KR20150057333A - Composition comprising extract of korean fir for preventing and improving obesity - Google Patents

Abstract

The present invention relates to a composition containing a coniferous tree extract as an active ingredient. Such a composition can be used for preventing or ameliorating obesity and hyperlipemia which can be caused by excessive blood lipid by controlling blood lipid levels by inhibiting lipase activity. Thus, it can be administered to a patient having obesity or hyperlipemia as a pharmaceutical composition, and can also be taken as a food composition to improve, prevent or treat the disease in terms of diet.

Description

Technical Field [0001] The present invention relates to a composition for prevention or improvement of obesity,

The present invention relates to a composition for preventing or ameliorating obesity hyperlipidemia comprising a coniferous tree extract, and more particularly, to a composition for preventing or ameliorating obesity hyperlipidemia comprising a coniferous tree extract, wherein the composition inhibits lipase activity, For example, obesity, hyperlipidemia, and cardiovascular disease.

Obesity refers to a state in which energy intake and consumption are not balanced by genetic causes or lifestyle factors, excess energy is accumulated in fat, resulting in abnormally increased body fat and metabolic abnormalities. Obesity is a serious health problem in Korea as well as in western countries, which is a meat eating stock. It is a major cause of increased risk of hypertension, hyperlipidemia, atherosclerosis, heart disease and diabetes as well as psychological and social inconvenience. About 30% to 40% of modern people are known to have obesity, and in advanced countries, 2% to 7% of the total national medical costs are caused by overweight and obesity. In Korea, the socio-economic costs of obesity spent in and out of the health care system have increased to about 1.17 billion won in 1998 and about 1.8 billion won in 2005. If the current rate of obesity is maintained, It is expected to increase continuously.

The treatment and prevention of obesity has been actively studied in various countries, and it has been studied in diverse ways, including dietary therapy (dietary restriction, low-energy therapy, ultra low-energy therapy, fasting therapy) , Exercise therapy that emits energy through exercise, psychotherapy (behavior modification therapy, cognitive behavior therapy), drug metabolism promotion drug, appetite suppressant, drug therapy using digestion absorption inhibitor, Surgery, etc. are currently known.

However, there is a need for a method that can safely and steadily manage body weight at an appropriate level in everyday life rather than improvement by surgical therapy or drug. Therefore, there is a need to develop new natural materials that are effective in reducing body fat and effective in weight reduction. Therefore, the present inventors have studied the possibility of application as a food from a plant which has not been widely known.

KR10-2011-0068958A

Thus, the present inventors have found that the extract of coniferous tree can inhibit the activity of lipase which decomposes fatty acids and improve the blood lipid level. Thus, the present invention has been completed.

Accordingly, an object of the present invention is to provide a composition capable of preventing and / or ameliorating obesity and hyperlipidemia, which is capable of inhibiting the activity of lipase and controlling the blood lipid level by incorporating a coniferous tree extract as an active ingredient.

        The food composition according to the present invention is characterized by containing a coniferous tree extract.

        The composition comprising the coniferous tree extract according to the present invention inhibits the activity of lipase and prevents or improves safe and effective obesity, hyperlipidemia, which can control blood lipid levels. Accordingly, the composition according to the present invention can be used as a composition capable of preventing or ameliorating obesity and hyperlipemia, which can be caused by excessive blood lipids by controlling blood lipid levels by inhibiting lipase activity very safely and effectively.

In one aspect, the present disclosure may relate to a composition comprising an Abies Koreana extract as an active ingredient.

In one aspect of the present disclosure, the composition may be a composition for preventing, ameliorating, or treating obesity or hyperlipidemia.

In one aspect of the present disclosure, the composition may be a pharmaceutical, food, or cosmetic composition.

In one aspect of the present specification, the coniferous tree may be at least one selected from the group consisting of leaves, bark, and cones of conifer trees.

In one aspect of the present invention, the coniferous tree extract may be a mixture of leaves, cones and bark in a weight ratio of 7: 2: 1. The weight ratio may be a weight ratio of 1 to 10: 1 to 3: 1, or a weight ratio of 6 to 8: 1.5 to 2.5: 1.

In one aspect of the present disclosure, the coniferous tree extract may be one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. The organic solvent may be one or more selected from the group consisting of ethanol, butylene glycol, and propylene glycol.

In one aspect of the present disclosure, the composition may be characterized as inhibiting lipase activity.

The composition for external application for skin according to the present invention can be used for the purpose of improving various metabolic diseases caused by lipid excess in blood, specifically, symptoms such as obesity and hyperlipemia, and further for the treatment and prevention of these diseases. To this end, the composition of the present invention may be provided as a pharmaceutical composition for the purpose of treatment and prevention of the disease, and may be provided as a functional food composition for the purpose of improving symptoms of the disease.

In this specification, the Korean fir ( Abies Koreana ) is an evergreen coniferous tree of the pine tree, and is a native plant that grows in Korea at 500-2,000m above sea level in Mt. Halla, Mt. Jiri, Mt. It is used as building material and furniture, and it is planted in parks, botanical gardens, gardens and so on. The coniferous trees used in the present invention were sampled from landscape water plants at Amorepacific Research Institute.

The method for producing the coniferous tree extract used in the present specification is not particularly limited, and in the present specification, the coniferous tree extract is a concentrate obtained by leaching from the coniferous tree, transferring the extract, concentrating the leached solution partially or completely, An extract obtained by drying water, or a chemical substance itself exhibiting the main effect contained in the extract.

As used herein, the coniferous tree may be contained in the form of an extract, or may be contained as a pulverized product of the herbal medicine itself, or as a dried pulverized product of the herbal medicine, but is not limited thereto. In addition, there is no limitation to the method of obtaining the coniferous tree used in the present invention. The coniferous tree used in the present invention is not limited and may be cultivated and used or purchased from the market, and some or all of the ground or roots of the tree can be used. Can be used. Specifically, at least one selected from the group consisting of leaves, bark, and cones of conifer wood can be used.

As used herein, the term " extract "includes all substances obtained by extracting components of a natural product, regardless of the extraction method, extraction solvent, extracted component or extract form, It is a broad concept that includes all of the materials that can be obtained by processing or processing in a process.

In the present specification, " coniferous tree extract " includes all of the substances obtained by extracting the components of the coniferous tree, regardless of the extraction method, the extraction solvent, the extracted components or the form of the extract. In the process of extracting the components, (eg, acid, base, enzymes, etc.) and also extracts the components of the coniferous tree and extracts the extracted material, which can then be processed or processed in other ways. It is a broad concept that encompasses all of the substances in question.

In one aspect of the present disclosure, the coniferous tree extract comprises washing and drying the coniferous tree; Solvent extraction of the dried coniferous tree; And filtering out the extracted spherical tree. The solvent includes at least one selected from the group consisting of water, an organic solvent, and a mixture of water and an organic solvent. Wherein the water comprises distilled or purified water and the organic solvent is selected from the group consisting of alcohols such as C ₁ -C ₅ lower alcohols, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone and chloroform But is not limited thereto.

The coniferous tree extract may contain a conifer-C ₁ -C ₆ alcohol extract, and specifically the alcohol may be methanol or ethanol.

As used herein, the coniferous tree extract may be a crude extract of a solvent selected from the group consisting of water, C ₁ -C ₆ alcohols, and combinations thereof. The C ₁ -C ₆ alcohol may specifically be methanol. When extracting the coniferous tree with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times of the coniferous tree. Specifically, the extraction is preferably performed by adding about 10 times the solvent, but the present invention is not limited thereto. The extraction may be performed by heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or the like, and there is no limitation as long as it is an extraction method that is obvious to a person skilled in the art. The extraction may be carried out at room temperature, but it may be carried out under heating at a temperature of about 40 to 100 ° C, more preferably about 80 ° C, for a more efficient extraction, It is not. The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but it is not limited thereto and may be varied depending on conditions such as extraction solvent and extraction temperature. The extraction may be carried out one or more times several times to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times of continuous extraction.

In this specification, the coniferous tree extract may contain a crude extract of a coniferous tree as described above, and may be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having a low polarity. Examples of the organic solvent include, but are not limited to, hexane, methylene chloride, ethyl acetate, n-butanol, and the like. The extract or the soluble fraction of the extract may be used as it is, or may be used as an extract form by concentration after filtration, and may be used as a form of a lyophilizate by concentration and freeze-drying.

The coniferous tree extract of the present invention comprises a step of finely pulverizing and drying the coniferous tree; Adding water, an organic solvent, or a combination thereof to the dried spherical tree, followed by reflux extraction; Precipitating the extract and filtering with a filter cloth; Centrifuging the filtrate to separate the residue and the filtrate; And concentrating the separated filtrate under reduced pressure to obtain a spherical tree extract.

In one aspect of the present disclosure, the coniferous tree extract is characterized in that the conifer-concentrated tree concentrate according to the above method is composed of purified water, ethanol, butylene glycol, and propylene glycol so as to contain 5% by weight based on the total weight of the extract Lt; RTI ID = 0.0 > dispersing / dissolving < / RTI >

For example, water or an organic solvent may be added to finely and dried spherical trees in the process of extracting the spherical tree extract of the present invention, followed by reflux extraction and precipitation, followed by filtration and centrifugal separation of the residue and filtrate, The separated filtrate is concentrated under reduced pressure at 40 to 45 ° C to obtain a coniferous tree extract. The organic solvent which can be used in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of water and organic solvent. In consideration of the safety of raw materials, 100% water, 70% Or Butylene Glycol is used. At this time, the extraction temperature is preferably 18 to 80 ° C in the case of water, and preferably 18 to 40 ° C in the case of 70% ethanol and butylene glycol, and the extraction time is preferably 6 to 168 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed. After obtaining the extract using a solvent, the extract can be obtained by cooling, heating and filtering at room temperature by a conventional method known in the art, or the solvent can be further evaporated, spray dried or freeze-dried. In lyophilization, lyophilization can be performed at -50 to -70 ° C for 3 to 4 days.

The composition of the present invention preferably contains the spherical tree extract in an amount of 0.05 to 50% by weight based on the total weight of the composition. More preferably, 0.01 to 1% by weight or 0.5 to 10% by weight can be used. When the amount is less than 0.05% by weight, it is difficult to expect the efficacy thereof. Specifically, the coniferous tree extract may contain at least 0.0001% by weight, at least 0.005% by weight, at least 0.001% by weight, at least 0.01% by weight, at least 0.1% by weight, at least 0.3% by weight, at least 0.5% by weight, at least 0.8% by weight At least 1 wt%, at least 3 wt%, at least 5 wt%, at least 7 wt%, at least 10 wt%, at least 15 wt%, at least 20 wt%, at least 25 wt%, at least 30 wt% , Or 40 wt% or more, and 60 wt% or less, or 50 wt% or less.

Also in one aspect of the present disclosure, the coniferous tree extract may be included at a concentration of from 1 ppm to 1000 ppm based on the total volume of the composition. Specifically, the coniferous tree extract may contain at least 1 ppm, at least 10 ppm, at least 50 ppm, at least 100 ppm, at least 150 ppm, at least 200 ppm, at least 250 ppm, at least 300 ppm, at least 400 ppm, at least 450 ppm, at least 470 ppm, at least 490 ppm, at least 500 ppm, at least 510 ppm, at least 530 ppm , 550 ppm or more, 600 ppm or more, 650 ppm or more, 700 ppm or more, or 800 ppm or more, and 2000 ppm or less or 1000 ppm or less.

The pharmaceutical compositions according to the present disclosure may be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, etc. These solid preparations may contain one or more excipients such as starch, calcium carbonate, sucrose, Or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical dosage forms of the compositions herein may be used in the form of their pharmaceutically acceptable salts and may also be used alone or in combination with other pharmaceutically active compounds, as well as in a suitable set. The salt is not particularly limited as long as it is pharmaceutically acceptable so long as it is pharmaceutically acceptable and includes, for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, , Benzenesulfonic acid, toluenesulfonic acid, and naphthalenesulfonic acid.

The composition of the present invention may be administered parenterally or orally, and may be administered in one to several divided doses in an amount of 0.1 to 500 mg, preferably 1 to 100 mg per kg of body weight per day have. The dosage for a particular patient may vary depending on the patient's body weight, age, sex, health condition, diet, time of administration, administration method, excretion rate, severity of disease, and the like.

The pharmaceutical composition according to the present invention may be formulated into tablets or capsules by conventional methods such as powders, granules, tablets, soft or hard capsules, oral preparations such as suspensions, emulsions, syrups, , Injectable solutions, sterile injection solutions, and the like, and may be used in any form suitable for pharmaceutical preparations.

The compositions according to the present disclosure may be administered to mammals such as rats, mice, livestock, humans, and the like in a variety of routes including parenterally, orally, and all manner of administration may be expected, for example, Or by intravenous, intramuscular, subcutaneous, intramural or intracerebroventricular injection.

In one aspect of the present disclosure, the food composition may be a health functional food composition.

The formulation of the food composition according to the present specification is not particularly limited, but may be formulated into, for example, tablets, granules, powders, liquid preparations such as a drink, caramels, gels, bars and the like. The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients.

In the food composition according to the present specification, the dosage determination of the active ingredient is within the level of those skilled in the art, and its daily dosage is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, mg / kg / day to 500 mg / kg / day, but it is not limited thereto, and may vary depending on various factors such as the age, health condition, and complication of the subject.

The food composition according to the present invention may be used as a food or beverage such as various foods such as chewing gum, caramel product, candy, ice cream, confectionery, beverage such as soft drink, mineral water, alcoholic beverage, healthful food including vitamins and minerals .

In addition to the above, the food composition which is one aspect of the present invention may be in the form of a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, Alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks and the like. In addition, the functional food compositions of the present disclosure may include natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally comprised in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition herein.

The formulation of the cosmetic composition according to one aspect of the present specification is not particularly limited and can be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing But the present invention is not limited thereto, and may be manufactured by any one or more formulations selected from the group consisting of a foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

When the formulation of the cosmetic composition according to the present invention is a paste, a cream or a gel, the carrier component may be an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition according to the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, Propellants such as hydrocarbons, propane / butane or dimethyl ether.

Solvents, solubilizing agents or emulsifying agents are used as carrier components in the case of formulations of cosmetic compositions according to the present invention in the form of solutions or emulsions, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition according to the present disclosure is a suspension, it may contain, as carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

In the case of an interface-active agent-containing cleansing of the cosmetic composition according to the present invention, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition according to the present invention may further comprise ingredients included in functional additives and common cosmetic compositions in addition to the coniferous tree extract. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The cosmetic composition of the present specification may further comprise, in addition to the above-described functional additive, components contained in a general cosmetic composition as required. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

Hereinafter, the configurations and effects of the present invention will be described in more detail with reference to examples, test examples, and production examples. However, these examples, test examples, and production examples are provided for illustrative purposes only in order to facilitate understanding of the present specification, and the scope of the present specification is not limited by the following examples.

Manufacture of coniferous tree extract

A total of 1 kg of leaves, cones, and bark of shade tree, shade and bark were shredded three times for 24 hours in 70% ethanol aqueous solution at a weight ratio of 7: 2: 1, Filtered. The filtered extract was concentrated under reduced pressure at 45 ° C or less and freeze-dried at -60 ° C for 3 days.

The concentrate was dispersed / dissolved in ethanol so as to contain 5% by weight based on the total weight of the coniferous tree extract to prepare a coniferous tree extract.

[Test Example 1] Lipase activity inhibitory effect

Lipase inhibitory activity was assessed using previously reported methods (Nakai, N. et al. Inhibitory effects of oolong tea polyphenols on pancreatic lipase in vitro J. Agric. Food Chem. 53, 4593-4598 (2005). ). The enzyme solution was prepared by dissolving porcine pancreatic lipase (Sigma, St. Louis, MO) in 0.1 M Tris-HCl buffer (pH 8). The spherical tree extract of Example 1 was mixed with enzyme buffer to a concentration of 0.5 ug / mL, and 100 ul was allowed to stand at 37 캜 for 15 minutes. 10 μL of 10 mM para-nitrophenylbutyrate (p-NPB) was added, and the enzyme reaction was carried out at 37 ° C. for 15 minutes. Lipase activity was measured by measuring the hydrolysis of p-NPB to para-nitrophenol at 405 nm using a microplate reader.

The following equation was used for the calculation of the inhibition of activity.

Relative pancreatic lipase activity (%) =

 (Activity of compound in substrate - activity of negative control substance in the absence of substrate) / (activity of compound when substrate is present - activity of negative control substance when substrate and compound are absent) x 100.

As the negative control group, third distilled water was used. The inhibition rate of lipase activity was calculated according to the above formula (1), and the results are shown in Table 2.

Component (ppm) Example 1 Comparative Example 1 Conifer tree extract 500 -

Relative pancreatic lipase activity (%) Example 1 93.6 ± 8.4 Comparative Example 1 100.0 ± 3.2

As shown in Table 1, it was found that the spherical tree extract of the present invention has an effect of inhibiting lipase activity at a low concentration of 500 ppm, as compared with Comparative Example 1, which does not contain spherical tree extract.

Lipase is an enzyme that hydrolyzes fat to fatty acids and glycerol and is involved in the digestion of fatty acids. The inhibition of the lipase activity inhibits the digestion of the fat in the body and suppresses the absorption of the lipase. Therefore, it is possible to prevent and improve the diseases such as obesity and hyperlipemia, which are absorbed and accumulated in the body excessively, Effect. Therefore, the composition containing the extract of the coniferous tree according to the present invention has an effect of inhibiting the lipase activity as in the experimental example, and thus it can be confirmed that the composition has a preventive and improving effect on diseases such as obesity or hyperlipidemia.

Formulation examples of compositions according to one aspect of the present disclosure are described below, but are also applicable to various other formulations, which are intended to be illustrative only and not for purposes of limitation.

[Formulation Example 1] Soft capsule

The soft capsule filling liquid is prepared by mixing 8 mg of the coniferous tree extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of vegetable hydrogenated oil, 4 mg of yellow radish and 9 mg of lecithin and mixing them according to a conventional method. 400 mg per capsule is filled to prepare a soft capsule. Separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin and 10 parts by weight of sorbitol solution, and filled with the filling solution to prepare a soft capsule containing 400 mg of the composition according to the present invention.

[Formulation Example 2] Tablets

The mixture was mixed with 8 mg of the coniferous tree extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose, granulated using a fluidized bed drier, and 6 mg of sugar ester . 500 mg of these compositions are tabletted by conventional methods to prepare tablets.

[Formulation Example 3] Drinking agent

After mixing 8 mg of the extract of the sphincter of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid and 25 g of liquid oligosaccharide, 300 ml of purified water is added to fill each bottle 200 ml each. It is filled in a bottle and sterilized at 130 ° C for 4 to 5 seconds to prepare a drink.

[Formulation Example 4]

8 mg of the spherical tree extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch are mixed and granulated into a granule using a fluidized bed granulator.

[Formulation Example 5]

Injections were prepared in a conventional manner according to the composition shown in Table 3 below.

Compounding ingredient content The coniferous tree extract of Example 1 10-50 mg Sterile sterilized water for injection Suitable amount pH adjusting agent Suitable amount

[Formulation Example 6] Health functional food

Health functional foods were prepared according to the compositions shown in Table 4 below in a conventional manner.

Compounding ingredient content The coniferous tree extract of Example 1 20 mg Vitamin A acetate 70 μg Vitamin E 1.0 mg Vitamin B1 0.13 mg Vitamin B2 0.15 mg Vitamin B6 0.5 mg Vitamin B12 0.2 μg Vitamin C 10 mg Biotin 10 μg Nicotinic acid amide 1.7 mg Folic acid 50 μg Calcium pantothenate 0.5 mg Ferrous sulfate 1.75 mg Zinc oxide 0.82 mg Magnesium carbonate 25.3 mg Potassium monophosphate 15 mg Dicalcium phosphate 55 mg Potassium citrate 90 mg Calcium carbonate 100 mg Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a component suitable for a health functional food as a preferred embodiment, the compounding ratio may be arbitrarily modified.

[Formulation Example 7] Health drinks

Health drinks were prepared in a conventional manner according to the composition shown in Table 5 below.

Compounding ingredient content The coniferous tree extract of Example 1 1000 mg Citric acid 1000 mg oligosaccharide 100 g Taurine 1g Purified water Balance

The above components are mixed according to a conventional health drink manufacturing method, and the mixture is stirred and heated at 85 DEG C for about 1 hour, and then the solution is sterilized by filtration.

 [Formulation Example 8] Flexible longevity (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 6 below.

Compounding ingredient Content (% by weight) The coniferous tree extract of Example 1 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 9] Nutritional lotion (milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 7 below.

Compounding ingredient Content (% by weight) The coniferous tree extract of Example 1 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 10] Nourishing cream

Nutritive creams were prepared according to the compositions listed in Table 8 below in a conventional manner.

Compounding ingredient Content (% by weight) The coniferous tree extract of Example 1 2.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 11] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 9 below.

Compounding ingredient Content (% by weight) The coniferous tree extract of Example 1 2.0 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 12] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 10 below.

Compounding ingredient Content (% by weight) The coniferous tree extract of Example 1 0.2 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

Having described specific portions of the disclosure in detail, those skilled in the art will appreciate that these specific embodiments are merely exemplary and are not intended to limit the scope of the disclosure. Accordingly, the actual scope of the disclosure will be defined by the appended claims and their equivalents.

Claims (9)

A composition for preventing, improving or treating obesity or hyperlipidemia comprising an Abies Koreana extract as an active ingredient. The composition according to claim 1, wherein the coniferous tree is at least one selected from the group consisting of leaves, bark, and cones of conifer wood. The composition of claim 1, wherein the coniferous tree extract is at least one extract selected from the group consisting of water, organic solvents, and mixtures thereof. The composition according to claim 3, wherein the organic solvent is at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol. The composition according to claim 1, wherein the spherical herb extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. 6. The composition according to claim 5, wherein the coniferous tree extract is contained in an amount of 0.01 to 1% by weight based on the total weight of the composition. 2. The composition of claim 1, wherein the composition inhibits lipase activity. 8. The composition according to any one of claims 1 to 7, wherein the composition is a pharmaceutical or a food composition. 8. The composition according to any one of claims 1 to 7, wherein the composition is a formulation selected from the group consisting of a soft capsule, a tablet, a granule, and a drink.