KR20100096447A - Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom - Google Patents
Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom Download PDFInfo
- Publication number
- KR20100096447A KR20100096447A KR1020090015330A KR20090015330A KR20100096447A KR 20100096447 A KR20100096447 A KR 20100096447A KR 1020090015330 A KR1020090015330 A KR 1020090015330A KR 20090015330 A KR20090015330 A KR 20090015330A KR 20100096447 A KR20100096447 A KR 20100096447A
- Authority
- KR
- South Korea
- Prior art keywords
- stem cells
- adult stem
- cosmetic composition
- pig
- tissue
- Prior art date
Links
- 239000002537 cosmetic Substances 0.000 title claims abstract description 52
- 210000004504 adult stem cell Anatomy 0.000 title claims abstract description 34
- 239000000203 mixture Substances 0.000 title claims abstract description 33
- 210000005059 placental tissue Anatomy 0.000 title claims abstract description 30
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 27
- 241000282898 Sus scrofa Species 0.000 title abstract description 29
- 238000004113 cell culture Methods 0.000 claims abstract description 18
- 150000001413 amino acids Chemical class 0.000 claims abstract description 15
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 9
- 239000004480 active ingredient Substances 0.000 claims abstract description 8
- 230000037303 wrinkles Effects 0.000 claims abstract description 8
- 230000006872 improvement Effects 0.000 claims abstract description 6
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 4
- 239000006071 cream Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 18
- 239000002609 medium Substances 0.000 claims description 14
- 239000006210 lotion Substances 0.000 claims description 11
- 239000000499 gel Substances 0.000 claims description 10
- 210000001691 amnion Anatomy 0.000 claims description 9
- 210000001519 tissue Anatomy 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 6
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 4
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 4
- 239000000686 essence Substances 0.000 claims description 4
- 230000003169 placental effect Effects 0.000 claims description 4
- 239000006143 cell culture medium Substances 0.000 claims description 2
- 210000000130 stem cell Anatomy 0.000 abstract description 13
- 239000000306 component Substances 0.000 description 25
- 235000018102 proteins Nutrition 0.000 description 21
- 210000004991 placental stem cell Anatomy 0.000 description 19
- 210000003491 skin Anatomy 0.000 description 19
- 210000002826 placenta Anatomy 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- 239000000839 emulsion Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 239000003921 oil Substances 0.000 description 10
- 235000019198 oils Nutrition 0.000 description 10
- -1 polyoxyethylene Polymers 0.000 description 9
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 8
- 230000003020 moisturizing effect Effects 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 235000014113 dietary fatty acids Nutrition 0.000 description 7
- 239000000194 fatty acid Substances 0.000 description 7
- 229930195729 fatty acid Natural products 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 6
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 6
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 6
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 6
- 235000011187 glycerol Nutrition 0.000 description 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 5
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 5
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 5
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000004909 Moisturizer Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 239000003102 growth factor Substances 0.000 description 4
- 229960000890 hydrocortisone Drugs 0.000 description 4
- 230000001333 moisturizer Effects 0.000 description 4
- 210000000434 stratum corneum Anatomy 0.000 description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000016359 Fibronectins Human genes 0.000 description 3
- 108010067306 Fibronectins Proteins 0.000 description 3
- 102000004877 Insulin Human genes 0.000 description 3
- 108090001061 Insulin Proteins 0.000 description 3
- 108010050808 Procollagen Proteins 0.000 description 3
- 235000010323 ascorbic acid Nutrition 0.000 description 3
- 229960005070 ascorbic acid Drugs 0.000 description 3
- 239000011668 ascorbic acid Substances 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 230000024245 cell differentiation Effects 0.000 description 3
- 230000032823 cell division Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- GPLRAVKSCUXZTP-UHFFFAOYSA-N diglycerol Chemical class OCC(O)COCC(O)CO GPLRAVKSCUXZTP-UHFFFAOYSA-N 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000003974 emollient agent Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 229920002674 hyaluronan Polymers 0.000 description 3
- 229960003160 hyaluronic acid Drugs 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 229940125396 insulin Drugs 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- XDOFQFKRPWOURC-UHFFFAOYSA-N 16-methylheptadecanoic acid Chemical compound CC(C)CCCCCCCCCCCCCCC(O)=O XDOFQFKRPWOURC-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010066486 EGF Family of Proteins Proteins 0.000 description 2
- 102000018386 EGF Family of Proteins Human genes 0.000 description 2
- OHCQJHSOBUTRHG-KGGHGJDLSA-N FORSKOLIN Chemical compound O=C([C@@]12O)C[C@](C)(C=C)O[C@]1(C)[C@@H](OC(=O)C)[C@@H](O)[C@@H]1[C@]2(C)[C@@H](O)CCC1(C)C OHCQJHSOBUTRHG-KGGHGJDLSA-N 0.000 description 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 2
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 2
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 102000011782 Keratins Human genes 0.000 description 2
- 108010076876 Keratins Proteins 0.000 description 2
- 102100020880 Kit ligand Human genes 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 102000036693 Thrombopoietin Human genes 0.000 description 2
- 108010041111 Thrombopoietin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000008168 almond oil Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000002316 cosmetic surgery Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 229940126864 fibroblast growth factor Drugs 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 229940119170 jojoba wax Drugs 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 210000002894 multi-fate stem cell Anatomy 0.000 description 2
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 238000007665 sagging Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000002374 sebum Anatomy 0.000 description 2
- 230000037394 skin elasticity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229920003169 water-soluble polymer Polymers 0.000 description 2
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 1
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 1
- FKOKUHFZNIUSLW-UHFFFAOYSA-N 2-Hydroxypropyl stearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(C)O FKOKUHFZNIUSLW-UHFFFAOYSA-N 0.000 description 1
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 235000019489 Almond oil Nutrition 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 description 1
- 102100031614 Ciliary neurotrophic factor Human genes 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- SUZLHDUTVMZSEV-UHFFFAOYSA-N Deoxycoleonol Natural products C12C(=O)CC(C)(C=C)OC2(C)C(OC(=O)C)C(O)C2C1(C)C(O)CCC2(C)C SUZLHDUTVMZSEV-UHFFFAOYSA-N 0.000 description 1
- 101001004391 Drosophila melanogaster Protein jim lovell Proteins 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000005792 Geraniol Substances 0.000 description 1
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- 108010023302 HDL Cholesterol Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 102100039064 Interleukin-3 Human genes 0.000 description 1
- 108010002386 Interleukin-3 Proteins 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 1
- 102000004140 Oncostatin M Human genes 0.000 description 1
- 108090000630 Oncostatin M Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 102000015731 Peptide Hormones Human genes 0.000 description 1
- 108010038988 Peptide Hormones Proteins 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 description 1
- 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 108010039445 Stem Cell Factor Proteins 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000012914 anti-clumping agent Substances 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 229940121363 anti-inflammatory agent Drugs 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000003212 astringent agent Substances 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 229940092738 beeswax Drugs 0.000 description 1
- 239000012166 beeswax Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000010495 camellia oil Substances 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- OHCQJHSOBUTRHG-UHFFFAOYSA-N colforsin Natural products OC12C(=O)CC(C)(C=C)OC1(C)C(OC(=O)C)C(O)C1C2(C)C(O)CCC1(C)C OHCQJHSOBUTRHG-UHFFFAOYSA-N 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 229940105990 diglycerin Drugs 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000010696 ester oil Substances 0.000 description 1
- 229940093476 ethylene glycol Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 108700014844 flt3 ligand Proteins 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 229940113087 geraniol Drugs 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 208000024963 hair loss Diseases 0.000 description 1
- 230000003676 hair loss Effects 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 1
- 230000024278 histolysis Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002608 insulinlike Effects 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 210000004993 mammalian placenta Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 1
- 229960003987 melatonin Drugs 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 1
- 239000002077 nanosphere Substances 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 229940055577 oleyl alcohol Drugs 0.000 description 1
- XMLQWXUVTXCDDL-UHFFFAOYSA-N oleyl alcohol Natural products CCCCCCC=CCCCCCCCCCCO XMLQWXUVTXCDDL-UHFFFAOYSA-N 0.000 description 1
- 239000007968 orange flavor Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- WXZMFSXDPGVJKK-UHFFFAOYSA-N pentaerythritol Chemical compound OCC(CO)(CO)CO WXZMFSXDPGVJKK-UHFFFAOYSA-N 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 229940021222 peritoneal dialysis isotonic solution Drugs 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 229920000223 polyglycerol Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000012254 powdered material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229960004063 propylene glycol Drugs 0.000 description 1
- 229940093625 propylene glycol monostearate Drugs 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 150000004728 pyruvic acid derivatives Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 229940031439 squalene Drugs 0.000 description 1
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229940037128 systemic glucocorticoids Drugs 0.000 description 1
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
- 230000008354 tissue degradation Effects 0.000 description 1
- 210000003014 totipotent stem cell Anatomy 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
- 230000007998 vessel formation Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000004520 water soluble gel Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
Abstract
본 발명은 유효성분으로 돼지 태반 조직 유래 성체 줄기세포 배양물; 또는 상기 돼지 태반 조직 유래 성체 줄기세포로부터 추출된 단백질, 또는 상기 단백질의 가수분해물인 펩타이드나 아미노산을 추가로 함유하는 화장료용 조성물에 관한 것이다. 본 발명에 따른 화장료용 조성물은 돼지 태반 조직 유래 성체 줄기세포 배양물과 상기 줄기세포로부터 추출한 단백질 또는 아미노산이 주름 개선 및 노화 방지에 있어서 상승작용적으로 상호작용하므로, 기능성 화장품 제조에 유용하다.The present invention is an adult stem cell culture derived from pig placental tissue as an active ingredient; Or it relates to a cosmetic composition further comprising a protein extracted from the pig stem tissue derived adult stem cells, or a peptide or amino acid that is a hydrolyzate of the protein. The cosmetic composition according to the present invention is useful for producing functional cosmetics, since the adult stem cell culture derived from pig placental tissue and the protein or amino acid extracted from the stem cells synergistically interact in wrinkle improvement and anti-aging.
Description
본 발명은 (i) 돼지 태반 조직 유래 줄기세포 배양물 및 (ii) 상기 돼지 태반 조직 유래 성체 줄기세포로부터 추출된 단백질; 또는 상기 단백질의 가수분해물인 펩타이드나 아미노산을 함유하는 화장료용 조성물 및 그 제조방법에 관한 것이다.The present invention provides a protein extracted from (i) porcine placental tissue-derived stem cell culture and (ii) adult porcine placental tissue-derived adult stem cells; Or it relates to a cosmetic composition containing a peptide or amino acid which is a hydrolyzate of the protein and a method for producing the same.
줄기세포(stem cell)란 자기 복제 능력을 가지면서 두 개 이상의 세포로 분화하는 능력을 갖는 세포로, 만능 줄기세포(totipotent stem cell), 전분화능 줄기세포(pluripotent stem cells), 다분화능 줄기세포(multipotent stem cells)로 분류할 수 있다. Stem cells are cells that have the ability to self-replicate and differentiate into two or more cells. Totipotent stem cells, pluripotent stem cells, and multipotent stem cells ( multipotent stem cells).
태반(placenta)은 임신한 포유류의 자궁에 생기는 기관으로 탯줄을 통하여 태아에게 영양분을 공급하고, 생명을 지탱하게 한다. 태반은 출산시 자궁으로부터 배출되며, 태반이 나오는 것을 후출산이라고 한다. 태반에는 필수 아미노산, 멜라토닌, RNA, DNA 등의 핵산 성분, 항산화 효소인 SOD(Super oxide Dismwtase), 히알루론산(Hyaluronic acid), 항산화제, 면역보조인자(cytocine), 태반펩타이드, 인슐린유사성장촉진인자(insulin-like-grouth factor), 표피성장촉진인자(EGFS, Epidermal Grouth factors) 및 독특한 노쇠세포활성인자(SCAFS, Senescent cellAdivating factors) 등의 성장인자와 사이토카인 류가 포함되어 있어 피로회복, 면역증강 등에 유용한 것으로 알려져 있다.The placenta is an organ that develops in the womb of a pregnant mammal and nourish and sustain life through the umbilical cord. The placenta is discharged from the uterus at birth and the placenta is called postpartum. The placenta contains nucleic acids such as essential amino acids, melatonin, RNA and DNA, superoxide dismwtase (SOD), hyaluronic acid (antioxidants), antioxidants, cytokines, placental peptides and insulin-like growth promoters. (Insulin-like-grouth factor), epidermal growth factors (EGFS) and unique senescent cell activating factors (SCAFS), including cytokines and growth factors It is known to be useful.
또한, 태반의 조직 대사 촉진작용이 피지의 분비선 기능을 높여 피지의 분비를 정상화한 결과 잔주름을 줄이거나 제거하는 작용을 한다. 아울러, 태반에는 섬유아 세포의 증식인자(FGF)가 포함되어 있어 섬유아 세포의 증식, 재생을 촉진하며 이의 활성화를 통해 콜라겐, 엘라스틴, 히아루론산의 양을 증가시켜 피부의 신축성이나 탄력성을 회복하여 깊은 주름과 피부처짐을 개선시키는 작용을 하게 된다.In addition, the tissue metabolism promoting action of the placenta increases sebum secretion function to normalize the secretion of sebum as a result of reducing or removing the wrinkles. In addition, the placenta contains fibroblast growth factor (FGF), which promotes proliferation and regeneration of fibroblasts, and through its activation increases the amount of collagen, elastin and hyaluronic acid to restore skin elasticity and elasticity. It will work to improve wrinkles and sagging skin.
한편, 돼지 태반은 포유류의 태반 중에서도 인태반의 분자 구조와 놀라울 정도로 흡사한 것으로 밝혀졌는데, 돼지 태반은 프로테인과 각종 영양소 그리고 DNA와 RNA의 중요성분으로서 세포의 차별화와 태아의 발달을 관장하는 Bio-Active cytosin의 근원으로 보고된 바 있다.On the other hand, the pig placenta was found to be remarkably similar to the molecular structure of the placenta in the placenta of the mammal. The pig placenta is an important component of protein, various nutrients, DNA and RNA, and is responsible for the differentiation of cells and the development of the fetus. It has been reported as the source of active cytosin.
그러나, 미용 또는 성형을 목적으로 돼지 태반 유래의 줄기세포를 이용하고자 하는 시도는 미비하였다. However, attempts to use stem cells derived from pig placenta for cosmetic or cosmetic purposes have been insufficient.
이에 본 발명자들은 주름 개선, 피부의 처짐 방지 등의 미용 및 성형 분야에 줄기세포를 이용하고자 예의 노력한 결과, 돼지 태반 조직으로부터 성체 줄기세포를 수득하여 배양한 배양물 및; 상기 돼지 태반 조직 유래 줄기세포에서 추출한 단백질 또는 아미노산을 수득한 다음, 이를 함께 함유하는 조성물이 미용 및 성형에 효과적이라는 것을 확인하고, 본 발명을 완성하게 되었다. Therefore, the present inventors have made efforts to use stem cells in the fields of cosmetic and plastic surgery such as wrinkle improvement, skin sagging prevention, and cultured by obtaining adult stem cells from pig placental tissues; After obtaining the protein or amino acid extracted from the porcine placental tissue-derived stem cells, it was confirmed that the composition containing it together is effective for cosmetic and molding, to complete the present invention.
본 발명의 주된 목적은 (i) 돼지 태반 조직 유래 성체 줄기세포의 배양물; 또는 이에 추가로 (ii) 상기 돼지 태반 조직 유래 성체 줄기세포로부터 추출된 단백질, 또는 상기 단백질의 가수분해물인 펩타이드나 아미노산을 유효성분으로 함유하는 화장료용 조성물을 제공하는데 있다.The main object of the present invention is (i) culture of adult stem cells derived from pig placental tissue; Or in addition to (ii) to provide a cosmetic composition containing a protein extracted from the adult stem cells derived from pig placental tissue, or a peptide or amino acid which is a hydrolyzate of the protein as an active ingredient.
본 발명의 다른 목적은 특정 성분들을 함유하고 있는, 상기 돼지 태반 조직 유래 성체 줄기세포의 배양물을 제조하는 방법을 제공하는데 있다.Another object of the present invention is to provide a method for producing a culture of adult stem cells derived from pig placenta tissue containing specific components.
상기의 목적을 달성하기 위하여, 본 발명은 유효성분으로 돼지 태반 조직 유래 성체 줄기세포 배양물; 또는 상기 돼지 태반 조직 유래 성체 줄기세포로부터 추출한 단백질 또는 아미노산을 추가로 함유하는 기능성 화장료용 조성물을 제공한다.In order to achieve the above object, the present invention is an adult pig stem tissue-derived adult stem cell culture as an active ingredient; Or it provides a functional cosmetic composition further comprising a protein or amino acid extracted from the adult stem cells derived from the placental pig tissue.
또한, 상기 화장료용 조성물의 주요한 유효성분으로 사용되고 있는, 돼지 태반 조직 유래 성체 줄기세포 배양물 및 이를 제조하는 이하의 방법을 제공한다:In addition, the present invention provides a pig placental tissue-derived adult stem cell culture, which is used as a main active ingredient of the cosmetic composition, and the following method of preparing the same:
(a) 돼지 태반조직으로부터 양막을 분리하는 단계;(a) separating the amnion from the pig placental tissue;
(b) 상기 분리된 양막으로부터 성체 줄기세포를 수득하는 단계; 및(b) obtaining adult stem cells from the separated amnion; And
(c) 상기 수득한 성체 줄기세포를 bFGF 가 함유된 배지에서 배양하는 단계.(c) culturing the obtained adult stem cells in a medium containing bFGF.
본 발명의 화장료용 조성물은 도포한 부위의 피부 조직의 재생이 촉진되므로, 특히, 주름 개선 및/또는 노화 방지 효과가 뛰어나다. 따라서, 주름 개선이나 노화 방지용 기능성 화장품 제조에 유용하게 사용될 수 있다. Since the composition for cosmetics of this invention promotes the regeneration of the skin tissue of the apply | coated site | part, it is especially excellent in wrinkle improvement and / or anti-aging effect. Therefore, it can be usefully used for wrinkle improvement or preparation of functional cosmetics for preventing aging.
이하 본 발명에 대하여 구체적으로 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 일 관점에서, 돼지 태반 조직 유래 성체 줄기세포 배양물; 또는 상기 돼지 태반 조직 유래 성체 줄기세포로부터 추출한 단백질 또는 아미노산을 추가로 함유하는 기능성 화장료용 조성물에 관한 것이다.In one aspect, the present invention, porcine placental tissue-derived adult stem cell culture; Or it relates to a functional cosmetic composition further comprising a protein or amino acid extracted from the adult stem cells derived from the placental pig tissue.
본 발명의 기능성 화장료용 조성물은 유효성분으로서 돼지 태반 조직 유래 성체 줄기세포 배양물을 포함한다. Functional cosmetic composition of the present invention comprises an adult stem cell culture derived from pig placental tissue as an active ingredient.
줄기세포(stem cell)란 자기 복제 능력을 가지면서 두 개 이상의 세포로 분화하는 능력을 갖는 세포를 말하며, 성체 줄기세포는 발생과정이 진행되어 배아의 각 장기가 형성되는 단계 혹은 성체단계에서 나타나는 줄기세포를 의미한다.Stem cells are cells that have the capacity of self-replicating and have the ability to differentiate into two or more cells. Adult stem cells are stems that appear during the development or development of each organ of the embryo. It means a cell.
본 발명에서 사용하는 용어 "돼지 태반 조직 유래 성체 줄기 세포"는 돼지의 태반 조직으로부터 분리해 낸 미분화된 성체 줄기세포로서, 본 명세서에서는 축약 하여 "태반 줄기세포"라고 지칭하기도 한다. The term "adult stem cell-derived adult stem cell" used in the present invention is an undifferentiated adult stem cell isolated from the placental tissue of pig, and may be abbreviated herein as "placental stem cell".
본 발명에서 사용하는 용어 "돼지 태반 조직 유래 성체 줄기세포 배양물" 또는 "태반 줄기세포 배양물"은, 상기 돼지 태반 조직 유래 성체 줄기 세포를 특정 성분을 함유하는 배지에 배양시킨 후, 배지를 수거한 다음 세포 데브리스(debris)를 제거하고 남은 브로스(broth)를 의미한다. 결국 상기 배양물에는 배지가 함유하고 있던 주요 성분 및 태반 줄기세포의 분비물이 포함되어 있다고 할 수 있다. The term "adult placental tissue-derived adult stem cell culture" or "placental stem cell culture" used in the present invention, after culturing the adult stem cells derived from porcine placental tissue in a medium containing a specific component, the medium is collected After the removal of the cell debris (debris) refers to the remaining (broth). After all, the culture may be said to contain the secretion of the main components and placental stem cells contained in the medium.
돼지 태반은 회수가 용이하고 많은 양을 확보할 수 있는 장점이 있을 뿐만 아니라, 포유류의 태반 중에서도 인태반의 분자 구조와 놀라울 정도로 흡사한 것으로 밝혀졌다. 돼지 태반은 프로테인과 각종 영양소 그리고 DNA와 RNA의 중요 성분으로서 세포의 차별화와 태아의 발달을 관장하는 Bio-Active cytosin의 근원으로 보고된 바 있다.Porcine placenta has been found to be easy to recover and secure a large amount, as well as surprisingly similar to the molecular structure of the placenta among mammalian placenta. Porcine placenta has been reported to be a source of bio-active cytosin that controls cell differentiation and fetal development as an important component of protein, various nutrients and DNA and RNA.
본 발명의 주요성분인 돼지 태반 조직 유래 성체 줄기세포를 수득하는 방법에 특별한 제한이 있는 것은 아니지만, 예를 들어, 돼지 태반의 양막으로부터 다음의 방법으로 제조할 수 있다: There is no particular limitation on the method for obtaining adult stem cells derived from pig placental tissue, which is the main component of the present invention, but, for example, it can be prepared from the amniotic membrane of pig placenta by the following method:
(a) 돼지 태반조직으로부터 양막을 분리하는 단계;(a) separating the amnion from the pig placental tissue;
(b) 상기 분리된 양막으로부터 성체 줄기세포를 수득하는 단계; 및(b) obtaining adult stem cells from the separated amnion; And
(c) 상기 수득한 성체 줄기세포를 bFGF 가 함유된 배지에서 배양하는 단계.(c) culturing the obtained adult stem cells in a medium containing bFGF.
이하 상기 방법에 대하여 보다 상세히 설명한다. Hereinafter, the method will be described in more detail.
우선, 돼지의 태반을 항생제가 함유된 생리식염수에서 여러 차례 세척과정을 거친 후 태반으로부터 양막, 융모막 및 탈락막을 분리해 낸다. 분리된 양막을 다시 생리식염수(혹은 DPBS)로 2~3회 세척을 하고, 60mm 페트리 디쉬에 옮기고 수술용 가위 등을 이용하여 세절한다. 그 후 콜라게나아제가 함유된 DMEM 배지에 조직을 넣고 37℃에서 1시간 30분 정도 조직분해 과정을 거친다. 이후에 조직이 분해된 정도를 육안으로 확인하고, 100um cell strainer 를 이용하여 필터링을 해주고 걸러진 용액을 원심분리한다.First, the pig's placenta is washed several times in physiological saline containing antibiotics, and then the amniotic membrane, chorion and exfoliating membrane are separated from the placenta. The separated amniotic membrane is washed again two or three times with saline (or DPBS), transferred to a 60mm Petri dish, and cut using surgical scissors. After that, the tissue is placed in DMEM medium containing collagenase and subjected to histolysis process at 37 ° C. for about 1 hour and 30 minutes. After that, the degree of tissue degradation is visually checked, filtered using a 100um cell strainer, and the filtered solution is centrifuged.
돼지 태반조직 유래 펠렛을 원심분리 및 필터링하여 debris를 제거한 후, FBS, NAC 및 ascorbic acid을 함유하는 DMEM 배지에서 배양한다. 부착되지 않은 세포들을 세척하고, FBS, NAC, ascorbic acid, calcium, rEGF, 인슐린 및 hydrocortisone를 함유한 DMEM을 약 2일마다 교체하면서 배양하여 다분화능 중간엽 줄기세포를 분리하여 계대 배양한다. Pellets derived from porcine placenta tissue were centrifuged and filtered to remove debris, and then cultured in DMEM medium containing FBS, NAC and ascorbic acid. Unattached cells are washed and cultured with DMEM containing FBS, NAC, ascorbic acid, calcium, rEGF, insulin, and hydrocortisone every 2 days.
그 다음, Passage 3의 태반조직 유래 다분화능 중간엽 줄기세포를 90% confluency로 배양한 후, NAC, ascorbic acid, calcium, 인슐린 및 hydrocortisone를 함유한 DMEM에서 약 120시간 배양하고, 배지를 수거한 후 원심분리 및 필터링 하여 세포 debris를 제거함으로써 순수한 돼지 태반 줄기세포배양액을 수득한다. Next, the placental tissue-derived multipotent mesenchymal stem cells of Passage 3 were incubated at 90% confluency, incubated for about 120 hours in DMEM containing NAC, ascorbic acid, calcium, insulin and hydrocortisone, and the medium was collected. Centrifugation and filtering remove cell debris to obtain pure porcine placental stem cell culture.
상기 돼지 태반 조직 유래 줄기세포 배양물의 획득에 사용되는 배지로서는 당업계에서 포유류 줄기세포 배양에 적합하다고 알려져있는 통상적인 배지를 사용할 수 있는데, 바람직하게는 DMEM(Dulbecco modified Eagle medium) 또는 aMEM을 사용한다. As a medium used for obtaining the porcine placental tissue-derived stem cell culture, a conventional medium known in the art to be suitable for culturing mammalian stem cells may be used. Preferably, DMEM (Dulbecco modified Eagle medium) or aMEM is used. .
돼지 태반 조직 유래 줄기세포 배양 배지는 줄기세포의 미분화된 표현형의 증식을 촉진하면서 분화는 억제하는 첨가제로 보충될 수 있다. Porcine placental tissue-derived stem cell culture medium may be supplemented with an additive that promotes proliferation of the undifferentiated phenotype of stem cells while inhibiting differentiation.
또한, 배지는 일반적으로, 등장액 중의 중성 완충제(예컨대 인산염 및/또는 고농도 중탄산염) 및 단백질 영양분(예를 들면 혈청, 예컨대 FBS, 혈청 대체물, 알부민, 또는 필수 아미노산 및 비필수 아미노산, 예컨대 글루타민)을 함유할 수 있다. 나아가, 지질(지방산, 콜레스테롤, 혈청의 HDL 또는 LDL 추출물) 및 이 종류의 대부분의 보존액 배지에서 발견되는 기타 성분(예컨대 인슐린 또는 트랜스페린, 뉴클레오시드 또는 뉴클레오티드, 피루빈산염, 임의의 이온화 형태 또는 염인 당원, 예컨대 글루코스, 셀레늄, 글루코코르티코이드, 예컨대 히드로코르티존 및/또는 환원제, 예컨대 β-메르캅토에탄올)을 함유할 수 있다.In addition, the medium generally contains neutral buffers (such as phosphates and / or high concentrations of bicarbonate) and protein nutrients (such as serum, such as FBS, serum substitutes, albumin, or essential and non-essential amino acids, such as glutamine) in isotonic solutions. can do. Furthermore, lipids (fatty acids, cholesterol, HDL or LDL extracts of serum) and other components found in most preservative media of this kind (such as insulin or transferrin, nucleosides or nucleotides, pyruvate salts, any ionized form or salt) Sugar sources such as glucose, selenium, glucocorticoids such as hydrocortisone and / or reducing agents such as β-mercaptoethanol.
또한, 배지는 세포가 서로 유착하거나, 용기벽에 유착하거나, 너무 큰 다발을 형성하는 것을 방지할 목적으로, 항응집제 (anti-clumping agent), 예컨대 Invitrogen이 판매하는 것들(Cat # 0010057AE)을 포함하는 것이 유익할 수 있다.The medium also contains anti-clumping agents, such as those sold by Invitrogen (Cat # 0010057AE), with the aim of preventing the cells from adhering to each other, adhering to the vessel wall, or forming too large a bundle. It can be beneficial to do so.
그 중에서도, 하기의 1이상의 추가의 첨가제를 사용하는 것이 유리할 수 있다:Among other things, it may be advantageous to use one or more additional additives as follows:
ㆍ 줄기 세포 인자(SCF, Steel 인자), c-키트를 이량화하는 다른 리간드 또는 항체, 및 동일한 신호 전달 경로의 다른 활성제Stem cell factor (SCF, Steel factor), other ligands or antibodies that dimerize the c-kit, and other active agents of the same signal transduction pathway
ㆍ 다른 티로신 키나아제 관련 수용체, 예컨대 혈소판-유도된 성장 인 자(Platelet-Derived Growth Factor; PDGF), 대식세포 콜로니-자극 인자, Flt-3 리간드 및 혈관 내피 성장 인자(Vascular Endothelial Growth Factor; VEGF)의 수용체를 위한 리간드Of other tyrosine kinase related receptors, such as platelet-Derived Growth Factor (PDGF), macrophage colony-stimulating factor, Flt-3 ligand and Vascular Endothelial Growth Factor (VEGF) Ligands for Receptors
ㆍ 환형 AMP 농도를 높이는 인자, 예컨대 포르스콜린Factors that increase cyclic AMP concentrations, such as forskolin
ㆍ gp130을 유도하는 인자, 예컨대 LIF 또는 Oncostatin-MFactors inducing gp130, such as LIF or Oncostatin-M
ㆍ 조혈모 성장 인자, 예컨대 트롬보포이에틴(TPO)Hematopoietic growth factor such as thrombopoietin (TPO)
ㆍ 변형성 성장 인자, 예컨대 TGFβ1Modifying growth factors such as TGFβ1
ㆍ 다른 성장 인자, 예컨대 표피 성장 인자(EGF)Other growth factors such as epidermal growth factor (EGF)
ㆍ 뉴로트로핀, 예컨대 CNTFNeurotropins, such as CNTF
ㆍ N-acetyl-L-cysteine (NAC)N-acetyl-L-cysteine (NAC)
ㆍ HydrocortisoneHydrocortisone
ㆍ Ascrobic Acidㆍ Ascrobic Acid
특히, 본 발명의 돼지 태반 조직 유래 줄기세포 배양을 위한 배지는 bFGF를 함유하는 것이 바람직하다..In particular, the medium for culturing pig placental tissue-derived stem cells of the present invention preferably contains bFGF.
본 발명의 돼지 태반 줄기세포 배양물은 상기 배지 성분 이외에도, 함유되어 있는 돼지 태반 줄기세포가 고농도로 분비하는 이하와 같은 단백질 성분을 함께 함유하고 있을 수 있다: Porcine placental stem cell culture of the present invention, in addition to the medium component, may contain the following protein components secreted in high concentration by the contained pig placental stem cells:
IGF-1(820.8pg/ml)IGF-1 (820.8 pg / ml)
fibronectin(1295.7pg/ml)fibronectin (1295.7 pg / ml)
PDGF(417.17pg/ml)PDGF (417.17 pg / ml)
VEGF(647.43pg/ml)VEGF (647.43 pg / ml)
Procollagen(598.4ng/ml). Procollagen (598.4 ng / ml).
IGF-I (Insunlin-like Growth Factor-1) 인슐린 유사 성장인자는 70개의 아미노산으로 형성된 7.6kD의 단일 사슬 폴리펩타이드 호르몬으로, 성장호르몬에 반응하여 뼈, 근육, 신경 조직을 형성하고 다양한 조직에서 세포의 분화를 자극함으로서 손상된 세포를 재생시키고 성장을 촉진하는 역할을 한다. VEGF (vascular endothelial growth factor) 혈관내피 성장인자는 모세혈관에서 혈장단백질을 투과를 증가시키는 cytokine으로 세포의 분열과 이동을 촉진시키며 세포의 재구성을 일으키는 단백질분해효소를 유도하는 기능을 가진다. 또한 세포소멸의 억제를 통해 새로 형성된 혈관의 생존을 유지시키며 신경세포의 항원 제공 억제하여 면역조절을 담당하여 세포의 생장 및 분열을 유도한다. 혈관세포의 이동을 촉진시켜 새로운 세포의 발생 및 분화를 촉진시켜 피부의 구성을 증가시킨다. Platelet-derived growth factor (PDGF) 는 세포 성장 및 분열을 조절하는 성장인자로서 특히 혈관 형성에 중요한 역할을 한다. 파이브로넥틴은 부착성 당단백질로서 창상기질 위로 상피세포의 이동을 촉진시킴으로 상처치유를 돕는다. 파이브로넥틴은 피부 구성성분 중의 하나로 피부 재생 및 탄력유지에 관여한다. Procollagen은 피부의 진피층을 구성하는 단백질 성분으로 수분과 결합해 피부의 촉촉함과 탄력을 유지하는 기 능을 한다.IGF-I (Insunlin-like Growth Factor-1) Insulin-like growth factor (IGF-I) is a 7.6-kD single-chain polypeptide hormone formed of 70 amino acids that forms bone, muscle, and nerve tissue in response to growth hormones. It stimulates the differentiation of cells, thereby regenerating damaged cells and promoting growth. VEGF (vascular endothelial growth factor) Vascular endothelial growth factor is a cytokine that increases the permeation of plasma proteins in capillaries and promotes cell division and migration and induces protease that causes cell reorganization. In addition, by suppressing cell death, the survival of newly formed blood vessels is maintained, and the antigen supply of neurons is suppressed to induce immune regulation to induce cell growth and division. It promotes the migration of vascular cells to promote the development and differentiation of new cells, thereby increasing the composition of the skin. Platelet-derived growth factor (PDGF) is a growth factor that regulates cell growth and division and plays an important role in blood vessel formation. Fibronectin is an adherent glycoprotein that aids in wound healing by promoting the migration of epithelial cells over the wound matrix. Fibronectin is one of the skin components involved in skin regeneration and elasticity. Procollagen is a protein that makes up the dermal layer of the skin and combines with moisture to maintain the skin's moisture and elasticity.
또한, 본 발명의 기능성 화장료용 조성물은 그 유효성분의 하나로서 상기 돼지 태반 조직 유래 성체 줄기세포 배양물 외에도, 돼지 태반 조직 유래 성체 줄기세포로부터 추출된 단백질; 또는 상기 단백질의 가수분해물인 펩타이드나 아미노산을 추가로 포함한다. 이는 돼지 태반 줄기세포의 정제물이라고도 할 수 있다.In addition, the functional cosmetic composition of the present invention as one of its active ingredients, in addition to the adult stem cell culture derived from pig placental tissue, protein extracted from porcine placental tissue derived adult stem cells; Or a peptide or amino acid which is a hydrolyzate of the protein. It can also be called a purified product of porcine placental stem cells.
본 발명에서, 돼지 태반 조직 유래 성체 줄기세포로부터 추출한 단백질 또는 아미노산을, 예를 들어, 이하와 같은 방법으로 획득할 수 있다. In the present invention, a protein or amino acid extracted from porcine placental tissue-derived adult stem cells, for example, can be obtained by the following method.
이 방법은 돼지 태반 줄기세포를 구성하는 단백질을 염 침전, 투석하여 활성을 유지한 상태로 제조하는 방법에 관한 것이다. 돼지 태반 줄기세포를 세척하여 돼지 태반 줄기세포 배양액의 잔류성분이 남지 않도록 한 후, 돼지 태반 줄기세포를 파쇄하고 Ammonium Sulfate를 상등액에 조금씩 첨가하여 단백질을 침전시켜 단백질 Pellet을 회수한다. 그 다음, 불순물을 제거하는 수단으로 투석을 실시한다. 상기 투석이 끝나면 단백질을 회수하여 원심분리하고, 상등액을 회수하여 동결건조함으로써 돼지 태반 줄기세포의 단백질 성분을 추출한다. This method relates to a method of preparing the protein constituting the porcine placental stem cells in salt precipitation and dialysis to maintain the activity. After washing the pig placental stem cells to ensure that no residual components of the pig placental stem cell cultures remain, the pig placental stem cells are crushed and Ammonium Sulfate is added to the supernatant little by little to precipitate the protein to recover the protein pellets. Then, dialysis is performed by means of removing impurities. After the dialysis, the protein is recovered by centrifugation, the supernatant is recovered, and lyophilized to extract protein components of porcine placental stem cells.
그리고, 상기 돼지 태반 줄기세포를 구성하는 단백질을 가수분해하여 저분자의 아미노산과 펩타이드를 제조할 수 있다. 돼지 태반 줄기세포배양액의 잔류성분이 남지 않도록 하여 수득한 돼지 태반 줄기세포에 프로테아제를 처리하여 교반하며 효소분해를 실시한다. 이 과정이 끝나면 돼지 태반 줄기세포를 구성하는 단백질 은 펩타이드와 아미노산 상태로 변하게 된다. In addition, low-molecular amino acids and peptides can be prepared by hydrolyzing the proteins constituting the porcine placental stem cells. Porcine placental stem cells obtained by leaving no residual components of the pig placental stem cell culture solution are treated with a protease, stirred and subjected to enzymatic digestion. After this process, the proteins that make up the porcine placental stem cells are converted into peptide and amino acid states.
상기의 방법으로 수득한 동결 건조된 돼지 태반 줄기세포 추출 단백질, 또는 저분자 펩타이드 또는 아미노산을, 앞서 설명한 돼지 태반 줄기세포 배양액으로 부유하여 본 발명의 화장료용 조성물을 만들 수 있다. The freeze-dried porcine placental stem cell extract protein, or a low molecular peptide or amino acid obtained by the above method may be suspended in the above-described porcine placental stem cell culture solution to make a cosmetic composition of the present invention.
또한, 상기 성분 이외에도 돼지 태반 줄기세포 배양액에 존재하는 성분 중 일부의 함량을 증가시키기 위해(예를 들면, 프로콜라젠), 특정 추출물, 예를 들어 감초 등을 첨가하여 사용할 수 있다. In addition to the above components, in order to increase the content of some of the components present in porcine placental stem cell culture (for example, procollagen), it may be used by adding a specific extract, for example licorice.
본 발명의 상기 조성물을 유효 성분으로 하여, 기능성 화장료를 제조하여 사용할 수 있다.Using the said composition of this invention as an active ingredient, a functional cosmetics can be manufactured and used.
이 때, 화장료로는 화장수, 유액, 크림, 젤, 미용액(엣센스), 팩 화장료 등을 들 수 있다. 본 발명의 조성물을 유효량 포함하는 화장료는 도포한 부위의 피부 조직의 재생이 촉진되므로, 특히, 주름 개선 및/또는 노화 방지 효과에 의해 젊고 건강한 피부를 유지할 수 있다.At this time, cosmetics include lotion, milky lotion, cream, gel, essence (essence), pack cosmetics, and the like. Cosmetics containing an effective amount of the composition of the present invention is promoted the regeneration of the skin tissue of the applied site, in particular, it is possible to maintain a young and healthy skin by the wrinkle improvement and / or anti-aging effect.
"화장수"는 일반적으로 피부를 청결하고 건강하게 유지하기 위해서 피부 표면에 도포하는 투명 액상의 화장료이다. 화장수의 기본 기능은 피부의 각질층에 수분이나 보습 성분을 보급하는 것이며, 화장수는 피부를 유연하게 하는 기능 등도 있다. 화장수에는 물에 녹기 어려운 물질을 용해시키고 안정시켜 외관을 투명상태로 한 것, 마이크로에멀젼이나 리피드나노스피어를 이용한 투명 또는 반투명의 것, 수%의 유분을 O/W형(오일인워터형), W/O형 또는 W/S형으로 유화한 불투명 화장수, 및 수용성 고분자를 배합한 것 등을 들 수 있다. 본 발명의 트리트먼트 방법으로는 공지의“화장수”를 그 용도 등에 따라 적절히 이용할 수 있다."Cosmetic water" is generally a clear liquid cosmetic applied to the skin surface in order to keep the skin clean and healthy. The basic function of the lotion is to supply moisture or moisturizing ingredients to the stratum corneum of the skin, and the lotion also has the function of softening the skin. Dissolves and stabilizes substances that are difficult to dissolve in water to make the appearance transparent. Transparent or translucent using microemulsion or lipid nanosphere, O / W type (oil in water type), The opaque lotion emulsified by W / O type or W / S type | mold, the thing which mix | blended the water-soluble polymer, etc. are mentioned. As the treatment method of the present invention, known "cosmetics" can be appropriately used depending on the purpose thereof.
화장수에는 본 발명의 조성물 이외, 예를 들어 이하의 성분이 포함되어 있다. 즉, 화장수에는 이온 교환수 등, 수용성 성분을 용해하여, 각질층에 수분을 공급하기 위한 정제수; 에탄올, 프로파놀 등, 유용성 성분을 용해하고, 살균하여, 청량감을 주기 위한 알코올; 글리세린, PEG, 히알론산 등, 각질층의 보습을 위한 보습제; 에스테르유, 식물유 등, 보습성이나 사용감의 향상을 위한 에모리엔트제(수분이 증발하는 것을 막는 기름 성분); 폴리옥시에틸렌오레일알코올 에테르 등, 원료 성분을 가용화하기 위한 가용화제; 구연산, 유산, 아미노산류 등, 제품의 pH를 조정하기 위한 완충제; 바니린, 오렌지플래이버, 레몬플래이버, 밀크후레이버게라니올,리나롤 등 향을 부가하기 위한 향료; 메틸파라벤, 페녹시 에탄올 등의 미생물을 억제하여, 부패를 방지하기위한 방부제; 착색하기 위한 착색제; 금속 이온 봉쇄제, 자외선 흡수제 등, 퇴색이나 변색을 방지하기 위한 퇴색 방지제; 및 수렴제, 살균제, 활력제, 소염제, 또는 미백제 등의 약제가, 2종 이상 혼합되어 포함되어 있을 수 있다.In addition to the composition of this invention, lotion contains the following components, for example. Namely, purified water for dissolving water-soluble components such as ion-exchanged water and supplying water to the stratum corneum; alcohol for dissolving and sterilizing oil-soluble components such as ethanol and propanol to give a refreshing feeling; glycerin, PEG, hyal Moisturizers for moisturizing the stratum corneum, such as lonic acid; emollients (oil components that prevent moisture from evaporating); moisturizing agents such as ester oils and vegetable oils; and raw material components such as polyoxyethylene oleyl alcohol ethers. Solubilizer for solubilizing; buffering agent for adjusting pH of products such as citric acid, lactic acid, amino acids, etc .; for adding flavors such as vanillin, orange flavor, lemon flavor, milk flavor geraniol, linarol Flavoring agent; Antiseptic for inhibiting microorganisms such as methylparaben and phenoxy ethanol to prevent decay; Colorant for coloring; Metal ion sequestrant, Ultraviolet ray Hand et al., Fading agent to prevent fading or discoloration; a drug, such as astringents and, fungicides, energetic agents, anti-inflammatory agents, or whitening agent, may contain a mixture of two or more.
화장수의 전체량을 100 중량%로 했을 경우에, 본 발명의 조성물을 0.00 ~ 20 중량%, 바람직하게 는 0.01 ~ 10 중량%, 보다 바람직하게는 0.1 ~ 5 중량% 정도 포함할 수 있다.When the total amount of the lotion is 100% by weight, the composition of the present invention may contain 0.00 to 20% by weight, preferably 0.01 to 10% by weight, more preferably about 0.1 to 5% by weight.
"유액"은 화장수와 크림의 중간적인 성질을 가지는 것이며, 일반적으로는 유 동성이 있는 에멀젼이다. 유액은 주로 피부의 모이스처 밸런스, 보습성 및 유연성을 유지하기 위해서, 피부에 수분, 보습제 및 유분 등을 공급하기 위해 이용되는 화장료이다. 유액에 포함되는 성분은 후술의 크림에 포함되는 성분과 유사하지만, 유액은 유동성이 있으므로, 크림에 비해 고형 유분이나 납류의 양이 적다. 본 발명의 트리트먼트 방법에서는 공지의“유액”을, 그 용도 등에 따라 적절히 이용할 수 있다."Emulsion" is an intermediate between a lotion and a cream and is generally a fluid emulsion. Latex is a cosmetic mainly used to supply moisture, moisturizer, oil, etc. to the skin in order to maintain the skin's moisture balance, moisturizing and flexibility. The components contained in the milky milk are similar to the ingredients contained in the creams described later. However, since the milky milk is fluid, the amount of solid oil and lead is less than that of the creams. In the treatment method of this invention, a well-known "oil emulsion" can be used suitably according to the use etc.
유액은 본 발명의 조성물을 예를 들어, 0.001 ~ 20 중량%, 바람직하게는 0.01 ~ 10 중량%, 보다 바람직하게는 0.1 ~ 5 중량% 정도 포함할 수 있다.The emulsion may contain, for example, 0.001 to 20% by weight, preferably 0.01 to 10% by weight, more preferably about 0.1 to 5% by weight of the composition of the present invention.
"크림"은 물과 기름처럼 서로 서로 섞이지 않는 액체의 한쪽을 분산상으로서, 다른쪽 분산매에 안정적인 상태로 분산시킨 에멀젼의 일종이다. 본 발명의 트리트먼트 방법에서는 공지의“크림”을, 그 용도 등에 따라 적절히 이용할 수 있다. 이러한 크림으로는 피부의 보습이나 유연을 위한 에모리엔트 크림, 혈행을 촉진하기 위한 맛사지 크림, 피부의 세정을 위한 클렌징 크림, 탈모를 위한 헤어 리무버, 냄새제거를 위한 데오도란트 크림, 및 각질연화를 위한 각질연화 크림 등을 들 수 있다."Cream" is a type of emulsion in which one side of a liquid that does not mix with each other, such as water and oil, is dispersed in a stable state in the other dispersion medium. In the treatment method of this invention, well-known "cream" can be used suitably according to the use etc. These creams include emollient creams for moisturizing and softening of the skin, massage creams for promoting blood circulation, cleansing creams for cleansing the skin, hair removers for hair loss, deodorant creams for odor removal, and keratin softening. Keratin softening cream and the like.
크림에는 예를 들면 아래의 성분이 포함되어 있다. 즉, 크림에 포함되는 수상성분으로는 이온 교환수 등의 정제수; 에탄올, 프로파놀 등, 유용성 성분을 용해하고 살균하여, 청량감을 주기 위한 알코올; 글리세린, PEG,히알론산 등, 각질층의 보습을 위한 보습제; 및 퀸스시드, 펙틴, 셀룰로오스 유도체 등의 점액질을 들 수 있다. 크림에 포함되는 유상성분으로는 스쿠알렌, 유동 파라핀, 바셀린, 고 형 파라핀 등의 탄화수소; 올리브유, 아몬드유, 카카오지방, 피마자유 등의 유지; 밀납, 라놀린, 호호바유 등의 납; 스테아린산, 올레인산, 팔미틴산 등의 지방산, 세타놀, 스테아릴알코올 등의 고급 알코올; IPM, 글리세린트리에스텔, 펜타에리스리톨테트라에스테르 등의 에스테르; 및 폴리실록산류 등의 실리콘유 등을 들 수 있다. 크림에 포함되는 각 성분의 양은 그 종류나 용도에 따라 크게 다르지만, 본 발명의 트리트먼트 방법에서는 공지의 것을 적절히 이용하면 된다.The cream contains, for example, the following ingredients: That is, the aqueous phase components included in the cream include purified water such as ion-exchanged water; alcohols for dissolving and sterilizing oil-soluble components such as ethanol and propanol to give a refreshing feeling; for moisturizing the stratum corneum, such as glycerin, PEG and hyaluronic acid. Humectants; and mucus such as queen's seed, pectin, cellulose derivatives, and the like. Oil components included in the cream include hydrocarbons such as squalene, liquid paraffin, petrolatum and solid paraffin; oils such as olive oil, almond oil, cacao fat and castor oil; lead such as beeswax, lanolin, jojoba oil; stearic acid, oleic acid, Higher alcohols such as fatty acids such as palmitic acid, cetanol and stearyl alcohol, esters such as IPM, glycerin triester, pentaerythritol tetraester, silicone oils such as polysiloxanes, and the like. Although the quantity of each component contained in cream differs greatly by the kind and use, a well-known thing may be used suitably in the treatment method of this invention.
크림은 본 발명의 조성물을 0.001 ~ 20 중량%, 바람직하게는 0.01 ~ 10 중량%, 보다 바람직하게는 0.1 ~ 5 중량% 정도 포함하면 된다.The cream may contain 0.001 to 20% by weight, preferably 0.01 to 10% by weight, more preferably about 0.1 to 5% by weight of the composition of the present invention.
젤은 겔 또는 졸 등 외관 상태가 균일하고 투명에서부터 반투명의 화장료이다. 본 발명의 트리트먼트 방법으로는 공지의“젤”을, 그 용도 등에 따라 적절히 이용할 수 있다. 피부에 수분을 보급하고, 보습하기 위한 수성젤, 피부의 보습을 유지하고, 유분을 보급하기 위한 유성 젤, 혈행을 촉진하기 위한 맛사지 용수성 젤, 및 세정용의 젤 등을 들 수 있다. 젤로는 카르복시비닐폴리머, 메틸셀룰로오스 등의 수용성 고분자를 포함한 겔화제를 이용해 제조하는 것을 들 수 있다. 또한, 겔상의 맛사지 오일을 이용해도 된다.Gels are cosmetics with a uniform appearance, such as gels or sols, and are transparent to translucent cosmetics. As the treatment method of the present invention, a known "gel" can be appropriately used depending on the purpose thereof. And an aqueous gel for replenishing and moisturizing the skin, an oily gel for maintaining the moisture of the skin, and replenishing oil, a water-soluble gel for promoting blood circulation, and a gel for washing. The gel may be prepared by using a gelling agent containing a water-soluble polymer such as carboxyvinyl polymer and methyl cellulose. Moreover, you may use gel massage oil.
"미용액(엣센스)"은 기본적으로는 상기의 화장수, 유액 및 크림과 같은 성분이며, 유제, 가용화제, 보습제, 물, 및 그 외의 약제를 포함하는 것을 들 수 있다. 본 발명의 트리트먼트 방법에서의 공지의 미용액을 이용할 수 있다. 미용액에 포함되는 유제로는 올리브유, 동백유, 참기름, 해바라기유, 스위트아몬드유, 호호바유 등의 천연 식물유;카프린산, 미리스틴산, 올레인산, 이소스테아린산 등의 지방산 의 디글리세린 에스테르 또는 트리글리세린에스테르 등을 들 수 있다. 이러한 유제는 주로 에모리엔트제로서 기능한다. 미용액에 포함되는 가용화제로는 글리세린지방산에스테르, 폴리글리세린지방산에스테르,솔비탄지방산에스테르, 및 모노스테아린산프로필렌 글리콜 등의 프로필렌글리콜지방산에스테르류, 글리세린,디글리세린, 에틸렌글리콜, 프로필렌글리콜, 1, 3-부틸렌글리콜 등의 다가(多價)알코올 등을 들 수 있다. 한편, 가용화제는 글리세린 또는 디글리세린을 사용하는 것과, 맛사지에 있어서 온열 효과를 높이므로 바람직하다. 미용액에 포함되는 물로는 증류수, 및 탈이온수를 들 수 있다. 기타 약제로는 살균제, 방부제, 비타민류,식물로부터의 천연 엑기스, 색소, 향료 등을 들 수 있다."Cosmetics (essences)" is basically a component such as the lotion, emulsion and cream described above, and includes those containing an emulsion, a solubilizer, a moisturizer, water, and other drugs. The well-known cosmetic liquid in the treatment method of this invention can be used. Emulsions included in the cosmetic liquid include natural vegetable oils such as olive oil, camellia oil, sesame oil, sunflower oil, sweet almond oil and jojoba oil; diglycerin esters or triglycerin esters of fatty acids such as caprinic acid, myristic acid, oleic acid and isostearic acid. Etc. can be mentioned. Such emulsions mainly function as emollients. Solubilizers included in the cosmetic liquid include propylene glycol fatty acid esters such as glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, and propylene glycol monostearate, glycerin, diglycerin, ethylene glycol, propylene glycol, 1,3-butyl Polyhydric alcohols, such as len glycol, etc. are mentioned. On the other hand, a solubilizer is preferable because it uses glycerin or diglycerol, and since a thermal effect is heightened in a massage. Distilled water and deionized water are mentioned as water contained in a cosmetic liquid. Other pharmaceutical agents include fungicides, preservatives, vitamins, natural extracts from plants, pigments and flavorings.
미용액을 구성하는 각 성분의 양은 공지 성분량을 채용할 수 있다. 예를 들면, 미용액 전체량을 100 중량%로 했을 경우에, 본 발명의 조성물을 0.001 ~ 20 중량%, 바람직하게는 0.01 ~ 10 중량%, 보다 바람직하게는 0.1 ~ 5 중량% 정도 포함하면 된다. 또한, 유제 65 ~ 90 중량%,가용화제 1 ~ 10 중량%, 보습제 1 ~ 30 중량%, 기타 약제 0 ~ 10 중량% 정도, 및 나머지는 물을 들 수 있다. 미용액은 공지 제조 방법에 따라 제조하면 된다.The quantity of each component which comprises a cosmetic liquid can employ | adopt a well-known component amount. For example, when the total amount of the cosmetic liquid is 100% by weight, the composition of the present invention may contain 0.001 to 20% by weight, preferably 0.01 to 10% by weight, more preferably about 0.1 to 5% by weight. Moreover, 65 to 90 weight% of an emulsion, 1 to 10 weight% of a solubilizer, 1 to 30 weight% of a moisturizer, about 0 to 10 weight% of other drugs, and the remainder are water. What is necessary is just to manufacture a cosmetic liquid according to a well-known manufacturing method.
"팩 화장료"는 피부의 보습, 혈행 촉진, 청정을 목적으로, 대상 부위에 도포하는 것이다. 팩 화장료에는 일반적으로, 젤리상, 페이스트상, 분말상, 크림상 액상 등을 들 수 있으며, 안면에 도포하여 피막을 형성시킨 후 박리하는 것이나,도포 후에 닦아내는 것, 도포 후 씻어내는 것 등이 있다. 분말상의 것은 사용시에 물 등에 균일하게 녹이던지 현탁해서 사용한다. 또한, 아이마스크 등의 형상의 부직포나 콜라겐 시트에 화장료를 함침시킨 팩 화장료나 상기 부직포 등을 사용시에 화장료에 침지해 이용하는 팩 화장료 등이어도 된다."Pack cosmetic" is to apply to the target site for the purpose of moisturizing the skin, promoting blood circulation, and cleansing. Pack cosmetics generally include a jelly, paste, powder, creamy liquid, and the like, and may be applied to the face to form a film and then peeled off, wiped off after application, or washed off after application. . Powdered materials are used by dissolving or suspending uniformly in water or the like during use. Moreover, the pack cosmetics which impregnated cosmetics to the nonwoven fabric and collagen sheets of shapes, such as an eye mask, the pack cosmetics etc. which are immersed in cosmetics at the time of use may be sufficient.
팩 화장료는 본 발명의 조성물을 0.001 ~ 20 중량%, 바람직하게는 0.01 ~ 10 중량%, 보다 바람직하게는 0.1 ~ 5 중량%, 가장 바람직하게는 0.2 ~ 2 중량% 정도 포함하면 된다. 기타 조성은 팩 화장료에 이용되는 공지 조성으로 하면 되고, 공지 제조 방법에 따라 제조하면 된다.Pack cosmetics may contain 0.001 to 20% by weight, preferably 0.01 to 10% by weight, more preferably 0.1 to 5% by weight, most preferably about 0.2 to 2% by weight of the composition of the present invention. The other composition may be a known composition used for a pack cosmetic, and may be prepared according to a known production method.
그러나, 상기 설명에 제한받지 않고, 상기의 화장료는 각각이 통상 이용되는 방법에 따라 사용될 수 있다. However, without being limited to the above description, the above cosmetics may be used according to the method in which each is commonly used.
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090015330A KR20100096447A (en) | 2009-02-24 | 2009-02-24 | Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090015330A KR20100096447A (en) | 2009-02-24 | 2009-02-24 | Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20100096447A true KR20100096447A (en) | 2010-09-02 |
Family
ID=43003972
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020090015330A KR20100096447A (en) | 2009-02-24 | 2009-02-24 | Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR20100096447A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105963162A (en) * | 2016-07-02 | 2016-09-28 | 广东万海细胞生物科技有限公司 | Deep restoring mask and preparation method thereof |
CN105963163A (en) * | 2016-07-02 | 2016-09-28 | 广东万海细胞生物科技有限公司 | Wrinkle-resisting and freckle-removing essence and preparation method thereof |
CN108186536A (en) * | 2018-03-14 | 2018-06-22 | 河南奥比特生物技术有限公司 | A kind of skin care item and preparation method thereof |
KR20190003303A (en) * | 2017-12-26 | 2019-01-09 | (주) 차바이오에프앤씨 | Composition for anti-aging containing epidermal stem cell conditioned medium and use thereof |
KR20190066888A (en) * | 2017-12-06 | 2019-06-14 | 이정복 | Medium composition for cell proliferation, skin regeneration and wrinkle improvement comprising conditioned medium of pluripotent stem cells, neural stem cells and embryonic fibroblasts isolated from an egg of a bird |
WO2021045705A3 (en) * | 2019-07-02 | 2021-06-03 | AKSOY, Artun | Repair cream with human stem cell |
-
2009
- 2009-02-24 KR KR1020090015330A patent/KR20100096447A/en not_active Application Discontinuation
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105963162A (en) * | 2016-07-02 | 2016-09-28 | 广东万海细胞生物科技有限公司 | Deep restoring mask and preparation method thereof |
CN105963163A (en) * | 2016-07-02 | 2016-09-28 | 广东万海细胞生物科技有限公司 | Wrinkle-resisting and freckle-removing essence and preparation method thereof |
CN105963163B (en) * | 2016-07-02 | 2018-12-14 | 广东万海细胞生物科技有限公司 | A kind of dewrinkling spot-eliminating Essence and preparation method thereof |
KR20190066888A (en) * | 2017-12-06 | 2019-06-14 | 이정복 | Medium composition for cell proliferation, skin regeneration and wrinkle improvement comprising conditioned medium of pluripotent stem cells, neural stem cells and embryonic fibroblasts isolated from an egg of a bird |
US11564951B2 (en) | 2017-12-06 | 2023-01-31 | Jung Bok Lee | Medium composition for cell proliferation, skin regeneration, and wrinkle improvement comprising, as active ingredient, conditioned medium of pluripotent stem cells, neural stem cells, or embryonic fibroblasts which are isolated from avian eggs |
KR20190003303A (en) * | 2017-12-26 | 2019-01-09 | (주) 차바이오에프앤씨 | Composition for anti-aging containing epidermal stem cell conditioned medium and use thereof |
CN108186536A (en) * | 2018-03-14 | 2018-06-22 | 河南奥比特生物技术有限公司 | A kind of skin care item and preparation method thereof |
WO2021045705A3 (en) * | 2019-07-02 | 2021-06-03 | AKSOY, Artun | Repair cream with human stem cell |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101678621B1 (en) | Composition comprising spicules for skin care | |
JP5981947B2 (en) | Skin cream | |
DE60024612T2 (en) | METHOD FOR PRODUCING CONDITIONED CELL CULTURE MEDIUM COMPOSITIONS | |
KR101894229B1 (en) | Functional composition comprising deer antlers derived stem cell culture medium | |
KR101885501B1 (en) | Functional composition comprising deer antlers derived stem cell culture medium | |
US20110177015A1 (en) | Skin and hair care using extract from conditioned medium cultured by mesenchymal stem cells and other regenerative cells | |
US20120315259A1 (en) | Method and composition for skin care comprising cord blood serum or plasma or components thereof | |
JP2013224323A (en) | Skin care composition and treatment | |
KR20100008763A (en) | Cosmetic composition comprising matrials cultured multipotent stem cells derived from adipose tissue and proteins extracted therefrom | |
WO2005065269A2 (en) | Compositions and method for decreasing the appearance of skin wrinkles | |
CN112336749A (en) | Stem cell exosome microneedle patch for removing freckles and wrinkles and preparation method thereof | |
US20200230172A1 (en) | Stem cell conditioned media for clinical and cosmetic applications | |
KR20180134468A (en) | Cosmetic compositions comprising spicule, marine collagen and deep ocean water and their preparation | |
KR20100096447A (en) | Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom | |
US20170035687A1 (en) | Skin treatment formulations | |
JP2016515642A (en) | Synergistic combination of alanine-glutamine, hyaluronic acid and oat extract and its use in compositions for wound treatment and skin lesion repair | |
JP6159183B2 (en) | Stem cell-derived growth factor production promoter | |
WO2022071610A1 (en) | Composition for improving and/or ameliorating health status of skin and method for producing the same | |
JP2014214094A (en) | Cosmetic composition containing liposome including culture solution extract of stem cells derived from human | |
JP6200713B2 (en) | Stem cell-derived growth factor production promoter | |
RU2144815C1 (en) | Method of production of regenerating cosmetic cream for skin care | |
KR101570808B1 (en) | Hair Care Composition Containing Human Adipocyte Conditioned Media Extracts and Pleuropterus Multiflorus Stem Cell Culture Extracts for Hair Growth | |
KR20120058829A (en) | Cosmetic composition for promoting the stemness of adipose-derived stem cell and proliferating skin cell containing sequoia callus cell extracts | |
US20230330183A1 (en) | Stem cell conditioned media for clinical and cosmetic applications | |
JP7202610B2 (en) | Stem cell undifferentiated state maintenance agent and growth promoter |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20090224 |
|
PG1501 | Laying open of application | ||
A201 | Request for examination | ||
PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20140221 Comment text: Request for Examination of Application Patent event code: PA02011R01I Patent event date: 20090224 Comment text: Patent Application |
|
E902 | Notification of reason for refusal | ||
PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20150521 Patent event code: PE09021S01D |
|
E601 | Decision to refuse application | ||
PE0601 | Decision on rejection of patent |
Patent event date: 20150818 Comment text: Decision to Refuse Application Patent event code: PE06012S01D Patent event date: 20150521 Comment text: Notification of reason for refusal Patent event code: PE06011S01I |