KR20090030526A - Collagen formation promoting composition and cosmetics using the same - Google Patents

Abstract

The present invention relates to a composition for promoting collagen formation, including franginidin and ginsenoside Re. The composition of the present invention has an excellent collagen formation promoting ability, in particular, even when a small amount of the active ingredient has the advantage that the above-mentioned efficacy is exerted. Accordingly, the composition of the present invention is excellent in the ability to suppress and improve the occurrence of wrinkles associated with collagen reduction, and also excellent in the healing effect of the wound on the skin. In addition, since the composition of the present invention exhibits excellent efficacy even with a small amount of active ingredients, it can be used in various various formulations without limitations such as solubility in solvents when applied to cosmetics, for example, and thus has a wide range of application. Has

Description

Composition for promoting collagen formation and cosmetics using the same {Compositions for promotion of collagen synthesis and cosmetics using the same}

The present invention relates to a composition for promoting collagen formation comprising franginidin and ginsenoside Re and cosmetics comprising the same.

Collagen, a major component of the extracellular matrix, is present in extracellular epilepsy as a major substrate protein produced by skin fibroblasts. The collagen is an important protein that accounts for about 30% of the total weight of biological proteins and has a solid triple helix structure. Collagen forms most of the organic material of the skin, tendons, bones and teeth, especially in bones and skin (dermis), and in other structures, mainly as fibrous inclusions.

Collagen is a relatively weak immunogen, one of which is the shielding of latent antigenic determinants by the spiral structure of collagen. The helix structure also makes collagen resistant to proteolysis. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation (in organic growth or wound healing) (Van der Rest et al. , Ann NY Acad Sci, 1990). Such collagen decreases due to age and photoaging by UV irradiation, which is known to be closely related to wrinkle formation of the skin (Arthur K. Balin et al., Aging and the skin, 1989).

Collagen also plays an important role in wound healing, and thus, promoting collagen synthesis in the damaged epithelium can quickly restore the wound without scars.

Accordingly, in order to take advantage of the skin moisturizing effect and wound healing effect of collagen, a number of products incorporating collagen in an external skin composition such as cosmetics or ointment have been released. These products are applied to the surface of the collagen itself, it is difficult to absorb the high percutaneous absorption of collagen, a high molecular material, can not expect a moisturizing effect or wound healing effect, it does not show the essential skin function improvement and wound healing function.

Accordingly, interest in collagen synthesis promoters has increased, and known collagen synthesis promoters include vitamin C, retinoic acid, and TGF (transforming growth factor, Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974).

In addition, Japanese Patent Application Laid-Open No. 8-231370 discloses a protein derived from an animal placenta as a collagen synthesis material, and Japanese Patent Laid-Open No. 8-208424 discloses betulinic acid, Japanese Patent Application Laid-open Nos. 9-40523 and 10. -36283 discloses chlorella extracts and the like that exhibit fibroblast proliferation-promoting action.

However, all of the above-mentioned conventional materials have limitations in use due to safety problems such as irritation and redness when applying skin, or they have a problem that the effect of insignificant effects cannot be expected to substantially improve skin function or wound healing. There is a need for development of a new skin external preparation composition that is safer and more effective in vivo than existing external skin preparation compositions.

The present invention has been made in consideration of the above-described problems of the prior art, and has an excellent stability to the living body and an excellent effect of promoting collagen formation, and provides a composition for promoting collagen formation that can be applied to various formulations due to excellent processability such as solubility in a solvent. It aims to do it.

The present invention as a means for solving the above problems,

Provided is a composition for promoting collagen formation comprising frangenidine and ginsenoside Re.

In the composition of the present invention, the frangenidine is preferably included in the composition in an amount of 1 × 10 ⁻⁵ to 10 parts by weight, and ginsenoside Re is included in the composition in an amount of 1 × 10 ⁻⁵ to 10 parts by weight. desirable.

Moreover, in the composition of this invention, it is preferable that the weight ratio (ginsenoside Re / frangenidine) of ginsenoside Re with franginidin exists in the range of 1-100, and it is more preferable that it exists in the range of 5-30. Do.

The present invention also provides another means for solving the above problems,

It provides a cosmetic for improving wrinkles or inhibiting wrinkles comprising the composition according to the invention.

The formulation of the cosmetic of the present invention is a powder, gel, spray, ointment, cream, liquid, skin adhesive formulation, cream, foam, lotion, pack, soft water, emulsion, foundation, makeup base, essence, soap, Liquid detergents, baths, sunscreen creams, sun oils or spray liquids.

In addition, the cosmetic of the present invention is one selected from the group consisting of antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments and preservatives The above additive may be further included.

The composition of the present invention has an excellent collagen formation promoting ability and collagenase inhibitory ability (collagenase), and especially when a small amount of the active ingredient has the advantage that the effect is exerted. Accordingly, the composition of the present invention is excellent in the ability to suppress and improve the occurrence of wrinkles associated with the reduction of collagen, and also excellent in the healing effect of the wound on the skin. In addition, since the composition of the present invention exhibits excellent efficacy even with a small amount of active ingredients, it can be used in various formulations without limitations such as solubility in solvents, for example, when applied to cosmetics, thereby increasing the scope of application. Has the advantage.

The present invention relates to a composition for promoting collagen formation, including franginidin and ginsenoside Re. The composition of the present invention has an excellent collagen formation promoting ability and collagenase inhibitory ability (collagenase) ability, in particular has the advantage that the effect is exerted excellently even with a small amount of the active ingredient. Accordingly, the composition of the present invention is excellent in the ability to suppress and improve the occurrence of wrinkles associated with the reduction of collagen, it is also excellent in the healing effect of the wound on the skin. In addition, since the composition of the present invention exhibits excellent efficacy even with a small amount of active ingredients, it is excellent in processability when applied to cosmetics and the like, and can be used in various formulations, and thus has an advantage of wider application range.

Hereinafter, the collagen formation promoting composition of the present invention will be described in detail.

The composition for promoting collagen formation of the present invention includes frangenidine and ginsenoside Re. In this case, it is preferable to use a compound represented by the following formula (1) as the frangenidine.

[Formula 1]

Frangenidine represented by Formula 1 has safety in vivo, and has an excellent effect of promoting collagen and hyaluronic acid synthesis (HAS2 mRNA expression enhancing effect) of skin fibroblasts, but solubility in solvents. Apart, its application to cosmetics and the like is very limited. However, in the present invention, by using the ginsenoside Re in combination, it can exhibit excellent collagen formation promoting ability while using a small amount of the above. Accordingly, for example, when the composition of the present invention is used as a cosmetic, it can solve problems such as solubility, exhibit excellent wrinkle improvement and wound healing effect, thereby maintaining the elasticity of the skin.

Frangenidine as described above can be synthesized through various organic and chemical methods known in the art, and can be easily obtained from plants and the like. Frangenidine is present in a lot of lumber and plant roots, in particular, such as copper, and the method of extracting the frangenidine from it is not particularly limited, various extraction methods known in the art can be applied without limitation. For example, chopped roots (white paper) of hawthorn and plants are added to a solvent containing at least one selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone and chloroform. It can be obtained by heating extraction, solvent fractionation and recrystallization.

The composition of the present invention also includes ginsenosides Re, wherein it is preferable to use a compound represented by the following formula (2) as ginsenosides Re.

[Formula 2]

Ginsenoside Re as described above is also excellent in bio stability and collagen synthesis promoting ability. In addition, the ginsenoside Re exhibits the effect of inhibiting the activity of collagen degrading enzyme collagen degrading enzyme, it is included in the present invention to improve the collagen formation promoting ability of the composition. Accordingly, for example, when the composition of the present invention is used as a cosmetic, it serves to enhance skin elasticity by increasing the effect of improving skin wrinkles.

Such ginsenoside Re can be chemically synthesized through various organic chemical methods known in the art, and can be easily obtained from plants and the like, such as frangenidine. The ginsenoside Re is particularly present in ginseng or red ginseng, and the like, and a method of extracting a target compound from the ginsenoside Re is not particularly limited, and various extraction methods known in the art may be applied without limitation. Examples of such a method include chopped ginseng and finely added to a solvent containing water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone and / or chloroform, followed by warm extraction to separate the solvent, followed by a separation and purification process. There is a way to go through.

The composition for promoting collagen formation of the present invention includes frangenidine and ginsenoside Re as described above, and has an advantage of exhibiting excellent collagen formation promoting ability while containing a small amount of the active ingredient. Specifically, in the composition of the present invention, the frangenidine and ginsenoside Re are preferably included in an amount of 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition, and 1 × 10 ^-5 to 5 parts by weight. More preferably included in negative amounts. If the content is less than 1 × 10 ^-5 parts by weight, the collagen formation promoting ability may be lowered. If the content is more than 10 parts by weight, the overall processability of the composition, such as solubility in a solvent, is poor, the use in various applications such as cosmetic formulations described later There is a fear that it will be limited.

In this invention, it is also preferable that the weight ratio (ginsenoside Re / frangenidine) of the said franginidine and ginsenoside Re is 1-100, and it is more preferable that it is 5-30. If the weight ratio is less than 1 or more than 100, the synergistic effect of the collagen synthesis effect may be insignificant.

In the composition for promoting collagen formation of the present invention, additives other than the active ingredient are not particularly limited, and those commonly used in the art may be used without limitation. For example, when the composition is used for medical purposes, a pharmaceutically acceptable conventional carrier may be included, and when applied to cosmetics, conventional additives commonly used in the cosmetic field may be used without limitation.

The present invention also relates to a cosmetic for improving and suppressing wrinkles comprising the composition of the present invention described above. As described above, the composition for promoting the formation of collagen of the present invention has excellent stability to the human body and the ability to form collagen, and is excellent in the suppression and treatment effect of the occurrence of wrinkles, the main cause of which is the reduction of collagen, and thus applied to cosmetics. It can increase skin elasticity, heal wounds and moisturize.

Cosmetics of the present invention as described above can be produced by the production method generally used in the cosmetic field, using the composition for promoting collagen formation of the present invention as an active ingredient, the method is not particularly limited.

When the composition for promoting collagen formation of the present invention is applied to cosmetics, its formulation is not particularly limited. For example, the cosmetic of the present invention may be a cosmetic formulation such as powder, gel, spray, ointment, cream, liquid or skin adhesive type; Creams, foams, lotions, packs, softening fluids, emulsions, foundations, makeup bases, essences, soaps, liquid cleansers, baths, sunscreen creams, sun oils and / or jetted liquids. Additives added in each of the above formulations is not particularly limited, either of the additives commonly used in the cosmetic field may be appropriately added depending on the use thereof. Examples of common additives in the cosmetic field include antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, flavorings, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments and preservatives. One or more selected.

Hereinafter, the present invention will be described in more detail through examples according to the present invention and comparative examples not according to the present invention. However, the scope of the present invention is not limited by the examples presented below.

Production Example  One: Of frangenidin  Produce

(1) using methanol Of frangenidin  extraction

1 kg of dried roots of white paper (Angelica dahurica or Angelica dahurica var. Formosana) were placed in 10 L of methanol and extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 85 g of methanol extract. The fraction obtained by removing the hexane fraction from the methanol extract through a solvent fraction was fractionated three times with chloroform to obtain 9 g of a chloroform fraction. Through several silica column chromatography treatment, 0.3 g of a fraction containing frangenidine was obtained from the chloroform fraction. The fractions were then subjected to preparative HPLC (Prep-HPLC) and recrystallization to afford the desired compound, frangenidine. The components and contents (99.7% by weight) of frangenidine obtained through nuclear magnetic resonance (NMR) and mass spectroscopy were shown in FIGS. 1 to 3, respectively. 1 and 2 show the ¹ H-NMR spectrum and the ¹³ C-NMR spectrum of the obtained frangenidine, respectively, and FIG. 3 shows the mass spectrometry of the frangenidine.

(2) using chloroform Of frangenidin  extraction

1 kg of dried root of white paper was placed in 10 L of chloroform and warm-extracted at 100 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 12 g of chloroform extract. The obtained extract was dissolved in chloroform, solvent fractionated with alkaline aqueous solution (0.1 M NaOH aqueous solution) to obtain an alkaline aqueous solution soluble portion, and neutralized with HCl. Preparative HPLC (Prep-HPLC) and recrystallization were performed on 1 g of the chloroform fraction obtained by solvent fractionation with chloroform to obtain the target compound (frangenidine). Nuclear magnetic resonance (NMR) and mass spectroscopy confirmed the composition and content (99.7% by weight) of the compound obtained.

(3) using methanol Imperatorin ( imperatorin ) And Of cnidirin  Extraction and chemical modification

1 kg of dried root of white paper was placed in 10 L of methanol, and extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 85 g of methanol extract. The extract was fractionated three times with chloroform to obtain 11 g of chloroform fractions. Then, 7 g of a fraction containing impertorin and knidyrin, which are the main components of the white paper, were obtained from the chloroform fraction through silica column chromatography. 25 g of N, N-Diethylanilin was added thereto, and heated at 220 ° C. for 1 hour to carry out Claisen rearrangement. The reactant obtained with a 5N hydrochloric acid solution was washed, dissolved in chloroform and refrigerated to prepare a precipitate. From the precipitate, frangenidine was obtained via preparative HPLC (Prep-HPLC) and recrystallization method. The composition and content (99.7 wt%) of frangenidine obtained through NMR and mass spectroscopy were confirmed.

Production Example  2: Ginsenoside Re Manufacture

5 kg of dry ginseng was ground, and a reflux condenser was used using 10 times the weight of ethanol and heated for 4 hours in a water bath to obtain an ethanol extract. The process was repeated three times and extracted. The extracts were combined, filtered and concentrated under reduced pressure. 2L of hot water at 50 ° C was added to the concentrate to completely dissolve it, followed by extraction with 200 ml of ethyl ether three times to remove fat. Repeated 5 times with 300 ml of saturated n-butanol as a fraction After extracting and obtaining a crude saponin extract, the extract was completely concentrated under reduced pressure to obtain 120 g of crude saponin powder. 6 g of the fraction containing ginsenosides Re was obtained from the powder through silica column chromatography using a C18 resin, and ginsenosides were obtained from the powder using preparative HPLC (Prep-HPLC). It was. The components and contents (99.7 wt%) of ginsenoside Re obtained through nuclear magnetic resonance (NMR) and mass spectroscopy were confirmed. 4 and 5 show the ¹ H-NMR spectrum and mass spectrometry of the ginsenoside Al, respectively.

Test Example  1: Test of Collagen Synthesis Effect

Frangenidine, ginsenoside Re and mixtures thereof prepared in the above preparations were added to the culture solution of human fibroblast cells to test collagen synthesis effects at the cellular level. The measurement of the synthesized collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

In the experiment, the final concentration of franginidine was set to 0.1 µg / ml and 0.5 µg / ml, and the final concentration of ginsenoside Re was 10 µg / ml and 20 µg / ml, respectively. Was as shown in Table 1 below. Each sample was added to the culture medium of human-derived fibroblasts, and cultured for 1 day. The culture solution was taken, and the degree of collagen synthesis at each concentration was measured at 450 nm with a PICP EIA kit using a spectrophotometer. To compare the effects, collagen synthesis was measured by the same method for the fibroblast culture medium (control) and vitamin C to which the final concentration of 52.8 ㎍ / ml was added. Collagen production was measured as UV absorbance, the collagen production increase rate was calculated as the ratio of collagen production relative to the control group, the results are summarized in Table 1.

TABLE 1 Collagen synthesis effects at the cellular level

Sample (μg / ml ) Collagen Generation amount ( Abs ) Increase (%) Franginidin Ginsenoside Re - - 1240 - Vitamin C (52.8 μg / ml) 1566 26.3 0.1 - 1278 3.1 0.5 - 1393 12.3 - 5 1306 5.3 - 10 1411 13.8 0.1 5 1582 27.6 0.1 10 1639 32.2 0.5 5 1605 29.4 0.5 10 1686 36.0

(Number of repetitions = 6)

As can be seen from the results of Table 1, when the frangenidine and ginsenoside Re is mixed, it exhibits better collagen synthesis effect at a lower concentration than when vitamin C, which is generally known to have collagen synthesis ability, is applied. It was. In addition, it was also confirmed that a marked synergistic effect appeared when the above was added at the same time as compared with the case where frangenidine and ginsenoside Re were added alone.

Test Example  2: Ginsenoside Re Inhibitory Effects of Collagen Degrading Enzymes

Ginsenoside Re prepared in Example 2 was added to the culture solution of human-derived fibroblasts at concentrations of 5 μg / ml, 10 μg / ml and 20 μg / ml to inhibit the increase in collagenase expression induced by ultraviolet rays. The effect was tested. The measurement of collagenase was quantified using the BioTrak MMP-1 Assay Kit (Amersham Bioscience), and the obtained results were substituted in Equation 1 to calculate the inhibition rate (%), and the results are shown in Table 2 below. In the following Table 2, the ultraviolet control group is a group irradiated with ultraviolet light only without processing the sample, the negative control group is a group not applied to the sample and ultraviolet light.

Equation 1: Inhibition rate = (ultraviolet control-experimental group) / ultraviolet control X 100

TABLE 2 Collagenase  Expression inhibitory effect

Ginsenoside Re (Μg / ml ) Collagenase  Expression Suppression (A450) % Inhibition 5 0.739 22 10 0.540 43 20 0.493 48 Negative Control 0.537 - UV control 0.948 -

(Number of repetitions = 3)

As shown in Table 2, from the test results, it can be seen that ginsenoside Re exhibits an excellent inhibitory effect of collagenase expression.

Test Example  3: skin wrinkle improvement effect test

Using a 6-week-old mouse, the skin wrinkle improvement effect of frangenidine, ginsenoside Re and the mixed sample was tested for skin wrinkles caused by light. Frangenidine and ginsenoside Re prepared in the above-mentioned preparation was dissolved in 1,3-butylene glycol at a concentration of 5 mg / ml, respectively, and the mixed samples were found to have concentrations of franginidin and ginsenoside Re. Adjustment was applied to 0.5 mg / ml and 4.5 mg / ml, respectively. Specifically, the mouse was irradiated with 3 MED 3 days per week for 10 weeks using a solar simulator (solar simulator) to form skin wrinkles, a control group treated with 1,3-butylene glycol without addition of the sample And 5 mg / ml sample solution twice a day 0.5 ml / ㎠ (2.5 mg / ㎠ as a sample) was treated for 6 weeks to determine the degree of improvement qualitatively. Wrinkle improvement was judged visually by photographing and photographing the sample processing area, and the judgment criteria were judged as three stages of no improvement, slight improvement, and significant improvement compared to the sample treatment group and control group before the sample treatment. It is shown in Table 2 below.

[Table 3] Effect of improving skin wrinkles on mice

No improvement Minor improvements A significant improvement Control 9 One 0 Franginidin 3 4 3 Ginsenoside Re 2 5 3 Mixed sample 0 6 4

When Frangenidine and ginsenosides Re is mixed from Table 2, it was confirmed that a significant effect increase was observed compared to the case where each of them was used alone.

Example  1 and Comparative example  1 to 3

Using a general method in the field of cosmetic manufacturing has the composition shown in Table 3, the remaining amount was purified water to prepare a cosmetic essence adjusted to 100 parts by weight of the total weight.

TABLE 4

Example  One Comparative example  One Comparative example  2 Comparative example  3 Franginidin 0.05 - 0.5 - Ginsenoside Re 0.45 - - 0.5 Propylene glycol 10.0 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 10.0 Sodium hyaluronate ( Sodium Hyaluronic acid , One%) 5.0 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene  Cured Castor Oil 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 0.1 incense 0.05 0.05 0.05 0.05

(Unit: parts by weight)

Test Example  4: Cosmetics Essence  Wrinkle improvement effect test

The skin wrinkle improvement effect of the cosmetic essences of Example 1 and Comparative Examples 1 to 3 was tested through a panel test. Specifically, 60 healthy women from 35 to 50 years old were divided into four groups of 15 people, group 1 of the essence of Example 1, group 2 of the essence of Comparative Example 1, group 3 of the comparative example 2 Essence was applied to the group 4, the essence of Comparative Example 3 was applied to the face part once a day for 3 months, and the degree of improvement of skin wrinkles was evaluated through a survey of the subject. The subject's questionnaire was judged in three stages of improvement of skin wrinkles and elasticity compared with before use, no improvement, slight improvement, and significant improvement. The results are shown in Table 5.

TABLE 5 Example  Skin wrinkle improvement effect (survey)

No improvement Minor improvements A significant improvement Comparative example  One 9 4 2 Comparative example  2 2 9 4 Comparative example  3 4 10 One Example  One One 7 7

As can be seen from Table 5, in the case of Example 1 using the frangenidine and ginsenoside Re, the skin wrinkle improvement effect than the essence of Comparative Examples 1 to 3 using each of these alone or not used Was confirmed to be excellent.

Test Example  5: through image analysis Cosmetics Essence  Wrinkle improvement effect test

The wrinkle improvement effect of Example 1 and Comparative Examples 1-3 was evaluated through image analysis. Specifically, replicas under the eyes (Xantopren, Bayer) are taken before the experiment begins, and replicas are taken from the same site immediately after the experiment is completed. The density of the wrinkles was measured. The reduction rate of skin wrinkle density by image analysis is a value obtained by averaging the ratio of skin wrinkle density after use to skin wrinkle density before using essence, and the measurement results are summarized in Table 6 below.

[Table 6] Improvement of skin wrinkles by image analysis

Skin wrinkle density reduction rate (%) Comparative example  One 4 Comparative example  2 35 Comparative example  3 21 Example  One 42

As can be seen from Table 6, in the case of Example 1 using Frangenidine and Ginsenoside Re, the wrinkle improvement effect superior to the essence of Comparative Examples 1 to 3 using each of these alone or not used It was confirmed that there is. In addition, no skin irritation or side effects due to the essence of Example 1 was found in the test procedure.

Example  2

Through each of the compositions shown below, various cosmetic formulations were prepared.

Skin ointment  (unit: Parts by weight )

Frangenidine: 0.1

Ginsenoside Re: 0.9

Diethyl Sebacate: 8

Prepayment: 5

Oleyl ether phosphate: 6

Sodium benzoate: 0.1

Adjust the total weight to 100 by using Waselin

Cosmetics  cream  (unit: Parts by weight )

Frangenidine: 0.02

Ginsenoside Re: 0.18

Stearic Acid: 15.0

Cetanol: 1.0

Potassium Hydroxide: 0.7

Glycerin: 5.0

Propylene Glycol: 3.0

Preservative: 0.05

Incense: 0.05

Adjust the total weight to 100 by the amount of purified water remaining

Flexible lotion  (unit: Parts by weight )

Frangenidine: 0.02

Ginsenoside Re: 0.18

Ethanol: 10.0

Polylauric acid polyoxyethylene sorbitan: 1.0

Methyl Paraoxybenzoate: 0.2

Glycerin: 5.0

1,3-butylene glycol: 6.0

Incense: 0.05

Pigment: 0.05

Adjust the total weight to 100 by the amount of purified water remaining

Nutrition lotion  (unit: Parts by weight )

Frangenidine: 0.01

Ginsenoside Re: 0.09

Waselin: 2.0

Sesquioleic acid sorbitan: 0.8

Polyoxyethylene oleylethyl: 1.2

Methyl paraoxybenzoate: 0.1

Propylene Glycol: 5.0

Ethanol: 3.2

Carboxy Vinyl Polymer: 18.0

Potassium Hydroxide: 0.1

Pigment: 0.05

Incense: 0.05

Adjust the total weight to 100 by the amount of purified water remaining

Cosmetics  pack  (unit: Parts by weight )

Frangenidine: 0.02

Ginsenoside Re: 0.18

Glycerin: 5.0

Propylene Glycol: 4.0

Polyvinyl Alcohol: 15.0

Ethanol: 8.0

Polyoxyethylene oleylethyl: 1.0

Methyl Paraoxybenzoate: 0.2

Pigment: 0.05

Incense: 0.05

Adjust the total weight to 100 by the amount of purified water remaining

For men skin  (unit: Parts by weight )

Frangenidine: 0.01

Ginsenoside Re: 0.09

Ethanol: 55.0

Castor oil (PEG-40 Hydrogenated castor oil): 0.5

1,3-butylene glycol: 1.0

Glycereth-26: 1.0

Preservative: 0.05

Incense: 0.05

Adjust the total weight to 100 by the amount of purified water remaining

As described above, the composition for promoting collagen synthesis containing frangenidine and ginsenoside Re as an active ingredient exhibits a very strong collagen synthesis effect on fibroblasts of the skin, and the composition has excellent antiwrinkle effect. . When the composition of the present invention is applied to cosmetic formulations, while using a low concentration of frangidine, which is difficult to be applied to general cosmetic formulations, while obtaining a sufficient wrinkle improvement effect, it can be applied to products of various formulations. It can be applicable. The synergistic effect of the combined application of Frangenidine and Ginsenoside Re is synergistic effect with each other by appropriately mixing Frangenidine and Ginsenoside Re, which is excellent in collagen biosynthesis performance and collagenase expression suppression effect. It seems to be.

1 is a diagram showing a ¹ H-NMR spectrum of the frangenidine prepared in Example 1.

FIG. 2 is a diagram showing ¹³ C-NMR spectra of frangenidine prepared in Example 1. FIG.

3 is a diagram showing a mass spectrum of frangenidine prepared in Example 1. FIG.

4 is a diagram showing a ¹ H-NMR spectrum of ginsenoside Re prepared in Example 2. FIG.

5 is a diagram showing a mass spectrum of ginsenoside Re prepared in Example 2. FIG.

Claims (10)

Composition for promoting collagen formation comprising frangenidine and ginsenoside Re. The method of claim 1, Frangenidine is a composition for promoting collagen formation, characterized in that contained in an amount of 1 × 10 ^-5 to 10 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 2, Frangenidine is a composition for promoting collagen formation, characterized in that contained in an amount of 1 × 10 ^-5 to 5 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 1, Ginsenoside Re is a composition for promoting collagen formation, characterized in that contained in an amount of 1 × 10 ^-5 to 10 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 4, wherein Ginsenoside Re is a composition for promoting collagen formation, characterized in that contained in an amount of 1 × 10 ^-5 to 5 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 1, A composition for promoting collagen formation, characterized in that the weight ratio (ginsenoside Re / frangenidine) of ginsenosides Re to frangenidine is 1 to 100. The method of claim 6, A composition for promoting collagen formation, characterized in that the weight ratio (ginsenoside Re / frangenidine) of ginsenosides Re to franginidin is 5 to 30. A cosmetic for improving wrinkles or inhibiting wrinkles, comprising the composition of any one of claims 1 to 7. The method of claim 8, Formulations are powders, gels, sprays, ointments, creams, liquids, skin adhesive formulations, creams, foams, lotions, packs, softeners, emulsions, foundations, makeup bases, essences, soaps, liquid cleansers, baths, sunscreens Cosmetics, characterized in that the cream, oil or spray liquid. The method of claim 8, Cosmetics add one or more additives selected from the group consisting of antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments and preservatives. Cosmetics characterized in that it comprises as.

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