KR20120054298A - Composition of skin external application containing fermented soybean leaves extract - Google Patents

Abstract

The present invention relates to an external composition for skin containing fermented soybean leaf extract as an active ingredient, and more particularly, by containing fermented soybean leaf extract as an active ingredient, to promote collagen production in the skin, to inhibit MMP-1, and at the same time an antioxidant effect And it relates to an external composition for skin having a skin aging inhibitory effect and wrinkle improvement through the DNA damage protection effect.

Description

Composition for skin external application containing fermented soybean leaves extract}

The present invention relates to an external composition for skin containing fermented soybean leaf extract as an active ingredient, and more particularly, by containing fermented soybean leaf extract as an active ingredient, to promote collagen production in the skin, to inhibit MMP-1, and at the same time an antioxidant effect And it relates to an external composition for skin having the effect of inhibiting skin aging and wrinkle improvement through the DNA damage protection effect.

The soybean leaves are the leaves of the annual plant belonging to the rosewood legume of Glycine max (L.) Merr. Bean leaves are commonly used in Gyeongsang-do in the form of bean leaf pickles and bean leaf kimchi. In addition, herbal medicine is used to treat snake bites or gonorrhea with bloody urine. Soybean leaves are known to have pharmacological effects such as preventing or treating diabetes, preventing gout, delaying or treating diabetes, and anti-arteriosclerosis. Or is not described.

Accordingly, the present inventors have been researching to prepare a skin external preparation which is safe for skin and has excellent cell proliferation and cell regeneration effect and excellent stability as a product, and found that fermented soybean leaf extract has very excellent cell proliferation and cell regeneration effect. The invention was completed.

Accordingly, it is an object of the present invention to provide an external composition for skin excellent in cell proliferation and cell regeneration efficacy.

In order to achieve the above object, the present invention provides a skin external preparation composition containing a fermented soybean leaf extract as an active ingredient.

The external preparation composition for skin according to the present invention has excellent antioxidant and anti-aging effects by containing fermented soybean leaf extract as an active ingredient.

The present invention relates to an external composition for skin containing fermented soybean leaf extract as an active ingredient. The external preparation composition for skin according to the present invention preferably contains 0.001 to 30% by weight of the fermented soybean leaf extract based on the total weight of the composition. If the content is less than 0.0001% by weight can not obtain the effect of cell proliferation and cell regeneration by the extract, etc., if the content is more than 30% by weight is inefficient because the increase of the effect is not large compared to the increase in content.

Fermented soybean leaf extract used as an active ingredient in the present invention is prepared by fermenting soybean leaf or its extract in order to increase the content of the active ingredient in soybean leaf extract.

In one embodiment of the present invention, the fermented soybean leaf extract is prepared by natural fermentation, homogeneous fermentation or salt fermentation, followed by adding water or ethanol, or a mixture thereof for 4 to 24 hours at 10? 90 ° C. can do.

In addition, the fermented soybean leaf extract in one embodiment of the present invention extracts soybean leaf with water or ethanol, or a mixture thereof for 10 to 90 ℃ for 4 to 24 hours by a method well known in the art and then the extract is natural fermentation, bacteria It can be prepared by fermentation or salt fermentation.

In an embodiment of the present invention, the soybean leaves or extracts thereof are put in a container such as Onggi and placed in a shade, and then fermented through a process of fermentation for 1 to 1 year, preferably 1 to 30 days, without artificial manipulation in a natural environment. Can be.

In addition, in one embodiment of the present invention inoculated useful bean strains known in the art such as yeast, mushroom, lactic acid, yeast, or Bacillus genus (battery) to the soybean leaves or extracts thereof for 18 hours to 100 days at 10 ~ 60 ℃ After the degree of fermentation, it can be fermented.

Yeast bacteria used in the present invention is Saccharomyces sp . , Ski investigation caromyces ( Schizosaccharomyces sp . ), Torulopsis sp . Rhodotorula sp . ) Or Candida sp . The fungus can be any mushroom that is edible, but preferably Chaga ( Inonnotus - obliquus ), Cordyceps fungus ( Cordyceps) sinensis , Paecilomyces japonica ), matsutake mushroom ( Tricholomamatsutake ), situation mushroom ( Phellinus) linteus ) or Ganoderma lucidum ( Ganoderma) lucidum ) and the like. In addition, Lactobacillus ( Lactobacillus) sp . ), Streptococcus sp . ), Leuconostoc sp . ) Or Bifidobacteria sp . ) And the like, is nurukgyun rayijo crispus (Rhyzopus), Usami (Usami) and duck material (Oryzae), Aspergillus duck material (Aspergillus oryzae ) or Aspergillus sojae ) and the like, Bacillus genus (Bacillus subtilis) Bacillus licheniformis ( Bacillus licheniformis ) F1017, Bacillus licheniformis ( Bacillus licheniformis) F1232, Bacillus piece you miss PORTO (Bacillus licheniformis) F1267, Bacillus piece you miss PORTO (Bacillus licheniformis) F1268, Bacillus piece you miss PORTO (Bacillus licheniformis) F1200, Bacillus pumi Ruth (Bacillus pumilus) F1337, Bacillus Sono alkylene sheath (Bacillus sonorensis ) F1005, Bacillus subtilis subtilis ) F1236 or Bacillus subtilis ) F1279, etc., but is not limited thereto.

When fermentation using yeast in one embodiment of the present invention, inoculated yeast at a constant concentration (about 10 ⁵ to 10 ⁷ cell / mL) in the basal medium containing soybean leaves or its extract, pH 5.5 ~ 6.5, temperature After culturing while maintaining the conditions at 25-50 ° C. and aeration of 0.05-0.5 VVM, the fermented soybean leaf extract can be obtained by centrifuging the fermentation broth to remove the polymer precipitate and yeast. Incubation time is preferably about 24 to 120 hours.

In addition, in one embodiment of the present invention, when fermented with mushrooms, inoculate mushrooms to soybean leaves or extracts thereof, and then cultured while maintaining the conditions of temperature 18 ~ 25 ℃, humidity 60 ~ 80%, water Or anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform, 1,3-butylene glycol, hexane Alternatively, by using at least one of diethyl ether, and heated under 50 ~ 100 ℃, 1-5 hours can be concentrated under reduced pressure to obtain a fermented soybean leaf extract. Incubation time is preferably about 1 to 100 days.

In addition, when the fermentation using lactic acid bacteria in one embodiment of the present invention, by inoculating the lactic acid bacteria at a constant concentration (about 10 ⁵ to 10 ⁷ cell / mL) in the basal medium containing soybean leaves or its extract, pH 6.5 ~ 7.5 After culturing while maintaining the temperature of 25-50 ° C. and aeration rate of 1 VVM, the fermentation broth extract may be obtained by centrifugation of the fermentation broth to remove the polymer precipitate and the lactic acid bacteria. Incubation time is preferably about 24 to 120 hours.

In addition, when fermentation using Nuruk bacteria in one embodiment of the present invention, inoculated with koji bacteria on soybean leaves or extracts thereof, followed by culturing while maintaining the temperature of 15 ~ 45 ℃, centrifugation of the fermentation broth and polymer precipitates and Fermented soybean leaf extract from which yeast is removed can be obtained. The culture time is preferably about 18 to 120 hours.

In addition, when fermented using Bacillus genus in one embodiment of the present invention, Bacillus genus inoculated at a constant concentration (about 10 ⁵ to 10 ⁷ cell / mL) in the basal medium containing soybean leaves or its extract, temperature 10 After culturing while maintaining the condition of -60 ℃, this fermentation broth can be centrifuged to obtain a fermented soybean leaf extract from which pH 5.5-8.5, polymer precipitates and Bacillus bacteria are removed. Incubation time is preferably about 24 to 96 hours.

In addition, in one embodiment of the present invention 1) adding a certain amount of salt to the soybean leaves or extracts of the long-term aging fermentation; And 2) extracting the salted fermented product with water or an organic solvent to obtain a fermented extract.

In the step 1), the salt used for salting is preferably purified high purity sodium chloride, sun salt, rock salt and bamboo salt, and the concentration of salt is preferably 10 to 30% by weight based on the total fermented product. When the content of salt is less than 10% by weight, it is difficult to achieve the expected effect, and when it exceeds 30% by weight, the effect is not changed.

The fermentation process of 1) is between 4 to 40 ℃, so that enough fermentation can occur from 1 day to 1 year, preferably 1 to 30 days is good to ferment. If it is less than 4 ℃, the growth of useful microorganisms is not well, and if it exceeds 40 ℃ can grow too much microbial may affect the performance of the soybean leaves or its extract.

The extraction solvent used in step 2) may be used alone or in combination of two or more selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane. Can be. At this time, the extraction temperature is preferably 10 to 80 ℃, it can be extracted for 6 hours to 24 hours. If the extraction temperature and extraction time is out of the extraction efficiency may decrease or change of components may occur.

In the present invention, the salt or fermented extract of soybean leaves may be prepared by evaporation, spray drying or lyophilization of the liquid obtained by cooling, heating and filtration at room temperature in a conventional method known in the art after step 2). .

Fermented soybean leaf extract according to the present invention is not particularly limited to its use, for example, it can be used in pharmaceutical compositions and the like in addition to the external preparation composition for the skin.

In addition, the topical skin composition according to the present invention may further contain a cosmetically or dermatologically acceptable medium or base commonly used in the art. These are all formulations suitable for topical application according to conventional methods in the art, for example emulsions, suspensions, microemulsions, microcapsules, microgranules, obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases. Alternatively, they may be prepared in the form of ionic (liposomal) and nonionic vesicle dispersants, or in the form of creams, skins, lotions, powders, ointments, sprays, or cone sticks. The external preparation composition for skin according to the present invention may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the composition for external application for skin according to the present invention may be a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type or Nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics It may further contain an adjuvant commonly used in the cosmetic or dermatological field, such as may be introduced in an amount generally used in the cosmetic or dermatological field.

In addition, the topical skin composition according to the present invention is not particularly limited in the formulation, for example, softening longevity, astringent longevity, nourishing longevity, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing It may be formulated into a cosmetic such as foam, cleansing water, pack, powder, body lotion, body cream, body oil or body essence.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. These examples are provided only for understanding the contents of the present invention, but the scope of the present invention is not limited to these examples and test examples, and modifications, substitutions, and insertions commonly known in the art may be performed. It may be included in the scope of the present invention.

Example 1 Preparation of Fermented Soybean Leaf Extract

1.5 kg of soybean leaves were put in 2 L of water and boiled at medium heat (60-80 ° C) for 1 hour. The boiled bean leaves were taken out and cooled down at room temperature for 1 hour, and the cooled bean leaves were placed in Onggi for 7 days at room temperature. After crushing the soybean leaves with a blender and put 7 L of 80% ethanol aqueous solution, three times reflux extraction, and then was deposited at 15 ℃ for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 357 g of the fermented soybean leaf extract.

Comparative Example 1 Preparation of Soybean Leaf Extract

1.5 kg of soybean leaves were pulverized with a blender, 7 L of an 80% ethanol aqueous solution was added thereto, and refluxed three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 357 g of a soybean leaf extract.

Example 2 Fermented Soybean Leaf Extract Using Yeast

The soybean leaf extract of Comparative Example 1 was added to 1.5 L of purified water so that 5 wt% was used as the basic composition of carbon source, nitrogen source, phosphate, inorganic salts and micronutrient components, and using sodium hydroxide to pH 5.5-6.5 appropriate. Fit. Saccharomyces in the culture medium containing the soybean leaf extract ( Saccharomyces sp . ) Was inoculated at 10 ⁶ cells / mL, and cultured for 72 hours while maintaining the conditions of pH 5.5-6.5, temperature 25-35 ° C, and aeration 0.5 VVM.

The fermentation broth was centrifuged at 1.000xg for 30 minutes to obtain a final fermented soybean leaf extract from which polymer precipitates and yeast cells were removed.

Example 3 Fermented Soybean Leaf Extract Using Mushroom Bacteria

Cordyceps fungi ( Paecilomyces) on the soybean leaf extract of Comparative Example 1 japonica ) was added at 5% by weight, fermented at 18-25 ° C. and humidity 60-70% for 60 days, dried, and then ground. Boiled in reflux extractor for 3 hours and extracted. Then filtered through 100 mesh filter cloth and the residue was extracted once more in the same way. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain a final fermented soybean leaf extract.

Example 4 Fermented Soybean Leaf Extract Using Lactic Acid Bacteria

The soybean leaf extract of Comparative Example 1 was added to 1.5 L of purified water so as to be 5% by weight, and the carbon composition, nitrogen source, phosphate, inorganic salts, and micronutrient components were used as basic compositions, and sodium hydroxide was used at an appropriate pH of 5.5 to 6.5. Fit. Lactobacillus in the culture medium containing the soybean leaf extract sp . ) Was inoculated at 10 ⁶ cells / mL, and cultured for 72 hours while maintaining the conditions of pH 6.5-7.5, temperature 35-38 ° C, and aeration volume 1 VVM.

The fermentation broth was centrifuged at 1.000xg for 30 minutes to obtain a final fermented soybean leaf extract from which polymer precipitates and lactic acid bacteria cells were removed.

Example 5 Fermented Soybean Leaf Extract Using Nuruk

5% by weight of Aspergillus oryza ( Aspergillus oryzae ) was added to the soybean leaf extract of Comparative Example 1, fermented at 25 ° C. for 48 hours, dried, and then ground. Each was put in 3 l and extracted by boiling for 3 hours in a reflux extractor with a cooling capacitor. Then filtered through 100 mesh filter cloth and the residue was extracted once more in the same way. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain a final fermented soybean leaf extract.

Example 6 Fermented Soybean Leaf Extract Using Bacillus

The soybean leaf extract of Comparative Example 1 was added to 1.5 L of purified water so that 5 wt% was used as the basic composition of carbon source, nitrogen source, phosphate, inorganic salts and micronutrient components, and using sodium hydroxide to pH 5.5-6.5 appropriate. Fit. Bacillus subtilis ( Bacillus) in the culture medium containing the soybean leaf extract subtilis ) F1279 was inoculated and incubated at 45 ° C. for 72 hours.

The fermentation broth was centrifuged at 1.000xg for 30 minutes to obtain a final fermented soybean leaf extract from which polymer precipitates and Bacillus cells were removed.

Example 7 Preparation of Soybean Leaf Salted Fermented Extract

After washing 1 kg of soybean leaves with clean water and drying it, mix it with a salt solution of 10 wt% concentration, prepare it in Onggi, ferment at about 4 ℃ for 30 days, add 5 L of 80% ethanol aqueous solution, and reflux 3 times. Next, it was immersed at 15 degreeC for 1 day. Thereafter, the residue and the filtrate were separated by filter cloth filtration and centrifugation, and the extract obtained by concentrating the separated filtrate under reduced pressure was suspended in water, extracted five times with 1 L of ether to remove the pigment, and the aqueous layer was 1-butanol. Extracted three times with 500 mL. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract, which was dissolved in a small amount of methanol, and then added to a large amount of ethyl acetate, followed by drying the resulting precipitate. Obtained.

Test Example 1 Antioxidant Effect: DPPH Test

In order to examine the antioxidant effects of the extracts prepared in Examples 1-7 and Comparative Example 1, the organic radical DPPH (1,1-diphenyl-2-picrylhydrazyl; 1,1-diphenyl-2-picryl It was performed by evaluating the antioxidant capacity by comparing the DPPH oxidation inhibition effect by the change in absorbance generated by the reduction of hydrazyl) (antioxidant is oxidized). That is, the concentration of the extracts prepared in Examples 1 to 7 and Comparative Example 1 was inhibited by the inhibition of DPPH and the degree of absorbance was reduced compared to the control group, and the concentration exhibiting absorbance of 50% or less than the absorbance of the control group was measured. Evaluated as effective antioxidant concentration.

190 μl of 100 μM (dissolved in ethanol) DPPH solution and 10 μl each of Comparative Example 1, Example 1-7 and the positive control group obtained above were added to make a reaction solution, and reacted at 37 ° C. for 30 minutes and then absorbance at 540 nm. Was measured. As the positive control group, Trolox, a synthetic antioxidant widely used, was used. The results of DPPH analysis of the respective substances are shown in Table 1 below, and IC ₅₀ means the sample concentration when the absorbance was reduced by 50% by the added sample.

DPPH analysis results Test substance IC ₅₀ (ppm) Trolox 45 Comparative Example 1 66 Example 1 19 Example 2 26 Example 3 30 Example 4 23 Example 5 20 Example 6 28 Example 7 27

In the results of Table 1, the antioxidant capacity of the fermented soybean leaf extract of Examples 1 to 7 is not only superior to the raw material extract (Comparative Example 1), but also its effect is excellent even when compared to trolox, a synthetic antioxidant used as a positive control group. It confirmed that it was excellent.

Therefore, the fermented soybean leaf extract according to the present invention was found to have an excellent antioxidant effect.

Test Example 2 Anti-aging Effect: Inhibition of Collagenase Expression

The collagenase production inhibitory ability of the extracts prepared in Examples 1-7 and Comparative Example 1 was measured.

Human normal fibroblasts were cultured in a 12 well plate incubator at a concentration of 10 ⁵ and then the extracts of Comparative Examples 1 and 7 were replaced with a medium containing the concentrations of 1, 10 and 50 ppm, respectively. As a positive control, TGF-β (Human Transforming Growth Factor-β1, Roche Co.) was used at a concentration of 10 ng / ml, and Comparative Example 1, Examples 1-7 and TGF-β were not included. As a negative control. Cells were harvested on the third day of culture to quantify the amount of type I procollagen produced by ELISA. The amount of procollagen of the negative control group was set to 0 and the amount of procollagen of the positive control group was set to 100, and the comparison with each measured value was calculated. The results are shown in Table 2 below.

Test substance Type I procollagen biosynthesis (%) Negative control group 0 Example 1 1 ppm 33.7 10 ppm 41.8 50 ppm 42.5 Example 2 1 ppm 36.8 10 ppm 45.6 50 ppm 49.3 Example 3 1 ppm 40.8 10 ppm 50.5 50 ppm 54.6 Example 4 1 ppm 43.1 10 ppm 53.4 50 ppm 57.6 Example 5 1 ppm 39.1 10 ppm 48.4 50 ppm 52.3 Example 6 1 ppm 42.0 10 ppm 52.1 50 ppm 56.2 Example 7 1 ppm 41.4 10 ppm 51.3 50 ppm 55.4 Comparative Example 1 1 ppm 25.9 10 ppm 30.9 50 ppm 35.7 TGF-β (10 ng / ml) 100

In the results of Table 2, the fermented soybean leaf extracts of Examples 1-7 in the normal human fibroblast monolayer culture system promoted type I procollagen production in a concentration-dependent manner compared to the negative control. In addition, the fermented soybean leaf extract of Examples 1 to 7 according to the present invention was superior to the type I procollagen production effect than the general soybean leaf extract of Comparative Example 1.

Therefore, the fermented soybean leaf extract of the present invention was confirmed that by promoting collagen production to alleviate the reduction of collagen caused by aging of the human skin can provide an excellent anti-aging effect.

Test Example 3 Anti-aging Effect: Inhibition of MMP-1 Expression

Human fibroblasts were incubated in a 12-well plate incubator at a concentration of 10 ⁵ and then irradiated with ultraviolet B at 40 mJ / cm 2, and then the medium prepared in Examples 1 to 7 and Comparative Example 1 was used in a medium containing 1 and 10 ppm, respectively. Replaced. The cells were harvested on the second day of culture (harvest) to quantify the amount of MMP-1 (matrix metalloproteinase I) produced by ELISA. The result was calculated as a comparison with the value measured by using a UV control group containing no test substance 100, the positive control group was used Retinoic acid (RA, Sigma. USA).

Test substance MMP-1 expression level Control group 100 Retinoic acid 10 μM 45 Example 1 1 ppm 52 10 ppm 46 Example 2 1 ppm 57 10 ppm 47 Example 3 1 ppm 62 10 ppm 51 Example 4 1 ppm 59 10 ppm 46 Example 5 1 ppm 63 10 ppm 50 Example 6 1 ppm 59 10 ppm 51 Example 7 1 ppm 62 10 ppm 49 Comparative Example 1 1 ppm 113 10 ppm 82

In the results of Table 3, the fermented soybean leaf extract of Examples 1 to 7 according to the present invention in the human normal fibroblast monolayer culture system significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / cm 2. Therefore, the fermented soybean leaf extract of Examples 1 to 7 according to the present invention inhibits the biosynthesis of skin tissue degrading enzyme MMP-1 generated by internal aging or external environmental factors, thereby inhibiting skin aging and improving wrinkles. It was confirmed.

Test Example 3 Anti-aging Effect: Inhibitory Activity against DNA Oxidative Damage by Single Cell Gel Electrophoresis (SCGE)

After culturing human fibroblasts for 24 hours, the extracts prepared in Examples 1-7 and Comparative Example 1 were treated with respective concentrations, and after 12 hours, the positive control group H ₂ O ₂ (final concentration 10 ^−3). M) treatment and staining by electrophoresis were observed by fluorescence microscope. At this time, the length (μm) of the tail (tail) as a result of DNA cleavage in each cell was analyzed using KOMET 3.1 (Kinetic Imaging, England), an image analyzer, and the results are shown in Table 4 below.

Inhibition of DNA Oxidative Damage Test substance Tail length (μm) Inhibition rate (%) H ₂ O ₂ 115.6 - H ₂ O ₂ + Example 1 10 ppm 71.3 38.3 50 ppm 64.5 44.2 100 ppm 58.5 49.4 H ₂ O ₂ + Example 2 10 ppm 72.8 37.0 50 ppm 72.0 37.7 100 ppm 45.6 60.6 H ₂ O ₂ + Example 3 10 ppm 70.6 38.9 50 ppm 61.8 46.6 100 ppm 54.6 52.7 H ₂ O ₂ + Example 4 10 ppm 76.4 33.9 50 ppm 71.4 38.2 100 ppm 42.9 62.9 H ₂ O ₂ + Example 5 10 ppm 80.6 30.2 50 ppm 77.7 32.8 100 ppm 51.9 55.1 H ₂ O ₂ + Example 6 10 ppm 74.7 35.3 50 ppm 73.1 36.7 100 ppm 64.7 44.0 H ₂ O ₂ + Example 7 10 ppm 72.9 36.9 50 ppm 62.9 45.6 100 ppm 53.5 53.7 H ₂ O ₂ + Comparative Example 1 10 ppm 105.6 8.6 50 ppm 103.4 10.5 100 ppm 89.9 22.3 H ₂ O ₂ + Vitamin E 10 ppm 70.6 38.9

In the results of Table 4, the fermented soybean leaf extract of Examples 1-7 showed significant oxidative DNA damage inhibitory activity, whereby the fermented soybean leaf extract according to the present invention is a DNA single by hydroxyl radical (.OH) It was confirmed that it acts as a DNA oxidative damage inhibitor that inhibits the strand strand break.

Claims (4)

Skin external composition containing fermented soybean leaf extract as an active ingredient. The composition for external application for skin according to claim 1, wherein the fermented soybean leaf extract is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. According to claim 1, wherein the fermented soybean leaf extract is a composition for external application to the skin, characterized in that it is prepared by natural fermentation, homogeneous fermentation or salt fermentation. The composition for external application for skin according to claim 3, wherein the soybean leaf extract is prepared by adding water or ethanol or a mixture thereof to the soybean leaf for 4 to 24 hours at 10 to 90 ° C.