KR20150106478A - Extract of Black raspberry containing lactic acid bacterium for immunological enhancement and improving climacteric symptoms - Google Patents

Abstract

The present invention provides a composition for improving immunity and improving menopausal symptoms comprising bacterium oil and lactic acid bacteria as an active ingredient. The complex composition comprising the bacterium oil and the lactic acid bacterium according to the present invention not only increases the intestinal beneficial bacteria but also provides the effect of enhancing the immune system and improving the symptoms of menopausal symptoms. In addition, since it uses bokbunja oil extracted from bokbunjae which is an abandoned agricultural product byproduct, it is safe as a food material and has an economical advantage in manufacturing.

Description

{Extract of black raspberry containing lactic acid bacterium for immunological enhancement and improving climacteric symptoms}

The present invention relates to a composition for improving immunity and improving menopausal symptoms comprising bacterium oil and lactic acid bacteria as an active ingredient.

Black raspberry is the fruit of Rubus coreanus Miq., A perennial deciduous shrub belonging to Rosaceae. China is known as a country of origin and Jeju and southern regions are known to be the major producing regions in Korea. Bokbunja is harvested in early summer and used for food. It has been known as a valuable medicinal product for protecting tannins, gonjeongje, and liver in the past, and it is a practical material for physical weakness, , YS, Chang, HG, J. Korean Soc. Agr. Chem. Biotechnol. 46: 367-375, 2003; Heo, J., Dongyeubogam. Publications on oreiental medicine written]. Among the components contained in the bokbunja, the stem contains flavan 3-ol (-) - epicatechin ((-) - epicatechin), (+) - catechin 4 (procyanidin B-4). Leaves are known to contain kaempferol, quercetin, quercetin 3-O-β-D-glucuronoide ), Ellagic acid, and acid globulin H-5 (sanguin H-5). In addition, the presence of quercetin and various phenol compounds from bokbunja fruit has been reported [Yoon, I., Cho, JY, JH, Wee, JH, Jang, MY, Ahn, TH, Park, Food Sci. Technol., 34: 898-904, 2002). In addition, various phenolic compounds are known to exist in the immature stage, and gallotannin, ellagitannin, gallic acid, and 2,3- (S) -HHDP-D-glue The presence of caprylanoside, acid H-4 and H-6 was confirmed (Pang, GC, Kom, MS, Lee, MW, Korean J. Pharmacogn. 27: 366-370, 1996].

The present inventors have conducted continuous studies on bokbunja, and found that bokbunja oil extracted from bokbunja seed, which is not a hydrothermal extract of bokbunja fruit generally used for food, has a high content of omega-3, omega-6, ellagitannins, Stearic acid, oleic acid, and the like, and thus it can be used as a functional ingredient (Korean Patent Registration No. 1168169). Continuously, the present inventors have studied various physiological activity regulating functions of brambled oil, and as a result, they found immune enhancement of brambled oil and improving activity of menopausal symptoms and completed the present invention.

The present invention is to provide a composite composition comprising bacterium oil and lactic acid bacteria, which can be used for improving immunity and improving menopausal symptoms, as an active ingredient.

The present inventors have studied various physiological activity control functions of brambly oil and have sought to develop a composite material that can be used for improving immunity or improving menopausal symptoms. As a result, the present inventors have completed the present invention by confirming that the complex composition of bokbunja oil and lactic acid bacterium has excellent immunity enhancing effect.

The present invention provides a composition for improving immunity and improving menopausal symptoms comprising bacterium oil and lactic acid bacteria.

In the present invention, the term " immune enhancement " refers to a generally referred to as immunity enhancement. More specifically, it is a concept including activation of macrophages, complement, etc. of the human body and induction of expression of various immune related cytokines.

On the other hand, climacteric (climacteric) is a type of endocrine syndrome, which means that the physiological function and sexual function are reduced or lost due to hormonal changes. In women, menopause, a permanent process of menstruation that occurs after cessation of ovarian function, is a process of menopause. Postmenopausal women may have acute chronic symptoms due to changes in hormones, such as decreased estrogen production, follicular stimulating hormone (FSH), and elevated luteininzing hormone (LH). Early symptoms of female menopausal symptoms may include psychological symptoms such as vasomotor symptoms such as hot flush and night sweat, anxiety, loss of concentration, depression, Urogenital and epidermal manifestations that occur within a few years may be osteoporosis, cardiovascular and cerebrovascular disease after years of menopause. Men also experience menopausal symptoms due to changes in secretion of testosterone. Testosterone is thought to affect male genital development, skeletal or muscular development, sexual arousal, agitation, and hyperactivity in the brain and mental faculties. It is known that serum testosterone decreases its concentration when subjected to strong stress. When serum testosterone is lowered, it causes diseases such as male menopausal disorder or pubertal disease (遲发 症). Men's menopausal disorders can result in a variety of symptoms (for example, depression, depression, anxiety, anxiety, nervousness, loss of vigor, fatigue, arthritis, myositis, muscle weakness, sweating, facial flushing, sleep disturbances, Loss of appetite, loss of libido, erectile dysfunction, loss of appetite) are strongly expressed. In the present invention, improvement of menopausal symptoms includes improvement of such female menopausal symptoms or male menopausal symptoms.

In one embodiment of the present invention, the bacterium oil and the lactic acid bacterium exhibit a much improved immune enhancing effect as compared with the immunopotentiating effect of the conventionally known lactic acid bacteria, and have the effect of improving the menopausal symptoms of women and men as well . Therefore, a complex composition of bacterium oil and lactic acid bacteria can be used as an active ingredient of a composition for improving immunity and improving menopausal symptoms.

In the present invention, the bramble oil includes all of the oil components of the bramble seeds produced by a known method.

Known methods for producing bokromatic oil include a cold pressing method, a hot pressing method, a low temperature pressing method, and a solvent extraction method.

In the present invention, the cold pressing method is a method of obtaining oil by directly pressing the seed which is flowing when heat is applied without heat treatment, whereby coloring matter, direction and gum quality are discharged into the oil when heat is applied, The hot pressing method is a method of pressing at a high temperature of 100 to 250 DEG C and then pressing it. The low temperature pressing method is a method of pressing at 43 DEG C Or less, and squeeze the oil. The solvent extraction method is a method of extracting with a solvent such as n-hexane.

For example, the present invention is able to obtain bramble oil by milking brambly seeds preferably at 43 ° C to 200 ° C for 60 to 120 minutes, more preferably at 120 to 190 ° C for 60 to 120 minutes .

In the present invention, the solvent extraction method refers to a method of producing an extract from bramblymus seeds using water, an organic solvent, or a mixture thereof as an extraction solvent. The kind of the organic solvent used at this time and the mixing ratio of water and the organic solvent are not particularly limited.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, as the lower alcohol, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol can be used. The organic solvent may be a polar solvent such as acetic acid, dimethyl-formamide (DMFO) or dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, decane 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1- chloropropane, chlorobenzene , Non-polar solvents such as benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (tetrahydrofuran) may be used.

In one embodiment, the brambly oil may be obtained by extracting brambly seed using hexane.

The bramble oil used in the present specification includes not only the crude extract obtained from the bramble seeds by compression or solvent extraction but also the processed product thereof.

On the other hand, lactic acid bacteria are known to have an immunostimulating effect due to a peptidoglycan component present in a cell wall. Therefore, the inventors of the present invention have conducted studies on the effect of selecting lactic acid bacteria as a candidate substance having immunity enhancing effect that can be used in combination with bokbunja oil and concurrently administering them. As a result, unexpectedly, The activity of improving immunity and / or improving menopausal symptoms in the co-administration group was significantly higher than that in the control group.

In the present invention, the lactic acid bacterium to be used in combination with the bacterium oil may be a live bacterium or a bacterium. In one embodiment, the lactic acid bacteria that may be used in the present invention may be quaternary. The dead bacteria also contain a large amount of the peptidoglycan component, and when dead cells are used, it is not necessary to carry out a step such as a coating for improving the survival rate until intestinal transit, so that a large amount of lactic acid bacteria can be effectively delivered into the intestines Can be used.

In the present invention, lactic acid bacteria include components derived from a lactic acid bacterium or a lactic acid bacterium obtained from a culture, and processed products such as lyophilized products thereof.

In one embodiment, the lactic acid bacteria of the invention are selected from the group consisting of Lactobacillus sp . ), Spololactobacillus sp. ( Sporolactobacilus sp . ), The genus Streptococcus sp . ), The genus Lactococcus sp . ), Luconostoc genus ( Leuconostoc sp . ), The genus Pedioococcus ( Pedioococcus sp . ), Enterococcus ( Enterococcus sp . ) And Bifidobacterium sp . ). ≪ / RTI >

Meanwhile, the composition for improving immunity and improving menopausal symptoms, which comprises the bacterium oil and the lactic acid bacterium according to the present invention, may be used as a pharmaceutical composition or a food composition.

As used herein, the term " comprising as an active ingredient " is meant to include an amount sufficient to achieve efficacy or activity of the botulinum and / or lactic acid bacteria.

In one embodiment of the invention, the bacteriological oil and / or lactic acid bacteria in the composition of the present invention may be administered at a dose of, for example, 0.001 mg / kg or more, preferably 0.1 mg / kg or more, more preferably 10 mg / More preferably at least 100 mg / kg, even more preferably at least 250 mg / kg, most preferably at least 0.1 g / kg.

The bacterium oil and the lactic acid bacterium are ingredients that are allowed as foods, and even when administered in an excess amount, there is no adverse effect on the human body, so that the quantitative upper limit of these components contained in the composition of the present invention can be selected by a person skilled in the art within a suitable range.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions and the like. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention can be administered orally. The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Usually, a skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g / kg.

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The present invention also provides a food composition for improving immunity and improving menopausal symptoms comprising bacterium oil and lactic acid bacteria as an active ingredient.

The food composition according to the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a health functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectioneries, diet bars, dairy products, meat, chocolates, pizza, ram noodles, other noodles, gums, ice cream, .

The food composition of the present invention may contain, as active ingredients, bacterium oils and lactic acid bacteria, as well as components that are ordinarily added during the manufacture of foods, including, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavors do. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, it may further include citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, various plant extracts and the like in addition to the bokbunja oil and lactic acid bacteria of the present invention have.

The present invention provides a health functional food containing the bacterium oil and the lactic acid bacterium as an active ingredient and a food composition for improving immunity and improving menopausal symptoms. The health functional food is a food prepared by adding the active ingredient of the present invention to food materials such as beverage, tea, spice, gum and confectionery, or by encapsulation, powdering, suspension or the like, However, unlike general medicine, there is an advantage that there is no side effect that can occur when a food is used as a raw material for a long period of taking the medicine. The health functional food of the present invention thus obtained is very useful because it can be ingested routinely. The addition amount of the bacterium oil and the lactic acid bacterium in such a health functional food can not be uniformly determined depending on the kind of the health functional food to which it is added but may be added within a range that does not impair the original taste of the food, And usually ranges from 0.01 to 50% by weight, preferably from 0.1 to 20% by weight. In the case of a health functional food in the form of a liquid, a pill, a granule, a tablet or a capsule, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a liquid, a capsule, or a drink.

The present invention also provides the use of a complex composition comprising the bacterium oil and the lactic acid bacterium for the manufacture of a medicament or food for improving immunity and improving menopausal symptoms. As described above, the complex composition comprising the bacterium oil and the lactic acid bacterium can be used for the purpose of improving immunity and improving the symptoms of menopausal symptoms.

The present invention also provides a method for improving immune enhancement or menopausal symptoms comprising administering to a mammal an effective amount of a complex composition comprising a bacterium oil and a lactic acid bacterium.

The term "mammal " as used herein refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term "effective amount" refers to the amount of active ingredient or pharmaceutical composition that elicits a biological or medical response in a tissue system, animal, or human, as contemplated by a researcher, veterinarian, physician or other clinician, ≪ / RTI > inducing a reduction of the symptoms of the disease or disorder. It will be apparent to those skilled in the art that the effective amount and the administration frequency of the active ingredient of the present invention will vary depending on the desired effect. Thus, the optimal dosage to be administered can be readily determined by those skilled in the art and will vary with the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, The age, body weight, sex, diet, time of administration, route of administration and fraction of the composition, duration of treatment, concurrent medication, and the like. In the prevention, treatment, or improvement of the present invention, in the case of an adult, it is preferable to administer the composition of the present invention at a dose of 0.001 mg / kg to 10 g / kg once to several times a day.

The complex composition comprising the bacterium oil and the lactic acid bacterium according to the present invention not only increases the intestinal beneficial bacteria but also provides the effect of enhancing the immune system and improving the symptoms of menopausal symptoms. In addition, since it uses bokbunja oil extracted from bokbunjae which is an abandoned agricultural product byproduct, it is safe as a food material and has an economical advantage in manufacturing.

[ Example ]

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

≪ Example 1 >

Bokbunja fruit grown in Gochang area, Jeollabuk-do ( Rubus coreanum fruit) was added to 1 kg of water and the juice was firstly squeezed at room temperature for 2 hours to obtain 120 g of juice. The residue after the first pouring was put into a vacuum press and compressed to obtain an additional 40 g of a brambly concentrate.

The residue after the first extraction and the residue after the compression were collected and concentrated under reduced pressure using a vacuum rotary condenser to remove solvent components and then hot air dried to separate the bacterium seeds. The bacterium seeds were removed by shaking in a 0.1 mm sieve, And centrifuged at 3000 rpm for 20 minutes to isolate only the brambled seed (70 g).

70 g of the obtained bramble seeds were placed in a milking machine and subjected to a pressure-tight milking method to obtain a bramble oil (12 g).

Claims (12)

Wherein the composition comprises bacterium oil and lactic acid bacterium as an active ingredient.
The method according to claim 1,
Wherein the brambled oil is obtained by a pressing method from brambly seeds.
The method according to claim 1,
Wherein the bramble oil is a hexane extract of bramble seeds.
The method according to claim 1,
Wherein the Lactobacillus acidophilus is a deadly bacterium.
The method according to claim 1,
The lactic acid bacteria may be selected from the group consisting of Lactobacillus sp . ), Spololactobacillus sp. ( Sporolactobacilus sp . ), The genus Streptococcus sp . ), The genus Lactococcus sp . ), Luconostoc genus ( Leuconostoc sp . ), The genus Pedioococcus ( Pedioococcus sp . ), Enterococcus ( Enterococcus sp . ) And Bifidobacterium sp . ). ≪ / RTI >
Wherein the composition contains baclofen oil and lactic acid bacterium as an active ingredient.
The method according to claim 6,
Wherein the brambled oil is obtained by a pressing method from brambles seeds.
The method according to claim 6,
Wherein the bramble oil is a hexane extract of bramble seeds.
The method according to claim 6,
A composition for improving the symptoms of menopausal symptoms, wherein the Lactobacillus acidophilus is a deadly bacterium.
The method according to claim 6,
The lactic acid bacteria may be selected from the group consisting of Lactobacillus sp . ), Spololactobacillus sp. ( Sporolactobacilus sp . ), The genus Streptococcus sp . ), The genus Lactococcus sp . ), Luconostoc genus ( Leuconostoc sp . ), The genus Pedioococcus ( Pedioococcus sp . ), Enterococcus ( Enterococcus sp . ) And Bifidobacterium sp . ). ≪ / RTI > The method according to claim 6,
Wherein the food composition is for improving female menopausal symptoms.
The method according to claim 6,
Wherein the food composition is for the improvement of male menopausal symptoms.