KR20160080407A - Extract drink from soybean germination and manufacturaring process for the same - Google Patents

Abstract

The present invention relates to a soybean germinated beverage and a method for producing the beverage, and in the method for producing a soybean germinated beverage according to an embodiment of the present invention, soybeans are dipped in water for 10 to 14 hours, Germinating for 18 to 24 hours; Wet-milling the germinated soybeans; Separating the wet pulverized phosphorus and filtrate to obtain a filtrate; And extracting the extracted beverage with the filtrate, wherein the soybean germinated extract beverage according to the present invention has a high content of isoflavone.

Description

[0001] The present invention relates to a soybean germ extract drink and a preparation method thereof,

The present invention relates to a soybean germinated extract beverage and a method for producing the same, and more particularly, to a soybean germinated beverage having a high content of isoflavones by using a germination process of soybeans and a method for producing the same.

Due to the development of modern science, beyond the basic purpose of satisfying the nutritional supply and palatability of food, there are active researches on functional food having the effect of preventing and healing diseases.

In particular, there is a fierce competition among companies in order to preempt health - functional foods that help menopausal health. According to the Food and Drug Administration's report on the production of dietary supplements in 2013, the output of various natural compound extracts was 7 billion times as high as 70 billion won in one year, which was 10 billion won in 2012. As such, the related markets are growing rapidly, and the number of participating companies is increasing.

A variety of studies and related products for health-functional foods using soybeans, which are known to be good for women's menopause, are being launched.

Soybeans are rich in protein and fat, and they have been a big part of the Korean diet since ancient times. It has been found that soy has nutritional superiority of food, and also contains various functional physiologically active ingredients such as isoflavone, saponin, lecithin and oligosaccharide in a large amount.

Isoflavones among the functional components contained in soybean are classified as phytoestrogens because they exhibit weak estrogenic activity in mammals. They are used to prevent and treat menopausal symptoms of menopausal women, to prevent and treat menstrual syndrome, to treat osteoporosis and senility It is known that there is a great mental effect such as prevention and treatment of fracture, anticancer effect on breast cancer and prostate cancer, prevention of vascular disease diseases such as heart disease, hypertension and arteriosclerosis and prevention of aging of postmenopausal women and decline of memory and concentration.

As a result, consumption of soybeans has increased significantly in western countries, which is only about 1 / 20th to 1/50 of the intake of Asian people.

Korean Patent Laid-Open Publication No. 2013-0057817 discloses a composition for prevention and improvement of hypertension and thrombosis, which comprises an actinic sweet soybean extract containing isoflavone as an active ingredient.

Korean Patent Publication No. 2011-0027247 discloses a method for enhancing the activity of isoflavone contained in whole soybean milk by fermenting soybean oil with lactic acid bacteria having high beta-glucosidase activity extracted from kimchi when whole soybean milk is produced have.

An object of one embodiment of the present invention is to provide a soybean germinated beverage having a high content of isoflavones by using a germination process of soybeans and a method for producing the same.

One embodiment of the present invention comprises: dipping soybeans in water for 10 to 14 hours and germinating at a temperature of 30 to 35 DEG C for 18 to 24 hours; Wet-milling the germinated soybeans; Separating the wet pulverized phosphorus and filtrate to obtain a filtrate; And a step of preparing an extracted beverage by the filtrate. The present invention also provides a method for producing a soybean germinated beverage.

In the germinating step, the soybeans are immersed in 0.1 N to 3N NaCl solution and germinated.

The step of separating the corpuscle and the filtrate may further include the step of adding water to the first separated soybean flour, mixing and then separating the filtrate again.

The germinating step may be performed while the black cloth is covered with the soaked soybeans.

The step of preparing the extracted beverage may include mixing the physiologically active substance with the filtrate.

Another embodiment of the present invention provides a soybean germinated extract beverage which is produced by the above method.

The soybean germinated beverage according to one embodiment of the present invention has a high content of isoflavone by using the germination process of soybeans.

In particular, the contents of genistin, daidzin, and glycitin, which are glycosides, were significantly increased, and the contents of non-glycosides genistein, daidzein, and glycitein (Glycitein) was also increased. The soybean germinated beverage according to one embodiment of the present invention is effective in improving female menopausal and premenstrual syndrome, and is expected to have an anticancer effect and an improvement in vascular disease.

1 is a chromatogram showing Daidzin measurement results of soybeans germinated with water, 0.1N NaCl, and 3N NaCl solutions, respectively.
2 is a chromatogram showing the results of Glycitin measurement of soybeans germinated with water, 0.1N NaCl, and 3N NaCl solutions, respectively.
3 is a chromatogram showing the results of Genistin measurement of soybeans germinated with water, 0.1N NaCl, and 3N NaCl solutions, respectively.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments.

Throughout the description of the present invention, when a component is referred to as " comprising ", it means that it can include other components as well, without excluding other components unless specifically stated otherwise.

In the specification of the present invention, it is to be understood that when a step is located "on" or "before" another step, this is not only the case where a step is in a direct time series relationship with another step, And may have the same rights as in the case of an indirect temporal relationship in which the temporal order of the two phases can be changed.

The terms " about "," substantially ", etc. used to the extent that they are used throughout the specification of the present invention are used in their numerical values or in close proximity to their numerical values when the manufacturing and material tolerances inherent in the meanings mentioned are presented, Is used to prevent unauthorized exploitation by an unscrupulous infringer of precise or absolute disclosures in order to aid in the understanding of the disclosure. The term " step " or " step of ~ " used throughout the specification does not mean " step for.

The present invention relates to a soybean germinated beverage and a method for producing the beverage, and in the method for producing a soybean germinated beverage according to an embodiment of the present invention, soybeans are dipped in water for 10 to 14 hours, Germinating for 18 to 24 hours; Wet-milling the germinated soybeans; Separating the wet pulverized phosphorus and filtrate to obtain a filtrate; And extracting the extracted beverage by the filtrate, wherein the soybean germinated extract beverage according to an embodiment of the present invention is manufactured by the above method.

The method of manufacturing a soybean germinated beverage according to an embodiment of the present invention may be characterized in that the content of isoflavone is high using a germination process of soybean. In particular, the content of Genistin, Daidzin, and Glycitin is high, and it is expected that the extracted beverage will be effective for the improvement of female menopausal period and premenstrual syndrome, and for improving the anticancer effect and vascular disease.

Soy isoflavones are present in the form of the glycosides genistin, daidzin, glycitin, and the aglycone genistein, daidzein, Glycitein, and many other derivatives.

Isoflavone is a representative plant estrogen that works similarly to estrogen, a female hormone. When women become menopausal, estrogen levels decrease by up to 30%, resulting in a variety of menopausal symptoms. Menopausal women taking isoflavones may improve menopausal symptoms such as depression, nervousness, and sweating.

Isoflavones can also help prevent and treat premenstrual syndrome. About 75% of pregnant women have physical and psychological premenstrual syndrome. 3 ~ 10 days before menstruation It is typical symptom such as bloating of the lower abdomen, weight gain, tension, headache, and even psychological changes such as depression and suicidal impulse may appear. In case of premenstrual syndrome, there is no definite cause and treatment method. It is being investigated that ingestion of soy isoflavones may induce changes in menstrual cycle to prevent premenstrual syndrome and relieve symptoms.

In addition, osteoporosis is a problem due to the increase in calcium consumption due to pregnancy and childbirth of women and the decrease of estrogen after menopausal period. However, since soy isoflavone has estrogen-like activity in skeletal metabolism, effective.

In addition, Asian people who consume enough soybeans have been reported to have a significantly lower mortality rate from breast cancer and prostate cancer compared to Westerners. Therefore, it is known that Asian people have decreased cholesterol and LDL and increase HDL to prevent vascular diseases It is known.

Hereinafter, each step of the method of manufacturing a soybean germinated extract beverage according to an embodiment of the present invention will be described in detail, and the soybean germinated extract beverage in one embodiment of the present invention can be more specifically identified and understood.

First, the step of immersing the soybeans in water for 10 to 14 hours and germinating at a temperature of 30 to 35 DEG C for 18 to 24 hours can be performed.

The soybeans can be soaked in water for 10 to 14 hours after being washed, and they can be germinated at a temperature of 30 to 35 DEG C for 18 to 24 hours.

It is judged that the content of isoflavone is changed in the process of germinating soybeans. By extracting soybeans after germination, an extract having a high content of isoflavones can be obtained.

According to one embodiment of the present invention, the germinating step may be performed by covering the soybean with a black cloth so as to block the light, thereby germinating.

Further, according to one embodiment of the present invention, the soybean germination can be germinated with a 0.1N to 3N NaCl solution. When germinated in NaCl solution, the content of isoflavone may increase due to stress.

Next, the germinated soybean can be wet-pulverized.

According to one embodiment of the present invention, the germinated soybean can be wet-milled, but it is possible to use a wet mill which is commonly used in the art. The screw speed during wet milling may be 3 to 5 rpm.

Next, the wet pulverized product is separated into a soybean sponge and a filtrate to obtain a filtrate.

When the soybean germinated as described above is wet pulverized, the soybean meal and the filtrate are mixed, but the soybean meal and the filtrate can be separated. A method for separating the filtrate is not particularly limited, and for example, a filter can be used.

Water can be added again to the firstly separated soymilk, and the filtrate can be separated again after mixing. This separation process can be performed about two to three times.

Next, the extracted beverage can be prepared by the filtrate obtained above.

In the form of a juice drink, the filtrate can be taken intact and mixed with a physiologically active substance used in beverage production to improve flavor and palatability, so that it can be made into an extract beverage. The method for producing the extracted beverage is not particularly limited, and a method commonly used in the art can be used. For example, the filtrate, juice, and other physiologically active substances may be mixed and stored in a refrigerator to be taken as an extract beverage.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.

[Example]

The seeds grown in the Andong area were collected and 100 g of the seeds were washed with water and immersed in 300 ml of water for 12 hours. The soaked soybean beans were germinated by covering with black cloth at 32 ° C for 24 hours. The germinated soybeans were wet pulverized and separated into a soybean meal and a filtrate. Water was added to the separated marjoram, and the marjoram and the filtrate were further separated. One part of water was added to the separated marjoram, and the filtrate was further separated. The collected filtrate was concentrated to 50% and analyzed by GC (Gas Chromatography). Purified isoflavones were prepared as beverages.

The filtrate was collected and concentrated in the same manner as above except that the beans were immersed and germinated in 0.1 N NaCl or 3 N NaCl solution, and the content of isoflavone was analyzed by GC (Gas Chromatography).

[evaluation]

The content of isoflavone by gas chromatography was measured by the following method.

1) Analytical sample pretreatment method

10 g of the sample was taken in a 250 ml Erlenmeyer flask, and 40 ml of 80% methanol was added and extracted in a dark state at 65 ° C for 2 hours in a constant temperature water bath. After cooling, 3 ml of 2M sodium hydroxide solution was added, and the mixture was stirred at room temperature for 10 minutes. Then 1 ml of acetic acid was added, and 80 ml of methanol was mixed with 50 ml. 4 ml of distilled water was added to 5 ml of the mixed sample filtrate, and 10 ml of methanol was used. After centrifugation, the supernatant was filtered with a 0.45um Mbrene filter.

2) Measurement conditions

end. Equipment used: Agilent 1100 Series

(1) Pump: G1312A Binpump

(2) Detector: G1315B DAD

(3) Autosampler: G1329A ALS

I. Analysis conditions:

(1) Flow rate: 0.8 ml / min

(2) Mobile phase: Isocratic

(3) Column: CAPCELL-Pak C-18, 5 占 퐉, 4.6 占 250 mm (Shiseido)

(4) Column Temp .: 30 DEG C

(3) Detection wavelength: 250 nm

3) Measurement result

1 is a chromatogram showing Daidzin measurement results of soybeans germinated with water, 0.1N NaCl, and 3N NaCl solutions, respectively.

Fig. 2 is a chromatogram showing the results of soy glycitin measurements germinated in water, 0.1N NaCl, and 3N NaCl solutions, respectively.

Figure 3 is a chromatogram showing the results of soybean genistin measurements germinated in water, 0.1N NaCl, and 3N NaCl solutions, respectively.

The results of measurement of soybean germination obtained in the above examples are shown in Table 1, and the content of isoflavones before germination as a comparative example is also shown.

Referring to FIGS. 1 to 3 and Table 1, the soybeans subjected to the germination process according to an embodiment of the present invention contain isoflavones of genistin, daidzin, and glycitin, .

In particular, the contents of genistin, daidzin, and glycitin, which are glycosides, were significantly increased, and the contents of non-glycosides genistein, daidzein, and glycitein (Glycitein) was also increased. The extract beverage according to one embodiment of the present invention has a high content of isoflavones and is effective in improving the female menopausal period and the premenstrual syndrome, and is expected to be effective in improving the anti-cancer effect and the vascular disease.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the present invention. It is evident that many variations are possible by those skilled in the art. It will be apparent to those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. something to do.

Claims (6)

Dipping soybeans in water for 10 to 14 hours and germinating at a temperature of 30 to 35 DEG C for 18 to 24 hours;
Wet-milling the germinated soybeans;
Separating the wet pulverized phosphorus and filtrate to obtain a filtrate; And
Preparing an extract beverage by the filtrate;
The method of manufacturing a soybean germinated and extracted beverage according to claim 1,
The method according to claim 1,
Wherein the soybean is germinated by immersing the soybean in 0.1 N to 3N NaCl solution in the germinating step.
The method according to claim 1,
Wherein the step of separating the soybean sprout and the filtrate further comprises adding water to the first separated soybean sponge, and further mixing and separating the filtrate.
The method according to claim 1,
Wherein the germinating step is performed while the black cloth is covered on the immersed soybeans.
The method according to claim 1,
Wherein the step of preparing the extracted beverage comprises mixing the physiologically active substance with the filtrate.
A soybean germinated extract beverage produced by the method of any one of claims 1 to 5.

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