KR20070119827A - Clean Growth Act of Korean Ginseng Seeds - Google Patents
Clean Growth Act of Korean Ginseng Seeds Download PDFInfo
- Publication number
- KR20070119827A KR20070119827A KR1020060054250A KR20060054250A KR20070119827A KR 20070119827 A KR20070119827 A KR 20070119827A KR 1020060054250 A KR1020060054250 A KR 1020060054250A KR 20060054250 A KR20060054250 A KR 20060054250A KR 20070119827 A KR20070119827 A KR 20070119827A
- Authority
- KR
- South Korea
- Prior art keywords
- korean ginseng
- germination
- ginseng seed
- ginseng seeds
- korean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 28
- 240000004371 Panax ginseng Species 0.000 title claims abstract description 26
- 235000002789 Panax ginseng Nutrition 0.000 title claims abstract description 26
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000645 desinfectant Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000004480 active ingredient Substances 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 239000003960 organic solvent Substances 0.000 abstract description 3
- 231100000344 non-irritating Toxicity 0.000 abstract description 2
- 230000009965 odorless effect Effects 0.000 abstract description 2
- 230000035784 germination Effects 0.000 description 14
- 238000011282 treatment Methods 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 206010020649 Hyperkeratosis Diseases 0.000 description 8
- 239000000203 mixture Substances 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 241000208340 Araliaceae Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012669 liquid formulation Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000766754 Agra Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- WQLUOXFADPZIRD-UHFFFAOYSA-N s-(dimethylcarbamoylsulfanyl) n,n-dimethylcarbamothioate;methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate Chemical compound CN(C)C(=O)SSC(=O)N(C)C.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WQLUOXFADPZIRD-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S47/00—Plant husbandry
- Y10S47/01—Methods of plant-breeding and including chromosome multiplication
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S47/00—Plant husbandry
- Y10S47/09—Physical and chemical treatment of seeds for planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
본 발명은 무색, 무취, 무자극성이며 물과 유기용매에 용해도가 크며 열안정성이 큰 4급 염화암모늄을 유효성분으로 함유하는 고려인삼종자의 소독제제와 이를 이용한 고려인삼종자 유식물의 청정육성법에 관한 것이다. The present invention is a colorless, odorless, non-irritating, disinfectant of Korean ginseng seed containing quaternary ammonium chloride having high solubility in water and organic solvents and high thermal stability as an active ingredient, and the method for clean growth of Korean ginseng seedlings using the same. It is about.
Description
본 발명의 고려인삼종자의 청정육성법에 관한 것이다. It relates to a clean growth method of Korean ginseng seed of the present invention.
더욱 상세하게는, 본 발명은 4급 염화암모늄화합물인 알킬디메틸벤질염화암모늄과 알킬디메틸에틸벤질염화암모늄의 단독 또는 혼합물을 주제로 한 고려인삼종자 소독제 및 이를 이용한 고려인삼종자의 청정발아법에 관한 것이다. More specifically, the present invention relates to a disinfectant of Korean ginseng seed based on the sole or mixture of alkyldimethylbenzyl ammonium chloride and alkyldimethylethylbenzyl ammonium quaternary ammonium chloride compound and clean germination method of Korean ginseng seed using the same. .
지금까지 고려인삼종자의 인공발아법의 연구는 전무한 실정이며 본 발명자들의 연구결과에 따르면, 인공 발아 후 주위환경에 따라 발아물이 용이하여 부패하여 폐기되기 쉽고 1㎝ 이상 길이가 되도록 성장시 발아물의 선도유지가 용이하지 않았다. So far, there are no studies on artificial germination of Korean ginseng seeds, and according to the results of the present inventors, the germination of fresh germination when grown to grow to be more than 1 cm long due to easy germination according to the surrounding environment. It was not easy to maintain.
본 발명자들은 고려인삼종자의 연중발아법에 관하여 이미 10-581729 호로 특허등록한 바 있으며, 캘러스와 엽아 혼용물 인공배양에 관하여도 국내특허 제 307503 호로 개시한 바 있다.The present inventors have already registered a patent for the annual germination method of Korean ginseng seed as 10-581729, and also disclosed in Korean Patent No. 307503 for artificial culture of callus and leaf germination.
본 발명자들은 이와 같이 종래의 연구결과들을 통하여 예의 연구한 결과 4급 염화암모늄화합물의 처리가 고려인삼종자의 고온 및 저온 과습 및 건조상태의 광차 단된 밀실배양 또는 발아처리과정에서 어렵게 캘러스 또는 발아된 유식물이 곰팡이 등 진균이나 병원성 세균에 의해 부패 되는 것을 방지할 필요가 있었음을 절감한 바 있어 이에 공표하는 것이다.Thus, the present inventors have diligently studied the results of the conventional research results, and the treatment of quaternary ammonium chloride compound is difficult to callus or germinate oil during photo-blocked closed room culture or germination treatment of Korean ginseng seeds at high and low temperature and high humidity and dry conditions. It was announced that it was necessary to prevent the plants from being decayed by fungi such as fungi or pathogenic bacteria.
종래에도 종자소독제로 에틸알콜이 사용되었으나 발아과정에서 병원성 세균의 감염증식을 억제하기 곤란함이 확인되었다.In the past, ethyl alcohol was used as a seed disinfectant, but it was confirmed that it is difficult to suppress infection of pathogenic bacteria during germination.
또 본 발명자들은 일반적으로 두과식물 종자의 부패를 유발하는 원인균의 멸균을 위해 흔히 사용되는 지오람수화제(아벤티스사, 호마이 제품), 카보람분제(동방아그라사, 비타지람 제품), 만코지수화제(경농, 다이센엠 45 제품) 등을 사용하여 보았으나 이들은 살균효과는 우수하나 고려인삼종자의 캘러스나 엽아를 식용이나 약용으로 사용하고자 하는 본 발명에서는 이들의 잔류 독성으로 인하여 적합하지 않았음을 확인하였다. In addition, the inventors of the present invention are commonly used for sterilization of the causative bacteria causing decay of legumes, such as georamum (Aventis, Homai), carboram powder (East Agra, Vitajiram), mankoji (Gyeongnong, Daisen M 45 product) and the like, but they have excellent sterilization effect, but in the present invention to use the callus or leaf buds of Korean ginseng seed for food or medicinal use was confirmed that it was not suitable due to their residual toxicity .
또, 고려인삼종자 캘러스 또는 발아물을 식용이나 약용으로 하는 경우에는 부패원인균에 대한 살균력이 확실하고 저독성이면서 인체에 무해하며 잔류 독성이 적어 안전성에 문제가 없으며 처리법이 간편하고 가격이 저렴한 것이 요구되며 또 고려인삼종자의 캘러스 또는 발아유도에는 저온과 고온조건 저습과 고습 조건의 반복처리가 필요하고 압 조건과 환기가 어려운 무료 밀폐공간이 사용되어야 한다는 특성이 있으므로 이들 조건을 모두 만족할만한 화학물질이 요구되어 왔다. In addition, when Korean ginseng seed callus or germination is used for food or medicinal purposes, sterilizing power against rot-causing organisms is sure, low toxicity, harmless to human body, low residual toxicity, no safety problems, simple treatment and low price. In addition, callus or germination induction of Korean ginseng seeds requires low-temperature, high-temperature, low-humidity and high-humidity repetitive treatments, and free enclosed spaces that are difficult to pressurize and ventilate. Has been.
본 발명의 상기 목적은 무색, 무취, 무자극성이며 물과 유기용매에 용해도가 크며 열안정성이 큰 4급 염화암모늄 제제를 유효성분으로 하는 수용액 또는 유기용 매 용액에 고려인삼종자를 9시간 ~ 12시간 침지한 다음 무균실에 옮겨 발아시킴으로써 달성하였다. The object of the present invention is colorless, odorless, non-irritating, solubility in water and organic solvents, and high thermal stability quaternary ammonium chloride formulation as an active ingredient in aqueous solution or organic solvent solution for 9 hours to 12 Achieved by germination in time and transfer to a clean room to germinate.
이하, 본 발명의 구체적인 방법을 구체적인 실시예를 통하여 설명하지만 이에 유사한 화합물을 사용하거나 발아처리상 단순한 수치변경은 본 발명의 권리범위에 속하는 것은 물론이다. Hereinafter, the specific method of the present invention will be described with reference to specific examples, but using a compound similar thereto or simply changing the numerical value in the germination process is of course within the scope of the present invention.
<실시예 1> 고려인삼종자 살균소독제제의 제조Example 1 Preparation of Korean Ginseng Seed Sterilization Agent
n-알킬디메틸벤질염화암모늄과 n-알킬디메틸에틸벤질염화암모늄을 중량비 1:1로 혼합한 후, 에틸알콜과 정제수 중량비 1:1로 혼합한 용액 1L에 투입하여 1시간 균일하게 혼합한 액상 살균제 스톡(Stock)을 제조하였다. n-alkyldimethylbenzyl ammonium chloride and n-alkyldimethylethylbenzyl ammonium chloride were mixed in a weight ratio of 1: 1, and then added to 1 L of a mixture of ethyl alcohol and purified water in a weight ratio of 1: 1. Stock was prepared.
크린벤치상에서 상기 액상제제 100mL를 10L의 물에 희석하여(100×액) 여기에 고려인삼종자 10㎏을 10시간 침지하여 액상제제가 종실에 잘 침투되도록 한 다음 무균육성실로 이송하였다. On the clean bench, 100 mL of the liquid formulation was diluted in 10 L of water (100 × liquid), and 10 kg of Korean ginseng seed was immersed for 10 hours to allow the liquid formulation to penetrate the seed well, and then transferred to a sterile growth chamber.
<실시예 2> 고려인삼종자 유식물의 무균육성Example 2 Aseptic Growth of Korean Ginseng Seedlings
공지된 무균육성실내의 온도와 습도는 자동조절기에 의하여 제어(control)되도록 하였다. The temperature and humidity in the known aseptic growing room were controlled by an automatic regulator.
상기 실시예 1에 따라 제조된 살균소독제제로 소독한 고려인삼종자를 무균적으로 육성하기 위하여 크린벤치에서 자동적으로 무균육성실로 콘베이어벨트로 이송하였다. In order to aseptically cultivate the Korean ginseng seed sterilized with the disinfectant disinfectant prepared according to Example 1, it was automatically transferred from the clean bench to the aseptic growth chamber to the conveyor belt.
무균육성실로 이동된 고려인삼종자에 대하여 상대습도 60% 조건으로 -5℃에 서 5시간 저온처리시킨 다음, 다시 상대습도 90% 조건으로 20℃로 3시간 고온처리하였다. The Korean ginseng seed that was transferred to the sterile growing room was cold-treated at -5 ° C for 5 hours at 60% relative humidity, and then heated at 20 ° C for 3 hours at 90% relative humidity.
고온처리된 고려인삼종자를 -5℃로 다시 조절하여 상기 동일한 60% 상대습도 조건하에서 5시간 저온처리한 다음 다시 상대습도 90% 조건의 20℃에서 3시간 고온처리하는 방법으로 1일 3회 반복처리(repeat treatment) 하기를 3주간 계속한 결과 엽아길이 0.9~3.0㎝의 고려인삼종자 발아유식물을 얻을 수 있었다. The Korean ginseng seed treated with high temperature was re-adjusted at -5 ° C for 5 hours under the same 60% relative humidity condition and then subjected to high temperature treatment at 20 ° C under 90% relative humidity for 3 hours. Repeat treatment was continued for three weeks, and germinated seedlings of Korean ginseng seed with leaf length of 0.9 ~ 3.0㎝ were obtained.
<실시예 3> 고려인삼종자로부터 무균 캘러스-엽아 혼용물 세포배양Example 3 Culture of Sterile Callus-Leave Combination Cells from Korean Ginseng Seeds
실시예 1에서 얻은 살균제제로 무균처리한 인삼종자를 실시예 2의 방법으로 얻은 발아유식물로부터 1~2㎜ 크기의 자엽을 절취하여 크린벤치상에서 아가(agar) 5 ~ 10g/L, IAA 1 ~ 3㎎/L, 키네틴(kinetin) 5~15㎎/L, 수크로스(Sucrose) 20 ~ 40g/L를 함유한 pH 5.5 ~ 6.0, 바의 MS 고체배지에 치상하고 6개월 후에 캘러스와 엽아가 혼화된 신초단계의 무료 유식물을 취득하였다. Ginseng seeds treated aseptically with the fungicides obtained in Example 1 were cut from cotyledons of 1-2 mm size from the germination seedlings obtained by the method of Example 2, and agar 5 to 10 g / L, IAA 1 to 1 on a clean bench. Callus and leaf buds were mixed after 6 months of treatment on MS medium of pH 5.5-6.0, bar containing 3mg / L, kinetin 5-15mg / L, sucrose 20-40g / L Free seedlings were obtained.
상기 실시예 1의 배지 2를 통하여 얻은 발아유식물 또는 신초단계의 유식물의 종래 무처리(소독실시 아니함) 또는 에틸알콜 단독처리(통제구)에 비하여 실험군을 대조구로하여 육성 또는 배양실험을 난괴법(randomised block design)으로 3회 반복하여 얻은 결과를 고려인삼종자 100립에 대한 이병율 및 약해유무의 달관검사(field test)를 조사한 비교자료는 표 1과 같다. Compared to the conventional untreated (not sterilized) or ethyl alcohol alone (control) of germinated seedlings or shoot seedlings obtained through the medium 2 of Example 1, the experimental group was grown as a control group, or the egg mass was cultured. Table 1 shows a comparison of the field test for the morbidity and weakness of 100 Korean ginseng seeds based on the results obtained by the repeated block design three times.
[표 1]TABLE 1
이상 실시예와 실험예를 통하여 알 수 있는 바와 같이 본 발명의 4급 염화암모늄화합물의 혼합물을 유효성분으로 하는 고려인삼종자 살균소독제는 고려인삼종자의 살균소독효과가 크고 발아유식물이나 신초유기단계의 캘러스-엽아 혼합물의 갈변현상이 없는 양호한 무균육성과 세포배양이 가능한 효과가 있다. As can be seen from the above examples and experimental examples, the Korean ginseng seed disinfectant disinfectant comprising the mixture of the quaternary ammonium chloride compound of the present invention as an active ingredient has a high germicidal disinfection effect of Korean ginseng seed and germinated seedlings or shoots. It is possible to have good aseptic growth and cell culture without browning of the callus-lobe mixture of.
따라서, 희귀한 인삼종자의 유용성을 제고하며 무균 고려인삼종자 유식물을 제공함으로서 건강 음료 및 의약품 산업상 매우 유용한 발명인 것이다. Therefore, it is a very useful invention for the health beverage and pharmaceutical industry by improving the usefulness of rare ginseng seeds and providing sterile Korean ginseng seedlings.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020060054250A KR101095966B1 (en) | 2006-06-16 | 2006-06-16 | Clean Growth Act of Korean Ginseng Seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020060054250A KR101095966B1 (en) | 2006-06-16 | 2006-06-16 | Clean Growth Act of Korean Ginseng Seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20070119827A true KR20070119827A (en) | 2007-12-21 |
| KR101095966B1 KR101095966B1 (en) | 2011-12-20 |
Family
ID=39137899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020060054250A Expired - Fee Related KR101095966B1 (en) | 2006-06-16 | 2006-06-16 | Clean Growth Act of Korean Ginseng Seeds |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101095966B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101032457B1 (en) * | 2009-03-23 | 2011-05-03 | 이종천 | Ginseng Seedling Method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202126B (en) * | 2012-01-12 | 2015-04-01 | 东港天参源参业有限公司 | Ginseng seedling culture and flatland ginseng planting method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4078087A (en) | 1976-03-19 | 1978-03-07 | Hyman Sam M | Method and composition for treating trees using certain quaternary ammonium compounds |
| KR100581729B1 (en) * | 2002-12-30 | 2006-05-22 | 최용조 | Annual Germination of Korean Ginseng Seeds |
-
2006
- 2006-06-16 KR KR1020060054250A patent/KR101095966B1/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101032457B1 (en) * | 2009-03-23 | 2011-05-03 | 이종천 | Ginseng Seedling Method |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101095966B1 (en) | 2011-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mahmoud et al. | Effect of different sterilization methods on contamination and viability of nodal segments of Cestrum nocturnum L | |
| KR102629108B1 (en) | Planting Medium Antimicrobial Composition | |
| JPH05148115A (en) | Simple process for sowing, sprouting and transplantation of orchid | |
| CN108375658B (en) | Method for evaluating control effect of bactericide on black spot of potato seedlings | |
| CN112741087A (en) | Cold chain food disinfectant and preparation method thereof | |
| EP1623623A1 (en) | Antibacterial antiviral composition | |
| Al-Jubori et al. | Optimizing media sterilization via chlorine dioxide and autoclaving of paulowni micropropagation | |
| KR101095966B1 (en) | Clean Growth Act of Korean Ginseng Seeds | |
| CN110800754B (en) | A compound preparation for preventing and treating peach bacterial perforation and its application | |
| JP2016000005A (en) | Germicidal treatment method at the budding time of rice seeds | |
| Blom et al. | Preplant copper-based compounds reduce Erwinia soft rot on calla lilies | |
| CN117426380A (en) | Plant bactericide composition for preventing and treating black rot of broccoli | |
| CN116508792A (en) | Composite disinfectant | |
| KR102760371B1 (en) | Planting Medium Antimicrobial Composition | |
| KR0139442B1 (en) | Method for cultivating bean sprouts and green bean sprouts | |
| CN108606978A (en) | A kind of milk cow hoof liquid formulation and preparation method thereof | |
| CN110731337B (en) | Composition for preventing and treating plant diseases and application thereof | |
| CN112674103A (en) | Antibacterial composition, pesticide bactericide and method for preventing and treating diseases of crops | |
| KR950012090B1 (en) | Additives for sterilization in plant tissue culture | |
| CN110800750A (en) | Pesticide bactericidal composition and preparation thereof | |
| JPS62277305A (en) | Controlling agent against plant pathogenic germ | |
| CN105660694B (en) | A kind of Acidovorax Avenae Subsp. Citrulli seed disinfectant and its application | |
| RU2826724C1 (en) | Method of producing aseptic cultures of plant explants in vitro | |
| CN116267966B (en) | Application of hematoporphyrin monomethyl ether and growth regulator containing hematoporphyrin monomethyl ether | |
| CN113383797B (en) | Tetramycin-containing bactericidal composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20060616 |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20080820 Comment text: Request for Examination of Application Patent event code: PA02011R01I Patent event date: 20060616 Comment text: Patent Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20100915 Patent event code: PE09021S01D |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20110728 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20111130 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20111213 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20111214 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| FPAY | Annual fee payment |
Payment date: 20141210 Year of fee payment: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20141210 Start annual number: 4 End annual number: 4 |
|
| PC1903 | Unpaid annual fee |
Termination category: Default of registration fee Termination date: 20160711 |
|
| FPAY | Annual fee payment |
Payment date: 20160711 Year of fee payment: 5 |
|
| PR0401 | Registration of restoration |
Patent event code: PR04011E01D Patent event date: 20160711 Comment text: Registration of Restoration |
|
| PR1001 | Payment of annual fee |
Payment date: 20160711 Start annual number: 5 End annual number: 5 |
|
| R401 | Registration of restoration | ||
| FPAY | Annual fee payment |
Payment date: 20161212 Year of fee payment: 6 |
|
| PR1001 | Payment of annual fee |
Payment date: 20161212 Start annual number: 6 End annual number: 6 |
|
| FPAY | Annual fee payment |
Payment date: 20171102 Year of fee payment: 7 |
|
| PR1001 | Payment of annual fee |
Payment date: 20171102 Start annual number: 7 End annual number: 7 |
|
| FPAY | Annual fee payment |
Payment date: 20181210 Year of fee payment: 8 |
|
| PR1001 | Payment of annual fee |
Payment date: 20181210 Start annual number: 8 End annual number: 8 |
|
| FPAY | Annual fee payment |
Payment date: 20191128 Year of fee payment: 9 |
|
| PR1001 | Payment of annual fee |
Payment date: 20191128 Start annual number: 9 End annual number: 9 |
|
| PR1001 | Payment of annual fee |
Payment date: 20201210 Start annual number: 10 End annual number: 10 |
|
| PR1001 | Payment of annual fee |
Payment date: 20211212 Start annual number: 11 End annual number: 11 |
|
| PR1001 | Payment of annual fee |
Payment date: 20221203 Start annual number: 12 End annual number: 12 |
|
| PC1903 | Unpaid annual fee |
Termination category: Default of registration fee Termination date: 20240924 |