KR20110060439A - Portable Fluorescence and Spectroscopic Analysis Device - Google Patents

Abstract

The present invention relates to a portable fluorescence and spectroscopic analysis device capable of simultaneous fluorescence analysis and spectroscopic analysis. A portable fluorescence and spectroscopic analysis device according to the present invention includes a main body case having an accommodation space therein; An LED light source module installed inside the main body case to emit measurement light; A spectroscopic sensor module which is accommodated in the main body case and senses the measurement light transmitted through a sample container containing a test sample which is emitted from the LED light source module and is a measurement object; A fluorescence sensor module accommodated in the body case to be disposed above the sample container and detecting fluorescence emitted by a test sample contained in the sample container; A light amount adjusting module disposed on an optical path of the measurement light between the LED light source module and the spectroscopic sensor module to adjust an amount of light of the measurement light directed to the sample container; A container driving module installed inside the main body case to support the sample container, and to move the sample container up and down so that the sample container is positioned on the optical path of the measurement light between the spectroscopic sensor module and the light quantity control module; Characterized in that it comprises a. Accordingly, while being portable, the fluorescence analysis and the spectroscopic analysis of the test sample can be simultaneously performed.

Description

PORTABLE FLUORESCENT AND SPECTROSCOPIC ANALYZING APPARATUS

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a portable fluorescence and spectroscopic analysis apparatus, which is portable and capable of simultaneously performing fluorescence and spectroscopic analysis of a test sample.

A spectroscopic apparatus is used for the long term and / or qualitative analysis of the test sample material, in particular in the solution. In general, the spectroscopic analyzer is a device for measuring the distribution of light according to the wavelength of light. Since the wavelength of light is absorbed, transmitted, and reflected by a test sample, the physical and chemical properties of all materials reacting with the light are examined. It is widely used to

A general spectroscopic analyzer includes a light source module that emits measurement light of a specific wavelength, and a spectroscopic sensor module that senses measurement light emitted from the light source module and transmitted through the test sample.

On the other hand, fluorescence analysis is used as another analysis method used for the measurement of a test sample. Fluorescence spectroscopy is used in the fields of biotechnology (DNA / Protein / Cell proliferation analysis, Histamine analysis, Algae Monitor), environmental sample analysis (wastewater, surface water tracer, hydrocarbon compound detection, dissolved oxygen measurement, etc.), dye and luminescent analysis It is no less versatile.

It is also used to study macromolecule properties and interactions with other materials. In particular, fluorescence is very sensitive and can be analyzed with a small amount of samples, which is useful for studying macromolecule conformation, binding site, solvent interaction, flexibility, and distance between molecules.

The principle of fluorescence analysis is that molecules that reach an excited state by absorption of light lose energy and return to a stable ground state. This energy conversion process is generally based on a non-radiative transition or intermolecular energy transfer which releases energy as heat by collision of molecules or the like. However, some molecules with a certain structure have a radiation process that emits energy back to light.

When these transitions occur between the same multiplicity, these molecules are called fluorescent materials and the light emitted is called fluorescence. From this spectral form of the emitted light, qualitative analysis of each substance can be further performed by quantitative analysis. Fluorescence spectroscopy has a limited scope of application compared to spectroscopy, but its sensitivity is one to three steps higher than spectrophotometry.

As described above, the spectroscopic analysis and the fluorescence analysis are different from each other, and when the fluorescence analysis and the spectral analysis are performed on the same test sample, the fluorescence analysis is performed on the test sample, and the spectral analysis device is tested. Spectroscopic analysis is performed on the sample.

However, if the fluorescence spectrometer and the spectroscopic analyzer are not included together, or if the fluorescence spectrometer and the spectroscopic analyzer are installed in different places, the test sample may be placed in two sample containers for separate fluorescence and spectroscopic analysis. Will proceed. This causes two measurements in different places to analyze a test sample, resulting in inconvenience in terms of time and staffing.

Accordingly, the present invention has been made to solve the above problems, while being able to carry out fluorescence analysis and spectroscopic analysis on one test sample at the same time, it is portable so that fluorescence analysis and spectroscopy can be performed regardless of place. It is an object of the present invention to provide a portable fluorescence and spectroscopic analysis device capable of performing the analysis simultaneously.

In addition, the present invention provides a portable fluorescence and spectroscopic analysis device capable of ensuring the accuracy of spectroscopic and fluorescence analysis while reducing the overall size of the portable fluorescence and spectroscopic analysis device by enabling the use of a small sample container. There is a purpose.

According to the present invention, a portable fluorescence and spectroscopic analysis device capable of simultaneous fluorescence analysis and spectroscopic analysis, comprising: a body case having an accommodation space therein; An LED light source module installed inside the main body case to emit measurement light; A spectroscopic sensor module which is accommodated in the main body case and senses the measurement light transmitted through a sample container containing a test sample which is emitted from the LED light source module and is a measurement object; A fluorescence sensor module accommodated in the body case to be disposed above the sample container and detecting fluorescence emitted by a test sample contained in the sample container; A light amount adjusting module disposed on an optical path of the measurement light between the LED light source module and the spectroscopic sensor module to adjust an amount of light of the measurement light directed to the sample container; A container driving module installed inside the main body case to support the sample container, and to move the sample container up and down so that the sample container is positioned on the optical path of the measurement light between the spectroscopic sensor module and the light quantity control module; It is achieved by a portable fluorescence and spectroscopic analysis device comprising a.

Here, the container drive module, the container insertion portion is inserted into one side of the sample container; A first container driving unit which vertically moves the container inserting portion to vertically move the sample container; A second vessel driver for reciprocating the vessel insert to reciprocate the sample vessel in an optical path direction; The sample container may include a third container driver for reciprocating the container insert so that the sample container reciprocates in a direction perpendicular to the optical path direction and the vertical direction.

The apparatus may further include a fluorescent support frame installed on the container driving module to support the fluorescent sensor module so that the fluorescent sensor module is disposed to be spaced apart from the upper portion of the sample container.

In addition, the first vessel driver, the second vessel driver and the third vessel driver may be provided in the form of a PZT actuator.

In addition, a container passage opening through which the sample container passes through the sample container may be inserted into the container insert from the outside of the main body case to be inserted into the container inserting part.

The LED light source module includes a plate-shaped rotating body; A plurality of LEDs installed in the rotation main body in a state spaced apart from each other along a circumferential direction and emitting measurement light having mutually different wavelengths; One side is connected to the rotation main body and the other side is rotatably installed on the main body case so as to be exposed to the outside of the main body case is a rotation to rotate the main body so that any one of the plurality of LED is located in front of the light quantity control module. It may include a lever.

The light amount adjusting module may include: a first aperture plate and a second aperture plate that cross each other to form a light passing hole through which the measurement light passes; An aperture bracket installed inside the main body case to support at least one of the first aperture plate and the second aperture plate to slidably reciprocate in a direction crossing each other; It may include a light amount adjusting pin for adjusting the amount of light of the measurement light by sliding reciprocating at least one of the first aperture plate and the second aperture plate along the aperture bracket so that the size of the light passing hole is adjusted. .

The light quantity adjusting module further includes a pin bracket rotatably supporting the light quantity adjusting pin; The light amount adjusting pin may be formed with an interlocking thread at a position corresponding to an outer diameter and the first diaphragm plate so that the first diaphragm plate may slide along the diaphragm bracket according to the rotation of the light amount adjusting pin.

According to the present invention, it is possible to carry out fluorescence analysis and spectroscopic analysis on one test sample at the same time, while being portable so that portable fluorescence and spectroscopic analysis can be performed at the same time regardless of location. An apparatus is provided.

In addition, according to the present invention, it is possible to use a sample container of a small size to reduce the overall size of the portable fluorescence and spectroscopic analysis device, while ensuring the accuracy of the spectroscopic and fluorescence analysis.

Hereinafter, with reference to the accompanying drawings will be described in detail an embodiment according to the present invention. 1 is a perspective view of a portable fluorescence and spectroscopic analysis apparatus 100 according to the present invention, Figure 2 is an exploded perspective view of the portable fluorescence and spectroscopic analysis apparatus 100 of FIG.

1 and 2, the portable fluorescence and spectroscopic analysis device 100 according to the present invention includes a main body case 10, an LED light source module 20, a spectroscopic sensor module 51, and a fluorescent sensor module ( 60 a, see FIG. 7), the light amount control module 30, and the container drive module 40.

Body case 10 forms the overall appearance of the portable fluorescence and spectroscopic analysis device 100 according to the present invention, the LED light source module 20, the spectroscopic sensor module 51, the light amount control module 30 and the container drive module An accommodation space for accommodating therein the components constituting the portable fluorescence and spectroscopic analysis apparatus 100 according to the present invention, such as 40, is formed.

Here, the main body case 10 according to the present invention includes a lower case 12 forming a bottom surface, and an upper case 11 connected to the lower case 12 from the top to form an accommodation space therein. For example.

As shown in FIG. 2, the LED light source module 20 is installed inside the main body case 10. Here, the LED light source module 20 according to the present invention is provided to selectively emit the measurement light of a plurality of wavelength ranges. As shown in FIG. 3, the LED light source module 20 according to the present invention may include a pivoting body 21, a plurality of LEDs (not shown), and a pivoting lever 23.

The rotating body 21 has a plate shape. In FIG. 3, the rotation main body 21 has the disk shape as an example. Here, the rotation main body 21 is formed with a plurality of LED receiving holes 22 formed spaced apart from each other in the circumferential direction.

The plurality of LEDs emit measurement light of mutually different wavelengths. Each LED is accommodated in each LED accommodating hole 22 formed in the pivot body 21 and disposed to be spaced apart from each other along a circumferential direction of the pivot body 21.

Here, in the present invention, for example, each LED is provided to irradiate the measurement light of the wavelength of any one of 340nm, 370nm, 405nm, 460nm, 520nm, 605nm and 850nm. Accordingly, when any one of the plurality of LEDs is disposed in front of the light amount control module 30 side when the rotation main body 21 rotates, the measurement light of the corresponding wavelength can be emitted to the sample container 110 side in which the test sample is accommodated. Done.

Accordingly, by rotating the rotating body 21 to selectively emit the measurement light of a specific wavelength, by using the portable fluorescence and spectroscopic analysis apparatus 100 according to the present invention, spectroscopic analysis and fluorescence analysis of various types of test fountains This becomes possible. Here, the respective wavelength ranges are merely examples, and of course, it is possible to apply LEDs having different wavelength values.

On the other hand, one side of the rotation lever 23 is connected to the rotation main body 21. The other side of the rotation lever 23 is exposed to the outside of the main body case 10 as shown in FIG. 2. In addition, the rotation lever 23 is rotatably provided in the main body case 10.

Here, the lever passage hole 13 is formed in the main body case 10 so that the other side of the rotation lever 23 can be exposed from the inside of the main body case 10 to the outside. And the guide groove 24 is formed in the outer surface of the rotation lever 23 along the circumferential direction, and the rotation lever 23 is a main body by inserting the inner diameter edge of the lever passage hole 13 in the guide groove 24. It is installed in the case 10 so that rotation is possible.

Through the configuration as described above, when the user grips and rotates the other edge of the rotation lever 23 exposed to the outside of the main body case 10, the rotation main body 21 connected to the rotation lever 23 rotates, Any one of the plurality of LEDs arranged along the circumferential direction on the plate surface of the rotating body 21 is located in front of the light amount control module 30 to emit the measurement light of the corresponding wavelength band toward the light amount control module 30. do.

On the other hand, the light amount adjusting module 30 is disposed between the light path of the measurement light between the LED light source module 20 and the spectroscopic sensor module 51. The light amount adjusting module 30 adjusts the light amount of the measurement light emitted from the LED light source module 20 toward the sample container 110.

The light amount adjusting module 30 according to the present invention, as shown in FIG. 4, includes a first aperture plate 31, a second aperture plate 32, an aperture bracket 33, and an amount of light adjustment pin 35. can do.

The first aperture plate 31 and the second aperture plate 32 cross each other to form a light passing hole 37 through which measurement light passes. More specifically, referring to FIG. 5, the first diaphragm 31 is formed with a first half-shaped first recessed portion 31a toward the second diaphragm 32, and the second diaphragm 32 is formed. The second recessed portion 32a having a half rhombus shape is formed toward the first diaphragm 31. Accordingly, when the first aperture plate 31 and the second aperture plate 32 move in a direction that is spaced apart from each other, the size of the light passage hole 37 increases, whereas when the first aperture plate 31 and the second aperture plate 32 move in a direction that crosses each other, the light passage hole 37 ) Becomes small, and the amount of light of the measurement light passing through the light passage hole 37 can be adjusted.

Here, the aperture bracket 33 is installed inside the main body case 10 to support at least one of the first aperture plate 31 and the second aperture plate 32 so as to slidably reciprocate in a direction crossing each other. . In FIG. 4, the cross-sectional shape of the aperture bracket 33 has an approximately 'C' shape, and the first aperture plate 31 and the second aperture plate 32 are inserted to be slidably reciprocated. In addition, the aperture bracket 33 is formed with a light traveling hole 33a so that the measurement light from the LED light source module 20 can face the light passing hole 37.

On the other hand, the light amount adjusting pin 35 reciprocates sliding at least one of the first aperture plate 31 and the second aperture plate 32 along the aperture bracket 33 so that the size of the light passage hole 37 is adjusted. By adjusting the amount of light of the measurement light. Here, in the present invention, the first aperture plate 31 and the second aperture plate 32 are all slidably reciprocated by the light amount adjusting pin 35, for example, the first aperture plate 31 or the second Of course, only the aperture plate 32 may be provided to be slidably reciprocating.

More specifically, the light amount adjusting module 30 may include pin brackets 34a and 34b rotatably supporting the light amount adjusting pin 35. Then, at the positions corresponding to the outer diameter of the light amount adjusting pin 35 and the first diaphragm plate 31, a threaded portion 36a is formed. Accordingly, when the light amount control pin 35 is rotated, the first aperture plate 31 is reciprocated by the interlocking threads 36a to adjust the size of the light passage hole 37. Here, a screw thread (not shown) formed in the first aperture plate 31 and the second aperture plate 32 is provided in the through hole through which the light amount adjusting pin 35 passes, as shown in FIG. 4. Take this as an example.

In addition, as shown in FIG. 1, the edge region of the light amount adjusting pin 35 is not exposed to the outside of the main body case 10, but the external area of the light amount adjusting pin 35 may be operated. It is a matter of course that the edge region of the light quantity modulating pin 35 is exposed to the outside of the main body case 10.

As described above, by adjusting the light amount of the measurement light directed to the sample container 110 through the light amount control module 30, to reduce the size of the image formed on the sample container 110 side, past the test sample received in the sample container 110 It is possible to improve the accuracy of the spectroscopic analysis by blocking the measurement light directed to the spectroscopic sensor module 51 without.

Here, as shown in FIG. 4, the light amount adjusting pin 35 is installed through the pin brackets 34a and 34b, the first aperture plate and the second aperture plate 32 in sequence, thereby providing a more stable operation. It may be provided to enable.

On the other hand, the spectroscopic sensor module 51 is accommodated in the main body case 10, and detects the measurement light emitted from the LED light source module 20 and passed through the sample container 110 through the light amount control module 30. . The spectroscopic sensor module 51 may be provided in the form of a silicon photodiode, and information about the measurement light detected by the spectroscopic sensor module 51 is transmitted to the fluorescence and spectroscopic analysis processing module 92 which will be described later.

Here, the spectral sensor module 51 is supported by the spectral support frame 52, as shown in FIG. The spectroscopic support frame 52 is installed upward from the bottom surface of the main body case 10 to support the spectroscopic sensor module 51 so that the spectroscopic sensor module 51 is positioned on the optical path of the measurement light.

Meanwhile, the fluorescence sensor module 60a is disposed above the sample container 110 to detect fluorescence emitted by the test sample accommodated in the sample container 110. Here, the fluorescent sensor module 60a is supported by the fluorescent support frame 60 and disposed to be spaced apart from the upper portion of the sample container 110. In the present invention, the fluorescent support frame 60 is the container drive module 40. Is connected to the upper portion of the support shape sensor module is an example.

Accordingly, even when the vessel driving module 40 reciprocates in the ZYX direction, which will be described later, the fluorescence support frame 60 reciprocates together, so that the separation distance between the fluorescence sensor module 60a and the sample vessel 110 or the XY plane may be reduced. The position will not change.

According to the above configuration, the portable fluorescence and spectroscopic analysis apparatus 100 according to the present invention can be performed selectively or simultaneously with spectral analysis and fluorescence analysis. Here, the information on the fluorescence sensed by the fluorescence sensor module 60a is transmitted to the fluorescence and spectroscopic analysis processing module 92 which will be described later.

For example, in connection with the development of DNA diagnostic methods for pathogens using polymerase chain reaction (PCR), polymerase chain reaction (PCR) is a method for the in vitro synthesis of specific DNA sites. It is a method of amplifying a desired DNA molecule in a large amount of DNA synthesis using a very small amount of DNA.

Polymerase Chain Reaction (PCR) can amplify DNA in a specific region up to hundreds of millions of times in hours in vitro, and this amplified DNA can be used for various kinds of experiments. It can be applied to various medical studies.

As a method for analyzing DNA, a portable fluorescence and spectroscopic analysis device according to the present invention can be used. For application of the fluorescence and spectroscopic analysis method, the measurement light of 490 nm wavelength is irradiated from the LED light source module 20 to the sample container 110 in which the sample DNA and the reagent GREEN-I are mixed.

When the measurement light irradiated from the LED light source module 20 is incident to the sample container, the fluorescent sensor module 60a of the DNS and GREEN-I, which is a test sample accommodated in the sample container 110, is angled at a 90 ° angle with the traveling direction of the measurement light. Fluorescence emitted by the mixed solution is detected to fluoresce the concentration of DNA. At the same time, the spectroscopic sensor module 51 causes the concentration of GREEM-I, which is a reagent, to be spectroscopically analyzed at an angle of 180 degrees to the traveling direction of the measurement light.

FIG. 6 illustrates an example in which 510 nm wavelength fluorescence is sensed by the fluorescent sensor module 60a by the fluorescent material of the test sample when the measurement light of 490 nm wavelength is emitted from the LED light source module 20.

As described above, by simultaneously measuring the fluorescence analysis and the spectroscopic analysis, it is possible to verify whether the dispensing of the reagent GREEN-I is correct or completely mixed with respect to the measurement of the concentration of the sample DNA. Thus, precision and accuracy can be provided for DNA and reagent (GREEN-I) dispensing.

Hereinafter, the sample container 110 and the container driving module 40 according to the present invention will be described with reference to FIG. 7.

Sample container 110 according to the present invention is an example having a rectangular parallelepiped shape that can accommodate a test sample therein. And, the sample container 110 has a shape whose thickness is relatively thinner than the width.

The vessel driving module 40 is installed inside the body case 10 to support the sample vessel 110 so that the sample vessel 110 is positioned on the optical path of the measurement light. At this time, the sample container 110 is supported by the container drive module 40 so that the thickness direction is located on the optical path of the measurement light and the width direction is located in the up-down direction, as shown in FIG.

In addition, the container driving module 40 moves the sample container 110 up and down on the optical path of the measurement light between the spectroscopic sensor module 51 and the light amount control module 30. That is, the container driving module 40 adjusts the vertical position of the sample container 110, so that the side surface of the sample container 110 having a thickness that is relatively thinner than the width can be accurately positioned on the optical path of the measurement light. .

Referring to FIG. 7, the container driving module 40 according to the present invention includes a container inserting part 41 into which one side of the sample container 110 is inserted, a first container driving part 42 and a second container. The vessel driver 43 and the third vessel driver 44 may be included.

The container inserting portion 41 is provided in a form corresponding to the cross-sectional shape of the sample container 110. Here, the container passage opening 14 is formed on the side surface of the main body case 10 at a position corresponding to the container inserting portion 41 so that the sample container 110 can pass from the outside of the main body case 10 to the inside. Accordingly, the user inserts the sample container 110 from the outside of the main body case 10 through the container passage opening 14 formed in the main body case 10, and continues inserting the sample container 110 in the insertion direction. One side edge of the sample container 110 is inserted into the container insert 41 to be supported by the container driving module 40.

The first container drive part 42 moves the container insertion part 41 in the vertical direction so that the sample container 110 moves up and down. The second vessel driver 43 reciprocates the container insert 41 so that the sample vessel 110 reciprocates in the optical path direction, and the third vessel driver 44 moves the sample vessel 110 to the optical path. The container insertion part 41 is reciprocated so that it may reciprocate in the direction perpendicular | vertical to a direction and an up-down direction.

That is, the container inserting portion 41 can be reciprocated in the XYZ direction as the first container driving portion 42, the second container driving portion 43, and the third container driving portion 44 are driven, and the container inserting portion 41 The sample container 110 is capable of reciprocating in the XYZ direction on the optical path of the measurement light as the () moves.

Here, the first vessel driving unit 42, the second vessel driving unit 43 and the third vessel driving unit 44 according to the present invention is provided in the form of a PZT actuator, it is an example that the fine movement is provided. Accordingly, the position of the sample container 110 in the optical path of the measurement light can be finely adjusted to enable more accurate spectroscopic analysis.

On the other hand, inside the main body case 10, the above-described fluorescence and spectroscopic analysis processing module 92 for processing information on the measurement light detected by the spectroscopic sensor module 51, and a vessel driving module in the form of a PZT actuator ( PZT control module 91 for the control of 40 is housed. Here, the fluorescence and spectroscopic analysis processing module 92 and the PZT control module 91 are provided as one circuit board and accommodated as an example.

In addition, an upper portion of the main body case 10 may be provided with an LCD display unit 93 exposed to the outside. Accordingly, various information processed by the fluorescence and spectroscopic analysis processing module 92 is displayed on the display 93, so that the user can visually check necessary information.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

1 is a perspective view of a portable fluorescence and spectroscopic analysis device according to the present invention,

FIG. 2 is an exploded perspective view of the portable fluorescence and spectroscopic analysis device of FIG. 1;

3 is a view for explaining the LED light source module of the portable fluorescence and spectroscopic analysis device according to the present invention,

4 and 5 are views for explaining the light amount control module and the spectroscopic sensor module of the portable fluorescence and spectroscopic analysis apparatus according to the present invention,

6 is a view showing an example of fluorescence analysis measured by a portable fluorescence and spectroscopic analysis device according to the present invention,

7 is a view for explaining the container drive module of the portable fluorescence and spectroscopic analysis device according to the present invention.

<Description of Major Numbers in Drawing>

100: Portable Fluorescence and Spectroscopic Analysis Device

10: body case

11: upper case 12: lower case

13: lever passage hole 14: container passage hole

20: LED light source module 21: rotating body

22: LED receiving hole 23: rotation lever

24: guide groove 30: light intensity control module

31: first aperture plate 32: second aperture plate

33: aperture bracket 34a, 34b: pin bracket

35: light amount adjustment pin 37: light passing hole

40: container drive module 41: container insert

42: first vessel driver 43: second vessel driver

44: third vessel drive unit 51: spectroscopic sensor module

52 spectral support frame 60 fluorescence support frame

60a: fluorescent sensor module 91: PZT control module

92: Fluorescence and Spectroscopic Analysis Processing Module

93: display unit

Claims (8)

In the portable fluorescence and spectroscopic analysis device capable of simultaneous fluorescence analysis and spectroscopic analysis, A main body case having an accommodation space formed therein; An LED light source module installed inside the main body case to emit measurement light; A spectroscopic sensor module which is accommodated in the main body case and senses the measurement light transmitted through a sample container containing a test sample which is emitted from the LED light source module and is a measurement object; A fluorescence sensor module accommodated in the body case to be disposed above the sample container and detecting fluorescence emitted by a test sample contained in the sample container; A light amount adjusting module disposed on an optical path of the measurement light between the LED light source module and the spectroscopic sensor module to adjust an amount of light of the measurement light directed to the sample container; A container driving module installed inside the main body case to support the sample container, and to move the sample container up and down so that the sample container is positioned on the optical path of the measurement light between the spectroscopic sensor module and the light quantity control module; Portable fluorescence and spectroscopic analysis device comprising a. The method of claim 1, The container drive module, A container insertion unit into which one side of the sample container is inserted; A first container driving unit which vertically moves the container inserting portion to vertically move the sample container; A second vessel driver for reciprocating the vessel insert to reciprocate the sample vessel in an optical path direction; And a third vessel driver for reciprocating the vessel insert to reciprocate the sample vessel in a direction perpendicular to the optical path direction and the vertical direction. The method of claim 2, Portable fluorescence and spectroscopic analysis device is installed on top of the vessel driving module further comprises a fluorescence support frame for supporting the fluorescence sensor module so that the fluorescence sensor module is disposed spaced apart on the upper portion of the sample vessel. . The method of claim 2, And the first vessel driver, the second vessel driver, and the third vessel driver are provided in the form of a PZT actuator. The method of claim 2, Portable fluorescence and spectroscopy apparatus characterized in that the side of the main body case is formed with a container passage through which the sample container passes through the sample container is inserted from the outside of the main body case to be inserted into the container insert portion . 6. The method according to any one of claims 1 to 5, The LED light source module, A plate-shaped rotating body; A plurality of LEDs installed in the rotation main body in a state spaced apart from each other along a circumferential direction and emitting measurement light having mutually different wavelengths; One side is connected to the rotation main body and the other side is rotatably installed on the main body case so as to be exposed to the outside of the main body case is a rotation to rotate the main body so that any one of the plurality of LED is located in front of the light quantity control module. A portable fluorescence and spectroscopic analysis device comprising a lever. 6. The method according to any one of claims 1 to 5, The light amount control module, A first aperture plate and a second aperture plate which cross each other to form a light passing hole through which the measurement light passes; An aperture bracket installed inside the main body case to support at least one of the first aperture plate and the second aperture plate to slidably reciprocate in a direction crossing each other; And a light amount adjusting pin configured to adjust the amount of light of the measurement light by sliding reciprocally moving at least one of the first and second aperture plates along the aperture bracket so that the size of the light passing hole is adjusted. Portable fluorescence and spectroscopic analysis device. The method of claim 7, wherein The light quantity adjusting module further includes a pin bracket rotatably supporting the light quantity adjusting pin; The light quantity adjusting pin has a thread formed in engagement with each other at a position corresponding to an outer diameter and the first aperture plate so that the first aperture plate slides along the aperture bracket according to the rotation of the light quantity adjustment pin. And spectroscopic analysis device.

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