KR20140137757A - Method for preparing vitamin E from brown rice extracts of Nunkeunhukchal(black sticky rice with giant embryo) and a composition comprising the extracts - Google Patents

Abstract

The present invention relates to a method for producing vitamin E from an extract of a macroglobulinous giant embryo (eye shadow phloem), and a food composition enriched with vitamin E, which comprises an extract of a macroglobulin giant embryo or an extract thereof as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for preparing vitamin E from an extract of brown rice, and a composition comprising the extract, and a composition comprising the extract of Nunkeunhukchal (black sticky rice giant embryo) }

The present invention relates to a vitamin E-enriched food composition comprising as an active ingredient, a method for producing vitamin E from an extract of a giant-ray-giant embryo (glaucoma, Milyang 263), and an extract of a giant-eye giant embryo or an extract thereof.

Vitamin E is known as a tocopherol of the four types of α, β, γ, and δ, and tocotrienol, which are collectively referred to as tocotrienol, depending on the position of the methyl group. The important function of vitamin E in the human body is antioxidant, which functions to inhibit the oxidation of unsaturated fatty acids, which constitute a substance which is easily oxidized in the cell, especially the cell membrane, thereby preventing damage to the cell membrane and further damage to the tissue. In general, tocopherol in vegetable oil contains about 60% of α-tocopherol. In addition, α-tocopherol has the highest antioxidative potential among the four tocopherols, so that the biological activity of α-tocopherol has been reported to be closely related to their antioxidant capacity. Especially, α-tocopherol plays an important role not only as an antioxidant as a food additive but also because it has physiological functions and nutritional activities such as anti-infertility and anti-aging in the body, but it can not be synthesized in the body. In addition to the antioxidant activity of α-tocopherol, which blocks the free radical chain reaction, it inhibits the activity of protein kinase C (PKC) in biochemical aspects. It was observed in smooth muscle, platelet, mononuclear cells, (ICAM-1) and VCAM-1 (vascular cell adhesion molecule 1) by inhibiting the adhesion of monocytes to vascular endothelial cells by inhibiting the expression of cell adhesion molecules, specifically ICAM-1 (Intercellular Adhesion Molecule 1) . Among the eight kinds of vitamin E,? -Tocopherol has the highest activity and is a component of a commercially available vitamin E supplement, but? -Tocopherol is the most commonly consumed form in natural foods. In contrast to α-tocopherol, γ-tocopherol has a weak antioxidant ability due to the absence of an electron donating ability at the C5 position of the chromanol nucleus in the molecular structure, and the hemolytic hemolytic effect is about 10% of α-tocopherol. However, γ-tocopherol reduces nitric oxide, which is highly toxic in the body, to nitrogen oxide or binds as it is to form 5-nitro-γ-tocopherol to detoxify it, thereby inhibiting nitrogen dioxide-induced inflammation, protein, lipid and DNA damage . In addition, γ-tocopherol and its metabolite γ-carboxyethyl-hydroxychroman (γ-CEHC) have been reported to have anti-inflammatory effects.

In studies of vitamin E, research on tocotrienols is far less than tocopherol. Since the mid-1990s, it has been known to be 40 to 60 times stronger than tocopherol in terms of various physiological activities such as antioxidation, anti-cancer, improvement of hyperlipidemia, hypoglycemia, and atherosclerosis, and is being developed as a material for medicines and health supplements. Tocotrienol is contained in rice bran, palm oil, barley embryo, and corn. It acts to prevent or kill the spread of cancer cells, and by the production of free radicals and peroxidized lipids caused by oxidation, Is known to inhibit aging by inhibiting physiological activity loss, weakening of cell function and necrosis.

Under these circumstances, the present inventors have made efforts to find a method for producing vitamin E, particularly alpha -tocopherol, in high yield, and as a result, the content of vitamin E is high in the goby giant macrobenthos, which can be a source of vitamin E And completed the present invention.

It is an object of the present invention to provide a method for producing vitamin E, which comprises extracting an optic giant embryo with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, hexane and ethyl acetate.

It is another object of the present invention to provide a vitamin E-enriched food composition comprising an extract of the goby giant Macrobrachium as an active ingredient.

It is still another object of the present invention to provide a vitamin E-enriched food composition comprising an optically-anomic giant embryo as an active ingredient.

In order to achieve the above object, the present invention provides a method for producing vitamin E, which comprises extracting a macroglobulin giant embryo with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, hexane and ethyl acetate, ≪ / RTI >

In the present invention, the term "giant opium giant embryo" refers to black rice having a black waxy giant embryo. The giant embryo of the present invention is characterized by being a giant embryo that is both black and black. For example, a giant embryo of the genus Bacteriophage (YR23517Acp79) 263). Herein, it means that the amylose is composed of 2 to 10% of amylose and 90 to 98% of amylopectin. The giant embryo is a variant of mutant breeding which is 3 to 5 times larger than that of the conventional rice varieties, Rice, which is known to be concentrated more than 80%, is 3 to 5 times larger than common brown rice, meaning that rice has a very rich variety of nutrients.

 In the present invention, the above-mentioned goby giant embryonic rice is a rice containing a large amount of vitamin E, preferably Milyang 263, but is not limited thereto. For the method of breeding of the above-mentioned macroglobulinous giant embryo (Milyang 263) Korean Patent Registration No. 10-1201436. In addition, the giant-ray-giant embryo may be a giant-goblet giant embryo or a goblet giant embryo, preferably a goblet giant embryo, but is not limited thereto.

The term "brown rice " in the present invention means rice in which harvested rice is dried and thawed, and then rice husked with a rubber roller. For the purpose of the present invention, the brown rice refers to rice which has been dried and thawed and then the rice husk has been stripped, but the present invention is not limited thereto.

In addition, the rice bran of the giant-ray giant-ray embryo contains a large amount of vitamin E, and thus can be a useful supply source in the production of vitamin E.

The term "rice bran" in the present invention means a pulverization mixture such as peel (peel), seed coat, and bran layer (powder layer) Refers to a pulverulent mixture formed during the fermentation of an opaque macrobiotic brown rice containing vitamin E, but is not limited thereto. Since the rice bran contains a high amount of vitamin E, vitamin E can be extracted therefrom.

In the present invention, it was confirmed for the first time that vitamin E, particularly? -Tocopherol, is contained in a large amount in the extract of the macrophage giant fibroblast, as compared with that of ordinary rice or colored rice. Therefore, vitamin E can be produced by preparing an extract from the goby goblins and separating and purifying the vitamin E.

In the present invention, the term "vitamin E" is a fat-soluble vitamin, which serves to maintain the cell membrane and neutralizes active oxygen as an antioxidant. Specifically, it acts as an antioxidant to prevent the progress of the peroxidation of unsaturated fatty acids in the presence of unsaturated fatty acids in the cell membrane. α, β, γ, δ There are four forms of tocopherol and four types of tocotrienols, which are also present in eight forms.

The term "tocopherol" in the present invention is a form of vitamin E, and there are four types of?,?,?, And?. It is known that α-tocopherol has the highest antioxidative power, and α-tocopherol is known to have both physiological functions and nutritional activities such as anti-infertility and anti-aging in the body as well as the role of antioxidants as food additives ≪ / RTI >

In the present invention, the term "an extract of a macrophage macrophage" means an extract obtained by extracting the macrophage macrophage. The above-described extract of the giant-bangchuan giant embryonic rice is prepared by grinding a giant-ray-like macrophyllotaxis rice giant rice bran, rice bran, rice or roasted brown rice and then subjecting the ground product to water, hexane, ethyl acetate, an alcohol having 1 to 4 carbon atoms, , But the present invention is not limited thereto. The alcohol having 1 to 4 carbon atoms is not limited to methanol, ethanol or butanol.

The extract may be an extract which is extracted using an extraction method such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction. The filtrate is subjected to filtration under reduced pressure, the filtrate is concentrated under reduced pressure, and water, alcohol, hexane, ethyl Acetate, or a mixed solvent thereof. However, the present invention is not limited to this, as long as it is an extract of a macrophyllotaxic giant embryo including vitamin E. However, the extract, the diluted solution or the concentrate of the extract or the extract may be dried , Or a preparation or a purified product thereof.

In addition, the extract of the above-mentioned giant-ray bombardment giant embryo may be one derived from a processed product of the form of goby giant embryonic rice rice, brown rice, fried brown rice or rice bran.

As described in Production Example 1, rice bran can be prepared by rice bran gypsum and starchy brown rice starch, but the present invention is not limited thereto. Also, as shown in Example 3, the roasted brown rice can be puffed while being rotated at a temperature of 155 to 160 ° C for 5 to 20 minutes, but the present invention is not limited thereto.

In addition, the manufacturing method of the present invention may further include a step of separating and purifying vitamin E from the extract of the giant-bamboo shoot.

In this case, the method for separating and purifying vitamin E can be carried out through various known methods for separating and purifying compounds. For example, the extract of vitamin A is obtained by concentrating and extracting the extract of brucite giant embryonic stem, , Followed by pure separation and purification using high performance liquid chromatography (HPLC).

In one embodiment of the present invention, it was confirmed that the content of vitamin E, in particular,? -Tocopherol was superior to that of ordinary rice or colored rice (Examples 1 and 2), and the processing of rice goby The fried brown rice extract also had an excellent vitamin E content, and the content of fried brown rice was higher than that of rice (Example 3). Further, it was confirmed that the extract of the present invention has excellent antioxidative activity, and thus confirmed that vitamin E having antioxidant ability is contained in a high content (Example 6). In addition, it was confirmed that the giant rice bran of rice gobs was high in content of vitamin E, and could be a useful source of vitamin E (Example 7).

In another aspect, the present invention provides a vitamin E-enriched food composition comprising an extract of a macrophage macrobiite as an active ingredient.

The above-mentioned giant-ray giant-ray embryo, its extract and vitamin E are as described above.

Specifically, when the vitamin E-enriched extract of the present invention is used as a food composition, the extract or its fractions can be directly added or used together with other foods or ingredients, Can be used. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

The kind of the food of the present invention is not particularly limited. Examples of the foods to which the extract of the present invention can be added include dairy products including rice grains, retort brown rice, meat, sausage, bread, chocolates, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, , Various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and may include foods in the conventional sense and used as feed for animals.

In addition to the above, the food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, , A carbonating agent used in a carbonated drink, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. The food may also be prepared in the form of tablets, granules, powders, capsules, solutions in liquid form, and the like according to known production methods. There are no particular restrictions on other components other than those containing the extract of the present invention as an active ingredient, and may include various conventional flavors or natural carbohydrates as additional ingredients.

In the present invention, it has been confirmed that the content of vitamin E in the extract of the giant-ray-giant Macrobrotta sp. Is excellent. Therefore, the food composition containing the vitamin E has a higher content of vitamin E than general or colored pigments.

In another aspect, the present invention provides a vitamin E-enriched food composition comprising an optically-anomic giant embryonic stem as an active ingredient.

The above-mentioned giant-ray biomass, vitamin E and food composition are as described above.

Specifically, the vitamin E-enriched macrophage giant embryo of the present invention can be added to the food composition. When the glioma of the present invention is used as a food additive, the glioma of the present invention can be used as it is, or it can be used together with other food or ingredients, and can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

The kind of the food of the present invention is not particularly limited. Examples of the foods to which the above-mentioned goby giant embryonic stem can be added include dairy products including cereal rice, retort brown rice, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, Various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and may include all foodstuffs in a conventional sense, and foods used as feedstuffs for animals.

Since the present invention contains a high amount of vitamin E, particularly tocopherol, it can be usefully used in the field to which tocopherol can be applied, for example, in the production of antioxidative foods or cosmetic compositions.

Fig. 1 is a graph showing the results of HPLC analysis of vitamin E content of the goby giant embryos.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are illustrative of the present invention, and the scope of the present invention is not limited by the following examples.

Manufacturing example  One: Giant biomass  Manufacture of brown rice

Macrogonosporous giant embryonic rice was prepared by cooking rice as a method of enhancing starch of brown rice and brown rice. In general, rice cookers and pressure cookers can be used as a general method of cooking rice. In this test, rice cooked using an electric rice cooker, which is a method used for a standard seasoning test, was used.

Specifically, after 200 g of rice of each variety was washed well, the amount of water was adjusted according to the water content of the rice (based on the rice water content of 14%, the amount of water was 1.2 times). After 20 minutes in water at 20 ° C, water was drained for 10 minutes, and rice was cooked using a conventional electric rice cooker. 10 to 15 minutes after the switch was turned off, the sample was immersed in a drying container for drying, and then dried using hot air drying at 40 ° C and freeze drying.

Manufacturing example  2: Giant biomass  Preparation of extract

To determine the contents of fat soluble vitamin E and its constituent components, alpha-tocopherol, alpha-tocotrienol, gamma -tocopherol and gamma-tocotrienol, which are contained in extracts of macrophyllotaxis and rice goby rice and rice, Extracted and concentrated in vacuo and used as analytical sample.

Manufacturing example  3: Giant Fried brown rice  Produce

In the present invention, a method of roasting a brown rice was used to produce a processed product having excellent palatability of the giant-ray-giant macroblock. The temperature was 150 to 170 ° C, and the roasting time was 5 to 20 minutes, And the results are shown in Table 1. < tb > < TABLE >

kind Expansion temperature (캜) Optimum temperature (℃) Remarks Japan 165-170 167 Burning occurs at temperatures above 170 ℃ Snowball
(Macrophyte giant embryo) 155-160 157 Burning occurs at temperatures above 160 ℃

As a result, as shown in Table 1, the production conditions of the fried brown rice excellent in palatability were found to be uniform puffing while rotating the brown rice at a temperature of 155 to 160 ° C and a roasting time of 5 to 20 minutes.

Example  One: Giant  Isolation and analysis of vitamin E

In order to isolate and analyze the vitamin E of the macroglobulin giant embryos, the samples obtained in Preparation Examples 1 to 3 were extracted.

Specifically, the powder was directly extracted under the extraction conditions shown in Table 2 below. The extraction solvent was extracted with one of hexane, ethyl acetate, and ethanol. The dried sample was dissolved in methanol and extracted again under the extraction conditions shown in Table 2 And the results of analysis of vitamin E of the giant-cell anthrax were shown in FIG.

As a result, as shown in FIG. 1, it was shown that the giant-ray-giant embryos of the present invention contained a high content of vitamin E.

Item Contents Extraction condition - 100 mg / 5 ml MeOH, 1 hr
- Extract with 5ml THF / 4 times and dry HPLC analysis conditions - Reverse phase gradient HPLC system
- HPLC: Waters 2695 alliance system
Column: C18 guard column (3 탆, 33 x 4.6 mm)
YMC C30 carotenoid column (3 [mu] m, 150 x 3.0 mm)
- Detector: PDA, 340, 292, 330nm
- Solvent A: methanol / tert-butyl methyl ether / water (85: 12: 3, v / v / v, with 1.5%
- Solvent B: methanol / tert-butyl methyl ether / water (8: 90: 2, v / v /
- Gradient procedure: flow rate 0.4 mL / min, 21 min linear gradient to 45% left A, 1 min hold, 11 min linear gradient to 5% left A, 4 min hold, 2 min linear gradient to 100% left

Example  2: Giant biomass  Check vitamin E content in brown rice

On the basis of the results of Example 1, vitamin E analysis was carried out by using a black-and-white giant embryo as a comparison group, a general beauty group as a comparative group, and a health red apple as a color pigment. The results are shown in Table 3 below.

Vitamin E
Japan
( mg / 100g) Health Promotion
( mg / 100g) Snowball
( Giant biomass )
( mg / 100g) α- Tocopherol 0.88 ± 0.09 1.0 ± 0.01 1.50 0.06 γ- Tocopherol 0.33 + 0.02 0.14 + 0.02 0.46 + 0.02 α- Tocotrienol 0.05 ± 0.01 0.07 ± 0.00 0.06 ± 0.00 γ- Tocotrienol 0.14 ± 0.01 0.14 ± 0.00 0.13 ± 0.00 Sum 1.40 + 0.04 1.34 ± 0.01 2.11 + 0.02

As a result, as shown in Table 3, α-tocopherol having the highest activity among tocopherols contained 0.88 mg / 100 g in the case of the normal rice and 1.0 mg / 100 g in the case of the healthy rice, And 1.5 mg / 100 g of alpha-tocopherol. In addition, the gamma-tocopherol contained 0.46 mg / 100 g of the goblet giant myoblasts, compared with 0.33 mg / 100 g of the general meals and 0.14 mg / 100 g of the health red ginseng. On the other hand, the contents of tocotrienol were similar between the α and γ.

The content of total vitamin E was found to be more than 50% higher than that of normal red rice, 1.34mg / 100g of healthy red rice, and 1.4mg / 100g of general rice, which is 2.11mg / 100g of the goby giant macrobenthos. Suggesting that the composition of the extract may be useful for antioxidant compositions.

Example  3: Different processing method Giant  Check vitamin E content

The content of vitamin E in the giant embryos of the present invention was analyzed under the condition of rice and roasting, which are general processing forms of rice. The results were as follows.

Example  3-1: Determining the content of vitamin E in rice

The vitamin E content of rice, which is a typical processing form of rice, was analyzed and the results are shown in Table 4 below.

Vitamin E Japan
( mg / 100g) Health Promotion
( mg / 100g) Snowball
( Giant biomass )
( mg / 100g) α- Tocopherol 0.44 + 0.12 0.95 + 0.04 2.16 ± 0.10 γ- Tocopherol 0.31 + 0.04 0.11 + 0.08 0.61 + - 0.23 α- Tocotrienol 0.03 ± 0.01 0.06 + 0.04 0.10 0.03 γ- Tocotrienol 0.08 0.02 0.13 + 0.07 0.12 + 0.04 Sum 0.86 1.25 3.00

In general rice, rice contains 0.44mg / 100g of α-tocopherol, which reduces the content to half of that of brown rice. The content of α-tocopherol is 2.16mg / 100g, which is 47% higher than that of brown rice. , 5 times more than regular rice, and more than 2 times more than healthy red rice. On the other hand, there was almost no change in the content of Health Hongmi when compared with brown rice.

In the case of γ-tocopherol, the content of 0.31 mg / 100 g was found in general rice, whereas the content of 0.61 mg / 100 g in the goblet rice goby was shown. The content of α-tocotrienol was also doubled (0.10 mg / 100g).

The content of total vitamin E was 3.00mg / 100g, which is more than 3 times as much as that of general rice (0.9mg / 100g).

Thus, it was shown that the goby giant embryos can be usefully used in the form of antioxidative food compositions, especially rice, cereal rice or retort rice.

Example  3-2: Fried brown rice  Check vitamin E content

The vitamin E content in the case of roasted brown rice, another processing type of rice, was analyzed and the results are shown in Table 5 below.

Vitamin E
Japan
( mg / 100g) Health Promotion
( mg / 100g) Snowball
( Giant biomass )
( mg / 100g) α- Tocopherol 2.00 ± 0.11 1.55 + 0.04 3.19 ± 0.13 γ- Tocopherol 0.69 0.0 0.13 + - 0.01 0.36 + 0.02 α- Tocotrienol 0.16 ± 0.01 0.14 ± 0.01 0.18 ± 0.01 γ- Tocotrienol 0.16 ± 0.01 0.16 ± 0.01 0.16 ± 0.0 Sum 2.99 1.98 3.89

As a result, the content of α-tocopherol was 2.0mg / 100g, and the content of β-tocopherol was 2.9 times higher than that of brown rice. The content of α-tocopherol was 2 times higher than that of brown rice Respectively. A similar increase was also observed in the case of roasted red pepper.

In the case of γ-tocopherol, the content of 0.69 mg / 100 g of roasted common mackerel was 0.36 mg / 100 g of roasted black mackerel giant mycelium. The content of α-tocotrienol was 0.18mg / 100g, which was 3 times higher than that of brown rice. However, there was no significant difference in the content of α-tocotrienol when compared with that of roasted rice.

The content of total vitamin E was 3.89mg / 100g, which was higher than that of 2.99mg / 100g.

The increase in the content of vitamin E in rice germinated rice bran and roasted bamboo shoots is considered to be a phenomenon in which the extraction efficiency of vitamin E is increased by heating in the cell wall of the giant embryonic stem, .

Example  4: According to processing method Hexane  Comparison of lipid-soluble extract contents

Table 6 shows the results of analyzing the content of crude extract using hexane, which is a typical organic solvent used for extracting fat-soluble components of cereals.

Liposuction Crude extract  content Japan (%) Health Promotion (%) Snowball (%)
( Giant biomass ) Brown rice 3.1 ± 0.04 2.6 ± 0.02 3.8 ± 0.06 rice 2.8 ± 0.0 2.4 ± 0.02 3.7 ± 0.29 stir-fry 2.9 ± 0.04 2.5 ± 0.01 3.7 ± 0.01

As a result, in the state of brown rice, 3.1% of lipid soluble material was extracted in general rice, and 3.8% was extracted in macroglobulin giant embryo. When rice was cooked or roasted, the average rice yield decreased by 2.8% and 2.9%, and the goby giant embryos decreased by 0.1% to 3.7%.

Example  5: Brown rice according to extraction solvent Crude extract  yield

Table 7 shows the recovery rates of crude extracts obtained by using water and alcohol for cereals.

Crude extract  content Japan (%) Health Promotion (%) Snowball (%)
( Giant biomass ) H ₂ O 6.8 3.7 10.4 40% EtOH 2.5 2.5 5.1 70% EtOH 2.3 1.9 4.0

As a result, in the case of water, the extraction amount of the goby giant macrobenthos was 10.4%, and in the case of 40% ethanol, the goby goby was 5.1% and the extraction amount of 70% ethanol was 4.0%.

Example  6: Antioxidant activity of brown rice extract according to extraction solvent

Table 8 shows the results of comparing the antioxidative activities of the extracts with water, ethanol, and ethyl acetate extraction solvents. The antioxidant activity was measured by confirming the radical scavenging ability and a typical assay, DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline- sulphonic acid) assay.

DPPH  Results (100 ppm ) Inhibition  (%) EtOAC 70% EtOH MeOH Japan 22.3 14.8 5.2 Snowball
( Giant biomass ) 43.1 23.7 37.2 ABTS  Results (100 ppm ) Inhibition (%) EtOAC 70% EtOH MeOH Japan 16.8 32.2 38.5 Snowball
( Giant biomass ) 29.5 73.8 71.4

As a result, in the case of ethyl acetate extracting the fat soluble fraction, the DPPH antioxidant activity showed 43.1% inhibitory activity, which is more than 2 times higher than that of the normal animal, and 7 times more activity than the methanol extract. The antioxidative activity of ABTS showed 2 - fold higher activity in ethyl acetate extract than in normal rice, and 2 - fold higher antioxidant activity in methanol extract.

The above results suggest that the extract of the present invention contains a large amount of vitamin E having antioxidant ability and thus the antioxidant ability of the extract is superior to that of the extract of vitamin E. The results are suggested to be able to be produced in the yield.

Example  7: Giant biomass  Determination of vitamin E content in brown rice and rice bran

In addition, in the present invention, vitamin E analysis of brown rice and rice bran was performed using a general beauty sunflower as a comparative group, which is a giant brachiocephalic goblet, which is an ophthalmic giant goblet, and the results are shown in Table 9 below.

Vitamin E Japan Snowball
( Giant biomass ) Brown rice
( mg / 100g) Rice bran
( mg / 100g) Brown rice
( mg / 100g) Rice bran
( mg / 100g) α- Tocopherol 0.88 ± 0.09 8.10 ± 1.5 1.50 0.06 14.0 ± 2.7 γ- Tocopherol 0.33 + 0.02 2.44 ± 0.3 0.46 + 0.02 1.3 ± 0.2 α- Tocotrienol 0.05 ± 0.01 0.34 + 0.03 0.06 ± 0.00 0.2 ± 0.02 γ- Tocotrienol 0.14 ± 0.01 0.65 ± 0.05 0.13 ± 0.00 0.35 + 0.04 Sum 1.40 + 0.04 11.53 + - 0.7 2.11 + 0.02 15.85 ± 1.5

As a result, as shown in Table 9, the content of tocopherol and tocotrienol in each of the brown rice of Japanese rice and the black rice giant macrobenthos was analyzed. As a result, the most active α-tocopherol among tocopherols was 0.88 mg / 100 g in the case of Japanese rice brown rice , Whereas the goby giant embryonic rice brown rice showed the result of containing 1.5 mg / 100 g of? -Tocopherol, which is 70% in content.

Further, in the case of? -Tocopherol, the results showed that the I. brown rice contained 0.33 mg / 100 g? -Tocopherol, while the macrophyllous giant embryo contained 0.46 mg / 100 g? -Tocopherol. On the other hand, the content of tocotrienol showed similar results in both α and γ. Total vitamin E content was found to be more than 50% higher than that of 1.4mg / 100g of Ilmi brown rice.

In addition, the content of α-tocopherol in rice bran increased by 10 times than that of brown rice in both Japanese and Chinese black ginseng, and the contents of γ-tocopherol, α-tocotrienol and γ-tocotrienol were greatly increased.

In particular, the content of α-tocopherol was higher than that of rice rice, and the content of vitamin E was higher than that of rice bran rice bran rice, .

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are illustrative in all aspects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the spirit or scope of the present invention as defined by the appended claims.

Claims (10)

A method for producing vitamin E, which comprises extracting a macroglobulin giant embryo with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, hexane and ethyl acetate.
The method according to claim 1, wherein the method further comprises separating and purifying vitamin E from the goblet extract.
The method according to claim 1, wherein the vitamin E is tocopherol or tocotrienol.
The manufacturing method according to claim 1, wherein the giant-ray-giant embryonic biceps is a blinking eye (Milyang 263).
[Claim 2] The method according to claim 1, wherein the extract is extracted from at least one selected from the group consisting of goby giant embryonic rice rice, brown rice, fried brown rice and rice bran.
The process according to claim 1, wherein the alcohol is selected from the group consisting of methanol, ethanol and butanol.
A food composition enriched with vitamin E, which comprises an extract of giant macroalbumin as an active ingredient.
8. The composition according to claim 7, wherein said giant-ray-giant embryonic stem is a blinking eye (Milyang No. 263).
A food composition enriched with vitamin E, wherein the food composition contains a large amount of biochemical gypsum as an active ingredient.
10. The method of claim 9, wherein the composition is selected from the group consisting of cereal rice, retort brown rice, bread, sikhye, meat, sausage, bread, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, Tea, a drink, an alcoholic beverage, and a vitamin complex.

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