KR102739134B1 - Methods of obtaining Fucoidan extracts from seaweed and Compositions for moisturizing skin comprising the same - Google Patents

Abstract

The present invention provides a method for producing a fucoidan extract from seaweed, comprising the steps of: fermenting seaweed to obtain a fermented product; obtaining seaweed and a fermented liquid from which seaweed is separated from the fermented product; extracting the seaweed to obtain an extract 1; filtering the fermented liquid from which the seaweed is separated to obtain an extract 2; and mixing the extract 1 and the extract 2 to obtain a fucoidan extract. According to the method of the present invention, an extract including fucoidan can be obtained from seaweed, and a functional composition for promoting skin moisturizing can be provided, which includes the extract thus obtained as an effective ingredient.

Description

Method for obtaining fucoidan extracts from seaweed and compositions for moisturizing skin comprising the same

The present invention relates to a method for obtaining an extract containing fucoidan from domestically produced seaweed and a functional composition for promoting skin moisturizing containing the extract as an effective ingredient.

Recently, the global cosmetics research and development (R&D) market is transforming into a collection of convergence technologies for anti-aging, and bio-cosmetics is moving to the center of the market. In addition, as the boundaries between cosmetics and pharmaceuticals are collapsing, the bio-cosmetics market is expected to develop rapidly.

Bio-cosmetic materials are attracting attention in the cosmetics industry as they have the advantage of ensuring efficacy and stability.

The global cosmetics raw material market size is estimated at 30 trillion won, of which the functional cosmetics raw material market, which extracts only highly effective ingredients from natural products, is worth 6 trillion won, accounting for 20% of the entire functional market.

According to big data analysis by ‘Insight Korea,’ consumers consider moisturizing effects to be the most important factor when using moisturizing cosmetics, and when it comes to key consumption point ingredients, they mainly mentioned plant extracts that do not irritate the skin.

Accordingly, the inventor of the present invention developed an extraction method capable of efficiently extracting polyphenols and marine polysaccharides having high antioxidant activity and moisture activity from domestic seaweed, a plant-based raw material, and extracting them at a high concentration, thereby developing a basic moisture-related cosmetic, and thus reached the present invention.

The present invention provides a method for producing an extract containing fucoidan from seaweed and a functional composition for promoting skin moisturizing containing the fucoidan extract produced thereby as an effective ingredient.

The present invention provides a method for producing a fucoidan extract from seaweed, comprising the steps of: fermenting seaweed to obtain a fermented product; obtaining seaweed and a fermented liquid from which seaweed is separated from the fermented product; extracting the seaweed to obtain an extract 1; filtering the fermented liquid from which the seaweed is separated to obtain an extract 2; and mixing the extract 1 and the extract 2 to obtain a fucoidan extract.

In addition, the present invention provides a functional composition for promoting skin moisturizing, characterized in that it contains a fucoidan extract prepared by the above method as an effective ingredient.

The composition for promoting skin moisturizing according to the present invention can be utilized as a cosmetic composition for preventing skin dryness because it maintains skin moisturizing while maintaining moisture content due to its high moisture content by including fucoidan, which has a skin antioxidant effect and hyaluronidase inhibitory activity.

Figure 1 illustrates a process for producing a fucoidan extract of the present invention.
Figure 2 shows the results of evaluating the antioxidant activity of the soluble and insoluble components of fucoidan extract.
Figure 3 shows the results of evaluating the hyaluronic acid inhibition activity of the soluble and insoluble components of the fucoidan extract.

The present invention provides an extract containing fucoidan, which is a component extracted from seaweed, and a functional composition for promoting skin moisturizing containing the obtained extract.

Specifically, the method for producing an extract containing fucoidan from seaweed of the present invention comprises the steps of fermenting seaweed to obtain a fermented product, as illustrated in FIG. 1; the steps of obtaining seaweed and a fermented liquid from which seaweed is separated from the fermented product; the steps of extracting the seaweed to obtain extract 1; the steps of filtering the fermented liquid from which seaweed is separated to obtain extract 2; and the steps of mixing the extract 1 and the extract 2 to obtain a fucoidan extract.

In the present invention, the seaweed (Undaria pinnatifida) used for extracting the effective ingredient is harvested, dried, and then frozen before use, and then thawed and used.

In addition, in the present invention, the composition is extracted using only water and ethanol instead of organic solvents such as methylene chloride, methanol, and n-butanol, which were mainly used for extracting fucoidan in the past, for use as a cosmetic raw material.

Wakame is desalinated through a desalination process to remove salt, and to efficiently extract fucoidan from the desalinated wakame raw material, a protein-decomposing enzyme (protease) is used to decompose the wakame tissue while not breaking the glycosidic bonds of fucoidan.

Conventional salt removal processes include boiling in water, washing, or leaving in water for a long time. However, such processes may result in the loss of effective ingredients in salt-tolerant plants. Therefore, in the present invention, salting is performed using a powder mixer used for the purpose of mixing, combining, and granulating powders. After distilled water is added to the powder mixer, seaweed to be salted is added and operated for 5 to 10 minutes. As the blades of the powder mixer rotate, the salt in the seaweed dissolves in the distilled water, thereby performing salting. Thereafter, in order to remove moisture in the seaweed that lowers extraction efficiency, a sugar coating machine (ring coating machine) used for the purpose of a process of coating tablets or pills with sugar is used to blow away moisture and dry the seaweed without applying excessive heat.

Dried seaweed and protease are added to sterilized distilled water and fermented while stirring in a microbial fermenter. At this time, dried seaweed: protease are mixed in a ratio of 10:1 to 0.5 and added to sterilized distilled water at a concentration of 10 to 20 g/L. Then, fermented for 20 to 30 hours while stirring at a stirring speed of 20 to 30 rpm. The fermentation temperature is maintained at 35 to 40°C.

After separating the fermented product obtained through the above process, the seaweed is separated, and since fucoidan is in an unstable state in which a compound such as alcohol or phenol is bound to the hydroxyl group of the sugar during the process of decomposing the tissue of the seaweed with protease, it may dissolve in distilled water through the weakened tissue due to the nature of the fucoidan, so the fermented liquid after separating the seaweed is not discarded but concentrated. In addition, the seaweed is re-dried.

The above-mentioned re-dried seaweed is extracted using ethanol, and for smooth extraction, it is extracted while stirring at a temperature of 78℃ or lower, a temperature at which ethanol does not evaporate, for 20 to 24 hours. The extract at this time is called ‘Extract 1’.

The concentrated fermentation liquid without discarding the above is dissolved using a salt solution having a concentration of 50 to 55% to filter out enzymes and decomposition metabolites, and the undissolved substances (salts) are filtered out and discarded. Thereafter, the solution is re-concentrated, dissolved in ethanol, and then the undissolved substances, such as salt components, are filtered out to obtain a solution. The solution thus prepared is called ‘extract 2’.

The above extract 1 and extract 2 are added and concentrated to prepare a fucoidan extract (solid). The prepared fucoidan extract is dissolved in water to obtain a component that is soluble in water (soluble) and a component that is insoluble in water (insoluble). The soluble component is a solution that is dissolved in water, and the insoluble component is obtained as a solid.

The fucoidan extract obtained from the seaweed of the present invention is obtained by dividing into a soluble component soluble in water and an insoluble component insoluble in water, each of which has antioxidant activity and hyaluronic acid inhibition activity, and can be used as a composition for promoting skin moisturizing. In particular, the composition may be a cosmetic composition. In addition, the composition may be provided in the form of an aerosol, a solution, a suspension, a tablet, granules, or powder.

The present invention is explained more specifically through the following examples.

Example

The collected seaweed (Undaria pinnatifida) was dried and stored in a -25℃ freezer, and thawed when used. The composition was extracted using water and ethanol as solvents.

20 g of protease and 20 L of sterilized distilled water were added to 300 g of dried seaweed that had undergone a decontamination process, and stirred (20 rpm, 24 hours) using a microbial fermenter (1.5 ton fermenter, manufacturer: Kobiotech Co., Ltd.). At this time, the temperature was maintained at 40°C.

After the above process, the seaweed was separated from the fermented liquid and re-dried, and the fermented liquid separated from the seaweed was concentrated in a vacuum concentrator at 70°C and -0.07 MPa under reduced pressure.

The above-mentioned re-dried seaweed was placed in ethanol and extracted while stirring at a speed of 200 rpm for 24 hours at a temperature of 78℃ or lower to obtain extract 1.

In addition, the above-mentioned concentrated fermentation liquid was dissolved in 100 mL of a 50% salt solution, and the undissolved substance was filtered using a filter. Afterwards, the solution was re-concentrated by reducing the pressure to -0.07 MPa at 70°C using a vacuum concentrator, dissolved in 50 mL of ethanol, and the undissolved substance was filtered using a filter to obtain the dissolved core, thereby obtaining extract 2.

After mixing the above extracts 1 and 2, the mixture was concentrated under reduced pressure of -0.07 MPa at a temperature of 70°C using a vacuum concentrator to obtain a fucoidan extract (solid), which was dissolved in 50 mL of water to separate the soluble component (the portion dissolved in water) and the insoluble component (the portion not dissolved in water). Afterwards, the soluble portion dissolved in water and the insoluble portion were dried again using a concentrator to make it into a solid state.

evaluation

1. Antioxidant activity evaluation

The obtained soluble and insoluble components were confirmed through DPPH (1,1-diphenyl-2-picrylhydrazyl) reaction.

Prepare a DPPH radical solution by mixing 1.2 mL of DPPH solution prepared by dissolving 2 mg of DPPH (1,1-diphenyl-2-picrylhydrazyl) in 15 mL of ethanol (EtOH) and a solution of 3 mL of EtOH and 0.5 mL of dimethyl sulfoxide (DMSO) in equal ratios. The prepared DPPH radical solution was placed in a cuvette, and the absorbance was measured at a wavelength of 518 nm. The concentration was adjusted so that the measured value was 0.95 to 0.98. To 900 μL of the DPPH radical solution with adjusted concentration, 100 μL of a solution prepared by dissolving the solid-state soluble and insoluble components in ethanol at a concentration of 100 μg/mL was added, and the absorbance was measured at a wavelength of 518 nm after voltexing for 10 minutes.

As a control group, vitamin C, known to have excellent antioxidant activity, aloe, green tea, and rhubarb, a seaweed widely used as cosmetic ingredients, were used (Figure 2).

As a result of the measurement, both the soluble and insoluble components showed antioxidant activity superior to green tea and rhubarb, and the soluble component showed antioxidant activity similar to aloe and vitamin C. This means that it has the ability to protect the skin against active oxygen species received from inside and outside, and it is expected that the fucoidan extract of the present invention can be used as a cosmetic raw material.

2. Evaluation of hyaluronic acid inhibitory activity

Hyaluronic acid, known as an ingredient that provides moisture to the skin and smoothens skin texture, is broken down by hyaluronidase, an enzyme that is synthesized in our bodies. In order to confirm the efficacy of fucoidan extract as a moisturizing cosmetic ingredient, the hyaluronidase inhibitory activity was evaluated.

After adding 100 μL of hyaluronic acid decomposition enzyme solution and 100 μL of sodium phosphate buffer, 50 μL of the soluble component and insoluble component prepared in Evaluation 1 were added. After reacting at 37°C for 10 minutes, 100 μL of hyaluronic acid solution (hyaluronic acid 0.03%, sodium phosphate 300 mM, pH 5.35) was added and reacted at 37°C for 45 minutes. Finally, 1 mL of albuminic acid solution (BSA 0.1%, sodium acetate 24 mM, acetic acid 79 mM, pH 3.75) was added, reacted at room temperature for 10 minutes, and measured at a wavelength of 600 nm. The control group was set the same as the antioxidant experiment (Fig. 3).

As a result of the measurement, the soluble component showed excellent activity, showing values similar to the control group to which vitamin C and hyaluronic acid inhibitor were not added, and from this, it could be expected that the soluble component of seaweed could be used as a raw material for moisturizing cosmetics.

Claims (10)

A step of fermenting seaweed to obtain a fermented product;
A step for obtaining a fermented liquid from which seaweed and seaweed are separated from the above fermented product;
A step of extracting the above seaweed to obtain extract 1;
A step of filtering the fermented liquid from which the seaweed is separated to obtain extract 2; and
A step of mixing the above extract 1 and extract 2 to obtain and concentrate an extract containing fucoidan,
A method for producing an extract containing fucoidan from seaweed, characterized in that in the step of fermenting the seaweed, a protease is used that decomposes seaweed tissue without breaking the glycosidic bond of fucoidan. delete In paragraph 1,
In the step of fermenting the above seaweed,
Wakame: A method for producing an extract containing fucoidan from wakame, characterized by mixing protease in a ratio of 10:1 to 0.5, adding it to sterilized distilled water at a concentration of 10 to 20 g/L, and fermenting it. In paragraph 1,
In the step of extracting the above seaweed to obtain extract 1,
A method for producing an extract containing fucoidan from seaweed, characterized in that the extraction is performed by stirring at a temperature of 78°C or lower for 20 to 24 hours using ethanol as a solvent. In paragraph 1,
In the step of filtering the fermented liquid from which the above seaweed is separated to obtain extract 2,
A method for producing an extract containing fucoidan from seaweed, characterized in that what is filtered from the concentrated fermentation liquid is an enzyme and a decomposition metabolite. In paragraph 1,
In the step of filtering the fermented liquid from which the above seaweed is separated to obtain extract 2,
A method for producing an extract containing fucoidan from seaweed, characterized by dissolving it in a salt solution and ethanol having a concentration of 50 to 55%, and then filtering. In paragraph 1,
A method for producing an extract containing fucoidan from seaweed, characterized in that the extract containing the above fucoidan is divided into a soluble component which is a component that dissolves in water and an insoluble component which is a component that does not dissolve in water. A functional composition for promoting skin moisturizing, comprising an extract containing fucoidan according to any one of claims 1 and 3 to 7 as an effective ingredient. In Article 8,
A composition, characterized in that the composition is a cosmetic composition. In Article 9,
A composition characterized in that the composition is in the form of an aerosol, a solution, a suspension, a tablet, granules or powder.