KR102730776B1 - Antimicrobial composition comprising tap water-based neutral electrolyzed water and lactic acid aqueous solution - Google Patents
Antimicrobial composition comprising tap water-based neutral electrolyzed water and lactic acid aqueous solution Download PDFInfo
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 239000008399 tap water Substances 0.000 title claims abstract description 23
- 235000020679 tap water Nutrition 0.000 title claims abstract description 23
- 239000004310 lactic acid Substances 0.000 title claims abstract description 22
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 22
- 230000007935 neutral effect Effects 0.000 title claims description 15
- 230000000845 anti-microbial effect Effects 0.000 title claims description 11
- 239000007864 aqueous solution Substances 0.000 title description 13
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 42
- 239000000460 chlorine Substances 0.000 claims abstract description 29
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 29
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 229910001220 stainless steel Inorganic materials 0.000 claims description 15
- 239000010935 stainless steel Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 15
- 230000002378 acidificating effect Effects 0.000 abstract description 13
- 238000012545 processing Methods 0.000 abstract description 11
- 239000006227 byproduct Substances 0.000 abstract description 6
- 208000019331 Foodborne disease Diseases 0.000 description 8
- 206010016952 Food poisoning Diseases 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 5
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000007797 corrosion Effects 0.000 description 5
- 238000005260 corrosion Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 241000186779 Listeria monocytogenes Species 0.000 description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
- 239000001974 tryptic soy broth Substances 0.000 description 4
- 108010050327 trypticase-soy broth Proteins 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229940054269 sodium pyruvate Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- GBWARTHIRIVTNI-PJHQGUKWSA-N (2s)-2,6-diaminohexanoic acid;(2r,3s,4r)-2,3,4,5-tetrahydroxypentanal Chemical compound NCCCC[C@H](N)C(O)=O.OC[C@@H](O)[C@H](O)[C@@H](O)C=O GBWARTHIRIVTNI-PJHQGUKWSA-N 0.000 description 1
- 101150082208 DIABLO gene Proteins 0.000 description 1
- 102100033189 Diablo IAP-binding mitochondrial protein Human genes 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000001984 sorbitol MacConkey agar Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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Abstract
본 발명은 수돗물을 수돗물을 전기분해하여 수득한 전해수 및 젖산 수용액을 혼합하여 제조한 항균제 조성물에 관한 것이다. 본 발명의 항균제 조성물은 약산성을 띄어 처리 시 가공 장비를 크게 부식시키지 않는다. 또한, 염소량을 적게 함유하고 있어 소독 부산물 문제 및 잔류 염소 문제를 유발시키지 않는다. 그런데, 본 발명은 약산성 및 낮은 염소의 함유량에도 불구하고 우수한 살균능을 보이는 것으로 확인되었다. The present invention relates to an antibacterial composition prepared by mixing electrolyzed water obtained by electrolyzing tap water and an aqueous lactic acid solution. The antibacterial composition of the present invention is weakly acidic and does not significantly corrode processing equipment during processing. In addition, it contains a small amount of chlorine and thus does not cause problems with disinfection byproducts and residual chlorine. However, it has been confirmed that the present invention exhibits excellent sterilization ability despite its weak acidity and low chlorine content.
Description
본 발명은 '수돗물을 전기분해하여 수득한 전해수' 및 젖산 수용액을 혼합하여 제조한 항균제 조성물에 관한 것으로, 약산성 및 낮은 염소의 함유량에도 불구하고 우수한 항균능을 보인다. The present invention relates to an antibacterial composition prepared by mixing 'electrolyzed water obtained by electrolyzing tap water' and a lactic acid aqueous solution, which exhibits excellent antibacterial activity despite being weakly acidic and having a low chlorine content.
식중독은 세계의 주요 공중 보건 문제 중 하나로 간주되며, 세계보건기구(WHO)는 매년 전 세계적으로 약 6억건의 사고가 발생하고 42만 명의 사망자가 나오는 것으로 추정한다. 이 질병은 식품과 식품 접촉 표면 간의 미생물 교차 오염으로 인해 발생한다. 미국 식품의약국(FDA)에 따르면 식품 가공 시설의 오염된 표면은 식중독 발생에 기여하는 5가지 주요 위험 요소 중 하나이다. Foodborne illness is considered one of the world's major public health problems, with the World Health Organization (WHO) estimating that approximately 600 million incidents occur and 420,000 deaths occur worldwide each year. The illness is caused by microbial cross-contamination between food and food contact surfaces. According to the U.S. Food and Drug Administration (FDA), contaminated surfaces in food processing facilities are one of the five major risk factors contributing to foodborne illness outbreaks.
가공 장비의 식품 접촉 표면에 사용되는 다양한 유형의 재료 중 스테인리스 스틸은 기계적 강도, 내식성, 수명 및 제조 용이성 때문에 가장 널리 사용된다. 하지만, 대표적인 식중독 균인 이콜라이 O157:H7 (Escherichia coli O157:H7), 살모넬라 타이피무리움 (Salmonella typhimurium), 리스테리아 모노사이토제네스 (Listeria monocytogenes)는 스테인리스 스틸 (Stainless steel) 표면에 부착되면 몇 시간 또는 며칠 동안 활성 상태를 유지할 수 있다. 따라서 스테인리스 스틸 표면의 미생물적 안전성을 확보할 수 있는 추가적인 살균공정을 적용해야 할 필요가 있다. Among the various types of materials used for food contact surfaces of processing equipment, stainless steel is the most widely used because of its mechanical strength, corrosion resistance, service life, and ease of manufacturing. However, representative food poisoning bacteria such as Escherichia coli O157:H7, Salmonella typhimurium , and Listeria monocytogenes can remain active for hours or days once attached to the stainless steel surface. Therefore, it is necessary to apply an additional sterilization process to ensure the microbiological safety of the stainless steel surface.
한편, 산성 전해수는 희석 NaCl 용액을 양극에 통과시켜 전기 분해하여 생성되며 2.0 ~ 4.0 pH, 산화 환원 전위 (ORP) 1100 mV, 유효 염소 농도가 10 ~ 90 mg/L인 용액이다. 차아염소산나트륨을 대체할 수 있는 잠재적인 소독제이며 순수한 염소로 소독하는 것보다 세균 세포에 더 심각한 손상을 일으켜 다양한 병원체에 대한 강력한 살균 효과가 있는 것으로 알려져 있다. 그러나, 산성 전해수의 높은 산성도는 장비의 부식을 유발하기 때문에 사용에 제한사항이 있으며, 인체 건강에 부정적인 영향도 미칠 수 있다. 또한, 산성 전해수의 높은 수준의 염소는 트리할로메탄(trihalomethane) 및 할로아세트산(haloacetic acid)과 같은 소독 부산물을 다량 생성하는데, 환경에 악영향을 끼칠 뿐만 아니라, 공정에서 잔류하여 식품 품질에도 영향을 줄 수 있다. 게다가, FDA의 ‘식품 가공 시설에서 소독 후 염소의 잔류량은 3 mg/L를 초과할 수 없으며 초과 시 반드시 세척 추가 공정을 거쳐야 한다.’는 규정이 있는데, 이에 산성 전해수를 사용하는 경우에는 추가적인 세척 공정이 필요하게 된다.Meanwhile, acidic electrolyzed water is generated by electrolyzing a diluted NaCl solution through the anode and has a pH of 2.0 to 4.0, an oxidation-reduction potential (ORP) of 1100 mV, and an effective chlorine concentration of 10 to 90 mg/L. It is a potential disinfectant that can replace sodium hypochlorite and is known to have a strong bactericidal effect on various pathogens by causing more serious damage to bacterial cells than disinfection with pure chlorine. However, the high acidity of acidic electrolyzed water has limitations in its use because it causes corrosion of equipment, and it can also have negative effects on human health. In addition, the high level of chlorine in acidic electrolyzed water generates a large amount of disinfection byproducts such as trihalomethanes and haloacetic acids, which not only have a negative impact on the environment, but can also affect food quality if they remain in the process. In addition, there is a regulation by the FDA that states, ‘The residual amount of chlorine after disinfection in food processing facilities cannot exceed 3 mg/L, and if it exceeds this amount, an additional washing process must be performed.’ Therefore, when using acidic electrolyzed water, an additional washing process is required.
산성 전해수의 경우 강력한 항균 효능을 가지고 있지만, 산성이 강해 사용에 제한사항이 있으며, 높은 염소량으로 인해 소독 부산물 문제 및 잔류 염소 문제를 유발한다. 따라서, 본 발명에서는 낮은 산성도를 가지며, 염소량이 적음에도 불구하고 우수한 살균 효능을 보이는 항균제 조성물을 제공하고자 한다.Acidic electrolyzed water has a strong antibacterial effect, but its use is limited due to its strong acidity, and it causes problems with disinfection by-products and residual chlorine due to its high chlorine content. Therefore, the present invention seeks to provide an antibacterial composition that exhibits excellent sterilization efficacy despite its low acidity and low chlorine content.
본 발명은 수돗물을 전기분해하여 수득한 전해수 및 젖산 수용액을 혼합하여 제조한 것을 특징으로 하는 항균제 조성물을 제공한다.The present invention provides an antibacterial composition characterized by being manufactured by mixing electrolyzed water obtained by electrolyzing tap water and a lactic acid aqueous solution.
본 발명의 항균제 조성물에 있어서, 상기 전해수는 바람직하게 pH 7.52~7.88의 중성 전해수인 것이 좋다.In the antibacterial composition of the present invention, the electrolytic water is preferably neutral electrolytic water having a pH of 7.52 to 7.88.
본 발명의 항균제 조성물에 있어서, 상기 항균제 조성물은 바람직하게 유효 염소를 4.14~5.06 ppm, 젖산을 0.08~0.12 %(v/v) 농도로 포함하는 것이 좋다.In the antimicrobial composition of the present invention, the antimicrobial composition preferably contains 4.14 to 5.06 ppm of effective chlorine and 0.08 to 0.12% (v/v) of lactic acid.
또한, 본 발명은 상기 수돗물을 전기분해하여 수득한 전해수 및 젖산 수용액을 혼합하여 제조한 것을 특징으로 하는 항균제 조성물을 사용하여 스테인리스 스틸(Stainless steel)을 살균하는 것을 특징으로 하는 스테인리스 스틸(Stainless steel)의 살균 방법을 제공한다.In addition, the present invention provides a method for sterilizing stainless steel, characterized in that it sterilizes stainless steel using an antibacterial composition characterized in that it is prepared by mixing electrolyzed water obtained by electrolyzing tap water and a lactic acid aqueous solution.
본 발명의 '수돗물을 전기분해하여 수득한 전해수'와 젖산 수용액을 동시에 함유하는 항균제 조성물은, 약산성을 띄어 처리 시 가공 장비를 크게 부식시키지 않는다. 또한, 염소량을 적게 함유하고 있어 소독 부산물 문제 및 잔류 염소 문제를 유발시키지 않는다. 그런데, 본 발명은 상기와 같은 약산성 및 낮은 염소의 함유량에도 불구하고 우수한 살균능을 보이는 것으로 확인되었다. The antibacterial composition of the present invention, which contains both 'electrolyzed water obtained by electrolyzing tap water' and a lactic acid solution, is weakly acidic and does not significantly corrode processing equipment during processing. In addition, it contains a small amount of chlorine and does not cause problems with disinfection byproducts or residual chlorine. However, it was confirmed that the present invention exhibits excellent sterilizing ability despite the weakly acidic and low chlorine content as described above.
도 1은 수돗물로부터 중성 전해수를 제조하기 위해 사용한, 전해수 생성 장치를 개략적으로 나타낸다.Figure 1 schematically illustrates an electrolytic water generation device used to produce neutral electrolytic water from tap water.
본 발명은 수돗물을 전기분해하여 수득한 전해수 및 젖산 수용액을 혼합하여 제조한 것을 특징으로 하는 항균제 조성물을 제공한다.The present invention provides an antibacterial composition characterized by being manufactured by mixing electrolyzed water obtained by electrolyzing tap water and a lactic acid aqueous solution.
일반 산성 전해수의 경우 강력한 항균 효능을 가지고 있지만, 산성도가 강해 사용에 제한사항이 있으며, 높은 염소량으로 인해 소독 부산물 문제 및 잔류 염소 문제를 유발한다. In the case of general acidic electrolyzed water, it has strong antibacterial effects, but its use is limited due to its strong acidity, and the high chlorine content causes problems with disinfection by-products and residual chlorine.
하지만, 본 발명에서는 '수돗물을 전기분해하여 수득한 전해수'와 젖산 수용액을 혼합 사용함으로, 약산성을 띄고, 낮은 유효 염소 농도를 나타냄에도 불구하고 우수한 항균 효능을 보이는 항균제 조성물을 개발할 수 있었다.However, in the present invention, by mixing and using 'electrolyzed water obtained by electrolyzing tap water' and a lactic acid solution, it was possible to develop an antibacterial composition that exhibits excellent antibacterial efficacy despite being weakly acidic and having a low effective chlorine concentration.
본 발명의 항균제 조성물은 바람직하게 유효 염소를 4.14~5.06 ppm, 젖산을 0.08~0.12 %(v/v) 농도로 포함하는 것이 좋다. 유효 염소량이 높은 경우 처리 후 소독 부산물 문제 및 잔류 염소 문제를 유발하는데, 하기 실험예에 따르면, 본 발명 항균제 조성물은 유효 염소량이 적음에도, 우수한 항균 효능을 보인다.The antibacterial composition of the present invention preferably contains 4.14 to 5.06 ppm of available chlorine and 0.08 to 0.12% (v/v) of lactic acid. If the amount of available chlorine is high, problems of disinfection by-products and residual chlorine occur after treatment. However, according to the following experimental example, the antibacterial composition of the present invention exhibits excellent antibacterial efficacy even though the amount of available chlorine is small.
본 발명에서 사용되는 '수돗물을 전기분해하여 수득한 전해수'는 수돗물을 전기분해해서 수득할 수 있는데, 바람직하게는 도 1에 기재된 전기분해장치를 사용할 수 있다. 수돗물은 주변에서 가장 쉽게 구할 수 있는 염소 함유 수용액인데, 본 발명에서는 이를 전기분해해서 사용하는 것이다. 이렇게 수득한 수돗물 기반 중성 전해수를 젖산 수용액과 같이 사용함으로써 낮은 염소량과 약산성의 pH임에도 불구하고 우수한 항균능을 발휘하는 항균제 조성물을 제조할 수 있었다. The 'electrolyzed water obtained by electrolyzing tap water' used in the present invention can be obtained by electrolyzing tap water, and preferably, the electrolysis device described in Fig. 1 can be used. Tap water is a chlorine-containing aqueous solution that is most easily available in the surroundings, and in the present invention, it is used by electrolyzing it. By using the tap water-based neutral electrolyzed water obtained in this way together with a lactic acid aqueous solution, it was possible to manufacture an antibacterial composition that exhibits excellent antibacterial activity despite a low chlorine content and weakly acidic pH.
한편, 본 발명에서는 젖산 수용액을 사용하는데, 바람직하게 항균제 조성물 중 최종 농도 0.08~0.12 %(v/v)가 되도록 첨가하는 것이 좋다. 즉, 상기에서 제조한 '수돗물 기반 전해수'와 1 대 1의 양으로 혼합하여 본 발명의 항균제 조성물을 제조할 경우, 0.16~0.24 %(v/v) 농도의 젖산 수용액을 사용하면 되는 것이다. Meanwhile, in the present invention, a lactic acid aqueous solution is used, and it is preferably added so that the final concentration in the antibacterial composition is 0.08 to 0.12% (v/v). That is, when the antibacterial composition of the present invention is prepared by mixing the 'tap water-based electrolyzed water' prepared above in a 1:1 ratio, a lactic acid aqueous solution having a concentration of 0.16 to 0.24% (v/v) can be used.
한편, 본 발명은 상기 본 발명의 항균제 조성물을 사용하여 스테인리스 스틸(Stainless steel)을 살균하는 것을 특징으로 하는 스테인리스 스틸(Stainless steel)의 살균 방법을 제공한다. 가공 장비의 식품 접촉 표면에 사용되는 다양한 유형의 재료 중 스테인리스 스틸은 기계적 강도, 내식성, 수명 및 제조 용이성 때문에 가장 널리 사용된다. Meanwhile, the present invention provides a method for sterilizing stainless steel, characterized in that it sterilizes stainless steel using the antimicrobial composition of the present invention. Among various types of materials used for food contact surfaces of processing equipment, stainless steel is the most widely used due to its mechanical strength, corrosion resistance, lifespan, and ease of manufacturing.
그런데, 대표적인 식중독 균인 이콜라이 O157:H7 (Escherichia coli O157:H7), 살모넬라 타이피무리움 (Salmonella typhimurium), 리스테리아 모노사이토제네스 (Listeria monocytogenes)는 스테인리스 스틸 (Stainless steel) 표면에 부착되면 몇 시간 또는 며칠 동안 활성 상태를 유지할 수 있어 심각한 문제를 야기할 수 있다.However, representative food poisoning bacteria such as Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes can remain active for hours or days when attached to the surface of stainless steel, which can cause serious problems.
하지만, 스테인리스 스틸은 산성도가 높은 항균제를 사용할 경우, 부식하는 문제가 있는데, 본 발명의 경우 산성도가 낮아 부식의 문제가 없고, 그럼에도 불구하고 상기 오염 식중독 균에 대해 우수한 살균능을 발휘함을 하기 본 발명의 실험을 통해 확인하였다. However, stainless steel has a problem of corrosion when using an antibacterial agent with high acidity, but in the case of the present invention, since the acidity is low, there is no problem of corrosion, and despite this, it has been confirmed through the experiment of the present invention that it exhibits excellent sterilizing ability against the above-mentioned contaminated food poisoning bacteria.
이하, 본 발명의 내용을 하기 실시예 및 실험예를 통하여 보다 상세하게 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고 그와 등가의 기술적 사상의 변형까지를 포함한다.Hereinafter, the contents of the present invention will be described in more detail through the following examples and experimental examples. However, the scope of the rights of the present invention is not limited to the following examples and experimental examples, but includes modifications of technical ideas equivalent thereto.
[실시예 1: 본 발명의 항균제 조성물 제조][Example 1: Preparation of antibacterial composition of the present invention]
본 실시예에서는 본 발명에서 제조한 수돗물 기반 중성 전해수 및 젖산 수용액을 혼합하여 본 발명의 항균제 조성물을 제조하였다.In this example, the tap water-based neutral electrolyzed water and lactic acid aqueous solution manufactured in the present invention were mixed to manufacture the antibacterial composition of the present invention.
본 발명의 수돗물 기반 중성 전해수는 전해수 생성 장치를 사용해 수돗물을 전기분해하여 제조할 수 있었다. 전해수 생성 장치는 멤브레인(membrane)이 없는 220 mL 원통형 챔버 (높이: 175 mm, 직경: 64 mm)형태이고, 두 개의 백금 도금 티타늄 전극이 있는 모듈이 챔버 내부에 있으며, 양극과 음극 전극 사이의 간격은 13 mm인 장치이다 (도 1 참조). 상기 전극에 가해지는 전류와 전압을 각각 1.5 A, 24 V 로 설정한 후, 수돗물을 6분간 전기분해하여 중성 전해수 (tap water-based neutral electrolyzed water; TNEW)을 제조하였다. 한편, 상기 제조한 중성 전해수의 경우, pH가 7.70 ± 0.18인 것으로 나타났다. The tap water-based neutral electrolyzed water of the present invention can be produced by electrolyzing tap water using an electrolyzed water generating device. The electrolyzed water generating device is a membrane-less 220 mL cylindrical chamber (height: 175 mm, diameter: 64 mm) type, and a module having two platinum-plated titanium electrodes is located inside the chamber, and the gap between the anode and cathode electrodes is 13 mm (see Fig. 1). After setting the current and voltage applied to the electrodes to 1.5 A and 24 V, respectively, tap water was electrolyzed for 6 minutes to produce neutral electrolyzed water (tap water-based neutral electrolyzed water; TNEW). Meanwhile, the produced neutral electrolyzed water was found to have a pH of 7.70 ± 0.18.
이후, 상기 제조한 중성 전해수 및 0.2%(v/v) 젖산 수용액을 1:1 부피 비율로 혼합하여, 본 발명의 항균제 조성물을 제조하였다. 한편, 본 발명 항균제 조성물은, pH는 6.44 ± 0.14, 산화 환원 전위 (ORP)는 758.43 ± 28.53 mV, 유효 염소 농도는 4.60 ± 0.02 ppm 으로 나타났다.Thereafter, the neutral electrolyzed water and 0.2% (v/v) lactic acid aqueous solution prepared above were mixed in a volume ratio of 1:1 to prepare an antibacterial composition of the present invention. Meanwhile, the antibacterial composition of the present invention had a pH of 6.44 ± 0.14, an oxidation-reduction potential (ORP) of 758.43 ± 28.53 mV, and an available chlorine concentration of 4.60 ± 0.02 ppm.
[실험예 1: 본 발명 항균제 조성물의 항균 효능 검증 실험][Experimental Example 1: Experiment to verify the antibacterial efficacy of the antibacterial composition of the present invention]
본 실험예에서는 본 발명 항균제 조성물의 항균 효능을 검증하고자 했다.In this experimental example, the antibacterial efficacy of the antibacterial composition of the present invention was verified.
이를 위해, 스테인리스 스틸에 식중독 균을 접종한 후, 본 발명 항균제 조성물을 처리하여 항균 효능을 확인하였다. 구체적인 실험방법은 하기와 같다.To this end, after inoculating food poisoning bacteria on stainless steel, the antibacterial composition of the present invention was treated to confirm the antibacterial efficacy. The specific experimental method is as follows.
(1) 균주 접종(1) Strain inoculation
먼저 식중독 유발 균인 이콜라이 O157:H7 (Escherichia coli O157:H7), 살모넬라 타이피무리움 (Salmonella Typhimurium), 리스테리아 모노사이토제네스 (Listeria monocytogenes)를 각각 TSB 배지 (tryptic soy broth)에서 24시간 동안 배양하였다. 원심분리하여 펠렛(pellet)을 얻고, 상기 펠렛을 0.2% 멸균 펩톤수 (sterile peptone water, PW)에 희석하고, 스테인리스 스틸에 접종하였다. First, food poisoning-causing bacteria such as Escherichia coli O157:H7, Salmonella Typhimurium , and Listeria monocytogenes were cultured in tryptic soy broth (TSB) for 24 hours, respectively. Centrifugation was used to obtain a pellet, which was diluted in 0.2% sterile peptone water (PW), and inoculated onto stainless steel.
(2) 항균제 조성물 처리(2) Treatment with antibacterial composition
상기 스테인리스 스틸에 본 발명의 항균제 조성물 (실시예 1)을 분주 한 후, 30초마다 교반해주며, 1, 3, 5분간 처리하였다. 이후, 각 처리 시간에 따른 샘플들로부터 미생물 수를 계수하였다. After dispensing the antimicrobial composition of the present invention (Example 1) onto the stainless steel, it was stirred every 30 seconds and treated for 1, 3, and 5 minutes. Thereafter, the number of microorganisms was counted from samples according to each treatment time.
한편, 정확한 항균 효능을 확인하기 위해, 항균제 조성물 (실시예 1) 처리 이후 2시간의 회복과정을 거친 후 나타나는 항균 효능을 다시 확인하고자 했다. 이때는 처리샘플을 0.1 % (w/v) sodium pyruvate, 0.6 % (w/v) yeast extract를 포함한 TSB 배지내에 2시간 배양 후 미생물 수를 계수하였다.Meanwhile, in order to confirm the exact antibacterial efficacy, the antibacterial efficacy was re-confirmed after a 2-hour recovery period following treatment with the antibacterial composition (Example 1). At this time, the treated sample was cultured for 2 hours in TSB medium containing 0.1% (w/v) sodium pyruvate and 0.6% (w/v) yeast extract, and then the number of microorganisms was counted.
한편, 대조군으로 수돗물을 전기분해하여 제조한 것으로 유효염소 농도가 4.6 ppm인 수돗물 기반 중성 전해수 및 0.1%(v/v) 젖산 수용액을 각각 사용하였다.Meanwhile, as a control group, tap water-based neutral electrolyzed water with an effective chlorine concentration of 4.6 ppm and 0.1% (v/v) lactic acid solution were used, manufactured by electrolyzing tap water.
(3) 항균 효능 확인(3) Confirmation of antibacterial efficacy
중화가 끝난 이후, 선택 분별용 배지인 SMAC 한천배지 (sorbitol MacConkey agar), XLD 한천배지 (xylose lysine desoxycholate agar), OAB 한천배지 (Oxford agar base with Bacto Oxford antimicrobial supplement)에 희석하여 분주 도말 하고, 24시간 배양한 후, 배지 위에 선택적으로 형성된 콜로니를 계수하여, 항균제 처리 후 농도를 구했다. 처리 전, 후 농도를 이용하여 항균 효능을 확인하였다 (표 1). 또한, 더욱 정확한 항균 효능을 확인하기 위해 항균제 처리 후, 0.1 % (w/v) sodium pyruvate, 0.6 % (w/v) yeast extract를 포함한 TSB 배지내에 2시간이 지난 시점의 미생물 균수를 확인했다 (표 2). After neutralization, the solution was diluted and plated on selective differentiation media such as SMAC agar (sorbitol MacConkey agar), XLD agar (xylose lysine desoxycholate agar), and OAB agar (Oxford agar base with Bacto Oxford antimicrobial supplement), and after culturing for 24 hours, the colonies selectively formed on the media were counted and the concentration after antibiotic treatment was calculated. The antibacterial efficacy was confirmed using the concentrations before and after treatment (Table 1). In addition, to confirm more accurate antibacterial efficacy, the number of microbial cells was confirmed 2 hours after antibiotic treatment in TSB medium containing 0.1% (w/v) sodium pyruvate and 0.6% (w/v) yeast extract (Table 2).
(min)Processing time
(min)
(유효 염소 4.6 ppm)Neutral electrolyzed water based on tap water
(4.6 ppm available chlorine)
젖산 수용액0.1 %(v/v)
Lactic acid solution
(유효 염소 4.6 ppm, 젖산 0.1 %(v/v))Example 1
(available chlorine 4.6 ppm, lactic acid 0.1 %(v/v))
monocytogenesmonocytogenes
(min)Processing time
(min)
후 2시간Example 1 Processing
After 2 hours
monocytogenesmonocytogenes
상기 표 1을 보면, 본 발명의 항균제 조성물 (실시예 1)을 5분간 처리하는 경우 99.99%(4 log) 이상 살균되는 것을 확인할 수 있다. 반면, 중성 전해수 단독 처리 또는 젖산 수용액 단독 처리 군의 경우, 0.44~0.65 log 또는 0.21~0.34 log의 낮은 살균 효능을 보였다. 이는 본 발명의 항균제 조성물은 중성 전해수 및 젖산 수용액이 합쳐져 시너지 효과를 냈음을 의미한다. Looking at the above Table 1, it can be confirmed that when the antimicrobial composition of the present invention (Example 1) is treated for 5 minutes, sterilization is achieved by 99.99% (4 log) or more. On the other hand, in the case of the group treated with only neutral electrolyzed water or only lactic acid aqueous solution, a low sterilization efficacy of 0.44 to 0.65 log or 0.21 to 0.34 log was shown. This means that the antimicrobial composition of the present invention produced a synergistic effect by combining neutral electrolyzed water and lactic acid aqueous solution.
상기 표 2를 보면, 본 발명의 항균제 조성물을 처리한 후, 중화 이후 2시간이 지난 시점의 농도가 유의미한 차이를 보이지 않았는데, 이는 본 발명의 항균제 조성물을 1, 3, 5분 동안만 처리했음에도, 살균 효과가 우수하여 식중독 유발 균주가 회생되지 않았음을 의미한다.As shown in Table 2 above, after treatment with the antimicrobial composition of the present invention, the concentration at 2 hours after neutralization did not show a significant difference, which means that even though the antimicrobial composition of the present invention was treated for only 1, 3, and 5 minutes, the sterilization effect was excellent and the food poisoning-causing strains were not regenerated.
Claims (4)
유효 염소를 4.14~5.06 ppm, 젖산을 0.08~0.12 %(v/v) 농도로 포함하는 것을 특징으로 하는 항균제 조성물.
It is manufactured by mixing neutral electrolyzed water with a pH of 7.52 to 7.88 obtained by electrolyzing tap water and a lactic acid solution.
An antibacterial composition characterized by containing 4.14 to 5.06 ppm of available chlorine and 0.08 to 0.12 % (v/v) of lactic acid.
A method for sterilizing stainless steel, characterized by sterilizing stainless steel using the antimicrobial composition of claim 1.
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