KR20160019765A - Cosmetic or pharmaceutical composition for melanism, elasticity, anti-wrinkle, or skin moisturizing comprising dihydroartemisinin - Google Patents

Abstract

Provided in the present invention are: a composition comprising a compound represented by chemical formula 1 as an active ingredient for improving the elasticity of the skin, reducing wrinkles, moisturizing the skin, or blackening the skin or the hair; particularly a cosmetic composition; a pharmaceutical composition; and a health food product. The cosmetic or pharmaceutical composition of the present invention is safe to the human body with reduced side effects, and has excellent effects in blackening the skin and the hair, improving the elasticity of the skin, reducing wrinkles, moisturizing the skin, etc.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic or pharmaceutical composition for blackening, elasticity, wrinkle improvement, or moisturizing, including dihydroartemisinin,

The present invention relates to a cosmetic composition, a pharmaceutical composition and a health food, which have excellent effects such as blackening, elasticity, wrinkle improvement, moisturizing and the like, particularly excellent effects related to skin or hair. The present invention also relates to a cosmetic composition, a pharmaceutical composition and a health food which can exert such effects even in a small amount.

Due to the economic growth, the interest in the appearance as well as the health is increasing, and the desire to keep skin and keep healthy is also increasing. In recent years, not only healthy skin maintenance but also self-tanning products have been increasingly used to make skin look healthier. Self-tanning can avoid photo-aging due to overexposure to ultraviolet light, and it is possible to produce a tanning effect even in a season when sunlight does not shine. Recently used self-tanning products have been combined with keratin in the stratum corneum, such as Erythrulose and Dihydroxyacetone, to temporarily dye the skin and dye it. However, in the case of dihydroxyacetone, the skin tone is often not uniform according to the usage method, and there is a problem that the skin is smudged due to peeling of the keratin, and the erythrulose is colored with a more uniform skin tone, . In addition, it has been reported that exposure to ultraviolet light within 24 hours after application to the skin promotes active oxygen production of the skin.

In addition, gray hair generally occurs when the age is higher, giving the feeling of old age, and gray hair, especially at a young age, causes stress. White moths are more prominent in Oriental populations with black hair than white ones with bright hair color, and are being studied in health and beauty and psychology. Melanin is divided into red-yellow Pheomelanin and dark brown Eumelanin. Melanin in hair is an important factor that determines the color of hair. Melanin produced in the melanocyte present in the hair is hair After being transferred to the cortical cell keratinocytes, the hair moves upward with the growth of the hair. The gray hair, recognized as a phenomenon of physiological aging, is caused by a decrease in the number of hair melanocytes and a decrease in the production of melanin due to a decrease in function of melanocytes. Dyeing is currently used as a solution to white moth, but dyeing must be re-pigmented by growth of gray hair in a temporary manner, and the ingredients contained in the dye of hair dyes become irritants and cause contact dermatitis or skin allergy It is becoming a problem.

Collagen is a major substrate protein produced in the fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical durability of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, cell division and differentiation (Induction of growth of an organism or wound healing) are known. Such collagen is reduced by photoaging by age and ultraviolet irradiation, and collagenase activity, which degrades collagen, promotes collagen reduction. This is known to be closely related to the formation of wrinkles in the skin.

In addition, Elastin fiber forms crosslinks with collagen and is a skin constituent important for wrinkle formation involved in skin elasticity. The deficiency and aggregation of elastin fibers and the marked increase in the activity of the elastin degrading enzyme Elastase have been found to be one of the causes of skin wrinkles. It is known that Elastase is the only enzyme capable of degrading elastin, and its inhibition can fundamentally reduce the wrinkles of the skin.

On the other hand, collagen and elastin fibers are substrate proteins that play an important role in water retention in the dermal layer. These protein proteins adsorb moisture and increase the water holding capacity inside the structure, thereby allowing the skin to maintain proper moisture content. And it is known to be involved in maintaining the elasticity of the skin.

Presently, retinoids, adenosine, animal placenta-derived proteins, chlorella extract, and the like are known to improve wrinkles. The best known retinol promotes collagen synthesis and inhibits elastase enzymes, but it is unstable. When applied to the skin, it has a limited use due to safety problems such as irritation and redness, and chlorella extract has little effect, It is known that it is difficult to expect a wrinkle-improving effect.

Glycation generally refers to a reaction in which a simple sugar such as glucose or fructose forms a covalent bond to a protein or fat that occurs without involvement of the enzyme. The glycation takes place through a series of chemical reactions known as amarylidation, maillard reaction, resulting in the formation of advanced glycation endproducts (AGEs).

On the other hand, the glycation process of the protein does not involve the enzymatic action, so it occurs slowly, and as a result, the protein having a longer half-life such as collagen has more influence. Analysis of the final glycation products revealed that the more aged the glycated collagen was accumulated, the accumulation of the final glycated product changed the protein to a harder and more brittle state, and the glycated collagen was found in the extracellular matrix of the dermal layer (Dyer, DG et al., The Journal of Clinical Investigation., 9, p. 2463, 1993), which inhibits skin elasticity and promotes wrinkle formation by preventing collagen from forming a proper structure

Aminoguanidine, pyridoxamine, and aspirin are known to inhibit glycation. However, these substances are limited in their use due to the safety of the skin, There is a problem that can not be expected.

Accordingly, it is possible to provide a composition which is safe and biologically safe, has an excellent effect on skin elasticity, wrinkle, moisturizing effect, skin elasticity, wrinkle improvement, moisturizing effect or blackening effect of skin or hair, The development of a component having the above properties is urgently required.

Accordingly, the present invention provides such a useful use of a new active ingredient, i.e., an active ingredient, which solves the above problems and is safe for the human body and has excellent skin elasticity, wrinkle, moisturizing, or blackening of skin or hair, .

In other words, a problem to be solved by the present invention is to provide a composition comprising the active ingredient having excellent efficacy as an active ingredient.

In order to solve the above problems, the present invention relates to a composition for skin elasticity enhancement, wrinkle improvement, moisturizing, and blackening of skin or hair comprising dihydroartemisinin as an active ingredient, , A pharmaceutical composition or a health food.

That is, the present invention provides a novel use of dihydroartemisinin for skin elasticity enhancement, wrinkle improvement, moisturizing, and blackening of skin or hair.

The inventors of the present invention have found that dihydroartemisinin inhibits collagenase and elastase activity of skin and exhibits antagonism effect, thereby promoting regeneration of skin, moisturizing, skin elasticity and wrinkle-reducing effect And completed the present invention. Further, it has been confirmed that the dihydroartemisinin promotes the synthesis of melanin to show the blackening effect of the skin or hair, and the present invention has been completed.

In the present invention, 'blackening' means that the coloring matter of the skin or hair is increased, and in particular, the amount of melanin pigment is increased, which means that the skin or hair becomes dark color.

In the present invention, the term 'improving elasticity or skin elasticity' means reducing the degree to which the skin is stuck or stretched. In addition, the elasticity means that elasticity of the skin is maintained in a state where elastin and collagen are sufficiently present.

In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.

In the present invention, the term 'moisturizing or skin moisturizing' means increasing the skin moisture and maintaining a moist state. Skin moisturizing effect can help to improve wrinkles and elasticity of skin.

In order to solve the above-mentioned problems and to achieve the object of the present invention, the present invention provides a composition comprising a compound represented by the following general formula (1).

[Chemical Formula 1]

The compound represented by the formula (1) has the molecular formula C ₁₅ H ₂₄ O ₅ , the molecular weight is 284.35, and is named dihydroartemisinin, dihydroqinghaosu, artenimol or DHA.

The present invention is not particularly limited to the method for obtaining dihydroartemisinin, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

In the cosmetic composition, the pharmaceutical composition and the health food according to the present invention, the content of dihydroartemisinin is preferably 0.00001 to 10% by weight based on the total weight of the cosmetic composition, the pharmaceutical composition and the health food.

The dihydroartemisinin of the present invention may exist in solvated as well as unsolvated forms, including forms such as hydrates, ethanolates, and the like. The dihydroartemisinin of the present invention may exist in crystalline or amorphous form, and all such physical forms are included within the scope of the present invention.

The cosmetic composition for skin elasticity, wrinkle improvement, moisturizing effect, or blackening of skin or hair according to the present invention can be used as a cosmetic composition for skin, hair or the like in the form of a solution, an external ointment, a cream, a foam, a nutrition lotion, a softening water, The compositions of the present invention may be formulated as liquid cleansers, bath salts, sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant- containing cleansing oils, powder foundations, emulsion foundations, waxes, But are not limited thereto. ≪ Desc / Clms Page number 12 >

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier incorporated in a cosmetic composition for general skin, and examples thereof include oil, water, a surfactant, a moisturizer, a lower alcohol, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations. When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, -Butyl glycol oil, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiesters, fatty acid protein hydrolizates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like are used as carrier components .

The present invention also provides a method for skin elasticity, wrinkle improvement, moisturizing, or blackening of skin or hair, comprising the step of applying the compound represented by formula (1) to the individual's skin. The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

According to another embodiment of the present invention, there is provided a pharmaceutical composition for skin elasticity, wrinkle improvement, moisturizing, or blackening of skin or hair containing the compound of Chemical Formula 1 as an active ingredient.

The composition comprising the compound of the present invention may be various oral or parenteral formulations, but may preferably be a parenteral agent. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, and emulsions. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate.

The present invention can also be provided as a form of external skin preparation for skin elasticity, wrinkle improvement, moisturizing effect or blackening effect of skin or hair containing the compound of formula (1) as an active ingredient.

When the compound of formula (1) is used as an external preparation for skin, it may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, , Water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin And any other ingredients used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the compound of Formula 1 is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

The pharmaceutical composition of the present invention is particularly preferably used as a parenteral preparation. For example, the external preparation for skin may be a pharmaceutically acceptable base such as vaseline or stearyl alcohol; Suitable pharmaceutically acceptable surfactants such as polysorbate, sorbitan sesquioleate, and the like; A suitable pharmaceutically acceptable humectant such as glycerin; A suitable pharmaceutically acceptable solvent; And a conventional external preparation for skin preparation in which a flavoring agent, a coloring agent, a stabilizer, a tackifier and the like are homogeneously mixed.

When the compound of Chemical Formula 1 of the present invention is used as a medicine, it may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin elasticity, wrinkle improvement, moisturizing effect or blackening component of skin or hair. The skin elasticity, wrinkle improvement, moisturizing effect, or blackening effect of the skin or hair of the composition of the present invention can be further enhanced by the addition of the additional skin elasticity, wrinkle improvement, moisturizing or blackening component of skin or hair. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the invention, the composition is a skin elasticity or wrinkle improving ingredient known in the art as retinoic acid, TGF, animal placenta-derived proteins, betulinic acid and chlorella extract; As components for blackening known in the art, such as Erythrulose, Dihydroxyacetone; And derivatives thereof, and various plant extracts. ≪ Desc / Clms Page number 2 > The additional component may be included in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, ease of formulation of the compound of Formula 1 .

The pharmaceutical composition of the present invention can provide a desired skin elasticity, wrinkle, moisturizing effect, or blackening effect of skin or hair when an effective amount of the compound of Formula 1 is included. In the present invention, the term "effective amount" means an amount of a compound which exhibits a blackening effect of skin or hair, exhibits an elasticity improving effect and a wrinkle-improving effect, or exhibits a moisturizing effect.

An effective amount of the compound of formula (1) contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical product, the compound of Formula 1 may be contained at a higher concentration than that of a cosmetic product that is routinely applied to skin. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the compound of formula (1) 6 times a day.

According to another embodiment of the present invention, there is provided a skin elasticity improving effect, a wrinkle improving effect, a moisturizing effect, or a health food for skin or hair blackening effect containing the compound of formula (1).

The term 'health food' as used herein means a food prepared by adding the compound of formula (1) to food materials such as beverage, tea, spice, gum and confectionery, or by encapsulation, powdering or suspension, But it has the advantage that there are no side effects that can occur when a drug is used for a long period of time using a food as a raw material. Since the health food of the present invention thus obtained can be routinely ingested, it is very useful because it can be expected to have a high skin or hair blackening effect, an elasticity increasing effect, a wrinkle reducing effect, and a moisturizing effect.

When the compound of Chemical Formula 1 is used as a food additive, the compound of Chemical Formula 1 may be added as it is or may be used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range . There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense. The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention. In addition to the above, the health food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, , A carbonating agent used in carbonated drinks, and the like. In addition, the health food of the present invention may contain flesh for the production of natural fruit juice, fruit juice drink and vegetable drink. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In one embodiment of the present invention, the composition may include 0.00001 wt% to 10 wt% of the compound of Formula 1 based on the weight of the entire composition.

The composition containing the dihydroartemisinin of the present invention exhibits the blackening effect of the skin or hair.

The composition containing dihydroartemisinin of the present invention exhibits skin elasticity enhancement or wrinkle improvement effect.

The composition containing dihydroartemisinin of the present invention shows skin moisturizing effect.

The composition containing the dihydroartemisinin of the present invention can be used as safe and excellent cosmetic ingredients and pharmaceutical ingredients.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided by way of example to facilitate a specific understanding of the present invention.

Reference Example  One: Dihydroartemisinin ( Dihydroartemicinin ) Material information

[Chemical Formula 1]

Material name: Dihydroartemisinin

CAS No. : 71939-50-9

Molecular formula: C ₁₅ H ₂₄ O ₅

Molecular weight: 284.35

Where to buy: Tauto Biotech (China)

Example  One: Collagenase  Activity inhibitory effect

The inhibitory effect of collagenase, an enzyme that degrades collagen, was confirmed as follows.

Collagenase used collagenase derived from rat's interest and N- (3- [2-Furyl] acryloyl) -Leu-Gly-Pro-Ala was used as a collagenase substrate. The buffer solution used was a solution of 50 mM Tricine, 10 mM CaCl ₂ .H ₂ O, 400 mM NaCl (pH 7.5) dissolved therein. The synthetic substrate was dissolved in a buffer solution at a concentration of 10 mM and finally diluted to 75 μM. The collagenase was dissolved at a concentration of 1 unit / ml using cold third distilled water at 4 ° C and then diluted with buffer solution to a final concentration of 0.05 unit / ml. Collagenase inhibition candidates were tested at final concentrations of 25 and 50 ppm. At this time, the positive control group was set to have a final concentration of 50 ppm of epigallocatechin gallate (EGCG) whose collagenase inhibitory effect was known. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 10 minutes. Absorbance was measured at 345 nm using a spectrophotometer at intervals of 1 minute, and the maximum slope of absorbance versus time was determined to determine the activity of the enzyme. Collagenase inhibition rates were calculated as follows.

Inhibition of Collagenase Activity of Compound (1) (Number of Repeats = 3)

sample Enzyme activity Inhibition rate (%) The compound of formula (1) (50 ppm) -0.2 71.43 The compound of formula (1) (25 ppm) -0.5 28.57 The positive control (EGCG, 50 ppm) -0.6 14.29 The control (DMSO, 50 ppm) -0.7 -

As can be seen from the results shown in Table 1, the compound of Chemical Formula 1 is more excellent in inhibiting collagenase activity than EGCG, which is a positive control. That is, the compound of formula (1) exerts excellent collagenase activity inhibitory effect. Therefore, it was found that the compound of Chemical Formula 1 can be used for skin regeneration and wrinkle improvement.

Example  2: Elastase  Active inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastase was diluted with buffer solution to make a final concentration of 0.5 mM by making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an ELASTIC inhibitor. Ella stase inhibition candidates were added to give a final concentration of 10, 20 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

Inhibitory effect of the compound of formula (1) on elastase activity (number of repeats = 3)

sample Enzyme activity Inhibition rate (%) The compound of formula (1) (20 ppm) 5.4 48.08 The compound of formula (1) (10 ppm) 9.3 31.58 The positive control (Quercetin, 10 ppm) 3.5 66.35 The control (DMSO, 20 ppm) 10.4 -

As can be seen from the results in Table 2, when the compound of Chemical Formula 1 was treated, it was found that the effect was less than that of the positive control, but the inhibition of the elastase activity was excellent. That is, the compound of formula (1) exerted a superior inhibitory effect on elastase activity. Therefore, it was found that the compound of Chemical Formula 1 can be used for skin regeneration and wrinkle improvement.

Example  3: Blackening  Effect - Increase in the total amount of melanin

The compound of Chemical Formula 1 was added to the culture solution of mouse B-16 mouse melanoma cells to determine the blackening effect by measuring the total amount of melanin at the cellular level (Lotan R., Lotan D. Cancer Res . 40: 3345-3350, 1980). To evaluate the toxicity of melanoma cells in rats before the experiment and to determine the concentration without toxicity, blackening evaluation was performed.

The compound of formula (1) was added to the culture medium to a final concentration of 1 ppm and 10 ppm, and the control group, Forskolin, was added to the medium so as to be treated with B-16 melanoma cells and cultured for 3 days.

Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance at 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as an increase rate (%). The results are summarized in Table 3.

Increased effect of total melanin level at cell level according to concentration

sample Melanin production
(abs) Growth rate (%) Control group (DMSO, 10 ppm) 0.33 - The positive control (Forskolin, 10 ppm) 0.792 140 The compound of formula (1) (10 ppm) 0.396 20 The compound of formula (1) (1 ppm) 0.370 12.12

As can be seen from the results of Table 3, it was confirmed that the compound of Chemical Formula 1 increased the amount of melanin production as compared with that of the control, and that the production of melanin increased with increasing concentration.

Therefore, it was found that the compound of formula (1) can be used for blackening purposes.

Example  4: Antialcification  effect

In order to confirm the anti-glycation effect, the saccharification inhibitory activity was measured using L-arginine and glucose.

First, dissolve 1 M L-arginine and 1 M glucose in 1 M phosphate buffer (pH 7.4) and dilute the sample to 50 and 100 ppm using 1M phosphate buffer solution. Mix 1 M L-arginine and 1 M phosphate buffer at a ratio of 1: 4, and dispense 80 μl each into 96-well plates. Add 100 μl of 0.01 M aminoguanidine to be used as a positive control and a sample diluted to 50 and 100 ppm, respectively. Mix these samples well and then add glucose diluted in 1 M phosphate buffer to a final concentration of 0.1 M glucose. And reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation in the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. To measure the absorbance of the sample itself, absorbance was measured at 420 nm by adding 1M L-arginine and sample without glucose Respectively. The saccharide inhibitory activity can be obtained by the following formula.

The antagonism effect of the compound of formula (1)

sample Inhibition rate (%) The control (DMSO, 50 ppm) - The positive control (Aminoguanidine, 55 ppm) 54.8 The compound of formula (1) (50 ppm) 41.7 The compound of formula (1) (25 ppm) 25.6

As can be seen from the results of Table 4, the compound of Chemical Formula (1) shows excellent antihyperglycosylation effect and can have skin elasticity and wrinkle improving effect.

Claims (5)

1. A composition for skin or hair blackening comprising a compound represented by the following formula (1) as an active ingredient.
[Chemical Formula 1]

A composition for improving skin elasticity or wrinkle comprising a compound represented by the following formula (1) as an active ingredient.
[Chemical Formula 1]

A composition for moisturizing the skin comprising, as an active ingredient, a compound represented by the following formula (1).
[Chemical Formula 1]

4. The composition according to any one of claims 1 to 3, wherein the composition is used as a cosmetic composition, a pharmaceutical composition or a health food. The composition according to any one of claims 1 to 3, wherein the compound represented by Formula 1 is contained in an amount of 0.00001 to 10 wt% based on the total weight of the composition.