KR20010110001A - Cosmetics composition containing Psoralea corylifolia L. extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition, characterized in that it contains hazelnut extract, the cosmetic composition of the present invention due to the lack of female hormones due to the cell growth effect, collagen synthesis effect and hyaluronic acid synthesis effect by hazelnut extract It can suppress skin aging.

Description

Cosmetic composition containing hazelnut extract {Cosmetics composition containing Psoralea corylifolia L. extracts}

The present invention relates to a cosmetic composition containing a substance extracted from the fruit or seed of the hazelnut (Phaseolus vulgaris L.) (hereinafter also referred to collectively as "hazelnut extract").

In the field of cosmetics, the most important problem is to reduce wrinkle formation or to improve wrinkles among the problems to be overcome in order to suppress skin aging.

Unlike the skin of men, women's skin is characteristically elastic, soft and moist, sebum secretion and hairless because of the effect of female hormones. The physiological effects of the female hormone estrogen on the skin suggest that it stimulates fibroblasts in the dermis to promote collagen synthesis and collagen metabolism and is very important for skin moisture retention. Hyaluronic acid) Promotes synthesis, giving skin elasticity, softness, flexibility and moisturizing properties. It increases the proliferation effect of basal layer cells in the epidermis, making the epidermis thicker to strengthen skin tissue. And by controlling the sebaceous gland (sebaceous gland) to suppress the growth of sebaceous gland to reduce the production of sebum (sebum), to lower the hair growth rate to keep the hair thin and short. In particular, it is involved in collagen metabolism, inhibits over-glycation of collagen, induces post-translational modification, and induces collagenase, a collagen degrading enzyme. Regulates expression to inhibit degradation of collagen. As such, the main function of female hormone on skin is to strengthen skin activity and make skin soft, elastic and supple.

However, as the reproductive organs, the secretory sites of female hormones, age, the function gradually deteriorates, and the production of female hormones is stopped before and after menopause. In organs that maintain fertility, hormonal balance is maintained by ovarian estrogen production, but during menopause, the production of female hormones stops, and the balance of hormones is destroyed. Endocrine ageing caused by female hormone deficiency is promoted. Endocrine aging is a type of skin aging that is physiological skin aging that occurs during menopause.

Female hormone deficiency causes skin changes, a typical phenomenon of skin dryness. The lack of female hormone in the fibroblasts inhibits the production of collagen, elastic fibers (elastic fiber, etc.), the skin becomes thinner, the elasticity of the skin is reduced. It is also a synthetic place of hyaluronic acid, which weakens the skin's water retention function. And collagen glycation (collagen glycation) is increased to increase the cross-linking of collagen, the skin is hardened (Sclerosis) is reduced elasticity.

The results of research on a solution for suppressing skin aging due to the deficiency of female hormone have been reported. Hormone Replacement Therapy is a typical supply of scarce female hormones. In the case of supplying female hormones, there are prominent effects such as increased collagen synthesis, hyaluronic acid synthesis, and epidermal cell proliferation, resulting in increased epidermal thickness. However, cosmetics do not use female hormones directly, so other methods have been taken. Cosmetic considerations are not indirect treatment of skin hormonal deficiency by treating moisturizers, aging inhibitors, free radical scavengers and sunscreens, but indirect treatments using general inhibitors of skin aging.

In order to improve women's skin rapidly progressing before and after menopause, it is important to develop a substance having a similar function with female hormones. From the cosmetic point of view to suppress skin aging, female hormone-like substance is a substance having collagen synthesis and hyaluronic acid synthesis promoting effect.

Therefore, an object of the present invention is to provide a new cosmetic composition having collagen synthesis and hyaluronic acid synthesis enhancing effect.

The inventors of the present invention found that the hazelnut extract was very excellent in cell proliferation effect, collagen synthesis effect and hyaluronic acid synthesis effect during the exploratory research on the herbal medicines native to Korea to develop female hormone-like substances from plants. Will be completed.

Therefore, according to this invention, the cosmetic composition containing a hazelnut extract is provided.

Moreover, according to this invention, the cosmetic composition containing 0.05-10.0 weight% of hazelnut extract on the dry weight basis of the whole composition is provided.

Hereinafter, the present invention will be described in more detail by way of preferred embodiment.

Hazelnut is annual plant 40-90cm high, with egg-shaped leaves alternately attached and yellow purple flowers gather like ears. The fruit is elliptical with one seed in the inside. It is native to the tropics and is grown as a herb in Korea.

Hazelnut extract added to the cosmetic composition of the present invention can be prepared, for example, in the following manner.

First, the fruit or seeds of the hazelnuts are dried, washed with purified water and then dried to be powdered, and the extraction solvent is added in an amount of 1 to 15 times the dry weight of the powdered hazelnut fruit or seeds, and then at 1 to room temperature. After soaking for 15 days to extract the active ingredient, the extract solution can be concentrated under reduced pressure to obtain the desired hazelnut seed extract. Although not particularly limited, extraction solvents include water, methanol, ethanol, acetone, ethyl acetate, 1,3-butylene glycol, hexane, diethyl ether, and normal propanol (n). -propanol), iso-propanol, and one or two or more mixtures selected from the group consisting of n-butanol are preferred. Particularly preferred extractant is one, or a mixture of two or more selected from the group consisting of water, methanol, ethanol and 1,3-butylene glycol. Although not particularly limited, a distillation apparatus equipped with a cooling condenser may be used for the vacuum concentration of the extraction solution.

Hazelnut extract contained in the composition has a function of inhibiting skin aging by expressing a cell proliferation effect, collagen synthesis effect and hyaluronic acid synthesis effect when applied to the human body.

The cosmetic composition of the present invention preferably contains the hazelnut extract in an amount of 0.05 to 10.0% by weight based on the dry weight of the total composition.

Preferably, the product form of the cosmetic composition to which the present invention can be applied includes, for example, cosmetics for basic products such as lotion, cream, essence, cleansing foam, cleansing water, pack, body oil, etc .; Color cosmetics such as foundation, lipstick, mascara, makeup base, etc .; Hair cosmetics such as shampoo, conditioner, hair conditioner, hair gel, and the like.

Features and other advantages of the present invention as described above will become more apparent from the following examples.

Examples 1 to 21

1. Preparation of Hazelnut Extract

Example 1

Hazelnut fruit was washed with purified water, dried and ground to prepare hazelnut powder. 1 kg of hazelnut powder was added to 5 L of water, extracted for 3 to 5 days at 15 to 40 ° C., filtered through a 300 mesh filter cloth, left at 4 to 15 ° C. for 7 to 10 days, and then aged at low temperature. It was. The filtrate was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser and then dried to obtain a hazelnut extract. Three times the average extraction amount was 110.34g (dry weight), the yield was 11.0 ± 098%.

Example 2

1 kg of hazelnut powder prepared in the same manner as in Example 1 was added to 5 l of 10% ethanol, extracted in an extractor equipped with a cooling condenser for 3 days, filtered through a 300 mesh filter cloth, and left at 4 to 15 ° C. for 7 to 10 days to be aged at low temperature. It was then filtered through Whatman twice filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser and dried to obtain a hazelnut extract. Three times the average extraction amount was 105.4g (dry weight), the yield was 10.5 ± 0.8%.

[Examples 3 to 21]

To extract the hazelnut extract in the same extraction yield as described in Table 1 using the solvent described in Table 1 below.

division Solvent Extraction yield (% mean ± standard deviation) Example 3 20% ethanol 13.3 ± 0.7 Example 4 30% ethanol 12.4 ± 0.9 Example 5 40% ethanol 10.1 ± 0.8 Example 6 50% ethanol 9.4 ± 1.0 Example 7 60% ethanol 8.7 ± 0.7 Example 8 70% ethanol 6.6 ± 0.6 Example 9 80% ethanol 5.3 ± 0.8 Example 10 90% ethanol 3.7 ± 0.5 Example 11 100% ethanol 2.5 ± 0.3 Example 12 80% methanol 6.2 ± 0.5 Example 13 100% methanol 3.1 ± 0.5 Example 14 Acetone 1.4 ± 0.2 Example 15 Ethyl acetate 1.9 ± 0.2 Example 16 50% 1,3-butylene glycol aqueous solution 6.9 ± 0.7 Example 17 Hexane 0.9 ± 0.1 Example 18 Diethyl ether 0.5 ± 0.1 Example 19 Normal-propanol 0.3 ± 0.1 Example 20 Iso-propanol 0.4 ± 0.1 Example 21 Normal-butanol 0.2 ± 0.1

2. Cell Proliferation Effect of Hazelnut Extract

1) Experiment Method

Human normal fibroblasts are inoculated to 1x104 cells in each well of a 96-well microplate and incubated in DMEM medium for 24 hours. After incubation, the hazelnut extract prepared in Examples 1 to 21 was replaced with a serum-free DMEM medium adjusted to a final concentration of 250 μg / ml, followed by further incubation for 24 hours. Add 10 µl of MTT solution [3- (4,5-Dimethyl-thiazole-2-yl) 2,5-diphenyl tetrazolium bromide: 5 mg / ml], and leave for 4 hours, and then discard the media. 100 μl of dimethyl sulfoxide (DMSO: dimethyl sulfoxide) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm with a microplate reader.

2) Experiment result

The cell proliferation effect of hazelnut extract was calculated by the following formula and the results are shown in Table 2.

extract Cell proliferation effect (%) Example 1 13.4 ± 1.5 Example 2 17.3 ± 1.6 Example 3 18.1 ± 2.4 Example 4 22.6 ± 1.1 Example 5 30.7 ± 1.6 Example 6 36.5 ± 1.1 Example 7 41.3 ± 1.8 Example 8 49.8 ± 2.0 Example 9 43.2 ± 2.1 Example 10 49.3 ± 2.4 Example 11 43.8 ± 1.5 Example 12 44.9 ± 1.9 Example 13 38.6 ± 1.5 Example 14 24.3 ± 1.6 Example 15 37.4 ± 1.4 Example 16 52.1 ± 2.1 Example 17 8.8 ± 0.3 Example 18 9.6 ± 0.9 Example 19 16.7 ± 1.3 Example 20 19.3 ± 1.7 Example 21 14.3 ± 1.2

* The above value is the average of 3 runs

3. Experimental Study of Collagen Synthesis Enhancement Effect of Hazelnut Extract

1) Experiment Method

Human Normal fibroblasts are seeded in 96-well microplates (2 × 10 4 cells / well) and incubated for 24 hours. After incubation the medium is replaced with DMEM without serum. At this time, put the hazelnut extract of various concentrations with the medium and incubated for 48 hours. Hazelnut extract was prepared in Example 8. Collagen synthesis is promoted by adding 50 μg / ml of ascorbic acid before the last 24 hours of culture. After incubation, each well is washed, replaced with DM medium containing no serum, and further incubated for 24 hours. After incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was converted into PICP amount by the amount of collagen newly synthesized using Kit (Takara, Kyoto, Japan). As measured by, the amount of PICP was converted into ng / 2 × 10 4 cells.

2) Experiment result

Hazelnut extract showed a synthetic effect of collagen I in human normal fibroblasts. The experimental results are shown in Table 3.

Hazelnut Extract Concentration Collagen Synthesis Amount (ng / 2x104) 0 (control) 151 100 μg / ml 183 250 μg / ml 224 500 μg / ml 254

3. Experiment of Cell Proliferation Effect of Hazelnut Extract

1) Experiment Method

The experiment was carried out in the same manner as in Experiment 1. Hazelnut extract was prepared in Experimental Example 8.

2) Experiment result

Hazelnut extract showed a pronounced cell proliferation effect in human normal fibroblasts and the results are shown in Table 4.

Hazelnut Extract Concentration Cell proliferation effect (%) 0 (control) - 100 μg / ml 23.4 250 μg / ml 39.2 500 μg / ml 72.1

5. Experimental Effect of Hyaluronic Acid Synthesis of Hazelnut Extract

1) Experiment Method

Human Normal fibroblasts are seeded in 6-well culture plates (3 × 10 4 cells / well) and incubated for 48 hours. In this case, DM medium containing 10% FCS (Fetal calf serum), penicillin, streptomycin (100 µg / ml) and the like was used. After incubation, the medium is replaced with medium containing hazelnut extract of various concentrations and incubated for 24 hours. Hazelnut extract was prepared in Example 8. After incubation, only the media portion is collected and trichloroacetic aicd is added to a final concentration of 10% in order to remove proteins and stored at 4 ° C for 24 hours. Centrifugation of this solution to dialysis (dialysis) of the supernatant, and treated with NaCl to a final concentration of 0.04M and then to the cetylpyridium chloride (cetylpyridium chloride) is added to precipitate hyaluronic acid. The precipitate is dissolved in 4M NaCl by centrifugation, reprecipitated in ethanol and the precipitate is dissolved in purified water. To quantify hyaluronic acid, electrophoresis is performed using a Cellulose acetate strip. Hata, R. & Nagai, Y. (1972) Anal. Biochem. 45, 462-468. After electrophoresis, immersed in 3% acetic acid containing 0.5% Alcian blue and washed. Dry and measure the amount with a 600 nm scanner.

2) Experiment result

Hazelnut extract showed a hyaluronic acid synthesis-promoting effect in human normal fibroblasts, and the results are shown in Table 5.

Hazelnut Extract Concentration Hyaluronic Acid Synthesis Enhancement Effect 100 µg / ml 27.8 500 µg / ml 41.7

6. Prescription of cosmetic composition containing hazelnut extract

Various cosmetic compositions were prepared by the prescription as shown in Tables 6-11. Table 6 is a soft cosmetics, Table 7 is a milk lotion, Table 8 is a nutrition cream, Table 9 is an essence, Table 10 is a massage cream, Table 11 shows a prescription of the pack.

Flexible lotion ingredient Content (% by weight) Hazelnut extract 5.0 1,3-butylene glycol 6.0 Sodium hyaluronate 2.0 glycerin 4.0 Polyethylene Glycol (PEG) 4000 1.0 Polysorbate 20 0.5 ethanol 10.0 antiseptic Quantity Benzophenone-9 0.05 incense Quantity Purified water Remaining amount Sum 100

Milk Croissant ingredient Content (% by weight) Hazelnut extract 5.0 Stearic acid 0.4 1,3-butylene glycol 6.0 Cetostearyl alcohol 1.2 glycerin 4.0 Glyceryl Stearate 1.0 Triethanolamine 0.25 Tocopheryl acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.6 Carboxyvinyl Polymer 0.15 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Nutrition cream ingredient Content (wt%) Hazelnut extract 5.0 vaseline 7.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 2.0 Stearic acid 1.5 Floating paraffin 10.0 Beeswax 2.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.8 Squalane 3.0 1,3-butylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Tocopheryl acetate 0.1 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

essence ingredient Content (% by weight) Hazelnut extract 5.0 glycerin 10.0 PEG 1500 2.0 Allantoin 0.1 Panthenol 0.3 EDTA 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyldodeceth-25 0.6 ethanol 6.0 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

Massage cream ingredient Content (% by weight) Hazelnut extract 3.0 Glyceryl Stearate 2.0 Cetostearyl alcohol 2.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 Squalene 5.0 Mineral oil 35.0 Dimethicone 1.0 Xanthan Gum 0.1 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanol amine 0.5 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

pack ingredient Content (% by weight) Hazelnut extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 Fiji 1500 2.0 Panthenol 0.4 Allantoin 0.1 ethanol 6.0 Fiji 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

As described above, the cosmetic composition containing hazelnut extract according to the present invention provides the effect of inhibiting skin aging due to the deficiency of female hormones due to the cell proliferation effect, collagen synthesis effect and hyaluronic acid synthesis effect by hazelnut extract It is a high value-added novel and useful invention.

Claims (4)

Cosmetic composition containing hazel fruit or seed extract. The cosmetic composition according to claim 1, wherein the hazelnut extract is contained in an amount of 0.05 to 10.0% by weight based on the total dry weight of the composition. The method of claim 1, wherein the hazelnut extract is dried fruit or seeds of the hazelnuts, washed with purified water and then dried to powder, and the extraction solvent 1 to 15 with respect to the dry weight of the powdered hazelnut fruit or seeds A cosmetic composition, characterized in that the extract is concentrated under reduced pressure after adding in an amount of pear and immersing at room temperature for 1 to 15 days to extract the active ingredient. The method of claim 3, wherein the extractant is selected from the group consisting of water, methanol, ethanol, acetone, ethyl acetate, 1,3-butylene glycol, hexane, diethyl ether, normal propanol, iso-propanol and normal-butanol. Cosmetic composition, characterized in that one or a mixture of two or more.