers by moving uniformly into a reaction chamber.

As a means for achieving the object of the present invention, a first embodiment of a bio-chip capable of being injected according to the present invention comprises:

In a biochip comprising a plate-shaped substrate having a constant thickness,

A plurality of sample storage chambers formed on one side of the substrate to store a sample;

A plurality of reagent storage chambers connected to the plurality of sample storage chambers to store reagents;

And a plurality of mixing chambers connected to the plurality of reagent storage chambers and mixing reagents and samples moving in the plurality of sample storage chambers and the reagent storage chambers,

A sample transfer channel formed between the sample storage chamber and the reagent storage chamber, and a reagent transfer channel formed between the reagent storage chamber and the mixing chamber are arranged in a straight line, and the plurality of sample storage chambers, the reagent storage chamber and the mixing chamber, Channel set of the chamber-channel sets, wherein the plurality of chamber-channel sets are formed to coincide with the direction of the centrifugal force of the centrifugal separator.

In the present invention, the substrate has a rectangular plate shape, and the chamber chamber-channel set consisting of the sample storage chamber, the reagent storage chamber, the mixing chamber, and the sample transfer channel and the reagent transfer channel is horizontally formed.

Wherein the plurality of sample storage chambers are formed near the rotation axis of the centrifuge; Wherein the plurality of mixing chambers are spaced apart from the rotation axis of the centrifuge; The plurality of reagent storage chambers are formed between the sample storage chamber and the mixing chamber.

On one side of the substrate, the sample storage chamber, the reagent storage chamber, and the mixing chamber are formed to a predetermined depth, and a sealing sheet for closing the sample storage chamber, the reagent storage chamber, and the opening portion of the mixing chamber is attached.

A sample inlet for injecting a sample into the sample storage chamber and a reagent inlet for injecting a reagent into the reagent storage chamber are formed on the other side of the substrate and a sealing sticker for closing the sample inlet and the reagent inlet Respectively.

The plurality of sample storage chambers are arranged in a line, and a channel for quantitation is formed between the plurality of sample storage chambers so that the sample in the sample storage chamber can move to the neighboring sample storage chamber.

Wherein the sample injection chamber is formed in the shape of a circular, elliptical, or arcuate shell surrounded by a vertical wall, the channel for quantification is formed in the middle part of the sample storage chamber, and the vertical wall surrounding the channel for quantitation is inclined in a funnel shape .

The cross-sectional area of the channel for quantification is formed to be relatively larger than the cross-sectional area of the sample injection channel.

The vertical wall around the quantitative channel is formed to be inclined so as to have an angle of 100 DEG or less centering on the quantitative channel.

The bio-chip has a rectangular plate shape having a bottom surface and a side surface. When the bio-chip is mounted on the centrifuge, the bottom surface of the bio-chip is set to coincide with the centrifugal force of the centrifuge.

The longitudinal direction of the sample storage chamber, the reagent storage chamber, and the mixing chamber of the biochip coincides with the direction of the centrifugal force of the centrifugal separator, thereby facilitating fluid movement in the sample storage chamber and the reagent storage chamber.

The lengths of the plurality of sample storage chambers, the reagent storage chambers and the mixing chambers of the biochip coincide with the directions of the centrifugal forces of the centrifugal separators so that the fluid in the plurality of sample storage chambers and the reagent storage chambers flows into the plurality of mixing chambers Move evenly.

The bio-chip is vertically installed on the inner surface of a cylindrical centrifuge.

In a second embodiment of the bio-chip capable of quantitation according to the present invention,

In a biochip comprising a plate-shaped substrate having a constant thickness,

A plurality of sample storage chambers formed on one side of the substrate to store a sample;

A plurality of reagent storage chambers formed on one side of the substrate to store reagents;

A plurality of sample storage chambers and a plurality of mixing chambers connected to the reagent storage chambers and mixing reagent and samples moving in the plurality of sample storage chambers and the reagent storage chambers,

The plurality of reagent storage chambers are formed in parallel with the plurality of sample storage chambers, the mixing chamber, and the sample transfer channel formed between the sample storage chamber and the mixing chamber, and are formed between the plurality of reagent storage chambers and the mixing chamber The reagent transfer channel is formed so as to be inclined at a constant angle.

The substrate is formed in a rectangular plate shape, and a chamber-channel set including the sample storage chamber, the mixing chamber, and the sample transfer channel is horizontally formed, and the reagent storage chamber is formed parallel to the chamber-channel set.

According to the bio-chip of the present invention, a plurality of chamber-channel sets including a plurality of chambers and channels are formed in one bio-chip, and the plurality of chamber-channel sets are all coincident with the direction of the centrifugal force of the centrifuge It is possible to carry out various tests and experiments simultaneously on one biochip.

The present invention also provides a method of forming a plurality of chamber-channel sets comprising a plurality of chambers and channels in a substrate, wherein the longitudinal direction of the plurality of chamber-channel sets is horizontally parallel to the direction of the centrifugal force of the centrifuge So that the movement of the fluid can be smoothly and uniformly performed. Thus, various tests and experiments can be performed at once using one biochip.

    In addition, since the sample injected into one sample storage chamber can be moved to all the sample injection chambers through the channel for quantitation formed between neighboring sample storage chambers, it is possible to easily inject samples into all the sample storage chambers It can be used conveniently for field inspection.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view showing an example of a biochip capable of being injected according to the present invention;
Fig. 2 is a front view of the bio-chip capable of being injected as shown in Fig. 1,
FIG. 3 is a cross-sectional view of a bio-chip capable of quantitation according to the present invention shown in FIG. 1,
FIG. 4 is an explanatory view showing a use state of a biochip capable of being injected according to the present invention;
FIG. 5 is an enlarged view showing a structure of a channel for quantification as a biochip capable of being injected according to the present invention,
FIG. 6 is an explanatory view showing a reagent injected into a plurality of reagent storage chambers of a biochip capable of being injected according to the present invention. FIG.
FIG. 7 is an explanatory view showing a state where a reagent is injected into a plurality of sample storage chambers of a biochip capable of being injected according to the present invention.
FIG. 8 is an explanatory view showing a bio-chip capable of being injected according to the present invention in a centrifuge; FIG.
9 is a plan view showing another embodiment of the bio-chip capable of being injected according to the present invention,
FIGS. 10 to 13 are explanatory views showing a state in which a fluid is injected into and transported to a bio-chip capable of being injected according to the present invention.
FIG. 14 is an explanatory view showing a centrifugal force-based biochip installed in a centrifuge according to the prior art;
15 is a plan view showing an example of a centrifugal force-based biochip according to the prior art.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of a "biochip capable of being injected in a quantitative manner" according to the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a perspective view showing an example of a biochip capable of being injected according to the present invention, FIG. 2 is a front view of the biochip capable of being injected as shown in FIG. 1, and FIG. 3 is a sectional view of the biochip capable of being injected as shown in FIG. 1 And FIG. 4 is an explanatory view showing the use state of the biochip capable of being injected according to the present invention.

1 to 3, a bio-chip 100 capable of being injected according to the present invention (hereinafter referred to as "bio-chip") includes a substrate 109 having a predetermined size, And a plurality of channels for transporting the fluid in the chamber.

First, the plurality of chambers include a sample storage chamber 110 for injecting and storing a sample, a reagent storage chamber 120 for injecting and storing the reagent, and a mixing chamber 130 for mixing the sample and the reagent .

The plurality of channels include a sample transfer channel 111 for transferring the sample in the sample storage chamber 110 and a reagent transfer channel 121 for transferring the sample in the sample storage chamber 120 .

As shown in the figure, the substrate 109 has a rectangular plate shape having a constant thickness. The substrate 109 may be a plastic injection molded product having a predetermined thickness, but the present invention is not limited thereto. The substrate 109 may be formed by laminating multiple layers of thin plates.

A plurality of sample storage chambers 110, a reagent storage chamber 102, a mixing chamber 103, a sample transfer channel 111 and a reagent transfer channel 121 are formed on one side of the substrate 109. The sample storage chamber 110, the reagent storage chamber 102, the mixing chamber 103, the sample transfer channel 111 and the reagent transfer channel 121 are formed on one side of the substrate 109 with a predetermined depth .

Preferably, the sample storage chamber 110, the reagent storage chamber 102, the mixing chamber 103, the sample transfer channel 111 and the reagent transfer channel 121 are formed at one side of the substrate 109 at a constant height Can be formed inside the space surrounded by the protruding vertical wall (105). However, the present invention is not limited thereto.

As shown in FIG. 3, a sealing sheet 140 is further provided on one side of the substrate 109. The seal sheet 140 has an area similar to that of the substrate 109 and includes a sample storage chamber 110, a reagent storage chamber 102, a mixing chamber 103, a sample transfer channel 111, Is bonded to the upper surface of the vertical wall 105 forming the channel 121 to close the openings of the plurality of chambers and channels.

A sample inlet 115 for injecting a sample into the plurality of sample storage chambers 110 and a reagent injector for injecting reagents into the plurality of reagent storage chambers 120 are formed on the other side of the substrate 109, An injection port 215 is provided. A vertical wall 105 is formed around the sample injection port 115 and the reagent injection port 125 so as to surround the sample injection port 115 and the reagent injection port 125. A sealing sticker 150 is further provided on the other side of the substrate 109. The sealing sticker 150 is attached to the upper surface of the sample injection port 115 and the vertical wall 105 of the reagent injection port 125 to close the sample injection port 115 and the reagent injection port 125.

The sample storage chamber 110, the reagent storage chamber 102, the mixing chamber 103, the sample transfer channel 111, and the reagent transfer channel 121, which are engraved on one side of the substrate 109, And a sealing sticker 150 is attached to the sample injection port 115 and the reagent injection port 125 formed on the other side of the substrate 109, The inside of the storage chamber 110, the reagent storage chamber 102 and the mixing chamber 103 are sealed and connected to each other through the sample transfer channel 111 and the reagent transfer channel 121.

2, the biochip 100 of the present invention includes the sample storage chamber 110, the reagent storage chamber 120, the mixing chamber 103, the sample transfer channel 111, and the reagent transfer channel 121 And a chamber-channel set 160 made up of a plurality of chamber-channel sets 160. The chamber-channel set 160 is a unit structure for carrying out one experiment or test. That is, when a sample is injected into the one sample storage chamber 110, the reagent is injected into the one reagent storage chamber 120, and the reagent is transferred to one mixing chamber 130 and mixed, Can be performed.

In addition, the biochip 100 of the present invention includes a plurality of chamber-channel sets 160. That is, the plurality of chamber-channel sets 160 can perform various kinds of tests and experiments at one time. That is, a sample is injected in a predetermined amount into the plurality of sample storage chambers 110, different kinds and amounts of reagents are injected into the plurality of reagent storage chambers 120, And then mixed and mixed, it is possible to carry out various experiments or tests at a time.

In order to perform an experiment or a test using the biochip 100 of the present invention, the fluid in the sample storage chamber 110 and the reagent storage chamber 120 must be moved to the mixing chamber 130. As shown in FIG. 4, the centrifugal force of the centrifugal separator 101, which rotates about the rotation axis 103, is used for this purpose. That is, the fluid in the chamber is transferred using the centrifugal force generated in the radial direction when the centrifuge 101 rotates about the rotation axis 103.

In order to smoothly move the fluid in the sample storage chamber 110 and the reagent storage chamber 120 to the mixing chamber 130 using the centrifugal force of the centrifugal separator 101, Is formed such that the longitudinal direction of the chamber-channel set 160 coincides with the direction 104 of the centrifugal force of the centrifugal separator 101. In order to uniformly move the fluids in the plurality of sample storage chambers 110 and the reagent storage chambers 120 to the plurality of mixing chambers 130 using the centrifugal force of the centrifugal separator 101, The biochip 100 is formed such that the longitudinal directions of the plurality of chamber-channel sets 160 coincide with the centrifugal direction 104 of the centrifugal separator 101.

To this end, the sample storage chamber 110 and the reagent storage chamber 120, the mixing chamber 130, the sample transfer channel 111, and the reagent transfer channel (not shown) constituting the one chamber- 121 are arranged in a line. And the longitudinal direction of the chamber-channel set 160 arranged in a row is formed horizontally so as to coincide with the direction 104 of the centrifugal force of the centrifuge 101.

The reagent storage chamber 120, the mixing chamber 130 and the plurality of sample transfer channels 111 and the reagent transfer channels 121 are arranged in a row do. That is, the longitudinal direction of the plurality of chamber-channel sets 160 is formed horizontally so as to coincide with the direction 104 of the centrifugal force of the centrifugal separator 101. The direction perpendicular to the longitudinal direction of the plurality of chamber-channel sets 160, that is, the vertical direction, should be perpendicular to the rotation axis 103 of the centrifugal separator 101.

The sample storage chamber 110 is located closest to the rotation axis 103 of the centrifugal separator 101 and the mixing chamber 130 is located at a position farthest from the rotation axis 103 of the centrifugal separator 101 And the reagent storage chamber 120 is formed between the sample storage chamber 110 and the mixing chamber 130. Therefore, when centrifugal force acts on the biochip 100, the fluid in the sample storage chamber 110 and the reagent storage chamber 120 is moved to the mixing chamber 130.

The plurality of sample storage chambers 110 are vertically formed near the rotation axis 103 of the centrifugal separator 101 and the plurality of mixing chambers 130 are connected to the rotation axis of the centrifugal separator 101 103, and the plurality of reagent storage chambers 120 are vertically formed in the middle thereof. Therefore, when the centrifuge 101 rotates, the samples in the plurality of sample storage chambers 110 move to the plurality of mixing chambers 130 through the plurality of reagent storage chambers 120, The reagents in the storage chamber 120 are uniformly moved to the plurality of mixing chambers 130 so that the same reaction conditions are formed in the plurality of mixing chambers 130.

4, the bottom surface of the bio-chip 100 is connected to the centrifugal separator 101 through the centrifugal separator 101 so that all the chamber-channel sets 160 are aligned with the centrifugal force direction 104 of the centrifugal separator 101. [ The side surface of the bio-chip 100 should be installed vertically so as to be parallel to the rotation axis 103 of the centrifugal separator 101. That is, if the biochip 100 is installed on the plane of the centrifugal force 104 of the centrifugal separator 101 and the rotation axis 103, the plurality of chamber- And coincides with the direction 104 of the centrifugal force of the separator 101.

When the biochip 100 according to the present invention is vertically installed on the centrifugal separator 101, the plurality of chamber-channel sets 160 are aligned with the centrifugal direction 104 of the centrifugal separator 101, The sample and reagents in the sample storage chamber 110 and the reagent storage chamber 120 are uniformly moved to the plurality of mixing chambers 130 so that the reaction conditions in all the mixing chambers 130 are the same.

That is, since the centrifugal forces acting on the sample storage chamber 110 and the reagent storage chamber 120 are the same, the movement speed and the movement amount of the fluid moving to the plurality of reaction chambers 130 are the same and the same, do. If the movement speed and the movement amount of the fluid moving to the plurality of mixing chambers 130 are different from each other, the experimental conditions in each mixing chamber 130 are not the same.

As described above, according to the first aspect of the present invention, the sample storage chamber 110, the reagent storage chamber 120, the mixing chamber 130, and the sample transfer channel 111 and the reagent transfer channel Channel set 160 comprises at least one chamber-channel set 160 consisting of a plurality of chamber-channel sets 112 which are aligned in a horizontal direction so as to coincide with the centrifugal direction 104 of the centrifuge 101 So that the fluid in the chambers 110 and 120 is smoothly moved.

A second aspect of the present invention is to provide a method of manufacturing a semiconductor device including a plurality of sample storage chambers 110, a reagent storage chamber 120, a mixing chamber 130 and a plurality of sample transfer channels 111 and a reagent transfer channel Channel set 160 in which the plurality of chamber-channel sets 160 are all aligned parallel to the direction 104 of the centrifugal force of the centrifugal separator 101. The plurality of chamber- The fluid in the plurality of chambers 110 and 120 may be uniformly moved to the plurality of mixing chambers 130 so that a plurality of experiments or tests can be performed at one time using one biochip 100 .

The third aspect of the present invention is that the bottom surface of the bio-chip 100 having the plurality of chambers 110, 120 and 130 and the plurality of channels 111 and 121 is connected to the centrifuge 101, The side surface of the bio-chip 100 is provided in parallel with the rotation axis 103 of the centrifugal separator 101 and the longitudinal direction of the plurality of chamber-channel sets 160 is parallel to the direction 104 of the centrifugal force of the chamber- (104) of the centrifugal force of the centrifugal separator (101).

A fourth aspect of the present invention is a method for separating a plurality of sample storage chambers 110 and a reagent storage chamber 120 from each other and allowing a skilled person to quantitatively inject a reagent in a hospital or a laboratory in the plurality of reagent storage chambers 120 And the plurality of sample storage chambers 110 can be easily dosed by a non-expert in the field so that they can be conveniently used in the field inspection.

More specifically, as shown in FIG. 5, the biochip 100 is further provided with a channel 112 for quantitation between a plurality of sample storage chambers 110 vertically arranged vertically. The channel for quantification 112 provides a path through which the sample injected into one of the sample storage chambers 110 can be moved to another sample storage chamber 110 adjacent thereto. Accordingly, even if a sample is injected into any one of the sample storage chambers 110, the sample can be injected into all of the sample storage chambers 110 in a predetermined amount.

As shown in the figure, the sample storage chamber 110 is formed in a circular, elliptical, or abacus-like shape. The sample storage chamber 110 is surrounded by a vertical wall 105 having a predetermined height, and the quantitative channel 112 is formed by opening a part of the vertical wall 105. The channel for quantification 112 is formed at a point where neighboring sample storage chambers 110 meet with each other.

The vertical walls 105a and 105b around the quantitation channel 112 are inclined at a predetermined angle so that a funnel shape is formed around the quantitation channel 112. Preferably, an angle between the vertical walls 105a and 105b around the quantitation channel 112 is 100? Or less. Thus, the sample passing through the channel for quantitation 112 is prevented from bubbling and can move smoothly. The cross-sectional area of the channel for quantification 112 is preferably larger than the cross-sectional area of the sample transfer channel 111. For example, when the sample injection port 125 of the sample storage chamber 120 is sealed when the cross-sectional area of the sample transfer channel 111 is larger than the cross-sectional area of the channel for quantification 112, the sample storage chamber 110 Is moved to the reagent storage chamber 120. [0064] The cross-sectional area of the sample transfer channel 111 is preferably at least 1 mm < 2 > to increase the surface tension. That is, if the cross-sectional area of the sample transfer channel 111 is small, the surface tension of the fluid is increased, and the bio-chip 100 is vertically erected so that the sample does not flow downward.

6 and 7, only two sample injection ports 115 for injecting the sample into the plurality of sample storage chambers 110 may be formed. Preferably, the sample injection ports 110 are formed at both ends of the plurality of sample storage chambers 110. That is, the biochip 100 according to the present invention has the channels 112 for quantitation formed between the neighboring sample storage chambers 110, so that the sample is injected into any one of the sample storage chambers 110, When air is discharged through the chamber 110, the sample injected into the sample storage chamber 110 is transferred to all the sample storage chambers 110 and injected in a predetermined amount.

As described above, since the biochip 100 can continuously inject samples into one sample storage chamber 110 and inject the samples into all of the sample storage chambers 110 in a quantitative manner, a non-expert can easily inject the sample in a predetermined amount . Therefore, in the field investigation of the poor condition, the unskilled person can easily perform field inspection using the biochip 100 of the present invention.

Hereinafter, a method of using the biochip 100 according to the present invention will be described.

6, the biochip 100 is horizontally laid down and a plurality of reagent storage chambers 125 are formed through a plurality of reagent injection ports 125 formed in the biochip 100 by using a tool such as a pipette P, (120). At this time, since different reagents are injected into the reagent storage chamber 120, it is preferable that the reagent is injected by a skilled person in a hospital or a laboratory. Then, a sealing sticker 150 is attached to the reagent inlet 125 and sealed.

Then, as shown in FIG. 10, the reagent injected into the reagent storage chamber 120 is stored in the reagent storage chamber 120. That is, since a positive pressure is formed in the sample storage chamber 110 and the mixing chamber 130 in a state where all of the sample injection port 115 and the reagent injection port 125 are sealed, The reagent in the chamber 120 does not move to the sample storage chamber 110 or the mixing chamber 130. For this purpose, the reagent storage chamber 120 must have a sufficiently small cross-sectional area, and the sample storage chamber 110 and the mixing chamber 130 must be sealed. The reagent injected into the biochip 100 may be stored in a refrigerator to prevent deterioration or may be freeze-dried in a state where a reagent is injected into the reagent storage chamber 120.

Subsequently, in the field, a sample collected from a person or an animal is injected into a plurality of sample storage chambers 110 of the biochip 100 in a fixed amount. 7, the bio-chip 100 may be laid horizontally, the sealing stickers 150 attached to the two sample injection ports 115 may be removed, and then the bio-chip 100 may be removed using a tool such as a pipette P And the sample is injected through one sample inlet 115.

11, the sample injected into one of the sample storage chambers 110 moves to the neighboring sample storage chamber 110 through the channel 112 for quantitation. As shown in FIG. 12, when all the sample storage chambers 110 are filled with the sample, the same amount of sample is injected into all the sample storage chambers 110. As described above, since the biochip 100 of the present invention can easily inject a sample into a plurality of sample storage chambers 110 in a predetermined amount, the sample can be easily injected by a non-expert in a poor environment. Next, when the two sample injection ports 115 are sealed with the sealing sticker 150, the sample injected into the plurality of sample storage chambers 110 is stored in the sample storage chamber 110. At this time, since the sample transfer channel 111 formed in the sample storage chamber 110 has a very small cross-sectional area, the sample transfer channel 111 serves as a valve for preventing the sample in the sample storage chamber 110 from moving to the reagent storage chamber 120 .

As described above, the biochip 100 into which the sample and the reagent are both injected can be mounted on the centrifugal separator 101 as shown in FIG. 8 for testing or experiments. The centrifugal separator (101) is made of a cylinder having a size capable of accommodating at least two or more bio-chips (100). The centrifugal separator 10 includes a motor (not shown) which can rotate the cylindrical body about the rotation axis 103. The rest of the configuration is well known in the art, and a detailed description thereof will be omitted.

As shown in the figure, the biochip 100 is vertically installed on the inner surface of the cylinder. To this end, a coupling groove 141 for inserting the bio-chip 100 is vertically formed on the inner surface of the centrifugal separator 101. Further, a fixing groove 143 for fixing the upper and lower positions of the bio-chip 100 is further formed at the center of the coupling groove 141. However, the centrifugal separator 101 of the present invention is not limited to this configuration. The bottom surface of the bio-chip 100 may be aligned with the centrifugal direction 104 of the centrifugal separator 101, May be any of those provided parallel to the rotation axis 103 of the centrifugal separator 101.

An engaging protrusion 108 corresponding to the engaging groove 141 is formed on the other side of the bio-chip 100, that is, on the side adjacent to the plurality of mixing chambers 130. A fixing protrusion 107 corresponding to the fixing groove 143 is formed in the center of the engaging protrusion 108. Further, a projection (not shown) may be formed on the opposite side of the fixing groove 143, and a groove 106 corresponding to the projection may be formed on the bio-chip 100.

Therefore, the coupling protrusion 108 of the bio-chip 100 is inserted into the coupling groove 141 of the centrifugal separator 101, and the fixing protrusion 106 of the bio-chip 100 is inserted into the fixing groove 143 The side surface of the biochip 100 is parallel to the rotation axis 103 of the centrifugal separator 101 and the bottom surface of the biochip 100 coincides with the centrifugal direction 104 of the centrifugal separator 101 . The longitudinal direction of the plurality of chamber-channel sets 160 provided in the biochip 100 coincides with the direction 104 of the centrifugal force of the centrifugal separator 101 so that all of the sample storage chamber 110 and the reagent storage chamber 110, The fluid moving in the mixing chamber 130 moves equally to the plurality of mixing chambers 130 so that the reaction conditions of all the mixing chambers 130 are the same.

13, after the biochip 100 is mounted on the centrifuge 101, the centrifuge 101 is rotated about the rotation axis 103, so that the plurality of sample storage chambers 110, The sample stored in the plurality of reagent storage chambers 120 moves to the plurality of mixing chambers 130 through the plurality of reagent storage chambers 120 and the reagent stored in the plurality of reagent storage chambers 120 flows into the plurality of mixing chambers 130 And mixed in the plurality of mixing chambers 130.

9 is a plan view showing another embodiment of the bio-chip 200 capable of being injected according to the present invention. Two reagent storage chambers 220a and 220b are formed between the sample storage chamber 210 and the mixing chamber 230. The sample storage chamber 210 is connected to the mixing chamber 230, And the two reagent storage chambers 220a and 220b are connected to the mixing chamber 230, respectively. That is, in this embodiment, different kinds of reagents are injected into the two reagent storage chambers 220a and 220b, and various experiments and tests can be performed. At this time, the two reagent storage chambers 220a and 220b are formed to coincide with the centrifugal force direction 104 of the centrifugal separator 101. For this purpose, the two reagent storage chambers 220a and 220b are formed in parallel up and down. Also, the reagent storage chambers 220a and 200b are not limited to two chambers, and two or more reagent storage chambers may be formed as long as the reagent storage chambers 220a and 200b can coincide with the centrifugal force direction 104 of the centrifuge 101.

In this way, the present invention is characterized in that at least one chamber-channel set 160 comprising a plurality of chambers and channels is formed on one substrate 109, and the longitudinal direction of the plurality of chamber- Parallel to the direction 104 of the centrifugal force of the separator 101 so that the fluid is smoothly and uniformly moved so that various tests or experiments can be performed simultaneously using one biochip 100 have.

In addition, the present invention is characterized in that a channel 112 for quantitation is formed between neighboring sample storage chambers 110 and a sample is injected into any one of the sample storage chambers 110, So that it is possible to easily inject a sample into a non-skilled person so that it can be conveniently used for on-site inspection.

It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the following claims . It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

100: Biochip 101: Centrifuge
102: rotating shaft 103: direction of rotating shaft
104: direction of centrifugal force 106: vertical wall
107: fixed protrusion 108: engaging protrusion
109: substrate 110: sample storage chamber
111: sample transport channel 112: channel for quantification
115: Sample inlet 120: Reagent storage chamber
121: Reagent transfer channel 125: Reagent inlet
130: mixing chamber 141: engaging groove
143: Fixing groove

Claims (19)

In a biochip comprising a plate-shaped substrate having a constant thickness,
A plurality of sample storage chambers formed on one side of the substrate to store a sample;
A plurality of reagent storage chambers connected to the plurality of sample storage chambers to store reagents;
And a plurality of mixing chambers connected to the plurality of reagent storage chambers and mixing reagents and samples moving in the plurality of sample storage chambers and the reagent storage chambers,
A sample transfer channel formed between the sample storage chamber and the reagent storage chamber, and a reagent transfer channel formed between the reagent storage chamber and the mixing chamber are arranged in a straight line, and the plurality of sample storage chambers, the reagent storage chamber and the mixing chamber, Wherein the plurality of chamber-channel sets are formed to coincide with the direction of the centrifugal force of the centrifugal separator. The method according to claim 1,
Wherein the substrate has a rectangular plate shape, and the chamber-channel set including the sample storage chamber, the reagent storage chamber, the mixing chamber, and the sample transfer channel and the reagent transfer channel is horizontally formed. 3. The method of claim 2,
Wherein the plurality of sample storage chambers are formed near the rotation axis of the centrifuge; Wherein the plurality of mixing chambers are spaced apart from the rotation axis of the centrifuge; Wherein the plurality of reagent storage chambers are formed between the sample storage chamber and the mixing chamber. The method of claim 3,
The sample storage chamber, the reagent storage chamber, and the mixing chamber are formed at a predetermined depth on one side of the substrate, and a sealing sheet for closing the sample storage chamber, the reagent storage chamber, and the opening portion of the mixing chamber is attached A biochip that can be injected as a feature. 5. The method of claim 4,
A sample inlet for injecting a sample into the sample storage chamber and a reagent inlet for injecting a reagent into the reagent storage chamber are formed on the other side of the substrate and a sealing sticker for closing the sample inlet and the reagent inlet Wherein the biochip is attached to the biochip. 6. The method according to any one of claims 1 to 5,
Wherein the plurality of sample storage chambers are arranged in a line, and between the plurality of sample storage chambers, a channel for quantitation is formed so that the sample in the sample storage chamber can move to a neighboring sample storage chamber. The method according to claim 6,
Wherein the sample injection chamber is formed in the shape of a circular, elliptical, or arcuate shell surrounded by a vertical wall, the channel for quantification is formed in the middle part of the sample storage chamber, and the vertical wall surrounding the channel for quantitation is inclined in a funnel shape Wherein the bio-chip is formed of a metal. The method according to claim 6,
Wherein the cross-sectional area of the channel for quantification is relatively larger than the cross-sectional area of the sample injection channel. 8. The method of claim 7,
Wherein the vertical wall around the channel for quantification is inclined so as to have an angle of less than 100 DEG with respect to the channel for quantification. 6. The method according to any one of claims 1 to 5,
Wherein the bio-chip has a rectangular plate shape having a bottom surface and a side surface, and the bottom surface of the bio-chip is installed so as to coincide with the centrifugal force of the centrifuge when the bio-chip is mounted on the centrifuge. Biochip. 11. The method of claim 10,
Wherein the longitudinal direction of the sample storage chamber, the reagent storage chamber, and the mixing chamber of the biochip coincides with the direction of the centrifugal force of the centrifugal separator so that fluid movement in the sample storage chamber and the reagent storage chamber is smoothly performed. Possible biochip. 11. The method of claim 10,
The lengths of the plurality of sample storage chambers, the reagent storage chambers, and the mixing chambers of the biochip coincide with the directions of the centrifugal forces of the centrifugal separators so that the fluid in the plurality of sample storage chambers and reagent storage chambers flows into the plurality of mixing chambers Wherein the biochip is uniformly moved. 13. The method of claim 12,
Wherein the bio-chip is vertically installed on an inner surface of a cylindrical centrifugal separator. In a biochip comprising a plate-shaped substrate having a constant thickness,
A plurality of sample storage chambers formed on one side of the substrate to store a sample;
A plurality of reagent storage chambers formed on one side of the substrate to store reagents;
A plurality of sample storage chambers and a plurality of mixing chambers connected to the reagent storage chambers and mixing reagent and samples moving in the plurality of sample storage chambers and the reagent storage chambers,
The plurality of reagent storage chambers are formed in parallel with the plurality of sample storage chambers, the mixing chamber, and the sample transfer channel formed between the sample storage chamber and the mixing chamber, and are formed between the plurality of reagent storage chambers and the mixing chamber Wherein the reagent transfer channel is inclined to a predetermined angle. 15. The method of claim 14,
The substrate is formed in a rectangular plate shape, and a chamber-channel set including the sample storage chamber, the mixing chamber, and the sample transfer channel is horizontally formed, and the reagent storage chamber is formed parallel to the chamber- Wherein the biochip is capable of being injected. 16. The method of claim 15,
The sample storage chamber, the reagent storage chamber, and the mixing chamber are formed at a predetermined depth on one side of the substrate, and a sealing sheet for closing the sample storage chamber, the reagent storage chamber, and the opening portion of the mixing chamber is attached A biochip that can be injected as a feature. 17. The method of claim 16,
A sample inlet for injecting a sample into the sample storage chamber and a reagent inlet for injecting a reagent into the reagent storage chamber are formed on the other side of the substrate and a sealing sticker for closing the sample inlet and the reagent inlet Wherein the biochip is attached to the biochip. 18. The method of claim 17,
Wherein the plurality of sample storage chambers are arranged in a line, and between the plurality of sample storage chambers, a channel for quantitation is formed so that the sample in the sample storage chamber can move to a neighboring sample storage chamber. 19. The method according to any one of claims 14 to 18,
Wherein the bio-chip has a rectangular plate shape having a bottom surface and a side surface, and when the bio-chip is mounted on the centrifuge, the bottom surface of the bio-chip is set to coincide with the centrifugal force of the centrifuge. Biochip.

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