KR102532432B1 - Method for preventing hair loss using protein inside of hair follicle - Google Patents

Abstract

The present invention relates to a method for preventing hair loss using proteins in hair follicles, and more specifically, proteins generated in hair follicles and a capsule layer coated with an emulsifier react to form a gel, filling the inside of hair follicles with neutralized protein to hold the hair roots, thereby preventing hair loss. It prevents hair loss by reinforcing the support that prevents hair loss from escaping from the hair follicle, and various nutrients provided inside the capsule layer are absorbed into the skin inside the hair follicle to improve elasticity and muscle strength of the skin inside the hair follicle, thereby strengthening the hair root and providing nutrients to the hair. is supplied to make hair thicker and stronger to prevent hair loss.

Description

Method for preventing hair loss using protein inside of hair follicle}

The present invention relates to a method for preventing hair loss using protein in the hair follicle, and more specifically, the protein produced in the hair follicle reacts with the capsule layer coated with a weakly acidic emulsifier to form a gel, filling the hair follicle with neutralized protein to stimulate the hair root By holding it, it reinforces the supporting power that prevents hair from escaping from the hair follicle to prevent hair loss, and various nutrients provided inside the capsule layer are absorbed into the skin inside the hair follicle, improving the elasticity and muscle strength of the skin inside the hair follicle, thus improving the hair root. It relates to a method for preventing hair loss using protein in the hair follicle, which can prevent hair loss by making the hair thick and strong by supplying nutrients to the hair.

Alopecia is influenced by various factors, namely genetic, hormonal and environmental factors, by diet, and by physical activity. Hair has an essential aesthetic identity role. Thus, alopecia in women or men can cause considerable psychological distress.

Thus, healthy, strong hair and thick hair throughout life are the aspirations of most women and men. Many techniques are known for the treatment of alopecia, such as cell therapy, laser therapy or otherwise non-surgical implants. To obtain implants, human hair follicles are obtained by culturing various cell types present in hair follicles.

A hair follicle refers to a bag-shaped epithelial tissue that surrounds a hair root, and a hair root refers to a part where hair is embedded in the skin. Hair grows by actively cell division near the hair follicle. If this cell division ability is lowered, the hair follicles will atrophy and migrate to shallow areas of the skin. Normally, the cell division ability is regained, and the generated new cell group moves deep into the skin to form new hair follicles and grow hair to push out the old hair. If this 'hair exchange' does not occur, alopecia occurs. .

The optimal treatment method for hair loss developed so far is hair transplantation, but in the early stages of hair loss, external use Minoxidil sold on the market after FDA approval or Propecia, which contains finasteride as the main ingredient, is also used for hair loss. may slow down progress.

In order to find effective substances useful for early hair loss treatment, mice were depilated, samples were applied to the back, and hair growth was observed. Animal experiments have been carried out to confirm the effects of growth factors such as Epidermal growth factor (Epidermal growth factor) and HGF (Hepatocyte growth factor). All of these did not match. On the other hand, in the case of the monkey model, the tendency is similar to that of humans, but the burden of time and experiment cost is too great. In addition, recently, in vivo experiments using animals have been socially criticized and legally regulated in terms of animal protection, so the development of an ex vivo toxicity test model to replace animal tests has emerged as a global problem.

Unlike in vivo experiments, in the case of in vitro experiments, long-term experiments are difficult because nutrition or oxygen supply through blood vessels is blocked during cell culture. In addition, since the experiment may be terminated due to rapid cell death of the artificial tissue before confirming the effect of the active ingredient, it is most important to maintain the function of the artificial tissue for a long period of time by creating an environment similar to the living body in the case of an in vitro experiment. .

As an in vitro hair growth model using hair follicle organ culture, 10% by weight of serum was added to DMEM (Dulbecco's Modified Eagle Medium) by immersion culture and then cultured in a glass bottle, oxygen pressure was increased and cultured in RPMI 1640 medium at 31 ° C. In the case of culturing at 37 ° C., which is a general culture temperature, the length growth rate and length increase are reduced, but the morphological change of hair bulbs is less.

On the other hand, various types of hair loss prevention and hair growth agents are generally combined drugs applied to the scalp, mainly for the purpose of promoting blood circulation in the scalp, activating hair follicle cells, suppressing lipid secretion in the scalp, supplying hair nutrients, etc. It has been used. .

However, the current situation is that there is almost no hair growth effect without exception, and even what is called a hair growth agent seems to have an unexpected expectation while the user cannot recognize the effect.

Furthermore, because hormones of inappropriate concentration are used, skin inflammation is caused, and hair growth may not occur due to stimulation of alcohol combined together, and hair loss may also occur.

Republic of Korea Patent Publication No. 1999-0078804 Republic of Korea Patent Publication No. 2000-0024499

The present invention, which was devised in view of the above conventional problems,

First, when applied to the scalp, the protein and keratin generated on the surface of the scalp react with the capsule layer composed of the weakly acidic modified emulsifier to easily remove the keratin from the scalp, so that the scalp can be managed cleanly,

Second, the protein produced in the hair follicle and the capsule layer coated with the emulsifier modified with weak acidity react to form a gel, filling the hair follicle and holding the hair root to reinforce the support that prevents hair from escaping from the hair follicle to prevent hair loss. there is,

Third, various nutrients provided inside the capsule layer are absorbed by the scalp and the epidermis in the hair follicle to improve the elasticity and strength of the skin in the scalp and hair follicle, thereby strengthening the hair follicle and supplying nutrients to the hair to prevent hair loss by making the hair thick and strong. It is to provide a method for preventing hair loss using a protein in the hair follicle that can be used.

An object of the present invention is to prevent hair loss in a method for preventing hair loss, in which the protein in the hair follicle and the composition for preventing hair loss supplied in the hair follicle react with the protein in the hair follicle when the anti-hair loss composition is put into the hair follicle, and the hair follicle is filled with a gel-like reactant to prevent hair loss It is achieved by a method for preventing hair loss using proteins in hair follicles.

The hair loss prevention composition is composed of a mixture of at least two of a caffeine extract used as a base, a hair promoting extract having a hair promoting component, a skin irritation relieving extract having a skin irritation relieving component, and a skin regeneration extract having a skin regeneration effect. It is achieved by a method for preventing hair loss using protein in the hair follicle, characterized in that it is made by liposomizing the mixed nutritional extract into nano-size and then mixing the weakly acidic modified emulsifier.

40 to 10% by weight of caffeine extract, 35 to 45% by weight of hair promoting extract, 10 to 17% by weight of skin irritation relief extract, and 15 to 23% by weight of skin regeneration extract are mixed with respect to 100% by weight of the mixed nutritional extract It is achieved by a method for preventing hair loss.

The emulsifier is achieved by a hair loss prevention method using a protein in the hair follicle, characterized in that any one of lecithin, sorbitan, glyceryl stearate, and phospholipid.

The emulsifier is achieved by a method for preventing hair loss using a protein in the hair follicle, characterized in that 2.0 to 5.0% by weight is mixed.

It is achieved by a hair loss prevention method using a protein in the hair follicle, characterized in that the viscosity of the gel-formed reactant is in the range of 1,200 to 4,400cps.

The fermentation process is achieved by a hair loss prevention method using protein in the hair follicle, characterized in that fermenting bacteria is mixed at 10 to 20% by weight with respect to 100% by weight of the extract and fermented for 24 to 72 hours at a temperature of 35 to 40 ° C. .

As such, the present invention

First, when applied to the scalp, the capsule layer composed of the emulsifier modified with weak acidity, such as protein and keratin, etc., reacted on the surface of the scalp, so that the keratin can be easily removed from the scalp, so that the scalp can be managed cleanly,

Second, the protein produced in the hair follicle and the capsule layer coated with the emulsifier modified with weak acidity react to form a gel, filling the hair follicle and holding the hair root to reinforce the support that prevents hair from escaping from the hair follicle to prevent hair loss. there is,

Third, various nutrients provided inside the capsule layer are absorbed by the scalp and the epidermis in the hair follicle, and the elasticity and muscle strength of the scalp and the skin in the hair follicle are improved. As a result, the hair follicle is strengthened and nutrients are supplied to the hair to prevent hair loss by supplying nutrients to the hair. It is a very useful invention that has such an effect.

1 is a manufacturing process diagram showing a manufacturing method of an anti-hair loss composition to which the technology of the present invention is applied.
Figure 2 is a photograph showing the reaction of the anti-hair loss composition and protein prepared by the present invention.

Hereinafter, a preferred embodiment of the present invention will be described in detail based on the accompanying drawings.

Figure 1 is a manufacturing process diagram showing a manufacturing method of a hair loss prevention composition using the protein in the hair follicle to which the technology of the present invention is applied. Caffeine extract used as a base, hair promoting extract extracted from the 1st natural plant group having a hair stimulating ingredient, skin irritation relieving extract extracted from the 2nd natural plant group having a skin irritation relieving ingredient, and skin regenerating effect A raw material preparation step (S100) of preparing a mixed nutritional extract by mixing the skin regeneration extract extracted from the third natural plant group is performed.

The raw material preparation step (S100) is a natural plant capable of extracting caffeine, and the first natural flora group consisting of goji berry, licorice, dermis, jujube, perilla leaf, ginseng, black soybean, baekhasuo, houttuynia cordata, green tea, and green tea , Ginkgo leaf, persimmon leaf, Cornus officinalis fruit, dandelion, perilla leaf, deodeok, quince, mulberry fruit, bellflower, licorice, etc., the second natural flora family, composed of thistle, Schisandra chinensis, dermis, jujube, brown rice, root of tuna, kelp, etc. After cleaning the third natural plant group and drying it at 60 ° C for 24 to 48 hours, cut it into a certain size and dry it, put each natural plant and natural plant group in a clean hemp bag and dry it again for about 24 hours. Place the burlap bag in a container of distilled or water and heat it. At this time, the extract is collected by collecting the generated water vapor.

At this time, 40 to 10% by weight of caffeine extract, 35 to 45% by weight of hair promoting extract, 10 to 17% by weight of skin irritation relief extract, and 15 to 23% by weight of skin regeneration extract are mixed with respect to 100% by weight of the mixed nutritional extract. It has that characteristic.

The reason why the extraction method used in the raw material preparation step (S100) is limited to the steam extraction method is that no residual solvent is generated, there is no difficulty in selecting a solvent, and the extraction method is simple, low-cost, and highly efficient.

On the other hand, a fermentation step (S200) of fermenting the mixed nutritional extract by adding a Bacillus fermentation strain to the mixed nutritional extract prepared by mixing the respective extracts obtained in the raw material preparation step (S100) is performed.

In the fermentation step (S200) of fermenting the mixed nutritional extract, 10 to 20% by weight of fermenting bacteria is mixed with respect to 100% by weight of the extract, and fermented for 24 to 72 hours at a temperature of 35 to 40 ° C.

The reason for fermenting the mixed nutrient extract as described above is to change the mixed nutrient extract having a high-molecular matrix into a low-molecular matrix to improve the scalp (skin) absorption and antibacterial power, and the scalp (skin) adsorbability, scalp (blood) It can be confirmed in Table 1 below for the degree of irritation, hair elasticity, and scalp improvement.

[Experiment 1]

<Experimental example>

100ml of a mixed nutritional extract was prepared by mixing 25.0% by weight of caffeine extract, 40.0% by weight of hair stimulating extract, 15.0% by weight of skin irritation relief extract, and 20.0% by weight of skin regeneration extract.

<Comparative Example 1>

A total of 30 people, 15 men and 15 women, tested the unfermented mixed nutritional extract for scalp (skin) absorption, scalp (skin) sub-irritation, and hair elasticity (5-point measurement method). Regarding the degree of scalp improvement, it is the result of measuring with a portable scalp measuring device at 5-day intervals for 30 days.

<Example 1>

100ml of the mixed nutrient extract fermented from the above mixed nutrient extract was prepared, and a total of 30 people, 15 males and 15 females, conducted a sensory evaluation on sensory scalp (skin) absorption, scalp (skin) stimulation, and hair elasticity. It is shown in Table 1 below. Regarding the degree of scalp improvement, it is the result of measuring with a portable scalp measuring device at 5-day intervals for 30 days.

Scalp absorption scalp irritation hair elasticity Scalp improvement Comparative Example 1
U.S. fermented mixed nutritional extract (conventional) very little little irritation commonly 4.13 Example 1
Fermented mixed nutritional extract (the present invention) speed no irritation good 4.82

Experiment method: Disk Diffusion method

By the fermentation process (S200) of fermenting the mixed nutrient extract obtained by the raw material preparation process (S100), cells and foreign substances mixed in the fermentation broth are adsorbed and the filtered water separated and purified is centrifuged to produce a high-concentration fermented extract. A high-concentration fermentation extract acquisition step (S300) is performed.

In the high-concentration fermentation extract acquisition step (S300), foreign substances are removed from the fermentation broth after the fermentation step (S200) has been completed using an ion exchange resin having weak acidity, and the fermentation broth that has passed through the ion exchange resin is put into a centrifuge and centrifuged to separate the previous process. The particulate foreign matter and cells that could not be removed from the chamber undergo phase separation in the lower part, and the solution containing the active ingredient in the upper part. The supernatant is separated in the centrifugal separator where the phase separation has occurred, and stored in a container for 1-2 days and matured, resulting in a highly concentrated mixture. Obtain nutrient extracts.

A nanoization process (S400) is performed to safely and stably deliver the active ingredients of the high-concentration mixed nutritional extract obtained as described above to the dermal layer of the skin and prepare them in a size of 10 to 50 nm for easy skin absorption.

In the nano-process (S400), the mixed nutrient extract is brought to a temperature of 40 to 50° C., and then the mixed nutrient extract having the above temperature is put into a pad mixer, stirred slowly at 60 to 100 rpm, and then stirred at 2,500 to 3,500 rpm using a homo mixer. After stirring for about 2 to 4 minutes and completing the stirring operation, the process is repeated three times at a pressure of 700 to 1,000 bar in a microfluidizer.

On the other hand, as shown in FIG. 2 of the accompanying drawings, looking at the difference in skin absorption of the mixed nutritional extract that has not been subjected to the nanoization process (S400) and the mixed nutritional extract that has undergone the nanoization process (S400), the nanoization process (S400) as in the present invention It can be confirmed that the skin absorption power of the mixed nutrient extract performed was significantly higher.

A mixing encapsulation step (S500) of mixing and encapsulating the high-concentration mixed nutrient extract and the weakly acidic emulsifier after the nanoization step (S400) is completed is performed. When mixing the emulsifier, when the emulsifier does not show a pH range of 5.5 to 6.5, it is preferable to add a pH adjuster to adjust the acidity to the emulsifier to have weak acidity. The reason is that if the pH is less than 5.5, there is a possibility of damaging the hair and skin, and if the pH is more than 6.5, there is a problem that the state change in the form of a gel does not occur because the reaction cannot be made with the protein.

As shown in FIG. 2, the protein in the hair follicle reacts with the capsule layer coated with the weakly acidic emulsifier to form a gel, showing that the inside of the hair follicle is filled with neutralized protein. While holding it, various nutrients inside the capsule layer are absorbed into the skin inside the hair follicle, improving the elasticity and muscle strength of the skin inside the hair follicle, thus strengthening the hair root, and supplying nutrients to the hair to make the hair thicker and stronger.

On the other hand, in the mixing encapsulation step (S500), the emulsifier uses lecithin extracted from soybeans, eggs, etc., and sorbitan, glyceryl stearate, phospholipid (Lecithin) is used instead of lecithin Phospolipid) can be used.

In the mixing encapsulation process (S500), when 2.0 to 5.0% by weight of an emulsifier is added to 100% by weight of the high-concentration fermented extract, the emulsifier modified to be weakly acidic forms a coating layer, that is, a capsule, on the surface of the capsule made of the emulsifier. Inside, a high concentration of mixed nutrient extract is contained.

If the emulsifier is used at 2.0% by weight or less, the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the weakly acidic emulsifier does not form sufficient viscosity and is in an unstable state, so it does not properly function as an emulsifier. If 5.0% by weight or more of the emulsifier is used, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier becomes high, exceeding the appropriate range, and the emulsifiers stick together. This is because there is a problem in that non-uniform crystals occur.

A dual liposome process (S600) is performed to re-nanoize the size of the encapsulated mixture. The dual liposome process (S600) performs the same process as the nanoization process (S400) once more, and process description is omitted, and the molecular size is 50 to 120 nm. It has a structure coated with a double layer.

The reason for encapsulating the mixed nutritional extract as described above is to ensure that the mixed nutritional extract can be easily supplied into the hair follicles when the mixed nutritional extract is applied or sprayed on the scalp, and into the hair follicles, the hair loss prevention composition of the present invention Table 3 below shows the changes in the hair follicle when it enters, and the reaction rate of the protein according to the acidity of the emulsifier is examined in Table 4.

<Comparative Example 2>

After preparing 100 ml of the mixed nutrient extract subjected to the nano-fermentation process of the unfermented mixed nutrient extract (Comparative Example 1) and mixing 2 ml of protein peptide with the nano-fermented unfermented mixed nutrient extract, the reaction was examined over time.

<Example 2>

After mixing 2 ml of pH 5.5 emulsifier with 100 ml of high-concentration fermented extract subjected to nanoization process and mixing 2 ml of protein peptide, the reaction was examined over time.

<Example 3>

After mixing 2 ml of an emulsifier of pH 6.0 with 100 ml of high-concentration fermented extract subjected to the nanoization process, performing a dual liposome process, mixing 2 ml of protein peptide, and then examining the viscosity over time.

1 minute elapsed
(viscosity or photo) 2 minutes elapsed 3 minutes elapsed 5 minutes elapsed Comparative Example 2 1,200 cps 1,250 cps 1,500 cps 1,900 cps Example 2 1,300 cps 1,500 cps 1,900 cps 2,600 cps Example 3 1,600 cps 2,200 cps 3,000 cps 4,200 cps

Experiment : Brookfield DV2TL VTJO Spindle : 62, Speed : 30rpm, Temperature : 25℃, Time : 30sec

Acidity of Emulsifier Tensile force when pulling hair after reaction pH 5.5 pH 5.8 pH 6.0 pH 6.5 Comparative Example 2 1,750 cps 1,900 cps 1,900 cps 2,200 cps commonly Example 2 2,200 cps 2,500 cps 2,600 cps 2,900 cps strong Example 3 3,500 cps 4,100 cps 4,200 cps 4,400 cps very strong

Experiment: Measured after aging for 72 hours at 25℃ in a thermostat

Brookfield DV2TL VTJO Spindle : 62, Speed : 30rpm, Temperature :

25℃, Time : 30sec

When the emulsifier and the protein react, the viscosity of the gel-type reactant is preferably in the range of 1,200 to 4,400 cps, and when the viscosity is 1,200 cps or less, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier is too low. There is a problem in that the formation of the gel is low and the power to hold the hair root is insufficient, so that hair loss cannot be prevented.

On the other hand, if the viscosity is 　4,400cps　 or more, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier is too high, so the gel filled in the hair follicle may block the hair follicle and cause scalp trouble. Various nutrients that have been released are not sufficiently absorbed by the scalp and hair, so they cannot function to prevent hair loss.

As such, when the present invention is applied to the scalp, the capsule layer composed of emulsifiers such as protein and keratin, etc. reacted on the surface of the scalp, so that keratin can be easily removed from the scalp, so that the scalp can be managed cleanly, and the generated in the hair follicle The capsule layer coated with protein and emulsifier reacts to form a gel, filling the hair follicles and holding the hair roots to reinforce the supporting power that prevents hair from escaping from the hair follicles to prevent hair loss, and the inside of the capsule layer on the scalp and epidermis in the hair follicles It is a very useful invention that has effects such as the absorption of various nutrients provided in the scalp and the skin in the hair follicles to improve elasticity and muscle strength, thereby strengthening the hair follicles and supplying nutrients to the hair to prevent hair loss by making the hair thick and strong. .

Claims (8)

In the hair loss prevention method,
40 to 10% by weight of caffeine extract, 35 to 45% by weight of hair stimulating extract, 10 to 17% by weight of skin irritation relief extract, and 15 to 23% by weight of skin regeneration extract After liposomizing the extract to a size of 10 to 50 nanometers, the acidity is in the range of pH 5.5 to 6.5 and the anti-hair loss composition coated with an emulsifier of 2.0 to 5.0% by weight of the total weight is put into the hair follicle, and the protein in the hair follicle reacts with the slightly acidic emulsifier. A method for preventing hair loss using protein in the hair follicle, characterized in that the gel-like reactant is generated with a viscosity in the range of 1,200 to 4,400cps, so that the gel-like reactant holds the hair to prevent hair loss.
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