KR20120034270A - Composition of beauty foods for preventing wrinkles caused by photoaging - Google Patents

Abstract

The present invention relates to a cosmetic food composition having an effect of preventing and improving skin wrinkles generated by photoaging due to ultraviolet rays. More specifically, it contains two or more of star fruits leaf extracts, Ginsenoside Rb1 fortified fractions or seaweed pine peel extract, and the effect of preventing skin wrinkles and elasticity decrease due to photoaging due to ultraviolet light It relates to an oral cosmetic composition having a.

Description

Beauty food composition for preventing skin wrinkles due to photoaging {COMPOSITION OF BEAUTY FOODS FOR PREVENTING WRINKLES CAUSED BY PHOTOAGING}

The present invention relates to a cosmetic food composition having an effect of preventing and improving skin wrinkles generated by photoaging due to ultraviolet rays. More specifically, it contains two or more of star fruits leaf extracts, Ginsenoside Rb1 fortified fractions or seaweed pine peel extract, and the effect of preventing skin wrinkles and elasticity decrease due to photoaging due to ultraviolet light It relates to an oral cosmetic composition having a.

Skin is composed of several layers such as epidermis, dermis and subcutaneous fat. The epidermal layer forming the uppermost layer of the skin can be divided into stratum corneum, granule layer, polar layer and basal layer. The stratum corneum prevents water evaporation of the skin and prevents penetration of foreign substances. The skin needs to be supplied with oxygen, moisture and nutrients through the blood so that it can shine and maintain its elasticity.

The most important function of the skin is the barrier function that protects the internal organs from the external environment and prevents water loss. The most important environmental factor affecting the skin is ultraviolet (UV). Since the skin is exposed to sunlight, it is always exposed to ultraviolet light. The aging caused by ultraviolet rays is called photoaging, and excessive ultraviolet rays cause burns or pigmentation on the skin, and when exposed for a long time, they promote appearance changes such as skin roughness, loss of elasticity, and wrinkles. It can also cause diseases like skin cancer.

Ultraviolet rays cause reactive oxygen species in skin cells, which in turn alter cell constituents and cause loss of function, induce the production of collagen degrading enzymes, and collagen and elastic fibers. It breaks down and causes wrinkles. Therefore, in order to suppress photoaging caused by ultraviolet rays, it is necessary to promote collagen production, inhibit degrading enzyme activity, and to remove free radicals generated by ultraviolet rays.

Previously, it has relied on cosmetics or external preparations applied to the skin surface to prevent skin aging. However, since it does not absorb up to the dermal layer and acts only on the epidermis, the effect on skin aging is temporary, and because it does not remove the active oxygen in the body, there is a limit to prevent the aging effect. Accordingly, the development of beauty foods that can suppress and prevent skin aging symptoms caused by ultraviolet rays by ingestion for oral use has been attempted.

Therefore, the present inventors studied to find a composition exhibiting a prophylactic effect on skin aging due to photoaging, as a result, starfruit leaf extract, ginsenoside Rb1 enhanced fraction, which is known to have an active oxygen scavenging ability, an increase in collagen synthesis, and an inhibitory activity of collagen degrading enzyme, and When ingesting a complex containing two or more of the sea the pine bark extract, it was found that there is an effect such as inhibiting skin wrinkle formation and elasticity lowering, preventing skin moisture decrease decrease without the side effects. In addition, when two or more of the above materials are combined, the present invention has been found to have a remarkable effect on the anti-wrinkle effect than a single material, which is unpredictable to those skilled in the art.

Accordingly, an object of the present invention is to prevent skin wrinkles and elasticity degradation due to photoaging caused by ultraviolet light and to reduce the skin moisture decrease, two or more of the star fruit leaf extract, ginsenoside Rb1 enhanced fraction and seaweed pine peel extract It is to provide a composition containing.

It is also an object of the present invention to provide a beauty food containing the beauty food composition.

In order to achieve the above object, the present invention provides an oral cosmetic composition containing 70 to 90% by weight of the star fruit leaf extract and 10 to 30% by weight of the sea berry peel extract based on the total weight of the composition.

The present invention also provides an oral cosmetic composition containing 65 to 85% by weight of Starfruit leaf extract and 15 to 35% by weight of ginsenoside Rb1 fortified fraction, based on the total weight of the composition.

The present invention also provides an oral cosmetic composition containing 25 to 45% by weight of the seaside pine extract and 55 to 75% by weight of the ginsenoside Rb1 enriched fraction, based on the total weight of the composition.

The present invention also provides an oral cosmetic composition containing 50 to 60% by weight of starfruit leaf extract, 20 to 30% by weight of ginsenoside Rb1 fortified fraction, and 10 to 20% by weight of seaweed pine peel extract. to provide.

The present invention also provides a beauty food containing the beauty food composition.

The cosmetic food composition according to the present invention contains starfruit leaf extract, ginsenoside Rb1 enhanced fraction or seaweed pine peel extract in a specific range of ratios, thereby preventing skin aging such as skin wrinkles and elasticity that may be caused by ultraviolet rays. It has the effect of preventing and improving, and it is preferable because it is made of natural substances and has almost no side effects.

1 is a result of analyzing the wrinkle depth of the skin wrinkle skin replica prepared from the mouse skin 10 weeks after ingestion of the test material using a skin visiometer.
**) The index of R indicates the depth of wrinkles. The larger the number of R values, the deeper the wrinkles.
Figure 2 is a photograph prepared by comparing the wrinkle replica produced in the mouse skin 10 weeks after the intake of the test substance (normal control, UV irradiation positive control, composition A-D ingestion group, respectively).

The present invention provides an oral cosmetic composition containing two or more of starfruit leaf extract, ginsenoside Rb1 enriched fraction or seaweed pine peel extract.

The starfruit leaf extract used in the present invention (carambola leaf extract) is characterized in that it contains an extract from the leaves of the evergreen tree (Averrhoa carambola L.) of the hoebab as an active ingredient, the carram which is a specific flavonoid (flavonoid) It is characterized by containing carambola flavones. Starfruit Leaf Extract (JP-A-2002-226323) has collagen production, collagenase activity inhibition, fibroblast proliferation and elastase inhibitory activity to prevent and improve skin aging. Known as a useful ingredient. In addition, the skin epidermal hyaluronic acid synthase 3mRNA expression promotion, aquaporin 3mRNA expression promotion, Serine palmitoyl trasferase mRNA expression promoters are known (Japanese Patent Publication No. 2009-191039) and skin aging symptoms and It has been studied to have an effect on skin rehydration.

Maritime pine bark extract (maritime pine bark extract) used in the present invention is extracted and purified from the pine bark extract of the coast of France with water or alcohol, also called trade names such as Pycnogenol or Flavangenol. . This extract has high antioxidant power and inhibits the activity of matrix metalloproteinase-1 (MMP-1), a collagen degrading enzyme, and has been known to have skin elasticity and whitening effect when taken alone or in combination with other substances (Z.Ni et. al . , Phytotherapy research 16: 567-571, 2002; D. Segger et al . , Journal of Dermatological Treatment 15: 222-226, 2004).

The ginsenoside Rb1 fortified fraction used in the present invention is an extract fraction prepared in Korean Patent Application Publication (Korean Patent Publication No. 10-2010-0049975), and concentrated by extracting the ginseng spirit extract and then adsorbed by diluting the extract with water to the adsorption resin. And ginsenoside Rb1-enriched extract fractions, comprising the step of eluting the non-adsorbed components by adding 50-80% v / v% alcohol. Ginsenoside Rb1 is known to have an anti-wrinkle effect because of its ability to increase collagen biosynthesis in human fibroblasts and to inhibit MMP-1 (Matrix metalloproteinase-1) activity, a collagen degrading enzyme (Kim Nami et al. 31 (2): 86-92, 2007).

In one embodiment, the present invention provides an oral cosmetic composition containing a starfruit leaf extract and sea berry peel extract. Preferably, the composition comprises 70 to 90% by weight starfruit leaf extract and 10 to 30% by weight seaside pine extract, based on the total weight of the composition.

In one embodiment, the present invention also provides an oral cosmetic composition containing a starfruit leaf extract and a ginsenoside Rb1 enriched fraction. Preferably, the composition comprises 65 to 85% by weight starfruit leaf extract and 15 to 35% by weight ginsenoside Rb1 enriched fraction, relative to the total weight of the composition.

When the star fruit leaf extract is mixed in a large proportion of more than 90% by weight when mixed with the sea berry peel extract or the ginsenoside Rb1 enriched fraction, it does not show the effect as the composition combined in the above ratio. In addition, it is difficult to expect the anti-wrinkle effect when the sea pine peel extract or ginsenoside Rb1 enhanced fractions are mixed with the starfruit leaf extract at 10 to 15% by weight or less, respectively.

In one embodiment, the present invention also provides an oral cosmetic composition containing sea berry bark extract and ginsenoside Rb1 enriched fraction. Preferably, the composition comprises from 25 to 45% by weight of Seaside Peel Extract and 45 to 75% by weight of ginsenoside Rb1 enriched fraction, relative to the total weight of the composition.

As can be seen from the results of Experimental Example 1, when ingestion of a single extract of the haeko pine bark extract or ginsenoside Rb1 enriched fraction, it was confirmed that the degree of inhibition of wrinkle formation of the ginsenoside Rb1 enriched fraction appeared to be slightly higher, but showed a significance The effect was not good enough. In addition, when the seacoast bark extract and the ginsenoside Rb1 reinforced fractions are mixed, the anti-wrinkle effect is higher than that of the single substance as in Experimental Example 2. When seacoast bark extract is mixed in less than 25% by weight it is difficult to expect the effect of inhibiting wrinkle formation.

In a most preferred embodiment, the present invention provides an oral cosmetic composition containing a starfruit fruit extract, a ginsenoside Rb1 enriched fraction, and a seaweed peel extract. Preferably the composition comprises from 10 to 60% by weight of Starfruit leaf extract, from 5 to 50% by weight of seaside peel extract and from 5 to 50% by weight of ginsenoside Rb1 fortified fraction, based on the total weight of the composition.

The cosmetic food composition according to the present invention is applicable to the food of various formulations, such as tablets, capsules, pills, granules, drinks.

Hereinafter, the contents of the present invention will be described in more detail with reference to Examples. The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

EXAMPLE

Example  One : Star fruit  Preparation of Leaf Extract

Soluble components were extracted for 1 to 3 hours while stirring using a starfruit dried leaf and 5-15 times the alcohol of the raw material. The extract was filtered to remove the residue, and only the extract was concentrated under reduced pressure. The mixture was combined with dextrin, heat sterilized, spray dried, and powdered to be used in the experiment.

Example  2 : Gin Senocide Rb1  reinforce Fraction  Produce

70% alcohol was added to the dried four-year-old rice ginseng by 15 times the weight of the sample, and extracted three times at 24 ° C. for 24 hours. The alcohol extract was heated and concentrated to 80 brix (Bx), diluted with 5 times the weight of water, and adsorbed to the saponin component by passing through the adsorbent resin Diion HP-20 resin. A fraction enriched with ginsenoside Rb1 was prepared by eluting with 50-80% (v / v) alcohol and used for the experiment.

Example  3: Seaside song  Preparation of Bark Extract

The pine bark of the coast of France was crushed and extracted with alcohol or water. The extract was concentrated under reduced pressure drying, the total procyanidin content was adjusted through purification using a column, and then spray-dried to powderize and used for the experiment.

Example  4: Preparation of Composition A

The starfruit leaf extract prepared in accordance with Examples 1 and 3 and the extract from the seacoast peel were mixed in a weight ratio of 75.0: 25.0.

Example  5: Preparation of Composition B

The starfruit leaf extract and ginsenoside Rb1 fortified fractions prepared according to Examples 1 and 2 were mixed to a weight ratio of 75.0: 25.0.

Example  6: Preparation of Composition C

Seacoast bark extract prepared according to Examples 2 and 3 and ginsenoside Rb1 enhanced fractions were mixed to a weight ratio of 35.0: 65.0.

Example  7: Preparation of Composition D

Each of the starfruit leaf extract, ginsenoside Rb1 enriched fraction, and coastal pine shell extract prepared according to Examples 1, 2, and 3 was mixed so that the weight ratio was 50.0: 30.0: 20.0.

Example  8: Procollagen production amount measurement

In order to compare the procollagen production capacity of each test substance and composition, 1.6 × 10 ⁵ cells / mL of fibroblasts (fibroblast NB1RGB) were attached to a 96-well plate.

Thereafter, the wells were treated with starfruit leaf extract, sea berry bark extract, and ginsenoside Rb1 enriched fraction, respectively, by concentration. After 72 hours of incubation, the amount of procollagen production in the medium of each well was calculated by ELISA. At this time, the control group was not added to a separate sample as 100 and the comparison value is shown in Table 1 below.

Procollagen Production division Concentration (μg / mL) Procollagen Production (%)
Starfruit Leaf Extract 6.25 106 25 115 100 120
Coastal Pine Bark Extract 6.25 108 25 43 100 -
Ginsenoside Rb1 fortified fraction 6.25 109 25 122 100 99.8

As can be seen in Table 1, in the case of the Starfruit leaf extract, procollagen production increased with increasing concentration. However, in the case of coastal bark extract, the amount of collagen production decreased or not produced at all as the concentration increased, and in the case of the ginsenoside Rb1 enriched fraction, the amount of collagen production increased and decreased again until the concentration increased to some extent. Therefore, it was confirmed that it is necessary to prepare a composition by setting an effective optimal concentration showing the efficacy of each substance and proceeded to optimization.

Table 2 below is based on the results of Table 1, the highest ratio of the Starfruit leaves extract, ginsenoside Rb1 enriched fraction, coastal bark extract in order to set the ratio is mixed in a specific ratio as in Example 7 Composition D was prepared to measure procollagen production. It can be confirmed that significantly higher procollagen was produced as compared with the results of experiments using such a single substance.

division Concentration (ug / mL) Procollagen Production (%)
Composition D (Example 7) 6.25 115 25 126 100 133

Example  9: Collagenase  Measurement of inhibitory activity

In order to confirm the trend of the experimental results in Example 8 in other indicators related to skin wrinkles, the inhibitory activity of collagenase involved in collagen degradation was measured.

MMP-1 (collagenase type IV from clostridium histolyticum, Sigma) and Pz-peptide (Pz-peptide; Bachem Feinckemikalien AG) were mixed with a sample dissolved in 0.1 mol / L Tris-HCl buffer containing 20 mmol / mL calcium chloride. The reaction was carried out for 30 minutes at. After the reaction, 20 mmol / L citric acid solution was added to stop the reaction, and ethyl hydrochloride was added and mixed. After centrifugation, the ethyl hydrochloride layer was measured at an absorbance of 320 nm, and a blank sample treated in the same manner was used as a control.

As shown in Table 3 below, the degree of collagenase inhibition was expressed as collagenase inhibition rate (%).

division Concentration (μg / mL) Collagenase inhibition rate (%)
Starfruit Leaf Extract 25 8.1 100 47.3 400 77.8
Coastal Pine Bark Extract 25 71.1 100 86.1 400 93.2
Ginsenoside Rb1 Enriched Fraction 25 - 100 - 400 2.0 Composition D
Example 7 25 21.7 100 67.1 400 83.0

As can be seen in Table 3, the collagenase inhibitory activity when treated with each single substance was found to be high in the order of the seaside pine bark extract, starfruit leaf extract, ginsenoside Rb1 enhanced fraction.

Based on the experimental results in Example 8, when treating the composition D prepared in the optimum ratio, it can be determined that the collagenase inhibitory activity showed a significantly higher efficacy compared to the concentration when treated with a single substance Can be. That is, the composition D containing only a small amount of coastal bark extract compared to the concentration of the coastal bark extract in a single material compared to the coastal bark extract showed the highest collagenase inhibitory activity when treated with a single material It exhibits synergistic effect with Starutz leaf extract and Ginsenoside Rb1 enhanced fraction in collagenase inhibitory activity.

Experimental Example  1: Animal Experiment

1) Experimental method

10-week-old HOS: HR-1 species of hairless mouse females per group, normal control group not irradiated with UV (AIN-76A diet, n = 10), positive control group irradiated with UV (AIN-76A diet, n = 10 ), UV and irradiated and divided into five groups of the intake group (each n = 10) ingesting the diet containing the starfruit leaf extract, seaweed pine bark extract and ginsenoside Rb1 enriched fraction, respectively.

After adapting to the groups for one week while providing a solid feed, the three substances were mixed with the AIN-76A diet and subjected to UV irradiation to measure the extent of skin wrinkles.

The UV irradiation was investigated in mice in four groups except for the main three times normal controls with the start of the test, starting with a dose of 54mJ / cm ² in the Week 1 was increased up to 90mJ / cm ^2, UV total dose examined 10 weeks are About 2646 mJ / cm ^{2 It} was made. During the 10 week test period, the extent of skin wrinkles caused by ultraviolet light was measured and identified as a biomarker.

2) Experiment result

In order to compare the degree of wrinkles caused by UV rays, skin wrinkle models (replicas) at the hips of mice not irradiated with UV rays and skin wrinkles of mice irradiated with UV rays were prepared. Ten weeks after the ingestion of the test substance, the skin wrinkles were measured by using a skin wrinkle measuring device (ASA-3RXD, ASAHI BIOMED) in each group of skin wrinkles, and the results are shown in Table 4. The extent of wrinkles was analyzed as wrinkle volume fraction (volume of wrinkles), maximum depth, and average depth.

group UV non-irradiated group UV irradiation group Normal control Positive control group Starfruit Leaf Extract Seaside Pine Bark
extract Ginsenoside Rb1 Enriched Fraction Wrinkle volume ratio 3.1 ± 0.75 7.7 ± 1.40 5.1 ± 1.55 5.0 ± 0.81 5.1 ± 0.80 Maximum Depth 156.6 ± 5.10 169.9 ± 5.78 161.1 ± 4.83 158.5 ± 9.06 151.7 ± 7.77 Wrinkle depth 57.8 ± 2.19 58.0 ± 1.45 58.6 ± 2.20 56.1 ± 2.04 54.0 ± 1.41

As a result, a significant (p <0.05) difference was observed in the wrinkle volume fraction index of the normal control group and the UV irradiated positive control group. Depth tended to decrease but no significance was confirmed. Among the three substance intake groups, the effect of the ginsenoside Rb1 enriched fraction was found to be the highest.

Experimental Example  2: animal experiment

1) Experimental method

A hairless mouse was used as an animal model to study the effect of the composition of the present invention on skin changes such as skin wrinkles, skin elasticity and skin moisture content generated by ultraviolet rays.

10 6-week-old females of HR-1 type hairless mice per group, normal control group without UV irradiation (AIN-76A diet, n = 10), UV control positive control group (AIN-76A diet, n = 10), UV A total of six groups of the ingested groups (compositions A, B, C, and D) of the investigated examples 4 to 7 (each n = 10) were divided.

After adapting to the groups for one week while providing solid feed, the composition A, B, C and D prepared according to Examples 4 and 7 were mixed with the AIN-76A diet and ingested with UV irradiation, respectively, to the skin. The change was measured.

The UV irradiation was performed to five groups of mice except the normal control three times a week with the start of the test. At week 1, the dose was gradually increased, with a dose of 48mJ / cm ² , and the total UV dose for mice was approximately 6000mJ / cm ² for a total of 12 weeks.

During the 12 week test period, skin wrinkle measurement, skin elasticity measurement and skin moisture content generated by ultraviolet light were measured and used as biomarkers.

2) Experiment result

A. skin Wrinkle  Measure

In order to compare the degree of wrinkles caused by UV rays, skin wrinkle models (replicas) at the hips of mice not irradiated with UV rays and skin wrinkles of mice irradiated with UV rays were prepared. After 10 weeks of ingestion of the test substance, skin wrinkles were measured by using a skin wrinkle measuring device (skin visiometer) in each group of skin wrinkles, and the results are shown in FIGS. 1 and 2. In FIG. 1, R1-R5, which is a result indicator, is an indicator indicating the maximum depth of wrinkles, the average depth, and the like.

As a result, a significant difference (p <0.05) was found in the wrinkle index of the normal control group and the UV irradiation positive control group, and a significant (p <0.05) difference was also found in the wrinkle index of the UV irradiation positive control group and the test substance intake group. Confirmed. In addition, the depth of the wrinkles of the composition D intake group was obtained significantly smaller than the UV irradiation positive control, the average wrinkles in the R2 ~ R5 obtained a result.

B. skin Elasticity  Measure

In order to measure the degree of elasticity of the skin changed by ultraviolet rays, the skin elasticity was measured by using a skin elasticity measuring device (CUTOMETER; Courage Khazaka, Germany) at the hip area of the mouse 8 weeks after ingestion of the test substance.

The measurement conditions of the cutometer were analyzed as the indicators of R0 to R9 by repeating the measurement of sound pressure 50 mbar, 1 second after 1 second of inhalation. Indicated.

UV irradiation showed significantly decreased elasticity factors R0, R3 and R8 compared to the normal group, and the results of the tendency to recover the skin elasticity level decreased by UV irradiation in the composition C intake group and the composition D intake group. Showed.

group

UV non-irradiated group UV irradiation group Normal control Positive control Composition A
Intake group Composition B
Intake group Composition C
Intake group Composition D
Intake group R0 0.187 ± 0.021 0.069 ± 0.007 0.132 ± 0.009 0.135 ± 0.014 0.155 ± 0.019 0.199 ± 0.024 R3 0.198 ± 0.024 0.071 ± 0.008 0.144 ± 0.009 0.147 ± 0.014 0.164 ± 0.029 0.182 ± 0.031 R8 0.153 ± 0.022 0.052 ± 0.006 0.095 ± 0.011 0.09 ± 0.015 0.121 ± 0.019 0.157 ± 0.022

C. Determination of Skin Moisture

In order to confirm the change of moisture content in the skin by ultraviolet rays, the moisture content in the skin was measured by using a corneometer (CORNEOMETER; Courage Khazaka, Germany) at the hip region of the mouse 7 weeks after the ingestion of the test substance.

As a result, the skin moisture content after 7 weeks was as Table 6 below. In the group irradiated with UV, the moisture content was significantly decreased compared to the group not irradiated with UV. The composition A intake group and the composition B intake group containing the starfruit leaf extract showed a tendency to protect the skin moisture content.

group UV non-irradiated group UV irradiation group Normal Positive control group Composition A Intake Group Composition B Intake Group Composition C Intake Group Composition D Intake Group Skin moisture content
[AU] 43.2 ± 2.33 34.9 ± 1.60 36.7 ± 2.10 35.8 ± 1.98 27.3 ± 1.80 33.2 ± 2.12

Claims (10)

A skin food composition for preventing skin wrinkles, comprising two or more of starfruit leaf extract, ginsenoside Rb1 enhanced fraction, or seaside pine peel extract. The method of claim 1,
Beauty food composition for preventing skin wrinkles, characterized in that it comprises a star fruit leaf extract and seaside pine peel extract. The method of claim 2,
A composition for preventing skin wrinkles, comprising 70 to 90% by weight of the starfruit leaf extract and 10 to 30% by weight of seaweed pine extract, based on the total weight of the composition. The method of claim 1,
A cosmetic composition for preventing skin wrinkles, comprising a star fruit leaf extract and a ginsenoside Rb1 enhanced fraction. The method of claim 4, wherein
A cosmetic composition for preventing skin wrinkles, comprising 65 to 85 wt% of the starfruit leaf extract and 15 to 35 wt% of the ginsenoside Rb1 enhanced fraction, based on the total weight of the composition. The method of claim 1,
A skin care composition for preventing skin wrinkles, comprising coastal bark extract and ginsenoside Rb1 enhanced fraction. The method of claim 6,
Skin cosmetic composition for preventing skin wrinkles, characterized in that containing 25 to 45% by weight of the coast pine bark extract and 55 to 75% by weight of the ginsenoside Rb1 enhanced fraction. The method of claim 1,
Starfruits leaf extract, ginsenoside Rb1 enhanced fraction and skin care composition for preventing skin wrinkles, characterized in that it comprises a coastal bark extract. The method of claim 8,
The total weight of the composition, 50 to 60% by weight of the starfruit leaf extract, 20 to 30% by weight of the ginsenoside Rb1 enhanced fraction and 10 to 20% by weight of the seaside pine peel extract for preventing skin wrinkles Food composition. A cosmetic food comprising the composition according to any one of claims 1 to 9.

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