KR20150005423A - Nature-friendly composition for the pre-treatment of biomass, process for the pre-treatment of biomass by using the same, and functional products manufactured by using the pre-treated biomass - Google Patents

Abstract

The present invention relates to a biomass pretreatment method, and more particularly, to an eco-friendly composition for pretreatment of biomass hydrolysis, which can be commonly applied to tree-based biomass such as pine and oak as well as herbaceous biomass such as rice straw, , A biomass pretreatment method using the composition, and a method for producing a functional product using the pretreated biomass.

Description

TECHNICAL FIELD The present invention relates to an eco-friendly composition for pretreatment of biomass hydrolysis, a biomass pretreatment method using the composition, and a functional product prepared from the biomass pretreated by the above method biomass by using the same, and functional products manufactured by using the pre-treated biomass}

The present invention relates to a biomass pretreatment method, and more particularly, to an eco-friendly composition for pretreatment of biomass hydrolysis, which can be commonly applied to tree-based biomass such as pine and oak as well as herbaceous biomass such as rice straw, , A biomass pretreatment method using the composition, and a method for producing a functional product using the pretreated biomass.

Plant cell walls are composed of lignocellulosic material, the structure of which is briefly represented by cellulose (linear glucose polymer), hemicellulose (highly branched heteropolymer) and lignin (cross-linked aromatic polymer).

Binding of polysaccharides (cellulose and hemicellulose) and non-polysaccharide (lignin) components is a major cause of mechanical and biological resistance. Cellulose, which is the most abundant polysaccharide on the planet, is a polymer in which cellobiose (D-glucopyranosyl-beta-1,4-D-glucopyranose), which occupies more than 50% of the weight of wood, is arranged in a very orderly manner.

In the cellulose chain forming a long bundle of microfibrils of molecules, wood cellulose has about 10,000 glucose units. Cellulosic materials consist of amorphous regions in which chemical and biochemical reactions are easy, microfibril sequences are irregularly arranged, and microfibril order crystals arranged in a relatively difficult order.

The cellulolytic enzyme can hydrolyze the cellulose polymer into glucose, which is a monomer, and the glucose thus produced is naturally fermented with ethanol by saccharomyces cerevisiae, a six-sugar fermentation yeast. Thus, these biocatalysts (enzymes) are central to biomass ethanol technology.

Hemicellulose is a binding material of cellulose and lignin. Wood hemicellulose contains not only high amounts of xylose, but also glucose, mannose, galactose and arabinose It is a highly branched heteropolymer composed of uronic acid, short chain (polymerization degree 100 to 200). Hemicellulose is linked via 1,3, 1,6 and 1,4 glucosidic bonds and is often acetylated.

On the other hand, lignin is a three-dimensional polyphenolic structure of a phenylpropanoid unit attached with 0-2 methoxy groups derived from p-hydroxycinnamic alcohol. Lignin is hydrophobic and very resistant to chemical and biological degradation. Since lignin is structurally surrounded by hemicelluloses and cellulose fibrils, it provides resistance to biodegradation of these materials and acts as a bonding agent between plant cells as an amorphous material. The non-structural components (phenol, tannin, fats, sterols, sugars, starches, proteins and ashes) of plant tissues generally account for less than 5% of the dry weight of the wood.

Thus, herbaceous and tree-based biomass is a rich and renewable resource and has great potential as a substrate for fermentation, but it has been shown that biomass hydrolysis, such as depolymerization of biomass polysaccharides into fermentable sugars, , The above-mentioned structural characteristics necessitate a pretreatment step such as steam explosion, mild acid treatment, strong acid treatment, ammonia treatment, or hydrooxide treatment.

This pre-treatment step is a very important economic and technological unit process in the entire process of bioethanol production. The purpose of the pretreatment step is to reduce the size of the raw material and increase the reactivity at the time of saccharification so that hydrolysis of the polysaccharide after the pretreatment and ethanol fermentation To increase efficiency.

Therefore, no matter what treatment method is selected in the pre-treatment step, the pretreatment method should be carried out with an environmentally and economically feasible technique. Also, the determination of the pretreatment method should be influenced not only by process yield, production parameters, but also by environmental friendliness, process dependency and cost.

The present inventors have repeatedly studied on the production of bioethanol using biomass, and as a result, they have completed the present invention by developing an eco-friendly composition for pretreatment of new biomass hydrolysis.

Therefore, it is an object of the present invention to provide a method for removing lignin which is environmentally friendly and comparatively low in environmental toxicity as compared with inorganic acids (sulfuric acid, phosphoric acid, hydrochloric acid, etc.) used in conventional pretreatment methods, Which is capable of improving the saccharification efficiency by the cellulolytic enzyme, and to provide an eco-friendly composition for pretreatment of biomass hydrolysis.

Another object of the present invention is to provide a process for producing a biomass-derived functional product including bioethanol by reducing an amount of enzyme saccharification time and an enzyme used in a posterior process by using an environmentally friendly composition comprising a carboxylic acid and hydrogen peroxide, Which is a biomass hydrolysis pretreatment method.

It is still another object of the present invention to provide a method for producing high value-added biomass-derived functional products including biomass pretreated with biomass and biomass pretreated with the pretreatment method, and functional products prepared by the above- .

The objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to accomplish the object of the present invention, first, the present invention is characterized in that lignin contained in the biomass is removed in a state containing a carboxylic acid and hydrogen peroxide solution and maintaining physicochemical properties of a carboxylic acid and hydrogen peroxide An eco-friendly composition for pretreatment of biomass hydrolysis is provided.

In a preferred embodiment, the carboxylic acid is at least one selected from the group consisting of formic acid, acetic acid, propionic acid, and butyric acid.

In a preferred embodiment, the biomass comprises at least one of an herbaceous biomass and a tree-based biomass.

The present invention also relates to a method for preparing a green body composition, comprising: preparing an environmentally friendly composition by mixing a carboxylic acid and hydrogen peroxide; Adding the prepared green composition to the biomass and mixing the biomass; Heat-treating the mixture of the biomass and the environmentally friendly composition at 60 ° C to 100 ° C; And filtering the heat-treated biomass to obtain a solid material. The method for pretreating biomass hydrolysis according to the present invention comprises the steps of:

In a preferred embodiment, the carboxylic acid is at least one selected from the group consisting of formic acid, acetic acid, propionic acid, and butyric acid.

In a preferred embodiment, the mixture of the biomass and the environmentally friendly composition comprises the biomass and the environmentally friendly composition in a weight ratio of 1: 5 to 1:10.

In a preferred embodiment, the heat treatment is performed in a range of 2 hours to 6 hours.

In a preferred embodiment, the biomass is one of herbaceous biomass, tree-based biomass, and complex biomass mixed with herbaceous system and woody system, and is ground to a size of 1-10 mm.

In a preferred embodiment, the method further comprises washing the filtered solids.

The present invention also provides a pretreatment biomass which is pretreated by any one of the biomass hydrolysis pretreatment methods described above so that the lignin content is within a range of 0 wt% to 3 wt% of the total lignin content of the biomass do.

The present invention also provides a method for producing a biomass-derived functional product, which comprises a saccharification step of saccharifying the pretreatment biomass described above.

In addition, the present invention provides a biomass-derived functional product, which is produced by the above-mentioned method for producing a functional product.

In a preferred embodiment, the functional product includes at least one of glucose, xylose, mannose, galactose, rhamnose, arabinose, bioethanol, and acetic acid.

According to the present invention, by using an eco-friendly composition containing a carboxylic acid such as acetic acid and hydrogen peroxide less in environmental toxicity and comparatively low in toxicity than inorganic acids (sulfuric acid, phosphoric acid, hydrochloric acid, etc.) used in conventional pretreatment methods, Only lignin, which is an element, can be partially or completely removed, thereby improving the saccharification efficiency by the cellulolytic enzyme, ultimately decreasing the saccharification time of the enzyme and the amount of enzyme used, thereby reducing the production cost of bioethanol.

The technical effects of the present invention are not limited to those mentioned above, and other technical effects not mentioned can be clearly understood by those skilled in the art from the following description.

FIG. 1 is a photograph showing biomass change state before and after pretreatment of biomass with an environmentally friendly composition according to an embodiment of the present invention. FIG.
FIG. 2 is an optical microscope photograph showing lignin and cellulose birefringence in a biomass before and after pretreatment with biomass as an environmentally friendly composition according to another embodiment of the present invention;
FIG. 3 is a graph showing an enzyme saccharification rate according to mixing ratio of acetic acid and hydrogen peroxide at the pretreatment with an environmentally friendly composition according to another embodiment of the present invention,
FIG. 4 is a graph showing an enzyme saccharification rate according to a pretreatment temperature in biomass pretreatment with an eco-friendly composition according to another embodiment of the present invention,
FIG. 5 is a graph showing an enzyme saccharification rate according to a pretreatment time in biomass pretreatment with an eco-friendly composition according to another embodiment of the present invention;
FIG. 6 is a graph showing an enzyme saccharification rate of biomass pretreated with an environmentally friendly composition according to another embodiment of the present invention,
FIG. 7 is a graph showing an enzyme saccharification rate according to an enzyme concentration and an enzyme saccharification time in a biomass pretreated with an environmentally friendly composition according to another embodiment of the present invention,
FIG. 8 is a graph showing changes in the saccharification efficiency of the pretreatment biomass obtained after pretreatment of mixed biomass (rice straw, oak, pine) as an environmentally friendly composition according to another embodiment of the present invention,
9 is a graph showing the result of simultaneous saccharification and fermentation by enzyme saccharification and yeast in pretreatment biomass obtained after pretreatment of each biomass (rice straw, oak, pine) with an environmentally friendly composition according to another embodiment of the present invention .

Although the terms used in the present invention have been selected as general terms that are widely used at present, there are some terms selected arbitrarily by the applicant in a specific case. In this case, the meaning described or used in the detailed description part of the invention The meaning must be grasped.

Hereinafter, the technical structure of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Like reference numerals used to describe the present invention throughout the specification denote like elements.

A technical feature of the present invention is finding a new use of environmentally friendly compositions comprising acetic acid and hydrogen peroxide water. That is, if the biomass hydrolysis pretreatment is carried out using the environmentally friendly composition of the present invention, lignin, which is an inhibitory factor of the enzyme saccharification contained in the biomass, can be effectively removed and the saccharification efficiency by the cellulose degrading enzyme can be improved. The eco-friendly composition of the present invention is less environmentally toxic than inorganic acids such as sulfuric acid, phosphoric acid, and hydrochloric acid used in conventional biomass hydrolysis pretreatment methods, and is not only environmentally friendly but also relatively low in cost, When the saccharification process is carried out using the biomass pretreated with the environmentally friendly composition of the present invention, the saccharification time and enzyme usage can be reduced.

Thus, the eco-friendly composition of the present invention includes a carboxylic acid and a hydrogen peroxide solution, for example, a carboxylic acid and a hydrogen peroxide number in a molar ratio of 0.5 to 7: 1. At this time, the purity of the carboxylic acid may be 99% or more and the concentration of hydrogen peroxide may be 20% by weight or more. When the molar ratio of carboxylic acid to hydrogen peroxide is more than 7: 1, the cellulose and hemicellulose contained in the lignocellulosic biomass are rapidly lost. When the molar ratio of carboxylic acid and hydrogen peroxide is less than 0.5: 1, the degree of delignification is low, .

The carboxylic acid contained in the eco-friendly composition for pretreatment of biomass hydrolysis of the present invention may be at least one selected from the group consisting of formic acid, acetic acid, propionic acid, and butyric acid, although any known carboxylic acid having a carboxyl group may be used .

The eco-friendly composition for pretreatment of biomass hydrolysis of the present invention can be used not only for herbaceous biomass such as rice straw, herbaceous reeds and reeds, but also for pretreatment of complex biomass mixed with herbaceous and woody as well as woody biomass such as pine and oak Can be performed.

The affinity composition for pretreatment of the biomass hydrolysis of the present invention may selectively or completely remove only the lignin, which is an inhibitory factor of the enzyme saccharification, contained in the biomass.

Next, the biomass hydrolysis pretreatment method of the present invention uses the above-described environmentally friendly composition, comprising the steps of adding an eco-friendly composition containing carboxylic acid and hydrogen peroxide to the biomass and mixing the same; Heat-treating the mixture of the biomass and the environmentally friendly composition at 60 to 100 캜; And filtering the heat-treated biomass to obtain a solid. In some cases, washing the filtered solid may further comprise washing the filtered solid.

At this time, the biomass is any one of herbaceous biomass, tree-based biomass, and complex biomass mixed with herbaceous system and woody system, and the ground state of particles having a particle size of 1-10 mm is the contact surface area with the affinity composition And the environmental composition may contain, for example, a carboxylic acid and a hydrogen peroxide solution in a molar ratio of 0.5 to 7: 1 as described above.

In addition, the mixture of the biomass and the environmentally friendly composition obtained by mixing the biomass with the environmentally friendly composition may contain the biomass and the environmentally friendly composition at a weight ratio of 1: 5 to 1:10. When the mixing ratio of the biomass to the environmentally friendly composition is 1: 5 or less, the viscosity of the reactant is increased, the thermal conductivity for delignification in the biomass is lowered and the uniform reaction is difficult to expect. When the biomass is 1:10 or more, The concentration is increased, resulting in the loss of cellulose and hemicellulose, and the amount of the eco-friendly composition to be added increases, which is economically disadvantageous.

The heat treatment may be carried out in the range of 50 to 100 占 폚 for 2 to 6 hours. Similar to the mixing ratio of the biomass and the environmentally friendly composition, when the temperature of the reactor is 50 ° C or less, the delignification is slow, and when the temperature is 100 ° C or more, cellulose and hemicellulose will be lost.

The step of filtering the solids after the heat treatment or washing the solids is not particularly described because all known filtration methods or washing methods can be used.

In addition, the present invention can provide a pretreatment biomass which is pretreated by any one of the biomass hydrolysis pretreatment methods described above. Since the pretreatment biomass has a lignin content of 0 wt% to 3 wt% of the total weight of the lignin contained in the biomass, an effective saccharification process can be performed immediately without pretreatment of biomass, .

Therefore, the functional product derived from the biomass of the present invention can be extremely easily prepared by performing the saccharification process using the pretreatment biomass described above. That is, since the saccharide obtained in the saccharification process as well as the hexose and the pentose such as various glucose and xylose obtained only by the saccharification process can be subjected to a fermentation process to easily obtain additional functional products such as bioethanol .

Example 1

80 ml of acetic acid and 20 ml of aqueous hydrogen peroxide were mixed to obtain Environmentally Friendly Composition 1 (acetic acid: hydrogen peroxide molar ratio 7: 1).

Example 2

Environmentally friendly composition 2 (acetic acid: hydrogen peroxide molar ratio: 2.6: 1) was obtained in the same manner as in Example 1 except that 60 ml of acetic acid and 40 ml of hydrogen peroxide were used.

Example 3

Environmentally friendly composition 3 (acetic acid: hydrogen peroxide molar ratio: 1.2: 1) was obtained in the same manner as in Example 1 except that 40 ml of acetic acid and 60 ml of hydrogen peroxide were used.

Example 4

Environmentally friendly composition 4 (acetic acid: hydrogen peroxide molar ratio: 0.4: 1) was obtained in the same manner as in Example 1 except that 20 ml of acetic acid and 80 ml of hydrogen peroxide were used.

Example 5

Environmentally friendly composition 5 (acetic acid: hydrogen peroxide molar ratio 1.7: 1) was obtained in the same manner as in Example 1 except that 50 ml of acetic acid and 50 ml of aqueous hydrogen peroxide were used.

Examples 6 to 9

Formic, Acetic, Propionic and Butylic were obtained in the same manner as in Example 5 except that formic acid, acetic acid, propionic acid and butyric acid were used respectively, .

Examples 10 to 13

50 ml of Eco-Friendly Compositions 1 to 4 were added to 5 g of biomass obtained by pulverizing pine trees with a particle size of 1-10 mm, followed by heat treatment at 80 ° C for 3 hours. The heat treated product was then filtered to obtain a solid form of pretreatment biomass 1 (A80H20), pretreatment biomass 2 (A60H40), pretreatment biomass 3 (A40H60), and pretreatment biomass 4 (A20H80).

Examples 14 to 17

50 ml of Eco-Friendly Composition 5 was added to 5 g of the biomass obtained by pulverizing pine trees with a particle size of 1-10 mm, and then 4 mixtures were prepared. The mixture was heat-treated at 50 ° C, 60 ° C, 70 ° C and 80 ° C for 3 hours Respectively. The heat-treated product was then filtered to obtain a pre-treatment biomass 5-1 (50 ° C), a pretreatment biomass 5-2 (60 ° C), a pretreatment biomass 5-3 (70 ° C), and a pretreatment biomass 5-4 (80 占 폚, Pine wood).

Examples 18 to 23

Five milligrams of biomass obtained by pulverizing pine trees with a particle size of 1-10 mm was added with 50 ml of Eco-Friendly Composition 5, and then 6 mixtures were prepared. The mixture was then stirred at 80 ° C for 1 hour, 2 hours, 3 hours, 4 hours, 5 hours , And heat treatment was performed for 6 hours. The heat-treated product was then filtered to obtain a pre-treatment biomass 5-5 (1), a pretreatment biomass 5-6 (2), a pretreatment biomass 5-7 (3), a pretreatment biomass 5-8 Pretreatment biomass 5-9 (5) and pretreatment biomass 5-10 (6) were obtained.

Examples 24 to 27

To 5 g of the biomass obtained by pulverizing pine trees with a particle size of 1-10 mm, 6 to 90 ml of an environmentally friendly composition were added, and the resulting mixture was heat-treated at 80 ° C for 3 hours. The heat treated product was then filtered to obtain a solid form of pretreatment Biomass 6, pretreatment Biomass 7, pretreatment Biomass 8, and pretreatment Biomass 9 (Butylic).

Example 28

A rice straw was obtained in the same manner as in Example 13 except that the biomass obtained by pulverizing rice straw with a particle size of 1-10 mm was used.

Example 29

(Oak wood) was obtained in the same manner as in Example 13 except that the biomass obtained by pulverizing the oak tree with a particle size of 1-10 mm was used.

Example 30

The same procedure as in Example 13 was followed to obtain a pretreated composite biomass, except that the composite biomass obtained by mixing the same volume of pulverized material of rice straw, oak, and pine with a particle size of 1-10 mm was used.

Experimental Example 1

The pretreatment biomass of Example 28 obtained by treating the pulverized material of Rice straw, Oak and Pine and pulverized material pulverized with a particle size of 1-10 mm by the pretreatment method of the present invention, R, the pretreatment biomass 5-O obtained in Example 29, and the pretreatment biomass 5-4 obtained in Example 17 were observed before and after the pretreatment, and the results are shown in FIG.

From FIG. 1, it can be seen that the pre-pretreatment biomass of the present invention, which is a control, has a specific color of wood. However, the pretreatment biomass obtained after the pretreatment of the present invention shows discoloration to white . This change is predicted to be due to the fact that the lignin contained in the biomass was completely or almost completely removed through the pretreatment method of the present invention.

Experimental Example 2

The components of the pretreated biomass 5-4 obtained by treating 5 g of the pine crush before the pretreatment by the pretreatment method of the present invention and the pine crushed material by the method described in Example 13 were analyzed and the results are shown in Table 1. [

(weight %) Organic solvent Holocellulose Lignin Ash Pine milled (control) 3.9 69.3 22.5 0.11 Pretreatment pine crush (pretreatment biomass 5-4) 0.0 82.2 0.5 0.11

From Table 1, it can be seen that the pretreatment biomass obtained by performing the pretreatment method of the present invention has higher solubility in cellulose and almost all of the lignin and organic solvent extract is removed so that the saccharification process is performed even if the pretreatment process does not require a separate impurity removal process It can be seen that it is very suitable for

Experimental Example 3

A part of the pretreatment biomass 5-4 obtained by treating the pine wood pulverization product before the pretreatment by the pretreatment method of the present invention and the pine wood pulverization product by the method described in Example 17 was used as a reagent capable of selectively dyeing lignin The distribution of lignin under an optical microscope and the structure of the cellulose under a polarization microscope were observed and the photograph is shown in Fig.

It can be seen from FIG. 2 that when the pretreatment method of the present invention is used, lignin is completely removed or almost completely removed.

Experimental Example 4

The pretreatment biomass 1 to pretreatment biomass 4 obtained in Examples 10 to 13 were respectively treated with enzymes (Cellulase: 10 FPU / g biomass, Xylanse: 2000 IU / g biomass) The saccharification was carried out and the resulting enzyme saccharification rate is shown in Fig.

It can be seen from FIG. 3 that if the eco-friendly composition for pretreatment of biomass hydrolysis of the present invention contains carboxylic acid and hydrogen peroxide water, the biomass pretreated with the environmentally friendly composition effectively removes lignin and organic solvent extract inhibited by glycation It can be seen that it has an effective saccharification rate. In particular, when the eco-friendly composition for pretreatment of biomass hydrolysis according to the present invention contains acetic acid and hydrogen peroxide at a molar ratio of 0.5 to 7: 1, the enzymatic saccharification rate is very high. Therefore, when the reaction time is the same, And the mixed molar ratio of the carboxylic acid and the hydrogen peroxide solution contained in the eco-friendly composition in which the organic solvent extract is removed can be confirmed.

Experimental Example 5

(Cellulase: 10 FPU / g biomass, Xylanse: 2000 IU / g biomass) was used as the pretreatment biomass 5-1 to the pretreatment biomass 5-4 obtained in Examples 14 to 17, The enzyme saccharification was performed for 24 hours, and the resulting enzyme saccharification rate was shown in FIG.

From FIG. 4, it can be seen that the heat treatment temperature during the pretreatment may be 50 ° C or higher, but higher than 60 ° C can obtain a better enzyme saccharification rate. Although not shown, when the heat treatment temperature is 100 ° C or higher in the pretreatment, the enzyme does not significantly affect the saccharification rate. Therefore, the heat treatment temperature can be lowered to 100 ° C or less in consideration of energy saving and sugar loss.

Experimental Example 6

(Cellulase: 10 FPU / g biomass, Xylanse: 2000 IU / g biomass) was used at a substrate concentration of 1%, respectively, from the pretreatment biomass 5-5 to the pretreatment biomass 5-10 obtained in Examples 18 to 23. The enzymatic saccharification was performed for 24 hours, and the resultant enzyme saccharification rate was shown in FIG.

From FIG. 5, it can be seen that the heat treatment time during the pretreatment may be one hour or more, but a better enzyme saccharification rate can be obtained when treated for 2 hours or more. Although not shown, the heat treatment time can be set to 6 hours or less in consideration of energy saving since the heat treatment time in pretreatment does not significantly affect the enzyme saccharification rate even when the heat treatment time is 6 hours or more.

Experimental Example 7

Pretreatment Biomass 6 (Formic), Pretreatment Biomass 7 (Acetic), Pretreatment Biomass 8 (Propionic), and Pretreatment Biomass 9 (Butylic) obtained in Examples 24 to 27 were used as enzyme (Cellulase: 10 FPU / g biomass, Xylanse: 2000 IU / g biomass) for 24 hours, and the resulting enzyme digestion rate is shown in FIG.

6 that even when formic acid, propionic acid, and butyric acid are contained in the eco-friendly composition for pretreatment of the present invention, they exhibit a better saccharification ratio than acetic acid, and thus, as in the eco-friendly composition of the present invention, It is very suitable to be used in the biomass hydrolysis pretreatment method.

Experimental Example 8

The pretreatment biomass 5-R obtained in Example 28, the pretreated biomass 5-O obtained in Example 29 and the pretreatment biomass 5-4 obtained in Example 17 were used as substrates The enzyme saccharification was carried out at different concentrations of 1% enzyme and saccharification time, and the resulting saccharification rate of the enzyme was shown in FIG.

That is, enzyme saccharification was performed for 12 hours using an enzyme (Cellulase: 5 FPU / g biomass, Xylanse: 1000 IU / g biomass), and the resulting rate of enzyme digestion was expressed as C / X 2.5 12 h. Cellulase: 5 FPU / g biomass, Xylanse: 1000 IU / g biomass) was used for enzyme saccharification for 24 hours. The resultant enzyme glycosylation rate was expressed as C / X 2.5 24 h, / g biomass, Xylanse: 2000 IU / g biomass) for 12 hours, and the resulting rate of enzyme digestion was expressed as C / X 5 12 h. Cellulase (10 FPU / g biomass, Xylanse: 2000 IU / g biomass) for 24 hours and the resulting rate of glycosylation of the enzyme was expressed as C / X 5 24h.

It can be seen from Fig. 7 that both the content of enzyme and the saccharification time affect the enzyme saccharification rate in case of rice straw, but the enzyme saccharification rate of pine, when the enzyme content is the same, . Also, when the enzyme content and saccharification time are increased, it can be seen that the enzymatic saccharification rate of the oak tree and pine tree is better than that of the rice straw, which is herbaceous system, among the pretreatment biomass pretreated with the pretreatment method of the present invention.

Experimental Example 9

The hybridization pretreatment biomass obtained in Example 30 was saccharified using an enzyme (Cellulase: 10 FPU / g biomass, Xylanse: 2000 IU / g biomass) at a substrate concentration of 1% Is shown in Fig.

From Fig. 8, it can be seen that the saccharification time of the enzyme is sufficient within 24 hours. In particular, since the enzyme saccharification is actually terminated at 12 hours, the saccharification time of the enzyme may be less than 12 hours.

Experimental Example 10

(10 FPU / g biomass, Xylanse: 2000 IU / g biomass) was performed for 24 hours at a concentration of 1% of the substrate in the complex pretreatment biomass obtained in Example 30 while the enzyme and yeast were used And the simultaneous saccharification fermentation was carried out for 24 hours, and the results are shown in FIG.

From Fig. 9, it can be seen that various saccharide compounds can be obtained when only enzyme saccharification is performed. When the simultaneous saccharification fermentation is carried out using enzymes and yeast, the sugar compound obtained in most saccharification steps is fermented, It can be seen that the conversion is done.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications will be possible.

Claims (13)

Wherein the lignin contained in the biomass is removed while maintaining the physico-chemical properties of the carboxylic acid and hydrogen peroxide, including carboxylic acid and hydrogen peroxide.
The method according to claim 1,
Wherein the carboxylic acid is at least one selected from the group consisting of formic acid, acetic acid, propionic acid, and butyric acid.
The method according to claim 1,
Wherein the biomass comprises at least one of an herbaceous biomass and a neck base biomass.
Preparing a green composition by mixing a carboxylic acid and hydrogen peroxide water;
Adding the prepared green composition to the biomass and mixing the biomass;
Heat-treating the mixture of the biomass and the environmentally friendly composition at 60 ° C to 100 ° C; And
And filtering the heat-treated biomass to obtain a solid material.
5. The method of claim 4,
Wherein the carboxylic acid is at least one selected from the group consisting of formic acid, acetic acid, propionic acid, and butyric acid.
5. The method of claim 4,
Wherein the mixture of the biomass and the environmentally friendly composition comprises the biomass and the environmentally friendly composition in a weight ratio of 1: 5 to 1:10.
5. The method of claim 4,
Wherein the heat treatment is performed in a range of 2 hours to 6 hours.
5. The method of claim 4,
Wherein the biomass is one of herbaceous biomass, tree-based biomass, and complex biomass mixed with herbaceous tree and woody tree, and is pulverized to a size of 1-10 mm.
5. The method of claim 4,
Further comprising the step of washing said filtered solid matter.
A pretreatment biomass according to any one of claims 4 to 9, which is pretreated by the biomass hydrolysis pretreatment method to have a lignin content of 0 wt% to 3 wt% of the total weight of the lignin contained in the biomass.
A method for producing a biomass-derived functional product, which comprises saccharifying the pre-treatment biomass of claim 10.
A biomass-derived functional product produced by the production method of claim 11.
13. The method of claim 12,
Wherein the functional product comprises at least one of glucose, xylose, mannose, galactose, rhamnose, arabinose, bioethanol, and acetic acid.

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