KR20150145947A - Cosmetic composition for ultraviolet-proof containing the extract of Uncaria tomentosa, Cetraria islandica and Euterpe oleracea - Google Patents

Abstract

The present invention relates to a cosmetic composition having an ultraviolet screening effect comprising a natural complex extract of Catskloof, Iceland moss and Asaiberi, and more specifically, to a cosmetic composition having an extract of Catskloof, Iceland moss and Asaiberry complex, And to a technique for producing a cosmetic composition having an effect of protecting skin from ultraviolet rays by alleviating irritation such as whitening, shine, and skin inflammation that can be caused by UV rays. The natural complex extract according to the present invention has an excellent antioxidative effect, is effective in inhibiting the expression of an inflammatory mediator, has an effect of regulating sebaceous secretion and protecting skin cells from ultraviolet rays as well as skin irritation alleviating effect, Cosmetics show excellent effect on ultraviolet ray shielding.

Description

[0001] The present invention relates to a cosmetic composition for ultraviolet screening comprising an extract of Catskloof, Iceland moss, and Asaiberry as an active ingredient,

The present invention relates to a cosmetic for protecting ultraviolet rays, and more specifically to a cosmetic composition for protecting ultraviolet rays, which comprises Cat's Clove Extract, Iceland Moss Extract and Asai Berry Extract, which is excellent in safety against skin, mitigates inflammatory reaction caused by ultraviolet rays, To a technique for producing an ultraviolet-blocking cosmetic composition.

Excessive exposure to ultraviolet light has many deleterious effects on our skin, including skin cancer, premature aging of skin, allergies, skin irritation, stinging and scaling. Therefore, sunscreen that protects skin from ultraviolet rays becomes important, and sunscreen products are increasing. The ultraviolet shielding index of currently commercially available ultraviolet screening products is gradually increasing and problems are also caused by this. By containing a large amount of ultraviolet ray blocking component, skin irritation such as erythema and inflammation may occur, and feeling of use and quality may be lowered due to opacity caused by ultraviolet blocking components, shine, and the like. Therefore, researches to develop new materials to alleviate these problems are under way.

Korean Patent Laid-Open Publication No. 2008-0103283 entitled "Ultraviolet barrier cosmetic composition" discloses an ultraviolet barrier cosmetic composition containing an ultraviolet barrier component comprising at least one selected from the group consisting of vanilla extract and chicory root extract. Korean Patent No. 0992088 entitled " Cosmetic Composition Containing Natural Plant Extract Providing Ultraviolet Radiation Blocking Effect "includes " Fermented Smelly Fruit (seeds of Lotus), Gaza (Fruit of Gramineae), and Alpinia officinarum Hance ) And a natural plant extract obtained by low-temperature ultrasonic extraction of Abies koreana. The present invention also provides a natural plant extract which provides an ultraviolet blocking effect.

The present inventors have completed the present invention by confirming that they have excellent UV blocking activity in a mixed extract of Catskloof, Iceland moss and Asaiberry while continuing their research on natural materials having a skin-safe and excellent ultraviolet shielding effect.

It is an object of the present invention to provide a cosmetic composition for ultraviolet ray shielding containing a natural complex extract of Catskloof, Icelandic moss and Asai Berry.

Another object of the present invention is to provide a method for producing a natural combination extract of Catskloof, Iceland moss, and Asaiberi, which is effective for antioxidative effects, skin irritation mitigation, suppression of sebum secretion, and ultraviolet light shielding.

In order to achieve the above object, there is provided a cosmetic composition for protecting against ultraviolet rays, which comprises a natural combination extract of Catskloof, Iceland moss, and Asai berry. The natural complex extract is obtained by mixing Catskloof, Iceland moss and Acai Berry. At this time, the natural complex extract of Catskloof, Iceland moss and Asaiberi as an active ingredient is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The natural combination extract of Catskloof, Iceland moss and Asai berry is a hot-water extract or a mixture of at least one selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol Solvent extract by solvent. More preferably, it is a hot-water extract.

Preferably, the extracts of Catskloof, Iceland moss and Asaiberry natural complex are extracted by mixing Catskloof, Icelandic moss and Acaiberi at a weight ratio of 1: 2: 1 to 2: 1: 2, Are mixed in a ratio of 2: 1: 2.

According to another aspect of the present invention, there is provided a method for preparing a cucumber seedlings comprising: (A) mixing a catsclover, an iced moss and an assyberry at a weight ratio of 1: 2: 1 to 2: 1 to 2; (B) extracting the mixture with water, at least one solvent selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol; And (C) sterilizing, followed by filtration and concentration.

The natural extracts of Catskloof, Iceland moss and Asaiberi according to the present invention exhibit excellent antioxidative, skin irritation, suppression of sebum secretion and ultraviolet ray blocking effect.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising a complex extract of Catskloof, Icelandic moss and Asaibera, and having antioxidation, skin irritation mitigation, sebum secretion inhibiting effect and excellent ultraviolet shielding effect.

Uncaria tomentosa is native to the Amazon River basin, mainly in the Andes of Peru, a Latin American country, and is also native to Latin America. Catskloof has been used as a special medicine to alleviate aching pain such as arthralgia and back pain since ancient Inca Empire period. By eating cat claws, immunity normalizes, inflammation and pain are suppressed, blood flow improves and blood flow improves. Cats claws not only have a UV protection effect, but also have effects such as anti-inflammation, anti-bacterial, cell protection, immunity enhancement, anti-aging, pore contraction and so on to protect skin from ultraviolet rays and relieve stimulation from ultraviolet rays.

Icelandic lichens ( Cetraria islandica ), a kind of lichen, have been used in many countries for centuries as a treatment for two disorders, tuberculosis, and health promoters, and the European Health Agency has also licensed it as a treatment for sore throat and respiratory disorders. These polysaccharides extracted from mosses are known to have anti-inflammatory and immunity-enhancing effects. It is also known that Iceland moss induces anti-inflammatory effects by inducing cytokine secretion changes (IL-12, IL-10).

Easter pest ( Euterpe leracea ) is a fruit harvested from tropical palm trees in the northernmost Amazon region of Brazil, the last clean area of the earth, containing a large amount of nutrients. Aboriginal peoples of the Amazon have used it for the purpose of treating and strengthening the disease, and it is called "Amazon Viagra" or "Amazon's wine" for its superior efficacy. Researchers at Brunswick Laboratories, a USDA research organization, have shown that antioxidants are the most effective antioxidants on the planet. The antioxidant activity of Asai berry is up to 33 times higher than that of red grapes and 6 times higher than that of blueberries.

According to the present invention, there is provided a cosmetic composition for protecting against ultraviolet rays, which comprises a natural complex extract of Catskloof, Icelandic moss and Asai berry.

At this time, the natural combination extract of Catskloof, Iceland moss, and Asaiberi is a hot-water extract or a mixture of water and a lower alcohol selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol At least one solvent. The complex extract may be an extract obtained by mixing Catskloof, Iceland moss and Asai Berry, or may be used in the form of an extract obtained by extracting each with the solvent.

According to one embodiment of the present invention, the complex extract may be prepared by the following method. After drying, the cut-off cats, Iceland moss, and Asai berries are mixed at a weight ratio of 1: 2: 1 to 2: 1 to 2. 5 to 25 times the weight of the solvent is added to the mixture. The solvent may be selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether At least one solvent may be used. Then, it is sterilized, filtered and concentrated to prepare a natural complex extract of Catskloof, Iceland moss, and Asaibery.

In the cosmetic composition of the present invention, the above-mentioned natural complex extract of Catskloof, Iceland moss and Asaiberry is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

The thus-produced Catskloof, Iceland moss, and Asaiberi natural complex extracts showed antioxidation (Test Example 1), inhibition of the expression of inflammation-related factors (Test Example 2) , And suppression of 5 alpha-reductase expression (Test Example 3). In addition, it showed excellent effects in protecting cells from ultraviolet rays (Test Example 4), ultraviolet blocking (Test Example 5), and skin irritation mitigation (Test Example 6). The cosmetic composition containing the natural complex extract of Catskloof, Iceland moss and Asaiberi according to the present invention can be prepared into any of the commonly-used formulations, and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, Body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, make-up base and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Example 1: Production of a natural complex of Catskloof, Iceland moss and Asaiberry

After drying, we added 3 kg of 70% hydrous ethanol to a sample of 50 g of Catskloof, 50 g of Iceland moss and 50 g of Asaiberry (dry weight ratio = 1: 1: 1) And extracted with boiling time. After the extraction, the mixture was filtered through a 300-mesh filter paper, left at room temperature for 3 days, and the precipitate was filtered twice using a filter paper of Edbentek No. 5 and a Waterman GFC 47 mm filter. The mixture was concentrated at 50 ° C using a vacuum concentrator and then dried using a spray dryer (manufactured by BUCHI) to obtain a mixed extract powder having a dry weight of 21.7 g.

Examples 2 to 7: Preparation of natural complex extract of Catskloof, Iceland moss and Asaiberry

As shown in the following Table 1, the mixed extract powder was prepared by extracting and filtering in the same manner as in Example 1, except that the mixing weight ratio of Catskloof, Iceland moss and Acai Berry was varied.

(Weight ratio) Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Cat claw One One One 2 2 2 Icelandic moss One 2 2 One One 2 Asai Berry 2 One 2 One 2 One

Comparative Example 1: Production of cat claw extract powder

The extracts were boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser, filtered through a 300-mesh filter paper, left at room temperature for 3 days, and the precipitate was applied to Edbentec 5 And filtered twice with Watts GFC 47 mm filter paper. Then, the mixture was concentrated at 50 ° C using a vacuum concentrator, and then dried using a spray dryer (manufactured by BUCHI) to obtain a mixed extract powder having a dry weight of 20.9 g.

Comparative Example 2: Preparation of Iceland moss extract powder

The extracts were boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser, filtered through a 300-mesh filter paper, left at room temperature for 3 days, and the precipitate was applied to Edbentec 5 And filtered twice with Watts GFC 47 mm filter paper. The mixture was concentrated at 50 ° C using a vacuum condenser, and then dried using a spray dryer (manufactured by BUCHI) to obtain a mixed extract powder having a dry weight of 15.8 g.

Comparative Example 3: Preparation of Asaiberi extract powder

After extracting and drying, 1 kg of 70% aqueous ethanol was added to 50 g of Asai berry, which was thoroughly boiled for 8 hours at 100 ° C. in an extractor equipped with a condenser. The mixture was filtered through a 300-mesh filter paper and left at room temperature for 3 days. And filtered twice with Watts GFC 47 mm filter paper. Then, the mixture was concentrated at 50 ° C using a vacuum condenser, and then dried using a spray dryer (manufactured by BUCHI) to obtain a mixed extract powder having a dry weight of 20.2 g.

Examples 8 to 14: Preparation of formulations using Catskloof, Iceland moss and Asaiberry natural complex extracts

Cream containing Catskloof, Iceland moss, and Asaiberi natural complex extract prepared in the above Examples was prepared according to a conventional method with the composition and content shown in Table 2 below.

(weight%)
Control Example Comparative Example Example 4 5 6 8 9 10 11 12 13 14 Cat's Clove Extract (Comparative Example 1) - 0.5 - - - - - - - - - Icelandic moss extract
(Comparative Example 2) - - 0.5 - - - - - - - - Asaiberry extract (Comparative Example 3) - - - 0.5 - - - - - - - Natural combination extract (Example 1) - - - - 0.5 - - - - - - Natural combination extract (Example 2) - - - - - 0.5 - - - - - Natural combination extract (Example 3) - - - - - - 0.5 - - - - Natural combination extract (Example 4) - - - - - - - 0.5 - - - Natural combination extract (Example 5) - - - - - - - - 0.5 - - The natural complex extract (Example 6) - - - - - - - - - 0.5 - The natural complex extract (Example 7) - - - - - - - - - - 0.5 Ethylenediamine acetic acid,
This sodium salt 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Guar hydroxypropyltrimonium
Chloride 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 glycerin 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Panthenol 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Cocamidopropyl betaine 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Ammonium lauryl sulfate 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Laura Maid Diai 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0  Glycollosteralate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sodium lauryl sulfate 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Sodium lauryl sulfate (30%) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 Chloride set remnium 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Methylchloroisothiazolinone
(1.05-1.2%) 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Methyl isothiazolinone
(0.3 to 0.45%) 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Magnesium chloride (0.5 to 1.0%) 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Magnesium nitrate (21.0 to 23.5%) 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Citric acid 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 Dimethicone (55 to 60%) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Laureth -3 (2-4%) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Laureth-20 (2-4%) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 incense 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

Test Example 1: Measurement of antioxidative effect using NBT method

To confirm the antioxidative effects of the extracts of Catskloof, Icelandic moss and Asaiberry obtained in Examples 1 to 7, antioxidative activity was measured using NBT method using other antioxidants, that is, BHT, as a comparative sample under laboratory conditions Respectively. In order to measure the antioxidative effect, the active oxygen produced by xanthine and xanthine oxidase was measured by the NBT method and the effect of the test substance on the removal of active oxygen, that is, the effect of active oxygen scavenging was evaluated. The active oxygen generated by xanthine and xanthine oxidase was reacted with Nitro Blue Tetrazolium (NBT), and the blue color produced thereby was measured at a wavelength of 560 nm to measure the active oxygen scavenging rate. The method of measuring the active oxygen scavenging rate is as follows. 0.1 ml of a 3 mM EDTA solution, 0.1 ml of a BSA solution and 0.1 ml of a 0.72 mM NBT solution were added to a vial (5 ml vial), to which 0.1 ml of 0.1 wt% wt of DMSO (dimethyl sulfoxide) / v)% concentration is added to the sample solution, and the solution is allowed to stand at 25 DEG C for 10 minutes. Thereafter, 0.1 ml of xanthine oxidase solution is added, and the mixture is stirred rapidly and incubation is started at 25 ° C for 20 minutes. Then, 0.1 ml of a 6 mM CuCl ₂ solution is added to stop the reaction, and the absorbance St at 560 nm is measured. The blank test is carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt is measured. Again, the blank of the sample solution is measured by the same procedure using distilled water instead of the xanthine oxidase solution to determine the absorbance Bo. The results of the effects were calculated by the following formula, and the results are shown in Table 3 below.

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] 100

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

Name of sample Treatment concentration
(w / v%) Antioxidative effect
(%) Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 1) 0.1 90 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 2) 0.1 91 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 3) 0.1 93 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 4) 0.1 92 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 5) 0.1 92 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 6) 0.1 96 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 7) 0.1 93 Cat's Clove Extract (Comparative Example 1) 0.1 84 Iceland moss extract (Comparative Example 2) 0.1 83 Asaiberry extract (Comparative Example 3) 0.1 89 BHT (butylated hydroxyanisole, DUKSAN KOREA) 0.1 90

As shown in Table 3, the antioxidative effect was confirmed in all of the samples tested at a concentration of 0.1 (wt / v)%. Especially, the natural extracts of Catskloof, Icelandic moss and Asaiberry showed superior antioxidative effects and superior antioxidative effects to BHT, compared to Catskloof, Icelandic moss or Asaiberry extract alone. Among them, the best antioxidative effect was shown in Example 6.

Test Example 2: Confirmation of suppression effect of inflammatory mediator expression by ultraviolet irradiation

RAW 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After incubation, starvation was maintained for 12 hours, LPS 10 ng / mL and samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of TNF-α expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The inhibition rate of TNF-α expression was calculated by the following equation, and the results are shown in Table 4 below. Dexamethasone was used as a control.

TNF-α expression level (%) = [(TNF-α expression level-sample TNF-α expression level) / control TNF-α expression level] × 100

Name of sample Treatment concentration
(w / v%) TNF-a
% Inhibition Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 1) 0.1 84 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 2) 0.1 85 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 3) 0.1 84 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 4) 0.1 87 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 5) 0.1 85 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 6) 0.1 92 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 7) 0.1 84 Cat's Clove Extract (Comparative Example 1) 0.1 81 Iceland moss extract (Comparative Example 2) 0.1 83 Asaiberry extract (Comparative Example 3) 0.1 80 Dexamethason (Sigma) 0.1 88

As shown in Table 4, TNF-α inhibitory effect was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the extracts of Catskloof, Iceland moss and Asaiberi showed a superior TNF-α inhibitory effect compared with the extracts of Catskloof, Iceland moss or Asaiberry alone, and showed the most excellent TNF-α inhibitory effect in Example 6 .

Test Example 3: Confirmation of 5 alpha-reductase inhibition

HEK cells were adjusted to 1 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, inoculated on a 6-well plate, and cultured for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of 5α-reductase expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). 5α-reductase expression inhibition rate was calculated by the following equation, and the results are shown in Table 5 below. Dexamethasone was used as a control.

5α-reductase expression level (%) = [(5α-reductase expression level - sample 5α-reductase expression level) / control 5α-reductase expression level] × 100

Name of sample Treatment concentration
(w / v%) 5α-reductase
% Inhibition Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 1) 0.1 83 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 2) 0.1 86 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 3) 0.1 85 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 4) 0.1 84 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 5) 0.1 86 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 6) 0.1 92 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 7) 0.1 87 Cat's Clove Extract (Comparative Example 1) 0.1 83 Iceland moss extract (Comparative Example 2) 0.1 82 Asaiberry extract (Comparative Example 3) 0.1 80 Dexamethason (Sigma) 0.1 88

As shown in Table 5, the inhibitory effect of 5α-reductase was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the extracts of Catskloof, Icelandic moss and Asaiberi showed a superior 5α-reductase inhibitory effect compared to the extracts of Catskloof, Icelandic moss or Asaiberry alone.

Test Example 4: Determination of cell viability by ultraviolet irradiation

Human-derived fibroblasts were cultured in 96-well plates at a concentration of 2 × 10 ⁵ cells / well, and 0.2 ml of IMDM medium containing 10% FBS was added and cultured in a CO ₂ incubator for 24 hours. After the sample solution dissolved in DMSO was treated, the amount of ultraviolet light of 2 MED was irradiated for 10 minutes and cultured for 24 hours. Then, MTT assay was performed to determine cell survival rate. In MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. Then, the medium was removed, and 0.5 ml of DMSO was added, and the formed pomace cup was measured for absorbance at 570 nm using ELISA. BHT was used as a control.

Name of sample Treatment concentration
(w / v%) cell
Survival rate (%) Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 1) 0.1 85 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 2) 0.1 87 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 3) 0.1 84 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 4) 0.1 86 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 5) 0.1 85 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 6) 0.1 93 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 7) 0.1 88 Cat's Clove Extract (Comparative Example 1) 0.1 82 Iceland moss extract (Comparative Example 2) 0.1 83 Asaiberry extract (Comparative Example 3) 0.1 81 BHT (butylated hydroxyanisole, DUKSAN KOREA) 0.1 82

As can be seen in Table 6, the cell viability was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the extracts of Catskloof, Icelandic moss and Asaiberry complex showed excellent cell survival rates even after irradiation with ultraviolet light, as compared with extracts of Catskloof, Iceland moss or Asaiberry alone.

Test Example 5: Confirmation of ultraviolet blocking effect

Catskloof, Iceland moss, and Acai Berry natural combination extracts. Experiments were carried out using an in-vitro method. The instrument used for the measurement was SPF 290 (Optometrics Corporation, UK). The results are shown in Table 7 below.

Name of sample SPF value UVA / UVB ratio Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 8) 23.7 0.42 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 9) 24.4 0.44 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 10) 22.5 0.43 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 11) 23.8 0.41 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 12) 23.5 0.48 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 13) 25.5 0.38 Catskloof, Icelandic Moss and Assayberry Natural Composite Extract
(Example 14) 22.3 0.42 Cat's Clove Extract (Comparative Example 4) 18.2 0.50 Iceland moss extract (Comparative Example 5) 15.5 0.55 Asaiberi extract (Comparative Example 6) 20.0 0.52

As can be seen in Table 7 above, the cream compositions of Examples 8-14 containing the natural combination extract of Catskloof, Iceland moss and Asaiuberi of the present invention were mixed more than when containing Catskloof, Iceland moss or Asaiberry alone Showed excellent UV blocking effect.

Test Example 6: Confirmation of skin irritation mitigation effect

      In 10 or 20 year old male and female subjects without skin allergy symptoms, the inside of the lower arm was irradiated with ultraviolet rays to induce skin irritation, and then the cosmetic composition containing the natural complex extract according to the present invention was applied The relative degree of sedation of the induced stimulus was evaluated relative. First, two parts of the lower part of the lower arm were irradiated with ultraviolet rays to cause skin erythema. After the erythema was induced, the cosmetic composition containing the natural complex extract according to the present invention was applied and the cosmetic composition containing no natural complex extract according to the present invention was applied. The degree of skin soothing was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Catskloof, Icelandic moss and Asaiberi natural complex extract (Example 8) One One Catskloof, Icelandic moss and Asaiberry natural combination extract (Example 9) One One Catskloof, Icelandic moss and Asaiberi natural complex extract (Example 10) One One Catskloof, Icelandic moss, and Asaiberry complex (Example 11) One One Catskloof, Icelandic moss, and Asaiberry complex (Example 12) One 0 Catskloof, Icelandic moss, and Asaiberry complex (Example 13) 0 0 Catskloof, Icelandic moss, and Asaiberry complex (Example 14) 0 One Cat's Clove Extract (Comparative Example 4) One One Iceland moss extract (Comparative Example 5) One 2 Asaiberi extract (Comparative Example 6) One One

As can be seen in Table 8 above, the cream compositions of Examples 8-14 containing Catskloof, Icelandic moss and Asaiberi natural combination extracts were superior to Catskloof, Iceland moss or Asaiberry when mixed alone, Skin irritation mitigation effect. Among them, the cream formulation of Example 13 showed the best skin irritation mitigation effect.

Claims (10)

Catskloof, Iceland moss, and Asaiberry natural combination extract. [Claim 2] The method according to claim 1, wherein the natural complex extract of Catskloof, Iceland moss and Asaiberry is mixed with Catskloof, Icelandic moss and Asaiberry at a weight ratio of 1: 2: 1 to 2: Wherein the cosmetic composition is an ultraviolet ray blocking cosmetic composition. [Claim 3] The cosmetic composition for protecting against UV rays according to claim 2, wherein the cosmetic composition is obtained by mixing the Catskloof, Iceland moss, and Asaiberry at a weight ratio of 2: 1: 2, respectively. The cosmetic composition for ultraviolet screening according to claim 1, wherein the natural complex extract is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition has an antioxidative effect. The cosmetic composition for protecting against UV rays according to claim 1, wherein the cosmetic composition inhibits inflammation of the skin by inhibiting the expression of an inflammatory mediator of skin due to ultraviolet rays. The cosmetic composition according to claim 1, wherein the cosmetic composition controls sebaceous secretion through inhibition of 5 alpha -reductase. The cosmetic composition for protecting ultraviolet rays according to claim 1, wherein the cosmetic composition has an effect of protecting cells from ultraviolet rays. The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of alleviating skin irritation from ultraviolet rays. (A) mixing cat claws, Iceland moss, and acai berry at a weight ratio of 1: 2: 1 to 2: 1 to 2;
(B) extracting the mixture with water, at least one solvent selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol; And
(C) sterilizing, and then filtering and concentrating the natural complex extract of Catskloof, Iceland moss and Asaiberry.