KR20150056208A - A novel Lactobacillus fermentum SJ8256 and use thereof - Google Patents

Abstract

The present invention relates to novel Lactobacillus buffer momentum SJ8256 (Lactobacillus fermentum SJ8256; (KCTC 12516BP), a culture of the above-mentioned strain, or a mixture thereof as an active ingredient. More particularly, the present invention relates to a microbial preparation for controlling bacterial pathogen in an animal husbandry pathogen. More specifically, Lactobacillus fermentum SJ8256 isolated from nature is used as a salmonella When mixed with Salmonella sp. And E. coli , the Lactobacillus strain exhibited a strong antimicrobial effect against the above-mentioned multi-resistant pathogens, which was remarkably elevated as compared with various antibiotics currently used Therefore, the Lactobacillus perfumant SJ8256 strain, a culture solution thereof, or a mixture thereof can be usefully used as an effective ingredient of a microbial preparation for controlling an animal pathogenic bacterium.

Description

New Lactobacillus fermentum SJ8256 strain and its use {A novel Lactobacillus fermentum SJ8256 and use thereof}

The present invention relates to a novel Lactobacillus fermentum SJ8256 strain and a microorganism preparation using the strain.

Due to the rapid development of industry and economy, the national diet has been westernized, and as a result, the consumption of livestock products has increased, and in the livestock industry, not only the prevention and treatment of livestock, but also the improvement of livestock productivity by promoting livestock growth and improving feed efficiency The use of various antibiotics for the purpose of. As of 2000, about 12,000 tons of antibiotics were added to feed for the purpose of promoting livestock growth, and about 900 tons of antibiotics for the treatment of diseases were also reported.

However, as the antibiotics in livestock feeds have presented various possible side effects to the human body, consumer sentiment has also changed due to the consumption of antibiotics consumed, and nowadays, The use of antibiotics added to feed for the purpose of promoting growth is totally prohibited or regulated. Specifically, in Europe, the use of antibiotics added to feeds has been banned since January 2006, and Korea has banned the use of antibiotics as a growth promoter for livestock since July 2011.

Accordingly, as a new feed additive to which a safe and environmentally-friendly alternative substance capable of replacing the antibiotic has been required, attention has been focused on a probiotics, i.e., a microorganism exhibiting an antibacterial effect as probiotics. The probiotics include microorganisms that help balance intestinal microorganisms, microorganisms that exhibit antimicrobial activity and enzyme activities, and metabolites produced by the microorganisms, and are fed to humans or animals in the form of dried cells or fermented products , And live bacteria in the form of single or multiple strains showing the effect of improving intestinal blight in a person or a house.

Lactic acid bacteria (LAB) is known as an antibacterial probiotics through the production of various antibacterial substances such as lactic acid, acetic acid, and other organic acids or bacteriocin, and it is an important biological preservative of food or livestock feed It is known to be possible. In addition, it enhances the self-defense ability of plants by producing various physiologically active substances, antimicrobial substances and anticancer substances, and in the case of livestock, stabilizes intestinal microorganisms, increases feed efficiency and increases disease resistance. The scope of the current lactic acid-related microorganisms used agents include Bifidobacterium (Bifidus), Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Lactococcus (Lactococcus), Staphylococcus aureus (Staphylococcus) and a base Kono and the like stock (Leuconostoc), the lactic acid bacteria In order to properly use it industrially, it is necessary to judge the proper characteristics according to the use purpose.

As an example of the probiotics reported so far, Korean Patent Laid-Open Publication No. 10-2012-0118570 discloses a Lactococcus lactis ET 45 strain and the bacteriocin produced therefrom have been disclosed in Korean Patent Laid-Open Publication No. 10-89530, and lactic acid bacteria such as Lactobacillus plantarum ) SY99 strain and the bacteriocin produced therefrom. Korean Patent Laid-Open Publication No. 10-2007-0018138 discloses Lactobacillus fermentum IB261 ( Lactobacillus < RTI ID = 0.0 > fermentum IB261) strain KCTC 18107P, Korean Patent Laid-Open No. 10-2010-0076263 discloses a probiotic which is isolated from calf feces and prevents bowel disease in cattle, and Lactobacillus BLAB19 fermentum BLAB19). However, as a probiotic strain for industrial use, there is a continuing demand for the development of a strain capable of broadly replacing commonly used antibiotics and capable of simultaneous application to various strains or a natural substance produced therefrom.

Accordingly, the present inventors have made efforts to discover new microbial preparations for controlling livestock pathogenic microbes to replace antibiotics used in the past, while exhibiting antimicrobial effects against a variety of livestock pathogenic bacteria. As a result, Lactobacillus fermentum SJ8256 ( Lactobacillus fermentum SJ8256, Accession No .: KCTC 12516BP) was mixed with Salmonella sp. and E. coli , which are highly resistant pathogenic strains, the Lactobacillus strain has a strong antimicrobial effect against the above-mentioned multi-resistant pathogens And that this is a remarkably elevated effect when compared with various antibiotics currently used, thereby completing the present invention.

The object of the present invention is to provide a novel Lactobacillus fermentum SJ8256 ( Lactobacillus fermentum SJ8256).

It is another object of the present invention to provide a microbial preparation for replacing antibiotics used in the past while exhibiting an antimicrobial effect against various livestock pathogenic bacteria.

In order to achieve the above object, the present invention has been deposited with accession number of KCTC 12516BP Lactobacillus buffer momentum SJ8256 (Lactobacillus fermentum SJ8256).

In addition, the present invention provides a microorganism preparation containing the Lactobacillus perfumant SJ8256 strain, a culture thereof or a mixture thereof as an active ingredient.

In addition, the present invention provides a microbial preparation for controlling an animal pathogenic bacterium containing the Lactobacillus perfumant SJ8256 strain, a culture thereof or a mixture thereof as an active ingredient.

When mixed cultures of Salmonella sp. And E. coli , which are highly resistant pathogenic bacteria, Lactobacillus fermentum SJ8256 ( Lactobacillus fermentum SJ8256, Accession No .: KCTC 12516BP) isolated from nature of the present invention, Lactobacillus strain SJ8256 exhibits a potent antimicrobial effect against the above-mentioned multi-resistant pathogens and exhibits a remarkably elevated effect when compared with various antibiotics currently used. Therefore, the Lactobacillus perfumant SJ8256 strain is a microorganism preparation for controlling livestock pathogenic bacteria Can be usefully used as an active ingredient.

Figure 1 is a graph showing the effect of Lactobacillus fermentum SJ8256 ( Lactobacillus sp.) Against E. coli and Salmonella sp. fermentum SJ8256). < / RTI >

Hereinafter, the present invention will be described in detail.

The present invention provides a Lactobacillus fermentum SJ8256 strain deposited with accession number KCTC 12516BP.

In addition, the present invention provides a microbial preparation for controlling an animal pathogenic bacterium containing the Lactobacillus perfumant SJ8256 strain, a culture thereof or a mixture thereof as an active ingredient.

Preferably, the strain exhibits antagonism to either or both of Salmonella sp. And E. coli , but is not limited thereto.

Preferably, the strain, the culture or the mixture thereof is contained in an amount of 10 to 20 parts by weight, but is not limited thereto.

In a specific example of the present invention, in order to isolate an antimicrobial activity strain exhibiting an antimicrobial activity against an animal pathogenic bacterium, the present inventors isolated the Lactobacillus perfumant SJ8256 strain from nature and isolated the base of 16s rRNA described in SEQ ID NO: 1 Sequence, which is 99% or more homologous to the Lactobacillus perfumant species, the Lactobacillus perfumant SJ8256 was found to be a novel strain most closely related to the lactobacillus fermentum species strain ( 1), and deposited on Nov. 7, 2013 with the Korean Agency of Bioscience and Biotechnology (Accession No. KCTC 12516BP). In addition, it was confirmed that Lactobacillus perfumant SJ8256 has antibacterial activity against E. coli and Salmonella spp., Which inhibits the growth of pathogenic microorganisms (see FIG. 2).

Therefore, the lactobacillus fermentum SJ8256 strain of the present invention exhibits a strong antimicrobial effect against the above-mentioned multi-resistant pathogens when cultivated together with Escherichia coli and Salmonella spp., Which are highly resistant pathogens, and thus the Lactobacillus perfumant SJ8256 strain of the present invention , A culture solution thereof, or a mixture thereof can be usefully used as an effective ingredient of a microorganism preparation for controlling an animal pathogenic bacterium.

The microorganism preparation of the present invention can be produced according to a conventional method for producing a probiotic composition, and can be generally in the form of a culture suspension or a dry powder. In addition, one or two or more pharmaceutically acceptable conventional carriers or one or two or more additives may be selected from the above-mentioned lactobacillus fermentum SJ8256 strain as a main component or an effective amount of the culture solution thereof to prepare a composition of a conventional formulation can do.

The carrier may be selected from one or more selected from among diluents, lubricants, binders, disintegrants, sweeteners, stabilizers and preservatives. Examples of the additives include one or more of flavorings, vitamins and antioxidants Can be used.

In the present invention, the carrier and additives may be any pharmaceutically acceptable ones. Specific examples of the diluent include lactose monohydrate, trehalose, cornstarch, soybean oil, Microcrystalline cellulose or mannitol may be preferably used and the lubricant may be magnesium stearate or talc. The binder may be polyvinylpyrrolidone (PVP) or hydroxypropyl It is preferable to select from hydroxypropylcellulose (HPC). The disintegrant is preferably selected from carboxymethylcellulose calcium (Ca-CMC), sodium starch glycollate, polacrylin potassium or cross-linked polyvinylpyrrolidone And the sweetening agent is selected from white sugar, fructose, sorbitol or aspartame. Examples of the stabilizer include sodium carboxymethylcellulose sodium (Na-CMC), beta-cyclodextrin (beta -cyclodextrin) white bee's wax or xanthan gum and the preservative is selected from the group consisting of methyl p-hydroxy benzoate, methlparaben, propyl p-hydroxybenzoate, propylparaben, or potassium sorbate potassium sorbate).

In addition, the present invention provides a microbial preparation for controlling an animal pathogenic bacterium containing the Lactobacillus perfumant SJ8256 strain, a culture thereof or a mixture thereof as an active ingredient.

The present invention also provides a feed additive comprising the Lactobacillus perfumant SJ8256 strain, a culture thereof or a mixture thereof as an active ingredient.

It is preferable, but not limited, that the strain, the culture solution thereof, or a mixture thereof exhibit antagonism to either or both of Salmonella sp. And E. coli .

Preferably, the strain, the culture or the mixture thereof is contained in an amount of 10 to 20 parts by weight, but is not limited thereto. It is preferable that the culture solution contains the culture supernatant obtained by culturing the lactobacillus fermentum SJ8256 strain of the present invention and the culture supernatant from which the strain has been diluted, more preferably 0.5 to 500 times diluted. It is preferable that the composition of the culture liquid further includes not only the components required for a conventional Lactobacillus strain, but also all the components synergistically acting on the growth of Lactobacillus.

The livestock pathogenic bacterium is preferably, but not limited to, one selected from the group consisting of infectious diarrhea, gastroenteritis, inflammatory bowel disease, neurodegenerative inflammatory syndromes, small intestine microbial growth and intestinal diarrhea.

The strain, its culture or a mixture thereof may be selected from the group consisting of amikacin, amoxicillin / clavulanic acid, ampicillin, cephalothin, ciprofloxacin, gentamycin ), Neomycin, penicillin, sulfamethoxazole / trimethoprim, streptomycin, tetracycline, vancomycin, kanamycin, erythromycin erythromycin, clindamycin, chloramphenicol, synercid, quinupristin and dalfopristin combination, linezolid, trimethoprim, ciprofloxacin and spectinomycin. Which is capable of replacing all antibiotics used in livestock pathogenic bacteria in the art, Substitution of one or more selected from the group consisting of carcin, amoxicillin-clavulanic acid, ampicillin, cephalosin, ciprofloxacin, gentamicin, neomycin, penicillin, sulfamethoxazole / trimethoprim, streptomycin and tetracycline But is not limited thereto.

In another specific embodiment of the present invention, the present inventors have found that the Lactobacillus perfumant SJ8256 strain is in the form of Escherichia coli and Salmonella spp. (Table 1). It was confirmed that the antimicrobial effect was remarkably enhanced when compared with various antibiotics currently used.

In order to confirm the antimicrobial activity of the pathogenic microorganisms in vivo, the inventors of the present invention, in order to confirm the antimicrobial effect in vivo, were fed a feed additive to which lactobacillus fermentum SJ8256 strain was added to pigs that caused diarrheal diseases by aggressive inoculation of Escherichia coli and Salmonella, As a result, it was confirmed that Lactobacillus perfumant SJ8256 suppresses the growth of Escherichia coli and Salmonella spp. In vivo, and thus, it can be easily applied to multiple pathogenic microorganisms.

Therefore, the Lactobacillus perfumant SJ8256 strain of the present invention exhibits a strong antimicrobial effect against the above-mentioned multi-resistant pathogens and exhibits a remarkably elevated effect as compared with various antibiotics currently used. Therefore, the Lactobacillus perfumant SJ8256 strain , A culture solution thereof, or a mixture thereof can be usefully used as an effective ingredient of a microorganism preparation for controlling an animal pathogenic bacterium.

The feed composition may contain only cultures from which the cells have been removed, and may be prepared so that the cells are mixed or contain only cells.

The feed composition of the present invention has the effect of replacing existing antibiotics and inhibiting the growth of harmful food pathogenic bacteria to improve the health condition of the animal, improve the weight gain and meat quality of the livestock, increase the milk yield and immunity. The feed composition of the present invention can be produced in the form of a fermented feed, a compound feed, a pellet form, a silage reed, or the like. The effective amount of the lactobacillus fermentum SJ8256 strain of the present invention added to the feed can be about 10 ⁵ cfu / g to about 10 ¹¹ cfu / g, and specifically about 10 ⁶ cfu / g to about 10 ¹⁰ cfu / g And most specifically about 10 ⁷ cfu / g to about 10 ⁹ cfu / g, but is not limited thereto.

The fermented feed can be prepared by fermenting an organic material by adding various microbial groups or enzymes other than the lactobacillus fermentum SJ8256 strain of the present invention, and the compound feed can be prepared by mixing various kinds of general feeds and lactobacillus fermentum SJ8256 Can be produced by mixing strains. The pellet-shaped feed can be produced by applying heat and pressure to the compound feed or the like in a pelletizer, and the silage can be produced by fermenting a chewing feed with the microorganism according to the present invention. The wet fermented feed is prepared by collecting and transporting organic matter such as food waste, mixing the excipient and the excipient for moisture control at a certain ratio, fermenting at a suitable temperature for fermentation for more than 24 hours, And the like. The fermented dried feed can be prepared by adjusting the wet fermented feed so that the moisture content is 30% to 40%.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

< Example  1> Isolation and Identification of Antimicrobial Activity Strains

<1-1> Isolation of an antibacterial activity strain

In order to isolate a novel strain exhibiting an antimicrobial activity against a pathogenic microorganism in the intestine, the microorganism of the genus Lactobacillus was isolated.

Specifically, MRS broth;: by the addition of acetic acid (acetic acid) to (Man, Rogosa and Sharpes broth product name Lactobacilli MRS broth Difco ^TM, Becto, USA) was prepared in Lactobacillus separation medium. Then, the prepared medium was inoculated with various samples at 10% (v / v) and cultured at 37 DEG C for 48 hours. After the incubation, a culture medium was obtained and serial dilution was performed with 0.85% sterile saline solution to prepare a diluted culture medium diluted to 10 ^-6 to 10 ^-7 times. 0.1 ml of the diluted culture medium was added to 0.1% (w / v) calcium carbonate (CaCO ₃ ), cultured at 37 ° C for 3 days, and colony forming a transparent ring by acid production was selected, The cells were subcultured in a fresh MRS solid medium and purified. The pure isolates were cultured in MRS liquid medium and then stored at -70 ° C in 20% glycerol.

<1-2> Identification of antimicrobial active strains

In order to confirm whether the strain isolated with the antimicrobial activity is a novel strain, the isolated Lactobacillus sp. Strain was identified.

Specifically, the genomic DNA was extracted from the strain isolated in Example <1-1>, and the nucleotide sequence was analyzed to obtain the entire nucleotide sequence of 16S rRNA. The nucleotide sequence of the 16S rRNA was aligned using a blast N program of the National Center for Biotechnology Information (NCBI), and was identified as a strain having 99% or more homology. In addition, based on the 16s rRNA gene sequence, phylogenetic positions with strains reported as Lactobacillus sp. Were plotted using a phylogenetic tree using neighbor-joining, Lactobacillus strains with the closest relationship to one strain were identified.

As a result, as shown in FIG. 1, the antimicrobial active strain has a 16r rRNA nucleotide sequence of SEQ ID NO: 1 with a total size of 1,568 bp, which is 99% or more homologous to Lactobacillus fermentum , The antimicrobial activity strain of the present invention was confirmed to be a novel strain. Further, the novel strain was designated as Lactobacillus perfumant SJ8256, and the entire genomic sequence was registered in the NCBI gene bank (GeneBank, www.ncbi.nlm.nih.gov/genbam), and the accession no. It has been granted the registration number of KC759437 and deposited on November 7, 2013 with the Korea Biotechnology Research Institute (KCTC 12516BP). In addition, it was confirmed that the Lactobacillus fermentum SJ8265 strain was a strain located very close to the genus Lactobacillus strains genetically and systemically. Among them, strains belonging to Lactobacillus fermentum sp. (Fig. 1). &Lt; tb &gt;&lt; TABLE &gt;

< Example  2> Lactobacillus Fermantum SJ8256 Identification of antimicrobial activity

In order to confirm whether the novel Lactobacillus fermentum SJ8256 exhibits antibacterial activity against pathogenic microorganisms in the intestines, it is recommended that Lactobacillus fermentum SJ8256 ( E. coli ) and E. coli ( Salmonella ) Was confirmed by performing a well diffusion assay.

Specifically, a NB broth soft agar medium containing 0.8% agar was prepared, a well was prepared in the medium using a sterilized cork borer, % (v / v) Escherichia coli (Korean Gene Bank, sale from KCTC), Salmonella sp .; An indicator plate was prepared by streaking Salmonella typhimurium KCTC1925 (sold in KCTC) or S. typhimurium KCTC1926 (sold in KCTC), respectively. Then, 100 쨉 L of Lactobacillus perfumant SJ8256 cultured in Example 1 was inoculated and cultured at 37 째 C for 24 hours to confirm growth inhibition of Lactobacillus perfumant SJ8256 against E. coli and Salmonella. As a positive control, enterococcus paclum ( Enterococcus faecium), Lactobacillus putting momentum into (Lactobacillus fermentum sp.) and Pediococcus acidilactici sp. were used. As a negative control, distilled water was used to inhibit the growth inhibition against E. coli and Salmonella in the same manner as above.

As a result, as shown in Fig. 2, it was found that both the Lactobacillus fermentum SJ8256 and the positive control strain inhibited the growth of Escherichia coli and Salmonella between the wells, , Lactobacillus perfumant SJ8256 has antibacterial activity against the growth of pathogenic microorganisms irrespective of the kinds of pathogenic microorganisms (FIG. 2).

< Example  3> Lactobacillus Fermantum SJ8256  And commercial antibiotics ( antibiotics )of Antimicrobial activity  compare

In order to confirm the antimicrobial activity of Lactobacillus perfumant SJ8256, which shows antimicrobial activity against intestinal pathogenic microorganisms, antimicrobial activity of Lactobacillus perfumant SJ8256 was compared with conventional antibiotics by disc diffusion assay .

Specifically, an NB soft cloth containing 0.8% agar was prepared, and an instruction medium was prepared by dissolving 1% (v / v) Escherichia coli, Salmonella genus, S. typhimurium KCTC1925 or S. typhimurium KCTC1926 . Then, 100 μl of Lactobacillus fermentum SJ8256 cultured in the above <Example 1> was absorbed and dried on each paper disk, and the microorganism was cultured at 37 ° C And incubated in an incubator for 24 hours to measure the size of the growth inhibition rings around the disc.

Comparison of antimicrobial activities of Lactobacillus fermentum SJ8256 and commercial antibiotics Antibiotic Growth inhibition diameter (mm) Antibiotic treatment
density Escherichia coli Salmonella genus Amikacin 17 ± 1 13 ± 1 30.00 g Amoxicillin-clavulanic acid
(amoxicillin / clavulanic acid) 9 ± 1 7 ± 1 30.00 g Ampicillin - - 10.00 [mu] g Cephalothin - - 30.00 g Ciprofloxacin - 13 ± 1  5.00 [mu] g Gentamycin 12 ± 1 10 ± 1 10.00 [mu] g Neomycin 12 ± 1 10 ± 1 30.00 g Penicillin - - 10 IU / IE / UI Sulfamethoxazole / trimethoprim
(sulfamethoxazole / trimethoprim) - - 23.75 [mu] g Streptomycin 17 ± 1 -  1.25 [mu] g Tetracycline 7 ± 1 7 ± 1 10.00 [mu] g Lactobacillus fermentum SJ8256 18 ± 1 18 ± 1 100 μl

As a result, as shown in the above Table 1, amikacin, amoxicillin-clavulanic acid, gentamicin, neomycin and tetracycline exhibited growth of 7 to 17 mm in both the indicator medium of both pathogenic microorganisms of Escherichia coli and Salmonella Aminopterin, penicillin, and sulfamethoxazole / trimethoprim showed no antimicrobial activity against E. coli and Salmonella, while ciprofloxacin showed only a low inhibitory effect against Salmonella spp. , And it was confirmed that streptomycin forms a growth inhibitory ring only against E. coli. On the other hand, Lactobacillus perfumant SJ8256 formed 18 ㎜ inhibition of growth in both Escherichia coli and Salmonella spp., Indicating that the antibiotic activity against Escherichia coli and Salmonella spp. Exhibited by conventional commercial antibiotics was more remarkable than that of Escherichia coli and Salmonella spp. Table 1).

< Example  4> Lactobacillus Fermantum SJ8256 In vivo ( In vivo ) Confirmation of antibacterial effect

In order to confirm the antimicrobial effect of Lactobacillus fermentum SJ8256 on pathogenic microorganisms in vivo, the lactobacillus fermentum SJ8256 caused by beta-glucan in piglets causing diarrheal disease as an aggressive infection of Salmonella spp. The effect of the branch office was confirmed.

Specifically, Lactobacillus fermentum SJ8256 strain isolated in Example 1 was added to a general feed at a concentration of 10 ⁶ cfu / kg, and then fed to 10 experimental piglets. For use as a control group, 10 litter pigs were fed with the control diet without the strain. Two weeks after the start of feeding, Salmonella strains were aggressively infected with the above-mentioned experimental group and control group pigs to induce diarrheal disease. In the experimental group, feeds supplemented with strains having the same concentrations as those supplied before the aggressive infection were fed, The antimicrobial effect was confirmed by comparing the presence or absence of diarrheal disease.

As a result, as shown in the following Table 2, diarrheal disease started to appear from 2 days after aggressive infection of Salmonella strain in the control group, and 4 days later, all 10 mice in the control group developed diarrheal disease, And it was confirmed that it was alleviated over time. In contrast, in the experimental group fed with the Lactobacillus perfumant SJ8256 strain after the aggressive infection of the Salmonella strain, Lactobacillus perfumant SJ8256 effectively inhibited the growth of Salmonella spp. In vivo (Table 2). As shown in FIG.

In vivo antimicrobial effect of Lactobacillus fermentum SJ8256 Experimental group and
Control group ^* Diarrheal disease-inducing piglets ^** 1 day 2 days 3 days 4 days 5 days 6 days 7 days Normal feed 0/10 0/10 0/10 0/10 0/10 0/10 0/10 Experimental group 0/10 0/10 0/10 0/10 0/10 0/10 0/10 Control group 0/10 8/10 9/10 10/10 10/10 8/10 2/10

 * Non-test group: general feed group without Salmonella aggressive infection;

   Experimental group: Lactobacillus fermentum SJ8256 treated feedstuff group after aggressive infection of Salmonella; And

   Control group: General feed group after salmonella aggressive infection.

** Indicates the number of piglets in which the diarrheal disease was present in 10 of the experimental and control groups after aggressive inoculation of E. coli and Salmonella.

Korea Biotechnology Research Institute KCTC12516BP 20131107

<110> Korea Research Institute of Bioscience and Biotechnology <120> A novel Lactobacillus fermentum SJ8256 and use thereof <130> 13P-08-011 / P2013-166-KR <160> 1 <170> Kopatentin 2.0 <210> 1 <211> 1568 <212> DNA <213> Lactobacillus fermentum <400> 1 aaggaggtga tccatccaca ggttctccta cggctacctt gttacgactt caccctaatc 60 atctgtccca ccttaggcgg ctggctccta aaaggttacc ccaccgactt tgggtgttac 120 aaactctcat ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc accgcggcat 180 gctgatccgc gattactagc gattccgact tcgtgcaggc gagttgcagc ctgcagtccg 240 aactgagaac ggttttaaga gatttgcttg ccctcgcgag tttgcgactc gttgtaccgt 300 ccattgtagc acgtgtgtag cccgggtcat aaggggcatg atgatctgac gtcgtcccca 360 ccttcctccg gtttgtcacc ggcagtctca ctagagtgcc caacttaatg ctggcaacta 420 gtaacaaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga 480 cgaccatgca ccacctgtca ttgcgttccc gaaggaaacg ccctatctct agggttggcg 540 caagatgtca agacctggta aggttcttcg cgtagcttcg aattaaacca catgctccac 600 cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg 660 cggagtgctt aatgcgttag ctccggcact gaaagggcgg aaaccctcca acacctagca 720 ctcatcgttt acggcatgga ctaccagggt atctaatcct gttcgctacc catgctttcg 780 agtctcagcg tcagttgcag accaggtagc cgccttcgcc actggtgttc ttccatatat 840 ctacgcattc caccgctaca catggagttc cactaccctc ttctgcactc aagttatcca 900 gtttccgatg cacttctccg gttaagccga aggctttcac atcagactta gaaaaccgcc 960 tgcactctct ttacgcccaa taaatccgga taacgcttgc cacctacgta ttaccgcggc 1020 tgctggcacg tagttagccg tgactttctg gttaaatacc gtcaacgtat gaacagttaa 1080 tctcatacgt gttcttcttt aacaacagag ctttacgagc cgaaaccctt cttcactcac 1140 gcggtgttgc tccatcaggc ttgcgcccat tgtggaagat tccctactgc tgcctcccgt 1200 aggagtatgg gccgtgtctc agtcccattg tggccgatca gtctctcaac tcggctatgc 1260 atcatcgcct tggtaggccg ttaccccacc aacaagctaa tgcaccgcag gtccatccag 1320 aagtgatagc aagaagccat cttttaagcg ttgttcatgc gaacaacgct gttatgcggt 1380 attagcatct gtttccaaat gttgtccccc gcttctgggc aggttaccta cgtgttactc 1440 acccgtccgc cactcgttgg cgaccaaaat caatcaggtg caagcaccat caatcgattg 1500 ggccaacgcg ttcgacttgc atgtattagg cacaccgccg gcattcatcc tgagccatga 1560 tcaactct 1568

Claims (11)

Lactobacillus fermentum SJ8256 strain deposited with accession number KCTC 12516BP.
The Lactobacillus fermentum SJ8256 strain according to claim 1, wherein the strain has the 16S rRNA base sequence set forth in SEQ ID NO: 1.
The Lactobacillus fermentum SJ8256 strain according to claim 1, wherein the strain exhibits antagonism against E. coli or Salmonella sp. Strain.
A microorganism preparation containing the lactobacillus fermentum SJ8256 strain of claim 1, a culture thereof or a mixture thereof as an active ingredient.
The microbial preparation according to claim 3, wherein the strain, the culture solution thereof, or the mixture thereof is contained in an amount of 10 to 20 parts by weight.
Lactobacillus fermentum SJ8256 strain deposited with accession number KCTC 12516BP, a culture thereof, or a mixture thereof as an active ingredient, and a microorganism preparation for controlling an animal pathogenic bacterium.
[Claim 7] The microorganism preparation for controlling an animal pathogenic bacterium according to claim 6, wherein the strain, a culture solution thereof or a mixture thereof exhibits antagonism to E. coli or a Salmonella sp. Strain.
[7] The microbial preparation for controlling bacterial pathogenic bacteria according to claim 6, wherein the strain, the culture solution thereof or a mixture thereof is contained in an amount of 10 to 20 parts by weight.
[Claim 7] The microorganism preparation for controlling bacterial pathogenic bacteria according to claim 6, wherein the strain, the culture solution thereof, or the mixture thereof is diluted to 0.5 to 500 times.
The method according to claim 6, wherein the livestock pathogenic bacterium induces any one selected from the group consisting of infectious diarrhea, gastroenteritis, inflammatory bowel disease, neurodegenerative inflammatory syndromes, small intestine microbial growth and intestinal diarrhea, Microorganism preparation for the control of pathogenic bacteria.
A feed additive comprising the Lactobacillus fermentum SJ8256 strain of claim 1, a culture thereof or a mixture thereof as an active ingredient.

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