KR19980026946A - Granules for Control of Pine Needle Lump Fly Containing Beauveria sp. - Google Patents

Abstract

The present invention is to use the Beauveria sp. Fungus for the control of pine needles flies, new use as a control of pine needles flies of the genus Boberia, the preparation for pine needles control to contain the fungus of the genus Boberia and It relates to a manufacturing method thereof. Granules for controlling pine needles fly control of the present invention is composed of mold spores, surfactants, nutrients, disintegrants, etc., showing excellent pine needles fly control effect in the natural environment of Korea.

Description

Beauveria sp. Granules for controlling pine needles flies containing fungi and methods for preparing the same.

Figure 1 shows an electron micrograph of the Beauveria bassiana fungus of the present invention.

2 shows an electron micrograph of the Beauveria bassiana fungus of the present invention.

Figure 3 shows an electron micrograph of the Beauveria bassiana fungus of the present invention.

4 shows an electron micrograph of the Beauveria brongniartii fungus of the present invention.

5 shows an electron micrograph of the Beauveria brongniartii fungus of the present invention.

6 shows an electron micrograph of the Beauveria brongniartii fungus of the present invention.

7 shows an electron micrograph of the Beauveria brongniartii fungus of the present invention.

[Objective of the invention]

The present invention is to use the Beauveria sp. Fungus to control pine needles flies, the new use of the fungus of the genus Boberia as a pine needles fly control, the pine needles control of pine needles containing the fungus of the genus Boberia And a method for producing the same.

More specifically, the present invention is to control the pine needles of the present invention containing Beauveria sp. Fungi, which is used for the control of pine needles flies, which are pests that have a lethal effect on pine trees . Solvent is 10-20% water content, 0.2-5mm particle size and consists of mold spores, surfactants, nutrients, disintegrant, etc., and shows excellent pine leaf fly control effect in our natural environment.

[Technical Field to which the Invention belongs and Conventional Technology in the Field]

The pine needles fly flies on the pine needles of the native species of pine (Red pine) and gomsol (sea pine) and sucks the juice of the pine to stop the growth of pine needles.

The pine damage caused by pine needle humps was first discovered in Mokpo and Seoul's Biwon in 1929, and now the damage has been observed nationwide. However, worldwide, it is known that severe damage caused by pine needles flies is limited to Korea and parts of Japan.

The methods of controlling pine needles flies designed so far include: 1) air control of chemical insecticides, 2) spraying chemical insecticides on the surface of the ground, 3) water injection, 4) using natural insect beetles, 5 A) how to use the younger dogs that eat pruning, and 6) how to use pathogenic microorganisms.

However, there are some problems in using these methods to actually control pine needles. First of all, the use of chemical insecticides can also damage useful insects and natural enemies, destroying forest ecosystems and polluting the environment. When using intravenous injection, a large amount of manpower is required, and the drug used is highly toxic to the human body, which may cause poisoning of the control agent.

In order to overcome the above problems, a biological insecticidal method is used. Specifically, in the case of using a natural insect bite, the host parasitic rate is low as 15%, and the area that can be controlled is limited. In addition, there is a difficulty in the case of using the wild horned pecking insects of pine needles or hummingbirds because data on the breeding of wild horned birds are insufficient.

In addition, the method of using the pathogenic microorganism is considered to have a number of useful to date, but the development is still in the early stage.

The present inventors have discovered that the beetle of Beauveria sp. Kills pine needles larvae while searching for the pathogenic microorganisms to control the pine needles flies that cause fatal harm to the forest ecosystem of Korea . In addition, pine needles containing the genus Boberia ginseng were prepared for the control granules to complete the present invention.

[Technical Challenges to Invent]

It is an object of the present invention to provide a novel use of the fungus of the genus Boberia, which uses the genus Beauveria sp .

Another object of the present invention is to provide a fungus of the genus Boberia, which is suitable in the natural environment of Korea among the fungus of the genus Boberia controlling pine needles.

Fungi of the genus Boberia that control pine needles flies include Beauveria bassiana and Beauveria brongniartii . Specifically, the present invention provides a Beauveria bassiana isolated from the wild of our country, and among them, the Boberia Bassiana HY-1 strain having excellent control effect on pine needles flies on March 14, 1995 It was deposited in the Genetic Bank of Korea Institute of Science and Technology.

In addition, an object of the present invention is to provide a granule for controlling pine needles flies containing Beauveria sp . Granules for controlling pine needles fly control of the present invention is composed of mold spores, nutrients, surfactants, etc. In addition, it may include a disintegrant and excipient.

Surfactants used in the granules for controlling pine needles of the present invention include polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl Ether, sodium tripolyphosphate, polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl aryl polymer, polyoxyalkylone alkyl phenyl ether, polyoxyethylene nonyl phenyl ether, sodium sulfonate naphthalene formaldehyde Etc. may be included.

The disintegrant used in the pine needles for controlling the ginseng fly control of the present invention may include bentonite, talc, diamond, kaolin, calcium carbonate and the like.

Granules for controlling pine needles of the present invention is preferably prepared in the composition of the mold spores 30-60% (all of the content is weight ratio), 2-16% surfactant, 5-20% nutrients. In addition, it is more preferable to include 10-30% of disintegrant.

In addition, it is preferable that the granules for controlling pine needles fly control of the present invention is 0.2-5mm in size.

Another object of the present invention is to provide a method for preparing granules for controlling pine needles or flies containing Beauveria sp .

Specifically, the present invention is mixed with crushed fungi and disintegrant of Boberia, and then mixed with a surfactant, nutrients and disintegrant, kneaded to appropriate moisture content, granulated by an extruder and dried to control pine needles fly Prepare a solvent. At this time, it is preferable to knead the above constituent ingredients so as to contain appropriate moisture, and to dry to 60 ℃ or less so that the moisture content is 10-20%.

Composition and Action of the Invention

The present invention is described in detail as follows.

If you look at the life history of pine needles, the adult leaves of pine needles are 1.75 (male)-2mm (female), wing length is 1.75-2.05mm, and body width is 0.8mm. The leaves of pine needles are long oval, 0.19-0.26 X 0.04-0.09mm, and pale yellow. The larvae are 2.06 x 0.73mm in size and pale yellow, and fusiform cords are 5.2-9.5 x 1.6-2.8mm in size. At first they are reddish green but after 1 month, the tissue turns brown.

The pine needle hog flies occur once a year and the larvae overwinter in the ground or in the umbel. Imagoes appear from late May to late June. After mating, 6.3 eggs are laid in one place on a pine tree, then migrated to another leaf to spawn again. After 1-2 days after spawning, the eggs hatch, and the larvae descend to the base of the pine needles, sucking on the juice of the pine and forming a scourge. Usually one lobe contains 5-6 larvae. In late August to early September, the outside of Chungyoung turns brown, and the damaged pine needles discolor from mid-October. After rain or rain, the larvae exit the worms and winterize by about 1–3 cm into the corroded land (Dolomite, Agriculture and Pests, Hyangmunsa, 1978).

So far, the fungus of the genus Boberia has been used as a pesticide against grasshoppers, and has been reported to be safe for humans and livestock. Therefore, it can be useful for controlling pine needles (Tanaka and Kaya, Insect Pathology). , p. 323, Academic Press, 1993; Weiser, Chap. 16, ed. by Kurtak, Microbial and Viral Pesticides, Marcel Dekker, 1982).

The control effect of pine needle nocturnal larvae by the genus Beauveria sp. Is examined through the procedure of Experimental Example 1 below with reference to the pine needle nocturnal life history.

Experimental Example 1

1) How to collect pine needle larvae

Lay a layer of salt-free sand 20 cm and red pine sawdust 10 cm high. Then, in late autumn, the severely damaged pine branches are collected and placed on top of them. It is refrigerated at 4 ° C. and left at room temperature to obtain pine needle larvae.

2) Bovine Formation of Bovine Fungus

The fungus is infected by incubating 20 pine needles larvae and fungi of Boberia for 3 weeks on a glass dish measuring 8.5cm in diameter with a filter paper moistened with water (Watman No. 1). After the infected larva develops and dies, the fungus is observed to cover it white.

3) Outdoor test method

In February, stack a layer of sand 5 cm and sawdust 5 cm in a box measuring 56 x 36 x 12 cm, and then inoculate 1,000 pine needle larvae on it. This box is buried in the soil in the Daeduk Research Complex, and the fungi cultured thereon are dispersed in 500 ml of 10 ¹⁵ spores. After raining in April, the box is pulled out to manually isolate the pine needles larvae.

By investigating by the method of Experimental Example 1 above, it can be observed that the fungus of the genus Boberia infects the pine needles larvae in the laboratory as well as outdoors. Also, in a beam Beria collected in our country, the natural environment fungi as well as being quoted Bo Beria in fungal strains Bo Beria Bridge Ana (Beauveria sp.) (Accession number: KCTC 6297 and KCTC 6298) and Bo Beria probe rongna Artie (accession No .: KCTC 6299) also have a control effect on pine needles flies. Therefore, it can be seen that the fungus of the genus Boberia can be widely used for controlling pine needles.

The present inventors found that the strains of Boberry genus through Experimental Example 1 and Experimental Example 2 can be usefully used to control pine needles fly flies using the Boberry strains isolated from soil of our country We wanted to develop. The fungus of the genus Boberia, which is used for the control of pine needles flies, is sprayed on the natural environment of our country and should be suitable for the soil of our country. Therefore, it is preferable to use the strains of the genus Boberia isolated directly from the soil of our country.

Therefore, in order to obtain a fungus of the genus Boberia suitable for the soil of Korea and excellent control effect on pine needles flies, mold was isolated according to the following Experimental Example 2.

Experimental Example 2

1) Isolation of fungi of the genus Boberia

In order to isolate the fungus of the genus Boberia, which is suitable for use in the natural environment of Korea and to control pine needles flies, the pine needles larvae, pine cone moths infected by the fungus from the forest soil of pine needles fly damage areas around the country And warts are collected. The fungus is placed on a 1.5% potato-glucose solid medium (manufactured by Diffco Co.), which is incubated at 30 ° C. for 2 weeks, and the fungus is separated from each other.

The isolated fungus was incubated at 20 ° C. using oatmeal medium according to the method of de Hoog (de Hoog, Studies in Mycol., 1, 1972, September), and the formation ability of condyne spores formed at this time was compared. Each strain is identified.

As a result, five strains were isolated, one strain was identified as Beauveria bassiana (see FIGS. 1-3), and the other four strains were identified as Beauveria brongniartii (Figure 4). -7). Electron micrographs of these fungal spores are shown in FIGS. 1 to 7.

The fungus of the genus Boberia isolated from Experimental Example 2 exhibiting the best control effect was identified as Boveria bassiana and named this strain as Boberia bassiana HY-1 strain. He was deposited with the Korea Institute of Science and Technology Gene Bank of Biotechnology Research Institute (Accession Number: KCTC 0155 BP).

In addition, in order to know the control effect of pine needles of the genus Boberia on pine needles fly, it is investigated in accordance with the method of Experimental Example 3.

Experimental Example 3

1) Cell Formation Capacity of Boberia Strains

20 pine needle larvae prepared in Experimental Example 2 are transferred to a glass dish of 8.5 cm in diameter coated with filter paper (Watman No. 1) soaked in water.

To 5 g of Boberia Bassiana HY-1 strain (Accession No .: KCTC 0155 BP) isolated in Experimental Example 2 (500 g of wheat bran in 500 ml of water, 3 g of potato glucose broth, 5 g of brain heart infusion, 1 g of manganese sulfate) Inoculate and incubate at 30 ° C. for 6 days. Then, 15 ml of sterile water is added and 1 ml of the suspension obtained by suspending with the vortex is evenly spread on the glass plate containing the pine needle larvae. As a result, after three weeks, all the larvae are surrounded by mold and die, and white spores and hyphae are formed around the carcass.

Further, in addition to the Boberia bassiana HY-1 strain deposited in the present invention, Boberia Bassiana strains (Accession No .: KCTC 6297 and KCTC 6298) already disclosed in the comparative group, Boberia Bronia isolated in Experimental Example 2 Tea ( Beauveria brongniartii ) and the disclosed strain Boberia bronniati (Accession No .: KCTC 6299) are each treated in the same manner as above. As a result, it is confirmed that the various fungi of the genus Boberia show the formation of worms for pine needles flies as shown above.

2) Mortality of Pine Needle Lump Larva Outdoors

A 56 cm x 36 cm x 12 cm box was laminated with a layer of sand 5 cm and sawdust 5 cm high and inoculated with 1,000 pine needles larvae. Next, excavate the soil in Daeduk Research Complex, bury 6 boxes in February, and disperse the above Boberia Bassiana HY-1 (Accession No .: KCTC 0155 BP) strain on the box with 500 spores to 10 ¹⁵ . After the rain in April, the box is taken out and the larvae are manually removed. As a result, the mortality of the pine needles larvae by the fungal infection of the present invention was 45% on average.

In addition, as a result of treatment with various fungal strains in the same manner as above, the mortality rate of the pine needles larvae due to fungal infection was about 40-50%.

The present inventors have concentrated on the preparation of a preparation for controlling deadly pine needles flies that are deadly in the forests of our country, and found that the fungus of the genus Boberia has a control effect on the pine needles flies. After separating the fungus of the genus Boberia to develop a granule for controlling pine needles fly containing the same to complete the present invention.

The fungus of the genus Boberia used in the pine needles for controlling the control of the pine needles of the present invention is a strain having a high viability that can grow even in cold or dry climate, it is suitable for use as a control agent such as pine needles in the form of particles. In particular, the climate of our country, March-April, which is a good time to control pine needles flies, is very dry, so it is advisable to use a fungus of the genus Boberia that survives and can infect pine needles larvae. In addition, the present invention uses the Boberia Bassiana HY-1 strain isolated from the soil of our country in order to manufacture the pine needles for controlling the pine needles suitable for the natural environment of Korea, pine needles fly control effect is more excellent.

Granules for controlling pine needles of the present invention is composed of the fungus spores of the genus Boberia and necessary nutrients, surfactants and moisture required to survive the fungus in the granules, and may further include disintegrants and excipients.

In addition, the pine needles for controlling the ginseng fly control of the present invention includes about 30-60% (both content ratio is weight ratio) of the fungus spores of the genus Boberia, more preferably contains about 50%.

In addition, the pine needles for controlling the ginseng fly control of the present invention includes a surfactant that acts to disperse and disperse the granules of the present invention in addition to the mold spores. Surfactants used in the granules for controlling pine needles of the present invention include polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl Ether, sodium tripolyphosphate, polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl aryl polymer, polyoxyalkylone alkyl phenyl ether, polyoxyethylene nonyl phenyl ether, sodium sulfonate naphthalene formaldehyde Etc. may be included.

In addition, the pine needles for controlling the ginseng fly control granules of the present invention includes the nutrients necessary to grow spores spores when disintegrated by the absorption of water in the natural environment, the nutrients may include starch, dextrin, glucose and the like.

In addition, the granules for controlling pine needles of the present invention includes a disintegrant in order to disintegrate by absorbing moisture from the outside to disperse fungal spores, including swellable bentonite, talc, and kaolin. , Dialite, calcium carbonate and the like. As a result, the granules for controlling pine needles fly control of the present invention exhibits a property that can be disintegrated by a small amount of water, such as dew even when sprayed in a natural environment of a dry climate.

Specifically, the granules for controlling pine needles or fly flies of the present invention are preferably prepared with a composition of 30 to 60% of mold spores, 2 to 16% of surfactants, and 5 to 20% of nutrients. At this time, it is preferable that 10-30% of disintegrant is included. In addition, the particle size is 0.2-5mm, the moisture content is preferably about 10-20%, more preferably 15% of the moisture content. The granules for controlling pine needles of the present invention having such a composition have a characteristic of controlling pine needles of the larvae by dispersing the fungus of the genus Boberia to disintegrate even in the spring dry climate of Korea where the relative humidity is 40% or less.

The present invention, in order to prepare a pine needles control agent containing Beauveria sp. Fungus, to prepare a control agent, Boberia fungus mixed with a disintegrant and then using Jet-O-Mizer (manufactured by Aljet) The mixture is pulverized and mixed with a surfactant, nutrients, disintegrant, etc., and kneaded so that the moisture content is appropriate. The dough is granulated using an extruder, dried at below 60 ° C. until the moisture content is about 10-20% and then passed through the mesh.

At this time, the low moisture content of the pine needles for controlling the pine needles fly control of the present invention, the strains of the genus Boberia should not be killed by the high temperature when the survival rate in the granules or when drying the granules. Therefore, the granules of the present invention are preferably dried at 60 ° C. or lower, and more preferably dried at around 50 ° C. The survival rate of mold spores at 50 ° C is usually about 50%.

In order to confirm the stability of the pine needles for controlling the control granules, the results of the preservation test for the granules of the present invention was confirmed that the survival rate of the mold is reduced to about 30% when left for 8 hours at 70 ℃.

Hereinafter, the present invention will be described in detail by way of examples.

The following examples illustrate the present invention in detail, and the content of the present invention is not limited by the examples.

EXAMPLE 1

Preparation of granules for pine needle control

To prepare granules for controlling pine needles flies, Boberia Bassiana (Accession No .: KCTC 0155 BP) spores obtained by culturing in a bran solid medium (80% bran, 20% soybean meal, 30-60% moisture content) spores 3.3 X 10 ⁹ , bentonite 20% was mixed and then ground using a Jet-O-Mizer (manufactured by Aljet). Here again, sodium tripolyphosphate (sodium tripolyphosphate, thickening) 3%, polycarboxylate (polycarboxylate, thickening) 3%, talc (talc) 14% was mixed and kneaded to a water content of 40%. This was granulated using an extruder (manufactured by Fuji Powder Co., Ltd.) at a diameter of 1.2 mm and a length of 0.5-5 mm, dried at 50 ° C. until moisture content was about 15%, and then passed through 16-30 mesh. At this time, the survival rate of the strain of the present invention was about 50%.

In addition, in order to confirm the stability of the pine needles fly control granules prepared above, the preservation test for the granules of the present invention was carried out. As a result, when the granules were left for 8 hours at 70 ℃, the survival rate of the mold was about 30%.

EXAMPLE 2

Preparation of Pine Needle Control Agents for Controlling Relative Humidity 40%

In order to prepare pine needles for controlling pine needles that can disintegrate when the relative humidity is 40%, the following method was used.

As mentioned above, the size of pine needles for controlling pine needles, especially Boberia Bassiana (Accession No .: KCTC 0155 BP), has a size of 0.2-5.0mm and a water content of 15% (all content ratios in weight). It was appropriate.

In addition, as shown in Example 1, pine needle hump control granules were 50% mold spores, polycarboxylate 3%, sodium lignosulfonate 3%, sodium dialkyl sulfosuccinate (sodium dialkyl sulfosuccinate) 1%, glucose 10%, bentonite (bentonite) 20%, calcium carbonate (calcium carbonate) 13% was prepared.

The above composition was dried at about 50 ° C. for controlling the granules, and when the external relative humidity was 40%, the granules disintegrated by absorbing moisture, thereby discharging the mold spores as an active ingredient. It was able to adapt and settle down.

EXAMPLE 3

In order to prepare a pine needles for controlling the control of the pine needles of another composition showing the same effect as in Example 2, the following method was used.

Pine needles for the control of gypsophila fly Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Sodium alkyl aryl sulfonate 5%, Polyoxyethylene alkyl phenyl ether 1 %, Sodium Dialkyl Sulfosuccinate 1%, Starch 10%, Talc 30%, Dialite 3%.

EXAMPLE 4

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for controlling the control of the fly, Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polycarboxylate 3%, Polyoxyethylene alkyl phenyl ether 1%, Calcium lignosulfonate 3%, Starch 10% Prepared to consist of 20% bentonite, 13% talc.

[Example 5]

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles were used for controlling the control of Bogbaria bassiana (Accession No .: KCTC 0155 BP) 50% spores, 5% polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether and polyoxy Ethylene alkyl aryl polymer (polyoxyethylene alkyl aryl ether and polyoxyethylene alkyl aryl polymer) 3%, glucose 10%, bentonite 20%, was prepared to be composed of 12% talc.

EXAMPLE 6

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for control of gypsophila fly Boberia Bassiana (Accession No .: KCTC 0155 BP) with 50% spores, 1% polyoxyethylene alkyl phenyl ether, 3% sodium tripolyphosphate, 10% starch, 20% bentonite, 16% talc It was made to make up.

EXAMPLE 7

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for controlling the control of the fly, Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polycarboxylate 3%, Polyoxyethylene alkyl aryl ether and Polyoxyethylene alkyl aryl polymer 5%, Starch 10%, Bentonite Prepared to consist of 20% kaolin 12%.

EXAMPLE 8

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for controlling the control of the fly, Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polycarboxylate 3%, Sodium alkyl aryl sulfonate 5%, Sodium dialkyl sulfosuccinate 1%, Starch 10% Prepared to consist of 20% dialite, 11% calcium carbonate.

EXAMPLE 9

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for controlling the control of the leaves were prepared by Boberia Bassiana (Accession No .: KCTC 0155 BP) 50% spores, 3% sodium tripolyphosphate, 5% polyoxyethylene alkyl aryl phosphoric esters, 10% starch, 10% dextrin Prepared to consist of 10% bentonite, 12% diamond.

EXAMPLE 10

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for the control of hummingbird fly, Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polyoxyethylene alkyl aryl polymer special 5%, Calcium lignosulfonate 5%, Polycarboxylate 3%, Dextrin 5% Prepared to consist of 20% bentonite, 12% talc.

EXAMPLE 11

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for the control of the ginseng fly Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polyoxyalkylone alkyl phenyl ether 3%, Sodium tripolyphosphate, Sodium dialkyl sulfostone It was prepared to consist of 1% of sinate, 5% of dextrin, 10% of starch, and 28% of calcium carbonate.

EXAMPLE 12

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for the control of hummingbird fly Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Sodium lignosulfonate 3%, Polyoxyethylene alkyl aryl ether polymer 3%, Glucose 10%, Kaolin 20%, Talc Prepared to consist of 11%.

EXAMPLE 13

The following method was used to prepare granules for pine needle hump control of another composition having the same effect as in Example 2.

Pine needles for the control of the ginseng flies, Boberia Bassiana (Accession No .: KCTC 0155 BP) Spore 50%, Polyoxyethylene nonyl phenyl ether 3%, Sodium sulfonate naphthalene formaldehyde formaldehyde) 2%, sodium dialkyl sulfosuccinate 1%, dextrin 5%, kaolin 20%, diamondite 19% was prepared.

[Effects of the Invention]

The pine needles for control of pine needles humps ( Beauveria sp. ) Fungi of the present invention can be disintegrated by absorbing only dew in a dry climate, so the dry climate of our country, which is the time to control pine needles flies Suitable for use in the present invention, the Boberia Bassiana HY-1 (Accession No .: KCTC 0155 BP) strain used in the granulation of the present invention is directly isolated from the soil of our country, so it is very easy to use in the natural environment of our country.

In addition, since the granules of the present invention are in the form of particles, they are locally administered to pine needles infected with pine needles so that they stably control the pine needles larvae, and thus, the pine needles can be continuously controlled by a small number of times. It is an excellent control agent.

Claims (14)

Beauveria sp. Fungus with pine needle control effect. The Beauveria sp. Fungus (Accession Number: KCTC 0155 BP) according to claim 1, characterized in that it is Beauveria bassiana HY-1. The pine needles fly control agent containing the fungus of the genus Boberia of Claim 1. The granules for controlling pine needles flies according to claim 3, which are composed of mold spores, surfactants, and nutrients. The granules for controlling pine needles flies according to claim 4, further comprising a disintegrant. The granules for controlling pine needles flies according to claim 5, wherein the disintegrants include bentonite, talc, kaolin, diamond and / or calcium carbonate. The granules for controlling pine needles flies according to claim 3, which contain 30 to 60% of fungal spores. 4. The surfactant of claim 3 wherein the surfactant is selected from polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, Polyoxyethylene alkyl aryl phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers and / or sodium sulfonate naphthalene formaldehyde Pine needle humpback control characterized in that the granules. The pine needles fly control granules according to claim 3, wherein the nutrients include starch, dextrin and / or glucose. The granules for controlling pine needles flies according to claim 3, wherein the particle size is 0.2-5 mm. The pine needle hump control granules according to claim 3, wherein the moisture content is 10-20%. The pine needles control agent preparation according to claim 3, comprising 50% mold spores, 2-16% surfactant, 5-20% nutrient, 10-30% swellable bentonite, and 10-30% talc. Preparation of pine needles for controlling pine needles fly control of the fungus and the disintegrant of claim 1, and pulverizing, and then mixing the surfactant, nutrients and disintegrant, kneading to the appropriate moisture content, granulated by an extruder Way. The method for producing granules for controlling pine needles flies according to claim 13, which is dried at 60 ° C or lower.