KR20070075274A - Continuous processing step for functional material of brown algae and products using the same. - Google Patents

Abstract

The present invention is concentrated and low molecular weight by continuous extraction step by step according to its characteristics from the brown algae growing or growing on the coast of Korea according to its characteristics, and dried and pulverized as necessary according to the particle size Acquire quasi-nanoparticles (powder). Research and development of raw materials for various foods (health foods, nutritional supplements or favorite foods) and medicines. Depending on the combined state, several natural substances can be separated and extracted step by step in succession, to obtain individual substances, and to be absorbed into low molecular weights (with oligomers), or dried and ground to make semi-nano particles (powders). It can be digested and absorbed and supplied as a raw material for processing healthy foods while reducing waste and preventing pollution. Extraction, concentration, low molecular weight, drying, and semi-nano particle (powder) processing and acquisition to obtain environmentally friendly and highly effective bioactive substances, such as applying continuous extraction and processing (processing) processes. It's about.

Description

Continuous processing methods for brown seaweeds and their products. {Continuous processing methods for the functional materials from brown seaweed}

Until now, brown algae have been harvested from the sea, dried and crushed, extracted with hot water or acid or alkaline aqueous solution hydrothermal treatment, and one residue is produced to dispose of the environment. Undried) brown algae (seaweed, seaweed, kelp, rice and rhubarb) or dry brown algae, irrespective of their condition, washed them with drinking water and then cut into about 10-15 centimeters of extraction tank (500 liters or 5 tons depending on quantity) Put 2 times the volume of tap water (10-20 times as dry matter), stir and extract it for about half an hour (preferably 10-90 minutes), filter it, transfer it to a concentrator (Brix: 5-10), and sterilize it. Prepare kelp-liquid (1-1 obtained) or dry it with a dryer (frozen, sprayed or furate) to prepare seasoning salts ( 1-2 obtained ) and as many times as desired (preferably Preferably 2-3 times) to prepare special seasonings for collecting semi-nanoparticles (1-3 obtained) .

The residue is transferred to a large grinder (100 liters or 300 liters), and the alcohol (95%) is added to the brown algae so that it is finely cut (approximately 1 square centimeter) and re-transmitted to the extraction tank. The alcohol (adjusted to alcohol concentration 75-90%) was adjusted to 1-2 times the capacity of brown algae, stirred and extracted at room temperature for 18-24 hours, filtered, transferred to a concentrator and concentrated under reduced pressure (-700mmHg) to completely remove the alcohol ( Recovery and recycling) Continued concentration and drying with a freeze dryer, followed by distillation 2-3 times with alcohol (95%) to separate chlorophyll (2-1 obtained) and brown algae [ Fucoxanthin ( 2-2 obtained )] (Extract) and extract the spirits from the residue manitol ( 2-3 obtained ) and use it as a raw material for dry or mannitol-containing products.

5-10mM calcium chloride aqueous solution was added to the residue of the extraction tank, and the residue was heated with stirring. The mixture was stirred and heated at 70-90 degrees Celsius overnight (18-24 hours), filtered and concentrated by a concentrator (about 4-5 times). Secured as a raw material of crude fucoidan (3-1 obtained ), convert degrading enzymes (derived from abalone) or diluted vinegar into applied oligomers, and use them to prepare health drinks (add vitamins and oligosaccharides, etc.) It is supplied as a lyophilized health food ingredient. In addition, column chromatography is performed on crude fucoidan as needed to obtain and separate laminarin and fucoidan.

Into the residue of the extraction tank, 20-25 mM potassium carbonate aqueous solution was added twice the capacity of the residue, and stirred for 2-4 hours while heating (70-80 degrees Celsius) to obtain alginic acid (4-1 obtained ). Concentrate under reduced pressure (-700mmHg) (about 5 times), neutralize with organic acid (vinegar or lactic acid), add more enzyme (derived abalone) or vinegar (0.6% vinegar concentration) and decompose for 3 hours at 95-100 degrees Celsius. After making oligomers, add vitamins and oligosaccharides, etc. Kelp-fiber beverages or precipitated with 2-3 times of alcohol (95%) and freeze or spray-dried for use as a separate alginic acid oligomer (preferably 2- 3) Grinding Semi-nano particles are obtained and supplied as health food ingredients.

Take out the residue of the remaining extraction tank and mix well with dried kelp powder to adjust the moisture content to 35-45% and mix well with vitamin C etc. to obtain kelp granules (obtained 5-1) or kelp pill (acquired 5-2 ) Water) or mix kelp powder with flour 5-10% and pass the residue and dough granulator first, then use a dry noodles to make kelp-noodles (obtained 5-3) or kelp-enriched fish (obtained 5-4) Water) to reduce waste and prevent contamination.

In addition, since alginic acid and fucoidan are indigestible polymers of the human body, they are concentrated and digested after extraction at each step, and then decomposed at room temperature using degrading enzymes (enzymes obtained from live abalone: Fucoidanase and Alginate lyase). It includes the use of dilute vinegar (0.6%) at high temperature (90-100 degrees Celsius) to decompose beverages.

In addition, the crude fucoidan extract is also extracted with laminarin (Polystyrene) Amberlite XAD-2 or Cavalink column, such as purer fucoidan (3-1 obtained) and laminarin (3-2 obtained) It also includes acquiring ) .

It also includes the process of preparing and collecting quasi-nanoparticles (powder) by drying and crushing it several times (preferably 2-3 times) in order to preserve the obtained oligomer for longer periods of time and to absorb it well.

Extract salt from brown algae and concentrate to prepare natural flavorings such as essences, seasoning salts or special salts, and continuously extract functional ingredients from the residue step by step to concentrate, low molecular weight, dry and grind semi-nanoparticles ( Powder) and feed it as a health food raw material. First, we develop a process to continuously extract, concentrate and separate the bioactive substances of brown algae step by step according to their function, and make the polymer material low molecular weight to make oligomer or semi-nano. Development of processes to increase the efficiency of absorption by making particles (powder) and to utilize all of them without throwing them away and to produce environmentally friendly products to prevent environmental pollution.

In the registered or published patent literature or in the actual production plant, it is shown that the extract is used for one of the importance of brown algae, and the residues are discarded.In addition, the salty taste of brown algae is twice as high as that of sodium chloride, unlike ordinary salts. It can be an excellent seasoning to prevent adult diseases, but the process of separating and using it is not applied, and desalination can remove salts such as sodium from other extracts.

The Korean Patent Office Publication No. 0178449 method is very salty in extracting and condensing salts without extracting and removing salts from brown algae, especially kelp. There is,

In the extraction of fucoidan from brown algae, especially kelp, 25-45 times (V / W) of water is added to the raw material powder, and the mixture is concentrated by stirring and extracting for 0.5-2 hours with hot water of 60-80 degrees Celsius. After separation, fucoidan is precipitated with alcohol (75-95%) to obtain centrifugation, and the pH is adjusted to 3.9-6.9 with organic acid, and the pH of 7.1-10.1 after extraction is difficult to extract. The amount of water (water) used in the process and the consumption of alcohol for the precipitation of fucoidan is enormous, so that there is not enough action to remove alginic acid which can be fed non-economically and can be extracted together. In order to prevent this, it seems that an attempt was made to prevent the simultaneous extraction of alginic acid by lowering the pH to about 3.9-6.9 using organic acid, etc. in the extract aqueous solution. Has been required by law, it is that the pH and then extracted with 7.1 to 10.1 there is room for the multi-division of a larger improvement in possession of the problems in high alkaline conditions as referred to its use.

In addition, in the extraction of fucoidan from the US patent (US 6,573,250 B2) fucoidan content, calcium chloride is added to reduce the extraction amount of alginic acid, and fucoidan is extracted with alkali solution using sodium carbonate, acid is neutralized, and calcium chloride is added. Fucoidan-containing foods produced by killing cancer cells by adding an appropriate amount of fucoidan obtained by processing the temperature to 50-130 degrees Celsius and processing time 10-180 minutes to various foods by reducing alginic acid were also contained in kelp. Since the salt is extracted without separating and excluding it, the obtained fucoidan has a high salt and lacks a process for low molecularization and absorption so that there is a lot of room for development.

On the other hand, the processes described in Japanese Patent Laid-Open Publication Nos. 2002-220402 and 2002-262788, which appear to be quite advanced technologies, show that hydrogen peroxide is contained in a solution of 0.5-20 parts by weight of a brown algae in an aqueous solution of 0.1-1.0%. Add 15 minutes of stirring at 50-100 degrees Celsius (recommended time is 30-240 minutes), extract the extract, remove the low molecular materials using reverse osmosis membrane or ultrafiltration membrane, and recover the fucoidan. Fucoidan is making low molecular weight of 1000 Dalton,

Since all of the various extraction methods mentioned above are not described as a method of utilizing the extraction residues, they are considered to be disposed of and can be inferred to cause contamination. This is a method of causing contamination by only extracting fucoidan, for example, 30-40% of alginic acid in the remaining residues of brown algae, but not extracting and utilizing the resources sufficiently. In addition, in order to obtain fucoidan, a large molecular substance, which is one of the important substances, the method of concentrating and extracting and separating the extract by adding alcohol, is a method of causing further pollution since it is discarded without recovering and utilizing low molecular substances such as free amino acids. have.

Compared to this, a method that considers environmental pollution considerably and extracts material contained in brown algae better according to its function (European EU Patent WO 2005/014657 A1) separates and extracts the functional material through repetitive extraction, sedimentation and filtration. However, since the step of separating and using salt is not applied before extracting using organic solvent alcohol, it also contains the above-mentioned deficiencies of several methods, and the fucoidan and laminarin are recovered by extracting the alcohol extracted with hydrochloric acid again. However, the use of the functional material is not enough due to the acquisition and use of brown algae and chlorophyll, causing pollution. In each other stage, fucoidan, laminarin, and alginic acid are extracted and the final residue is treated with animal feed because it does not know that it is a cancerous substance.

None of the processes mentioned above does not apply the process of removing sodium and does not apply the method of using enzymes and organic acids to lower the molecular weight to better absorb the extracted and separated material. And pulverization to obtain semi-nanoparticle powder to produce a product that is well absorbed and functioning as a dry raw material, and does not apply, and continuously extracts and recycles bioactive substances from residues to prevent or reduce contamination. There is a lack of a back.

1. Brown algae, for example minerals in kelp, especially salty minerals, contain only about half the amount of potassium chloride as the main ingredient of normal salt, so they are considered to be very good seasonings for patients with hypertension or diabetes. Extractable to make available and another functional material; Fucoidan, laminarin, or alginic acid are pre-removed to contain very low sodium and develop product manufacturing processes that are beneficial to your health care.

2. Brown algae, for example, to separate and extract, concentrate and lower molecular weight and cause contamination of the functional material contained in kelp, without stopping in order to recover, dry, grind and increase the efficacy of the organic matter. Develop a process for obtaining and using semi-nano powders.

3. In order to manufacture raw materials of natural substances or pharmaceuticals that are not food raw materials, we develop processes to obtain pure substances rather than applying separation and column considering physical and chemical properties.

4. Develop a product that extracts bioactive substances from brown algae and utilize the remaining residues as dietary fiber, and develop a process that makes full use of brown algae.

The present invention is composed of seven steps and except for steps 6-7, step by step in order to extract the functional material of brown algae, concentrate, oligomerize, dry, pulverized to obtain semi-nanoparticles (powder), It is to develop a process that manufactures foods or supplies them as raw materials for foods and, if necessary, even pure products that can be supplied as raw materials for pharmaceuticals, but concentrates on all of them, regardless of molecular size, to reduce contamination. . In addition, the extraction and concentration process is well coordinated, and the process of extracting salt from brown algae and performing two-step alcohol extraction when concentrating it, extracting crude fucoidan when concentrating it, and extracting alginic acid when concentrating crude fucoidan. The process proceeds step by step without interruption, but alginic acid is adjusted to the extraction start time in consideration of the previous step.

Step 1: Seaweed (unsealed) brown algae (seaweed, seaweed, kelp, rhubarb and rhubarb), or dried brown algae, lifted from the sea, washed with a clean towel, washed with drinking water, and then sliced into approx. (500 liters or 5 tons depending on the quantity) and add 2 times the capacity of drinking water (10-20 times for dry matter) and stir at room temperature for half an hour to extract salt (preferably about 10-90 minutes). Filter and transfer to concentrator. Concentrate and sterilize under reduced pressure (-700mmHg) to prepare kelp- refined liquid (1-1 obtained) , or dry it with a dryer (frozen, sprayed or purate) to add seasoning salt ( 1-2 obtained ). Special salts (1-3 obtained ) are prepared by obtaining crushed quasi-nanoparticles several times (preferably 2-3 times).

Second step: Transfer the extracted residue to a large grinder (100 l or 300 l) and put the alcohol (95%) so that brown algae can be chopped (about 1 square centimeter) at high speed. Repeatedly carried out several times. Re-transfer alcohol to the extraction tank (adjust the alcohol concentration 75-90%) by adjusting 1-2 times the capacity of brown algae, extract it for 18-24 hours at room temperature while stirring and filter it and transfer it to the concentrator under reduced pressure (-700mmHg) Concentrate to remove all alcohol (recycle recovery), continue to concentrate, freeze-dried and washed repeatedly with alcohol (95%) 2-3 times, Chlorophyll (2-1 obtained) and brown fish (Fucoxanthin ( 2-) 2 ) to obtain the obtained product separately, to obtain the residue manititol ( 2-3 obtained ), and to obtain the pure product rather than using the column obtained material (2-1 and 2-2 obtained) Freeze or spray drying several times And supplies it to the.

Third step: 5-10 mM calcium chloride solution was added to the residue of the extraction tank, and the residue was heated with stirring to extract the crude crude fucoidan for 1 night (12-24 hours) at 70-100 degrees Celsius. Filter to transfer to concentrator (-700mmHg) Concentrate (about 5 times) and make enzyme (enzyme extracted from live abalone) or dilute vinegar solution (vinegar concentration: 0.6%) to make oligomer (vitamin) in concentrated oligomer solution After the sterilization treatment with oligosaccharides, etc. to prepare a drink (Drink), or dried to secure the supply as a kelp-fucoidan dry raw material. If necessary, laminarin (3-2 obtained water) is separated from the concentrate using the column, and purer fucoidan and namirarin are used as separate dry drying.

Fourth step: 20-25 mM potassium carbonate aqueous solution was added to the residue of the extraction tank twice the volume of the residue, stirred while heating (70-90 degrees Celsius) and extracted for 2-4 hours to obtain the alginic acid (fourth obtained ) Transfer to concentrator (-700mmHg) Concentrate (about 5 times) to neutralize with organic acid (vinegar or lactic acid) and adjust with diluted vinegar liquid (vinegar concentration: 0.6%) by adding enzyme (enzyme from live abalone) or vinegar. Heated to decompose 3-4 hours at 100 degrees Celsius to make oligomer, precipitate as needed alcohol (95%), recover the separated alginic acid, and dry several times to obtain semi-nanoparticles for use as dry raw materials. Prepare, but remove the precipitate alginic acid and recover the alcohol in vacuo and continue concentrating to obtain the alginic acid oligomer to sterilize with addition of vitamins and oligosaccharides to prepare a kelp-fiber drink.

Step 5: Take out the residue of the remaining extraction tank and administer the dried kelp powder to adjust the moisture content to 35-45% and mix well with vitamin C, etc. Use a granulator to make kelp granules (obtained from 5-1) or refiller To prepare a kelp-ring (5-2 obtained) . Also, kneaded powder of kelp powder 5-10% in flour and wet residue, and then kneading the granulator first and using a dry noodles, kelp-noodles (-5-3 obtained) or kelp- homemade ratio (5-4 obtained) ) to apply the process to prevent contamination inhibiting the generation of waste produced.

Step 6: The crude fucoidan extract is also extracted with laminarin, which may be obtained by separating a polystyrene column (Amberlite XAD-2 or Cavilink) or a DEAE-Sepadex A-25 column, which is used in the latter. The solvent is column chromatographed while gradually increasing the concentration of sodium chloride (salt) to 0.1M-3M in 0.01M hydrochloric acid, and laminarin (3-2 obtained) is separated at low concentration (salt). The fucoidan (3-3 obtained water) can be separated at high concentrations to obtain a relatively pure material.

When separating into a styrene column, the concentrate is loaded onto the column. Laminarin is adsorbed to the styrene filled in the column, and fucoidan comes out immediately. Narrin is recovered by releasing 15-20% alcohol.

Each solution containing the material thus obtained is neutralized with an alkaline solution (aqueous potassium carbonate solution) and then dialyzed in refined water overnight (1-24 hours) for desalination, or after the ethanol is removed in vacuo, each is used as a base for preparing oligomers. Use or dry to supply.

Step 7: Low molecular weight and quasi-nano-ization: The step of preparing the oligomer extracts, separates and concentrates the polymerase in the live abalone gut, immobilizes it in polystyrene, and prepares the oligomer using the packing in the column. Two methods of preparing oligomers by acid decomposition of 2-4 hours at boiling temperature by administering 1/10 of the vinegar (6%) of a polymer (fucoidan, laminarin, or alginic acid) are used. The quasi-nano-nanolysis process collects the semi-nano powder by the use of a large number of plastic bags on the outside of the bag used for several milling operations (preferably 2-3 times) and powder collection.

end. Enzymatic digestion method: Remove the internal organs of the fresh raw abalone, put it in the blender, crush it using 0.1M acetate buffer (pH6.5) water, filter it to remove suspended matter, and first precipitate in 40% ammonium sulfate solution to remove the polymer. The precipitate was then centrifuged with an aqueous solution of 85-90% ammonium sulfate to obtain a centrifugal separation. The dialysis salt was removed from the brine, concentrated, immobilized in polystyrene, filled in two columns (3 X 50 cm), and then decomposed (fucoidan, ramie). Nalin or alginic acid) is a method of preparing oligomers by applying a pump pressure one by one. The contact time between enzyme and polymer is 30-90 minutes, the reaction temperature is 20-40 degrees Celsius, and the salt concentration is 100mM. It is preferable to use.

I. Acid Decomposition Method: In consideration of the capacity of the polymer extracted and concentrated at each stage, 1/10 volume of vinegar (6%) is added, stirred with heating (90-100 degrees Celsius), and acid-decomposed for 2-4 hours depending on the concentration. The oligomer is produced, which is concentrated in vacuo (-700mmHg) to remove acetic acid and to produce an additional concentrated oligomer according to the concentration. Alginate, which is dry and insoluble in water, is processed several times until the semi-nanoparticle powder is ground.

All. Semi-nanoparticle powder manufacturing process: Completely dried raw materials (spice salt, fucoxanthin or alginic acid) are pulverized using primary shreds and second and third milling are carried out using second 100 mesh shreds. A large plastic bag is added to the outside of the collecting cotton bag to collect the semi-nano powder. Even after crushing and collecting two 100 mesh blades, the particles remaining in the cotton bag are repeatedly obtained by repeatedly pulverizing or blowing the nanopowders through air blowing. This is a structure in which three vertical partitions separate the powder that enters with the wind from separate inlets by the weight and size of the particles by the wind, and the wind collects large particles in the adjacent compartment according to the size and weight of the particles. The third column, the farthest, captures the semi-nanoparticles. Thus, the powder collected in the first and second bins are subjected to repeated blowing separation process through crushing again to obtain semi-nanoparticles.

The following examples are described in detail below to aid in understanding and to clarify methods for extraction, concentration and low molecular weight, and the like, but the present invention is not limited thereto.

<Example 1>

Step 1: Remove 10 kg of dried kelp with a clean towel, cut it into 10cm size using small bean sprouts, put it into the extraction tank (500 liters dry), add 200 liters of tap water, and extract salt for 1 hour while stirring at room temperature. Filtered by a filter placed under the inside of the extractor and transferred to the concentrator. Warmed to 50-60 degrees Celsius, concentrated for 18 hours under reduced pressure (-700mmHg), and tasted very salty. Could be obtained. Lyophilization of 7 liters of this solution yielded 546 g, and if dried, 2.73 kg of dry matter could be obtained. The yield is expected to be 27.3% based on 10kg kelp.

<Example 2>

Stage 2 operation: The remaining residue from the first stage extraction is taken out from the extraction tank and put into a 100 liter container, which is filled with a small amount (about 5 kg) in the blender and the alcohol (95%) is immersed in the kelp. It took 2.5 hours to continue the work of crushing (about 1 square centimeter) at high speed for 1 minute and then re-transmitting it to the extractor. Add 100 litres of alcohol to this, and stirring it at room temperature for 24 hours, extract chlorophyll, brown algae and mannitol, which are substances extracted by alcohol (75-85%), and filter them to reduce the pressure at 40-45 degrees Celsius. (-700 mmHg) was concentrated to remove all the spirits, the temperature was continuously concentrated to 50-60 degrees Celsius and concentrated to 24 liters (pH: 6.5, Brix: 10). The freeze-drying of 2 liters of this concentrate yielded 200 g, and if dried, 2.4 kg of dry matter could be obtained. This yields 24% based on 10 kg of kelp.

Barley powder, brown rice powder, carrot powder, soybean powder and vitamin C are mixed with 20 liters of concentrate before drying to prepare granules. Nutritional analysis showed that 380 Kcal, 100% carbohydrate, 2% protein, 0.5% fat, A product of 190 mg of sodium was obtained.

1 g of the dried product was extracted three times with 100 ml of alcohol (95%), concentrated to 3 ml, and loaded into a column (22 mm x 240 mm) filled with silica gel 60 (0.063-0.2 mm) to be first coated with ethyl acetate. green chlorophyll was inoculated with acetate, followed by ethanol to red-brown fucoxanthine, and the antioxidant activity was estimated. The discoloration of 1,1-diphenyl-2-picrylhydrazyl (DPPH) was measured at 517 nm using spectropodometa and compared with vitamin C. Fucoxanthine was found to be slightly stronger than vitamin C. Alcohol (95%) The remaining material was not identified as Mannitol using NMR, and the dry weight was 0.75 g. Therefore, the yield of 18% could be expected as 1.8 kg of mannitol was contained in 10 kg of kelp.

<Example 3>

3rd step work: add 200 liters of drinking water to the residue shank after the above 1st and 2nd step extraction, add 150g of calcium chloride to keep alginic acid together with the mixture, and then warm it with 70-90 degrees Celsius and stir for 24 hours. The fucoidan was extracted, filtered and transferred to a concentrated tank, concentrated under reduced pressure (-700 mmHg) to obtain 40 liters (pH: 7.3, Brix: 1.0). The freeze-drying of 2 liters of the concentrate yielded 18.4 g, and 368 g were obtained if the whole was dried. A yield of 3.68% was expected based on 10 kg of kelp.

The concentrate was sterilized by adding a certain amount of fructose, vitamin C, and B2 to 35 liters of concentrate, and 50 ml (700 bottles) of kelp-fucoidan beverage was filled in a glass bottle. Nutritional analysis: calories per 100 ml: 29 Kcal, carbohydrate: 7 g, protein: 0.08 g, fat: 0.02 g, ash: 0.28 g, sodium: 41.3 mg of the beverage could be obtained.

<Example 4>

Fourth step operation: 200 liters of drinking water and 500g of potassium carbonate were added to the residue shank after the extraction steps 1, 2, and 3, and the mixture was heated to 70-90 degrees Celsius, stirred for 2.5 hours, filtered and transferred to the concentrator. 700 mm Hg) was concentrated to obtain 70 liters (pH 10.2, Brix: 1.2). 20 ml of lactic acid (90%) was added to 2 liters of the concentrate, and a gel was formed at a pH of 4.5. The freeze-drying resulted in 56.1 g, which is 1.96 kg if the whole is dried. A yield of 19.6% is expected.

To neutralize the 35 liters of concentrate, add lactic acid so that the mixing pH is 6.5 and add a certain amount of oligosaccharide, vitamin C, and B2 to sterilization (15 minutes at 121 degrees Celsius). Young nutrient analysis of kelp-fiber drink yielded 35 Kcal per 100 ml, carbohydrate: 9 g, protein: 0.15 g, fat: 0 g, ash: 0.64 g, sodium: 37.5 mg / 100 g.

<Example 5>

5th step work: 11kg of the wet residue, which was subjected to the extraction operation, 20kg of kelp dry powder was added, and vitamin C was added and mixed well to prepare and dry granules. 21.4kg of calorie analysis resulted in calories: 162.3Kcal / 100g, Granules of 57.4% carbohydrate, 11.6% crude protein, 1.9% crude fat, and 30.9 mg / 100 g sodium were obtained.

In addition, 10 kg of wet residue was mixed with 20 kg of flour containing 6% of kelp dry powder, and passed through a granulator. Then, noodles were prepared by drying with a dry noodle and analyzed by nutrients. 324.2 Kcal / 100 g, carbohydrate 67.8%, protein 14.8%, fat 21 kg of dried noodles of 1.8% and 1,078 mg / 100 g of sodium were obtained.

<Example 6>

The crude fucoidan 2 liter of neutral (pH 7.3) prepared in Example 3 was immobilized and purified from polyhedron by polystyrene, and the column (3 x 50 cm) was packed in a column (3 x 50 cm) for 1 hour at 25-30 degrees Celsius. Precipitation with the same volume of alcohol resulted in about 1/5 of the control, which was interpreted as the breakdown of the polymer fucoidan into small molecules of oligomer or monomer. . In addition, 0.2 liter of vinegar (6%) was added to 2 liters of crude fucoidan and decomposed with stirring for 3 hours at 90-100 degrees Celsius, and the concentrated vinegar was blown out and concentrated to 1 liter of concentrated alcohol (95% As a result of precipitation (refrigerated), a precipitate of about 1/5 of the control was obtained.

<Example 7>

Vinegar was added to 2 liters of the alginate concentrate obtained in Example 4 to pH 6.5, and 0.2 liters of vinegar (6%) was added thereto, followed by heating (90-100 degrees Celsius) and stirring for 3 hours. After the concentration was concentrated under reduced pressure (-700mmHg), the acetic acid was blown out, concentrated to make 1 liter, and precipitated with the same volume of alcohol (95%). As a result, about half of the aggregates were observed. In addition, 2 liters of concentrate was enzymatically digested using a polystyrene column in which enzyme was immobilized. After precipitation refrigeration, the precipitate was compared with the control, and as a result, the aggregate was found to be about 1/3.

<Example 8>

Step 1 Extraction Concentrated Dried Cataract Salt is a white powder that absorbs moisture in the air. The evaporation of the moisture in the oven is followed by primary, secondary and tertiary crushing, and the semi-nanoparticles collected in the outer plastic bag are collected. Yield of% was shown.

Two-Stage Extraction Concentrates Dry chlorophyll and fucoxanthin are reddish brown powders that absorb moisture well, so they evaporate moisture in the oven, pulverize primary, secondary and tertiary and obtain semi-nanoparticles collected in a plastic outer bag outside the cotton bag. Yield of% was shown.

Three-Step Extraction Concentrate The dried fucoidan is a milky powder like cotton, which does not absorb moisture. It is dissolved in 0.1M citrate buffer (pH6.5) water to produce oligomeric oligomers and dried to yield 75% yield. .

Four-Step Extraction Concentrate The alginic acid produced a light gray dense honeycomb solid that resembles a cuttlefish, which did not absorb moisture and could easily be pulverized. The primary, secondary and tertiary mills were collected and collected in a plastic outer bag outside the cotton pouch. The quasi-nanoparticles were obtained, yielding 73%. The freeze-dried alginic acid was dissolved in water and digested with diluted vinegar (0.6%) at 95-100 degrees Celsius for 3 hours, but it did not show any difference from the control when precipitated with alcohol. It was confirmed that alginic acid is an efficient and economic method of acid decomposition before lyophilization.

1. Brown algae, for example, can be used not only to produce natural seasonings, but also salt and special salts.

2. The residue can then be extracted and separated to the next level of alcohol (~ 85%) to produce chlorophyll and fucoxanthin-containing products and mannitol.

3. Continue to the next step to obtain fucoidan and laminarin content by calcium chloride-containing hot water extraction concentrated to prepare the fucoidan or laminarin oligomer by enzyme or acid treatment.

4. The next step is to extract and neutralize the alginate with alkaline hydrothermal water to produce oligoalginate (oligo-alginic acid) by enzyme or acid treatment.

5. Continue to mix the residue with the brown algae powder to produce granules or pill, and if necessary, mix it with flour containing kelp powder to make brown algae noodles or sujebi. You will have the result of utilizing it efficiently.

6. By controlling the work of extractor, concentrator and decomposer well, this step-by-step operation is to concentrate the 1st step extraction in the concentrator during the 2nd step extraction, and in the 3rd extraction operation, the 2nd step extraction is concentrated and the 1st extraction is concentrated. It can save manpower and time by continuous processing without disassembly and transfer interruption to product processing machine.

7. If necessary, the material is separated and dried to manufacture powders and semi-nanoparticles (powders), so that it is possible to supply raw materials for health foods and pharmaceuticals, experimental raw materials for natural products, and standard products. Enhancing the absorption of the human body increases the efficacy of the product, and the drying life can increase the shelf life and shelf life.

Claims (2)

Taking into account the properties of the bioactive substances in brown algae, stepwise extraction, concentration, low molecular weight, drying, pulverization and quasi-nanoparticleization, and all products usefully without waste do not cause contamination A method of extracting and obtaining bioactive substances by applying a continuous extraction process, some or all processes. A material produced according to claim 1 and a product containing the material.