KR20030082619A - Compositions for improving immune activity - Google Patents

Abstract

The present invention relates to a composition for enhancing immune activity, 30-50% by weight of chitooligosaccharide, 25-35% by weight of beta glucan, 5-15% by weight of fingering mushroom fruiting body extract, Ganoderma lucidum fruiting body powder 2-10% by weight, The composition for enhancing immune activity, comprising 2-10% by weight of vitamin and mineral mix, 0.5-5% by weight of vitamin C and 0.5-5% by weight of biozymes, has an excellent immunity enhancing effect and cholesterol reducing ability, and especially every day without side effects. It can be taken and is effective in preventing immunity deterioration and improving human healing.

Description

Composition for improving immune activity

The present invention relates to a composition for enhancing immune activity, and more particularly, has excellent anti-complement activity, T ?? B cell proliferation ability, and macrophage activity ability, and especially, can be taken daily without side effects, thereby preventing a decrease in immune function and naturally It relates to a composition effective for increasing the healing power.

It is human self-healing that can overcome the natural action of the body without any action against disease. The increasing number of diseases in modern society is due to the deterioration of human self-healing. However, modern society has a tendency to rely on medicine even for minor symptoms, and the self-healing power is gradually weakened.

Immunity is what distinguishes me from others, and immunity is a self-defense force that protects the body from internal and external enemies. It is the "immune system" that plays a role in maintaining health when external enemies such as bacteria and viruses, and internal cells, which are abnormal cells among new cells created by dead cells or gene cloning, are eliminated. Therefore, it is natural that the disease is easily affected when the immune function is lowered. In addition to aging, immune function is also lowered.

Immunity is largely divided into innate immunity that has been born since birth and acquired immunity that is acquired by adapting to life.

Innate immunity, also known as "natural immunity," is a nonspecific reaction to antigens and does not have a special memory function. Innate immune systems include skin, mucous tissues, acidic stomach acid, and complement that are present in the blood to block antigen invasion. Cells include macrophages responsible for phagocytosis, polymorpho nuclear leukocytes, and K cells that can kill infected cells. In fact, most infections are protected by this innate immunity.

In addition, acquired immunity, also known as "acquired immunity" and can remember the first invasion of the antigen, and when it invades again, it has a characteristic that can effectively remove the antigen effectively serves to reinforce innate immunity. The commonly used definition of immunity says this. This acquired immunity is understood to be divided into humoral immunity and cell-mediated immunity for convenience.

Humoral immunity is differentiated after B lymphocytes recognize antigens to secrete antibodies, which are mainly used to remove infected bacteria. Antibodies exist in body fluids and are composed of glycoproteins called immunoglobulins (abbreviated as Ig). There are kinds of IgG, IgM, IgM, IgA, IgD, IgE, etc., each of which performs a unique function, and some functions overlap. IgA antibodies are characterized by their delivery to the fetus via the placenta. This immunity is called maternal immunity, and because of this, it does not infect well for several months after birth.

Cellular immunity plays a role in T lymphocytes derived from the thymus, which recognizes antigens to secrete lymphokine or directly kill infected cells. Secreted lymphokines may also activate phagocytosis to help phagocytosis. Such cellular immunity primarily functions to remove cells infected with viruses or bacteria that can grow in cells. Acquired immunity can be obtained by immunization of a pathogen or its toxin with an immunogen, and such immunity is called artificial immunity. E. Jenner first discovered the head-to-head method in this way and laid the foundation for immunology. Various artificial immunity methods are being developed today and are being applied to prevent cancer, acquired immune deficiency syndrome (AIDS) and hypersensitivity reactions.

In general, when the immune function is reduced, allergic reactions (allergy, hypersensitivity) are caused, and allergic reactions such as allergy can be divided into four types based on expression time and reaction type. Type I (immediate) is typical of most allergic reactions, including symptoms of asthma, seasonal or perennial rhinitis, allergic sinusitis, conjunctivitis, food and drug allergies, atopic dermatitis, hives, and bee stings. Reaction and the like.

Type II (antibody involvement) causes cytolytic or inflammatory reactions of humoral immunity. Symptoms include hemolysis of red blood cells due to inadequate transfusion, and neonatal hemolytic disease (for Rh-mothers).

In addition, type III is caused by a reaction by antigen-antibody binding or by an antigen of small molecular weight. Causes acute glomerulonephritis, irritable pneumonia.

Finally, type IV (delayed) is a type of cellular immune response induced by T lymphocytes and macrophages that triggers an important response in response to invasion of microorganisms such as tuberculin, rejection, and viruses of Mycobacterium tuberculosis.

Most food-related allergies belong to type 1 of hypersensitivity reactions. The representative expression is anaphylaxis. Anaphylaxis is characterized by irritable shock symptoms such as blood pressure lowering by small amounts of antigens such as peanuts, fish such as blue fish, chicken, chocolate, mushrooms, and flour foods, airway contraction, increased secretion of bronchial mucosa, edema, and smooth muscle contraction. This is because antigens absorbed through the mucosa bind to immunoglobulin E antibodies already bound by mast cells, and mast cells secrete vascular active substances such as histamine by binding signals. Treatment is to avoid allergic foods and antihistamines. As such, the immune function plays an important role in the human body, but if the abnormality has a fatal effect on the human body, there is a problem that the natural healing power is lowered due to wrong eating habits or lifestyle of modern people.

For the reasons described above, various immune enhancing therapeutic agents have been developed and used to overcome the problem of impairment of immune function.

However, when taking a separate therapeutic agent for a long time, there is a disadvantage that the problem of the side effects is large because the difference in immunity for each individual. In particular, the immune disease is more important than the treatment, the reality is that it is difficult to take the currently available treatment for the prevention. Therefore, there is a need for a method capable of preventing and treating immune dysfunction without taking a separate immune enhancing agent having potential side effects.

The preferred method to meet the needs as described above, is to mix the food-derived ingredients that have been previously ingested to ensure stability and to obtain an effect of improving immune function in the process of taking with food without any side effects.

Accordingly, the technical problem to be achieved by the present invention is that when the immune function is weakened as described above, the effect of enhancing the immune activity is excellent, and when the immune function is deteriorated for a long time, there is an effect of increasing the natural healing power. In particular, it is possible to take daily without side effects to provide a composition for enhancing immune activity effective in preventing immunity lowering.

Figure 1 shows the results of measuring the anti-complement activity of the composition prepared according to Example 1 of the present invention,

Figure 2 shows the T ?? B cell proliferation measurement results of the composition prepared according to Example 1 of the present invention,

3 and 4 show the results of measuring the macrophage activity of the composition prepared according to Example 1 of the present invention.

In order to achieve the above technical problem, in the present invention, 30-50% by weight of chitooligosaccharide, 25-35% by weight of beta glucan, 5-15% by weight of fingering mushroom fruiting body extract, Ganoderma lucidum fruiting body powder 2-10% by weight, vitamin mineral mix 2-10% by weight, 0.5-5% by weight of vitamin C and 0.5-5% by weight of biozymes.

The composition for enhancing immune activity is 42% by weight of chitooligosaccharide, 29% by weight of beta glucan, 10% by weight of fingering mushroom fruiting body extract powder, 7% by weight of Ganoderma lucidum fruiting body powder, 7% by weight of vitamin mineral mix, 3% by weight of vitamin C and It is preferred to include 2% by weight of biozymes.

The composition for enhancing immune activity of the present invention can be used for the treatment of cancer, the prevention or treatment of bacterial infections or viral infections.

The composition for enhancing immune activity of the present invention contains chitooligosaccharide, beta glucan, fingerling mushroom fruiting body extract powder, ganoderma lucidum fruiting body powder, vitamin mineral mix, vitamin C and biozymes.

Hereinafter, each component constituting the composition for enhancing immune activity of the present invention will be described in more detail.

Chitooligosaccharides have high water solubility and are excellent in body absorption, so it turns out to be a wide range of high functional bioactive substances such as immune enhancement, antibacterial action and calcium promoting action. Increasing the activity of lymphocytes contributes to inhibition of proliferation of cancer cells. In addition, ingesting chitooligosaccharides activates liver function, promotes bile production, and breaks down fat to produce the energy needed. Taking chitooligosaccharides before and after drinking has the function of relieving hangovers by promoting the production of enzymes that break down aldehydes that harm each organ. And since chitooligosaccharides are released in combination with chlorine ions, they are effective in improving hypertension, as well as blocking the absorption of cholesterol by absorbing and releasing cholesterol in the body, dissolving and discharging blood clots in blood vessels, and stimulating the autonomic nervous system. Peripheral blood vessels dilate to have a good effect on hypertension. It also activates beta cells in the pancreas, helping to produce insulin, thereby lowering blood sugar levels by increasing the sensitivity of the membrane insulin receptor.

As described above, taking chitooligosaccharide increases the body's immunity by 4 ?? 5 times, helps the antibody formation, and enhances the cellular activity of attacking only cancer cells in the human body 10 times after administration. The content of such chitooligosaccharide is preferably 30-50% by weight based on 100% by weight of the total composition. If the content of chitooligosaccharide is less than 30% by weight, there is a problem in that the formation of the antibody helps to enhance immunity or exhibits antimicrobial activity, and when the content exceeds 50% by weight, a certain amount of energy is ingested. It is not desirable because of the lack of a balanced supplement.

Beta glucan (??-Glucan) constituting the composition for enhancing immune activity of the present invention is an important component that cannot be produced in the human body, binds to macrophage receptors to activate macrophages and immunizes normal human tissue. It activates the function, inhibits the proliferation and recurrence of cancer cells, and promotes the production of interleukin and interferon, which activate immune cells. Active beta glucan enters the body where cancer cells are located and produces cytokines (Cytokine) to support the activity of immune cells, T cells and B cells, thereby activating the immune function of cellular tissues. And instead of directly attacking and reducing cancer cells, by strengthening the immune system of the entire body indirectly shrinks cancer cells. Therefore, if a cancer patient performs immunotherapy, it exhibits strong anticancer activity, and if taken by other members of the public, it is possible to prevent immunity from other diseases, such as preventing adult diseases and preventing colds.

The content of the beta glucan is preferably 25 to 35% by weight based on 100% by weight of the composition. If the content of the beta glucan is out of the above range there is a problem that does not effectively enhance the activity of the macrophages to activate the immune function of the cell tissue is insignificant.

The Cloudy Mushroom is a fungus belonging to the Democratic Fungus Hole family, called Glucan, a substance that forms the cell wall of the mushroom. It is commonly called Polysaccharide-K (PSK). Therefore, PSK has little toxicity to normal cells and helps the immune system in addition to anticancer efficacy. To date, the mechanism of action of PSK inhibits tumor growth through a process very different from common anticancer drugs such as interferon. Interferon directly inhibits the growth and division of cells to inhibit the growth of cancer cells, while PSK eliminates cancer cells through enhanced immune function. Interferon and similar anticancer drugs have the side effect of inhibiting other normal cells to kill cancer cells.However, PSK extracted from finger mushrooms does not directly show cytotoxicity and removes abnormal tissues including cancer cells. Inhibits the growth of cancer cells by increasing the activity of T-Cell known as cells.

Its fruiting body extract powder using the fingering mushroom can be obtained according to the following procedure.

First, the fingering mushroom fruit body is cut or pulverized as it is or a suitable size to be immersed in the alkaline solution. It is then washed with water or the alkaline solution is removed and then treated several times at 110 to 140 ° in the presence of pressurized steam. The processed fingerling mushroom fruit body is added to water and boiled for 20-30 minutes to extract hot water components. The resultant is then concentrated and lyophilized to powder.

In the present invention, the fingering mushroom fruit body extract powder is preferably 5-15% by weight with respect to 100% by weight of the composition, when the content of the fingerling fruiting body extract powder is out of the above range, the T-cell activity is weakened to increase the immunological activity. It is not preferable because the action to weaken the ability to inhibit the growth of cancer cells.

Biozymes activates tissues and cells to facilitate all metabolic functions of the human body and promote the production of various hormones. If you eat more food and eat more medicines or harmful additives, the amount of enzymes in your body will be less or slow down. And constitution improvement, health promotion, human metabolic function, digestive function is effective. Herein, the content of biozymes is preferably 0.5-5% by weight. If the content of the biozymes is out of the above range, there is a problem that the digestion absorption rate is lowered to facilitate the metabolic function.

Ganoderma lucidum, which is one of the components of the composition for enhancing the immune activity of the present invention, has a generic name such as Bulchocho, Ganoderma lucidum, and perennial mushroom, and grows by attaching only half-shade with a half moon type as a parasitic fungi attached to rotten tree trunks of various hardwoods. . The anticancer activity of the hot water extract extract against mouse sarcoma 180 was found to be glucan combined with 2/10 of tumor complete regression rate, 77.8% tumor suppression rate, and polysaccharide glucose showing antitumor effect, which is effective for Sarcoma 180 glioblastoma. It is known that the administration of 1mg to kill cancer tissues in a short time. The anticancer effect of this plant polysaccharide is known to cause a protective action (immunity, etc.), unlike an anticancer agent that acts as a cytotoxic agent to the human body. And many of these polysaccharides have the strength to almost stop transplantation cancer in mice, even in trace amounts of only 1mg. Therefore, as the fourth secret weapon, manor recently appeared in the spotlight in the operation of the biggest enemy of cancer common to mankind. This is based on the body's system of immunotherapy that has the human body's power to suppress cancer, and excludes foreign substances such as viruses and bacteria from outside the body. According to the recent analysis results by Chinese scientific officials, alkaloids, sterols, and phenolic acids are extracted, and they have strong heart, soy sauce protection, and soothing effects. When these ganoderma lucidum is used, it can be used to treat adult diseases such as insomnia, hypertension, diabetes, and arteriosclerosis.

In the present invention, the powdered ganoderma lucidum fruiting body is used, and to prepare it, the blanching mushroom ganoderma lucidum (blanching) after enzyme deactivation and hot-air dried at 60-70 ?? so that the moisture is about 10%. This was pulverized and treated with 100mesh to make and use Ganoderma lucidum fruiting body powder.

The content of the Ganoderma lucidum fruiting body powder is preferably 2-10% by weight based on 100% by weight of the composition.

Vitamin C forms interferon and promotes the production of T lymphocytes that attack cancer cells. Involved in the synthesis of immunoglobulins and the synthesis of antihistamines, the immune system is strengthened, and cholesterol is broken down into bile acids to help prevent disease and prevent pharmacological effects. It is involved in the synthesis of growth hormones and the synthesis of intelligent regulators, and is involved in metabolism, which is the source of phagocytosis of white blood cells. The suitable content range of vitamin C here is 0.5-5% by weight relative to 100% by weight of the composition.

Vitamin mineral mix used in the present invention is a mixture of vitamins and minerals. Vitamins themselves are not biological energy sources, but vitamins are absolutely necessary for living things because they are involved in making energy sources into energy. Minerals, like vitamins, do not generate energy, but are also important for organizing the human body, regulating the activity of the heart, nerves and muscles, regulating the formation of hemoglobin and heart rate, as well as transporting oxygen and helping tissue respiration. And the content of the vitamin mineral mix is preferably 2-10% by weight relative to 100% by weight of the composition.

The composition for enhancing immune activity according to the present invention having the composition as described above is in particular 42% by weight of chitooligosaccharide, 29% by weight of beta glucan, 10% by weight of fingerling fruiting body fruit extract, Ganoderma lucidum fruiting body powder 7% by weight, vitamin and mineral mix 7 It is particularly preferable to have a composition of% by weight, 3% by weight of vitamin C and 2% by weight of biozymes in terms of anti-complement activity, macrophage proliferation ability and the like.

The composition for enhancing immune activity of the present invention comprising the components as described above is a mixture of the respective components reported to increase the immune activity, such as chitooligosaccharide, beta glucan, finger finger mushroom, and the immune activity is significantly enhanced It has a synergy effect.

Meanwhile, chitooligosaccharide, beta glucan, fingerling mushroom fruiting body extract powder, ganoderma lucidum fruiting body powder, vitamin mineral mix, vitamins and biozymes used in the present invention have been used as foods or drugs for a long time. Can be taken without.

The composition of the present invention may be formulated by further mixing the carrier or excipient or diluting with a diluent. Examples of suitable carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. The composition may further include fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers, preservatives and the like. Compositions of the present invention may be formulated using methods well known in the art to provide rapid or delayed release of the active ingredient after administration to a mammal. The formulations may be in the form of tablets, powders, pills, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders and the like. The compositions of the present invention can be administered via several routes including oral, transdermal, subcutaneous, intravenous or intramuscular.

The dosage of the composition according to the present invention varies depending on the condition of the subject, but is usually divided into 2-3g / 60kg (weight) per day, 2-3 times a day is preferable to age, sex, weight, health Can be increased or decreased accordingly.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the following examples.

Example 1 Preparation of Composition for Enhancing Immune Activity

Chitooligosaccharide 42g, beta glucan 29g, 10g of fingerling fruit body extract powder, Ganoderma lucidum fruit body powder 7g, vitamin mineral mix 7g, vitamin C 3g and biozymes 2g to prepare a composition for enhancing immune activity.

20 g of the composition for enhancing immune activity and 200 ml of distilled water were mixed and extracted for 12 hours at room temperature. The resultant was then filtered (Wattman No. 2) and lyophilized to prepare a sample (referred to as "Immunomax").

Experimental Example

The anti-complement activity, T-B cell proliferation and macrophage activity of the sample immunomax prepared according to Example 1 were measured according to the following method. At this time, in order to compare the characteristics with the sample Immunomax of the composition of the present invention, biozymes, ganoderma lucidum fruit body powder, vitamin C, fingering mushroom fruit body extract, glucan s, chitooligosaccharide, vitamin mineral mix in the same manner to prepare a sample Anti-complement activity, T-B cell proliferation and macrophage activity were also examined for each.

(1) measurement of anticomplement activity

Anti-complement activity of the sample immunomax prepared according to Example 1 was measured according to the Meyer method (Kabat and Mayer, 1961) described below.

50 μl of NHS (Normal Human Serum) and GVB ⁺⁺ (gelatin veronal buffered saline, pH 7.2) and 50 μl each were reacted at 37 ° for 30 minutes, and then 350 μl of GVB ⁺⁺ was added to the reaction solution. 10 to 160 times of dilution, 750 μl of GVB ⁺⁺ and positive sensitized blood cells (10 ⁸ cell / ml) were added to the solution for 1 hour, followed by PBS (phosphate buffered saline, pH 7.4). 2.5 ml each was added and the absorbance of the supernatant after centrifugation was measured at 412 nm.

On the other hand, the control group was measured with no sample under the same conditions as described above, and the anti-complement activity was inhibited by 50% of total complement hemolysis (TCH ₅₀ ) compared to the control group (inhibition of TCH ₅₀ or ITCH ₅₀ ). Represented by. In order to assay the exact activity of the sample, an anti-complement polysaccharide having a known titer was used as a standard.

ITCH ₅₀ (%) = (Control TCH ₅₀ -Sample TCH ₅₀ ) / Control TCH ₅₀ × 100

(2) T cell and B cell proliferation capacity measurement

The T cell and B cell proliferation ability of the sample prepared according to Example 1 was measured according to the MLR (Mixed Lymphocyte Reaction) method, which will be described below.

Lymphocytes were obtained by spleen extraction from ICR mice, red blood cells were removed, and washed 2-3 times with RPMI-1640 medium to adjust the cell number to 5 × 0 ₆ cells / ml. 180 μl of the obtained suspension is dispensed into a 96 well plate, 20 μl of sample is added thereto, followed by incubation for 96 hours at 37 ° C. in a 5% CO ₂ incubator. Was measured.

(3) macrophage activity measurement

Macrophage activity is measured according to the following method.

1) NO ₂ generation

TG (Thioglycollate) in the mouse abdominal cavity was injected 3 days later, macrophages were recovered from the abdominal cavity, and the number of cells was adjusted to 1 × 0 ₆ cells / ml using DEME medium. After dispensing this in a 96 well plate, take 100 µl of the cell culture supernatant from each wall of the incubator mixed for 24 hours, and react with the same amount of Griess reagent for 10 minutes at room temperature. The degree of NO ₂ production was measured at an absorbance of 540 nm in an ELISA plate. NO ₂ quantification uses a standard curve of NaNO ₂ by step dilution.

2) macrophage activity

TG (Thioglycollate) in the mouse abdominal cavity was injected three days later, the macrophages were recovered from the abdominal cavity, and redispersed in RPMI-1640 medium so that the number of macrophages was 1 × 0 ₆ cells / ml. Disperse RPMI-1640 medium and cells containing FBS on each wall, incubate in 37 ° C, 5% CO ₂ incubator for 24 hours, add Triton X-100 to macrophages After dissolving the cell membrane, p-nitrophenyl phosphate was added as a substrate of lysosome phosphatase, which was then secreted in an acidic state for 1 hour, and borate buffer was added to stop the reaction. After measuring the absorbance at 405nm with an ELISA reader (reader).

The anti-complement activity measurement results described above are shown in FIG. 1.

Referring to FIG. 1, the activity of most samples was lower than that of the positive control group LPS (lipolysaccharide). Among them, Immunomax, the composition of the present invention, had the highest activity, and the fingerings of the fruiting fruit extracts of the fingerling appeared to be slightly active, and other samples were almost insignificant. In general, the water-soluble polysaccharide component of Ganoderma lucidum fruit powder has a high complement activity, but the activity was not shown in this experiment. The reason seems to be due to the fact that the water-soluble substances useful for complement activity were not available because the fruiting body powder was used instead of the extract. In contrast, the case of the fingerling fruit fruit extract powder showed similar activity to the reported data, which may be due to the useful water-soluble component extracted from the fruiting body.

The T.B cell proliferation measurement results are shown in FIG. 2. Referring to this, as a result of measuring the proliferation capacity of the spleen-derived T-B-cells using the MLR method, the immunomax, which is the composition of the present invention, was the highest, and the chitooligosaccharide-derived substance was the highest in each sample. appear. There was also no significant difference, but it was slightly higher at low concentration.

3 and 4 show the measurement results of the macrophage activity. As can be seen from this figure, the chief components of activating macrophages were found to be chitooligosaccharides and fingering mushrooms. The NO ₂ production ability showed that chitooligosaccharide had similar activity to the immunomax of the composition of the present invention, and the fingering showed slightly higher activity than other samples. Phosphorase (Phosphatase) production ability was also slightly active in the fingerling fruiting body extract powder, and chitooligosaccharides showed a high result.

As described above, the immunomax, the composition of the present invention prepared according to Example 1, was found to have superior immunity to each individual component. In addition, all samples are involved in the action of activating macrophages, but in particular, fingering mushrooms and chitooligosaccharides have the greatest effect, and among them, chitooligosaccharides are mainly involved.

The composition for enhancing immune activity of the present invention has excellent immunity-enhancing effect and cholesterol-lowering ability, and is particularly effective in preventing immunity deterioration and improving the healing ability of the human self since it can be taken daily without side effects. In addition, the composition for enhancing immune activity of the present invention prevents the proliferation and recurrence of cancer cells and promotes the production of interferons and interleukins that activate immune cell functions, thereby preventing cancer, cell infection or viral infection and treatment. .

Claims (3)

30-50% by weight of chitooligosaccharide, 25-35% by weight of beta glucan, 5-15% by weight of fingerling mushroom fruiting body extract, 2-10% by weight of Ganoderma lucidum fruiting body, 2-10% by weight of vitamin mineral mix, vitamin C 0.5- Immune activity enhancing composition comprising 5% by weight and 0.5-5% by weight of biozymes The method according to claim 1, wherein 42% by weight of the chitooligosaccharide, 29% by weight of beta glucan, 10% by weight of fingerling mushroom fruiting body extract powder, 7% by weight of Ganoderma lucidum fruiting body powder, 7% by weight of vitamin mineral mix, 3% by weight of vitamin C and bio Ximees composition for enhancing immune activity, comprising 2% by weight Composition for enhancing immune activity, characterized in that the composition of claim 1 or 2 is used for the prevention of cancer, bacterial infection or prevention or treatment of viral infection.